pont cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pont
Entrez Gene ID	53439
Full Name	pontin
Synonyms	0229/05,CG4003,Dmel\CG4003,PON,PONT,Pont,Pontin,RUVBL1,Tip49,Tip49/Pontin,anon-WO0118547.698,dPon,dPontin,dRUVBL1,dpon,l(3)S022905,pon
General protein information	Preferred Names pontin Names CG4003 gene product from transcript CG4003-RA CG4003-PA dpontin pont-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86A3-86A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_144351.4	NP_652608.1	pontin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RUVBL1	NP_001233458.1
	RUVBL1	XP_002798758.1
Gallus gallus (chicken)	RUVBL1	NP_001006138.1
Drosophila melanogaster (fruit fly)	pont	NP_652608.1
Caenorhabditis elegans (roundworm)	ruvb-1	NP_505567.2
Saccharomyces cerevisiae (baker's yeast)	RVB1	NP_010476.1
Eremothecium gossypii	AGOS_AGL119C	NP_986548.1
Homo sapiens (human)	RUVBL1	NP_003698.1
Mus musculus (house mouse)	Ruvbl1	NP_062659.1
Rattus norvegicus (Norway rat)	Ruvbl1	NP_671706.1
Magnaporthe oryzae (rice blast fungus)	MGG_03958	XP_003719906.1
Oryza sativa (rice)	Os01g0837500	NP_001044738.1
Xenopus tropicalis (tropical clawed frog)	ruvbl1	XP_002933113.1
Canis lupus familiaris (dog)	RUVBL1	XP_848712.1
Bos taurus (cattle)	RUVBL1	NP_001094546.1
Danio rerio (zebrafish)	ruvbl1	NP_776196.2
Kluyveromyces lactis	KLLA0D18502g	XP_453880.1
Schizosaccharomyces pombe (fission yeast)	rvb1	NP_594783.1
Neurospora crassa	NCU03482	XP_955769.1
Arabidopsis thaliana (thale cress)	RIN1	NP_197625.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WAC promotes the interaction between TTT and Pontin/Reptin in an energy-dependent manner, thereby promoting mTORC1 activity by facilitating mTORC1 dimerization and mTORC1-Rag interaction
Title: WAC Regulates mTOR Activity by Acting as an Adaptor for the TTT and Pontin/Reptin Complexes.

two newly identified factors that restrict diverse viruses, dXPO1 and dRUVBL1 contributed to antiviral defense at the organismal level in adult flies
Title: Genome-wide RNAi screen identifies broadly-acting host factors that inhibit arbovirus infection.

Pontin has a mitosis-specific function in regulating microtubule assembly
Title: Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin.

The enzymatic functions of Rept and Pont are indispensable for maintaining Hox gene expression states, highlighting the importance of these two antagonistic factors in transcriptional output.
Title: Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control.

reptin and pontin have conserved their ability to bind to Myc during evolution
Title: Myc interacts genetically with Tip48/Reptin and Tip49/Pontin to control growth and proliferation during Drosophila development.

The following pont gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pont cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08745	NM_144351.4 Latest version!	Drosophila melanogaster pontin (pont), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pont ( NM_144351.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652608.1 Drosophila melanogaster pontin (pont), mRNA.

Price & Availability↑

CloneID	OFa08745
Clone ID Related Accession (Same CDS sequence)	NM_144351.4
Accession Version	NM_144351.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pontin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_144351.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGATCG AGGAAGTCAA GAGCACCGTG CGCACCCAAC GCATCGCAGC CCACAGCCAT 
GTGAAGGGAT TGGGCCTGGA CGAGGTTGGA GCTGCCGTGC ACAGTGCAGC CGGACTGGTG 
GGTCAGAAGG CGGCACGCGA GGCAGCCGGC ATTGTGGTGG ACCTCATCAA ATCGAAAAAG 
ATGGCCGGAC GAGCGCTTCT GCTGGCTGGG CCACCAGGCA CGGGCAAAAC CGCCATTGCT 
CTGGCCATTG CCCAGGAGCT GGGTAACAAA GTGCCCTTCT GTCCCATGGT CGGATCGGAG 
GTATTCAGCA ATGAGATCAA GAAGACCGAG GTGCTAATGG AGAATTTCCG GCGCTCCATT 
GGTCTGCGCA TTCGTGAGAC CAAAGAGGTG TACGAGGGCG AGGTTACGGA ACTCACGCCC 
GTGGAAACCG AGAATCCTAT GGGTGGCTAT GGCAAGACTA TCAGCAATGT CGTCATCGGC 
TTGAAAACAG CCAAGGGAAC CAAGCAGCTC AAGCTGGATC CCAGCATCTT TGATGCCTTG 
CAAAAGGAAA AGGTGGAGGT GGGCGATGTC ATCTACATAG AGGCCAACAG TGGTGCCGTA 
AAGCGTCAGG GTCGCAGTGA CACCTTTGCC ACTGAGTTTG ATCTCGAAAC CGAGGAGTAT 
GTGCCGCTGC CCAAGGGCGA TGTCCACAAG AAGAAGGAGG TTATCCAGGA TGTTACTCTG 
CACGATCTGG ATGTGGCCAA TGCTCGTCCA CAGGGCGGCC AGGATGTGCT CTCCATGATG 
GGCCAGCTTA TGAAGCCGAA GAAAACTGAA ATCACTGACA AACTACGCAT GGAGATCAAC 
AAGGTGGTAA ACAAGTACAT TGACCAGGGC ATAGCCGAGC TGGTTCCCGG AGTTCTCTTC 
ATCGATGAAA TTCACATGCT TGATTTGGAG ACCTTCACCT ACCTACACAA GTCCCTGGAA 
TCTCCCATTG CACCAATTGT GATTTTTGCC ACCAATCGTG GACGCTGTGT TATTCGTGGC 
ACCACCGACA TAGTTTCGCC GCACGGAATT CCGCTAGATC TGCTCGATCG TTTGCTGATC 
ATCCGCACAC TACTCTACTC CACTGCCGAC ATGGAGCAGA TAATCAAGTT GCGCGCCCAG 
ACCGAGGGAC TGCAGCTGGA GGAGAACGCA TTTACACGTC TCAGCGAGAT TGGAACCAGC 
TCCACGCTGC GCTACGCCGT CCAGCTGCTG ACCCCGGCTC ATCAGATGTG CAAGGTGAAC 
GGACGCAACC AGATCAGCAA GGATGATATC GAGGATGTGC ACTCGCTCTT CCTTGACGCC 
AAGCGCTCCT CGAAGCATTT GTCCGAAAAG AACAATAAGT TTATGTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652608.1
CDS	221..1591
Translation	MKIEEVKSTVRTQRIAAHSHVKGLGLDEVGAAVHSAAGLVGQKAAREAAGIVVDLIKSKKMAGRALLLAGPPGTGKTAIALAIAQELGNKVPFCPMVGSEVFSNEIKKTEVLMENFRRSIGLRIRETKEVYEGEVTELTPVETENPMGGYGKTISNVVIGLKTAKGTKQLKLDPSIFDALQKEKVEVGDVIYIEANSGAVKRQGRSDTFATEFDLETEEYVPLPKGDVHKKKEVIQDVTLHDLDVANARPQGGQDVLSMMGQLMKPKKTEITDKLRMEINKVVNKYIDQGIAELVPGVLFIDEIHMLDLETFTYLHKSLESPIAPIVIFATNRGRCVIRGTTDIVSPHGIPLDLLDRLLIIRTLLYSTADMEQIIKLRAQTEGLQLEENAFTRLSEIGTSSTLRYAVQLLTPAHQMCKVNGRNQISKDDIEDVHSLFLDAKRSSKHLSEKNNKFML

Target ORF information:

RefSeq Version	NM_144351.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pontin (pont), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144351.4

ORF Insert Sequence:

ATGAAGATCG AGGAAGTCAA GAGCACCGTG CGCACCCAAC GCATCGCAGC CCACAGCCAT 
GTGAAGGGAT TGGGCCTGGA CGAGGTTGGA GCTGCCGTGC ACAGTGCAGC CGGACTGGTG 
GGTCAGAAGG CGGCACGCGA GGCAGCCGGC ATTGTGGTGG ACCTCATCAA ATCGAAAAAG 
ATGGCCGGAC GAGCGCTTCT GCTGGCTGGG CCACCAGGCA CGGGCAAAAC CGCCATTGCT 
CTGGCCATTG CCCAGGAGCT GGGTAACAAA GTGCCCTTCT GTCCCATGGT CGGATCGGAG 
GTATTCAGCA ATGAGATCAA GAAGACCGAG GTGCTAATGG AGAATTTCCG GCGCTCCATT 
GGTCTGCGCA TTCGTGAGAC CAAAGAGGTG TACGAGGGCG AGGTTACGGA ACTCACGCCC 
GTGGAAACCG AGAATCCTAT GGGTGGCTAT GGCAAGACTA TCAGCAATGT CGTCATCGGC 
TTGAAAACAG CCAAGGGAAC CAAGCAGCTC AAGCTGGATC CCAGCATCTT TGATGCCTTG 
CAAAAGGAAA AGGTGGAGGT GGGCGATGTC ATCTACATAG AGGCCAACAG TGGTGCCGTA 
AAGCGTCAGG GTCGCAGTGA CACCTTTGCC ACTGAGTTTG ATCTCGAAAC CGAGGAGTAT 
GTGCCGCTGC CCAAGGGCGA TGTCCACAAG AAGAAGGAGG TTATCCAGGA TGTTACTCTG 
CACGATCTGG ATGTGGCCAA TGCTCGTCCA CAGGGCGGCC AGGATGTGCT CTCCATGATG 
GGCCAGCTTA TGAAGCCGAA GAAAACTGAA ATCACTGACA AACTACGCAT GGAGATCAAC 
AAGGTGGTAA ACAAGTACAT TGACCAGGGC ATAGCCGAGC TGGTTCCCGG AGTTCTCTTC 
ATCGATGAAA TTCACATGCT TGATTTGGAG ACCTTCACCT ACCTACACAA GTCCCTGGAA 
TCTCCCATTG CACCAATTGT GATTTTTGCC ACCAATCGTG GACGCTGTGT TATTCGTGGC 
ACCACCGACA TAGTTTCGCC GCACGGAATT CCGCTAGATC TGCTCGATCG TTTGCTGATC 
ATCCGCACAC TACTCTACTC CACTGCCGAC ATGGAGCAGA TAATCAAGTT GCGCGCCCAG 
ACCGAGGGAC TGCAGCTGGA GGAGAACGCA TTTACACGTC TCAGCGAGAT TGGAACCAGC 
TCCACGCTGC GCTACGCCGT CCAGCTGCTG ACCCCGGCTC ATCAGATGTG CAAGGTGAAC 
GGACGCAACC AGATCAGCAA GGATGATATC GAGGATGTGC ACTCGCTCTT CCTTGACGCC 
AAGCGCTCCT CGAAGCATTT GTCCGAAAAG AACAATAAGT TTATGTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pont cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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