wake cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wake
Entrez Gene ID	42676
Full Name	wide awake
Synonyms	CG17622,CG17623,CG42686,CG45058,CG6954,Dmel\CG45058,Dmel_CG17622,Dmel_CG17623,Dmel_CG42686,Dmel_CG6954,ban
General protein information	Preferred Names wide awake Names CG45058 gene product from transcript CG45058-RH CG45058-PB CG45058-PC CG45058-PD CG45058-PE CG45058-PF CG45058-PG CG45058-PH banderuola wake-PB wake-PC wake-PD wake-PE wake-PF wake-PG wake-PH
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94B8-94B11

mRNA and Protein(s)

mRNA	Protein	Name
NM_142819.3	NP_651076.2	wide awake, isoform F
NM_001260316.1	NP_001247245.1	wide awake, isoform E
NM_001300540.1	NP_001287469.1	wide awake, isoform G
NM_001300541.1	NP_001287470.1	wide awake, isoform H
NM_001202337.2	NP_001189266.1	wide awake, isoform B
NM_001260314.3	NP_001247243.1	wide awake, isoform C
NM_001260315.1	NP_001247244.1	wide awake, isoform D

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ANKFN1	XP_511898.4
Danio rerio (zebrafish)	LOC100333998	XP_003201826.2
Danio rerio (zebrafish)	LOC557615	XP_003199674.2
Drosophila melanogaster (fruit fly)	CG6954	NP_001247244.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001726	XP_321358.5
	LOC707235	XP_002808169.1
Homo sapiens (human)	ANKFN1	NP_694960.2
Canis lupus familiaris (dog)	ANKFN1	XP_548219.3
Bos taurus (cattle)	ANKFN1	XP_003587422.2
Mus musculus (house mouse)	Ankfn1	NP_001074402.1
Gallus gallus (chicken)	ANKFN1	XP_004946219.1
Xenopus tropicalis (tropical clawed frog)	LOC100490095	XP_004918819.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

homozygotes for null mutations in the Drosophila homolog of Nmf9, CG45058, show profound locomotor defects and premature death, while heterozygotes show striking effects on sleep and activity phenotypes.
Title: Nmf9 Encodes a Highly Conserved Protein Important to Neurological Function in Mice and Flies.

The following wake gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wake cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05720	NM_142819.3 Latest version!	Drosophila melanogaster wide awake (wake), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa05720	NM_001202337.2 Latest version!	Drosophila melanogaster wide awake (wake), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa05719	NM_001260316.1 Latest version!	Drosophila melanogaster wide awake (wake), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa05719	NM_001260315.1 Latest version!	Drosophila melanogaster wide awake (wake), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa05721	NM_001260314.3 Latest version!	Drosophila melanogaster wide awake (wake), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa22136	NM_001300541.1 Latest version!	Drosophila melanogaster wide awake (wake), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa22137	NM_001300540.1 Latest version!	Drosophila melanogaster wide awake (wake), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wake ( NM_142819.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651076.2 Drosophila melanogaster wide awake (wake), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa05720
Clone ID Related Accession (Same CDS sequence)	NM_142819.3 , NM_001202337.2
Accession Version	NM_142819.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wide awake, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142819.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATTCC TACTGCCCCA TTCGCGAACC GATTCCCAAT CGAATTCCCA ATCGCAACTG 
TGCATTTCGC GGCACTCGCA GCCGAAAAAT GCCAAGCTGC GGGATGATCA CGATGAGACG 
GGCTCATCGG AATTGGATTC ACCACCGCTG GGTGATGAGA ATGCCTCGGC GAATGGCCAA 
CTTTCGGTGC ACAGGCAGCC GGTACAGCTG CGGAGTAAGA TCCCGGGAAA CACACCTCAT 
CCGCGGGCCA GCAAAATGTC CAAGAAACAG CTGAAGTTGG CCCAGGCCCA GCTGGACAAA 
CTGACGCAGA ACAATCTGCA TCTGCATGCT CTCTTCTCGG CAGTGGAGCA TGGTCACCTG 
GAGAAGGCGC GCACTATTCT AGAGTCCACC GACGTAGATG TAAACAGCAT CAATAACGAT 
GGACTATCTG CTCTGGACTT GGCGGTGTTA AGCAATAATC GCTCCATGAC CAGGATGCTG 
CTTCAGCATG GCGCTGTGGA GGGCTCTCAG TTCTCTGTGG ACACCATTGG CACCAAACTG 
AACGGCTTGC TCAAGGACGC CGAGTCAAGA ATTCACGATC TAAGTGGACC GGAGGGCCTC 
TGTCCGCCGG TGTTTGCATC GCGTCCCTCC ATCTCGAGCA TCATAATTGG CAATTCCAGT 
GCATCGGTAA CTGGCTGCAC GGGCAGCGAG GTGGAAAAAC AGATAGGCAT CTGGGAGCGC 
CGAGTTAAGG GACTGCGTCG CTTGCAGCTC GGTTGGGATC AGGCCAGGCC GCCAGATGCA 
CCCGCCTCCG TGGTCGTCGA CGTCACTGGC GACAATTCCA TCAGCGTACA AATCCTAGAG 
CCCTTCGAGG GGGCCATCGG CACGAAATTT AAAGTACAAT GGTCGACCCG CGCGGATTTC 
AACAACGTTG TGGGCGAGAG TGAGCTACTG GAATGGATCA GCTTCCATGG CACGATGGGT 
GCCCAGTGTC ACATATCGGG CCTCACCCAG GGTCGTCGCT ACTTCCTGCG AGCCGCGTGC 
GGCAATGTGA AGGGATGGGG CACCTATCGA ACCTCTGTGC CGGCTAGTGT AGTGCCCTCA 
ACTTGGCGGG ATTTAGACAA TCGAGAGGAC CGATTCGTGG GTCGTCACCG AATCCTGGAC 
AATCTATTTA CCGCCGTGCG TTTGGCAAGA CCCGCCGACG TATCCGAGCT GACTTTGGAT 
CCTGCGAGCG CCCAACGACG TAATCCCAAA AAGAAGACTA CCATTAAGCA GTTGTTCTCG 
GTAACCACCA AGTTTCAAAA GACACTGAGA CGCGGCATTT ACTTCTCTTG TATTATTCAT 
TGCGATGATA AAGTGCTGGT CACCAGCGAA GACTTTCCAC CGGTCATCGA GGTGGATGAA 
TCCTATCCGA GTGCTTTGCA CATGGATTAC TACTGGCTCA TGAAAGTTGC CTGCACCTGG 
GAGGATGTTA AGTCTCTACG TTCGGATATG GAGCGCAATC TTACCTCCGC TGTTCACTTC 
CGCACTAAGC TTCTATCGGC GGTCTGTCAG ATGCAATCGG CACTAGGCAT TACGGACTTG 
GGCCAACTTT ACTATAAACC ATTGCGGGAT GCTCAGGGCA CTGTGGTCCT AACCTGTGTG 
CAGTCGGTAA AGAGCCAAAA GGCCGTCTCC ATCTTAAACT CGAGATGGGT TCCAGTCAGC 
AAGCTGCAAA AGAAGCTAGG TGCTCTACAC GAGGACTACA CCATTAACGA GCTGCTCATC 
TCCTCGATTG GAGATCAATT GCACTATCAA CAGGCGGCGC TGCAGCGTTT GGAGCCCGGT 
CTTTACCTGG GCTACCTAAA GATGCAATGC TCCATAGACC AGATCCAGGT GGTTGTGCCC 
GTGAAAACGC CGAATGTCTT GCCCCACTGC AAGGTGCGCG AAAACAGTCA CATCACGGCC 
GAAGAGTGGC AGGTGCTCCA CCGTTACAGT AGCGATCCTC TACGCCTGCC GTTGGACTTT 
AGCGCTCAAG GAGGAGATGG AGCCTCCGGA GCAACAGCTA CTACGGAGGT GCAACGCCTA 
TTTTTATACG ATCTCACCAA TGCAATGCAC AAACTGTTTG CCAGTATGAA CATCAAAATC 
GCTGATGCAA CCACTCACCG CTTGTACGAC GTGGAGGTGA TTGAGCATAG TCCGGATATT 
AGTTTCCTCG TGGTGTGTCC ATCCGCCGAG TCCTCATGCG CTGTGCCCGG TCAGTCTGAG 
TTACTGCTAC AGCGAGATGA CTTGGCCAGC TTGAGTATCC AGGCCTTTGA GATGATTCAC 
CTGCGCACTT ATCAGCCGGC CATCATTCAG AAGTACGCCC GTTTATCTTG CATCCTTGAA 
TTGGATACCG CACTGGCCAC ACATTCGCTT CGCGAGGCAT TCTCCAGCAG TGAACTGCAG 
GCGGCCAAGG AACGTTTGGC CACGCTGCAG GAGCTCAGTG CGAGTCTTAC GATTGTGTGG 
AAAAGTGTGC GATGGCTAAT GGATGTGGTG GCATATGCGC GAAACAAAAA CGCTCAACCC 
TCGTTGGCTA TGCGAGAAAT TCTGGACTTT GCACAGCAAA GACAGGACGA AGCGGTAGCG 
ACATCGGCAG GAGGTTCCGC GAACAAACAG CTGCTGCAGC TGCCCATCCG CGAGAGCAAG 
TTCTCCAAGA CGGGCCAGGG AAGAGGCAGT TGGCCGGGAC CGGGAACTAG TGAAGATCAA 
TCGCAGAACA AGCCGGAGCA CTCCAAGTCT GAACAGAATC TGGAACTGAG TGCTATCACG 
CCAGTGGAAC CTGCATCCAA TCAAGTTCCT ACACAGCAGC AGCAGCCGCA GCAGCAATCG 
CAGCAACAGC AACTCTTACA AGTTTCCAAC ATCAGCGAAT ACGCGGGATC GATTTGCTCA 
GAAGTATCAT TTAGGAAGAA TAGTGGCGAT TCCATGAGCT CCACCTACAC GTCCCGGAGC 
TTCTACTCTG CCGTGGACTC AGCCTCAGAT GGAAATAGCA CAAACAGTGT GTTTGCCATT 
CCGCCCTCTC GTTCCGACGA CACTCTGGCG GATGCACTGC GCCACTCGCA AGCGGTAGCT 
GCGCAACGCA AACGAACCAG CTCTAATATC GCATCTCATA CCAATCCCTT GATTACGGTG 
CACAGCTCTA GTTCGGCTCC GTACCTGGCT GGATCCAGTG TTTCCTTGAG AAGCGGCAAC 
GGAGCCTTCG CAACGGATTT GGAGCACAAA CCGCTGAAGC CAGCGACAAA GGCTGCATCT 
AGTGCAAACC TGCGCGAAGG TGGACCCTAT TTGAAAAGCA CGTTAGCTGA ACTACGAGCT 
GTTGGAGGTG AGGAGCCGGT GGCCAGTACT TCAAAGGCCT CTTCGATGAA GAGCTTGTCG 
CGGCAGAGCA GCGAAGAGGA TTCCGCCAGC TGTTCCAGTC TCAACGCAGA GCAGACATCG 
GGGATCATTC AAGTCTATAC CGCCTACAGT ACGGGTCTGG CCAGTGGAAC CAGCTTAAAG 
ATTCACGTAA CTCCGAAGAC GACTGCCCGC GAAGTGATCA ACCTGGTGGT GAAGCAACTT 
AACATGGCCG TGGTTCTCAA AGGGAACAAT GGCCCCATCT ATGGACCCGA GATGCTAGAG 
AACTTTTGCC TGGTTGCGGT GATTGGAGCA AGGGAACGCT GCCTGCGAGA TGACTTCAAA 
CCGCTGCAAC TGCAGAATCC CTGGAAGAAG GGCCGTCTGT ACGTCCGCAA GAAGCATGAG 
CTGCTGGCGG CCATCGAACA CTCGAATCGG AAATCGCATT TGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651076.2
CDS	609..4376
Translation	MEFLLPHSRTDSQSNSQSQLCISRHSQPKNAKLRDDHDETGSSELDSPPLGDENASANGQLSVHRQPVQLRSKIPGNTPHPRASKMSKKQLKLAQAQLDKLTQNNLHLHALFSAVEHGHLEKARTILESTDVDVNSINNDGLSALDLAVLSNNRSMTRMLLQHGAVEGSQFSVDTIGTKLNGLLKDAESRIHDLSGPEGLCPPVFASRPSISSIIIGNSSASVTGCTGSEVEKQIGIWERRVKGLRRLQLGWDQARPPDAPASVVVDVTGDNSISVQILEPFEGAIGTKFKVQWSTRADFNNVVGESELLEWISFHGTMGAQCHISGLTQGRRYFLRAACGNVKGWGTYRTSVPASVVPSTWRDLDNREDRFVGRHRILDNLFTAVRLARPADVSELTLDPASAQRRNPKKKTTIKQLFSVTTKFQKTLRRGIYFSCIIHCDDKVLVTSEDFPPVIEVDESYPSALHMDYYWLMKVACTWEDVKSLRSDMERNLTSAVHFRTKLLSAVCQMQSALGITDLGQLYYKPLRDAQGTVVLTCVQSVKSQKAVSILNSRWVPVSKLQKKLGALHEDYTINELLISSIGDQLHYQQAALQRLEPGLYLGYLKMQCSIDQIQVVVPVKTPNVLPHCKVRENSHITAEEWQVLHRYSSDPLRLPLDFSAQGGDGASGATATTEVQRLFLYDLTNAMHKLFASMNIKIADATTHRLYDVEVIEHSPDISFLVVCPSAESSCAVPGQSELLLQRDDLASLSIQAFEMIHLRTYQPAIIQKYARLSCILELDTALATHSLREAFSSSELQAAKERLATLQELSASLTIVWKSVRWLMDVVAYARNKNAQPSLAMREILDFAQQRQDEAVATSAGGSANKQLLQLPIRESKFSKTGQGRGSWPGPGTSEDQSQNKPEHSKSEQNLELSAITPVEPASNQVPTQQQQPQQQSQQQQLLQVSNISEYAGSICSEVSFRKNSGDSMSSTYTSRSFYSAVDSASDGNSTNSVFAIPPSRSDDTLADALRHSQAVAAQRKRTSSNIASHTNPLITVHSSSSAPYLAGSSVSLRSGNGAFATDLEHKPLKPATKAASSANLREGGPYLKSTLAELRAVGGEEPVASTSKASSMKSLSRQSSEEDSASCSSLNAEQTSGIIQVYTAYSTGLASGTSLKIHVTPKTTAREVINLVVKQLNMAVVLKGNNGPIYGPEMLENFCLVAVIGARERCLRDDFKPLQLQNPWKKGRLYVRKKHELLAAIEHSNRKSHLI

Target ORF information:

RefSeq Version	NM_142819.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wide awake (wake), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142819.3

ORF Insert Sequence:

ATGGAATTCC TACTGCCCCA TTCGCGAACC GATTCCCAAT CGAATTCCCA ATCGCAACTG 
TGCATTTCGC GGCACTCGCA GCCGAAAAAT GCCAAGCTGC GGGATGATCA CGATGAGACG 
GGCTCATCGG AATTGGATTC ACCACCGCTG GGTGATGAGA ATGCCTCGGC GAATGGCCAA 
CTTTCGGTGC ACAGGCAGCC GGTACAGCTG CGGAGTAAGA TCCCGGGAAA CACACCTCAT 
CCGCGGGCCA GCAAAATGTC CAAGAAACAG CTGAAGTTGG CCCAGGCCCA GCTGGACAAA 
CTGACGCAGA ACAATCTGCA TCTGCATGCT CTCTTCTCGG CAGTGGAGCA TGGTCACCTG 
GAGAAGGCGC GCACTATTCT AGAGTCCACC GACGTAGATG TAAACAGCAT CAATAACGAT 
GGACTATCTG CTCTGGACTT GGCGGTGTTA AGCAATAATC GCTCCATGAC CAGGATGCTG 
CTTCAGCATG GCGCTGTGGA GGGCTCTCAG TTCTCTGTGG ACACCATTGG CACCAAACTG 
AACGGCTTGC TCAAGGACGC CGAGTCAAGA ATTCACGATC TAAGTGGACC GGAGGGCCTC 
TGTCCGCCGG TGTTTGCATC GCGTCCCTCC ATCTCGAGCA TCATAATTGG CAATTCCAGT 
GCATCGGTAA CTGGCTGCAC GGGCAGCGAG GTGGAAAAAC AGATAGGCAT CTGGGAGCGC 
CGAGTTAAGG GACTGCGTCG CTTGCAGCTC GGTTGGGATC AGGCCAGGCC GCCAGATGCA 
CCCGCCTCCG TGGTCGTCGA CGTCACTGGC GACAATTCCA TCAGCGTACA AATCCTAGAG 
CCCTTCGAGG GGGCCATCGG CACGAAATTT AAAGTACAAT GGTCGACCCG CGCGGATTTC 
AACAACGTTG TGGGCGAGAG TGAGCTACTG GAATGGATCA GCTTCCATGG CACGATGGGT 
GCCCAGTGTC ACATATCGGG CCTCACCCAG GGTCGTCGCT ACTTCCTGCG AGCCGCGTGC 
GGCAATGTGA AGGGATGGGG CACCTATCGA ACCTCTGTGC CGGCTAGTGT AGTGCCCTCA 
ACTTGGCGGG ATTTAGACAA TCGAGAGGAC CGATTCGTGG GTCGTCACCG AATCCTGGAC 
AATCTATTTA CCGCCGTGCG TTTGGCAAGA CCCGCCGACG TATCCGAGCT GACTTTGGAT 
CCTGCGAGCG CCCAACGACG TAATCCCAAA AAGAAGACTA CCATTAAGCA GTTGTTCTCG 
GTAACCACCA AGTTTCAAAA GACACTGAGA CGCGGCATTT ACTTCTCTTG TATTATTCAT 
TGCGATGATA AAGTGCTGGT CACCAGCGAA GACTTTCCAC CGGTCATCGA GGTGGATGAA 
TCCTATCCGA GTGCTTTGCA CATGGATTAC TACTGGCTCA TGAAAGTTGC CTGCACCTGG 
GAGGATGTTA AGTCTCTACG TTCGGATATG GAGCGCAATC TTACCTCCGC TGTTCACTTC 
CGCACTAAGC TTCTATCGGC GGTCTGTCAG ATGCAATCGG CACTAGGCAT TACGGACTTG 
GGCCAACTTT ACTATAAACC ATTGCGGGAT GCTCAGGGCA CTGTGGTCCT AACCTGTGTG 
CAGTCGGTAA AGAGCCAAAA GGCCGTCTCC ATCTTAAACT CGAGATGGGT TCCAGTCAGC 
AAGCTGCAAA AGAAGCTAGG TGCTCTACAC GAGGACTACA CCATTAACGA GCTGCTCATC 
TCCTCGATTG GAGATCAATT GCACTATCAA CAGGCGGCGC TGCAGCGTTT GGAGCCCGGT 
CTTTACCTGG GCTACCTAAA GATGCAATGC TCCATAGACC AGATCCAGGT GGTTGTGCCC 
GTGAAAACGC CGAATGTCTT GCCCCACTGC AAGGTGCGCG AAAACAGTCA CATCACGGCC 
GAAGAGTGGC AGGTGCTCCA CCGTTACAGT AGCGATCCTC TACGCCTGCC GTTGGACTTT 
AGCGCTCAAG GAGGAGATGG AGCCTCCGGA GCAACAGCTA CTACGGAGGT GCAACGCCTA 
TTTTTATACG ATCTCACCAA TGCAATGCAC AAACTGTTTG CCAGTATGAA CATCAAAATC 
GCTGATGCAA CCACTCACCG CTTGTACGAC GTGGAGGTGA TTGAGCATAG TCCGGATATT 
AGTTTCCTCG TGGTGTGTCC ATCCGCCGAG TCCTCATGCG CTGTGCCCGG TCAGTCTGAG 
TTACTGCTAC AGCGAGATGA CTTGGCCAGC TTGAGTATCC AGGCCTTTGA GATGATTCAC 
CTGCGCACTT ATCAGCCGGC CATCATTCAG AAGTACGCCC GTTTATCTTG CATCCTTGAA 
TTGGATACCG CACTGGCCAC ACATTCGCTT CGCGAGGCAT TCTCCAGCAG TGAACTGCAG 
GCGGCCAAGG AACGTTTGGC CACGCTGCAG GAGCTCAGTG CGAGTCTTAC GATTGTGTGG 
AAAAGTGTGC GATGGCTAAT GGATGTGGTG GCATATGCGC GAAACAAAAA CGCTCAACCC 
TCGTTGGCTA TGCGAGAAAT TCTGGACTTT GCACAGCAAA GACAGGACGA AGCGGTAGCG 
ACATCGGCAG GAGGTTCCGC GAACAAACAG CTGCTGCAGC TGCCCATCCG CGAGAGCAAG 
TTCTCCAAGA CGGGCCAGGG AAGAGGCAGT TGGCCGGGAC CGGGAACTAG TGAAGATCAA 
TCGCAGAACA AGCCGGAGCA CTCCAAGTCT GAACAGAATC TGGAACTGAG TGCTATCACG 
CCAGTGGAAC CTGCATCCAA TCAAGTTCCT ACACAGCAGC AGCAGCCGCA GCAGCAATCG 
CAGCAACAGC AACTCTTACA AGTTTCCAAC ATCAGCGAAT ACGCGGGATC GATTTGCTCA 
GAAGTATCAT TTAGGAAGAA TAGTGGCGAT TCCATGAGCT CCACCTACAC GTCCCGGAGC 
TTCTACTCTG CCGTGGACTC AGCCTCAGAT GGAAATAGCA CAAACAGTGT GTTTGCCATT 
CCGCCCTCTC GTTCCGACGA CACTCTGGCG GATGCACTGC GCCACTCGCA AGCGGTAGCT 
GCGCAACGCA AACGAACCAG CTCTAATATC GCATCTCATA CCAATCCCTT GATTACGGTG 
CACAGCTCTA GTTCGGCTCC GTACCTGGCT GGATCCAGTG TTTCCTTGAG AAGCGGCAAC 
GGAGCCTTCG CAACGGATTT GGAGCACAAA CCGCTGAAGC CAGCGACAAA GGCTGCATCT 
AGTGCAAACC TGCGCGAAGG TGGACCCTAT TTGAAAAGCA CGTTAGCTGA ACTACGAGCT 
GTTGGAGGTG AGGAGCCGGT GGCCAGTACT TCAAAGGCCT CTTCGATGAA GAGCTTGTCG 
CGGCAGAGCA GCGAAGAGGA TTCCGCCAGC TGTTCCAGTC TCAACGCAGA GCAGACATCG 
GGGATCATTC AAGTCTATAC CGCCTACAGT ACGGGTCTGG CCAGTGGAAC CAGCTTAAAG 
ATTCACGTAA CTCCGAAGAC GACTGCCCGC GAAGTGATCA ACCTGGTGGT GAAGCAACTT 
AACATGGCCG TGGTTCTCAA AGGGAACAAT GGCCCCATCT ATGGACCCGA GATGCTAGAG 
AACTTTTGCC TGGTTGCGGT GATTGGAGCA AGGGAACGCT GCCTGCGAGA TGACTTCAAA 
CCGCTGCAAC TGCAGAATCC CTGGAAGAAG GGCCGTCTGT ACGTCCGCAA GAAGCATGAG 
CTGCTGGCGG CCATCGAACA CTCGAATCGG AAATCGCATT TGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wake ( NM_001202337.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189266.1 Drosophila melanogaster wide awake (wake), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05720
Clone ID Related Accession (Same CDS sequence)	NM_142819.3 , NM_001202337.2
Accession Version	NM_001202337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wide awake, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_001202337.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATTCC TACTGCCCCA TTCGCGAACC GATTCCCAAT CGAATTCCCA ATCGCAACTG 
TGCATTTCGC GGCACTCGCA GCCGAAAAAT GCCAAGCTGC GGGATGATCA CGATGAGACG 
GGCTCATCGG AATTGGATTC ACCACCGCTG GGTGATGAGA ATGCCTCGGC GAATGGCCAA 
CTTTCGGTGC ACAGGCAGCC GGTACAGCTG CGGAGTAAGA TCCCGGGAAA CACACCTCAT 
CCGCGGGCCA GCAAAATGTC CAAGAAACAG CTGAAGTTGG CCCAGGCCCA GCTGGACAAA 
CTGACGCAGA ACAATCTGCA TCTGCATGCT CTCTTCTCGG CAGTGGAGCA TGGTCACCTG 
GAGAAGGCGC GCACTATTCT AGAGTCCACC GACGTAGATG TAAACAGCAT CAATAACGAT 
GGACTATCTG CTCTGGACTT GGCGGTGTTA AGCAATAATC GCTCCATGAC CAGGATGCTG 
CTTCAGCATG GCGCTGTGGA GGGCTCTCAG TTCTCTGTGG ACACCATTGG CACCAAACTG 
AACGGCTTGC TCAAGGACGC CGAGTCAAGA ATTCACGATC TAAGTGGACC GGAGGGCCTC 
TGTCCGCCGG TGTTTGCATC GCGTCCCTCC ATCTCGAGCA TCATAATTGG CAATTCCAGT 
GCATCGGTAA CTGGCTGCAC GGGCAGCGAG GTGGAAAAAC AGATAGGCAT CTGGGAGCGC 
CGAGTTAAGG GACTGCGTCG CTTGCAGCTC GGTTGGGATC AGGCCAGGCC GCCAGATGCA 
CCCGCCTCCG TGGTCGTCGA CGTCACTGGC GACAATTCCA TCAGCGTACA AATCCTAGAG 
CCCTTCGAGG GGGCCATCGG CACGAAATTT AAAGTACAAT GGTCGACCCG CGCGGATTTC 
AACAACGTTG TGGGCGAGAG TGAGCTACTG GAATGGATCA GCTTCCATGG CACGATGGGT 
GCCCAGTGTC ACATATCGGG CCTCACCCAG GGTCGTCGCT ACTTCCTGCG AGCCGCGTGC 
GGCAATGTGA AGGGATGGGG CACCTATCGA ACCTCTGTGC CGGCTAGTGT AGTGCCCTCA 
ACTTGGCGGG ATTTAGACAA TCGAGAGGAC CGATTCGTGG GTCGTCACCG AATCCTGGAC 
AATCTATTTA CCGCCGTGCG TTTGGCAAGA CCCGCCGACG TATCCGAGCT GACTTTGGAT 
CCTGCGAGCG CCCAACGACG TAATCCCAAA AAGAAGACTA CCATTAAGCA GTTGTTCTCG 
GTAACCACCA AGTTTCAAAA GACACTGAGA CGCGGCATTT ACTTCTCTTG TATTATTCAT 
TGCGATGATA AAGTGCTGGT CACCAGCGAA GACTTTCCAC CGGTCATCGA GGTGGATGAA 
TCCTATCCGA GTGCTTTGCA CATGGATTAC TACTGGCTCA TGAAAGTTGC CTGCACCTGG 
GAGGATGTTA AGTCTCTACG TTCGGATATG GAGCGCAATC TTACCTCCGC TGTTCACTTC 
CGCACTAAGC TTCTATCGGC GGTCTGTCAG ATGCAATCGG CACTAGGCAT TACGGACTTG 
GGCCAACTTT ACTATAAACC ATTGCGGGAT GCTCAGGGCA CTGTGGTCCT AACCTGTGTG 
CAGTCGGTAA AGAGCCAAAA GGCCGTCTCC ATCTTAAACT CGAGATGGGT TCCAGTCAGC 
AAGCTGCAAA AGAAGCTAGG TGCTCTACAC GAGGACTACA CCATTAACGA GCTGCTCATC 
TCCTCGATTG GAGATCAATT GCACTATCAA CAGGCGGCGC TGCAGCGTTT GGAGCCCGGT 
CTTTACCTGG GCTACCTAAA GATGCAATGC TCCATAGACC AGATCCAGGT GGTTGTGCCC 
GTGAAAACGC CGAATGTCTT GCCCCACTGC AAGGTGCGCG AAAACAGTCA CATCACGGCC 
GAAGAGTGGC AGGTGCTCCA CCGTTACAGT AGCGATCCTC TACGCCTGCC GTTGGACTTT 
AGCGCTCAAG GAGGAGATGG AGCCTCCGGA GCAACAGCTA CTACGGAGGT GCAACGCCTA 
TTTTTATACG ATCTCACCAA TGCAATGCAC AAACTGTTTG CCAGTATGAA CATCAAAATC 
GCTGATGCAA CCACTCACCG CTTGTACGAC GTGGAGGTGA TTGAGCATAG TCCGGATATT 
AGTTTCCTCG TGGTGTGTCC ATCCGCCGAG TCCTCATGCG CTGTGCCCGG TCAGTCTGAG 
TTACTGCTAC AGCGAGATGA CTTGGCCAGC TTGAGTATCC AGGCCTTTGA GATGATTCAC 
CTGCGCACTT ATCAGCCGGC CATCATTCAG AAGTACGCCC GTTTATCTTG CATCCTTGAA 
TTGGATACCG CACTGGCCAC ACATTCGCTT CGCGAGGCAT TCTCCAGCAG TGAACTGCAG 
GCGGCCAAGG AACGTTTGGC CACGCTGCAG GAGCTCAGTG CGAGTCTTAC GATTGTGTGG 
AAAAGTGTGC GATGGCTAAT GGATGTGGTG GCATATGCGC GAAACAAAAA CGCTCAACCC 
TCGTTGGCTA TGCGAGAAAT TCTGGACTTT GCACAGCAAA GACAGGACGA AGCGGTAGCG 
ACATCGGCAG GAGGTTCCGC GAACAAACAG CTGCTGCAGC TGCCCATCCG CGAGAGCAAG 
TTCTCCAAGA CGGGCCAGGG AAGAGGCAGT TGGCCGGGAC CGGGAACTAG TGAAGATCAA 
TCGCAGAACA AGCCGGAGCA CTCCAAGTCT GAACAGAATC TGGAACTGAG TGCTATCACG 
CCAGTGGAAC CTGCATCCAA TCAAGTTCCT ACACAGCAGC AGCAGCCGCA GCAGCAATCG 
CAGCAACAGC AACTCTTACA AGTTTCCAAC ATCAGCGAAT ACGCGGGATC GATTTGCTCA 
GAAGTATCAT TTAGGAAGAA TAGTGGCGAT TCCATGAGCT CCACCTACAC GTCCCGGAGC 
TTCTACTCTG CCGTGGACTC AGCCTCAGAT GGAAATAGCA CAAACAGTGT GTTTGCCATT 
CCGCCCTCTC GTTCCGACGA CACTCTGGCG GATGCACTGC GCCACTCGCA AGCGGTAGCT 
GCGCAACGCA AACGAACCAG CTCTAATATC GCATCTCATA CCAATCCCTT GATTACGGTG 
CACAGCTCTA GTTCGGCTCC GTACCTGGCT GGATCCAGTG TTTCCTTGAG AAGCGGCAAC 
GGAGCCTTCG CAACGGATTT GGAGCACAAA CCGCTGAAGC CAGCGACAAA GGCTGCATCT 
AGTGCAAACC TGCGCGAAGG TGGACCCTAT TTGAAAAGCA CGTTAGCTGA ACTACGAGCT 
GTTGGAGGTG AGGAGCCGGT GGCCAGTACT TCAAAGGCCT CTTCGATGAA GAGCTTGTCG 
CGGCAGAGCA GCGAAGAGGA TTCCGCCAGC TGTTCCAGTC TCAACGCAGA GCAGACATCG 
GGGATCATTC AAGTCTATAC CGCCTACAGT ACGGGTCTGG CCAGTGGAAC CAGCTTAAAG 
ATTCACGTAA CTCCGAAGAC GACTGCCCGC GAAGTGATCA ACCTGGTGGT GAAGCAACTT 
AACATGGCCG TGGTTCTCAA AGGGAACAAT GGCCCCATCT ATGGACCCGA GATGCTAGAG 
AACTTTTGCC TGGTTGCGGT GATTGGAGCA AGGGAACGCT GCCTGCGAGA TGACTTCAAA 
CCGCTGCAAC TGCAGAATCC CTGGAAGAAG GGCCGTCTGT ACGTCCGCAA GAAGCATGAG 
CTGCTGGCGG CCATCGAACA CTCGAATCGG AAATCGCATT TGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189266.1
CDS	569..4336
Translation	MEFLLPHSRTDSQSNSQSQLCISRHSQPKNAKLRDDHDETGSSELDSPPLGDENASANGQLSVHRQPVQLRSKIPGNTPHPRASKMSKKQLKLAQAQLDKLTQNNLHLHALFSAVEHGHLEKARTILESTDVDVNSINNDGLSALDLAVLSNNRSMTRMLLQHGAVEGSQFSVDTIGTKLNGLLKDAESRIHDLSGPEGLCPPVFASRPSISSIIIGNSSASVTGCTGSEVEKQIGIWERRVKGLRRLQLGWDQARPPDAPASVVVDVTGDNSISVQILEPFEGAIGTKFKVQWSTRADFNNVVGESELLEWISFHGTMGAQCHISGLTQGRRYFLRAACGNVKGWGTYRTSVPASVVPSTWRDLDNREDRFVGRHRILDNLFTAVRLARPADVSELTLDPASAQRRNPKKKTTIKQLFSVTTKFQKTLRRGIYFSCIIHCDDKVLVTSEDFPPVIEVDESYPSALHMDYYWLMKVACTWEDVKSLRSDMERNLTSAVHFRTKLLSAVCQMQSALGITDLGQLYYKPLRDAQGTVVLTCVQSVKSQKAVSILNSRWVPVSKLQKKLGALHEDYTINELLISSIGDQLHYQQAALQRLEPGLYLGYLKMQCSIDQIQVVVPVKTPNVLPHCKVRENSHITAEEWQVLHRYSSDPLRLPLDFSAQGGDGASGATATTEVQRLFLYDLTNAMHKLFASMNIKIADATTHRLYDVEVIEHSPDISFLVVCPSAESSCAVPGQSELLLQRDDLASLSIQAFEMIHLRTYQPAIIQKYARLSCILELDTALATHSLREAFSSSELQAAKERLATLQELSASLTIVWKSVRWLMDVVAYARNKNAQPSLAMREILDFAQQRQDEAVATSAGGSANKQLLQLPIRESKFSKTGQGRGSWPGPGTSEDQSQNKPEHSKSEQNLELSAITPVEPASNQVPTQQQQPQQQSQQQQLLQVSNISEYAGSICSEVSFRKNSGDSMSSTYTSRSFYSAVDSASDGNSTNSVFAIPPSRSDDTLADALRHSQAVAAQRKRTSSNIASHTNPLITVHSSSSAPYLAGSSVSLRSGNGAFATDLEHKPLKPATKAASSANLREGGPYLKSTLAELRAVGGEEPVASTSKASSMKSLSRQSSEEDSASCSSLNAEQTSGIIQVYTAYSTGLASGTSLKIHVTPKTTAREVINLVVKQLNMAVVLKGNNGPIYGPEMLENFCLVAVIGARERCLRDDFKPLQLQNPWKKGRLYVRKKHELLAAIEHSNRKSHLI

Target ORF information:

RefSeq Version	NM_001202337.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wide awake (wake), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202337.2

ORF Insert Sequence:

ATGGAATTCC TACTGCCCCA TTCGCGAACC GATTCCCAAT CGAATTCCCA ATCGCAACTG 
TGCATTTCGC GGCACTCGCA GCCGAAAAAT GCCAAGCTGC GGGATGATCA CGATGAGACG 
GGCTCATCGG AATTGGATTC ACCACCGCTG GGTGATGAGA ATGCCTCGGC GAATGGCCAA 
CTTTCGGTGC ACAGGCAGCC GGTACAGCTG CGGAGTAAGA TCCCGGGAAA CACACCTCAT 
CCGCGGGCCA GCAAAATGTC CAAGAAACAG CTGAAGTTGG CCCAGGCCCA GCTGGACAAA 
CTGACGCAGA ACAATCTGCA TCTGCATGCT CTCTTCTCGG CAGTGGAGCA TGGTCACCTG 
GAGAAGGCGC GCACTATTCT AGAGTCCACC GACGTAGATG TAAACAGCAT CAATAACGAT 
GGACTATCTG CTCTGGACTT GGCGGTGTTA AGCAATAATC GCTCCATGAC CAGGATGCTG 
CTTCAGCATG GCGCTGTGGA GGGCTCTCAG TTCTCTGTGG ACACCATTGG CACCAAACTG 
AACGGCTTGC TCAAGGACGC CGAGTCAAGA ATTCACGATC TAAGTGGACC GGAGGGCCTC 
TGTCCGCCGG TGTTTGCATC GCGTCCCTCC ATCTCGAGCA TCATAATTGG CAATTCCAGT 
GCATCGGTAA CTGGCTGCAC GGGCAGCGAG GTGGAAAAAC AGATAGGCAT CTGGGAGCGC 
CGAGTTAAGG GACTGCGTCG CTTGCAGCTC GGTTGGGATC AGGCCAGGCC GCCAGATGCA 
CCCGCCTCCG TGGTCGTCGA CGTCACTGGC GACAATTCCA TCAGCGTACA AATCCTAGAG 
CCCTTCGAGG GGGCCATCGG CACGAAATTT AAAGTACAAT GGTCGACCCG CGCGGATTTC 
AACAACGTTG TGGGCGAGAG TGAGCTACTG GAATGGATCA GCTTCCATGG CACGATGGGT 
GCCCAGTGTC ACATATCGGG CCTCACCCAG GGTCGTCGCT ACTTCCTGCG AGCCGCGTGC 
GGCAATGTGA AGGGATGGGG CACCTATCGA ACCTCTGTGC CGGCTAGTGT AGTGCCCTCA 
ACTTGGCGGG ATTTAGACAA TCGAGAGGAC CGATTCGTGG GTCGTCACCG AATCCTGGAC 
AATCTATTTA CCGCCGTGCG TTTGGCAAGA CCCGCCGACG TATCCGAGCT GACTTTGGAT 
CCTGCGAGCG CCCAACGACG TAATCCCAAA AAGAAGACTA CCATTAAGCA GTTGTTCTCG 
GTAACCACCA AGTTTCAAAA GACACTGAGA CGCGGCATTT ACTTCTCTTG TATTATTCAT 
TGCGATGATA AAGTGCTGGT CACCAGCGAA GACTTTCCAC CGGTCATCGA GGTGGATGAA 
TCCTATCCGA GTGCTTTGCA CATGGATTAC TACTGGCTCA TGAAAGTTGC CTGCACCTGG 
GAGGATGTTA AGTCTCTACG TTCGGATATG GAGCGCAATC TTACCTCCGC TGTTCACTTC 
CGCACTAAGC TTCTATCGGC GGTCTGTCAG ATGCAATCGG CACTAGGCAT TACGGACTTG 
GGCCAACTTT ACTATAAACC ATTGCGGGAT GCTCAGGGCA CTGTGGTCCT AACCTGTGTG 
CAGTCGGTAA AGAGCCAAAA GGCCGTCTCC ATCTTAAACT CGAGATGGGT TCCAGTCAGC 
AAGCTGCAAA AGAAGCTAGG TGCTCTACAC GAGGACTACA CCATTAACGA GCTGCTCATC 
TCCTCGATTG GAGATCAATT GCACTATCAA CAGGCGGCGC TGCAGCGTTT GGAGCCCGGT 
CTTTACCTGG GCTACCTAAA GATGCAATGC TCCATAGACC AGATCCAGGT GGTTGTGCCC 
GTGAAAACGC CGAATGTCTT GCCCCACTGC AAGGTGCGCG AAAACAGTCA CATCACGGCC 
GAAGAGTGGC AGGTGCTCCA CCGTTACAGT AGCGATCCTC TACGCCTGCC GTTGGACTTT 
AGCGCTCAAG GAGGAGATGG AGCCTCCGGA GCAACAGCTA CTACGGAGGT GCAACGCCTA 
TTTTTATACG ATCTCACCAA TGCAATGCAC AAACTGTTTG CCAGTATGAA CATCAAAATC 
GCTGATGCAA CCACTCACCG CTTGTACGAC GTGGAGGTGA TTGAGCATAG TCCGGATATT 
AGTTTCCTCG TGGTGTGTCC ATCCGCCGAG TCCTCATGCG CTGTGCCCGG TCAGTCTGAG 
TTACTGCTAC AGCGAGATGA CTTGGCCAGC TTGAGTATCC AGGCCTTTGA GATGATTCAC 
CTGCGCACTT ATCAGCCGGC CATCATTCAG AAGTACGCCC GTTTATCTTG CATCCTTGAA 
TTGGATACCG CACTGGCCAC ACATTCGCTT CGCGAGGCAT TCTCCAGCAG TGAACTGCAG 
GCGGCCAAGG AACGTTTGGC CACGCTGCAG GAGCTCAGTG CGAGTCTTAC GATTGTGTGG 
AAAAGTGTGC GATGGCTAAT GGATGTGGTG GCATATGCGC GAAACAAAAA CGCTCAACCC 
TCGTTGGCTA TGCGAGAAAT TCTGGACTTT GCACAGCAAA GACAGGACGA AGCGGTAGCG 
ACATCGGCAG GAGGTTCCGC GAACAAACAG CTGCTGCAGC TGCCCATCCG CGAGAGCAAG 
TTCTCCAAGA CGGGCCAGGG AAGAGGCAGT TGGCCGGGAC CGGGAACTAG TGAAGATCAA 
TCGCAGAACA AGCCGGAGCA CTCCAAGTCT GAACAGAATC TGGAACTGAG TGCTATCACG 
CCAGTGGAAC CTGCATCCAA TCAAGTTCCT ACACAGCAGC AGCAGCCGCA GCAGCAATCG 
CAGCAACAGC AACTCTTACA AGTTTCCAAC ATCAGCGAAT ACGCGGGATC GATTTGCTCA 
GAAGTATCAT TTAGGAAGAA TAGTGGCGAT TCCATGAGCT CCACCTACAC GTCCCGGAGC 
TTCTACTCTG CCGTGGACTC AGCCTCAGAT GGAAATAGCA CAAACAGTGT GTTTGCCATT 
CCGCCCTCTC GTTCCGACGA CACTCTGGCG GATGCACTGC GCCACTCGCA AGCGGTAGCT 
GCGCAACGCA AACGAACCAG CTCTAATATC GCATCTCATA CCAATCCCTT GATTACGGTG 
CACAGCTCTA GTTCGGCTCC GTACCTGGCT GGATCCAGTG TTTCCTTGAG AAGCGGCAAC 
GGAGCCTTCG CAACGGATTT GGAGCACAAA CCGCTGAAGC CAGCGACAAA GGCTGCATCT 
AGTGCAAACC TGCGCGAAGG TGGACCCTAT TTGAAAAGCA CGTTAGCTGA ACTACGAGCT 
GTTGGAGGTG AGGAGCCGGT GGCCAGTACT TCAAAGGCCT CTTCGATGAA GAGCTTGTCG 
CGGCAGAGCA GCGAAGAGGA TTCCGCCAGC TGTTCCAGTC TCAACGCAGA GCAGACATCG 
GGGATCATTC AAGTCTATAC CGCCTACAGT ACGGGTCTGG CCAGTGGAAC CAGCTTAAAG 
ATTCACGTAA CTCCGAAGAC GACTGCCCGC GAAGTGATCA ACCTGGTGGT GAAGCAACTT 
AACATGGCCG TGGTTCTCAA AGGGAACAAT GGCCCCATCT ATGGACCCGA GATGCTAGAG 
AACTTTTGCC TGGTTGCGGT GATTGGAGCA AGGGAACGCT GCCTGCGAGA TGACTTCAAA 
CCGCTGCAAC TGCAGAATCC CTGGAAGAAG GGCCGTCTGT ACGTCCGCAA GAAGCATGAG 
CTGCTGGCGG CCATCGAACA CTCGAATCGG AAATCGCATT TGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wake ( NM_001260316.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247245.1 Drosophila melanogaster wide awake (wake), transcript variant E, mRNA.

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CloneID	OFa05719
Clone ID Related Accession (Same CDS sequence)	NM_001260316.1 , NM_001260315.1
Accession Version	NM_001260316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wide awake, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACAGAT GCTACCACAG CGATAGCACT AAACTTCCCA ATATGCCGCA AAAGCTCCCG 
ATGAGCAGCT GTCTGCGTTC TCCGGCGGTC TTCGTTCGCC AGAAATCGTT TCACACGCTC 
AACGAGCTGA CCAGGTTCTG TCAATTGAAA GTGTCCCCAC ATCGGTCCAC CGCCACAGCC 
ACCTCCACAT CATCGTCACC ATCATCGTCG CCGGCAGCGG CGCAACAGCA GCGTCGCCAC 
AGTCACTATG CCATCGATGA CAACATATTG CCGCAAGATA AACGTGACGG TGACATCGAT 
AGTGTCAGGC TGCGGGAGAA GGTGGTTAAT CCCAGTGGCG GCAATAACAA TAACCAGGAC 
AACTATCCCA CCTATTATCC GGCACCATTG CTGTCCACCA GTCGCTGGTC GGAGCAGTTG 
ACACAGCATG CGGCAGTAGC CAAGATACGG ACAGCCATCT CCTGCCACTC CCAGGATCTC 
AGGGAAATGG AATTCCTACT GCCCCATTCG CGAACCGATT CCCAATCGAA TTCCCAATCG 
CAACTGTGCA TTTCGCGGCA CTCGCAGCCG AAAAATGCCA AGCTGCGGGA TGATCACGAT 
GAGACGGGCT CATCGGAATT GGATTCACCA CCGCTGGGTG ATGAGAATGC CTCGGCGAAT 
GGCCAACTTT CGGTGCACAG GCAGCCGGTA CAGCTGCGGA GTAAGATCCC GGGAAACACA 
CCTCATCCGC GGGCCAGCAA AATGTCCAAG AAACAGCTGA AGTTGGCCCA GGCCCAGCTG 
GACAAACTGA CGCAGAACAA TCTGCATCTG CATGCTCTCT TCTCGGCAGT GGAGCATGGT 
CACCTGGAGA AGGCGCGCAC TATTCTAGAG TCCACCGACG TAGATGTAAA CAGCATCAAT 
AACGATGGAC TATCTGCTCT GGACTTGGCG GTGTTAAGCA ATAATCGCTC CATGACCAGG 
ATGCTGCTTC AGCATGGCGC TGTGGAGGGC TCTCAGTTCT CTGTGGACAC CATTGGCACC 
AAACTGAACG GCTTGCTCAA GGACGCCGAG TCAAGAATTC ACGATCTAAG TGGACCGGAG 
GGCCTCTGTC CGCCGGTGTT TGCATCGCGT CCCTCCATCT CGAGCATCAT AATTGGCAAT 
TCCAGTGCAT CGGTAACTGG CTGCACGGGC AGCGAGGTGG AAAAACAGAT AGGCATCTGG 
GAGCGCCGAG TTAAGGGACT GCGTCGCTTG CAGCTCGGTT GGGATCAGGC CAGGCCGCCA 
GATGCACCCG CCTCCGTGGT CGTCGACGTC ACTGGCGACA ATTCCATCAG CGTACAAATC 
CTAGAGCCCT TCGAGGGGGC CATCGGCACG AAATTTAAAG TACAATGGTC GACCCGCGCG 
GATTTCAACA ACGTTGTGGG CGAGAGTGAG CTACTGGAAT GGATCAGCTT CCATGGCACG 
ATGGGTGCCC AGTGTCACAT ATCGGGCCTC ACCCAGGGTC GTCGCTACTT CCTGCGAGCC 
GCGTGCGGCA ATGTGAAGGG ATGGGGCACC TATCGAACCT CTGTGCCGGC TAGTGTAGTG 
CCCTCAACTT GGCGGGATTT AGACAATCGA GAGGACCGAT TCGTGGGTCG TCACCGAATC 
CTGGACAATC TATTTACCGC CGTGCGTTTG GCAAGACCCG CCGACGTATC CGAGCTGACT 
TTGGATCCTG CGAGCGCCCA ACGACGTAAT CCCAAAAAGA AGACTACCAT TAAGCAGTTG 
TTCTCGGTAA CCACCAAGTT TCAAAAGACA CTGAGACGCG GCATTTACTT CTCTTGTATT 
ATTCATTGCG ATGATAAAGT GCTGGTCACC AGCGAAGACT TTCCACCGGT CATCGAGGTG 
GATGAATCCT ATCCGAGTGC TTTGCACATG GATTACTACT GGCTCATGAA AGTTGCCTGC 
ACCTGGGAGG ATGTTAAGTC TCTACGTTCG GATATGGAGC GCAATCTTAC CTCCGCTGTT 
CACTTCCGCA CTAAGCTTCT ATCGGCGGTC TGTCAGATGC AATCGGCACT AGGCATTACG 
GACTTGGGCC AACTTTACTA TAAACCATTG CGGGATGCTC AGGGCACTGT GGTCCTAACC 
TGTGTGCAGT CGGTAAAGAG CCAAAAGGCC GTCTCCATCT TAAACTCGAG ATGGGTTCCA 
GTCAGCAAGC TGCAAAAGAA GCTAGGTGCT CTACACGAGG ACTACACCAT TAACGAGCTG 
CTCATCTCCT CGATTGGAGA TCAATTGCAC TATCAACAGG CGGCGCTGCA GCGTTTGGAG 
CCCGGTCTTT ACCTGGGCTA CCTAAAGATG CAATGCTCCA TAGACCAGAT CCAGGTGGTT 
GTGCCCGTGA AAACGCCGAA TGTCTTGCCC CACTGCAAGG TGCGCGAAAA CAGTCACATC 
ACGGCCGAAG AGTGGCAGGT GCTCCACCGT TACAGTAGCG ATCCTCTACG CCTGCCGTTG 
GACTTTAGCG CTCAAGGAGG AGATGGAGCC TCCGGAGCAA CAGCTACTAC GGAGGTGCAA 
CGCCTATTTT TATACGATCT CACCAATGCA ATGCACAAAC TGTTTGCCAG TATGAACATC 
AAAATCGCTG ATGCAACCAC TCACCGCTTG TACGACGTGG AGGTGATTGA GCATAGTCCG 
GATATTAGTT TCCTCGTGGT GTGTCCATCC GCCGAGTCCT CATGCGCTGT GCCCGGTCAG 
TCTGAGTTAC TGCTACAGCG AGATGACTTG GCCAGCTTGA GTATCCAGGC CTTTGAGATG 
ATTCACCTGC GCACTTATCA GCCGGCCATC ATTCAGAAGT ACGCCCGTTT ATCTTGCATC 
CTTGAATTGG ATACCGCACT GGCCACACAT TCGCTTCGCG AGGCATTCTC CAGCAGTGAA 
CTGCAGGCGG CCAAGGAACG TTTGGCCACG CTGCAGGAGC TCAGTGCGAG TCTTACGATT 
GTGTGGAAAA GTGTGCGATG GCTAATGGAT GTGGTGGCAT ATGCGCGAAA CAAAAACGCT 
CAACCCTCGT TGGCTATGCG AGAAATTCTG GACTTTGCAC AGCAAAGACA GGACGAAGCG 
GTAGCGACAT CGGCAGGAGG TTCCGCGAAC AAACAGCTGC TGCAGCTGCC CATCCGCGAG 
AGCAAGTTCT CCAAGACGGG CCAGGGAAGA GGCAGTTGGC CGGGACCGGG AACTAGTGAA 
GATCAATCGC AGAACAAGCC GGAGCACTCC AAGTCTGAAC AGAATCTGGA ACTGAGTGCT 
ATCACGCCAG TGGAACCTGC ATCCAATCAA GTTCCTACAC AGCAGCAGCA GCCGCAGCAG 
CAATCGCAGC AACAGCAACT CTTACAAGTT TCCAACATCA GCGAATACGC GGGATCGATT 
TGCTCAGAAG TATCATTTAG GAAGAATAGT GGCGATTCCA TGAGCTCCAC CTACACGTCC 
CGGAGCTTCT ACTCTGCCGT GGACTCAGCC TCAGATGGAA ATAGCACAAA CAGTGTGTTT 
GCCATTCCGC CCTCTCGTTC CGACGACACT CTGGCGGATG CACTGCGCCA CTCGCAAGCG 
GTAGCTGCGC AACGCAAACG AACCAGCTCT AATATCGCAT CTCATACCAA TCCCTTGATT 
ACGGTGCACA GCTCTAGTTC GGCTCCGTAC CTGGCTGGAT CCAGTGTTTC CTTGAGAAGC 
GGCAACGGAG CCTTCGCAAC GGATTTGGAG CACAAACCGC TGAAGCCAGC GACAAAGGCT 
GCATCTAGTG CAAACCTGCG CGAAGGTGGA CCCTATTTGA AAAGCACGTT AGCTGAACTA 
CGAGCTGTTG GAGGTGAGGA GCCGGTGGCC AGTACTTCAA AGGCCTCTTC GATGAAGAGC 
TTGTCGCGGC AGAGCAGCGA AGAGGATTCC GCCAGCTGTT CCAGTCTCAA CGCAGAGCAG 
ACATCGGGGA TCATTCAAGT CTATACCGCC TACAGTACGG GTCTGGCCAG TGGAACCAGC 
TTAAAGATTC ACGTAACTCC GAAGACGACT GCCCGCGAAG TGATCAACCT GGTGGTGAAG 
CAACTTAACA TGGCCGTGGT TCTCAAAGGG AACAATGGCC CCATCTATGG ACCCGAGATG 
CTAGAGAACT TTTGCCTGGT TGCGGTGATT GGAGCAAGGG AACGCTGCCT GCGAGATGAC 
TTCAAACCGC TGCAACTGCA GAATCCCTGG AAGAAGGGCC GTCTGTACGT CCGCAAGAAG 
CATGAGCTGC TGGCGGCCAT CGAACACTCG AATCGGAAAT CGCATTTGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247245.1
CDS	374..4627
Translation	MNRCYHSDSTKLPNMPQKLPMSSCLRSPAVFVRQKSFHTLNELTRFCQLKVSPHRSTATATSTSSSPSSSPAAAQQQRRHSHYAIDDNILPQDKRDGDIDSVRLREKVVNPSGGNNNNQDNYPTYYPAPLLSTSRWSEQLTQHAAVAKIRTAISCHSQDLREMEFLLPHSRTDSQSNSQSQLCISRHSQPKNAKLRDDHDETGSSELDSPPLGDENASANGQLSVHRQPVQLRSKIPGNTPHPRASKMSKKQLKLAQAQLDKLTQNNLHLHALFSAVEHGHLEKARTILESTDVDVNSINNDGLSALDLAVLSNNRSMTRMLLQHGAVEGSQFSVDTIGTKLNGLLKDAESRIHDLSGPEGLCPPVFASRPSISSIIIGNSSASVTGCTGSEVEKQIGIWERRVKGLRRLQLGWDQARPPDAPASVVVDVTGDNSISVQILEPFEGAIGTKFKVQWSTRADFNNVVGESELLEWISFHGTMGAQCHISGLTQGRRYFLRAACGNVKGWGTYRTSVPASVVPSTWRDLDNREDRFVGRHRILDNLFTAVRLARPADVSELTLDPASAQRRNPKKKTTIKQLFSVTTKFQKTLRRGIYFSCIIHCDDKVLVTSEDFPPVIEVDESYPSALHMDYYWLMKVACTWEDVKSLRSDMERNLTSAVHFRTKLLSAVCQMQSALGITDLGQLYYKPLRDAQGTVVLTCVQSVKSQKAVSILNSRWVPVSKLQKKLGALHEDYTINELLISSIGDQLHYQQAALQRLEPGLYLGYLKMQCSIDQIQVVVPVKTPNVLPHCKVRENSHITAEEWQVLHRYSSDPLRLPLDFSAQGGDGASGATATTEVQRLFLYDLTNAMHKLFASMNIKIADATTHRLYDVEVIEHSPDISFLVVCPSAESSCAVPGQSELLLQRDDLASLSIQAFEMIHLRTYQPAIIQKYARLSCILELDTALATHSLREAFSSSELQAAKERLATLQELSASLTIVWKSVRWLMDVVAYARNKNAQPSLAMREILDFAQQRQDEAVATSAGGSANKQLLQLPIRESKFSKTGQGRGSWPGPGTSEDQSQNKPEHSKSEQNLELSAITPVEPASNQVPTQQQQPQQQSQQQQLLQVSNISEYAGSICSEVSFRKNSGDSMSSTYTSRSFYSAVDSASDGNSTNSVFAIPPSRSDDTLADALRHSQAVAAQRKRTSSNIASHTNPLITVHSSSSAPYLAGSSVSLRSGNGAFATDLEHKPLKPATKAASSANLREGGPYLKSTLAELRAVGGEEPVASTSKASSMKSLSRQSSEEDSASCSSLNAEQTSGIIQVYTAYSTGLASGTSLKIHVTPKTTAREVINLVVKQLNMAVVLKGNNGPIYGPEMLENFCLVAVIGARERCLRDDFKPLQLQNPWKKGRLYVRKKHELLAAIEHSNRKSHLI

Target ORF information:

RefSeq Version	NM_001260316.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wide awake (wake), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260316.1

ORF Insert Sequence:

ATGAACAGAT GCTACCACAG CGATAGCACT AAACTTCCCA ATATGCCGCA AAAGCTCCCG 
ATGAGCAGCT GTCTGCGTTC TCCGGCGGTC TTCGTTCGCC AGAAATCGTT TCACACGCTC 
AACGAGCTGA CCAGGTTCTG TCAATTGAAA GTGTCCCCAC ATCGGTCCAC CGCCACAGCC 
ACCTCCACAT CATCGTCACC ATCATCGTCG CCGGCAGCGG CGCAACAGCA GCGTCGCCAC 
AGTCACTATG CCATCGATGA CAACATATTG CCGCAAGATA AACGTGACGG TGACATCGAT 
AGTGTCAGGC TGCGGGAGAA GGTGGTTAAT CCCAGTGGCG GCAATAACAA TAACCAGGAC 
AACTATCCCA CCTATTATCC GGCACCATTG CTGTCCACCA GTCGCTGGTC GGAGCAGTTG 
ACACAGCATG CGGCAGTAGC CAAGATACGG ACAGCCATCT CCTGCCACTC CCAGGATCTC 
AGGGAAATGG AATTCCTACT GCCCCATTCG CGAACCGATT CCCAATCGAA TTCCCAATCG 
CAACTGTGCA TTTCGCGGCA CTCGCAGCCG AAAAATGCCA AGCTGCGGGA TGATCACGAT 
GAGACGGGCT CATCGGAATT GGATTCACCA CCGCTGGGTG ATGAGAATGC CTCGGCGAAT 
GGCCAACTTT CGGTGCACAG GCAGCCGGTA CAGCTGCGGA GTAAGATCCC GGGAAACACA 
CCTCATCCGC GGGCCAGCAA AATGTCCAAG AAACAGCTGA AGTTGGCCCA GGCCCAGCTG 
GACAAACTGA CGCAGAACAA TCTGCATCTG CATGCTCTCT TCTCGGCAGT GGAGCATGGT 
CACCTGGAGA AGGCGCGCAC TATTCTAGAG TCCACCGACG TAGATGTAAA CAGCATCAAT 
AACGATGGAC TATCTGCTCT GGACTTGGCG GTGTTAAGCA ATAATCGCTC CATGACCAGG 
ATGCTGCTTC AGCATGGCGC TGTGGAGGGC TCTCAGTTCT CTGTGGACAC CATTGGCACC 
AAACTGAACG GCTTGCTCAA GGACGCCGAG TCAAGAATTC ACGATCTAAG TGGACCGGAG 
GGCCTCTGTC CGCCGGTGTT TGCATCGCGT CCCTCCATCT CGAGCATCAT AATTGGCAAT 
TCCAGTGCAT CGGTAACTGG CTGCACGGGC AGCGAGGTGG AAAAACAGAT AGGCATCTGG 
GAGCGCCGAG TTAAGGGACT GCGTCGCTTG CAGCTCGGTT GGGATCAGGC CAGGCCGCCA 
GATGCACCCG CCTCCGTGGT CGTCGACGTC ACTGGCGACA ATTCCATCAG CGTACAAATC 
CTAGAGCCCT TCGAGGGGGC CATCGGCACG AAATTTAAAG TACAATGGTC GACCCGCGCG 
GATTTCAACA ACGTTGTGGG CGAGAGTGAG CTACTGGAAT GGATCAGCTT CCATGGCACG 
ATGGGTGCCC AGTGTCACAT ATCGGGCCTC ACCCAGGGTC GTCGCTACTT CCTGCGAGCC 
GCGTGCGGCA ATGTGAAGGG ATGGGGCACC TATCGAACCT CTGTGCCGGC TAGTGTAGTG 
CCCTCAACTT GGCGGGATTT AGACAATCGA GAGGACCGAT TCGTGGGTCG TCACCGAATC 
CTGGACAATC TATTTACCGC CGTGCGTTTG GCAAGACCCG CCGACGTATC CGAGCTGACT 
TTGGATCCTG CGAGCGCCCA ACGACGTAAT CCCAAAAAGA AGACTACCAT TAAGCAGTTG 
TTCTCGGTAA CCACCAAGTT TCAAAAGACA CTGAGACGCG GCATTTACTT CTCTTGTATT 
ATTCATTGCG ATGATAAAGT GCTGGTCACC AGCGAAGACT TTCCACCGGT CATCGAGGTG 
GATGAATCCT ATCCGAGTGC TTTGCACATG GATTACTACT GGCTCATGAA AGTTGCCTGC 
ACCTGGGAGG ATGTTAAGTC TCTACGTTCG GATATGGAGC GCAATCTTAC CTCCGCTGTT 
CACTTCCGCA CTAAGCTTCT ATCGGCGGTC TGTCAGATGC AATCGGCACT AGGCATTACG 
GACTTGGGCC AACTTTACTA TAAACCATTG CGGGATGCTC AGGGCACTGT GGTCCTAACC 
TGTGTGCAGT CGGTAAAGAG CCAAAAGGCC GTCTCCATCT TAAACTCGAG ATGGGTTCCA 
GTCAGCAAGC TGCAAAAGAA GCTAGGTGCT CTACACGAGG ACTACACCAT TAACGAGCTG 
CTCATCTCCT CGATTGGAGA TCAATTGCAC TATCAACAGG CGGCGCTGCA GCGTTTGGAG 
CCCGGTCTTT ACCTGGGCTA CCTAAAGATG CAATGCTCCA TAGACCAGAT CCAGGTGGTT 
GTGCCCGTGA AAACGCCGAA TGTCTTGCCC CACTGCAAGG TGCGCGAAAA CAGTCACATC 
ACGGCCGAAG AGTGGCAGGT GCTCCACCGT TACAGTAGCG ATCCTCTACG CCTGCCGTTG 
GACTTTAGCG CTCAAGGAGG AGATGGAGCC TCCGGAGCAA CAGCTACTAC GGAGGTGCAA 
CGCCTATTTT TATACGATCT CACCAATGCA ATGCACAAAC TGTTTGCCAG TATGAACATC 
AAAATCGCTG ATGCAACCAC TCACCGCTTG TACGACGTGG AGGTGATTGA GCATAGTCCG 
GATATTAGTT TCCTCGTGGT GTGTCCATCC GCCGAGTCCT CATGCGCTGT GCCCGGTCAG 
TCTGAGTTAC TGCTACAGCG AGATGACTTG GCCAGCTTGA GTATCCAGGC CTTTGAGATG 
ATTCACCTGC GCACTTATCA GCCGGCCATC ATTCAGAAGT ACGCCCGTTT ATCTTGCATC 
CTTGAATTGG ATACCGCACT GGCCACACAT TCGCTTCGCG AGGCATTCTC CAGCAGTGAA 
CTGCAGGCGG CCAAGGAACG TTTGGCCACG CTGCAGGAGC TCAGTGCGAG TCTTACGATT 
GTGTGGAAAA GTGTGCGATG GCTAATGGAT GTGGTGGCAT ATGCGCGAAA CAAAAACGCT 
CAACCCTCGT TGGCTATGCG AGAAATTCTG GACTTTGCAC AGCAAAGACA GGACGAAGCG 
GTAGCGACAT CGGCAGGAGG TTCCGCGAAC AAACAGCTGC TGCAGCTGCC CATCCGCGAG 
AGCAAGTTCT CCAAGACGGG CCAGGGAAGA GGCAGTTGGC CGGGACCGGG AACTAGTGAA 
GATCAATCGC AGAACAAGCC GGAGCACTCC AAGTCTGAAC AGAATCTGGA ACTGAGTGCT 
ATCACGCCAG TGGAACCTGC ATCCAATCAA GTTCCTACAC AGCAGCAGCA GCCGCAGCAG 
CAATCGCAGC AACAGCAACT CTTACAAGTT TCCAACATCA GCGAATACGC GGGATCGATT 
TGCTCAGAAG TATCATTTAG GAAGAATAGT GGCGATTCCA TGAGCTCCAC CTACACGTCC 
CGGAGCTTCT ACTCTGCCGT GGACTCAGCC TCAGATGGAA ATAGCACAAA CAGTGTGTTT 
GCCATTCCGC CCTCTCGTTC CGACGACACT CTGGCGGATG CACTGCGCCA CTCGCAAGCG 
GTAGCTGCGC AACGCAAACG AACCAGCTCT AATATCGCAT CTCATACCAA TCCCTTGATT 
ACGGTGCACA GCTCTAGTTC GGCTCCGTAC CTGGCTGGAT CCAGTGTTTC CTTGAGAAGC 
GGCAACGGAG CCTTCGCAAC GGATTTGGAG CACAAACCGC TGAAGCCAGC GACAAAGGCT 
GCATCTAGTG CAAACCTGCG CGAAGGTGGA CCCTATTTGA AAAGCACGTT AGCTGAACTA 
CGAGCTGTTG GAGGTGAGGA GCCGGTGGCC AGTACTTCAA AGGCCTCTTC GATGAAGAGC 
TTGTCGCGGC AGAGCAGCGA AGAGGATTCC GCCAGCTGTT CCAGTCTCAA CGCAGAGCAG 
ACATCGGGGA TCATTCAAGT CTATACCGCC TACAGTACGG GTCTGGCCAG TGGAACCAGC 
TTAAAGATTC ACGTAACTCC GAAGACGACT GCCCGCGAAG TGATCAACCT GGTGGTGAAG 
CAACTTAACA TGGCCGTGGT TCTCAAAGGG AACAATGGCC CCATCTATGG ACCCGAGATG 
CTAGAGAACT TTTGCCTGGT TGCGGTGATT GGAGCAAGGG AACGCTGCCT GCGAGATGAC 
TTCAAACCGC TGCAACTGCA GAATCCCTGG AAGAAGGGCC GTCTGTACGT CCGCAAGAAG 
CATGAGCTGC TGGCGGCCAT CGAACACTCG AATCGGAAAT CGCATTTGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wake ( NM_001260315.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247244.1 Drosophila melanogaster wide awake (wake), transcript variant D, mRNA.

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CloneID	OFa05719
Clone ID Related Accession (Same CDS sequence)	NM_001260316.1 , NM_001260315.1
Accession Version	NM_001260315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wide awake, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACAGAT GCTACCACAG CGATAGCACT AAACTTCCCA ATATGCCGCA AAAGCTCCCG 
ATGAGCAGCT GTCTGCGTTC TCCGGCGGTC TTCGTTCGCC AGAAATCGTT TCACACGCTC 
AACGAGCTGA CCAGGTTCTG TCAATTGAAA GTGTCCCCAC ATCGGTCCAC CGCCACAGCC 
ACCTCCACAT CATCGTCACC ATCATCGTCG CCGGCAGCGG CGCAACAGCA GCGTCGCCAC 
AGTCACTATG CCATCGATGA CAACATATTG CCGCAAGATA AACGTGACGG TGACATCGAT 
AGTGTCAGGC TGCGGGAGAA GGTGGTTAAT CCCAGTGGCG GCAATAACAA TAACCAGGAC 
AACTATCCCA CCTATTATCC GGCACCATTG CTGTCCACCA GTCGCTGGTC GGAGCAGTTG 
ACACAGCATG CGGCAGTAGC CAAGATACGG ACAGCCATCT CCTGCCACTC CCAGGATCTC 
AGGGAAATGG AATTCCTACT GCCCCATTCG CGAACCGATT CCCAATCGAA TTCCCAATCG 
CAACTGTGCA TTTCGCGGCA CTCGCAGCCG AAAAATGCCA AGCTGCGGGA TGATCACGAT 
GAGACGGGCT CATCGGAATT GGATTCACCA CCGCTGGGTG ATGAGAATGC CTCGGCGAAT 
GGCCAACTTT CGGTGCACAG GCAGCCGGTA CAGCTGCGGA GTAAGATCCC GGGAAACACA 
CCTCATCCGC GGGCCAGCAA AATGTCCAAG AAACAGCTGA AGTTGGCCCA GGCCCAGCTG 
GACAAACTGA CGCAGAACAA TCTGCATCTG CATGCTCTCT TCTCGGCAGT GGAGCATGGT 
CACCTGGAGA AGGCGCGCAC TATTCTAGAG TCCACCGACG TAGATGTAAA CAGCATCAAT 
AACGATGGAC TATCTGCTCT GGACTTGGCG GTGTTAAGCA ATAATCGCTC CATGACCAGG 
ATGCTGCTTC AGCATGGCGC TGTGGAGGGC TCTCAGTTCT CTGTGGACAC CATTGGCACC 
AAACTGAACG GCTTGCTCAA GGACGCCGAG TCAAGAATTC ACGATCTAAG TGGACCGGAG 
GGCCTCTGTC CGCCGGTGTT TGCATCGCGT CCCTCCATCT CGAGCATCAT AATTGGCAAT 
TCCAGTGCAT CGGTAACTGG CTGCACGGGC AGCGAGGTGG AAAAACAGAT AGGCATCTGG 
GAGCGCCGAG TTAAGGGACT GCGTCGCTTG CAGCTCGGTT GGGATCAGGC CAGGCCGCCA 
GATGCACCCG CCTCCGTGGT CGTCGACGTC ACTGGCGACA ATTCCATCAG CGTACAAATC 
CTAGAGCCCT TCGAGGGGGC CATCGGCACG AAATTTAAAG TACAATGGTC GACCCGCGCG 
GATTTCAACA ACGTTGTGGG CGAGAGTGAG CTACTGGAAT GGATCAGCTT CCATGGCACG 
ATGGGTGCCC AGTGTCACAT ATCGGGCCTC ACCCAGGGTC GTCGCTACTT CCTGCGAGCC 
GCGTGCGGCA ATGTGAAGGG ATGGGGCACC TATCGAACCT CTGTGCCGGC TAGTGTAGTG 
CCCTCAACTT GGCGGGATTT AGACAATCGA GAGGACCGAT TCGTGGGTCG TCACCGAATC 
CTGGACAATC TATTTACCGC CGTGCGTTTG GCAAGACCCG CCGACGTATC CGAGCTGACT 
TTGGATCCTG CGAGCGCCCA ACGACGTAAT CCCAAAAAGA AGACTACCAT TAAGCAGTTG 
TTCTCGGTAA CCACCAAGTT TCAAAAGACA CTGAGACGCG GCATTTACTT CTCTTGTATT 
ATTCATTGCG ATGATAAAGT GCTGGTCACC AGCGAAGACT TTCCACCGGT CATCGAGGTG 
GATGAATCCT ATCCGAGTGC TTTGCACATG GATTACTACT GGCTCATGAA AGTTGCCTGC 
ACCTGGGAGG ATGTTAAGTC TCTACGTTCG GATATGGAGC GCAATCTTAC CTCCGCTGTT 
CACTTCCGCA CTAAGCTTCT ATCGGCGGTC TGTCAGATGC AATCGGCACT AGGCATTACG 
GACTTGGGCC AACTTTACTA TAAACCATTG CGGGATGCTC AGGGCACTGT GGTCCTAACC 
TGTGTGCAGT CGGTAAAGAG CCAAAAGGCC GTCTCCATCT TAAACTCGAG ATGGGTTCCA 
GTCAGCAAGC TGCAAAAGAA GCTAGGTGCT CTACACGAGG ACTACACCAT TAACGAGCTG 
CTCATCTCCT CGATTGGAGA TCAATTGCAC TATCAACAGG CGGCGCTGCA GCGTTTGGAG 
CCCGGTCTTT ACCTGGGCTA CCTAAAGATG CAATGCTCCA TAGACCAGAT CCAGGTGGTT 
GTGCCCGTGA AAACGCCGAA TGTCTTGCCC CACTGCAAGG TGCGCGAAAA CAGTCACATC 
ACGGCCGAAG AGTGGCAGGT GCTCCACCGT TACAGTAGCG ATCCTCTACG CCTGCCGTTG 
GACTTTAGCG CTCAAGGAGG AGATGGAGCC TCCGGAGCAA CAGCTACTAC GGAGGTGCAA 
CGCCTATTTT TATACGATCT CACCAATGCA ATGCACAAAC TGTTTGCCAG TATGAACATC 
AAAATCGCTG ATGCAACCAC TCACCGCTTG TACGACGTGG AGGTGATTGA GCATAGTCCG 
GATATTAGTT TCCTCGTGGT GTGTCCATCC GCCGAGTCCT CATGCGCTGT GCCCGGTCAG 
TCTGAGTTAC TGCTACAGCG AGATGACTTG GCCAGCTTGA GTATCCAGGC CTTTGAGATG 
ATTCACCTGC GCACTTATCA GCCGGCCATC ATTCAGAAGT ACGCCCGTTT ATCTTGCATC 
CTTGAATTGG ATACCGCACT GGCCACACAT TCGCTTCGCG AGGCATTCTC CAGCAGTGAA 
CTGCAGGCGG CCAAGGAACG TTTGGCCACG CTGCAGGAGC TCAGTGCGAG TCTTACGATT 
GTGTGGAAAA GTGTGCGATG GCTAATGGAT GTGGTGGCAT ATGCGCGAAA CAAAAACGCT 
CAACCCTCGT TGGCTATGCG AGAAATTCTG GACTTTGCAC AGCAAAGACA GGACGAAGCG 
GTAGCGACAT CGGCAGGAGG TTCCGCGAAC AAACAGCTGC TGCAGCTGCC CATCCGCGAG 
AGCAAGTTCT CCAAGACGGG CCAGGGAAGA GGCAGTTGGC CGGGACCGGG AACTAGTGAA 
GATCAATCGC AGAACAAGCC GGAGCACTCC AAGTCTGAAC AGAATCTGGA ACTGAGTGCT 
ATCACGCCAG TGGAACCTGC ATCCAATCAA GTTCCTACAC AGCAGCAGCA GCCGCAGCAG 
CAATCGCAGC AACAGCAACT CTTACAAGTT TCCAACATCA GCGAATACGC GGGATCGATT 
TGCTCAGAAG TATCATTTAG GAAGAATAGT GGCGATTCCA TGAGCTCCAC CTACACGTCC 
CGGAGCTTCT ACTCTGCCGT GGACTCAGCC TCAGATGGAA ATAGCACAAA CAGTGTGTTT 
GCCATTCCGC CCTCTCGTTC CGACGACACT CTGGCGGATG CACTGCGCCA CTCGCAAGCG 
GTAGCTGCGC AACGCAAACG AACCAGCTCT AATATCGCAT CTCATACCAA TCCCTTGATT 
ACGGTGCACA GCTCTAGTTC GGCTCCGTAC CTGGCTGGAT CCAGTGTTTC CTTGAGAAGC 
GGCAACGGAG CCTTCGCAAC GGATTTGGAG CACAAACCGC TGAAGCCAGC GACAAAGGCT 
GCATCTAGTG CAAACCTGCG CGAAGGTGGA CCCTATTTGA AAAGCACGTT AGCTGAACTA 
CGAGCTGTTG GAGGTGAGGA GCCGGTGGCC AGTACTTCAA AGGCCTCTTC GATGAAGAGC 
TTGTCGCGGC AGAGCAGCGA AGAGGATTCC GCCAGCTGTT CCAGTCTCAA CGCAGAGCAG 
ACATCGGGGA TCATTCAAGT CTATACCGCC TACAGTACGG GTCTGGCCAG TGGAACCAGC 
TTAAAGATTC ACGTAACTCC GAAGACGACT GCCCGCGAAG TGATCAACCT GGTGGTGAAG 
CAACTTAACA TGGCCGTGGT TCTCAAAGGG AACAATGGCC CCATCTATGG ACCCGAGATG 
CTAGAGAACT TTTGCCTGGT TGCGGTGATT GGAGCAAGGG AACGCTGCCT GCGAGATGAC 
TTCAAACCGC TGCAACTGCA GAATCCCTGG AAGAAGGGCC GTCTGTACGT CCGCAAGAAG 
CATGAGCTGC TGGCGGCCAT CGAACACTCG AATCGGAAAT CGCATTTGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247244.1
CDS	338..4591
Translation	MNRCYHSDSTKLPNMPQKLPMSSCLRSPAVFVRQKSFHTLNELTRFCQLKVSPHRSTATATSTSSSPSSSPAAAQQQRRHSHYAIDDNILPQDKRDGDIDSVRLREKVVNPSGGNNNNQDNYPTYYPAPLLSTSRWSEQLTQHAAVAKIRTAISCHSQDLREMEFLLPHSRTDSQSNSQSQLCISRHSQPKNAKLRDDHDETGSSELDSPPLGDENASANGQLSVHRQPVQLRSKIPGNTPHPRASKMSKKQLKLAQAQLDKLTQNNLHLHALFSAVEHGHLEKARTILESTDVDVNSINNDGLSALDLAVLSNNRSMTRMLLQHGAVEGSQFSVDTIGTKLNGLLKDAESRIHDLSGPEGLCPPVFASRPSISSIIIGNSSASVTGCTGSEVEKQIGIWERRVKGLRRLQLGWDQARPPDAPASVVVDVTGDNSISVQILEPFEGAIGTKFKVQWSTRADFNNVVGESELLEWISFHGTMGAQCHISGLTQGRRYFLRAACGNVKGWGTYRTSVPASVVPSTWRDLDNREDRFVGRHRILDNLFTAVRLARPADVSELTLDPASAQRRNPKKKTTIKQLFSVTTKFQKTLRRGIYFSCIIHCDDKVLVTSEDFPPVIEVDESYPSALHMDYYWLMKVACTWEDVKSLRSDMERNLTSAVHFRTKLLSAVCQMQSALGITDLGQLYYKPLRDAQGTVVLTCVQSVKSQKAVSILNSRWVPVSKLQKKLGALHEDYTINELLISSIGDQLHYQQAALQRLEPGLYLGYLKMQCSIDQIQVVVPVKTPNVLPHCKVRENSHITAEEWQVLHRYSSDPLRLPLDFSAQGGDGASGATATTEVQRLFLYDLTNAMHKLFASMNIKIADATTHRLYDVEVIEHSPDISFLVVCPSAESSCAVPGQSELLLQRDDLASLSIQAFEMIHLRTYQPAIIQKYARLSCILELDTALATHSLREAFSSSELQAAKERLATLQELSASLTIVWKSVRWLMDVVAYARNKNAQPSLAMREILDFAQQRQDEAVATSAGGSANKQLLQLPIRESKFSKTGQGRGSWPGPGTSEDQSQNKPEHSKSEQNLELSAITPVEPASNQVPTQQQQPQQQSQQQQLLQVSNISEYAGSICSEVSFRKNSGDSMSSTYTSRSFYSAVDSASDGNSTNSVFAIPPSRSDDTLADALRHSQAVAAQRKRTSSNIASHTNPLITVHSSSSAPYLAGSSVSLRSGNGAFATDLEHKPLKPATKAASSANLREGGPYLKSTLAELRAVGGEEPVASTSKASSMKSLSRQSSEEDSASCSSLNAEQTSGIIQVYTAYSTGLASGTSLKIHVTPKTTAREVINLVVKQLNMAVVLKGNNGPIYGPEMLENFCLVAVIGARERCLRDDFKPLQLQNPWKKGRLYVRKKHELLAAIEHSNRKSHLI

Target ORF information:

RefSeq Version	NM_001260315.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wide awake (wake), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260315.1

ORF Insert Sequence:

ATGAACAGAT GCTACCACAG CGATAGCACT AAACTTCCCA ATATGCCGCA AAAGCTCCCG 
ATGAGCAGCT GTCTGCGTTC TCCGGCGGTC TTCGTTCGCC AGAAATCGTT TCACACGCTC 
AACGAGCTGA CCAGGTTCTG TCAATTGAAA GTGTCCCCAC ATCGGTCCAC CGCCACAGCC 
ACCTCCACAT CATCGTCACC ATCATCGTCG CCGGCAGCGG CGCAACAGCA GCGTCGCCAC 
AGTCACTATG CCATCGATGA CAACATATTG CCGCAAGATA AACGTGACGG TGACATCGAT 
AGTGTCAGGC TGCGGGAGAA GGTGGTTAAT CCCAGTGGCG GCAATAACAA TAACCAGGAC 
AACTATCCCA CCTATTATCC GGCACCATTG CTGTCCACCA GTCGCTGGTC GGAGCAGTTG 
ACACAGCATG CGGCAGTAGC CAAGATACGG ACAGCCATCT CCTGCCACTC CCAGGATCTC 
AGGGAAATGG AATTCCTACT GCCCCATTCG CGAACCGATT CCCAATCGAA TTCCCAATCG 
CAACTGTGCA TTTCGCGGCA CTCGCAGCCG AAAAATGCCA AGCTGCGGGA TGATCACGAT 
GAGACGGGCT CATCGGAATT GGATTCACCA CCGCTGGGTG ATGAGAATGC CTCGGCGAAT 
GGCCAACTTT CGGTGCACAG GCAGCCGGTA CAGCTGCGGA GTAAGATCCC GGGAAACACA 
CCTCATCCGC GGGCCAGCAA AATGTCCAAG AAACAGCTGA AGTTGGCCCA GGCCCAGCTG 
GACAAACTGA CGCAGAACAA TCTGCATCTG CATGCTCTCT TCTCGGCAGT GGAGCATGGT 
CACCTGGAGA AGGCGCGCAC TATTCTAGAG TCCACCGACG TAGATGTAAA CAGCATCAAT 
AACGATGGAC TATCTGCTCT GGACTTGGCG GTGTTAAGCA ATAATCGCTC CATGACCAGG 
ATGCTGCTTC AGCATGGCGC TGTGGAGGGC TCTCAGTTCT CTGTGGACAC CATTGGCACC 
AAACTGAACG GCTTGCTCAA GGACGCCGAG TCAAGAATTC ACGATCTAAG TGGACCGGAG 
GGCCTCTGTC CGCCGGTGTT TGCATCGCGT CCCTCCATCT CGAGCATCAT AATTGGCAAT 
TCCAGTGCAT CGGTAACTGG CTGCACGGGC AGCGAGGTGG AAAAACAGAT AGGCATCTGG 
GAGCGCCGAG TTAAGGGACT GCGTCGCTTG CAGCTCGGTT GGGATCAGGC CAGGCCGCCA 
GATGCACCCG CCTCCGTGGT CGTCGACGTC ACTGGCGACA ATTCCATCAG CGTACAAATC 
CTAGAGCCCT TCGAGGGGGC CATCGGCACG AAATTTAAAG TACAATGGTC GACCCGCGCG 
GATTTCAACA ACGTTGTGGG CGAGAGTGAG CTACTGGAAT GGATCAGCTT CCATGGCACG 
ATGGGTGCCC AGTGTCACAT ATCGGGCCTC ACCCAGGGTC GTCGCTACTT CCTGCGAGCC 
GCGTGCGGCA ATGTGAAGGG ATGGGGCACC TATCGAACCT CTGTGCCGGC TAGTGTAGTG 
CCCTCAACTT GGCGGGATTT AGACAATCGA GAGGACCGAT TCGTGGGTCG TCACCGAATC 
CTGGACAATC TATTTACCGC CGTGCGTTTG GCAAGACCCG CCGACGTATC CGAGCTGACT 
TTGGATCCTG CGAGCGCCCA ACGACGTAAT CCCAAAAAGA AGACTACCAT TAAGCAGTTG 
TTCTCGGTAA CCACCAAGTT TCAAAAGACA CTGAGACGCG GCATTTACTT CTCTTGTATT 
ATTCATTGCG ATGATAAAGT GCTGGTCACC AGCGAAGACT TTCCACCGGT CATCGAGGTG 
GATGAATCCT ATCCGAGTGC TTTGCACATG GATTACTACT GGCTCATGAA AGTTGCCTGC 
ACCTGGGAGG ATGTTAAGTC TCTACGTTCG GATATGGAGC GCAATCTTAC CTCCGCTGTT 
CACTTCCGCA CTAAGCTTCT ATCGGCGGTC TGTCAGATGC AATCGGCACT AGGCATTACG 
GACTTGGGCC AACTTTACTA TAAACCATTG CGGGATGCTC AGGGCACTGT GGTCCTAACC 
TGTGTGCAGT CGGTAAAGAG CCAAAAGGCC GTCTCCATCT TAAACTCGAG ATGGGTTCCA 
GTCAGCAAGC TGCAAAAGAA GCTAGGTGCT CTACACGAGG ACTACACCAT TAACGAGCTG 
CTCATCTCCT CGATTGGAGA TCAATTGCAC TATCAACAGG CGGCGCTGCA GCGTTTGGAG 
CCCGGTCTTT ACCTGGGCTA CCTAAAGATG CAATGCTCCA TAGACCAGAT CCAGGTGGTT 
GTGCCCGTGA AAACGCCGAA TGTCTTGCCC CACTGCAAGG TGCGCGAAAA CAGTCACATC 
ACGGCCGAAG AGTGGCAGGT GCTCCACCGT TACAGTAGCG ATCCTCTACG CCTGCCGTTG 
GACTTTAGCG CTCAAGGAGG AGATGGAGCC TCCGGAGCAA CAGCTACTAC GGAGGTGCAA 
CGCCTATTTT TATACGATCT CACCAATGCA ATGCACAAAC TGTTTGCCAG TATGAACATC 
AAAATCGCTG ATGCAACCAC TCACCGCTTG TACGACGTGG AGGTGATTGA GCATAGTCCG 
GATATTAGTT TCCTCGTGGT GTGTCCATCC GCCGAGTCCT CATGCGCTGT GCCCGGTCAG 
TCTGAGTTAC TGCTACAGCG AGATGACTTG GCCAGCTTGA GTATCCAGGC CTTTGAGATG 
ATTCACCTGC GCACTTATCA GCCGGCCATC ATTCAGAAGT ACGCCCGTTT ATCTTGCATC 
CTTGAATTGG ATACCGCACT GGCCACACAT TCGCTTCGCG AGGCATTCTC CAGCAGTGAA 
CTGCAGGCGG CCAAGGAACG TTTGGCCACG CTGCAGGAGC TCAGTGCGAG TCTTACGATT 
GTGTGGAAAA GTGTGCGATG GCTAATGGAT GTGGTGGCAT ATGCGCGAAA CAAAAACGCT 
CAACCCTCGT TGGCTATGCG AGAAATTCTG GACTTTGCAC AGCAAAGACA GGACGAAGCG 
GTAGCGACAT CGGCAGGAGG TTCCGCGAAC AAACAGCTGC TGCAGCTGCC CATCCGCGAG 
AGCAAGTTCT CCAAGACGGG CCAGGGAAGA GGCAGTTGGC CGGGACCGGG AACTAGTGAA 
GATCAATCGC AGAACAAGCC GGAGCACTCC AAGTCTGAAC AGAATCTGGA ACTGAGTGCT 
ATCACGCCAG TGGAACCTGC ATCCAATCAA GTTCCTACAC AGCAGCAGCA GCCGCAGCAG 
CAATCGCAGC AACAGCAACT CTTACAAGTT TCCAACATCA GCGAATACGC GGGATCGATT 
TGCTCAGAAG TATCATTTAG GAAGAATAGT GGCGATTCCA TGAGCTCCAC CTACACGTCC 
CGGAGCTTCT ACTCTGCCGT GGACTCAGCC TCAGATGGAA ATAGCACAAA CAGTGTGTTT 
GCCATTCCGC CCTCTCGTTC CGACGACACT CTGGCGGATG CACTGCGCCA CTCGCAAGCG 
GTAGCTGCGC AACGCAAACG AACCAGCTCT AATATCGCAT CTCATACCAA TCCCTTGATT 
ACGGTGCACA GCTCTAGTTC GGCTCCGTAC CTGGCTGGAT CCAGTGTTTC CTTGAGAAGC 
GGCAACGGAG CCTTCGCAAC GGATTTGGAG CACAAACCGC TGAAGCCAGC GACAAAGGCT 
GCATCTAGTG CAAACCTGCG CGAAGGTGGA CCCTATTTGA AAAGCACGTT AGCTGAACTA 
CGAGCTGTTG GAGGTGAGGA GCCGGTGGCC AGTACTTCAA AGGCCTCTTC GATGAAGAGC 
TTGTCGCGGC AGAGCAGCGA AGAGGATTCC GCCAGCTGTT CCAGTCTCAA CGCAGAGCAG 
ACATCGGGGA TCATTCAAGT CTATACCGCC TACAGTACGG GTCTGGCCAG TGGAACCAGC 
TTAAAGATTC ACGTAACTCC GAAGACGACT GCCCGCGAAG TGATCAACCT GGTGGTGAAG 
CAACTTAACA TGGCCGTGGT TCTCAAAGGG AACAATGGCC CCATCTATGG ACCCGAGATG 
CTAGAGAACT TTTGCCTGGT TGCGGTGATT GGAGCAAGGG AACGCTGCCT GCGAGATGAC 
TTCAAACCGC TGCAACTGCA GAATCCCTGG AAGAAGGGCC GTCTGTACGT CCGCAAGAAG 
CATGAGCTGC TGGCGGCCAT CGAACACTCG AATCGGAAAT CGCATTTGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wake ( NM_001260314.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247243.1 Drosophila melanogaster wide awake (wake), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05721
Clone ID Related Accession (Same CDS sequence)	NM_001260314.3
Accession Version	NM_001260314.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wide awake, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260314.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCGTC GCAAGGCTCT CAGTTCGTAT GCCAAGTTCA AGGATCAGCA GGAGGCGGCG 
GCGGCAGTTA CCCCGCAGAA GGACAATGAG AGCGATGACT CGCTGCCCGC CTTGCGGCGC 
TCTCATTCCG TCCGTTCCTC TCTACGGCGG CTAAAAAACA AGCTGCACAG TCCCGCCCTA 
TCAATGCAGT CTCCCTTTAA GCTGCGCACC CAGCTGCACA TGCGCAATCG CAGCTCGCGA 
GGATCGGCCA CCTTGGACAA AAGCAAGGCG GCCAAGGCAC TACGCATGCT GGGCGATCTG 
GATGCAGTGG CTGTTCTGAC CACAAAAGTG AAGGGCAAGA ACGAATTTGT GCCGCTGAAA 
AGAGCAATGA ACTCAGATTC CTGTGATCAC GATGAACTTG GCATCGAAAG GCTCATGTCC 
TCCTTGCCAG TGGGTGCAAA GCTGCCTCGA AAGGCCTGCA AGTTGCTCCA GATCCCCGAG 
AGCTACTGCC GACAAGCCGA AGTCGCGCCG AAAGCCACAC TCGACTCGGA TCTGGAGAAG 
ACCTTCAGTG GCGAGGAATC CAGTGCCGTG CTGGCGGATA TCACTCGACA TGCCCAGCAG 
GGCATCTATG GAACGACACG CATGCGCACG GCGACAATTC GGAAGCCGAT GCCCTACCTG 
AACAGCCACA ACATATTGCC GCAAGATAAA CGTGACGGTG ACATCGATAG TGTCAGGCTG 
CGGGAGAAGG TGGTTAATCC CAGTGGCGGC AATAACAATA ACCAGGACAA CTATCCCACC 
TATTATCCGG CACCATTGCT GTCCACCAGT CGCTGGTCGG AGCAGTTGAC ACAGCATGCG 
GCAGTAGCCA AGATACGGAC AGCCATCTCC TGCCACTCCC AGGATCTCAG GGAAATGGAA 
TTCCTACTGC CCCATTCGCG AACCGATTCC CAATCGAATT CCCAATCGCA ACTGTGCATT 
TCGCGGCACT CGCAGCCGAA AAATGCCAAG CTGCGGGATG ATCACGATGA GACGGGCTCA 
TCGGAATTGG ATTCACCACC GCTGGGTGAT GAGAATGCCT CGGCGAATGG CCAACTTTCG 
GTGCACAGGC AGCCGGTACA GCTGCGGAGT AAGATCCCGG GAAACACACC TCATCCGCGG 
GCCAGCAAAA TGTCCAAGAA ACAGCTGAAG TTGGCCCAGG CCCAGCTGGA CAAACTGACG 
CAGAACAATC TGCATCTGCA TGCTCTCTTC TCGGCAGTGG AGCATGGTCA CCTGGAGAAG 
GCGCGCACTA TTCTAGAGTC CACCGACGTA GATGTAAACA GCATCAATAA CGATGGACTA 
TCTGCTCTGG ACTTGGCGGT GTTAAGCAAT AATCGCTCCA TGACCAGGAT GCTGCTTCAG 
CATGGCGCTG TGGAGGGCTC TCAGTTCTCT GTGGACACCA TTGGCACCAA ACTGAACGGC 
TTGCTCAAGG ACGCCGAGTC AAGAATTCAC GATCTAAGTG GACCGGAGGG CCTCTGTCCG 
CCGGTGTTTG CATCGCGTCC CTCCATCTCG AGCATCATAA TTGGCAATTC CAGTGCATCG 
GTAACTGGCT GCACGGGCAG CGAGGTGGAA AAACAGATAG GCATCTGGGA GCGCCGAGTT 
AAGGGACTGC GTCGCTTGCA GCTCGGTTGG GATCAGGCCA GGCCGCCAGA TGCACCCGCC 
TCCGTGGTCG TCGACGTCAC TGGCGACAAT TCCATCAGCG TACAAATCCT AGAGCCCTTC 
GAGGGGGCCA TCGGCACGAA ATTTAAAGTA CAATGGTCGA CCCGCGCGGA TTTCAACAAC 
GTTGTGGGCG AGAGTGAGCT ACTGGAATGG ATCAGCTTCC ATGGCACGAT GGGTGCCCAG 
TGTCACATAT CGGGCCTCAC CCAGGGTCGT CGCTACTTCC TGCGAGCCGC GTGCGGCAAT 
GTGAAGGGAT GGGGCACCTA TCGAACCTCT GTGCCGGCTA GTGTAGTGCC CTCAACTTGG 
CGGGATTTAG ACAATCGAGA GGACCGATTC GTGGGTCGTC ACCGAATCCT GGACAATCTA 
TTTACCGCCG TGCGTTTGGC AAGACCCGCC GACGTATCCG AGCTGACTTT GGATCCTGCG 
AGCGCCCAAC GACGTAATCC CAAAAAGAAG ACTACCATTA AGCAGTTGTT CTCGGTAACC 
ACCAAGTTTC AAAAGACACT GAGACGCGGC ATTTACTTCT CTTGTATTAT TCATTGCGAT 
GATAAAGTGC TGGTCACCAG CGAAGACTTT CCACCGGTCA TCGAGGTGGA TGAATCCTAT 
CCGAGTGCTT TGCACATGGA TTACTACTGG CTCATGAAAG TTGCCTGCAC CTGGGAGGAT 
GTTAAGTCTC TACGTTCGGA TATGGAGCGC AATCTTACCT CCGCTGTTCA CTTCCGCACT 
AAGCTTCTAT CGGCGGTCTG TCAGATGCAA TCGGCACTAG GCATTACGGA CTTGGGCCAA 
CTTTACTATA AACCATTGCG GGATGCTCAG GGCACTGTGG TCCTAACCTG TGTGCAGTCG 
GTAAAGAGCC AAAAGGCCGT CTCCATCTTA AACTCGAGAT GGGTTCCAGT CAGCAAGCTG 
CAAAAGAAGC TAGGTGCTCT ACACGAGGAC TACACCATTA ACGAGCTGCT CATCTCCTCG 
ATTGGAGATC AATTGCACTA TCAACAGGCG GCGCTGCAGC GTTTGGAGCC CGGTCTTTAC 
CTGGGCTACC TAAAGATGCA ATGCTCCATA GACCAGATCC AGGTGGTTGT GCCCGTGAAA 
ACGCCGAATG TCTTGCCCCA CTGCAAGGTG CGCGAAAACA GTCACATCAC GGCCGAAGAG 
TGGCAGGTGC TCCACCGTTA CAGTAGCGAT CCTCTACGCC TGCCGTTGGA CTTTAGCGCT 
CAAGGAGGAG ATGGAGCCTC CGGAGCAACA GCTACTACGG AGGTGCAACG CCTATTTTTA 
TACGATCTCA CCAATGCAAT GCACAAACTG TTTGCCAGTA TGAACATCAA AATCGCTGAT 
GCAACCACTC ACCGCTTGTA CGACGTGGAG GTGATTGAGC ATAGTCCGGA TATTAGTTTC 
CTCGTGGTGT GTCCATCCGC CGAGTCCTCA TGCGCTGTGC CCGGTCAGTC TGAGTTACTG 
CTACAGCGAG ATGACTTGGC CAGCTTGAGT ATCCAGGCCT TTGAGATGAT TCACCTGCGC 
ACTTATCAGC CGGCCATCAT TCAGAAGTAC GCCCGTTTAT CTTGCATCCT TGAATTGGAT 
ACCGCACTGG CCACACATTC GCTTCGCGAG GCATTCTCCA GCAGTGAACT GCAGGCGGCC 
AAGGAACGTT TGGCCACGCT GCAGGAGCTC AGTGCGAGTC TTACGATTGT GTGGAAAAGT 
GTGCGATGGC TAATGGATGT GGTGGCATAT GCGCGAAACA AAAACGCTCA ACCCTCGTTG 
GCTATGCGAG AAATTCTGGA CTTTGCACAG CAAAGACAGG ACGAAGCGGT AGCGACATCG 
GCAGGAGGTT CCGCGAACAA ACAGCTGCTG CAGCTGCCCA TCCGCGAGAG CAAGTTCTCC 
AAGACGGGCC AGGGAAGAGG CAGTTGGCCG GGACCGGGAA CTAGTGAAGA TCAATCGCAG 
AACAAGCCGG AGCACTCCAA GTCTGAACAG AATCTGGAAC TGAGTGCTAT CACGCCAGTG 
GAACCTGCAT CCAATCAAGT TCCTACACAG CAGCAGCAGC CGCAGCAGCA ATCGCAGCAA 
CAGCAACTCT TACAAGTTTC CAACATCAGC GAATACGCGG GATCGATTTG CTCAGAAGTA 
TCATTTAGGA AGAATAGTGG CGATTCCATG AGCTCCACCT ACACGTCCCG GAGCTTCTAC 
TCTGCCGTGG ACTCAGCCTC AGATGGAAAT AGCACAAACA GTGTGTTTGC CATTCCGCCC 
TCTCGTTCCG ACGACACTCT GGCGGATGCA CTGCGCCACT CGCAAGCGGT AGCTGCGCAA 
CGCAAACGAA CCAGCTCTAA TATCGCATCT CATACCAATC CCTTGATTAC GGTGCACAGC 
TCTAGTTCGG CTCCGTACCT GGCTGGATCC AGTGTTTCCT TGAGAAGCGG CAACGGAGCC 
TTCGCAACGG ATTTGGAGCA CAAACCGCTG AAGCCAGCGA CAAAGGCTGC ATCTAGTGCA 
AACCTGCGCG AAGGTGGACC CTATTTGAAA AGCACGTTAG CTGAACTACG AGCTGTTGGA 
GGTGAGGAGC CGGTGGCCAG TACTTCAAAG GCCTCTTCGA TGAAGAGCTT GTCGCGGCAG 
AGCAGCGAAG AGGATTCCGC CAGCTGTTCC AGTCTCAACG CAGAGCAGAC ATCGGGGATC 
ATTCAAGTCT ATACCGCCTA CAGTACGGGT CTGGCCAGTG GAACCAGCTT AAAGATTCAC 
GTAACTCCGA AGACGACTGC CCGCGAAGTG ATCAACCTGG TGGTGAAGCA ACTTAACATG 
GCCGTGGTTC TCAAAGGGAA CAATGGCCCC ATCTATGGAC CCGAGATGCT AGAGAACTTT 
TGCCTGGTTG CGGTGATTGG AGCAAGGGAA CGCTGCCTGC GAGATGACTT CAAACCGCTG 
CAACTGCAGA ATCCCTGGAA GAAGGGCCGT CTGTACGTCC GCAAGAAGCA TGAGCTGCTG 
GCGGCCATCG AACACTCGAA TCGGAAATCG CATTTGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247243.1
CDS	183..4844
Translation	MERRKALSSYAKFKDQQEAAAAVTPQKDNESDDSLPALRRSHSVRSSLRRLKNKLHSPALSMQSPFKLRTQLHMRNRSSRGSATLDKSKAAKALRMLGDLDAVAVLTTKVKGKNEFVPLKRAMNSDSCDHDELGIERLMSSLPVGAKLPRKACKLLQIPESYCRQAEVAPKATLDSDLEKTFSGEESSAVLADITRHAQQGIYGTTRMRTATIRKPMPYLNSHNILPQDKRDGDIDSVRLREKVVNPSGGNNNNQDNYPTYYPAPLLSTSRWSEQLTQHAAVAKIRTAISCHSQDLREMEFLLPHSRTDSQSNSQSQLCISRHSQPKNAKLRDDHDETGSSELDSPPLGDENASANGQLSVHRQPVQLRSKIPGNTPHPRASKMSKKQLKLAQAQLDKLTQNNLHLHALFSAVEHGHLEKARTILESTDVDVNSINNDGLSALDLAVLSNNRSMTRMLLQHGAVEGSQFSVDTIGTKLNGLLKDAESRIHDLSGPEGLCPPVFASRPSISSIIIGNSSASVTGCTGSEVEKQIGIWERRVKGLRRLQLGWDQARPPDAPASVVVDVTGDNSISVQILEPFEGAIGTKFKVQWSTRADFNNVVGESELLEWISFHGTMGAQCHISGLTQGRRYFLRAACGNVKGWGTYRTSVPASVVPSTWRDLDNREDRFVGRHRILDNLFTAVRLARPADVSELTLDPASAQRRNPKKKTTIKQLFSVTTKFQKTLRRGIYFSCIIHCDDKVLVTSEDFPPVIEVDESYPSALHMDYYWLMKVACTWEDVKSLRSDMERNLTSAVHFRTKLLSAVCQMQSALGITDLGQLYYKPLRDAQGTVVLTCVQSVKSQKAVSILNSRWVPVSKLQKKLGALHEDYTINELLISSIGDQLHYQQAALQRLEPGLYLGYLKMQCSIDQIQVVVPVKTPNVLPHCKVRENSHITAEEWQVLHRYSSDPLRLPLDFSAQGGDGASGATATTEVQRLFLYDLTNAMHKLFASMNIKIADATTHRLYDVEVIEHSPDISFLVVCPSAESSCAVPGQSELLLQRDDLASLSIQAFEMIHLRTYQPAIIQKYARLSCILELDTALATHSLREAFSSSELQAAKERLATLQELSASLTIVWKSVRWLMDVVAYARNKNAQPSLAMREILDFAQQRQDEAVATSAGGSANKQLLQLPIRESKFSKTGQGRGSWPGPGTSEDQSQNKPEHSKSEQNLELSAITPVEPASNQVPTQQQQPQQQSQQQQLLQVSNISEYAGSICSEVSFRKNSGDSMSSTYTSRSFYSAVDSASDGNSTNSVFAIPPSRSDDTLADALRHSQAVAAQRKRTSSNIASHTNPLITVHSSSSAPYLAGSSVSLRSGNGAFATDLEHKPLKPATKAASSANLREGGPYLKSTLAELRAVGGEEPVASTSKASSMKSLSRQSSEEDSASCSSLNAEQTSGIIQVYTAYSTGLASGTSLKIHVTPKTTAREVINLVVKQLNMAVVLKGNNGPIYGPEMLENFCLVAVIGARERCLRDDFKPLQLQNPWKKGRLYVRKKHELLAAIEHSNRKSHLI

Target ORF information:

RefSeq Version	NM_001260314.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wide awake (wake), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260314.3

ORF Insert Sequence:

ATGGAGCGTC GCAAGGCTCT CAGTTCGTAT GCCAAGTTCA AGGATCAGCA GGAGGCGGCG 
GCGGCAGTTA CCCCGCAGAA GGACAATGAG AGCGATGACT CGCTGCCCGC CTTGCGGCGC 
TCTCATTCCG TCCGTTCCTC TCTACGGCGG CTAAAAAACA AGCTGCACAG TCCCGCCCTA 
TCAATGCAGT CTCCCTTTAA GCTGCGCACC CAGCTGCACA TGCGCAATCG CAGCTCGCGA 
GGATCGGCCA CCTTGGACAA AAGCAAGGCG GCCAAGGCAC TACGCATGCT GGGCGATCTG 
GATGCAGTGG CTGTTCTGAC CACAAAAGTG AAGGGCAAGA ACGAATTTGT GCCGCTGAAA 
AGAGCAATGA ACTCAGATTC CTGTGATCAC GATGAACTTG GCATCGAAAG GCTCATGTCC 
TCCTTGCCAG TGGGTGCAAA GCTGCCTCGA AAGGCCTGCA AGTTGCTCCA GATCCCCGAG 
AGCTACTGCC GACAAGCCGA AGTCGCGCCG AAAGCCACAC TCGACTCGGA TCTGGAGAAG 
ACCTTCAGTG GCGAGGAATC CAGTGCCGTG CTGGCGGATA TCACTCGACA TGCCCAGCAG 
GGCATCTATG GAACGACACG CATGCGCACG GCGACAATTC GGAAGCCGAT GCCCTACCTG 
AACAGCCACA ACATATTGCC GCAAGATAAA CGTGACGGTG ACATCGATAG TGTCAGGCTG 
CGGGAGAAGG TGGTTAATCC CAGTGGCGGC AATAACAATA ACCAGGACAA CTATCCCACC 
TATTATCCGG CACCATTGCT GTCCACCAGT CGCTGGTCGG AGCAGTTGAC ACAGCATGCG 
GCAGTAGCCA AGATACGGAC AGCCATCTCC TGCCACTCCC AGGATCTCAG GGAAATGGAA 
TTCCTACTGC CCCATTCGCG AACCGATTCC CAATCGAATT CCCAATCGCA ACTGTGCATT 
TCGCGGCACT CGCAGCCGAA AAATGCCAAG CTGCGGGATG ATCACGATGA GACGGGCTCA 
TCGGAATTGG ATTCACCACC GCTGGGTGAT GAGAATGCCT CGGCGAATGG CCAACTTTCG 
GTGCACAGGC AGCCGGTACA GCTGCGGAGT AAGATCCCGG GAAACACACC TCATCCGCGG 
GCCAGCAAAA TGTCCAAGAA ACAGCTGAAG TTGGCCCAGG CCCAGCTGGA CAAACTGACG 
CAGAACAATC TGCATCTGCA TGCTCTCTTC TCGGCAGTGG AGCATGGTCA CCTGGAGAAG 
GCGCGCACTA TTCTAGAGTC CACCGACGTA GATGTAAACA GCATCAATAA CGATGGACTA 
TCTGCTCTGG ACTTGGCGGT GTTAAGCAAT AATCGCTCCA TGACCAGGAT GCTGCTTCAG 
CATGGCGCTG TGGAGGGCTC TCAGTTCTCT GTGGACACCA TTGGCACCAA ACTGAACGGC 
TTGCTCAAGG ACGCCGAGTC AAGAATTCAC GATCTAAGTG GACCGGAGGG CCTCTGTCCG 
CCGGTGTTTG CATCGCGTCC CTCCATCTCG AGCATCATAA TTGGCAATTC CAGTGCATCG 
GTAACTGGCT GCACGGGCAG CGAGGTGGAA AAACAGATAG GCATCTGGGA GCGCCGAGTT 
AAGGGACTGC GTCGCTTGCA GCTCGGTTGG GATCAGGCCA GGCCGCCAGA TGCACCCGCC 
TCCGTGGTCG TCGACGTCAC TGGCGACAAT TCCATCAGCG TACAAATCCT AGAGCCCTTC 
GAGGGGGCCA TCGGCACGAA ATTTAAAGTA CAATGGTCGA CCCGCGCGGA TTTCAACAAC 
GTTGTGGGCG AGAGTGAGCT ACTGGAATGG ATCAGCTTCC ATGGCACGAT GGGTGCCCAG 
TGTCACATAT CGGGCCTCAC CCAGGGTCGT CGCTACTTCC TGCGAGCCGC GTGCGGCAAT 
GTGAAGGGAT GGGGCACCTA TCGAACCTCT GTGCCGGCTA GTGTAGTGCC CTCAACTTGG 
CGGGATTTAG ACAATCGAGA GGACCGATTC GTGGGTCGTC ACCGAATCCT GGACAATCTA 
TTTACCGCCG TGCGTTTGGC AAGACCCGCC GACGTATCCG AGCTGACTTT GGATCCTGCG 
AGCGCCCAAC GACGTAATCC CAAAAAGAAG ACTACCATTA AGCAGTTGTT CTCGGTAACC 
ACCAAGTTTC AAAAGACACT GAGACGCGGC ATTTACTTCT CTTGTATTAT TCATTGCGAT 
GATAAAGTGC TGGTCACCAG CGAAGACTTT CCACCGGTCA TCGAGGTGGA TGAATCCTAT 
CCGAGTGCTT TGCACATGGA TTACTACTGG CTCATGAAAG TTGCCTGCAC CTGGGAGGAT 
GTTAAGTCTC TACGTTCGGA TATGGAGCGC AATCTTACCT CCGCTGTTCA CTTCCGCACT 
AAGCTTCTAT CGGCGGTCTG TCAGATGCAA TCGGCACTAG GCATTACGGA CTTGGGCCAA 
CTTTACTATA AACCATTGCG GGATGCTCAG GGCACTGTGG TCCTAACCTG TGTGCAGTCG 
GTAAAGAGCC AAAAGGCCGT CTCCATCTTA AACTCGAGAT GGGTTCCAGT CAGCAAGCTG 
CAAAAGAAGC TAGGTGCTCT ACACGAGGAC TACACCATTA ACGAGCTGCT CATCTCCTCG 
ATTGGAGATC AATTGCACTA TCAACAGGCG GCGCTGCAGC GTTTGGAGCC CGGTCTTTAC 
CTGGGCTACC TAAAGATGCA ATGCTCCATA GACCAGATCC AGGTGGTTGT GCCCGTGAAA 
ACGCCGAATG TCTTGCCCCA CTGCAAGGTG CGCGAAAACA GTCACATCAC GGCCGAAGAG 
TGGCAGGTGC TCCACCGTTA CAGTAGCGAT CCTCTACGCC TGCCGTTGGA CTTTAGCGCT 
CAAGGAGGAG ATGGAGCCTC CGGAGCAACA GCTACTACGG AGGTGCAACG CCTATTTTTA 
TACGATCTCA CCAATGCAAT GCACAAACTG TTTGCCAGTA TGAACATCAA AATCGCTGAT 
GCAACCACTC ACCGCTTGTA CGACGTGGAG GTGATTGAGC ATAGTCCGGA TATTAGTTTC 
CTCGTGGTGT GTCCATCCGC CGAGTCCTCA TGCGCTGTGC CCGGTCAGTC TGAGTTACTG 
CTACAGCGAG ATGACTTGGC CAGCTTGAGT ATCCAGGCCT TTGAGATGAT TCACCTGCGC 
ACTTATCAGC CGGCCATCAT TCAGAAGTAC GCCCGTTTAT CTTGCATCCT TGAATTGGAT 
ACCGCACTGG CCACACATTC GCTTCGCGAG GCATTCTCCA GCAGTGAACT GCAGGCGGCC 
AAGGAACGTT TGGCCACGCT GCAGGAGCTC AGTGCGAGTC TTACGATTGT GTGGAAAAGT 
GTGCGATGGC TAATGGATGT GGTGGCATAT GCGCGAAACA AAAACGCTCA ACCCTCGTTG 
GCTATGCGAG AAATTCTGGA CTTTGCACAG CAAAGACAGG ACGAAGCGGT AGCGACATCG 
GCAGGAGGTT CCGCGAACAA ACAGCTGCTG CAGCTGCCCA TCCGCGAGAG CAAGTTCTCC 
AAGACGGGCC AGGGAAGAGG CAGTTGGCCG GGACCGGGAA CTAGTGAAGA TCAATCGCAG 
AACAAGCCGG AGCACTCCAA GTCTGAACAG AATCTGGAAC TGAGTGCTAT CACGCCAGTG 
GAACCTGCAT CCAATCAAGT TCCTACACAG CAGCAGCAGC CGCAGCAGCA ATCGCAGCAA 
CAGCAACTCT TACAAGTTTC CAACATCAGC GAATACGCGG GATCGATTTG CTCAGAAGTA 
TCATTTAGGA AGAATAGTGG CGATTCCATG AGCTCCACCT ACACGTCCCG GAGCTTCTAC 
TCTGCCGTGG ACTCAGCCTC AGATGGAAAT AGCACAAACA GTGTGTTTGC CATTCCGCCC 
TCTCGTTCCG ACGACACTCT GGCGGATGCA CTGCGCCACT CGCAAGCGGT AGCTGCGCAA 
CGCAAACGAA CCAGCTCTAA TATCGCATCT CATACCAATC CCTTGATTAC GGTGCACAGC 
TCTAGTTCGG CTCCGTACCT GGCTGGATCC AGTGTTTCCT TGAGAAGCGG CAACGGAGCC 
TTCGCAACGG ATTTGGAGCA CAAACCGCTG AAGCCAGCGA CAAAGGCTGC ATCTAGTGCA 
AACCTGCGCG AAGGTGGACC CTATTTGAAA AGCACGTTAG CTGAACTACG AGCTGTTGGA 
GGTGAGGAGC CGGTGGCCAG TACTTCAAAG GCCTCTTCGA TGAAGAGCTT GTCGCGGCAG 
AGCAGCGAAG AGGATTCCGC CAGCTGTTCC AGTCTCAACG CAGAGCAGAC ATCGGGGATC 
ATTCAAGTCT ATACCGCCTA CAGTACGGGT CTGGCCAGTG GAACCAGCTT AAAGATTCAC 
GTAACTCCGA AGACGACTGC CCGCGAAGTG ATCAACCTGG TGGTGAAGCA ACTTAACATG 
GCCGTGGTTC TCAAAGGGAA CAATGGCCCC ATCTATGGAC CCGAGATGCT AGAGAACTTT 
TGCCTGGTTG CGGTGATTGG AGCAAGGGAA CGCTGCCTGC GAGATGACTT CAAACCGCTG 
CAACTGCAGA ATCCCTGGAA GAAGGGCCGT CTGTACGTCC GCAAGAAGCA TGAGCTGCTG 
GCGGCCATCG AACACTCGAA TCGGAAATCG CATTTGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wake ( NM_001300541.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287470.1 Drosophila melanogaster wide awake (wake), transcript variant H, mRNA.

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CloneID	OFa22136
Clone ID Related Accession (Same CDS sequence)	NM_001300541.1
Accession Version	NM_001300541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wide awake, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCAAA AGCTCCCGAT GAGCAGCTGT CTGCGTTCTC CGGCGGTCTT CGTTCGCCAG 
AAATCGTTTC ACACGCTCAA CGAGCTGACC AGGTTCTGTC AATTGAAAGT GTCCCCACAT 
CGGTCCACCG CCACAGCCAC CTCCACATCA TCGTCACCAT CATCGTCGCC GGCAGCGGCG 
CAACAGCAGC GTCGCCACAG TCACTATGCC ATCGATGACA ACATATTGCC GCAAGATAAA 
CGTGACGGTG ACATCGATAG TGTCAGGCTG CGGGAGAAGG TGGTTAATCC CAGTGGCGGC 
AATAACAATA ACCAGGACAA CTATCCCACC TATTATCCGG CACCATTGCT GTCCACCAGT 
CGCTGGTCGG AGCAGTTGAC ACAGCATGCG GCAGTAGCCA AGATACGGAC AGCCATCTCC 
TGCCACTCCC AGGATCTCAG GGAAATGGAA TTCCTACTGC CCCATTCGCG AACCGATTCC 
CAATCGAATT CCCAATCGCA ACTGTGCATT TCGCGGCACT CGCAGCCGAA AAATGCCAAG 
CTGCGGGATG ATCACGATGA GACGGGCTCA TCGGAATTGG ATTCACCACC GCTGGGTGAT 
GAGAATGCCT CGGCGAATGG CCAACTTTCG GTGCACAGGC AGCCGGTACA GCTGCGGAGT 
AAGATCCCGG GAAACACACC TCATCCGCGG GCCAGCAAAA TGTCCAAGAA ACAGCTGAAG 
TTGGCCCAGG CCCAGCTGGA CAAACTGACG CAGAACAATC TGCATCTGCA TGCTCTCTTC 
TCGGCAGTGG AGCATGGTCA CCTGGAGAAG GCGCGCACTA TTCTAGAGTC CACCGACGTA 
GATGTAAACA GCATCAATAA CGATGGACTA TCTGCTCTGG ACTTGGCGGT GTTAAGCAAT 
AATCGCTCCA TGACCAGGAT GCTGCTTCAG CATGGCGCTG TGGAGGGCTC TCAGTTCTCT 
GTGGACACCA TTGGCACCAA ACTGAACGGC TTGCTCAAGG ACGCCGAGTC AAGAATTCAC 
GATCTAAGTG GACCGGAGGG CCTCTGTCCG CCGGTGTTTG CATCGCGTCC CTCCATCTCG 
AGCATCATAA TTGGCAATTC CAGTGCATCG GTAACTGGCT GCACGGGCAG CGAGGTGGAA 
AAACAGATAG GCATCTGGGA GCGCCGAGTT AAGGGACTGC GTCGCTTGCA GCTCGGTTGG 
GATCAGGCCA GGCCGCCAGA TGCACCCGCC TCCGTGGTCG TCGACGTCAC TGGCGACAAT 
TCCATCAGCG TACAAATCCT AGAGCCCTTC GAGGGGGCCA TCGGCACGAA ATTTAAAGTA 
CAATGGTCGA CCCGCGCGGA TTTCAACAAC GTTGTGGGCG AGAGTGAGCT ACTGGAATGG 
ATCAGCTTCC ATGGCACGAT GGGTGCCCAG TGTCACATAT CGGGCCTCAC CCAGGGTCGT 
CGCTACTTCC TGCGAGCCGC GTGCGGCAAT GTGAAGGGAT GGGGCACCTA TCGAACCTCT 
GTGCCGGCTA GTGTAGTGCC CTCAACTTGG CGGGATTTAG ACAATCGAGA GGACCGATTC 
GTGGGTCGTC ACCGAATCCT GGACAATCTA TTTACCGCCG TGCGTTTGGC AAGACCCGCC 
GACGTATCCG AGCTGACTTT GGATCCTGCG AGCGCCCAAC GACGTAATCC CAAAAAGAAG 
ACTACCATTA AGCAGTTGTT CTCGGTAACC ACCAAGTTTC AAAAGACACT GAGACGCGGC 
ATTTACTTCT CTTGTATTAT TCATTGCGAT GATAAAGTGC TGGTCACCAG CGAAGACTTT 
CCACCGGTCA TCGAGGTGGA TGAATCCTAT CCGAGTGCTT TGCACATGGA TTACTACTGG 
CTCATGAAAG TTGCCTGCAC CTGGGAGGAT GTTAAGTCTC TACGTTCGGA TATGGAGCGC 
AATCTTACCT CCGCTGTTCA CTTCCGCACT AAGCTTCTAT CGGCGGTCTG TCAGATGCAA 
TCGGCACTAG GCATTACGGA CTTGGGCCAA CTTTACTATA AACCATTGCG GGATGCTCAG 
GGCACTGTGG TCCTAACCTG TGTGCAGTCG GTAAAGAGCC AAAAGGCCGT CTCCATCTTA 
AACTCGAGAT GGGTTCCAGT CAGCAAGCTG CAAAAGAAGC TAGGTGCTCT ACACGAGGAC 
TACACCATTA ACGAGCTGCT CATCTCCTCG ATTGGAGATC AATTGCACTA TCAACAGGCG 
GCGCTGCAGC GTTTGGAGCC CGGTCTTTAC CTGGGCTACC TAAAGATGCA ATGCTCCATA 
GACCAGATCC AGGTGGTTGT GCCCGTGAAA ACGCCGAATG TCTTGCCCCA CTGCAAGGTG 
CGCGAAAACA GTCACATCAC GGCCGAAGAG TGGCAGGTGC TCCACCGTTA CAGTAGCGAT 
CCTCTACGCC TGCCGTTGGA CTTTAGCGCT CAAGGAGGAG ATGGAGCCTC CGGAGCAACA 
GCTACTACGG AGGTGCAACG CCTATTTTTA TACGATCTCA CCAATGCAAT GCACAAACTG 
TTTGCCAGTA TGAACATCAA AATCGCTGAT GCAACCACTC ACCGCTTGTA CGACGTGGAG 
GTGATTGAGC ATAGTCCGGA TATTAGTTTC CTCGTGGTGT GTCCATCCGC CGAGTCCTCA 
TGCGCTGTGC CCGGTCAGTC TGAGTTACTG CTACAGCGAG ATGACTTGGC CAGCTTGAGT 
ATCCAGGCCT TTGAGATGAT TCACCTGCGC ACTTATCAGC CGGCCATCAT TCAGAAGTAC 
GCCCGTTTAT CTTGCATCCT TGAATTGGAT ACCGCACTGG CCACACATTC GCTTCGCGAG 
GCATTCTCCA GCAGTGAACT GCAGGCGGCC AAGGAACGTT TGGCCACGCT GCAGGAGCTC 
AGTGCGAGTC TTACGATTGT GTGGAAAAGT GTGCGATGGC TAATGGATGT GGTGGCATAT 
GCGCGAAACA AAAACGCTCA ACCCTCGTTG GCTATGCGAG AAATTCTGGA CTTTGCACAG 
CAAAGACAGG ACGAAGCGGT AGCGACATCG GCAGGAGGTT CCGCGAACAA ACAGCTGCTG 
CAGCTGCCCA TCCGCGAGAG CAAGTTCTCC AAGACGGGCC AGGGAAGAGG CAGTTGGCCG 
GGACCGGGAA CTAGTGAAGA TCAATCGCAG AACAAGCCGG AGCACTCCAA GTCTGAACAG 
AATCTGGAAC TGAGTGCTAT CACGCCAGTG GAACCTGCAT CCAATCAAGT TCCTACACAG 
CAGCAGCAGC CGCAGCAGCA ATCGCAGCAA CAGCAACTCT TACAAGTTTC CAACATCAGC 
GAATACGCGG GATCGATTTG CTCAGAAGTA TCATTTAGGA AGAATAGTGG CGATTCCATG 
AGCTCCACCT ACACGTCCCG GAGCTTCTAC TCTGCCGTGG ACTCAGCCTC AGATGGAAAT 
AGCACAAACA GTGTGTTTGC CATTCCGCCC TCTCGTTCCG ACGACACTCT GGCGGATGCA 
CTGCGCCACT CGCAAGCGGT AGCTGCGCAA CGCAAACGAA CCAGCTCTAA TATCGCATCT 
CATACCAATC CCTTGATTAC GGTGCACAGC TCTAGTTCGG CTCCGTACCT GGCTGGATCC 
AGTGTTTCCT TGAGAAGCGG CAACGGAGCC TTCGCAACGG ATTTGGAGCA CAAACCGCTG 
AAGCCAGCGA CAAAGGCTGC ATCTAGTGCA AACCTGCGCG AAGGTGGACC CTATTTGAAA 
AGCACGTTAG CTGAACTACG AGCTGTTGGA GGTGAGGAGC CGGTGGCCAG TACTTCAAAG 
GCCTCTTCGA TGAAGAGCTT GTCGCGGCAG AGCAGCGAAG AGGATTCCGC CAGCTGTTCC 
AGTCTCAACG CAGAGCAGAC ATCGGGGATC ATTCAAGTCT ATACCGCCTA CAGTACGGGT 
CTGGCCAGTG GAACCAGCTT AAAGATTCAC GTAACTCCGA AGACGACTGC CCGCGAAGTG 
ATCAACCTGG TGGTGAAGCA ACTTAACATG GCCGTGGTTC TCAAAGGGAA CAATGGCCCC 
ATCTATGGAC CCGAGATGCT AGAGAACTTT TGCCTGGTTG CGGTGATTGG AGCAAGGGAA 
CGCTGCCTGC GAGATGACTT CAAACCGCTG CAACTGCAGA ATCCCTGGAA GAAGGGCCGT 
CTGTACGTCC GCAAGAAGCA TGAGCTGCTG GCGGCCATCG AACACTCGAA TCGGAAATCG 
CATTTGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287470.1
CDS	1915..6126
Translation	MPQKLPMSSCLRSPAVFVRQKSFHTLNELTRFCQLKVSPHRSTATATSTSSSPSSSPAAAQQQRRHSHYAIDDNILPQDKRDGDIDSVRLREKVVNPSGGNNNNQDNYPTYYPAPLLSTSRWSEQLTQHAAVAKIRTAISCHSQDLREMEFLLPHSRTDSQSNSQSQLCISRHSQPKNAKLRDDHDETGSSELDSPPLGDENASANGQLSVHRQPVQLRSKIPGNTPHPRASKMSKKQLKLAQAQLDKLTQNNLHLHALFSAVEHGHLEKARTILESTDVDVNSINNDGLSALDLAVLSNNRSMTRMLLQHGAVEGSQFSVDTIGTKLNGLLKDAESRIHDLSGPEGLCPPVFASRPSISSIIIGNSSASVTGCTGSEVEKQIGIWERRVKGLRRLQLGWDQARPPDAPASVVVDVTGDNSISVQILEPFEGAIGTKFKVQWSTRADFNNVVGESELLEWISFHGTMGAQCHISGLTQGRRYFLRAACGNVKGWGTYRTSVPASVVPSTWRDLDNREDRFVGRHRILDNLFTAVRLARPADVSELTLDPASAQRRNPKKKTTIKQLFSVTTKFQKTLRRGIYFSCIIHCDDKVLVTSEDFPPVIEVDESYPSALHMDYYWLMKVACTWEDVKSLRSDMERNLTSAVHFRTKLLSAVCQMQSALGITDLGQLYYKPLRDAQGTVVLTCVQSVKSQKAVSILNSRWVPVSKLQKKLGALHEDYTINELLISSIGDQLHYQQAALQRLEPGLYLGYLKMQCSIDQIQVVVPVKTPNVLPHCKVRENSHITAEEWQVLHRYSSDPLRLPLDFSAQGGDGASGATATTEVQRLFLYDLTNAMHKLFASMNIKIADATTHRLYDVEVIEHSPDISFLVVCPSAESSCAVPGQSELLLQRDDLASLSIQAFEMIHLRTYQPAIIQKYARLSCILELDTALATHSLREAFSSSELQAAKERLATLQELSASLTIVWKSVRWLMDVVAYARNKNAQPSLAMREILDFAQQRQDEAVATSAGGSANKQLLQLPIRESKFSKTGQGRGSWPGPGTSEDQSQNKPEHSKSEQNLELSAITPVEPASNQVPTQQQQPQQQSQQQQLLQVSNISEYAGSICSEVSFRKNSGDSMSSTYTSRSFYSAVDSASDGNSTNSVFAIPPSRSDDTLADALRHSQAVAAQRKRTSSNIASHTNPLITVHSSSSAPYLAGSSVSLRSGNGAFATDLEHKPLKPATKAASSANLREGGPYLKSTLAELRAVGGEEPVASTSKASSMKSLSRQSSEEDSASCSSLNAEQTSGIIQVYTAYSTGLASGTSLKIHVTPKTTAREVINLVVKQLNMAVVLKGNNGPIYGPEMLENFCLVAVIGARERCLRDDFKPLQLQNPWKKGRLYVRKKHELLAAIEHSNRKSHLI

Target ORF information:

RefSeq Version	NM_001300541.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wide awake (wake), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300541.1

ORF Insert Sequence:

ATGCCGCAAA AGCTCCCGAT GAGCAGCTGT CTGCGTTCTC CGGCGGTCTT CGTTCGCCAG 
AAATCGTTTC ACACGCTCAA CGAGCTGACC AGGTTCTGTC AATTGAAAGT GTCCCCACAT 
CGGTCCACCG CCACAGCCAC CTCCACATCA TCGTCACCAT CATCGTCGCC GGCAGCGGCG 
CAACAGCAGC GTCGCCACAG TCACTATGCC ATCGATGACA ACATATTGCC GCAAGATAAA 
CGTGACGGTG ACATCGATAG TGTCAGGCTG CGGGAGAAGG TGGTTAATCC CAGTGGCGGC 
AATAACAATA ACCAGGACAA CTATCCCACC TATTATCCGG CACCATTGCT GTCCACCAGT 
CGCTGGTCGG AGCAGTTGAC ACAGCATGCG GCAGTAGCCA AGATACGGAC AGCCATCTCC 
TGCCACTCCC AGGATCTCAG GGAAATGGAA TTCCTACTGC CCCATTCGCG AACCGATTCC 
CAATCGAATT CCCAATCGCA ACTGTGCATT TCGCGGCACT CGCAGCCGAA AAATGCCAAG 
CTGCGGGATG ATCACGATGA GACGGGCTCA TCGGAATTGG ATTCACCACC GCTGGGTGAT 
GAGAATGCCT CGGCGAATGG CCAACTTTCG GTGCACAGGC AGCCGGTACA GCTGCGGAGT 
AAGATCCCGG GAAACACACC TCATCCGCGG GCCAGCAAAA TGTCCAAGAA ACAGCTGAAG 
TTGGCCCAGG CCCAGCTGGA CAAACTGACG CAGAACAATC TGCATCTGCA TGCTCTCTTC 
TCGGCAGTGG AGCATGGTCA CCTGGAGAAG GCGCGCACTA TTCTAGAGTC CACCGACGTA 
GATGTAAACA GCATCAATAA CGATGGACTA TCTGCTCTGG ACTTGGCGGT GTTAAGCAAT 
AATCGCTCCA TGACCAGGAT GCTGCTTCAG CATGGCGCTG TGGAGGGCTC TCAGTTCTCT 
GTGGACACCA TTGGCACCAA ACTGAACGGC TTGCTCAAGG ACGCCGAGTC AAGAATTCAC 
GATCTAAGTG GACCGGAGGG CCTCTGTCCG CCGGTGTTTG CATCGCGTCC CTCCATCTCG 
AGCATCATAA TTGGCAATTC CAGTGCATCG GTAACTGGCT GCACGGGCAG CGAGGTGGAA 
AAACAGATAG GCATCTGGGA GCGCCGAGTT AAGGGACTGC GTCGCTTGCA GCTCGGTTGG 
GATCAGGCCA GGCCGCCAGA TGCACCCGCC TCCGTGGTCG TCGACGTCAC TGGCGACAAT 
TCCATCAGCG TACAAATCCT AGAGCCCTTC GAGGGGGCCA TCGGCACGAA ATTTAAAGTA 
CAATGGTCGA CCCGCGCGGA TTTCAACAAC GTTGTGGGCG AGAGTGAGCT ACTGGAATGG 
ATCAGCTTCC ATGGCACGAT GGGTGCCCAG TGTCACATAT CGGGCCTCAC CCAGGGTCGT 
CGCTACTTCC TGCGAGCCGC GTGCGGCAAT GTGAAGGGAT GGGGCACCTA TCGAACCTCT 
GTGCCGGCTA GTGTAGTGCC CTCAACTTGG CGGGATTTAG ACAATCGAGA GGACCGATTC 
GTGGGTCGTC ACCGAATCCT GGACAATCTA TTTACCGCCG TGCGTTTGGC AAGACCCGCC 
GACGTATCCG AGCTGACTTT GGATCCTGCG AGCGCCCAAC GACGTAATCC CAAAAAGAAG 
ACTACCATTA AGCAGTTGTT CTCGGTAACC ACCAAGTTTC AAAAGACACT GAGACGCGGC 
ATTTACTTCT CTTGTATTAT TCATTGCGAT GATAAAGTGC TGGTCACCAG CGAAGACTTT 
CCACCGGTCA TCGAGGTGGA TGAATCCTAT CCGAGTGCTT TGCACATGGA TTACTACTGG 
CTCATGAAAG TTGCCTGCAC CTGGGAGGAT GTTAAGTCTC TACGTTCGGA TATGGAGCGC 
AATCTTACCT CCGCTGTTCA CTTCCGCACT AAGCTTCTAT CGGCGGTCTG TCAGATGCAA 
TCGGCACTAG GCATTACGGA CTTGGGCCAA CTTTACTATA AACCATTGCG GGATGCTCAG 
GGCACTGTGG TCCTAACCTG TGTGCAGTCG GTAAAGAGCC AAAAGGCCGT CTCCATCTTA 
AACTCGAGAT GGGTTCCAGT CAGCAAGCTG CAAAAGAAGC TAGGTGCTCT ACACGAGGAC 
TACACCATTA ACGAGCTGCT CATCTCCTCG ATTGGAGATC AATTGCACTA TCAACAGGCG 
GCGCTGCAGC GTTTGGAGCC CGGTCTTTAC CTGGGCTACC TAAAGATGCA ATGCTCCATA 
GACCAGATCC AGGTGGTTGT GCCCGTGAAA ACGCCGAATG TCTTGCCCCA CTGCAAGGTG 
CGCGAAAACA GTCACATCAC GGCCGAAGAG TGGCAGGTGC TCCACCGTTA CAGTAGCGAT 
CCTCTACGCC TGCCGTTGGA CTTTAGCGCT CAAGGAGGAG ATGGAGCCTC CGGAGCAACA 
GCTACTACGG AGGTGCAACG CCTATTTTTA TACGATCTCA CCAATGCAAT GCACAAACTG 
TTTGCCAGTA TGAACATCAA AATCGCTGAT GCAACCACTC ACCGCTTGTA CGACGTGGAG 
GTGATTGAGC ATAGTCCGGA TATTAGTTTC CTCGTGGTGT GTCCATCCGC CGAGTCCTCA 
TGCGCTGTGC CCGGTCAGTC TGAGTTACTG CTACAGCGAG ATGACTTGGC CAGCTTGAGT 
ATCCAGGCCT TTGAGATGAT TCACCTGCGC ACTTATCAGC CGGCCATCAT TCAGAAGTAC 
GCCCGTTTAT CTTGCATCCT TGAATTGGAT ACCGCACTGG CCACACATTC GCTTCGCGAG 
GCATTCTCCA GCAGTGAACT GCAGGCGGCC AAGGAACGTT TGGCCACGCT GCAGGAGCTC 
AGTGCGAGTC TTACGATTGT GTGGAAAAGT GTGCGATGGC TAATGGATGT GGTGGCATAT 
GCGCGAAACA AAAACGCTCA ACCCTCGTTG GCTATGCGAG AAATTCTGGA CTTTGCACAG 
CAAAGACAGG ACGAAGCGGT AGCGACATCG GCAGGAGGTT CCGCGAACAA ACAGCTGCTG 
CAGCTGCCCA TCCGCGAGAG CAAGTTCTCC AAGACGGGCC AGGGAAGAGG CAGTTGGCCG 
GGACCGGGAA CTAGTGAAGA TCAATCGCAG AACAAGCCGG AGCACTCCAA GTCTGAACAG 
AATCTGGAAC TGAGTGCTAT CACGCCAGTG GAACCTGCAT CCAATCAAGT TCCTACACAG 
CAGCAGCAGC CGCAGCAGCA ATCGCAGCAA CAGCAACTCT TACAAGTTTC CAACATCAGC 
GAATACGCGG GATCGATTTG CTCAGAAGTA TCATTTAGGA AGAATAGTGG CGATTCCATG 
AGCTCCACCT ACACGTCCCG GAGCTTCTAC TCTGCCGTGG ACTCAGCCTC AGATGGAAAT 
AGCACAAACA GTGTGTTTGC CATTCCGCCC TCTCGTTCCG ACGACACTCT GGCGGATGCA 
CTGCGCCACT CGCAAGCGGT AGCTGCGCAA CGCAAACGAA CCAGCTCTAA TATCGCATCT 
CATACCAATC CCTTGATTAC GGTGCACAGC TCTAGTTCGG CTCCGTACCT GGCTGGATCC 
AGTGTTTCCT TGAGAAGCGG CAACGGAGCC TTCGCAACGG ATTTGGAGCA CAAACCGCTG 
AAGCCAGCGA CAAAGGCTGC ATCTAGTGCA AACCTGCGCG AAGGTGGACC CTATTTGAAA 
AGCACGTTAG CTGAACTACG AGCTGTTGGA GGTGAGGAGC CGGTGGCCAG TACTTCAAAG 
GCCTCTTCGA TGAAGAGCTT GTCGCGGCAG AGCAGCGAAG AGGATTCCGC CAGCTGTTCC 
AGTCTCAACG CAGAGCAGAC ATCGGGGATC ATTCAAGTCT ATACCGCCTA CAGTACGGGT 
CTGGCCAGTG GAACCAGCTT AAAGATTCAC GTAACTCCGA AGACGACTGC CCGCGAAGTG 
ATCAACCTGG TGGTGAAGCA ACTTAACATG GCCGTGGTTC TCAAAGGGAA CAATGGCCCC 
ATCTATGGAC CCGAGATGCT AGAGAACTTT TGCCTGGTTG CGGTGATTGG AGCAAGGGAA 
CGCTGCCTGC GAGATGACTT CAAACCGCTG CAACTGCAGA ATCCCTGGAA GAAGGGCCGT 
CTGTACGTCC GCAAGAAGCA TGAGCTGCTG GCGGCCATCG AACACTCGAA TCGGAAATCG 
CATTTGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wake ( NM_001300540.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287469.1 Drosophila melanogaster wide awake (wake), transcript variant G, mRNA.

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CloneID	OFa22137
Clone ID Related Accession (Same CDS sequence)	NM_001300540.1
Accession Version	NM_001300540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wide awake, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCAGG ATGCCGCAGC AGGTCCTGCA AAGAAAAGCG GCACCACCCT ATCCGTCGAC 
CGGGCCGCCT TCCTTTTGCT GCGCGTCAGG AAAGTGTTCA AGAAGAAGCG ACAGCAGCGC 
AAGGAGCGAC AAGCCCAGCA AAGTGCCGCC GCAGCGGCGA GTGGTGCCGG CGGTGCGGGT 
GGTGCCGGTG GTGGTGGCGG TGGCTCCGGG TCACAATCGC GACCAGGCAG CCGGAAGCTG 
CCGCCGCCAT TGTTACGGTC GCGCACACTG CCCGCCATCA TTGTGCCAGG ACTTCCGGTC 
GTCTCGCTGC ACACGGATAA GCAAACATTT CAGCTGGACG AACGTTTGTT GGGCAGCAAA 
AGCCGCAGTG GCAGCGCCAG TGGCCATCGA TGGTCGCTGC TCACACGTGC CACCAACAGC 
AGCAGCATGT GCAGCAACAA CAACCCGGCC AGCAGTAGCA GCAGCAGCAG CAACAACAAT 
AACAATTTAC ACATGGCCAA CAACAATAAC ACACTGATGG TGAAATCATT GAATTTGCCC 
AAAGACGATA GCAATTTGGT CTATCGCCGC AAATCCTCGG GCAGCACGCC CCACCAACAT 
CAGAATGCGC ACCATCATCA TCAACAGCAT CATCACCTGC AGCAGCAGCA GCAACAGCAG 
CAGCAGCATC AGCAGCAGCA ACTGCAACAG CAGCAGATGC TGCAGCATCT GGGGGACAGC 
TCGCTGTTTC AGCTGTCCGA CGATTTCGAG TGCCACGCGG ATGGTTCCCT GCTCCTCAGA 
ATTCCAGCTC CTCATGTGGT CGCCGCACGA GCCTATCGAC TGGCAGCTCG AAAGCGAGCT 
GCCGCCTCCT CGGAGGCCTC CACACCGATG ACCCGTGAGC ACACGCTCCA CGAGATGCCG 
CTGGCGGAGT CGGCCTGCTC CGGATCTGCC ACTGGTTCGG GCACGACCTT CACGCGTCTG 
GCCAAATTGC TGCATCAATC AAGCACGCTG GCGGGCAGGT CCAATCTGCA GCCACCGCCC 
ATGGCTGACA ATGCGGTCCA GAGCCTGGGC AGAGGATTGG GCCTGGGACT GGGCGAGGAG 
GCGCCACGTC GTCTGTCCTG GGAGAGGCGC AAGGACAGCA GTGCTCTGCA GCGTTCGGCC 
AGCATTGATT CCTTTGCCGA GATTGTGTTT AGTGAGTCGC CACGCCCCTC GCTGGCTGTC 
ACGGGACCAG GTGCCTGCGC CTCCGCATCC GGCGGCCCTT CGCCATTCAG CAAACGTCCT 
TCGGCCAGCA GCCTGTACTC CACCTCAACG TCCAGCTCCT TCGCATCGCA GATGCAGGCC 
CAGCTGAATG TCAATTACGG CGACTCGGCA CTGGGATCCG GATCAGCAAC GGGTGGACCG 
GGCTCGGGTC ACCACCATGG GGCATCTGGT GGCGGCAACG GGGGCAGCAG TGCCAGCAGC 
AGTCGGCGCG AAAGCATGCT CAGCCCCTCA TCCACGCGCC GCTCCAAGCT GACCAGAATC 
ATAAACGCTC TCTTCTCGGC AGTGGAGCAT GGTCACCTGG AGAAGGCGCG CACTATTCTA 
GAGTCCACCG ACGTAGATGT AAACAGCATC AATAACGATG GACTATCTGC TCTGGACTTG 
GCGGTGTTAA GCAATAATCG CTCCATGACC AGGATGCTGC TTCAGCATGG CGCTGTGGAG 
GGCTCTCAGT TCTCTGTGGA CACCATTGGC ACCAAACTGA ACGGCTTGCT CAAGGACGCC 
GAGTCAAGAA TTCACGATCT AAGTGGACCG GAGGGCCTCT GTCCGCCGGT GTTTGCATCG 
CGTCCCTCCA TCTCGAGCAT CATAATTGGC AATTCCAGTG CATCGGTAAC TGGCTGCACG 
GGCAGCGAGG TGGAAAAACA GATAGGCATC TGGGAGCGCC GAGTTAAGGG ACTGCGTCGC 
TTGCAGCTCG GTTGGGATCA GGCCAGGCCG CCAGATGCAC CCGCCTCCGT GGTCGTCGAC 
GTCACTGGCG ACAATTCCAT CAGCGTACAA ATCCTAGAGC CCTTCGAGGG GGCCATCGGC 
ACGAAATTTA AAGTACAATG GTCGACCCGC GCGGATTTCA ACAACGTTGT GGGCGAGAGT 
GAGCTACTGG AATGGATCAG CTTCCATGGC ACGATGGGTG CCCAGTGTCA CATATCGGGC 
CTCACCCAGG GTCGTCGCTA CTTCCTGCGA GCCGCGTGCG GCAATGTGAA GGGATGGGGC 
ACCTATCGAA CCTCTGTGCC GGCTAGTGTA GTGCCCTCAA CTTGGCGGGA TTTAGACAAT 
CGAGAGGACC GATTCGTGGG TCGTCACCGA ATCCTGGACA ATCTATTTAC CGCCGTGCGT 
TTGGCAAGAC CCGCCGACGT ATCCGAGCTG ACTTTGGATC CTGCGAGCGC CCAACGACGT 
AATCCCAAAA AGAAGACTAC CATTAAGCAG TTGTTCTCGG TAACCACCAA GTTTCAAAAG 
ACACTGAGAC GCGGCATTTA CTTCTCTTGT ATTATTCATT GCGATGATAA AGTGCTGGTC 
ACCAGCGAAG ACTTTCCACC GGTCATCGAG GTGGATGAAT CCTATCCGAG TGCTTTGCAC 
ATGGATTACT ACTGGCTCAT GAAAGTTGCC TGCACCTGGG AGGATGTTAA GTCTCTACGT 
TCGGATATGG AGCGCAATCT TACCTCCGCT GTTCACTTCC GCACTAAGCT TCTATCGGCG 
GTCTGTCAGA TGCAATCGGC ACTAGGCATT ACGGACTTGG GCCAACTTTA CTATAAACCA 
TTGCGGGATG CTCAGGGCAC TGTGGTCCTA ACCTGTGTGC AGTCGGTAAA GAGCCAAAAG 
GCCGTCTCCA TCTTAAACTC GAGATGGGTT CCAGTCAGCA AGCTGCAAAA GAAGCTAGGT 
GCTCTACACG AGGACTACAC CATTAACGAG CTGCTCATCT CCTCGATTGG AGATCAATTG 
CACTATCAAC AGGCGGCGCT GCAGCGTTTG GAGCCCGGTC TTTACCTGGG CTACCTAAAG 
ATGCAATGCT CCATAGACCA GATCCAGGTG GTTGTGCCCG TGAAAACGCC GAATGTCTTG 
CCCCACTGCA AGGTGCGCGA AAACAGTCAC ATCACGGCCG AAGAGTGGCA GGTGCTCCAC 
CGTTACAGTA GCGATCCTCT ACGCCTGCCG TTGGACTTTA GCGCTCAAGG AGGAGATGGA 
GCCTCCGGAG CAACAGCTAC TACGGAGGTG CAACGCCTAT TTTTATACGA TCTCACCAAT 
GCAATGCACA AACTGTTTGC CAGTATGAAC ATCAAAATCG CTGATGCAAC CACTCACCGC 
TTGTACGACG TGGAGGTGAT TGAGCATAGT CCGGATATTA GTTTCCTCGT GGTGTGTCCA 
TCCGCCGAGT CCTCATGCGC TGTGCCCGGT CAGTCTGAGT TACTGCTACA GCGAGATGAC 
TTGGCCAGCT TGAGTATCCA GGCCTTTGAG ATGATTCACC TGCGCACTTA TCAGCCGGCC 
ATCATTCAGA AGTACGCCCG TTTATCTTGC ATCCTTGAAT TGGATACCGC ACTGGCCACA 
CATTCGCTTC GCGAGGCATT CTCCAGCAGT GAACTGCAGG CGGCCAAGGA ACGTTTGGCC 
ACGCTGCAGG AGCTCAGTGC GAGTCTTACG ATTGTGTGGA AAAGTGTGCG ATGGCTAATG 
GATGTGGTGG CATATGCGCG AAACAAAAAC GCTCAACCCT CGTTGGCTAT GCGAGAAATT 
CTGGACTTTG CACAGCAAAG ACAGGACGAA GCGGTAGCGA CATCGGCAGG AGGTTCCGCG 
AACAAACAGC TGCTGCAGCT GCCCATCCGC GAGAGCAAGT TCTCCAAGAC GGGCCAGGGA 
AGAGGCAGTT GGCCGGGACC GGGAACTAGT GAAGATCAAT CGCAGAACAA GCCGGAGCAC 
TCCAAGTCTG AACAGAATCT GGAACTGAGT GCTATCACGC CAGTGGAACC TGCATCCAAT 
CAAGTTCCTA CACAGCAGCA GCAGCCGCAG CAGCAATCGC AGCAACAGCA ACTCTTACAA 
GTTTCCAACA TCAGCGAATA CGCGGGATCG ATTTGCTCAG AAGTATCATT TAGGAAGAAT 
AGTGGCGATT CCATGAGCTC CACCTACACG TCCCGGAGCT TCTACTCTGC CGTGGACTCA 
GCCTCAGATG GAAATAGCAC AAACAGTGTG TTTGCCATTC CGCCCTCTCG TTCCGACGAC 
ACTCTGGCGG ATGCACTGCG CCACTCGCAA GCGGTAGCTG CGCAACGCAA ACGAACCAGC 
TCTAATATCG CATCTCATAC CAATCCCTTG ATTACGGTGC ACAGCTCTAG TTCGGCTCCG 
TACCTGGCTG GATCCAGTGT TTCCTTGAGA AGCGGCAACG GAGCCTTCGC AACGGATTTG 
GAGCACAAAC CGCTGAAGCC AGCGACAAAG GCTGCATCTA GTGCAAACCT GCGCGAAGGT 
GGACCCTATT TGAAAAGCAC GTTAGCTGAA CTACGAGCTG TTGGAGGTGA GGAGCCGGTG 
GCCAGTACTT CAAAGGCCTC TTCGATGAAG AGCTTGTCGC GGCAGAGCAG CGAAGAGGAT 
TCCGCCAGCT GTTCCAGTCT CAACGCAGAG CAGACATCGG GGATCATTCA AGTCTATACC 
GCCTACAGTA CGGGTCTGGC CAGTGGAACC AGCTTAAAGA TTCACGTAAC TCCGAAGACG 
ACTGCCCGCG AAGTGATCAA CCTGGTGGTG AAGCAACTTA ACATGGCCGT GGTTCTCAAA 
GGGAACAATG GCCCCATCTA TGGACCCGAG ATGCTAGAGA ACTTTTGCCT GGTTGCGGTG 
ATTGGAGCAA GGGAACGCTG CCTGCGAGAT GACTTCAAAC CGCTGCAACT GCAGAATCCC 
TGGAAGAAGG GCCGTCTGTA CGTCCGCAAG AAGCATGAGC TGCTGGCGGC CATCGAACAC 
TCGAATCGGA AATCGCATTT GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287469.1
CDS	374..5320
Translation	MEQDAAAGPAKKSGTTLSVDRAAFLLLRVRKVFKKKRQQRKERQAQQSAAAAASGAGGAGGAGGGGGGSGSQSRPGSRKLPPPLLRSRTLPAIIVPGLPVVSLHTDKQTFQLDERLLGSKSRSGSASGHRWSLLTRATNSSSMCSNNNPASSSSSSSNNNNNLHMANNNNTLMVKSLNLPKDDSNLVYRRKSSGSTPHQHQNAHHHHQQHHHLQQQQQQQQQHQQQQLQQQQMLQHLGDSSLFQLSDDFECHADGSLLLRIPAPHVVAARAYRLAARKRAAASSEASTPMTREHTLHEMPLAESACSGSATGSGTTFTRLAKLLHQSSTLAGRSNLQPPPMADNAVQSLGRGLGLGLGEEAPRRLSWERRKDSSALQRSASIDSFAEIVFSESPRPSLAVTGPGACASASGGPSPFSKRPSASSLYSTSTSSSFASQMQAQLNVNYGDSALGSGSATGGPGSGHHHGASGGGNGGSSASSSRRESMLSPSSTRRSKLTRIINALFSAVEHGHLEKARTILESTDVDVNSINNDGLSALDLAVLSNNRSMTRMLLQHGAVEGSQFSVDTIGTKLNGLLKDAESRIHDLSGPEGLCPPVFASRPSISSIIIGNSSASVTGCTGSEVEKQIGIWERRVKGLRRLQLGWDQARPPDAPASVVVDVTGDNSISVQILEPFEGAIGTKFKVQWSTRADFNNVVGESELLEWISFHGTMGAQCHISGLTQGRRYFLRAACGNVKGWGTYRTSVPASVVPSTWRDLDNREDRFVGRHRILDNLFTAVRLARPADVSELTLDPASAQRRNPKKKTTIKQLFSVTTKFQKTLRRGIYFSCIIHCDDKVLVTSEDFPPVIEVDESYPSALHMDYYWLMKVACTWEDVKSLRSDMERNLTSAVHFRTKLLSAVCQMQSALGITDLGQLYYKPLRDAQGTVVLTCVQSVKSQKAVSILNSRWVPVSKLQKKLGALHEDYTINELLISSIGDQLHYQQAALQRLEPGLYLGYLKMQCSIDQIQVVVPVKTPNVLPHCKVRENSHITAEEWQVLHRYSSDPLRLPLDFSAQGGDGASGATATTEVQRLFLYDLTNAMHKLFASMNIKIADATTHRLYDVEVIEHSPDISFLVVCPSAESSCAVPGQSELLLQRDDLASLSIQAFEMIHLRTYQPAIIQKYARLSCILELDTALATHSLREAFSSSELQAAKERLATLQELSASLTIVWKSVRWLMDVVAYARNKNAQPSLAMREILDFAQQRQDEAVATSAGGSANKQLLQLPIRESKFSKTGQGRGSWPGPGTSEDQSQNKPEHSKSEQNLELSAITPVEPASNQVPTQQQQPQQQSQQQQLLQVSNISEYAGSICSEVSFRKNSGDSMSSTYTSRSFYSAVDSASDGNSTNSVFAIPPSRSDDTLADALRHSQAVAAQRKRTSSNIASHTNPLITVHSSSSAPYLAGSSVSLRSGNGAFATDLEHKPLKPATKAASSANLREGGPYLKSTLAELRAVGGEEPVASTSKASSMKSLSRQSSEEDSASCSSLNAEQTSGIIQVYTAYSTGLASGTSLKIHVTPKTTAREVINLVVKQLNMAVVLKGNNGPIYGPEMLENFCLVAVIGARERCLRDDFKPLQLQNPWKKGRLYVRKKHELLAAIEHSNRKSHLI

Target ORF information:

RefSeq Version	NM_001300540.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wide awake (wake), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300540.1

ORF Insert Sequence:

ATGGAGCAGG ATGCCGCAGC AGGTCCTGCA AAGAAAAGCG GCACCACCCT ATCCGTCGAC 
CGGGCCGCCT TCCTTTTGCT GCGCGTCAGG AAAGTGTTCA AGAAGAAGCG ACAGCAGCGC 
AAGGAGCGAC AAGCCCAGCA AAGTGCCGCC GCAGCGGCGA GTGGTGCCGG CGGTGCGGGT 
GGTGCCGGTG GTGGTGGCGG TGGCTCCGGG TCACAATCGC GACCAGGCAG CCGGAAGCTG 
CCGCCGCCAT TGTTACGGTC GCGCACACTG CCCGCCATCA TTGTGCCAGG ACTTCCGGTC 
GTCTCGCTGC ACACGGATAA GCAAACATTT CAGCTGGACG AACGTTTGTT GGGCAGCAAA 
AGCCGCAGTG GCAGCGCCAG TGGCCATCGA TGGTCGCTGC TCACACGTGC CACCAACAGC 
AGCAGCATGT GCAGCAACAA CAACCCGGCC AGCAGTAGCA GCAGCAGCAG CAACAACAAT 
AACAATTTAC ACATGGCCAA CAACAATAAC ACACTGATGG TGAAATCATT GAATTTGCCC 
AAAGACGATA GCAATTTGGT CTATCGCCGC AAATCCTCGG GCAGCACGCC CCACCAACAT 
CAGAATGCGC ACCATCATCA TCAACAGCAT CATCACCTGC AGCAGCAGCA GCAACAGCAG 
CAGCAGCATC AGCAGCAGCA ACTGCAACAG CAGCAGATGC TGCAGCATCT GGGGGACAGC 
TCGCTGTTTC AGCTGTCCGA CGATTTCGAG TGCCACGCGG ATGGTTCCCT GCTCCTCAGA 
ATTCCAGCTC CTCATGTGGT CGCCGCACGA GCCTATCGAC TGGCAGCTCG AAAGCGAGCT 
GCCGCCTCCT CGGAGGCCTC CACACCGATG ACCCGTGAGC ACACGCTCCA CGAGATGCCG 
CTGGCGGAGT CGGCCTGCTC CGGATCTGCC ACTGGTTCGG GCACGACCTT CACGCGTCTG 
GCCAAATTGC TGCATCAATC AAGCACGCTG GCGGGCAGGT CCAATCTGCA GCCACCGCCC 
ATGGCTGACA ATGCGGTCCA GAGCCTGGGC AGAGGATTGG GCCTGGGACT GGGCGAGGAG 
GCGCCACGTC GTCTGTCCTG GGAGAGGCGC AAGGACAGCA GTGCTCTGCA GCGTTCGGCC 
AGCATTGATT CCTTTGCCGA GATTGTGTTT AGTGAGTCGC CACGCCCCTC GCTGGCTGTC 
ACGGGACCAG GTGCCTGCGC CTCCGCATCC GGCGGCCCTT CGCCATTCAG CAAACGTCCT 
TCGGCCAGCA GCCTGTACTC CACCTCAACG TCCAGCTCCT TCGCATCGCA GATGCAGGCC 
CAGCTGAATG TCAATTACGG CGACTCGGCA CTGGGATCCG GATCAGCAAC GGGTGGACCG 
GGCTCGGGTC ACCACCATGG GGCATCTGGT GGCGGCAACG GGGGCAGCAG TGCCAGCAGC 
AGTCGGCGCG AAAGCATGCT CAGCCCCTCA TCCACGCGCC GCTCCAAGCT GACCAGAATC 
ATAAACGCTC TCTTCTCGGC AGTGGAGCAT GGTCACCTGG AGAAGGCGCG CACTATTCTA 
GAGTCCACCG ACGTAGATGT AAACAGCATC AATAACGATG GACTATCTGC TCTGGACTTG 
GCGGTGTTAA GCAATAATCG CTCCATGACC AGGATGCTGC TTCAGCATGG CGCTGTGGAG 
GGCTCTCAGT TCTCTGTGGA CACCATTGGC ACCAAACTGA ACGGCTTGCT CAAGGACGCC 
GAGTCAAGAA TTCACGATCT AAGTGGACCG GAGGGCCTCT GTCCGCCGGT GTTTGCATCG 
CGTCCCTCCA TCTCGAGCAT CATAATTGGC AATTCCAGTG CATCGGTAAC TGGCTGCACG 
GGCAGCGAGG TGGAAAAACA GATAGGCATC TGGGAGCGCC GAGTTAAGGG ACTGCGTCGC 
TTGCAGCTCG GTTGGGATCA GGCCAGGCCG CCAGATGCAC CCGCCTCCGT GGTCGTCGAC 
GTCACTGGCG ACAATTCCAT CAGCGTACAA ATCCTAGAGC CCTTCGAGGG GGCCATCGGC 
ACGAAATTTA AAGTACAATG GTCGACCCGC GCGGATTTCA ACAACGTTGT GGGCGAGAGT 
GAGCTACTGG AATGGATCAG CTTCCATGGC ACGATGGGTG CCCAGTGTCA CATATCGGGC 
CTCACCCAGG GTCGTCGCTA CTTCCTGCGA GCCGCGTGCG GCAATGTGAA GGGATGGGGC 
ACCTATCGAA CCTCTGTGCC GGCTAGTGTA GTGCCCTCAA CTTGGCGGGA TTTAGACAAT 
CGAGAGGACC GATTCGTGGG TCGTCACCGA ATCCTGGACA ATCTATTTAC CGCCGTGCGT 
TTGGCAAGAC CCGCCGACGT ATCCGAGCTG ACTTTGGATC CTGCGAGCGC CCAACGACGT 
AATCCCAAAA AGAAGACTAC CATTAAGCAG TTGTTCTCGG TAACCACCAA GTTTCAAAAG 
ACACTGAGAC GCGGCATTTA CTTCTCTTGT ATTATTCATT GCGATGATAA AGTGCTGGTC 
ACCAGCGAAG ACTTTCCACC GGTCATCGAG GTGGATGAAT CCTATCCGAG TGCTTTGCAC 
ATGGATTACT ACTGGCTCAT GAAAGTTGCC TGCACCTGGG AGGATGTTAA GTCTCTACGT 
TCGGATATGG AGCGCAATCT TACCTCCGCT GTTCACTTCC GCACTAAGCT TCTATCGGCG 
GTCTGTCAGA TGCAATCGGC ACTAGGCATT ACGGACTTGG GCCAACTTTA CTATAAACCA 
TTGCGGGATG CTCAGGGCAC TGTGGTCCTA ACCTGTGTGC AGTCGGTAAA GAGCCAAAAG 
GCCGTCTCCA TCTTAAACTC GAGATGGGTT CCAGTCAGCA AGCTGCAAAA GAAGCTAGGT 
GCTCTACACG AGGACTACAC CATTAACGAG CTGCTCATCT CCTCGATTGG AGATCAATTG 
CACTATCAAC AGGCGGCGCT GCAGCGTTTG GAGCCCGGTC TTTACCTGGG CTACCTAAAG 
ATGCAATGCT CCATAGACCA GATCCAGGTG GTTGTGCCCG TGAAAACGCC GAATGTCTTG 
CCCCACTGCA AGGTGCGCGA AAACAGTCAC ATCACGGCCG AAGAGTGGCA GGTGCTCCAC 
CGTTACAGTA GCGATCCTCT ACGCCTGCCG TTGGACTTTA GCGCTCAAGG AGGAGATGGA 
GCCTCCGGAG CAACAGCTAC TACGGAGGTG CAACGCCTAT TTTTATACGA TCTCACCAAT 
GCAATGCACA AACTGTTTGC CAGTATGAAC ATCAAAATCG CTGATGCAAC CACTCACCGC 
TTGTACGACG TGGAGGTGAT TGAGCATAGT CCGGATATTA GTTTCCTCGT GGTGTGTCCA 
TCCGCCGAGT CCTCATGCGC TGTGCCCGGT CAGTCTGAGT TACTGCTACA GCGAGATGAC 
TTGGCCAGCT TGAGTATCCA GGCCTTTGAG ATGATTCACC TGCGCACTTA TCAGCCGGCC 
ATCATTCAGA AGTACGCCCG TTTATCTTGC ATCCTTGAAT TGGATACCGC ACTGGCCACA 
CATTCGCTTC GCGAGGCATT CTCCAGCAGT GAACTGCAGG CGGCCAAGGA ACGTTTGGCC 
ACGCTGCAGG AGCTCAGTGC GAGTCTTACG ATTGTGTGGA AAAGTGTGCG ATGGCTAATG 
GATGTGGTGG CATATGCGCG AAACAAAAAC GCTCAACCCT CGTTGGCTAT GCGAGAAATT 
CTGGACTTTG CACAGCAAAG ACAGGACGAA GCGGTAGCGA CATCGGCAGG AGGTTCCGCG 
AACAAACAGC TGCTGCAGCT GCCCATCCGC GAGAGCAAGT TCTCCAAGAC GGGCCAGGGA 
AGAGGCAGTT GGCCGGGACC GGGAACTAGT GAAGATCAAT CGCAGAACAA GCCGGAGCAC 
TCCAAGTCTG AACAGAATCT GGAACTGAGT GCTATCACGC CAGTGGAACC TGCATCCAAT 
CAAGTTCCTA CACAGCAGCA GCAGCCGCAG CAGCAATCGC AGCAACAGCA ACTCTTACAA 
GTTTCCAACA TCAGCGAATA CGCGGGATCG ATTTGCTCAG AAGTATCATT TAGGAAGAAT 
AGTGGCGATT CCATGAGCTC CACCTACACG TCCCGGAGCT TCTACTCTGC CGTGGACTCA 
GCCTCAGATG GAAATAGCAC AAACAGTGTG TTTGCCATTC CGCCCTCTCG TTCCGACGAC 
ACTCTGGCGG ATGCACTGCG CCACTCGCAA GCGGTAGCTG CGCAACGCAA ACGAACCAGC 
TCTAATATCG CATCTCATAC CAATCCCTTG ATTACGGTGC ACAGCTCTAG TTCGGCTCCG 
TACCTGGCTG GATCCAGTGT TTCCTTGAGA AGCGGCAACG GAGCCTTCGC AACGGATTTG 
GAGCACAAAC CGCTGAAGCC AGCGACAAAG GCTGCATCTA GTGCAAACCT GCGCGAAGGT 
GGACCCTATT TGAAAAGCAC GTTAGCTGAA CTACGAGCTG TTGGAGGTGA GGAGCCGGTG 
GCCAGTACTT CAAAGGCCTC TTCGATGAAG AGCTTGTCGC GGCAGAGCAG CGAAGAGGAT 
TCCGCCAGCT GTTCCAGTCT CAACGCAGAG CAGACATCGG GGATCATTCA AGTCTATACC 
GCCTACAGTA CGGGTCTGGC CAGTGGAACC AGCTTAAAGA TTCACGTAAC TCCGAAGACG 
ACTGCCCGCG AAGTGATCAA CCTGGTGGTG AAGCAACTTA ACATGGCCGT GGTTCTCAAA 
GGGAACAATG GCCCCATCTA TGGACCCGAG ATGCTAGAGA ACTTTTGCCT GGTTGCGGTG 
ATTGGAGCAA GGGAACGCTG CCTGCGAGAT GACTTCAAAC CGCTGCAACT GCAGAATCCC 
TGGAAGAAGG GCCGTCTGTA CGTCCGCAAG AAGCATGAGC TGCTGGCGGC CATCGAACAC 
TCGAATCGGA AATCGCATTT GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wake cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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