LOC108152809 cDNA ORF clone, Drosophila miranda

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108152809
Entrez Gene ID	108152809
Full Name	prolyl 4-hydroxylase subunit alpha-1
Gene Type	protein-coding
Organism	Drosophila miranda

mRNA and Protein(s)

mRNA	Protein	Name
XM_017282396.1	XP_017137885.1	prolyl 4-hydroxylase subunit alpha-1

Pathways from BioSystems
Gene Ontology

The following LOC108152809 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108152809 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODr80880	XM_017282396.1 Latest version!	Drosophila miranda prolyl 4-hydroxylase subunit alpha-1 (LOC108152809), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108152809 ( XM_017282396.1 ) cDNA ORF clone, Drosophila miranda -> XP_017137885.1 Drosophila miranda prolyl 4-hydroxylase subunit alpha-1 (LOC108152809), mRNA.

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CloneID	ODr80880
Clone ID Related Accession (Same CDS sequence)	XM_017282396.1
Accession Version	XM_017282396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-15
Organism	Drosophila miranda
Product	prolyl 4-hydroxylase subunit alpha-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030303.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Drosophila miranda Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAC TTTTCGCCTT AATTTGCCTT ATAGTTTTAG TCCAAGACAG TCGCTCTGTT 
GATCCGGCGA ACTATTTTGT AGATTACTAC AGTTCTTCGG TTTTGGGATT GCTGAATCTT 
TTTGAGCTTG AGCGAGAATT TATGGACAAT TTCATAGTCT ATGCAGATGT CCTGCAAGAG 
AAGGTTGACA ACCTGAAAAT GTACATCGAT TCTTTGGATC GTGGTATGCA TGAAAACCAC 
AAGGAACGGG AAGGCTTTGT CTCCAACCCA CTCAACGCCT TTGGACTGAT CCGTCGCTTG 
CATCAGGAAT GGCCCAAGTG GCAGAACTAC ACCAAGAAGC CCCTGGGTGT GGCACAGCTA 
ACTGCAATGC AAGAGCTACT GTCTGAAGCG CCTGAACCTT ACGACATGAC AGAGTCCCTG 
AAGTCAATGG ATCGAATTGA GAGAGCTTAT GACCTCCCAG CCAGCCACAT AGCCAGGGGC 
CTGCTGCAGA ACAAACAATA CGACACTCAA CTATCAGTCC GGGACTGCTT GGCCTTGGCA 
CAGCACAAAT ACGAATCCGG CGACTATATA CGTGCCTCGA TGTGGTACCG CGAAGCCCTG 
CGCCACAATG GCGAACCCAA CGCCGATATC ATTAACAGCG TTCTGGGCAA TCCACTCGAA 
GGTGTGGATC TTCAGTTTGC CAGAACCATT GTCATACGCG GCCTTGGTTT GTCGGATCAT 
TCGATGAGCA GGGAAACGAT TCACGAATTA GCCAGCAACT TTTTGAACCA GTCCAGTCCA 
CGAGATGTGA AGATATTCAT CAACCATTAT CTCAGACAAA GTGATGAGCA AATAGGCAAT 
GAATTGGGTA CCGTCCAGAA GCCGCCAACT GCCCACGAAT CTGGATGTCG CGGACTGTTT 
CCCAAACGCA CGAATCTCGT CTGTCGCTAC AACAGCACCA CGACACCTTT CCTAAGACTG 
GCCCCCCTGA AAATGGAGGA GGTTAATCAC GATCCTTATA TCGTTATGTA CCACCAAGTT 
CTATCGGACC GAGAAATGGA GGAAATGAAG CAACTGGCAC AGCCAATGAC CAATGGAATG 
TCGGGGTCGG AATTGGCGAA TTTAACAGAG CCCCTCGAAA TTGTGGCACG CGTCGCATGG 
CTGATTGAAG CGTCGCCCTT CAGGGAGCGG CTCAATCTCC GCATCGGTGA CATGACGGGC 
TTCGATGTGA GTGACTTTAA AGCCCTCCAA TTGGCCAACT TTGGGGTGGG CTCCTACTTC 
AAGGCGCACT ACGACTACCG CACCGAACGC GTCAACGACT TGGGCGTCAC AGAGTTGGGC 
GATCGCACAG GCAGCATTAT CTTTTATGCC AGCGAAGTGC CCCAAGGTGG CGCCACGATA 
TTCCCCGACA TCCAGGTGAC GGTCACACCT CAGAAAGGAA ACAGCTTGTT CTGGTTCAAC 
ACATTCGATG ACTCGACCCC AGACCCGCGA TCCCTGCACG CCATATGCCC TGTCATAGCC 
GGTTCCAGAT GGACAATTAC CAAATGGCTC CACCAATGGC CACAAATGTT TCTGAAGCCC 
TGTAGTCCGC GGGCAGTGGA ACGGACGTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017137885.1
CDS	7..1599
Translation	MSRLFALICLIVLVQDSRSVDPANYFVDYYSSSVLGLLNLFELEREFMDNFIVYADVLQEKVDNLKMYIDSLDRGMHENHKEREGFVSNPLNAFGLIRRLHQEWPKWQNYTKKPLGVAQLTAMQELLSEAPEPYDMTESLKSMDRIERAYDLPASHIARGLLQNKQYDTQLSVRDCLALAQHKYESGDYIRASMWYREALRHNGEPNADIINSVLGNPLEGVDLQFARTIVIRGLGLSDHSMSRETIHELASNFLNQSSPRDVKIFINHYLRQSDEQIGNELGTVQKPPTAHESGCRGLFPKRTNLVCRYNSTTTPFLRLAPLKMEEVNHDPYIVMYHQVLSDREMEEMKQLAQPMTNGMSGSELANLTEPLEIVARVAWLIEASPFRERLNLRIGDMTGFDVSDFKALQLANFGVGSYFKAHYDYRTERVNDLGVTELGDRTGSIIFYASEVPQGGATIFPDIQVTVTPQKGNSLFWFNTFDDSTPDPRSLHAICPVIAGSRWTITKWLHQWPQMFLKPCSPRAVERTS

Target ORF information:

RefSeq Version	XM_017282396.1
Organism	Drosophila miranda
Definition	Drosophila miranda prolyl 4-hydroxylase subunit alpha-1 (LOC108152809), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017282396.1

ORF Insert Sequence:

ATGTCCAGAC TTTTCGCCTT AATTTGCCTT ATAGTTTTAG TCCAAGACAG TCGCTCTGTT 
GATCCGGCGA ACTATTTTGT AGATTACTAC AGTTCTTCGG TTTTGGGATT GCTGAATCTT 
TTTGAGCTTG AGCGAGAATT TATGGACAAT TTCATAGTCT ATGCAGATGT CCTGCAAGAG 
AAGGTTGACA ACCTGAAAAT GTACATCGAT TCTTTGGATC GTGGTATGCA TGAAAACCAC 
AAGGAACGGG AAGGCTTTGT CTCCAACCCA CTCAACGCCT TTGGACTGAT CCGTCGCTTG 
CATCAGGAAT GGCCCAAGTG GCAGAACTAC ACCAAGAAGC CCCTGGGTGT GGCACAGCTA 
ACTGCAATGC AAGAGCTACT GTCTGAAGCG CCTGAACCTT ACGACATGAC AGAGTCCCTG 
AAGTCAATGG ATCGAATTGA GAGAGCTTAT GACCTCCCAG CCAGCCACAT AGCCAGGGGC 
CTGCTGCAGA ACAAACAATA CGACACTCAA CTATCAGTCC GGGACTGCTT GGCCTTGGCA 
CAGCACAAAT ACGAATCCGG CGACTATATA CGTGCCTCGA TGTGGTACCG CGAAGCCCTG 
CGCCACAATG GCGAACCCAA CGCCGATATC ATTAACAGCG TTCTGGGCAA TCCACTCGAA 
GGTGTGGATC TTCAGTTTGC CAGAACCATT GTCATACGCG GCCTTGGTTT GTCGGATCAT 
TCGATGAGCA GGGAAACGAT TCACGAATTA GCCAGCAACT TTTTGAACCA GTCCAGTCCA 
CGAGATGTGA AGATATTCAT CAACCATTAT CTCAGACAAA GTGATGAGCA AATAGGCAAT 
GAATTGGGTA CCGTCCAGAA GCCGCCAACT GCCCACGAAT CTGGATGTCG CGGACTGTTT 
CCCAAACGCA CGAATCTCGT CTGTCGCTAC AACAGCACCA CGACACCTTT CCTAAGACTG 
GCCCCCCTGA AAATGGAGGA GGTTAATCAC GATCCTTATA TCGTTATGTA CCACCAAGTT 
CTATCGGACC GAGAAATGGA GGAAATGAAG CAACTGGCAC AGCCAATGAC CAATGGAATG 
TCGGGGTCGG AATTGGCGAA TTTAACAGAG CCCCTCGAAA TTGTGGCACG CGTCGCATGG 
CTGATTGAAG CGTCGCCCTT CAGGGAGCGG CTCAATCTCC GCATCGGTGA CATGACGGGC 
TTCGATGTGA GTGACTTTAA AGCCCTCCAA TTGGCCAACT TTGGGGTGGG CTCCTACTTC 
AAGGCGCACT ACGACTACCG CACCGAACGC GTCAACGACT TGGGCGTCAC AGAGTTGGGC 
GATCGCACAG GCAGCATTAT CTTTTATGCC AGCGAAGTGC CCCAAGGTGG CGCCACGATA 
TTCCCCGACA TCCAGGTGAC GGTCACACCT CAGAAAGGAA ACAGCTTGTT CTGGTTCAAC 
ACATTCGATG ACTCGACCCC AGACCCGCGA TCCCTGCACG CCATATGCCC TGTCATAGCC 
GGTTCCAGAT GGACAATTAC CAAATGGCTC CACCAATGGC CACAAATGTT TCTGAAGCCC 
TGTAGTCCGC GGGCAGTGGA ACGGACGTCT TAA