IFNG cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	IFNG
Entrez Gene ID	100034181
Full Name	interferon gamma
General protein information	Preferred Names interferon gamma Names interferon gamma IFN-gamma
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081949.1	NP_001075418.1	interferon gamma precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Annotation of the Protein Coding Regions of the Equine Genome.
Hestand MS, Kalbfleisch TS, Coleman SJ, Zeng Z, Liu J, Orlando L, MacLeod JN
PloS one10(6)e0124375(2015)

Role of tumor necrosis factor-?, interferon-? and Fas-ligand on in vitro nitric oxide activity in the corpus luteum.
Galv?o AM, Sz?stek AZ, Skarzynski DJ, Ferreira-Dias GM
Cytokine64(1)18-21(2013 Oct)

The promoter region of interferon-gamma is hypermethylated in neonatal foals and its demethylation is associated with increased gene expression.
Sun L, Gong Z, Oberst EJ, Betancourt A, Adams AA, Horohov DW
Developmental and comparative immunology39(3)273-8(2013 Mar)

Cytokines tumor necrosis factor-? and interferon-? participate in modulation of the equine corpus luteum as autocrine and paracrine factors.
Galv?o A, Skarzynski DJ, Sz?stek A, Silva E, Tramontano A, Mollo A, Mateus L, Ferreira-Dias G
Journal of reproductive immunology93(1)28-37(2012 Jan)

Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory TR1 cell activation and a delay of the Th2 cell response in equine neonates and foals.
Wagner B, Burton A, Ainsworth D
Veterinary research41(4)47(2010 Jul-Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that in equine corpus luteum, cytokines TNF, IFNG and FASL regulate nitric oxide activity, via eNOS expression modulation.
Title: Role of tumor necrosis factor-?, interferon-? and Fas-ligand on in vitro nitric oxide activity in the corpus luteum.

IFN-gamma expression in foals appears to be controlled by DNA methylation in the promoter region of Ifng. The age-associated demethylation of the DNA in foals may be induced by exposure to environmental antigens and their effect on lymphoproliferation.
Title: The promoter region of interferon-gamma is hypermethylated in neonatal foals and its demethylation is associated with increased gene expression.

These data show the presence of cytokines TNF and IFNG, and their receptors, in the equine corpus luteum and indicate their potential involvement in regulation of luteal function.
Title: Cytokines tumor necrosis factor-? and interferon-? participate in modulation of the equine corpus luteum as autocrine and paracrine factors.

The data suggested in foals there is an impaired Th2 response, the immune response is Th1 biased, interferon-gamma production is qualitatively similar to adult horses, and regulatory IL-10 production by T cells is mature.
Title: Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory TR1 cell activation and a delay of the Th2 cell response in equine neonates and foals.

The following IFNG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IFNG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHb00097	NM_001081949.1 Latest version!	Equus caballus interferon gamma (IFNG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IFNG ( NM_001081949.1 ) cDNA ORF clone, Equus caballus(horse) -> NP_001075418.1 Equus caballus interferon gamma (IFNG), mRNA.

Price & Availability↑

CloneID	OHb00097
Clone ID Related Accession (Same CDS sequence)	NM_001081949.1
Accession Version	NM_001081949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Equus caballus(horse)
Product	interferon gamma precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U04050.1. ##Evidence-Data-START## Transcript exon combination :: U04050.1, DN505413.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2743238, SAMN00991836 [ECO:0000348] ##Evidence-Data-END##

ATGAATTATA CAAGTTTTAT CTTGGCTTTT CAGCTGTGTG CGATTTTGGG TTCTTCTACC 
TATTACTGCC AGGCCGCGTT TTTTAAAGAA ATAGAAAACC TAAAGGAATA TTTTAACGCA 
AGTAACCCAG ATGTAGGGGA TGGTGGGCCT CTTTTCCTGG ATATCTTGAA GAACTGGAAA 
GAGGATAGTG ACAAAAAAAT AATTCAGAGC CAAATCGTCT CCTTCTACTT CAAACTCTTT 
GAAAACTTGA AAGATAACCA GGTCATTCAA AAGAGCATGG ACACCATCAA GGAGGACCTG 
TTCGTTAAGT TCTTTAACAG CAGCACCAGC AAGCTGGAAG ACTTCCAAAA GCTGATTCAG 
ATTCCGGTAA ATGATCTGAA GGTCCAGCGC AAAGCAATAA GTGAACTCAT CAAAGTGATG 
AATGATCTGT CGCCCAAAGC TAACCTGAGG AAGCGGAAGA GGAGTCAGAA TCCATTTCGA 
GGCCGGAGAG CGTTGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075418.1
CDS	65..565
Translation	MNYTSFILAFQLCAILGSSTYYCQAAFFKEIENLKEYFNASNPDVGDGGPLFLDILKNWKEDSDKKIIQSQIVSFYFKLFENLKDNQVIQKSMDTIKEDLFVKFFNSSTSKLEDFQKLIQIPVNDLKVQRKAISELIKVMNDLSPKANLRKRKRSQNPFRGRRALQ

Target ORF information:

RefSeq Version	NM_001081949.1
Organism	Equus caballus(horse)
Definition	Equus caballus interferon gamma (IFNG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081949.1

ORF Insert Sequence:

ATGAATTATA CAAGTTTTAT CTTGGCTTTT CAGCTGTGTG CGATTTTGGG TTCTTCTACC 
TATTACTGCC AGGCCGCGTT TTTTAAAGAA ATAGAAAACC TAAAGGAATA TTTTAACGCA 
AGTAACCCAG ATGTAGGGGA TGGTGGGCCT CTTTTCCTGG ATATCTTGAA GAACTGGAAA 
GAGGATAGTG ACAAAAAAAT AATTCAGAGC CAAATCGTCT CCTTCTACTT CAAACTCTTT 
GAAAACTTGA AAGATAACCA GGTCATTCAA AAGAGCATGG ACACCATCAA GGAGGACCTG 
TTCGTTAAGT TCTTTAACAG CAGCACCAGC AAGCTGGAAG ACTTCCAAAA GCTGATTCAG 
ATTCCGGTAA ATGATCTGAA GGTCCAGCGC AAAGCAATAA GTGAACTCAT CAAAGTGATG 
AATGATCTGT CGCCCAAAGC TAACCTGAGG AAGCGGAAGA GGAGTCAGAA TCCATTTCGA 
GGCCGGAGAG CGTTGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Annotation of the Protein Coding Regions of the Equine Genome.
PloS one10(6)e0124375(2015)
Hestand MS,Kalbfleisch TS,Coleman SJ,Zeng Z,Liu J,Orlando L,MacLeod JN

Role of tumor necrosis factor-?, interferon-? and Fas-ligand on in vitro nitric oxide activity in the corpus luteum.
Cytokine64(1)18-21(2013 Oct)
Galv?o AM,Sz?stek AZ,Skarzynski DJ,Ferreira-Dias GM

The promoter region of interferon-gamma is hypermethylated in neonatal foals and its demethylation is associated with increased gene expression.
Developmental and comparative immunology39(3)273-8(2013 Mar)
Sun L,Gong Z,Oberst EJ,Betancourt A,Adams AA,Horohov DW

Cytokines tumor necrosis factor-? and interferon-? participate in modulation of the equine corpus luteum as autocrine and paracrine factors.
Journal of reproductive immunology93(1)28-37(2012 Jan)
Galv?o A,Skarzynski DJ,Sz?stek A,Silva E,Tramontano A,Mollo A,Mateus L,Ferreira-Dias G

Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory TR1 cell activation and a delay of the Th2 cell response in equine neonates and foals.
Veterinary research41(4)47(2010 Jul-Aug)
Wagner B,Burton A,Ainsworth D