BLMH cDNA ORF clone, Egretta garzetta(little egret)

Gene
Clones
gRNAs

Summary

Gene Symbol	BLMH
Entrez Gene ID	104121867
Full Name	bleomycin hydrolase
Gene Type	protein-coding
Organism	Egretta garzetta(little egret)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009633915.1	XP_009632210.1	bleomycin hydrolase

Pathways from BioSystems
Gene Ontology

The following BLMH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLMH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEg03217	XM_009633915.1 Latest version!	Egretta garzetta bleomycin hydrolase (BLMH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BLMH ( XM_009633915.1 ) cDNA ORF clone, Egretta garzetta(little egret) -> XP_009632210.1 Egretta garzetta bleomycin hydrolase (BLMH), mRNA.

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CloneID	OEg03217
Clone ID Related Accession (Same CDS sequence)	XM_009633915.1
Accession Version	XM_009633915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-15
Organism	Egretta garzetta(little egret)
Product	bleomycin hydrolase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009258884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Egretta garzetta Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

ATGCACAAAC TCTTCTCGGA GGAGCAGGGC TGTCACTCAA CAGGAGGCCT CCGCAGCGTG 
ACCTGGAGCA CGTCCTTGGC CGACGCTCAC CAGCGTGCAG GAAGGGCTGT GGCCACCCCG 
GGGAGATGTT GGATCTTTTC CTGTCTCAAT GCAATGCGTC TTCCTTTCAT GAAGAAATAC 
AACATTGAAG AGTTTGAATT CAGCCAGTCC TATCTCTTTT TCTGGGATAA GGTTGAACGC 
TGTTACTATT TTTTAAATGC CTTTGTGGAA ACAGCTCAGA AAAAGGAGCC TGTGGAGGGG 
AGACTGATAC AGTTCCTGCT TTCCAACCCC ACGAATGATG GTGGACAGTG GGATATGCTG 
GTTAACGTTA TTGAAAAGTA TGGTGTTGTG CCCAAGAAAT ACTTCCCGGA GTCTCACACC 
ACAGAGGCTA CTAGAAGGAT GAATGAGATC TTGAATCATA AGATGAGAGA ATACTGTTTG 
CGGCTAAGAA ATATGGTGGA AAGTGGAACG ATTAAAGGAG AACTTTGTGC TGCAATGGAC 
ACAATGATAG AAGAGGTATT CAGAATAGTG AGTACTTGCC TGGGCAGCCC TCCGGAGACC 
TTCTGCTGGG AGTTCCGGGA TAAGGAGAAG AACTACCACA AATATGGCCC TATGACACCG 
GTCCAGTTCT ACAATGAGCA CGTGAAGCCT TACTTCAACA TGGAGGACAA GATTTGTCTA 
GTGAATGATC CTCGACCTCA GAACCTGTAC AACAGGCTCT ATACAGTGGA GTACCTGGGC 
AACATGGTAG GAGGGAGGAA AACACTCTAT AACAACCAGC CTGTTGAAGT CTTGAAAAAA 
TTAGCTGCAG CATCTATTAA GGATGGCGAG GCTGTGTGGT TTGGCTGTGA TGTCGCAAAG 
CACTTCTATG GCAAGCTGGG AATCAATGAC ATGAATATAT TTAATCATGA GCTGGTGTTT 
GGTGTCTCTG TCAAGAACAT GAACAAAGCA GAACGATTAA TCTTTGGAGA ATCAATGATG 
ACCCATGCCA TGGTCCTGAC GGCTGTCACC GAGAAGGATG GGCAAGAGGA GGCATTTGAA 
AAATGGAGAG TGGAAAACTC CTGGGGTGAA GACCGTGGTA ATAAAGGTTA CCTGATCATG 
ACTGATGACT GGTTTTCTGA GTACGTGTAC GAAGTGGTGG TGGACAAGAA GCATGTACCG 
GAAGACATCT TGGCAGTGAT GCAGCAGGAA CCAATTGTTT TGCCAGCTTG GGACCCTATG 
GGGGCTTTAG CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009632210.1
CDS	1..1278
Translation	MHKLFSEEQGCHSTGGLRSVTWSTSLADAHQRAGRAVATPGRCWIFSCLNAMRLPFMKKYNIEEFEFSQSYLFFWDKVERCYYFLNAFVETAQKKEPVEGRLIQFLLSNPTNDGGQWDMLVNVIEKYGVVPKKYFPESHTTEATRRMNEILNHKMREYCLRLRNMVESGTIKGELCAAMDTMIEEVFRIVSTCLGSPPETFCWEFRDKEKNYHKYGPMTPVQFYNEHVKPYFNMEDKICLVNDPRPQNLYNRLYTVEYLGNMVGGRKTLYNNQPVEVLKKLAAASIKDGEAVWFGCDVAKHFYGKLGINDMNIFNHELVFGVSVKNMNKAERLIFGESMMTHAMVLTAVTEKDGQEEAFEKWRVENSWGEDRGNKGYLIMTDDWFSEYVYEVVVDKKHVPEDILAVMQQEPIVLPAWDPMGALAK

Target ORF information:

RefSeq Version	XM_009633915.1
Organism	Egretta garzetta(little egret)
Definition	Egretta garzetta bleomycin hydrolase (BLMH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009633915.1

ORF Insert Sequence:

ATGCACAAAC TCTTCTCGGA GGAGCAGGGC TGTCACTCAA CAGGAGGCCT CCGCAGCGTG 
ACCTGGAGCA CGTCCTTGGC CGACGCTCAC CAGCGTGCAG GAAGGGCTGT GGCCACCCCG 
GGGAGATGTT GGATCTTTTC CTGTCTCAAT GCAATGCGTC TTCCTTTCAT GAAGAAATAC 
AACATTGAAG AGTTTGAATT CAGCCAGTCC TATCTCTTTT TCTGGGATAA GGTTGAACGC 
TGTTACTATT TTTTAAATGC CTTTGTGGAA ACAGCTCAGA AAAAGGAGCC TGTGGAGGGG 
AGACTGATAC AGTTCCTGCT TTCCAACCCC ACGAATGATG GTGGACAGTG GGATATGCTG 
GTTAACGTTA TTGAAAAGTA TGGTGTTGTG CCCAAGAAAT ACTTCCCGGA GTCTCACACC 
ACAGAGGCTA CTAGAAGGAT GAATGAGATC TTGAATCATA AGATGAGAGA ATACTGTTTG 
CGGCTAAGAA ATATGGTGGA AAGTGGAACG ATTAAAGGAG AACTTTGTGC TGCAATGGAC 
ACAATGATAG AAGAGGTATT CAGAATAGTG AGTACTTGCC TGGGCAGCCC TCCGGAGACC 
TTCTGCTGGG AGTTCCGGGA TAAGGAGAAG AACTACCACA AATATGGCCC TATGACACCG 
GTCCAGTTCT ACAATGAGCA CGTGAAGCCT TACTTCAACA TGGAGGACAA GATTTGTCTA 
GTGAATGATC CTCGACCTCA GAACCTGTAC AACAGGCTCT ATACAGTGGA GTACCTGGGC 
AACATGGTAG GAGGGAGGAA AACACTCTAT AACAACCAGC CTGTTGAAGT CTTGAAAAAA 
TTAGCTGCAG CATCTATTAA GGATGGCGAG GCTGTGTGGT TTGGCTGTGA TGTCGCAAAG 
CACTTCTATG GCAAGCTGGG AATCAATGAC ATGAATATAT TTAATCATGA GCTGGTGTTT 
GGTGTCTCTG TCAAGAACAT GAACAAAGCA GAACGATTAA TCTTTGGAGA ATCAATGATG 
ACCCATGCCA TGGTCCTGAC GGCTGTCACC GAGAAGGATG GGCAAGAGGA GGCATTTGAA 
AAATGGAGAG TGGAAAACTC CTGGGGTGAA GACCGTGGTA ATAAAGGTTA CCTGATCATG 
ACTGATGACT GGTTTTCTGA GTACGTGTAC GAAGTGGTGG TGGACAAGAA GCATGTACCG 
GAAGACATCT TGGCAGTGAT GCAGCAGGAA CCAATTGTTT TGCCAGCTTG GGACCCTATG 
GGGGCTTTAG CCAAGTGA