ACLY cDNA ORF clone, Egretta garzetta(little egret)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACLY
Entrez Gene ID	104131387
Full Name	ATP citrate lyase
Gene Type	protein-coding
Organism	Egretta garzetta(little egret)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009644546.1	XP_009642841.1	ATP-citrate synthase isoform X1
XM_009644547.1	XP_009642842.1	ATP-citrate synthase isoform X2

Pathways from BioSystems
Gene Ontology

The following ACLY gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACLY cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEg12099	XM_009644546.1 Latest version!	Egretta garzetta ATP citrate lyase (ACLY), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OEg12100	XM_009644547.1 Latest version!	Egretta garzetta ATP citrate lyase (ACLY), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACLY ( XM_009644546.1 ) cDNA ORF clone, Egretta garzetta(little egret) -> XP_009642841.1 Egretta garzetta ATP citrate lyase (ACLY), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEg12099
Clone ID Related Accession (Same CDS sequence)	XM_009644546.1
Accession Version	XM_009644546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-15
Organism	Egretta garzetta(little egret)
Product	ATP-citrate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009260504.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Egretta garzetta Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA AAGCCATCTC GGAGCAGACG GGGAAGGAGT TCTTGTATAA GTTTATCTGC 
ACATCCTCTG CCATCCAGAA CCGTTTCAAG TATGCCCGAG TCACCCCAGA CACTGACTGG 
GCACGACTCA CCCAGGACCA CCCTTGGCTT CTGAGCGAGC GTTTAGTGGT GAAGCCAGAT 
CAGCTAATAA AGAGACGTGG GAAGCTCGGA CTGGTTGGAA TTAACCTCAC TCTGGATCAG 
GTGAAGGTTT GGCTTAAACA GCGTCTGGGC CAAGAAACCA CGATTGCTAA TGCTAAAGGG 
ATCCTAAAGA ATTTTCTGAT TGAACCCTTC GTCCCTCATA AACAGGAGGA AGAGTTCTAT 
GTGTGCATAT ATGCTGCCCG TGAGGGAGAC TACGTACTCT TCCACCACGA AGGGGGTGTG 
GATGTGGGAG ATGTTGATGC CAAAGCTCAG AAGTTGCTTG TGGCAGTGGA TGAAAAGCTC 
AGTGAATTGG ATGTAAAGAA ACATCTCCTG CAGCATGCAC CAGCAAATAA AAAGGATGTC 
TTGGCTAGCT TCATCTGTGG CCTGTTCAAC CTTTATGAAG ATCTGTATTT CACATACCTC 
GAGATCAATC CGTTAGTGGT GACTAACGAT GGTGTCTACA TCCTTGATTT GGCCGCAAAG 
ATTGATGCGA CTGCTGATTA TATCTGCAAA GTGAAATGGG GGGATGTGGA GTTTCCCCCT 
CCCTTTGGCA GGGAAGCTTA CCCAGAGGAG GCCTACATTG CTGATCTGGA TGCGAAGAGC 
GGAGCCAGCT TGAAGCTTAC TATTCTCAAC CCCAAAGGCA GGATCTGGAC CATGGTGGCT 
GGTGGCGGCG CCTCCGTGGT TTACAGTGAC ACCATTTGTG ACCTGGGGGG TGTGAATGAG 
CTGGCTAACT ATGGGGAATA CTCAGGAGCC CCAAGTGAGC AACAGACCTA TGACTATGCT 
AAAACTATTC TCTCCCTCAT GACACGAGAG AAGCACCCTG AAGGTAAAAT CCTGATCATT 
GGAGGCAGCA TTGCTAACTT CACCAATGTA GCAGCCACTT TTAAAGGCAT TGTGAGAGCG 
ATTAAGGATT ACCAAGGCCC TCTGAAGGAG CATGAGGTGA GGATCTTTGT ACGAAGAGGA 
GGCCCGAACT ACCAGGAAGG ATTACGTGTC ATGGGAGAAG TTGGGAAGAC CACTGGGATC 
CCCATCCATG TTTTTGGCAC GGAGACTCAC ATGACTGCGA TTGTGGGCAT GGCACTCGGC 
CATCGGCCTA TCCCCAACCA ACCGCCTGCT GCAGCCCACA CTGCCAACTT CCTTCTCAAT 
GCCAGCGGCA GCCCCTCGAC TCCAGCACCA AGCAGAACGG CTTCCTTCTC AGAGTCCAAA 
CCAGATGATA TTGCTCCAGC CAAGAAGGCA AAACCAACAG CACCTCTTGA TTCAATCCCA 
GCTTCAAGAC CTGGTTCAGG TAAAGCTACA ACCCTGTTCA GCCGTCACAC CAAAGCTATC 
ATTTGGGGGA TGCAGACACG GGCTGTGCAA GGAATGCTGG ATTTTGATTA TATTTGCTCC 
CGGGATGAAC CTTCAGTAGC TGCCATGGTT TATCCATTCA CTGGTGACCA CAGGCAGAAG 
TTCTATTGGG GCCACAAAGA AATCCTGATC CCAGTCTACA AGAACATGTC AGATGCCATG 
AGGAAGCACC CAGAGGTGGA TGTTCTCATC AACTTTGCAT CTCTGCGCTC TGCTTATGAC 
AGCACTGTTG AAACCATGAA TTATCCACAG ATCCGCACCA TTGCCATTAT TGCCGAGGGC 
ATTCCAGAGG CCCTGACTCG AAAACTGATC AAGACAGCTG ATAAAAAAGG AGTCACTATC 
ATTGGGCCTG CAACTGTTGG TGGGATCAAA CCAGGTTGCT TCAAAATAGG CAACACAGGA 
GGCATGCTGG ATAATATCTT GGCATCAAAA CTGTACCGGC CTGGCAGCGT GGCTTATGTT 
TCACGGTCCG GAGGCATGTC CAATGAGCTC AACAACATCA TTTCCCGAAC CACTGACGGG 
GTCTATGAAG GGGTGGCCAT TGGAGGAGAC AGATACCCTG GTTCAACTTT TATGGATCAT 
GTCTTGCGTT ATGAGGATAC TCCAGGAGTG AAAATGATTG TAGTACTGGG AGAGATTGGA 
GGCACAGAAG AGTATAAGAT CTGCAGGGGC ATTAAAGAAG GTCGTATCAC CAAGCCGGTG 
GTGTGTTGGT GTATTGGTAC TTGTGCCACC ATGTTCTCTT CAGAGGTGCA GTTTGGCCAC 
GCAGGAGCTT GTGCCAACCA AGCTTCTGAA ACTGCTGTTG CAAAGAATCA AGCCTTGAAG 
GAAGCTGGTG TGTTTGTTCC CCGGAGTTTT GACGAGCTGG GAGAGGTCAT TCAGTCTGTC 
TACCAGGATC TTGTGGCCAA AAGAGTGATT GAACCAGCTG AAGAAGTGCC TCCTCCAACT 
GTGCCAATGG ATTACTCCTG GGCAAGGGAG CTGGGTCTGA TCCGCAAACC GGCTTCCTTT 
ATGACAAGCA TTTGTGATGA GAGAGGTCAG GAACTGATTT ATGCTGGGAT GCCAATCACT 
GAGGTCTTCA AAGAAGAGAT GGGGATTGGA GGGGTTCTGG GCCTGCTCTG GTTTCAAAGG 
AGGTTACCAA AGTATGCCTG CCAGTTCATT GAGATGTGTT TGATGGTAAC AGCAGATCAT 
GGGCCTGCTG TTTCTGGAGC CCACAACACC ATTGTCTGTG CAAGAGCTGG AAAAGATCTT 
GTCTCGAGTC TCACTTCAGG CCTTCTTACT ATTGGTGACC GGTTTGGTGG AGCACTGGAT 
GCTGCAGCTA AAATGTTCAG CAAAGCCTTT GACAGTGGAA TTATCCCTAT GGAATTTGTG 
AACAAGATGA AGAAAGAAGG GAAACTGATC ATGGGCATTG GACACAGAGT CAAATCGATA 
AATAACCCAG ACATGAGAGT TCAGATTCTC AAAGACTACG TGAAGCAGCA TTTCCCTGCC 
ACCCCACTGT TGGACTATGC ACTTGAAGTA GAAAAAATTA CGACTTCCAA GAAACCAAAT 
CTTATCCTGA ATGTAGATGG CTTTATTGGA GTTGCCTTTG TTGACGTACT CAGGAATTGT 
GGCTCGTTCA CACGGGAAGA AGCAGATGAG TATATTGATA TAGGAGCTCT TAATGGCATC 
TTTGTACTAG GCAGGAGTAT GGGATTCATT GGACACTACC TGGATCAGAA GAGATTGAAG 
CAAGGCCTGT ACCGTCACCC GTGGGATGAC ATATCCTATG TTTTACCAGA GCACATGACT 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009642841.1
CDS	17..3322
Translation	MSAKAISEQTGKEFLYKFICTSSAIQNRFKYARVTPDTDWARLTQDHPWLLSERLVVKPDQLIKRRGKLGLVGINLTLDQVKVWLKQRLGQETTIANAKGILKNFLIEPFVPHKQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDAKAQKLLVAVDEKLSELDVKKHLLQHAPANKKDVLASFICGLFNLYEDLYFTYLEINPLVVTNDGVYILDLAAKIDATADYICKVKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPEGKILIIGGSIANFTNVAATFKGIVRAIKDYQGPLKEHEVRIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPAAAHTANFLLNASGSPSTPAPSRTASFSESKPDDIAPAKKAKPTAPLDSIPASRPGSGKATTLFSRHTKAIIWGMQTRAVQGMLDFDYICSRDEPSVAAMVYPFTGDHRQKFYWGHKEILIPVYKNMSDAMRKHPEVDVLINFASLRSAYDSTVETMNYPQIRTIAIIAEGIPEALTRKLIKTADKKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYEDTPGVKMIVVLGEIGGTEEYKICRGIKEGRITKPVVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEVIQSVYQDLVAKRVIEPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQRRLPKYACQFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQHFPATPLLDYALEVEKITTSKKPNLILNVDGFIGVAFVDVLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	XM_009644546.1
Organism	Egretta garzetta(little egret)
Definition	Egretta garzetta ATP citrate lyase (ACLY), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009644546.1

ORF Insert Sequence:

ATGTCAGCCA AAGCCATCTC GGAGCAGACG GGGAAGGAGT TCTTGTATAA GTTTATCTGC 
ACATCCTCTG CCATCCAGAA CCGTTTCAAG TATGCCCGAG TCACCCCAGA CACTGACTGG 
GCACGACTCA CCCAGGACCA CCCTTGGCTT CTGAGCGAGC GTTTAGTGGT GAAGCCAGAT 
CAGCTAATAA AGAGACGTGG GAAGCTCGGA CTGGTTGGAA TTAACCTCAC TCTGGATCAG 
GTGAAGGTTT GGCTTAAACA GCGTCTGGGC CAAGAAACCA CGATTGCTAA TGCTAAAGGG 
ATCCTAAAGA ATTTTCTGAT TGAACCCTTC GTCCCTCATA AACAGGAGGA AGAGTTCTAT 
GTGTGCATAT ATGCTGCCCG TGAGGGAGAC TACGTACTCT TCCACCACGA AGGGGGTGTG 
GATGTGGGAG ATGTTGATGC CAAAGCTCAG AAGTTGCTTG TGGCAGTGGA TGAAAAGCTC 
AGTGAATTGG ATGTAAAGAA ACATCTCCTG CAGCATGCAC CAGCAAATAA AAAGGATGTC 
TTGGCTAGCT TCATCTGTGG CCTGTTCAAC CTTTATGAAG ATCTGTATTT CACATACCTC 
GAGATCAATC CGTTAGTGGT GACTAACGAT GGTGTCTACA TCCTTGATTT GGCCGCAAAG 
ATTGATGCGA CTGCTGATTA TATCTGCAAA GTGAAATGGG GGGATGTGGA GTTTCCCCCT 
CCCTTTGGCA GGGAAGCTTA CCCAGAGGAG GCCTACATTG CTGATCTGGA TGCGAAGAGC 
GGAGCCAGCT TGAAGCTTAC TATTCTCAAC CCCAAAGGCA GGATCTGGAC CATGGTGGCT 
GGTGGCGGCG CCTCCGTGGT TTACAGTGAC ACCATTTGTG ACCTGGGGGG TGTGAATGAG 
CTGGCTAACT ATGGGGAATA CTCAGGAGCC CCAAGTGAGC AACAGACCTA TGACTATGCT 
AAAACTATTC TCTCCCTCAT GACACGAGAG AAGCACCCTG AAGGTAAAAT CCTGATCATT 
GGAGGCAGCA TTGCTAACTT CACCAATGTA GCAGCCACTT TTAAAGGCAT TGTGAGAGCG 
ATTAAGGATT ACCAAGGCCC TCTGAAGGAG CATGAGGTGA GGATCTTTGT ACGAAGAGGA 
GGCCCGAACT ACCAGGAAGG ATTACGTGTC ATGGGAGAAG TTGGGAAGAC CACTGGGATC 
CCCATCCATG TTTTTGGCAC GGAGACTCAC ATGACTGCGA TTGTGGGCAT GGCACTCGGC 
CATCGGCCTA TCCCCAACCA ACCGCCTGCT GCAGCCCACA CTGCCAACTT CCTTCTCAAT 
GCCAGCGGCA GCCCCTCGAC TCCAGCACCA AGCAGAACGG CTTCCTTCTC AGAGTCCAAA 
CCAGATGATA TTGCTCCAGC CAAGAAGGCA AAACCAACAG CACCTCTTGA TTCAATCCCA 
GCTTCAAGAC CTGGTTCAGG TAAAGCTACA ACCCTGTTCA GCCGTCACAC CAAAGCTATC 
ATTTGGGGGA TGCAGACACG GGCTGTGCAA GGAATGCTGG ATTTTGATTA TATTTGCTCC 
CGGGATGAAC CTTCAGTAGC TGCCATGGTT TATCCATTCA CTGGTGACCA CAGGCAGAAG 
TTCTATTGGG GCCACAAAGA AATCCTGATC CCAGTCTACA AGAACATGTC AGATGCCATG 
AGGAAGCACC CAGAGGTGGA TGTTCTCATC AACTTTGCAT CTCTGCGCTC TGCTTATGAC 
AGCACTGTTG AAACCATGAA TTATCCACAG ATCCGCACCA TTGCCATTAT TGCCGAGGGC 
ATTCCAGAGG CCCTGACTCG AAAACTGATC AAGACAGCTG ATAAAAAAGG AGTCACTATC 
ATTGGGCCTG CAACTGTTGG TGGGATCAAA CCAGGTTGCT TCAAAATAGG CAACACAGGA 
GGCATGCTGG ATAATATCTT GGCATCAAAA CTGTACCGGC CTGGCAGCGT GGCTTATGTT 
TCACGGTCCG GAGGCATGTC CAATGAGCTC AACAACATCA TTTCCCGAAC CACTGACGGG 
GTCTATGAAG GGGTGGCCAT TGGAGGAGAC AGATACCCTG GTTCAACTTT TATGGATCAT 
GTCTTGCGTT ATGAGGATAC TCCAGGAGTG AAAATGATTG TAGTACTGGG AGAGATTGGA 
GGCACAGAAG AGTATAAGAT CTGCAGGGGC ATTAAAGAAG GTCGTATCAC CAAGCCGGTG 
GTGTGTTGGT GTATTGGTAC TTGTGCCACC ATGTTCTCTT CAGAGGTGCA GTTTGGCCAC 
GCAGGAGCTT GTGCCAACCA AGCTTCTGAA ACTGCTGTTG CAAAGAATCA AGCCTTGAAG 
GAAGCTGGTG TGTTTGTTCC CCGGAGTTTT GACGAGCTGG GAGAGGTCAT TCAGTCTGTC 
TACCAGGATC TTGTGGCCAA AAGAGTGATT GAACCAGCTG AAGAAGTGCC TCCTCCAACT 
GTGCCAATGG ATTACTCCTG GGCAAGGGAG CTGGGTCTGA TCCGCAAACC GGCTTCCTTT 
ATGACAAGCA TTTGTGATGA GAGAGGTCAG GAACTGATTT ATGCTGGGAT GCCAATCACT 
GAGGTCTTCA AAGAAGAGAT GGGGATTGGA GGGGTTCTGG GCCTGCTCTG GTTTCAAAGG 
AGGTTACCAA AGTATGCCTG CCAGTTCATT GAGATGTGTT TGATGGTAAC AGCAGATCAT 
GGGCCTGCTG TTTCTGGAGC CCACAACACC ATTGTCTGTG CAAGAGCTGG AAAAGATCTT 
GTCTCGAGTC TCACTTCAGG CCTTCTTACT ATTGGTGACC GGTTTGGTGG AGCACTGGAT 
GCTGCAGCTA AAATGTTCAG CAAAGCCTTT GACAGTGGAA TTATCCCTAT GGAATTTGTG 
AACAAGATGA AGAAAGAAGG GAAACTGATC ATGGGCATTG GACACAGAGT CAAATCGATA 
AATAACCCAG ACATGAGAGT TCAGATTCTC AAAGACTACG TGAAGCAGCA TTTCCCTGCC 
ACCCCACTGT TGGACTATGC ACTTGAAGTA GAAAAAATTA CGACTTCCAA GAAACCAAAT 
CTTATCCTGA ATGTAGATGG CTTTATTGGA GTTGCCTTTG TTGACGTACT CAGGAATTGT 
GGCTCGTTCA CACGGGAAGA AGCAGATGAG TATATTGATA TAGGAGCTCT TAATGGCATC 
TTTGTACTAG GCAGGAGTAT GGGATTCATT GGACACTACC TGGATCAGAA GAGATTGAAG 
CAAGGCCTGT ACCGTCACCC GTGGGATGAC ATATCCTATG TTTTACCAGA GCACATGACT 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACLY ( XM_009644547.1 ) cDNA ORF clone, Egretta garzetta(little egret) -> XP_009642842.1 Egretta garzetta ATP citrate lyase (ACLY), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEg12100
Clone ID Related Accession (Same CDS sequence)	XM_009644547.1
Accession Version	XM_009644547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-15
Organism	Egretta garzetta(little egret)
Product	ATP-citrate synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009260504.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Egretta garzetta Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA AAGCCATCTC GGAGCAGACG GGGAAGGAGT TCTTGTATAA GTTTATCTGC 
ACATCCTCTG CCATCCAGAA CCGTTTCAAG TATGCCCGAG TCACCCCAGA CACTGACTGG 
GCACGACTCA CCCAGGACCA CCCTTGGCTT CTGAGCGAGC GTTTAGTGGT GAAGCCAGAT 
CAGCTAATAA AGAGACGTGG GAAGCTCGGA CTGGTTGGAA TTAACCTCAC TCTGGATCAG 
GTGAAGGTTT GGCTTAAACA GCGTCTGGGC CAAGAAACCA CGATTGCTAA TGCTAAAGGG 
ATCCTAAAGA ATTTTCTGAT TGAACCCTTC GTCCCTCATA AACAGGAGGA AGAGTTCTAT 
GTGTGCATAT ATGCTGCCCG TGAGGGAGAC TACGTACTCT TCCACCACGA AGGGGGTGTG 
GATGTGGGAG ATGTTGATGC CAAAGCTCAG AAGTTGCTTG TGGCAGTGGA TGAAAAGCTC 
AGTGAATTGG ATGTAAAGAA ACATCTCCTG CAGCATGCAC CAGCAAATAA AAAGGATGTC 
TTGGCTAGCT TCATCTGTGG CCTGTTCAAC CTTTATGAAG ATCTGTATTT CACATACCTC 
GAGATCAATC CGTTAGTGGT GACTAACGAT GGTGTCTACA TCCTTGATTT GGCCGCAAAG 
ATTGATGCGA CTGCTGATTA TATCTGCAAA GTGAAATGGG GGGATGTGGA GTTTCCCCCT 
CCCTTTGGCA GGGAAGCTTA CCCAGAGGAG GCCTACATTG CTGATCTGGA TGCGAAGAGC 
GGAGCCAGCT TGAAGCTTAC TATTCTCAAC CCCAAAGGCA GGATCTGGAC CATGGTGGCT 
GGTGGCGGCG CCTCCGTGGT TTACAGTGAC ACCATTTGTG ACCTGGGGGG TGTGAATGAG 
CTGGCTAACT ATGGGGAATA CTCAGGAGCC CCAAGTGAGC AACAGACCTA TGACTATGCT 
AAAACTATTC TCTCCCTCAT GACACGAGAG AAGCACCCTG AAGGTAAAAT CCTGATCATT 
GGAGGCAGCA TTGCTAACTT CACCAATGTA GCAGCCACTT TTAAAGGCAT TGTGAGAGCG 
ATTAAGGATT ACCAAGGCCC TCTGAAGGAG CATGAGGTGA GGATCTTTGT ACGAAGAGGA 
GGCCCGAACT ACCAGGAAGG ATTACGTGTC ATGGGAGAAG TTGGGAAGAC CACTGGGATC 
CCCATCCATG TTTTTGGCAC GGAGACTCAC ATGACTGCGA TTGTGGGCAT GGCACTCGGC 
CATCGGCCTA TCCCCAACCA ACCGCCTGCT GCAGCCCACA CTGCCAACTT CCTTCTCAAT 
GCCAGCGGCA GCCCCTCGAC TCCAGCACCA AGCAGAACGG CTTCCTTCTC AGAGTCCAAA 
CCAGATGATA TTGCTCCAGC CAAGAAGGCA AAACCAACAG CACCTCTTGG TAAAGCTACA 
ACCCTGTTCA GCCGTCACAC CAAAGCTATC ATTTGGGGGA TGCAGACACG GGCTGTGCAA 
GGAATGCTGG ATTTTGATTA TATTTGCTCC CGGGATGAAC CTTCAGTAGC TGCCATGGTT 
TATCCATTCA CTGGTGACCA CAGGCAGAAG TTCTATTGGG GCCACAAAGA AATCCTGATC 
CCAGTCTACA AGAACATGTC AGATGCCATG AGGAAGCACC CAGAGGTGGA TGTTCTCATC 
AACTTTGCAT CTCTGCGCTC TGCTTATGAC AGCACTGTTG AAACCATGAA TTATCCACAG 
ATCCGCACCA TTGCCATTAT TGCCGAGGGC ATTCCAGAGG CCCTGACTCG AAAACTGATC 
AAGACAGCTG ATAAAAAAGG AGTCACTATC ATTGGGCCTG CAACTGTTGG TGGGATCAAA 
CCAGGTTGCT TCAAAATAGG CAACACAGGA GGCATGCTGG ATAATATCTT GGCATCAAAA 
CTGTACCGGC CTGGCAGCGT GGCTTATGTT TCACGGTCCG GAGGCATGTC CAATGAGCTC 
AACAACATCA TTTCCCGAAC CACTGACGGG GTCTATGAAG GGGTGGCCAT TGGAGGAGAC 
AGATACCCTG GTTCAACTTT TATGGATCAT GTCTTGCGTT ATGAGGATAC TCCAGGAGTG 
AAAATGATTG TAGTACTGGG AGAGATTGGA GGCACAGAAG AGTATAAGAT CTGCAGGGGC 
ATTAAAGAAG GTCGTATCAC CAAGCCGGTG GTGTGTTGGT GTATTGGTAC TTGTGCCACC 
ATGTTCTCTT CAGAGGTGCA GTTTGGCCAC GCAGGAGCTT GTGCCAACCA AGCTTCTGAA 
ACTGCTGTTG CAAAGAATCA AGCCTTGAAG GAAGCTGGTG TGTTTGTTCC CCGGAGTTTT 
GACGAGCTGG GAGAGGTCAT TCAGTCTGTC TACCAGGATC TTGTGGCCAA AAGAGTGATT 
GAACCAGCTG AAGAAGTGCC TCCTCCAACT GTGCCAATGG ATTACTCCTG GGCAAGGGAG 
CTGGGTCTGA TCCGCAAACC GGCTTCCTTT ATGACAAGCA TTTGTGATGA GAGAGGTCAG 
GAACTGATTT ATGCTGGGAT GCCAATCACT GAGGTCTTCA AAGAAGAGAT GGGGATTGGA 
GGGGTTCTGG GCCTGCTCTG GTTTCAAAGG AGGTTACCAA AGTATGCCTG CCAGTTCATT 
GAGATGTGTT TGATGGTAAC AGCAGATCAT GGGCCTGCTG TTTCTGGAGC CCACAACACC 
ATTGTCTGTG CAAGAGCTGG AAAAGATCTT GTCTCGAGTC TCACTTCAGG CCTTCTTACT 
ATTGGTGACC GGTTTGGTGG AGCACTGGAT GCTGCAGCTA AAATGTTCAG CAAAGCCTTT 
GACAGTGGAA TTATCCCTAT GGAATTTGTG AACAAGATGA AGAAAGAAGG GAAACTGATC 
ATGGGCATTG GACACAGAGT CAAATCGATA AATAACCCAG ACATGAGAGT TCAGATTCTC 
AAAGACTACG TGAAGCAGCA TTTCCCTGCC ACCCCACTGT TGGACTATGC ACTTGAAGTA 
GAAAAAATTA CGACTTCCAA GAAACCAAAT CTTATCCTGA ATGTAGATGG CTTTATTGGA 
GTTGCCTTTG TTGACGTACT CAGGAATTGT GGCTCGTTCA CACGGGAAGA AGCAGATGAG 
TATATTGATA TAGGAGCTCT TAATGGCATC TTTGTACTAG GCAGGAGTAT GGGATTCATT 
GGACACTACC TGGATCAGAA GAGATTGAAG CAAGGCCTGT ACCGTCACCC GTGGGATGAC 
ATATCCTATG TTTTACCAGA GCACATGACT ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009642842.1
CDS	17..3292
Translation	MSAKAISEQTGKEFLYKFICTSSAIQNRFKYARVTPDTDWARLTQDHPWLLSERLVVKPDQLIKRRGKLGLVGINLTLDQVKVWLKQRLGQETTIANAKGILKNFLIEPFVPHKQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDAKAQKLLVAVDEKLSELDVKKHLLQHAPANKKDVLASFICGLFNLYEDLYFTYLEINPLVVTNDGVYILDLAAKIDATADYICKVKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPEGKILIIGGSIANFTNVAATFKGIVRAIKDYQGPLKEHEVRIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPAAAHTANFLLNASGSPSTPAPSRTASFSESKPDDIAPAKKAKPTAPLGKATTLFSRHTKAIIWGMQTRAVQGMLDFDYICSRDEPSVAAMVYPFTGDHRQKFYWGHKEILIPVYKNMSDAMRKHPEVDVLINFASLRSAYDSTVETMNYPQIRTIAIIAEGIPEALTRKLIKTADKKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYEDTPGVKMIVVLGEIGGTEEYKICRGIKEGRITKPVVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEVIQSVYQDLVAKRVIEPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQRRLPKYACQFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQHFPATPLLDYALEVEKITTSKKPNLILNVDGFIGVAFVDVLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	XM_009644547.1
Organism	Egretta garzetta(little egret)
Definition	Egretta garzetta ATP citrate lyase (ACLY), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009644547.1

ORF Insert Sequence:

ATGTCAGCCA AAGCCATCTC GGAGCAGACG GGGAAGGAGT TCTTGTATAA GTTTATCTGC 
ACATCCTCTG CCATCCAGAA CCGTTTCAAG TATGCCCGAG TCACCCCAGA CACTGACTGG 
GCACGACTCA CCCAGGACCA CCCTTGGCTT CTGAGCGAGC GTTTAGTGGT GAAGCCAGAT 
CAGCTAATAA AGAGACGTGG GAAGCTCGGA CTGGTTGGAA TTAACCTCAC TCTGGATCAG 
GTGAAGGTTT GGCTTAAACA GCGTCTGGGC CAAGAAACCA CGATTGCTAA TGCTAAAGGG 
ATCCTAAAGA ATTTTCTGAT TGAACCCTTC GTCCCTCATA AACAGGAGGA AGAGTTCTAT 
GTGTGCATAT ATGCTGCCCG TGAGGGAGAC TACGTACTCT TCCACCACGA AGGGGGTGTG 
GATGTGGGAG ATGTTGATGC CAAAGCTCAG AAGTTGCTTG TGGCAGTGGA TGAAAAGCTC 
AGTGAATTGG ATGTAAAGAA ACATCTCCTG CAGCATGCAC CAGCAAATAA AAAGGATGTC 
TTGGCTAGCT TCATCTGTGG CCTGTTCAAC CTTTATGAAG ATCTGTATTT CACATACCTC 
GAGATCAATC CGTTAGTGGT GACTAACGAT GGTGTCTACA TCCTTGATTT GGCCGCAAAG 
ATTGATGCGA CTGCTGATTA TATCTGCAAA GTGAAATGGG GGGATGTGGA GTTTCCCCCT 
CCCTTTGGCA GGGAAGCTTA CCCAGAGGAG GCCTACATTG CTGATCTGGA TGCGAAGAGC 
GGAGCCAGCT TGAAGCTTAC TATTCTCAAC CCCAAAGGCA GGATCTGGAC CATGGTGGCT 
GGTGGCGGCG CCTCCGTGGT TTACAGTGAC ACCATTTGTG ACCTGGGGGG TGTGAATGAG 
CTGGCTAACT ATGGGGAATA CTCAGGAGCC CCAAGTGAGC AACAGACCTA TGACTATGCT 
AAAACTATTC TCTCCCTCAT GACACGAGAG AAGCACCCTG AAGGTAAAAT CCTGATCATT 
GGAGGCAGCA TTGCTAACTT CACCAATGTA GCAGCCACTT TTAAAGGCAT TGTGAGAGCG 
ATTAAGGATT ACCAAGGCCC TCTGAAGGAG CATGAGGTGA GGATCTTTGT ACGAAGAGGA 
GGCCCGAACT ACCAGGAAGG ATTACGTGTC ATGGGAGAAG TTGGGAAGAC CACTGGGATC 
CCCATCCATG TTTTTGGCAC GGAGACTCAC ATGACTGCGA TTGTGGGCAT GGCACTCGGC 
CATCGGCCTA TCCCCAACCA ACCGCCTGCT GCAGCCCACA CTGCCAACTT CCTTCTCAAT 
GCCAGCGGCA GCCCCTCGAC TCCAGCACCA AGCAGAACGG CTTCCTTCTC AGAGTCCAAA 
CCAGATGATA TTGCTCCAGC CAAGAAGGCA AAACCAACAG CACCTCTTGG TAAAGCTACA 
ACCCTGTTCA GCCGTCACAC CAAAGCTATC ATTTGGGGGA TGCAGACACG GGCTGTGCAA 
GGAATGCTGG ATTTTGATTA TATTTGCTCC CGGGATGAAC CTTCAGTAGC TGCCATGGTT 
TATCCATTCA CTGGTGACCA CAGGCAGAAG TTCTATTGGG GCCACAAAGA AATCCTGATC 
CCAGTCTACA AGAACATGTC AGATGCCATG AGGAAGCACC CAGAGGTGGA TGTTCTCATC 
AACTTTGCAT CTCTGCGCTC TGCTTATGAC AGCACTGTTG AAACCATGAA TTATCCACAG 
ATCCGCACCA TTGCCATTAT TGCCGAGGGC ATTCCAGAGG CCCTGACTCG AAAACTGATC 
AAGACAGCTG ATAAAAAAGG AGTCACTATC ATTGGGCCTG CAACTGTTGG TGGGATCAAA 
CCAGGTTGCT TCAAAATAGG CAACACAGGA GGCATGCTGG ATAATATCTT GGCATCAAAA 
CTGTACCGGC CTGGCAGCGT GGCTTATGTT TCACGGTCCG GAGGCATGTC CAATGAGCTC 
AACAACATCA TTTCCCGAAC CACTGACGGG GTCTATGAAG GGGTGGCCAT TGGAGGAGAC 
AGATACCCTG GTTCAACTTT TATGGATCAT GTCTTGCGTT ATGAGGATAC TCCAGGAGTG 
AAAATGATTG TAGTACTGGG AGAGATTGGA GGCACAGAAG AGTATAAGAT CTGCAGGGGC 
ATTAAAGAAG GTCGTATCAC CAAGCCGGTG GTGTGTTGGT GTATTGGTAC TTGTGCCACC 
ATGTTCTCTT CAGAGGTGCA GTTTGGCCAC GCAGGAGCTT GTGCCAACCA AGCTTCTGAA 
ACTGCTGTTG CAAAGAATCA AGCCTTGAAG GAAGCTGGTG TGTTTGTTCC CCGGAGTTTT 
GACGAGCTGG GAGAGGTCAT TCAGTCTGTC TACCAGGATC TTGTGGCCAA AAGAGTGATT 
GAACCAGCTG AAGAAGTGCC TCCTCCAACT GTGCCAATGG ATTACTCCTG GGCAAGGGAG 
CTGGGTCTGA TCCGCAAACC GGCTTCCTTT ATGACAAGCA TTTGTGATGA GAGAGGTCAG 
GAACTGATTT ATGCTGGGAT GCCAATCACT GAGGTCTTCA AAGAAGAGAT GGGGATTGGA 
GGGGTTCTGG GCCTGCTCTG GTTTCAAAGG AGGTTACCAA AGTATGCCTG CCAGTTCATT 
GAGATGTGTT TGATGGTAAC AGCAGATCAT GGGCCTGCTG TTTCTGGAGC CCACAACACC 
ATTGTCTGTG CAAGAGCTGG AAAAGATCTT GTCTCGAGTC TCACTTCAGG CCTTCTTACT 
ATTGGTGACC GGTTTGGTGG AGCACTGGAT GCTGCAGCTA AAATGTTCAG CAAAGCCTTT 
GACAGTGGAA TTATCCCTAT GGAATTTGTG AACAAGATGA AGAAAGAAGG GAAACTGATC 
ATGGGCATTG GACACAGAGT CAAATCGATA AATAACCCAG ACATGAGAGT TCAGATTCTC 
AAAGACTACG TGAAGCAGCA TTTCCCTGCC ACCCCACTGT TGGACTATGC ACTTGAAGTA 
GAAAAAATTA CGACTTCCAA GAAACCAAAT CTTATCCTGA ATGTAGATGG CTTTATTGGA 
GTTGCCTTTG TTGACGTACT CAGGAATTGT GGCTCGTTCA CACGGGAAGA AGCAGATGAG 
TATATTGATA TAGGAGCTCT TAATGGCATC TTTGTACTAG GCAGGAGTAT GGGATTCATT 
GGACACTACC TGGATCAGAA GAGATTGAAG CAAGGCCTGT ACCGTCACCC GTGGGATGAC 
ATATCCTATG TTTTACCAGA GCACATGACT ATGTGA