VCL cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCL
Entrez Gene ID	100064165
Full Name	vinculin
General protein information	Preferred Names vinculin Names vinculin
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014733026.1	XP_014588512.1	vinculin isoform X3
XM_014733027.1	XP_014588513.1	vinculin isoform X4
XM_014733024.1	XP_014588510.1	vinculin isoform X1
XM_014733025.1	XP_014588511.1	vinculin isoform X2
XM_014733027.2	XP_014588513.2	vinculin isoform X4
XM_014733024.2	XP_014588510.2	vinculin isoform X1
XM_014733025.2	XP_014588511.2	vinculin isoform X2
XM_014733026.2	XP_014588512.2	vinculin isoform X3

Pathways from BioSystems
Gene Ontology

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb28254	XM_014733024.1 Latest version!	Equus caballus vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OHb40250	XM_014733024.2 Latest version!	Equus caballus vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHb40251	XM_014733025.2 Latest version!	Equus caballus vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHb40252	XM_014733026.2 Latest version!	Equus caballus vinculin (VCL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHb40253	XM_014733027.2 Latest version!	Equus caballus vinculin (VCL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHb09770	XM_014733025.1 Latest version!	Equus caballus vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OHb09771	XM_014733026.1 Latest version!	Equus caballus vinculin (VCL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OHb09772	XM_014733027.1 Latest version!	Equus caballus vinculin (VCL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCL ( XM_014733024.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014588510.1 Equus caballus vinculin (VCL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHb28254
Clone ID Related Accession (Same CDS sequence)	XM_014733024.1
Accession Version	XM_014733024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTCCGTGA CCCCAGTGCC TCCCCAGGGG ATGCTGGTGA GCAAGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGCGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCTTGG GAACCTGCAA AATGCTAGGA CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGCGCCTC ACCAGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAAGGT 
CTAGATGTGC TCACAGCGAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGGGAT GACATTTTAC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC CCCAGAGGCC 
CGAGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTCCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATAGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACGG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAGCT CCAAGACTCT 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTTGCGG CCACTGCACC CCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGA GCAGCTAATT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCAGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CGTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTCGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC 
AAATCTTTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ATCTGGACAA GTGTAAAGTA 
GCCATGGCCA ATATTCAGCC TCAGATGCTG GTCGCTGGGG CAACCAGCAT TGCCCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAG TTGAGCAAAA CTATCTCCCC AATGGTAATG 
GATGCAAAGG CTGTGGCTGG AAACATTTCT GATCCTGGCC TGCAAAAGAG CTTCCTGGAC 
TCAGGATATA GGATCCTGGG AGCTGTTGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAGCAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGAGAG GTCCCTCCCC CCAGACCCCC ACCACCGGAG 
GAGAAGGACG AAGAGTTCCC TGAGCAGAAA GCTGGGGAGG TGATTAATCA GCCAATGATG 
ATGGCTGCCA GGCAACTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTGAGG TGGGTCTAAG TGTTGTAGCT GAGGCAGATG CGGCCGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GTTGATGGCT 
GAGATGTCTC GGCTGGTAAG AGGGGGCAGT GGTACCAAGC GGGCACTGAT TCAATGTGCC 
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGCATTAG AACCAACCTC TTACAGGTAT GTGAGCGAAT CCCCACCATA 
AGCACCCAGC TGAAAATCCT GTCCACAGTA AAGGCCACCA TGCTGGGCCG GACCAACATC 
AGTGATGAGG AATCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG 
CAGTCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TCGAACTGAT 
GCTGGATTTA CACTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014588510.1
CDS	145..3552
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAEVGLSVVAEADAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_014733024.1
Organism	Equus caballus(horse)
Definition	Equus caballus vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733024.1

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTCCGTGA CCCCAGTGCC TCCCCAGGGG ATGCTGGTGA GCAAGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGCGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCTTGG GAACCTGCAA AATGCTAGGA CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGCGCCTC ACCAGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAAGGT 
CTAGATGTGC TCACAGCGAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGGGAT GACATTTTAC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC CCCAGAGGCC 
CGAGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTCCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATAGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACGG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAGCT CCAAGACTCT 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTTGCGG CCACTGCACC CCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGA GCAGCTAATT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCAGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CGTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTCGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC 
AAATCTTTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ATCTGGACAA GTGTAAAGTA 
GCCATGGCCA ATATTCAGCC TCAGATGCTG GTCGCTGGGG CAACCAGCAT TGCCCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAG TTGAGCAAAA CTATCTCCCC AATGGTAATG 
GATGCAAAGG CTGTGGCTGG AAACATTTCT GATCCTGGCC TGCAAAAGAG CTTCCTGGAC 
TCAGGATATA GGATCCTGGG AGCTGTTGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAGCAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGAGAG GTCCCTCCCC CCAGACCCCC ACCACCGGAG 
GAGAAGGACG AAGAGTTCCC TGAGCAGAAA GCTGGGGAGG TGATTAATCA GCCAATGATG 
ATGGCTGCCA GGCAACTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTGAGG TGGGTCTAAG TGTTGTAGCT GAGGCAGATG CGGCCGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GTTGATGGCT 
GAGATGTCTC GGCTGGTAAG AGGGGGCAGT GGTACCAAGC GGGCACTGAT TCAATGTGCC 
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGCATTAG AACCAACCTC TTACAGGTAT GTGAGCGAAT CCCCACCATA 
AGCACCCAGC TGAAAATCCT GTCCACAGTA AAGGCCACCA TGCTGGGCCG GACCAACATC 
AGTGATGAGG AATCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG 
CAGTCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TCGAACTGAT 
GCTGGATTTA CACTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_014733024.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_014588510.2 Equus caballus vinculin (VCL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHb40250
Clone ID Related Accession (Same CDS sequence)	XM_014733024.2
Accession Version	XM_014733024.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014733024.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTCCGTGA CCCCAGTGCC TCCCCAGGGG ATGCTGGTGA GCAAGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGCGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCTTGG GAACCTGCAA AATGCTAGGA CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGCGCCTC ATCAGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAAGGT 
CTAGATGTGC TCACAGCGAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGGGAT GACATTTTAC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC CCCAGAGGCC 
CGAGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTCCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATAGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACGG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAGCT CCAAGACTCT 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTTGCGG CCACTGCACC CCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGA GCAGCTAATT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCAGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CGTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTCGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC 
AAATCTTTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ATCTGGACAA GTGTAAAGTA 
GCCATGGCCA ATATTCAGCC TCAGATGCTG GTCGCTGGGG CAACCAGCAT TGCCCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAG TTGAGCAAAA CTATCTCCCC AATGGTAATG 
GATGCAAAGG CTGTGGCTGG AAACATTTCT GATCCTGGCC TGCAAAAGAG CTTCCTGGAC 
TCAGGATATA GGATCCTGGG AGCTGTTGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAGCAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGAGAG GTCCCTCCCC CCAGACCCCC ACCACCGGAG 
GAGAAGGACG AAGAGTTCCC TGAGCAGAAA GCTGGGGAGG TGATTAATCA GCCAATGATG 
ATGGCTGCCA GGCAACTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTGAGG TGGGTCTAAG TGTTGTAGCT GAGGCAGATG CGGCCGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GTTGATGGCT 
GAGATGTCTC GGCTGGTAAG AGGGGGCAGT GGTACCAAGC GGGCACTGAT TCAATGTGCC 
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGCATTAG AACCAACCTC TTACAGGTAT GTGAGCGAAT CCCCACCATA 
AGCACCCAGC TGAAAATCCT GTCCACAGTA AAGGCCACCA TGCTGGGCCG GACCAACATC 
AGTGATGAGG AATCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG 
CAGTCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TCGAACTGAT 
GCTGGATTTA CACTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014588510.2
CDS	130..3537
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASSVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAEVGLSVVAEADAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_014733024.2
Organism	Equus caballus(horse)
Definition	Equus caballus vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733024.2

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTCCGTGA CCCCAGTGCC TCCCCAGGGG ATGCTGGTGA GCAAGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGCGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCTTGG GAACCTGCAA AATGCTAGGA CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGCGCCTC ATCAGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAAGGT 
CTAGATGTGC TCACAGCGAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGGGAT GACATTTTAC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC CCCAGAGGCC 
CGAGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTCCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATAGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACGG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAGCT CCAAGACTCT 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTTGCGG CCACTGCACC CCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGA GCAGCTAATT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCAGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CGTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTCGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC 
AAATCTTTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ATCTGGACAA GTGTAAAGTA 
GCCATGGCCA ATATTCAGCC TCAGATGCTG GTCGCTGGGG CAACCAGCAT TGCCCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAG TTGAGCAAAA CTATCTCCCC AATGGTAATG 
GATGCAAAGG CTGTGGCTGG AAACATTTCT GATCCTGGCC TGCAAAAGAG CTTCCTGGAC 
TCAGGATATA GGATCCTGGG AGCTGTTGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAGCAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGAGAG GTCCCTCCCC CCAGACCCCC ACCACCGGAG 
GAGAAGGACG AAGAGTTCCC TGAGCAGAAA GCTGGGGAGG TGATTAATCA GCCAATGATG 
ATGGCTGCCA GGCAACTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTGAGG TGGGTCTAAG TGTTGTAGCT GAGGCAGATG CGGCCGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GTTGATGGCT 
GAGATGTCTC GGCTGGTAAG AGGGGGCAGT GGTACCAAGC GGGCACTGAT TCAATGTGCC 
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGCATTAG AACCAACCTC TTACAGGTAT GTGAGCGAAT CCCCACCATA 
AGCACCCAGC TGAAAATCCT GTCCACAGTA AAGGCCACCA TGCTGGGCCG GACCAACATC 
AGTGATGAGG AATCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG 
CAGTCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TCGAACTGAT 
GCTGGATTTA CACTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_014733025.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_014588511.2 Equus caballus vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb40251
Clone ID Related Accession (Same CDS sequence)	XM_014733025.2
Accession Version	XM_014733025.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014733025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAGTGCCTCC CCAGGGGATG CTGGTGAGCA AGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGC GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCTTGGGAA CCTGCAAAAT GCTAGGACAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GCGCCTCATC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAAGGTCTA 
GATGTGCTCA CAGCGAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGGGATGAC ATTTTACGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TAGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACGGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCTTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTTGCGGCCA CTGCACCCCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAATTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTTTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGATC TGGACAAGTG TAAAGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC CCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAGTTG AGCAAAACTA TCTCCCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAT CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATAGGA TCCTGGGAGC TGTTGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAG CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGAGAGGTC CCTCCCCCCA GACCCCCACC ACCGGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCT GGGGAGGTGA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AACTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTGAGGTGG GTCTAAGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGTT GATGGCTGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA ATGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GCATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC CACCATAAGC 
ACCCAGCTGA AAATCCTGTC CACAGTAAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAAT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACTGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014588511.2
CDS	129..3533
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASSVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAEVGLSVVAEADAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_014733025.2
Organism	Equus caballus(horse)
Definition	Equus caballus vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733025.2

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAGTGCCTCC CCAGGGGATG CTGGTGAGCA AGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGC GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCTTGGGAA CCTGCAAAAT GCTAGGACAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GCGCCTCATC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAAGGTCTA 
GATGTGCTCA CAGCGAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGGGATGAC ATTTTACGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TAGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACGGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCTTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTTGCGGCCA CTGCACCCCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAATTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTTTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGATC TGGACAAGTG TAAAGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC CCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAGTTG AGCAAAACTA TCTCCCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAT CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATAGGA TCCTGGGAGC TGTTGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAG CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGAGAGGTC CCTCCCCCCA GACCCCCACC ACCGGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCT GGGGAGGTGA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AACTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTGAGGTGG GTCTAAGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGTT GATGGCTGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA ATGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GCATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC CACCATAAGC 
ACCCAGCTGA AAATCCTGTC CACAGTAAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAAT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACTGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_014733026.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_014588512.2 Equus caballus vinculin (VCL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHb40252
Clone ID Related Accession (Same CDS sequence)	XM_014733026.2
Accession Version	XM_014733026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	vinculin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014733026.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTCCGTGA CCCCAGTGCC TCCCCAGGGG ATGCTGGTGA GCAAGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGCGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCTTGG GAACCTGCAA AATGCTAGGA CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGCGCCTC ATCAGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAAGGT 
CTAGATGTGC TCACAGCGAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGGGAT GACATTTTAC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC CCCAGAGGCC 
CGAGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTCCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATAGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACGG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAGCT CCAAGACTCT 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTTGCGG CCACTGCACC CCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGA GCAGCTAATT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCAGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CGTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTCGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC 
AAATCTTTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ATCTGGACAA GTGTAAAGTA 
GCCATGGCCA ATATTCAGCC TCAGATGCTG GTCGCTGGGG CAACCAGCAT TGCCCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAG TTGAGCAAAA CTATCTCCCC AATGGTAATG 
GATGCAAAGG CTGTGGCTGG AAACATTTCT GATCCTGGCC TGCAAAAGAG CTTCCTGGAC 
TCAGGATATA GGATCCTGGG AGCTGTTGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAGCAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGAGAG GTCCCTCCCC CCAGACCCCC ACCACCGGAG 
GAGAAGGACG AAGAGTTCCC TGAGCAGAAA GCTGGGGAGG TGATTAATCA GCCAATGATG 
ATGGCTGCCA GGCAACTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGTTG ATGGCTGAGA TGTCTCGGCT GGTAAGAGGG 
GGCAGTGGTA CCAAGCGGGC ACTGATTCAA TGTGCCAAGG ACATCGCCAA GGCCTCAGAT 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG CATTAGAACC 
AACCTCTTAC AGGTATGTGA GCGAATCCCC ACCATAAGCA CCCAGCTGAA AATCCTGTCC 
ACAGTAAAGG CCACCATGCT GGGCCGGACC AACATCAGTG ATGAGGAATC TGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTCGA ACTGATGCTG GATTTACACT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014588512.2
CDS	128..3331
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASSVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_014733026.2
Organism	Equus caballus(horse)
Definition	Equus caballus vinculin (VCL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733026.2

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTCCGTGA CCCCAGTGCC TCCCCAGGGG ATGCTGGTGA GCAAGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGCGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCTTGG GAACCTGCAA AATGCTAGGA CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGCGCCTC ATCAGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAAGGT 
CTAGATGTGC TCACAGCGAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGGGAT GACATTTTAC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC CCCAGAGGCC 
CGAGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTCCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATAGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACGG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAGCT CCAAGACTCT 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTTGCGG CCACTGCACC CCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGA GCAGCTAATT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCAGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CGTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTCGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC 
AAATCTTTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ATCTGGACAA GTGTAAAGTA 
GCCATGGCCA ATATTCAGCC TCAGATGCTG GTCGCTGGGG CAACCAGCAT TGCCCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAG TTGAGCAAAA CTATCTCCCC AATGGTAATG 
GATGCAAAGG CTGTGGCTGG AAACATTTCT GATCCTGGCC TGCAAAAGAG CTTCCTGGAC 
TCAGGATATA GGATCCTGGG AGCTGTTGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAGCAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGAGAG GTCCCTCCCC CCAGACCCCC ACCACCGGAG 
GAGAAGGACG AAGAGTTCCC TGAGCAGAAA GCTGGGGAGG TGATTAATCA GCCAATGATG 
ATGGCTGCCA GGCAACTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGTTG ATGGCTGAGA TGTCTCGGCT GGTAAGAGGG 
GGCAGTGGTA CCAAGCGGGC ACTGATTCAA TGTGCCAAGG ACATCGCCAA GGCCTCAGAT 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG CATTAGAACC 
AACCTCTTAC AGGTATGTGA GCGAATCCCC ACCATAAGCA CCCAGCTGAA AATCCTGTCC 
ACAGTAAAGG CCACCATGCT GGGCCGGACC AACATCAGTG ATGAGGAATC TGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTCGA ACTGATGCTG GATTTACACT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_014733027.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_014588513.2 Equus caballus vinculin (VCL), transcript variant X4, mRNA.

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CloneID	OHb40253
Clone ID Related Accession (Same CDS sequence)	XM_014733027.2
Accession Version	XM_014733027.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	vinculin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014733027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAGTGCCTCC CCAGGGGATG CTGGTGAGCA AGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGC GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCTTGGGAA CCTGCAAAAT GCTAGGACAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GCGCCTCATC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAAGGTCTA 
GATGTGCTCA CAGCGAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGGGATGAC ATTTTACGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TAGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACGGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCTTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTTGCGGCCA CTGCACCCCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAATTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTTTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGATC TGGACAAGTG TAAAGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC CCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAGTTG AGCAAAACTA TCTCCCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAT CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATAGGA TCCTGGGAGC TGTTGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAG CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGAGAGGTC CCTCCCCCCA GACCCCCACC ACCGGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCT GGGGAGGTGA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AACTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT GATTCAATGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGCAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCCACC ATAAGCACCC AGCTGAAAAT CCTGTCCACA 
GTAAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAATCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACT GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014588513.2
CDS	127..3327
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASSVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_014733027.2
Organism	Equus caballus(horse)
Definition	Equus caballus vinculin (VCL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733027.2

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAGTGCCTCC CCAGGGGATG CTGGTGAGCA AGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGC GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCTTGGGAA CCTGCAAAAT GCTAGGACAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GCGCCTCATC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAAGGTCTA 
GATGTGCTCA CAGCGAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGGGATGAC ATTTTACGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TAGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACGGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCTTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTTGCGGCCA CTGCACCCCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAATTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTTTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGATC TGGACAAGTG TAAAGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC CCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAGTTG AGCAAAACTA TCTCCCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAT CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATAGGA TCCTGGGAGC TGTTGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAG CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGAGAGGTC CCTCCCCCCA GACCCCCACC ACCGGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCT GGGGAGGTGA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AACTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT GATTCAATGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGCAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCCACC ATAAGCACCC AGCTGAAAAT CCTGTCCACA 
GTAAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAATCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACT GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_014733025.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014588511.1 Equus caballus vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb09770
Clone ID Related Accession (Same CDS sequence)	XM_014733025.1
Accession Version	XM_014733025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAGTGCCTCC CCAGGGGATG CTGGTGAGCA AGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGC GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCTTGGGAA CCTGCAAAAT GCTAGGACAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GCGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAAGGTCTA 
GATGTGCTCA CAGCGAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGGGATGAC ATTTTACGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TAGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACGGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCTTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTTGCGGCCA CTGCACCCCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAATTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTTTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGATC TGGACAAGTG TAAAGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC CCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAGTTG AGCAAAACTA TCTCCCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAT CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATAGGA TCCTGGGAGC TGTTGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAG CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGAGAGGTC CCTCCCCCCA GACCCCCACC ACCGGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCT GGGGAGGTGA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AACTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTGAGGTGG GTCTAAGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGTT GATGGCTGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA ATGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GCATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC CACCATAAGC 
ACCCAGCTGA AAATCCTGTC CACAGTAAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAAT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACTGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014588511.1
CDS	144..3548
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAEVGLSVVAEADAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_014733025.1
Organism	Equus caballus(horse)
Definition	Equus caballus vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733025.1

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAGTGCCTCC CCAGGGGATG CTGGTGAGCA AGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGC GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCTTGGGAA CCTGCAAAAT GCTAGGACAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GCGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAAGGTCTA 
GATGTGCTCA CAGCGAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGGGATGAC ATTTTACGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TAGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACGGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCTTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTTGCGGCCA CTGCACCCCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAATTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTTTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGATC TGGACAAGTG TAAAGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC CCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAGTTG AGCAAAACTA TCTCCCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAT CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATAGGA TCCTGGGAGC TGTTGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAG CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGAGAGGTC CCTCCCCCCA GACCCCCACC ACCGGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCT GGGGAGGTGA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AACTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTGAGGTGG GTCTAAGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGTT GATGGCTGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA ATGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GCATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC CACCATAAGC 
ACCCAGCTGA AAATCCTGTC CACAGTAAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAAT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACTGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_014733026.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014588512.1 Equus caballus vinculin (VCL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHb09771
Clone ID Related Accession (Same CDS sequence)	XM_014733026.1
Accession Version	XM_014733026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	vinculin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTCCGTGA CCCCAGTGCC TCCCCAGGGG ATGCTGGTGA GCAAGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGCGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCTTGG GAACCTGCAA AATGCTAGGA CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGCGCCTC ACCAGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAAGGT 
CTAGATGTGC TCACAGCGAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGGGAT GACATTTTAC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC CCCAGAGGCC 
CGAGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTCCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATAGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACGG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAGCT CCAAGACTCT 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTTGCGG CCACTGCACC CCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGA GCAGCTAATT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCAGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CGTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTCGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC 
AAATCTTTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ATCTGGACAA GTGTAAAGTA 
GCCATGGCCA ATATTCAGCC TCAGATGCTG GTCGCTGGGG CAACCAGCAT TGCCCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAG TTGAGCAAAA CTATCTCCCC AATGGTAATG 
GATGCAAAGG CTGTGGCTGG AAACATTTCT GATCCTGGCC TGCAAAAGAG CTTCCTGGAC 
TCAGGATATA GGATCCTGGG AGCTGTTGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAGCAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGAGAG GTCCCTCCCC CCAGACCCCC ACCACCGGAG 
GAGAAGGACG AAGAGTTCCC TGAGCAGAAA GCTGGGGAGG TGATTAATCA GCCAATGATG 
ATGGCTGCCA GGCAACTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGTTG ATGGCTGAGA TGTCTCGGCT GGTAAGAGGG 
GGCAGTGGTA CCAAGCGGGC ACTGATTCAA TGTGCCAAGG ACATCGCCAA GGCCTCAGAT 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG CATTAGAACC 
AACCTCTTAC AGGTATGTGA GCGAATCCCC ACCATAAGCA CCCAGCTGAA AATCCTGTCC 
ACAGTAAAGG CCACCATGCT GGGCCGGACC AACATCAGTG ATGAGGAATC TGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTCGA ACTGATGCTG GATTTACACT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014588512.1
CDS	142..3345
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_014733026.1
Organism	Equus caballus(horse)
Definition	Equus caballus vinculin (VCL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733026.1

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTCCGTGA CCCCAGTGCC TCCCCAGGGG ATGCTGGTGA GCAAGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGCGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCTTGG GAACCTGCAA AATGCTAGGA CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGCGCCTC ACCAGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAAGGT 
CTAGATGTGC TCACAGCGAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGGGAT GACATTTTAC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC CCCAGAGGCC 
CGAGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTCCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATAGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACGG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAGCT CCAAGACTCT 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTTGCGG CCACTGCACC CCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGA GCAGCTAATT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCAGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CGTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTCGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC 
AAATCTTTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ATCTGGACAA GTGTAAAGTA 
GCCATGGCCA ATATTCAGCC TCAGATGCTG GTCGCTGGGG CAACCAGCAT TGCCCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAG TTGAGCAAAA CTATCTCCCC AATGGTAATG 
GATGCAAAGG CTGTGGCTGG AAACATTTCT GATCCTGGCC TGCAAAAGAG CTTCCTGGAC 
TCAGGATATA GGATCCTGGG AGCTGTTGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAGCAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGAGAG GTCCCTCCCC CCAGACCCCC ACCACCGGAG 
GAGAAGGACG AAGAGTTCCC TGAGCAGAAA GCTGGGGAGG TGATTAATCA GCCAATGATG 
ATGGCTGCCA GGCAACTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGTTG ATGGCTGAGA TGTCTCGGCT GGTAAGAGGG 
GGCAGTGGTA CCAAGCGGGC ACTGATTCAA TGTGCCAAGG ACATCGCCAA GGCCTCAGAT 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG CATTAGAACC 
AACCTCTTAC AGGTATGTGA GCGAATCCCC ACCATAAGCA CCCAGCTGAA AATCCTGTCC 
ACAGTAAAGG CCACCATGCT GGGCCGGACC AACATCAGTG ATGAGGAATC TGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTCGA ACTGATGCTG GATTTACACT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_014733027.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014588513.1 Equus caballus vinculin (VCL), transcript variant X4, mRNA.

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CloneID	OHb09772
Clone ID Related Accession (Same CDS sequence)	XM_014733027.1
Accession Version	XM_014733027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	vinculin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAGTGCCTCC CCAGGGGATG CTGGTGAGCA AGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGC GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCTTGGGAA CCTGCAAAAT GCTAGGACAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GCGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAAGGTCTA 
GATGTGCTCA CAGCGAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGGGATGAC ATTTTACGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TAGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACGGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCTTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTTGCGGCCA CTGCACCCCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAATTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTTTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGATC TGGACAAGTG TAAAGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC CCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAGTTG AGCAAAACTA TCTCCCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAT CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATAGGA TCCTGGGAGC TGTTGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAG CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGAGAGGTC CCTCCCCCCA GACCCCCACC ACCGGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCT GGGGAGGTGA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AACTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT GATTCAATGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGCAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCCACC ATAAGCACCC AGCTGAAAAT CCTGTCCACA 
GTAAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAATCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACT GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014588513.1
CDS	141..3341
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_014733027.1
Organism	Equus caballus(horse)
Definition	Equus caballus vinculin (VCL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733027.1

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTA AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTAACTC ACCAGGAGCA CCGAGTGATG CTGGTGAACT CAATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AGGAAGCTTT GAAGAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAGTGCCTCC CCAGGGGATG CTGGTGAGCA AGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGC GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCTTGGGAA CCTGCAAAAT GCTAGGACAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GCGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAAGGTCTA 
GATGTGCTCA CAGCGAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGGGATGAC ATTTTACGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TAGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACGGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCTTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTTGCGGCCA CTGCACCCCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAATTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC AGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTTTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGATC TGGACAAGTG TAAAGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC CCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAGTTG AGCAAAACTA TCTCCCCAAT GGTAATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAT CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATAGGA TCCTGGGAGC TGTTGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAG CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGAGAGGTC CCTCCCCCCA GACCCCCACC ACCGGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCT GGGGAGGTGA TTAATCAGCC AATGATGATG 
GCTGCCAGGC AACTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT GATTCAATGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGCAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCCACC ATAAGCACCC AGCTGAAAAT CCTGTCCACA 
GTAAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAATCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACT GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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VCL cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム