unc-45 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	unc-45
Entrez Gene ID	44910
Full Name	uncoordinated 45
Synonyms	CG2708,DmUNC-45,DmUnc-45,Dmel\CG2708,Tom34,dUNC-45,dunc-45,l(3)03692,lincRNA.721
General protein information	Preferred Names uncoordinated 45 Names CG2708 gene product from transcript CG2708-RB CG2708-PA CG2708-PB translocase of outer membrane 34 unc-45-PA unc-45-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84E9-84E9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316496.1	NP_001303425.1	uncoordinated 45, isoform B
NM_080057.4	NP_524796.1	uncoordinated 45, isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Unc45b	NP_001100498.1
Danio rerio (zebrafish)	unc45b	NP_705959.1
Drosophila melanogaster (fruit fly)	unc-45	NP_524796.1
Pan troglodytes (chimpanzee)	UNC45B	XP_001174363.2
Mus musculus (house mouse)	Unc45b	NP_848795.3
Gallus gallus (chicken)	UNC45B	XP_004946569.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003727	XP_310258.5
Canis lupus familiaris (dog)	UNC45B	XP_005624856.1
Bos taurus (cattle)	UNC45B	XP_002695676.1
Xenopus tropicalis (tropical clawed frog)	unc45b	NP_001172057.1
Homo sapiens (human)	UNC45B	NP_775259.1
	UNC45B	XP_001113905.2
Caenorhabditis elegans (roundworm)	unc-45	NP_497205.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

UCS proteins, such as UNC-45, influence muscle contraction and other myosin-dependent motile processes. DmUNC-45 function is afforded by its flexibility and by structural integrity of its UCS domain.
Title: X-ray crystal structure of the UCS domain-containing UNC-45 myosin chaperone from Drosophila melanogaster.

Data indicate that dUNC-45 is important for myosin accumulation and muscle function, and suggest that dUNC-45 acts post-translationally for proper myosin folding and maturation.
Title: Drosophila UNC-45 accumulates in embryonic blastoderm and in muscles, and is essential for muscle myosin stability.

UNC-45 plays a crucial role in stabilizing myosin
Title: The UNC-45 chaperone is critical for establishing myosin-based myofibrillar organization and cardiac contractility in the Drosophila heart model.

DUNC-45 prevents inter- or intra-molecular aggregates of skeletal muscle heavy meromyosin caused by elevated temperatures.
Title: Drosophila UNC-45 prevents heat-induced aggregation of skeletal muscle myosin and facilitates refolding of citrate synthase.

The following unc-45 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc-45 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06868	NM_001316496.1 Latest version!	Drosophila melanogaster uncoordinated 45 (unc-45), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06868	NM_080057.4 Latest version!	Drosophila melanogaster uncoordinated 45 (unc-45), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

unc-45 ( NM_001316496.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303425.1 Drosophila melanogaster uncoordinated 45 (unc-45), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06868
Clone ID Related Accession (Same CDS sequence)	NM_001316496.1 , NM_080057.4
Accession Version	NM_001316496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncoordinated 45, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAACA CCATCAACAG CGAGGAAGTG TCCGACGCAG GAAGCTACAA AGATAAGGGC 
AACGAGGCGT TCAAGGCCTC CCGCTGGGAG GAGGCAGTGG AGCACTATGG CAAAGCCATT 
AAGGCGGGCT CTAAGCACAA GGAGCTGGCG GTTTTCTATA AGAATCGTGC TGCCGCTTAT 
CTGAAGCTAG GAAAGTATGA AAATGCGGTA GAGGACTGCA CTGAATCTTT GAAAGCGGCT 
CCGGGGGATC CCAAGGCACT GTTCCGTAGG GCTCAGGCGT ACGAAGCTCT GGAGAAGTTC 
GAAGAGGCCT ACAAAGACGC AACCGCTTTG TTTAAAGCGG ATCCCGGCAA CAAAACCGTA 
CAGCCCATGC TCCAGAGGCT TCATGTCGTC GTGGAAGAGC GCTCTGCCCG TAATGCCAAG 
ACATCCACAA AAGTCAAGCA GATGATGGAT CTTACTTTTG ATTTGGCCAC GCCCATCGAT 
AAGCGTCGCG CGGCAGCCAA TAACCTAGTA GTTTTGGCCA AAGAGCAGAC TGGCGCTGAA 
CTGCTTTACA AGGACCACTG CATCGCCAAA GTGGCCTCAC TAACAAAGGT GGAAAAGGAT 
CAGGACATTT ATGTGAATAT GGTGCATTTG GTGGCTGCTC TTTGCGAAAA TAGCGTCGAG 
CGTACAAAGG GCGTTTTAAC TGAACTCGGA GTACCGTGGT TCATGAGGGT TCTCGACCAA 
AAACACGAGA ACTGTGTGTC CACCGCCCAG TTTTGCCTAC AAACAATATT GAACGCATTG 
TCTGGGCTTA AGAACAAACC CGATTCGAAG CCGGAGAAGG AGCTGTGCAC TAGGAACAAC 
CGGGAAATTG ACACTCTCCT AACATGCCTA GTTTATAGTA TCACGGATCG CACGATTTCT 
GGAGCAGCTA GGGATGGCGT TATTGAGCTT ATAACCAGGA ATGTCCACTA CACAGCTCTA 
GAATGGGCCG AACGCCTGGT TGAGATCAGG GGACTGTGCC GTCTGCTGGA TGTGTGCTCC 
GAACTGGAGG ATTATAAGTA CGAGAGTGCA ATGGATATCA CAGGCTCATC ATCCACAATT 
GCATCCGTTT GTCTGGCCCG GATATATGAG AACATGTACT ACGATGAAGC TAAGGCACGG 
TTCACGGACC AGATCGACGA ATACATTAAG GACAAGCTTT TGGCGCCTGA TATGGAATCC 
AAGGTGCGGG TCACCGTTGC GATTACTGCC CTGCTTAACG GTCCATTGGA TGTGGGCAAT 
CAGGTTGTGG CCAGAGAAGG AATACTGCAG ATGATTCTAG CAATGGCTAC GACCGACGAT 
GAGTTGCAGC AGCGAGTATC ATGTGAGTGC CTAATCGCCG CTTCCTCTAA AAAGGACAAG 
GCCAAGGCTC TTTGTGAGCA GGGAGTAGAT ATTTTAAAGC GGCTCTACCA CTCCAAGAAC 
GATGGTATTA GAGTACGCGC CCTGGTGGGT CTTTGCAAGC TGGGTAGCTA CGGCGGTCAG 
GACGCGGCCA TCCGACCATT TGGCGATGGA GCTGCCCTTA AGCTGGCGGA GGCATGCCGT 
CGGTTTTTGA TCAAACCTGG AAAGGATAAG GATATCCGTC GTTGGGCTGC CGATGGTCTG 
GCTTATTTAA CACTGGATGC TGAATGTAAG GAGAAGCTCA TTGAGGATAA GGCTTCCATT 
CACGCTTTAA TGGATTTGGC CCGCGGAGGA AATCAGTCCT GTCTCTATGG TGTGGTCACT 
ACTTTTGTGA ATCTGTGCAA TGCGTATGAG AAGCAGGAGA TGTTGCCTGA GATGATAGAA 
CTTGCCAAGT TTGCTAAGCA ACACATACCG GAGGAGCACG AGCTGGACGA CGTAGATTTC 
ATCAATAAGC GTATTACTGT GCTGGCAAAT GAGGGCATAA CAACTGCTCT TTGTGCACTG 
GCGAAAACTG AAAGCCACAA TTCACAGGAG CTGATTGCCA GGGTTTTGAA TGCGGTTTGT 
GGACTGAAAG AGTTGCGAGG AAAAGTGGTT CAAGAAGGCG GCGTTAAGGC GCTGCTTCGT 
ATGGCGCTTG AGGGAACTGA GAAAGGAAAA CGCCATGCTA CCCAGGCGTT GGCTAGGATT 
GGCATAACCA TTAATCCGGA AGTATCTTTC AGTGGCCAGC GATCGCTTGA TGTAATCCGT 
CCTTTGTTGA ACCTTTTGCA ACAGGACTGC ACAGCGCTGG AGAATTTTGA GTCGCTAATG 
GCACTCACCA ATCTAGCCAG CATGAACGAG AGCGTGCGCC AGAGGATTAT CAAGGAGCAG 
GGGGTCTCCA AGATAGAGTA TTATCTGATG GAGGACCATC TGTACCTGAC TCGTGCAGCT 
GCCCAGTGTC TGTGTAATCT GGTTATGAGT GAGGATGTCA TTAAAATGTT CGAGGGCAAT 
AACGACCGAG TGAAGTTCTT GGCGCTTCTC TGCGAGGATG AGGACGAGGA GACCGCAACA 
GCTTGTGCCG GAGCTCTGGC CATTATTACT TCAGTGTCTG TCAAGTGCTG TGAGAAGATC 
TTGGCCATCG CCAGCTGGCT GGACATTCTG CACACTCTGA TCGCCAATCC CAGTCCGGCG 
GTCCAGCATC GAGGCATTGT GATTATTCTA AACATGATCA ATGCTGGAGA GGAGATCGCC 
AAAAAGCTGT TTGAAACGGA CATTATGGAA CTGCTGAGCG GTTTGGGGCA GCTGCCCGAT 
GACACGCGTG CCAAGGCCCG AGAGGTGGCC ACCCAGTGCC TGGCGGCGGC GGAGCGTTAT 
AGGATCATCG AGCGGTCCGA CAACGCGGAA ATCCCCGATG TGTTTGCCGA AAATTCGAAA 
ATATCTGAGA TTATCGATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303425.1
CDS	245..3088
Translation	MTNTINSEEVSDAGSYKDKGNEAFKASRWEEAVEHYGKAIKAGSKHKELAVFYKNRAAAYLKLGKYENAVEDCTESLKAAPGDPKALFRRAQAYEALEKFEEAYKDATALFKADPGNKTVQPMLQRLHVVVEERSARNAKTSTKVKQMMDLTFDLATPIDKRRAAANNLVVLAKEQTGAELLYKDHCIAKVASLTKVEKDQDIYVNMVHLVAALCENSVERTKGVLTELGVPWFMRVLDQKHENCVSTAQFCLQTILNALSGLKNKPDSKPEKELCTRNNREIDTLLTCLVYSITDRTISGAARDGVIELITRNVHYTALEWAERLVEIRGLCRLLDVCSELEDYKYESAMDITGSSSTIASVCLARIYENMYYDEAKARFTDQIDEYIKDKLLAPDMESKVRVTVAITALLNGPLDVGNQVVAREGILQMILAMATTDDELQQRVSCECLIAASSKKDKAKALCEQGVDILKRLYHSKNDGIRVRALVGLCKLGSYGGQDAAIRPFGDGAALKLAEACRRFLIKPGKDKDIRRWAADGLAYLTLDAECKEKLIEDKASIHALMDLARGGNQSCLYGVVTTFVNLCNAYEKQEMLPEMIELAKFAKQHIPEEHELDDVDFINKRITVLANEGITTALCALAKTESHNSQELIARVLNAVCGLKELRGKVVQEGGVKALLRMALEGTEKGKRHATQALARIGITINPEVSFSGQRSLDVIRPLLNLLQQDCTALENFESLMALTNLASMNESVRQRIIKEQGVSKIEYYLMEDHLYLTRAAAQCLCNLVMSEDVIKMFEGNNDRVKFLALLCEDEDEETATACAGALAIITSVSVKCCEKILAIASWLDILHTLIANPSPAVQHRGIVIILNMINAGEEIAKKLFETDIMELLSGLGQLPDDTRAKAREVATQCLAAAERYRIIERSDNAEIPDVFAENSKISEIIDD

Target ORF information:

RefSeq Version	NM_001316496.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncoordinated 45 (unc-45), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316496.1

ORF Insert Sequence:

ATGACAAACA CCATCAACAG CGAGGAAGTG TCCGACGCAG GAAGCTACAA AGATAAGGGC 
AACGAGGCGT TCAAGGCCTC CCGCTGGGAG GAGGCAGTGG AGCACTATGG CAAAGCCATT 
AAGGCGGGCT CTAAGCACAA GGAGCTGGCG GTTTTCTATA AGAATCGTGC TGCCGCTTAT 
CTGAAGCTAG GAAAGTATGA AAATGCGGTA GAGGACTGCA CTGAATCTTT GAAAGCGGCT 
CCGGGGGATC CCAAGGCACT GTTCCGTAGG GCTCAGGCGT ACGAAGCTCT GGAGAAGTTC 
GAAGAGGCCT ACAAAGACGC AACCGCTTTG TTTAAAGCGG ATCCCGGCAA CAAAACCGTA 
CAGCCCATGC TCCAGAGGCT TCATGTCGTC GTGGAAGAGC GCTCTGCCCG TAATGCCAAG 
ACATCCACAA AAGTCAAGCA GATGATGGAT CTTACTTTTG ATTTGGCCAC GCCCATCGAT 
AAGCGTCGCG CGGCAGCCAA TAACCTAGTA GTTTTGGCCA AAGAGCAGAC TGGCGCTGAA 
CTGCTTTACA AGGACCACTG CATCGCCAAA GTGGCCTCAC TAACAAAGGT GGAAAAGGAT 
CAGGACATTT ATGTGAATAT GGTGCATTTG GTGGCTGCTC TTTGCGAAAA TAGCGTCGAG 
CGTACAAAGG GCGTTTTAAC TGAACTCGGA GTACCGTGGT TCATGAGGGT TCTCGACCAA 
AAACACGAGA ACTGTGTGTC CACCGCCCAG TTTTGCCTAC AAACAATATT GAACGCATTG 
TCTGGGCTTA AGAACAAACC CGATTCGAAG CCGGAGAAGG AGCTGTGCAC TAGGAACAAC 
CGGGAAATTG ACACTCTCCT AACATGCCTA GTTTATAGTA TCACGGATCG CACGATTTCT 
GGAGCAGCTA GGGATGGCGT TATTGAGCTT ATAACCAGGA ATGTCCACTA CACAGCTCTA 
GAATGGGCCG AACGCCTGGT TGAGATCAGG GGACTGTGCC GTCTGCTGGA TGTGTGCTCC 
GAACTGGAGG ATTATAAGTA CGAGAGTGCA ATGGATATCA CAGGCTCATC ATCCACAATT 
GCATCCGTTT GTCTGGCCCG GATATATGAG AACATGTACT ACGATGAAGC TAAGGCACGG 
TTCACGGACC AGATCGACGA ATACATTAAG GACAAGCTTT TGGCGCCTGA TATGGAATCC 
AAGGTGCGGG TCACCGTTGC GATTACTGCC CTGCTTAACG GTCCATTGGA TGTGGGCAAT 
CAGGTTGTGG CCAGAGAAGG AATACTGCAG ATGATTCTAG CAATGGCTAC GACCGACGAT 
GAGTTGCAGC AGCGAGTATC ATGTGAGTGC CTAATCGCCG CTTCCTCTAA AAAGGACAAG 
GCCAAGGCTC TTTGTGAGCA GGGAGTAGAT ATTTTAAAGC GGCTCTACCA CTCCAAGAAC 
GATGGTATTA GAGTACGCGC CCTGGTGGGT CTTTGCAAGC TGGGTAGCTA CGGCGGTCAG 
GACGCGGCCA TCCGACCATT TGGCGATGGA GCTGCCCTTA AGCTGGCGGA GGCATGCCGT 
CGGTTTTTGA TCAAACCTGG AAAGGATAAG GATATCCGTC GTTGGGCTGC CGATGGTCTG 
GCTTATTTAA CACTGGATGC TGAATGTAAG GAGAAGCTCA TTGAGGATAA GGCTTCCATT 
CACGCTTTAA TGGATTTGGC CCGCGGAGGA AATCAGTCCT GTCTCTATGG TGTGGTCACT 
ACTTTTGTGA ATCTGTGCAA TGCGTATGAG AAGCAGGAGA TGTTGCCTGA GATGATAGAA 
CTTGCCAAGT TTGCTAAGCA ACACATACCG GAGGAGCACG AGCTGGACGA CGTAGATTTC 
ATCAATAAGC GTATTACTGT GCTGGCAAAT GAGGGCATAA CAACTGCTCT TTGTGCACTG 
GCGAAAACTG AAAGCCACAA TTCACAGGAG CTGATTGCCA GGGTTTTGAA TGCGGTTTGT 
GGACTGAAAG AGTTGCGAGG AAAAGTGGTT CAAGAAGGCG GCGTTAAGGC GCTGCTTCGT 
ATGGCGCTTG AGGGAACTGA GAAAGGAAAA CGCCATGCTA CCCAGGCGTT GGCTAGGATT 
GGCATAACCA TTAATCCGGA AGTATCTTTC AGTGGCCAGC GATCGCTTGA TGTAATCCGT 
CCTTTGTTGA ACCTTTTGCA ACAGGACTGC ACAGCGCTGG AGAATTTTGA GTCGCTAATG 
GCACTCACCA ATCTAGCCAG CATGAACGAG AGCGTGCGCC AGAGGATTAT CAAGGAGCAG 
GGGGTCTCCA AGATAGAGTA TTATCTGATG GAGGACCATC TGTACCTGAC TCGTGCAGCT 
GCCCAGTGTC TGTGTAATCT GGTTATGAGT GAGGATGTCA TTAAAATGTT CGAGGGCAAT 
AACGACCGAG TGAAGTTCTT GGCGCTTCTC TGCGAGGATG AGGACGAGGA GACCGCAACA 
GCTTGTGCCG GAGCTCTGGC CATTATTACT TCAGTGTCTG TCAAGTGCTG TGAGAAGATC 
TTGGCCATCG CCAGCTGGCT GGACATTCTG CACACTCTGA TCGCCAATCC CAGTCCGGCG 
GTCCAGCATC GAGGCATTGT GATTATTCTA AACATGATCA ATGCTGGAGA GGAGATCGCC 
AAAAAGCTGT TTGAAACGGA CATTATGGAA CTGCTGAGCG GTTTGGGGCA GCTGCCCGAT 
GACACGCGTG CCAAGGCCCG AGAGGTGGCC ACCCAGTGCC TGGCGGCGGC GGAGCGTTAT 
AGGATCATCG AGCGGTCCGA CAACGCGGAA ATCCCCGATG TGTTTGCCGA AAATTCGAAA 
ATATCTGAGA TTATCGATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-45 ( NM_080057.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524796.1 Drosophila melanogaster uncoordinated 45 (unc-45), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06868
Clone ID Related Accession (Same CDS sequence)	NM_001316496.1 , NM_080057.4
Accession Version	NM_080057.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncoordinated 45, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_080057.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAACA CCATCAACAG CGAGGAAGTG TCCGACGCAG GAAGCTACAA AGATAAGGGC 
AACGAGGCGT TCAAGGCCTC CCGCTGGGAG GAGGCAGTGG AGCACTATGG CAAAGCCATT 
AAGGCGGGCT CTAAGCACAA GGAGCTGGCG GTTTTCTATA AGAATCGTGC TGCCGCTTAT 
CTGAAGCTAG GAAAGTATGA AAATGCGGTA GAGGACTGCA CTGAATCTTT GAAAGCGGCT 
CCGGGGGATC CCAAGGCACT GTTCCGTAGG GCTCAGGCGT ACGAAGCTCT GGAGAAGTTC 
GAAGAGGCCT ACAAAGACGC AACCGCTTTG TTTAAAGCGG ATCCCGGCAA CAAAACCGTA 
CAGCCCATGC TCCAGAGGCT TCATGTCGTC GTGGAAGAGC GCTCTGCCCG TAATGCCAAG 
ACATCCACAA AAGTCAAGCA GATGATGGAT CTTACTTTTG ATTTGGCCAC GCCCATCGAT 
AAGCGTCGCG CGGCAGCCAA TAACCTAGTA GTTTTGGCCA AAGAGCAGAC TGGCGCTGAA 
CTGCTTTACA AGGACCACTG CATCGCCAAA GTGGCCTCAC TAACAAAGGT GGAAAAGGAT 
CAGGACATTT ATGTGAATAT GGTGCATTTG GTGGCTGCTC TTTGCGAAAA TAGCGTCGAG 
CGTACAAAGG GCGTTTTAAC TGAACTCGGA GTACCGTGGT TCATGAGGGT TCTCGACCAA 
AAACACGAGA ACTGTGTGTC CACCGCCCAG TTTTGCCTAC AAACAATATT GAACGCATTG 
TCTGGGCTTA AGAACAAACC CGATTCGAAG CCGGAGAAGG AGCTGTGCAC TAGGAACAAC 
CGGGAAATTG ACACTCTCCT AACATGCCTA GTTTATAGTA TCACGGATCG CACGATTTCT 
GGAGCAGCTA GGGATGGCGT TATTGAGCTT ATAACCAGGA ATGTCCACTA CACAGCTCTA 
GAATGGGCCG AACGCCTGGT TGAGATCAGG GGACTGTGCC GTCTGCTGGA TGTGTGCTCC 
GAACTGGAGG ATTATAAGTA CGAGAGTGCA ATGGATATCA CAGGCTCATC ATCCACAATT 
GCATCCGTTT GTCTGGCCCG GATATATGAG AACATGTACT ACGATGAAGC TAAGGCACGG 
TTCACGGACC AGATCGACGA ATACATTAAG GACAAGCTTT TGGCGCCTGA TATGGAATCC 
AAGGTGCGGG TCACCGTTGC GATTACTGCC CTGCTTAACG GTCCATTGGA TGTGGGCAAT 
CAGGTTGTGG CCAGAGAAGG AATACTGCAG ATGATTCTAG CAATGGCTAC GACCGACGAT 
GAGTTGCAGC AGCGAGTATC ATGTGAGTGC CTAATCGCCG CTTCCTCTAA AAAGGACAAG 
GCCAAGGCTC TTTGTGAGCA GGGAGTAGAT ATTTTAAAGC GGCTCTACCA CTCCAAGAAC 
GATGGTATTA GAGTACGCGC CCTGGTGGGT CTTTGCAAGC TGGGTAGCTA CGGCGGTCAG 
GACGCGGCCA TCCGACCATT TGGCGATGGA GCTGCCCTTA AGCTGGCGGA GGCATGCCGT 
CGGTTTTTGA TCAAACCTGG AAAGGATAAG GATATCCGTC GTTGGGCTGC CGATGGTCTG 
GCTTATTTAA CACTGGATGC TGAATGTAAG GAGAAGCTCA TTGAGGATAA GGCTTCCATT 
CACGCTTTAA TGGATTTGGC CCGCGGAGGA AATCAGTCCT GTCTCTATGG TGTGGTCACT 
ACTTTTGTGA ATCTGTGCAA TGCGTATGAG AAGCAGGAGA TGTTGCCTGA GATGATAGAA 
CTTGCCAAGT TTGCTAAGCA ACACATACCG GAGGAGCACG AGCTGGACGA CGTAGATTTC 
ATCAATAAGC GTATTACTGT GCTGGCAAAT GAGGGCATAA CAACTGCTCT TTGTGCACTG 
GCGAAAACTG AAAGCCACAA TTCACAGGAG CTGATTGCCA GGGTTTTGAA TGCGGTTTGT 
GGACTGAAAG AGTTGCGAGG AAAAGTGGTT CAAGAAGGCG GCGTTAAGGC GCTGCTTCGT 
ATGGCGCTTG AGGGAACTGA GAAAGGAAAA CGCCATGCTA CCCAGGCGTT GGCTAGGATT 
GGCATAACCA TTAATCCGGA AGTATCTTTC AGTGGCCAGC GATCGCTTGA TGTAATCCGT 
CCTTTGTTGA ACCTTTTGCA ACAGGACTGC ACAGCGCTGG AGAATTTTGA GTCGCTAATG 
GCACTCACCA ATCTAGCCAG CATGAACGAG AGCGTGCGCC AGAGGATTAT CAAGGAGCAG 
GGGGTCTCCA AGATAGAGTA TTATCTGATG GAGGACCATC TGTACCTGAC TCGTGCAGCT 
GCCCAGTGTC TGTGTAATCT GGTTATGAGT GAGGATGTCA TTAAAATGTT CGAGGGCAAT 
AACGACCGAG TGAAGTTCTT GGCGCTTCTC TGCGAGGATG AGGACGAGGA GACCGCAACA 
GCTTGTGCCG GAGCTCTGGC CATTATTACT TCAGTGTCTG TCAAGTGCTG TGAGAAGATC 
TTGGCCATCG CCAGCTGGCT GGACATTCTG CACACTCTGA TCGCCAATCC CAGTCCGGCG 
GTCCAGCATC GAGGCATTGT GATTATTCTA AACATGATCA ATGCTGGAGA GGAGATCGCC 
AAAAAGCTGT TTGAAACGGA CATTATGGAA CTGCTGAGCG GTTTGGGGCA GCTGCCCGAT 
GACACGCGTG CCAAGGCCCG AGAGGTGGCC ACCCAGTGCC TGGCGGCGGC GGAGCGTTAT 
AGGATCATCG AGCGGTCCGA CAACGCGGAA ATCCCCGATG TGTTTGCCGA AAATTCGAAA 
ATATCTGAGA TTATCGATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524796.1
CDS	245..3088
Translation	MTNTINSEEVSDAGSYKDKGNEAFKASRWEEAVEHYGKAIKAGSKHKELAVFYKNRAAAYLKLGKYENAVEDCTESLKAAPGDPKALFRRAQAYEALEKFEEAYKDATALFKADPGNKTVQPMLQRLHVVVEERSARNAKTSTKVKQMMDLTFDLATPIDKRRAAANNLVVLAKEQTGAELLYKDHCIAKVASLTKVEKDQDIYVNMVHLVAALCENSVERTKGVLTELGVPWFMRVLDQKHENCVSTAQFCLQTILNALSGLKNKPDSKPEKELCTRNNREIDTLLTCLVYSITDRTISGAARDGVIELITRNVHYTALEWAERLVEIRGLCRLLDVCSELEDYKYESAMDITGSSSTIASVCLARIYENMYYDEAKARFTDQIDEYIKDKLLAPDMESKVRVTVAITALLNGPLDVGNQVVAREGILQMILAMATTDDELQQRVSCECLIAASSKKDKAKALCEQGVDILKRLYHSKNDGIRVRALVGLCKLGSYGGQDAAIRPFGDGAALKLAEACRRFLIKPGKDKDIRRWAADGLAYLTLDAECKEKLIEDKASIHALMDLARGGNQSCLYGVVTTFVNLCNAYEKQEMLPEMIELAKFAKQHIPEEHELDDVDFINKRITVLANEGITTALCALAKTESHNSQELIARVLNAVCGLKELRGKVVQEGGVKALLRMALEGTEKGKRHATQALARIGITINPEVSFSGQRSLDVIRPLLNLLQQDCTALENFESLMALTNLASMNESVRQRIIKEQGVSKIEYYLMEDHLYLTRAAAQCLCNLVMSEDVIKMFEGNNDRVKFLALLCEDEDEETATACAGALAIITSVSVKCCEKILAIASWLDILHTLIANPSPAVQHRGIVIILNMINAGEEIAKKLFETDIMELLSGLGQLPDDTRAKAREVATQCLAAAERYRIIERSDNAEIPDVFAENSKISEIIDD

Target ORF information:

RefSeq Version	NM_080057.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncoordinated 45 (unc-45), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080057.4

ORF Insert Sequence:

ATGACAAACA CCATCAACAG CGAGGAAGTG TCCGACGCAG GAAGCTACAA AGATAAGGGC 
AACGAGGCGT TCAAGGCCTC CCGCTGGGAG GAGGCAGTGG AGCACTATGG CAAAGCCATT 
AAGGCGGGCT CTAAGCACAA GGAGCTGGCG GTTTTCTATA AGAATCGTGC TGCCGCTTAT 
CTGAAGCTAG GAAAGTATGA AAATGCGGTA GAGGACTGCA CTGAATCTTT GAAAGCGGCT 
CCGGGGGATC CCAAGGCACT GTTCCGTAGG GCTCAGGCGT ACGAAGCTCT GGAGAAGTTC 
GAAGAGGCCT ACAAAGACGC AACCGCTTTG TTTAAAGCGG ATCCCGGCAA CAAAACCGTA 
CAGCCCATGC TCCAGAGGCT TCATGTCGTC GTGGAAGAGC GCTCTGCCCG TAATGCCAAG 
ACATCCACAA AAGTCAAGCA GATGATGGAT CTTACTTTTG ATTTGGCCAC GCCCATCGAT 
AAGCGTCGCG CGGCAGCCAA TAACCTAGTA GTTTTGGCCA AAGAGCAGAC TGGCGCTGAA 
CTGCTTTACA AGGACCACTG CATCGCCAAA GTGGCCTCAC TAACAAAGGT GGAAAAGGAT 
CAGGACATTT ATGTGAATAT GGTGCATTTG GTGGCTGCTC TTTGCGAAAA TAGCGTCGAG 
CGTACAAAGG GCGTTTTAAC TGAACTCGGA GTACCGTGGT TCATGAGGGT TCTCGACCAA 
AAACACGAGA ACTGTGTGTC CACCGCCCAG TTTTGCCTAC AAACAATATT GAACGCATTG 
TCTGGGCTTA AGAACAAACC CGATTCGAAG CCGGAGAAGG AGCTGTGCAC TAGGAACAAC 
CGGGAAATTG ACACTCTCCT AACATGCCTA GTTTATAGTA TCACGGATCG CACGATTTCT 
GGAGCAGCTA GGGATGGCGT TATTGAGCTT ATAACCAGGA ATGTCCACTA CACAGCTCTA 
GAATGGGCCG AACGCCTGGT TGAGATCAGG GGACTGTGCC GTCTGCTGGA TGTGTGCTCC 
GAACTGGAGG ATTATAAGTA CGAGAGTGCA ATGGATATCA CAGGCTCATC ATCCACAATT 
GCATCCGTTT GTCTGGCCCG GATATATGAG AACATGTACT ACGATGAAGC TAAGGCACGG 
TTCACGGACC AGATCGACGA ATACATTAAG GACAAGCTTT TGGCGCCTGA TATGGAATCC 
AAGGTGCGGG TCACCGTTGC GATTACTGCC CTGCTTAACG GTCCATTGGA TGTGGGCAAT 
CAGGTTGTGG CCAGAGAAGG AATACTGCAG ATGATTCTAG CAATGGCTAC GACCGACGAT 
GAGTTGCAGC AGCGAGTATC ATGTGAGTGC CTAATCGCCG CTTCCTCTAA AAAGGACAAG 
GCCAAGGCTC TTTGTGAGCA GGGAGTAGAT ATTTTAAAGC GGCTCTACCA CTCCAAGAAC 
GATGGTATTA GAGTACGCGC CCTGGTGGGT CTTTGCAAGC TGGGTAGCTA CGGCGGTCAG 
GACGCGGCCA TCCGACCATT TGGCGATGGA GCTGCCCTTA AGCTGGCGGA GGCATGCCGT 
CGGTTTTTGA TCAAACCTGG AAAGGATAAG GATATCCGTC GTTGGGCTGC CGATGGTCTG 
GCTTATTTAA CACTGGATGC TGAATGTAAG GAGAAGCTCA TTGAGGATAA GGCTTCCATT 
CACGCTTTAA TGGATTTGGC CCGCGGAGGA AATCAGTCCT GTCTCTATGG TGTGGTCACT 
ACTTTTGTGA ATCTGTGCAA TGCGTATGAG AAGCAGGAGA TGTTGCCTGA GATGATAGAA 
CTTGCCAAGT TTGCTAAGCA ACACATACCG GAGGAGCACG AGCTGGACGA CGTAGATTTC 
ATCAATAAGC GTATTACTGT GCTGGCAAAT GAGGGCATAA CAACTGCTCT TTGTGCACTG 
GCGAAAACTG AAAGCCACAA TTCACAGGAG CTGATTGCCA GGGTTTTGAA TGCGGTTTGT 
GGACTGAAAG AGTTGCGAGG AAAAGTGGTT CAAGAAGGCG GCGTTAAGGC GCTGCTTCGT 
ATGGCGCTTG AGGGAACTGA GAAAGGAAAA CGCCATGCTA CCCAGGCGTT GGCTAGGATT 
GGCATAACCA TTAATCCGGA AGTATCTTTC AGTGGCCAGC GATCGCTTGA TGTAATCCGT 
CCTTTGTTGA ACCTTTTGCA ACAGGACTGC ACAGCGCTGG AGAATTTTGA GTCGCTAATG 
GCACTCACCA ATCTAGCCAG CATGAACGAG AGCGTGCGCC AGAGGATTAT CAAGGAGCAG 
GGGGTCTCCA AGATAGAGTA TTATCTGATG GAGGACCATC TGTACCTGAC TCGTGCAGCT 
GCCCAGTGTC TGTGTAATCT GGTTATGAGT GAGGATGTCA TTAAAATGTT CGAGGGCAAT 
AACGACCGAG TGAAGTTCTT GGCGCTTCTC TGCGAGGATG AGGACGAGGA GACCGCAACA 
GCTTGTGCCG GAGCTCTGGC CATTATTACT TCAGTGTCTG TCAAGTGCTG TGAGAAGATC 
TTGGCCATCG CCAGCTGGCT GGACATTCTG CACACTCTGA TCGCCAATCC CAGTCCGGCG 
GTCCAGCATC GAGGCATTGT GATTATTCTA AACATGATCA ATGCTGGAGA GGAGATCGCC 
AAAAAGCTGT TTGAAACGGA CATTATGGAA CTGCTGAGCG GTTTGGGGCA GCTGCCCGAT 
GACACGCGTG CCAAGGCCCG AGAGGTGGCC ACCCAGTGCC TGGCGGCGGC GGAGCGTTAT 
AGGATCATCG AGCGGTCCGA CAACGCGGAA ATCCCCGATG TGTTTGCCGA AAATTCGAAA 
ATATCTGAGA TTATCGATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

unc-45 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム