nbs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nbs
Entrez Gene ID	44259
Full Name	CG6754 gene product from transcript CG6754-RD
Synonyms	6754,CG6754,Dmel\CG6754,Dnbs1,NBS,NBS1,Nbs,Nbs1,l(3)67BDp,l(3)67BDr,l(3)e77A1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67C5-67C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_143716.5	NP_651973.4	nbs, isoform E
NM_001259773.2	NP_001246702.1	nbs, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identified a stringent requirement for Mre11-Rad50-Nbs (MRN) function in telomere protection during early embryonic development.
Title: Mre11-Rad50-Nbs complex is required to cap telomeres during Drosophila embryogenesis.

This suggests that Nbs and the Mre11/Rad50 complex play partially independent roles in telomere protection and that Nbs functions in both ATR- and ATM-controlled telomere protection pathways.
Title: The Drosophila Nbs protein functions in multiple pathways for the maintenance of genome stability.

The following nbs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nbs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19371	NM_001259773.2 Latest version!	Drosophila melanogaster nbs, transcript variant D (nbs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa20469	NM_143716.5 Latest version!	Drosophila melanogaster nbs, transcript variant E (nbs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nbs ( NM_001259773.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246702.1 Drosophila melanogaster nbs, transcript variant D (nbs), mRNA.

Price & Availability↑

CloneID	OFa19371
Clone ID Related Accession (Same CDS sequence)	NM_001259773.2
Accession Version	NM_001259773.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nbs, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259773.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGTTT TGACTAAAGA TGACGAAAAG TTTGTGTTGT TTCCTGGCAA AAAGGTGTAC 
ACCATTGGTC GCCTGGCCAC GGATTTAATT GTTGCGCAGG ACCTCAGTAT AAGTCGCAAT 
CATGCCCAGC TGCTCATCCA AACGGAAGCG GATGGCGATG ACACCCTGCA TATCGAGGAC 
CTTGGCTCCA GATATGGCAC TTTCATTTTC CCCAAGAATT CCCAAAAGCC GCGCAAGGTG 
CCTGCCAAAA CATCGACTCC ATTGCCTGTG GGCACACGCT TGCGATTTGG CGCAAACATG 
AGCATTTGGC AGGTGACCCA ACTGAAGTTG GTTACAACTG TCTCCGCATT AACGCGTTCC 
GAGGTCCAGG AGCTCACGAA GATGCTTGAA CCCATGGGCG GCACGGTCAC TTCGAACTGG 
ACCGAAGAGT GCTCCCATCT AACCATGAAC GAAGTTTCGG TGACCGTGAA ACTACTGCAC 
GCTATGCTCG AGAATAAGCC GATTGTGACA TTCCCCTACT GGCGAAAGAT GCTGCAGGCA 
GCGCAGAGCA TCCATGTCAA GGAGGGCTGG CCTCAGCCGG AGGATTACCA GCCCACCAAC 
ATAGATGTCA CCTGGCGTCC TGAGCGTACC CGTCTCTTCG CCGGCAAGAC ATTCGTCTTC 
ATGAACCGCA AACACTTTGA TATGTACGGA TCAGTTGTGC AGAAGGCTGG AGCCACGTGC 
AAGGACATCA ATTCGGGTGT GCGCAAAACA TTCCTAACAA AGAGTGATGT GATAGTCATA 
CAGTATGTGC CATCGTCCCA GTCGCAGGCC ACTGAATCAA TCAATAGTAT ACAAGATATT 
TTGGAACAGA ATGGTCGACG TATTATCCAG GAGTACGAGA TTGGCATGGC CCTAATACAT 
TGCTCTATAA CAGAATTCTG CAACCCAACG CACAAGTTCA TCTCCGATTC ATTGCCCACC 
ACGGAGTCTG TAACTTCCTC GATGGCCTTT AACTCTTCGA TCATTGTGCC CAACACGGAA 
CGCCACTCGG CGCAATCCAA TGCCACGCCC ATTTCCGAAC TGGTGGTGCC TGAGTCAATT 
GAATGTGAAA TGGAACAAGA TGCTTCTAAG CCCCATTCGG AGGACCAAGC TTCTTTGCGT 
AAGCGGAGTC ATGCCTCAAC AGTTGACTCC TCGGACGAGG AGAAGAAATC AACATTGTCT 
AAGCGAGCTA AATCGGATAT CGCTACGAAA CTGACCATGA AATCAAAGAA TGCCATATTG 
TTGGACTCGT CTTTGGAAGA AGATGTGACA CCAGCTCCGG CGCCTGCTCC AGTTCAACGA 
GTAACAAGGC AGAGCAAAGC CATCGCTGAG GAGAAATCCG TACATCCACC TGTTCCAGCT 
GCGAGTAAGC ACATAACACG GAAGACCAAA CAGGTTTTTT GTGTTGACTC ATCGGATGAG 
GAGAACGAAA ATGCTCGGAA ACCAAAAGAA ACGCCAGCTC CAACTATCCC TTCGATGGCC 
AAGAAGAAAA CGGAAGCACC CGTTGCCACC AGGATTTCAC CACGACTCAA TGGGAAATCA 
TTGGCCACAA ATATTACCAA CCAACCGGCT GACAAACATG CAGTGCCTGC AAAACGTCCT 
GTTTTATCGG TGGCCTCGAG TGACGAAGAA GATGAAGGGG ACCTATTTCA ATTCCGTAAA 
TCTCCACAGA AGCCAGCAGA GACTGTGGTT CAGCCAAGAA TCGCAGGCAA AGGCAATGCA 
CCTGCCAGAA TCAGTGTAGT CGACTTTCTA GAGAAATCAC AGGCACAAGA ACCAGCTCCA 
GTGCCACCCC AATTGGAGTC CCAATCCCAG ACACAACCGC GCAAGCGTTT ACGCCTTGAG 
CTGCTAAACG AAAGCGACAG CGATGATTGT GATAATCTTT TTAACTTTGC AGACAGCAAA 
AAGAAACGAA AGACGCAAGA GGCTCAGAGA AATGATGACT CCACGGATGG CTTGTTTAAC 
TTCAACTCTG AAAGACCGTC TGATCATGAT GACGAGGATT CCCGGTTAAC CGAACCATTT 
GTACCCGAAA CGGAAAGCAA GAAGCAATCC AAGTACATAG TGGCACCGCG TAGAGATAGA 
CCCAAGAAAG TGGACATCAG TGGTTGGTTA TCGTGTAGCA GGCTAAACGA TAATATCAAA 
TCCGAAATAG ATGCAGACTC AGTTAAGATG GAAACCTCTA TTAAGGCGGA TCCCGATGAG 
GAGCAATGGC TGGCCGCCAT GAAAGACAGC ATTGAGGTGC GCATGTGCAA CTTAAACATT 
GTCATTCGCT CCCAGGAGGA AGTGGATGCT TCTCTTGAGG ATTCTGTGAA CAAGCATGGC 
GGCCGCAAGA ACTTTAAGAA GTTTGTAAAG ACCAAAAATC CTCATCCCCA AAAGCGAATT 
GTTGCGCTTA AGAGTCTTCG GCTGGCTGAT GGTATGGTAA CCTGTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246702.1
CDS	72..2522
Translation	MFVLTKDDEKFVLFPGKKVYTIGRLATDLIVAQDLSISRNHAQLLIQTEADGDDTLHIEDLGSRYGTFIFPKNSQKPRKVPAKTSTPLPVGTRLRFGANMSIWQVTQLKLVTTVSALTRSEVQELTKMLEPMGGTVTSNWTEECSHLTMNEVSVTVKLLHAMLENKPIVTFPYWRKMLQAAQSIHVKEGWPQPEDYQPTNIDVTWRPERTRLFAGKTFVFMNRKHFDMYGSVVQKAGATCKDINSGVRKTFLTKSDVIVIQYVPSSQSQATESINSIQDILEQNGRRIIQEYEIGMALIHCSITEFCNPTHKFISDSLPTTESVTSSMAFNSSIIVPNTERHSAQSNATPISELVVPESIECEMEQDASKPHSEDQASLRKRSHASTVDSSDEEKKSTLSKRAKSDIATKLTMKSKNAILLDSSLEEDVTPAPAPAPVQRVTRQSKAIAEEKSVHPPVPAASKHITRKTKQVFCVDSSDEENENARKPKETPAPTIPSMAKKKTEAPVATRISPRLNGKSLATNITNQPADKHAVPAKRPVLSVASSDEEDEGDLFQFRKSPQKPAETVVQPRIAGKGNAPARISVVDFLEKSQAQEPAPVPPQLESQSQTQPRKRLRLELLNESDSDDCDNLFNFADSKKKRKTQEAQRNDDSTDGLFNFNSERPSDHDDEDSRLTEPFVPETESKKQSKYIVAPRRDRPKKVDISGWLSCSRLNDNIKSEIDADSVKMETSIKADPDEEQWLAAMKDSIEVRMCNLNIVIRSQEEVDASLEDSVNKHGGRKNFKKFVKTKNPHPQKRIVALKSLRLADGMVTCV

Target ORF information:

RefSeq Version	NM_001259773.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nbs, transcript variant D (nbs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259773.2

ORF Insert Sequence:

ATGTTTGTTT TGACTAAAGA TGACGAAAAG TTTGTGTTGT TTCCTGGCAA AAAGGTGTAC 
ACCATTGGTC GCCTGGCCAC GGATTTAATT GTTGCGCAGG ACCTCAGTAT AAGTCGCAAT 
CATGCCCAGC TGCTCATCCA AACGGAAGCG GATGGCGATG ACACCCTGCA TATCGAGGAC 
CTTGGCTCCA GATATGGCAC TTTCATTTTC CCCAAGAATT CCCAAAAGCC GCGCAAGGTG 
CCTGCCAAAA CATCGACTCC ATTGCCTGTG GGCACACGCT TGCGATTTGG CGCAAACATG 
AGCATTTGGC AGGTGACCCA ACTGAAGTTG GTTACAACTG TCTCCGCATT AACGCGTTCC 
GAGGTCCAGG AGCTCACGAA GATGCTTGAA CCCATGGGCG GCACGGTCAC TTCGAACTGG 
ACCGAAGAGT GCTCCCATCT AACCATGAAC GAAGTTTCGG TGACCGTGAA ACTACTGCAC 
GCTATGCTCG AGAATAAGCC GATTGTGACA TTCCCCTACT GGCGAAAGAT GCTGCAGGCA 
GCGCAGAGCA TCCATGTCAA GGAGGGCTGG CCTCAGCCGG AGGATTACCA GCCCACCAAC 
ATAGATGTCA CCTGGCGTCC TGAGCGTACC CGTCTCTTCG CCGGCAAGAC ATTCGTCTTC 
ATGAACCGCA AACACTTTGA TATGTACGGA TCAGTTGTGC AGAAGGCTGG AGCCACGTGC 
AAGGACATCA ATTCGGGTGT GCGCAAAACA TTCCTAACAA AGAGTGATGT GATAGTCATA 
CAGTATGTGC CATCGTCCCA GTCGCAGGCC ACTGAATCAA TCAATAGTAT ACAAGATATT 
TTGGAACAGA ATGGTCGACG TATTATCCAG GAGTACGAGA TTGGCATGGC CCTAATACAT 
TGCTCTATAA CAGAATTCTG CAACCCAACG CACAAGTTCA TCTCCGATTC ATTGCCCACC 
ACGGAGTCTG TAACTTCCTC GATGGCCTTT AACTCTTCGA TCATTGTGCC CAACACGGAA 
CGCCACTCGG CGCAATCCAA TGCCACGCCC ATTTCCGAAC TGGTGGTGCC TGAGTCAATT 
GAATGTGAAA TGGAACAAGA TGCTTCTAAG CCCCATTCGG AGGACCAAGC TTCTTTGCGT 
AAGCGGAGTC ATGCCTCAAC AGTTGACTCC TCGGACGAGG AGAAGAAATC AACATTGTCT 
AAGCGAGCTA AATCGGATAT CGCTACGAAA CTGACCATGA AATCAAAGAA TGCCATATTG 
TTGGACTCGT CTTTGGAAGA AGATGTGACA CCAGCTCCGG CGCCTGCTCC AGTTCAACGA 
GTAACAAGGC AGAGCAAAGC CATCGCTGAG GAGAAATCCG TACATCCACC TGTTCCAGCT 
GCGAGTAAGC ACATAACACG GAAGACCAAA CAGGTTTTTT GTGTTGACTC ATCGGATGAG 
GAGAACGAAA ATGCTCGGAA ACCAAAAGAA ACGCCAGCTC CAACTATCCC TTCGATGGCC 
AAGAAGAAAA CGGAAGCACC CGTTGCCACC AGGATTTCAC CACGACTCAA TGGGAAATCA 
TTGGCCACAA ATATTACCAA CCAACCGGCT GACAAACATG CAGTGCCTGC AAAACGTCCT 
GTTTTATCGG TGGCCTCGAG TGACGAAGAA GATGAAGGGG ACCTATTTCA ATTCCGTAAA 
TCTCCACAGA AGCCAGCAGA GACTGTGGTT CAGCCAAGAA TCGCAGGCAA AGGCAATGCA 
CCTGCCAGAA TCAGTGTAGT CGACTTTCTA GAGAAATCAC AGGCACAAGA ACCAGCTCCA 
GTGCCACCCC AATTGGAGTC CCAATCCCAG ACACAACCGC GCAAGCGTTT ACGCCTTGAG 
CTGCTAAACG AAAGCGACAG CGATGATTGT GATAATCTTT TTAACTTTGC AGACAGCAAA 
AAGAAACGAA AGACGCAAGA GGCTCAGAGA AATGATGACT CCACGGATGG CTTGTTTAAC 
TTCAACTCTG AAAGACCGTC TGATCATGAT GACGAGGATT CCCGGTTAAC CGAACCATTT 
GTACCCGAAA CGGAAAGCAA GAAGCAATCC AAGTACATAG TGGCACCGCG TAGAGATAGA 
CCCAAGAAAG TGGACATCAG TGGTTGGTTA TCGTGTAGCA GGCTAAACGA TAATATCAAA 
TCCGAAATAG ATGCAGACTC AGTTAAGATG GAAACCTCTA TTAAGGCGGA TCCCGATGAG 
GAGCAATGGC TGGCCGCCAT GAAAGACAGC ATTGAGGTGC GCATGTGCAA CTTAAACATT 
GTCATTCGCT CCCAGGAGGA AGTGGATGCT TCTCTTGAGG ATTCTGTGAA CAAGCATGGC 
GGCCGCAAGA ACTTTAAGAA GTTTGTAAAG ACCAAAAATC CTCATCCCCA AAAGCGAATT 
GTTGCGCTTA AGAGTCTTCG GCTGGCTGAT GGTATGGTAA CCTGTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nbs ( NM_143716.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651973.4 Drosophila melanogaster nbs, transcript variant E (nbs), mRNA.

Price & Availability↑

CloneID	OFa20469
Clone ID Related Accession (Same CDS sequence)	NM_143716.5
Accession Version	NM_143716.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nbs, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_143716.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGTTT TGACTAAAGA TGACGAAAAG TTTGTGTTGT TTCCTGGCAA AAAGGTGTAC 
ACCATTGGTC GCCTGGCCAC GGATTTAATT GTTGCGCAGG ACCTCAGTAT AAGTCGCAAT 
CATGCCCAGC TGCTCATCCA AACGGAAGCG GATGGCGATG ACACCCTGCA TATCGAGGAC 
CTTGGCTCCA GATATGGCAC TTTCATTTTC CCCAAGAATT CCCAAAAGCC GCGCAAGGTG 
CCTGCCAAAA CATCGACTCC ATTGCCTGTG GGCACACGCT TGCGATTTGG CGCAAACATG 
AGCATTTGGC AGGTGACCCA ACTGAAGTTG GTTACAACTG TCTCCGCATT AACGCGTTCC 
GAGGTCCAGG AGCTCACGAA GATGCTTGAA CCCATGGGCG GCACGGTCAC TTCGAACTGG 
ACCGAAGAGT GCTCCCATCT AACCATGAAC GAAGTTTCGG TGACCGTGAA ACTACTGCAC 
GCTATGCTCG AGAATAAGCC GATTGTGACA TTCCCCTACT GGCGAAAGAT GCTGCAGGCA 
GCGCAGAGCA TCCATGTCAA GGAGGGCTGG CCTCAGCCGG AGGATTACCA GCCCACCAAC 
ATAGATGTCA CCTGGCGTCC TGAGCGTACC CGTCTCTTCG CCGGCAAGAC ATTCGTCTTC 
ATGAACCGCA AACACTTTGA TATGTACGGA TCAGTTGTGC AGAAGGCTGG AGCCACGTGC 
AAGGACATCA ATTCGGGTGT GCGCAAAACA TTCCTAACAA AGAGTGATGT GATAGTCATA 
CAGTATGTGC CATCGTCCCA GTCGCAGGCC ACTGAATCAA TCAATAGTAT ACAAGATAGG 
TATATTTTGG AACAGAATGG TCGACGTATT ATCCAGGAGT ACGAGATTGG CATGGCCCTA 
ATACATTGCT CTATAACAGA ATTCTGCAAC CCAACGCACA AGTTCATCTC CGATTCATTG 
CCCACCACGG AGTCTGTAAC TTCCTCGATG GCCTTTAACT CTTCGATCAT TGTGCCCAAC 
ACGGAACGCC ACTCGGCGCA ATCCAATGCC ACGCCCATTT CCGAACTGGT GGTGCCTGAG 
TCAATTGAAT GTGAAATGGA ACAAGATGCT TCTAAGCCCC ATTCGGAGGA CCAAGCTTCT 
TTGCGTAAGC GGAGTCATGC CTCAACAGTT GACTCCTCGG ACGAGGAGAA GAAATCAACA 
TTGTCTAAGC GAGCTAAATC GGATATCGCT ACGAAACTGA CCATGAAATC AAAGAATGCC 
ATATTGTTGG ACTCGTCTTT GGAAGAAGAT GTGACACCAG CTCCGGCGCC TGCTCCAGTT 
CAACGAGTAA CAAGGCAGAG CAAAGCCATC GCTGAGGAGA AATCCGTACA TCCACCTGTT 
CCAGCTGCGA GTAAGCACAT AACACGGAAG ACCAAACAGG TTTTTTGTGT TGACTCATCG 
GATGAGGAGA ACGAAAATGC TCGGAAACCA AAAGAAACGC CAGCTCCAAC TATCCCTTCG 
ATGGCCAAGA AGAAAACGGA AGCACCCGTT GCCACCAGGA TTTCACCACG ACTCAATGGG 
AAATCATTGG CCACAAATAT TACCAACCAA CCGGCTGACA AACATGCAGT GCCTGCAAAA 
CGTCCTGTTT TATCGGTGGC CTCGAGTGAC GAAGAAGATG AAGGGGACCT ATTTCAATTC 
CGTAAATCTC CACAGAAGCC AGCAGAGACT GTGGTTCAGC CAAGAATCGC AGGCAAAGGC 
AATGCACCTG CCAGAATCAG TGTAGTCGAC TTTCTAGAGA AATCACAGGC ACAAGAACCA 
GCTCCAGTGC CACCCCAATT GGAGTCCCAA TCCCAGACAC AACCGCGCAA GCGTTTACGC 
CTTGAGCTGC TAAACGAAAG CGACAGCGAT GATTGTGATA ATCTTTTTAA CTTTGCAGAC 
AGCAAAAAGA AACGAAAGAC GCAAGAGGCT CAGAGAAATG ATGACTCCAC GGATGGCTTG 
TTTAACTTCA ACTCTGAAAG ACCGTCTGAT CATGATGACG AGGATTCCCG GTTAACCGAA 
CCATTTGTAC CCGAAACGGA AAGCAAGAAG CAATCCAAGT ACATAGTGGC ACCGCGTAGA 
GATAGACCCA AGAAAGTGGA CATCAGTGGT TGGTTATCGT GTAGCAGGCT AAACGATAAT 
ATCAAATCCG AAATAGATGC AGACTCAGTT AAGATGGAAA CCTCTATTAA GGCGGATCCC 
GATGAGGAGC AATGGCTGGC CGCCATGAAA GACAGCATTG AGGTGCGCAT GTGCAACTTA 
AACATTGTCA TTCGCTCCCA GGAGGAAGTG GATGCTTCTC TTGAGGATTC TGTGAACAAG 
CATGGCGGCC GCAAGAACTT TAAGAAGTTT GTAAAGACCA AAAATCCTCA TCCCCAAAAG 
CGAATTGTTG CGCTTAAGAG TCTTCGGCTG GCTGATGGTA TGGTAACCTG TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651973.4
CDS	72..2528
Translation	MFVLTKDDEKFVLFPGKKVYTIGRLATDLIVAQDLSISRNHAQLLIQTEADGDDTLHIEDLGSRYGTFIFPKNSQKPRKVPAKTSTPLPVGTRLRFGANMSIWQVTQLKLVTTVSALTRSEVQELTKMLEPMGGTVTSNWTEECSHLTMNEVSVTVKLLHAMLENKPIVTFPYWRKMLQAAQSIHVKEGWPQPEDYQPTNIDVTWRPERTRLFAGKTFVFMNRKHFDMYGSVVQKAGATCKDINSGVRKTFLTKSDVIVIQYVPSSQSQATESINSIQDRYILEQNGRRIIQEYEIGMALIHCSITEFCNPTHKFISDSLPTTESVTSSMAFNSSIIVPNTERHSAQSNATPISELVVPESIECEMEQDASKPHSEDQASLRKRSHASTVDSSDEEKKSTLSKRAKSDIATKLTMKSKNAILLDSSLEEDVTPAPAPAPVQRVTRQSKAIAEEKSVHPPVPAASKHITRKTKQVFCVDSSDEENENARKPKETPAPTIPSMAKKKTEAPVATRISPRLNGKSLATNITNQPADKHAVPAKRPVLSVASSDEEDEGDLFQFRKSPQKPAETVVQPRIAGKGNAPARISVVDFLEKSQAQEPAPVPPQLESQSQTQPRKRLRLELLNESDSDDCDNLFNFADSKKKRKTQEAQRNDDSTDGLFNFNSERPSDHDDEDSRLTEPFVPETESKKQSKYIVAPRRDRPKKVDISGWLSCSRLNDNIKSEIDADSVKMETSIKADPDEEQWLAAMKDSIEVRMCNLNIVIRSQEEVDASLEDSVNKHGGRKNFKKFVKTKNPHPQKRIVALKSLRLADGMVTCV

Target ORF information:

RefSeq Version	NM_143716.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nbs, transcript variant E (nbs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143716.5

ORF Insert Sequence:

ATGTTTGTTT TGACTAAAGA TGACGAAAAG TTTGTGTTGT TTCCTGGCAA AAAGGTGTAC 
ACCATTGGTC GCCTGGCCAC GGATTTAATT GTTGCGCAGG ACCTCAGTAT AAGTCGCAAT 
CATGCCCAGC TGCTCATCCA AACGGAAGCG GATGGCGATG ACACCCTGCA TATCGAGGAC 
CTTGGCTCCA GATATGGCAC TTTCATTTTC CCCAAGAATT CCCAAAAGCC GCGCAAGGTG 
CCTGCCAAAA CATCGACTCC ATTGCCTGTG GGCACACGCT TGCGATTTGG CGCAAACATG 
AGCATTTGGC AGGTGACCCA ACTGAAGTTG GTTACAACTG TCTCCGCATT AACGCGTTCC 
GAGGTCCAGG AGCTCACGAA GATGCTTGAA CCCATGGGCG GCACGGTCAC TTCGAACTGG 
ACCGAAGAGT GCTCCCATCT AACCATGAAC GAAGTTTCGG TGACCGTGAA ACTACTGCAC 
GCTATGCTCG AGAATAAGCC GATTGTGACA TTCCCCTACT GGCGAAAGAT GCTGCAGGCA 
GCGCAGAGCA TCCATGTCAA GGAGGGCTGG CCTCAGCCGG AGGATTACCA GCCCACCAAC 
ATAGATGTCA CCTGGCGTCC TGAGCGTACC CGTCTCTTCG CCGGCAAGAC ATTCGTCTTC 
ATGAACCGCA AACACTTTGA TATGTACGGA TCAGTTGTGC AGAAGGCTGG AGCCACGTGC 
AAGGACATCA ATTCGGGTGT GCGCAAAACA TTCCTAACAA AGAGTGATGT GATAGTCATA 
CAGTATGTGC CATCGTCCCA GTCGCAGGCC ACTGAATCAA TCAATAGTAT ACAAGATAGG 
TATATTTTGG AACAGAATGG TCGACGTATT ATCCAGGAGT ACGAGATTGG CATGGCCCTA 
ATACATTGCT CTATAACAGA ATTCTGCAAC CCAACGCACA AGTTCATCTC CGATTCATTG 
CCCACCACGG AGTCTGTAAC TTCCTCGATG GCCTTTAACT CTTCGATCAT TGTGCCCAAC 
ACGGAACGCC ACTCGGCGCA ATCCAATGCC ACGCCCATTT CCGAACTGGT GGTGCCTGAG 
TCAATTGAAT GTGAAATGGA ACAAGATGCT TCTAAGCCCC ATTCGGAGGA CCAAGCTTCT 
TTGCGTAAGC GGAGTCATGC CTCAACAGTT GACTCCTCGG ACGAGGAGAA GAAATCAACA 
TTGTCTAAGC GAGCTAAATC GGATATCGCT ACGAAACTGA CCATGAAATC AAAGAATGCC 
ATATTGTTGG ACTCGTCTTT GGAAGAAGAT GTGACACCAG CTCCGGCGCC TGCTCCAGTT 
CAACGAGTAA CAAGGCAGAG CAAAGCCATC GCTGAGGAGA AATCCGTACA TCCACCTGTT 
CCAGCTGCGA GTAAGCACAT AACACGGAAG ACCAAACAGG TTTTTTGTGT TGACTCATCG 
GATGAGGAGA ACGAAAATGC TCGGAAACCA AAAGAAACGC CAGCTCCAAC TATCCCTTCG 
ATGGCCAAGA AGAAAACGGA AGCACCCGTT GCCACCAGGA TTTCACCACG ACTCAATGGG 
AAATCATTGG CCACAAATAT TACCAACCAA CCGGCTGACA AACATGCAGT GCCTGCAAAA 
CGTCCTGTTT TATCGGTGGC CTCGAGTGAC GAAGAAGATG AAGGGGACCT ATTTCAATTC 
CGTAAATCTC CACAGAAGCC AGCAGAGACT GTGGTTCAGC CAAGAATCGC AGGCAAAGGC 
AATGCACCTG CCAGAATCAG TGTAGTCGAC TTTCTAGAGA AATCACAGGC ACAAGAACCA 
GCTCCAGTGC CACCCCAATT GGAGTCCCAA TCCCAGACAC AACCGCGCAA GCGTTTACGC 
CTTGAGCTGC TAAACGAAAG CGACAGCGAT GATTGTGATA ATCTTTTTAA CTTTGCAGAC 
AGCAAAAAGA AACGAAAGAC GCAAGAGGCT CAGAGAAATG ATGACTCCAC GGATGGCTTG 
TTTAACTTCA ACTCTGAAAG ACCGTCTGAT CATGATGACG AGGATTCCCG GTTAACCGAA 
CCATTTGTAC CCGAAACGGA AAGCAAGAAG CAATCCAAGT ACATAGTGGC ACCGCGTAGA 
GATAGACCCA AGAAAGTGGA CATCAGTGGT TGGTTATCGT GTAGCAGGCT AAACGATAAT 
ATCAAATCCG AAATAGATGC AGACTCAGTT AAGATGGAAA CCTCTATTAA GGCGGATCCC 
GATGAGGAGC AATGGCTGGC CGCCATGAAA GACAGCATTG AGGTGCGCAT GTGCAACTTA 
AACATTGTCA TTCGCTCCCA GGAGGAAGTG GATGCTTCTC TTGAGGATTC TGTGAACAAG 
CATGGCGGCC GCAAGAACTT TAAGAAGTTT GTAAAGACCA AAAATCCTCA TCCCCAAAAG 
CGAATTGTTG CGCTTAAGAG TCTTCGGCTG GCTGATGGTA TGGTAACCTG TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nbs cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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