spn-E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spn-E
Entrez Gene ID	41919
Full Name	spindle E
Synonyms	CG3158,Dmel\CG3158,SPN-E,Spn-E,SpnE,fs(1)hls,fs(3)hls,hes,hls,homeless,spin-E,spn E,spn-E/hls,spnE
General protein information	Preferred Names spindle E Names CG3158 gene product from transcript CG3158-RA CG3158-PA Spindle-E (homeless) SpindleE female sterile(3)homeless homeless homeless/spindle-E spindle-E spn-E-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89A5-89A5\|3-62 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057393.4	NP_476741.1	spindle E

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TDRD9	XP_510193.3
	TDRD9	XP_001089930.2
Bos taurus (cattle)	TDRD9	XP_002696831.3
Mus musculus (house mouse)	Tdrd9	NP_083332.1
Rattus norvegicus (Norway rat)	Tdrd9	XP_001078318.3
Homo sapiens (human)	TDRD9	NP_694591.2
Drosophila melanogaster (fruit fly)	spn-E	NP_476741.1
Anopheles gambiae (African malaria mosquito)	SPNE_ANOGA	XP_312084.5
Gallus gallus (chicken)	TDRD9	XP_004936444.1
Xenopus tropicalis (tropical clawed frog)	tdrd9	NP_001238746.1
Canis lupus familiaris (dog)	TDRD9	XP_005623997.1
Danio rerio (zebrafish)	tdrd9	NP_001123984.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The lack of Spn-E caused a significant drop of protein levels of key ping-pong participants, Aubergine (Aub) and AGO3 proteins of PIWI subfamily, in the germline of both males and females, but did not disrupt of their assembly in nuage granules.
Title: RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila.

DExH box domain of Spindle-E is necessary for its function in the piRNA pathway and retrotransposon silencing during oogenesis and embryonic patterning.
Title: The DExH box helicase domain of spindle-E is necessary for retrotransposon silencing and axial patterning during Drosophila oogenesis.

mutations in piwi, aubergine, or spindle-E which encode RNAi components result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2
Title: Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery.

RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E.
Title: RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E.

The following spn-E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spn-E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05043	NM_057393.4 Latest version!	Drosophila melanogaster spindle E (spn-E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spn-E ( NM_057393.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476741.1 Drosophila melanogaster spindle E (spn-E), mRNA.

Price & Availability↑

CloneID	OFa05043
Clone ID Related Accession (Same CDS sequence)	NM_057393.4
Accession Version	NM_057393.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spindle E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057393.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGG AGGTTATGGA TTTCTTTGAC TTCTCGAAGG AGTTGAAGCG CGTGGCCGCC 
GCCCCGCAAG GCTACATCAG CAGCGATCCG AGGCTAATGG CCACGAAGTT CAAATCATCC 
GAAGTGCCCA ACCGCGAGTT GATCGGCACC GACTATGTCA GCAAAATCGT CGCCAAGGAA 
AAGTGTCTCC TGAACGGGAC GCTGCTCAAC GAACAGCCTC AAGGCAAACG CATTCGAACG 
CTGGACGATT TGGACACCGA CGATGAGGGG GAAGAGACCG AAATTCGCCG GGATGACGAG 
TACTACAAGA AGTTCAGATT CAATCTGAAT CGCGACAAGA ACTTGTCCAT CTACGCCAAG 
CGCGAGGAAA TATTGGCGGC CATCAATGCT CATCCGGTAG TCATTATTAA GGGCGAAACA 
GGATGTGGCA AGACCACGCA GGTGCCCCAA TACATTTTGG ATGAGGCCTA TAAAAGCGGT 
AAATACTGCA ACATTGTGGT GACACAACCC CGGCGCATAG CTGCCATCTC CATAGCGAAT 
AGAGTTTGCC AGGAGCGCGA GTGGCAGCAG AACACGGTGT GCAGCTTCCA GGTGGGCCTG 
CACCGCCCGA ACTCTTTGGA GGACACTCGT CTGTTGTACT GCACCACCGG GGTGCTGCTG 
AACAATTTGA TTAACAACAA GACGTTGACC CACTACACCC ACATTGTGCT GGACGAGGTG 
CATGAAAGAG ACCAGAACAT GGATTTTCTT CTGATCGTGG TGCGTCGCTT GCTGGCCACG 
AACTCGCGGC ATGTAAAGAT TATCCTGATG TCGGCCACCA TCGATGCCAA GGAACTGTCC 
GACTACTTTA CCACCACTAA CTCAATTCCG CCAGTGATCA CCACCAATCA TCGACGTAAG 
CACTCGATCG AGAAGTTCTA CCGTGACCAG CTCGGCAGCA TTATTTGGAA TGAGGAAGAT 
GTGGGACACC AGCAGGTGCC AGAAATCAAC AAACACGGTT ACAGGGCAGC GGTAAAGATC 
ATAGTGATTA TTGACAACAT GGAACGGAAG GCTGCAATCC AGTCGCGGCA GAGCTATGAC 
GAGGCCCTGC GCTATGGAGC TGTGCTCATC TTTCTGCCAG GCATCTACGA AATCGACACG 
ATGGCAGAAA ATCTTACTTG TATGCTGGAA AACGACCCCA ACATTAAGGT CTCTATTGTT 
CGCTGTTTTT CGTTAATGAC ACCGGAAAAC CAACGGGATG TCTTCAATCC CCCGCCTCCC 
GGTTTCCGCA AGATCATTCT GACCACAAAC ATTGCTGAGA GCTCCATCAC GGTGCCAGAT 
GTCTCATACG TTATTGACTT TTGTCTTGCC AAAGTAAAGG TAACCGATAC GGCTAGCAGC 
TTCTCTTCGC TCCGCTTAAC CTGGGCGTCC AAAGCCAATT GTCGTCAGCG TGCCGGCCGC 
GTTGGTCGTC TTCGTAGTGG ACGCGTTTAC CGTATGGTTA ATAAACACTT CTATCAGAGG 
GAAATGCCCG AGTTTGGAAT ACCAGAAATG TTGCGCTTGC CCTTGCAAAA CTCGGTGCTC 
AAGGCCAAAG TGCTGAATAT GGGATCACCG GTGGAAATCT TGGCATTGGC TCTATCTCCG 
CCGAATCTGT CGGACATACA CAATACAATA TTGCTTCTGA AAGAGGTGGG TGCTCTGTAC 
TTAACAGTAG ACGGCATCTA CGACCCGTTG GATGGCGATC TTACCTACTG GGGCACAATC 
ATGTCTCGGC TGCCGTTGGA CACACGTCAG TCCCGGCTTA TCATATTGGG TTATATTTTT 
AACATGCTCG AAGAAGCTAT TATTATAGCT GCTGGCTTAT CGACGCCTGG CTTGTTTGCG 
CACGAAGGTG GTAGGTCTCA ATTAGGCGAT TCATTTTGGA TGCACTATAT CTTCTCCGAT 
GGATCTGGCT CCGACTTGGT GGCCATTTGG CGCGTGTATC TGACATATTT GAACATTGTG 
GAAAATGGTC ACGACCAGGA GTCTGCTATC CGTTGGGCCA AACGTTTCCA CGTCTCCTTG 
CGGTCACTTA AGGAGATACA CTTGTTGGTG CAGGAACTGC GTGTGCGATG CACGCATCTC 
GGTCTGATTC CGTTTCCTGT TAATCCTAAC CAAATGATGG ACGATCGTGA AAAAGCGATC 
ATGCTCAAAG TGATCATTGC AGGTGCCTTC TACCCAAACT ACTTTACGCG TTCCAAAGAG 
TCGTGTGCAG ACACAGATCG CAATATATAC CAGACAATAT CGGGCCACGA TCCCTGCCGC 
ACCGTATATT TTACCAATTT TAAGCCTGCC TACATGGGCG AGTTGTACAC TAGGCGCATT 
AAGGAGCTAT TTCAGGAGGT CAGGATTCCG CCGGAAAACA TGGACGTTAC TTTTCAAGAG 
GGTTCGCAAA AGGTCTTTGT CACTTTCAAA CAGGATGACT GGATTGAAGG TTCATCTAAG 
TATGTTCCTG TGTCGGGCAG AGTTCAAAGT GAGGTGTACA AGGCAGTTAT GATGCGACAA 
AATCGTGTCG AACGGCCAAT CCACATAATG AATCCGAGTG CATTTATGAG CTACGTACAG 
CAGCGAGGGA TTGGAGATGT GATTGAGGGC CGTTGGATTC CACCCACAAA GCCTTTAAAC 
GTCGAGCTTC TTGCGCTGCC ATCTGTCTTT GACAAAACTA TTTCAGGGTC GATTACTTGC 
ATCGTCAACT GTGGCAAGTT CTTTTTTCAA CCCCAATCTT TTGAAGAGTG CATTCGAAAT 
ATGTCTGAAA TTTTCAATGC TCCACAGCAA CTAAGAAATT ACGTAACTAA CGCGAGTGCC 
ATCGCAAAGG GCATGATGGT GTTGGCCAAA CGCGATAGTT ATTTCCAGCG CGCCACAGTG 
ATCCGGCCAG AGAATCAGAG CAATCGCCAA CCAATGTTCT ACGTTCGCTT CATCGACTAT 
GGTAATTGCA CTTTGCTGCC CATGCAGCTA ATGCGTTTGA TGCCGAGAGA GCTAACTGAA 
CAGTACGGCG ACCTTCCGCC ACGTGTTTTT GAGTGCAGGC TGGCAATGGT TCAGCCCTCT 
TCAGTGGTCA GTGGAAACAA TAGGTGGTCC ACCGCGGCTA ATGATATGCT CAAGACAGTG 
GCCCAATGCG GTCTAATCGA TATTGAGGTG TATTCGCTTT TCAATAATGT AGCAGCTGTT 
CTCATTCATA TGAGAGATGG CATAATCAAC GATAAGCTAG TAGAGCTTAT GTTGTGCCGT 
CGATCCGACG AAGATTATAT GTCTAGAAAA GATCATGATT TCCGTTTGCG ACGTCAAGAA 
TCTGCCCGGA ACTTGAGCAC GGCGCAGCGT CAACAGATCA ATGAGGAATA TCTGCGATCC 
TGTCAGCTGC CGCAAGACCA CGATCTACCA CCGCCTCCGC TGGAAAAGTG CAAGACCGTT 
GTAATGTTGA AAGGACCCAA CAGTCCTTTG GAATGTACTA TGCGATCCAT TACTCGTGTG 
GGCTTAAGTA AACGGGTGAA TATAGACCAT TTGTCCGTTA ATGCACTTCT CCTGGATGCT 
GATCCTCAAG ATCACCACGA TCATTTGATA GTGGCCCACG AAATCGCGGA GAGTAGAAAC 
GGGCAGACAC TGACAGCCCG TGGAACCACC CTGATGCCCA ATGTGCAGGG ATTCGGTGCT 
CTAATGGTTA TGCTATTTAG TCCCACTATG CAACTTAAAT GCAATAAAGA AGGCACATCA 
TATGTCTCTG TTCTGGGAGG ATTGGGTTGT GACCCGGACA CAAACGAGCC CTACTTCGCA 
GAGCATGACG TGTTGATTAA TCTGGATGTC AACATTCTCG AAGATGATGT GATTTTGATT 
AATCAAATTC GCTACTATAT AGATAGTGTC TTCTTCAACT TTAAGGAAGA AAACAATCCA 
GCTGTTAGCG TAAATGAACG TGTTTCTATA TACACTCAGC TGCGTAGCCT TATAAATAGG 
CTCCTTTGCA AGGATCGTAG ATATATCGAA AGAAATATGA GCAATGCGGA TTTTGAATGG 
GAAACCAATC CCGAACTACC ACTGCCGAAT GAACCTTTTG GCAAGAGGGC CATATTTCCG 
ATGCACTCAC TTACTGAGCT CCAAGAGGAA GACACGGGTC GCTTGGTGCA ACTGAGAGAG 
AACTGCAGCA TGTTGCACAA ATGGCGCAAC TTCGAAGGCA CTTTACCCCA TATGACTTGC 
AAACTGTGCA ATCAGTTGTT GGAATCGGTT CCCCAGCTCC GTTTGCATCT CCTGACCATA 
TTGCATCGTG ATCGTGAAAA GCAAATCGAC TATTGCAACC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476741.1
CDS	65..4369
Translation	MDQEVMDFFDFSKELKRVAAAPQGYISSDPRLMATKFKSSEVPNRELIGTDYVSKIVAKEKCLLNGTLLNEQPQGKRIRTLDDLDTDDEGEETEIRRDDEYYKKFRFNLNRDKNLSIYAKREEILAAINAHPVVIIKGETGCGKTTQVPQYILDEAYKSGKYCNIVVTQPRRIAAISIANRVCQEREWQQNTVCSFQVGLHRPNSLEDTRLLYCTTGVLLNNLINNKTLTHYTHIVLDEVHERDQNMDFLLIVVRRLLATNSRHVKIILMSATIDAKELSDYFTTTNSIPPVITTNHRRKHSIEKFYRDQLGSIIWNEEDVGHQQVPEINKHGYRAAVKIIVIIDNMERKAAIQSRQSYDEALRYGAVLIFLPGIYEIDTMAENLTCMLENDPNIKVSIVRCFSLMTPENQRDVFNPPPPGFRKIILTTNIAESSITVPDVSYVIDFCLAKVKVTDTASSFSSLRLTWASKANCRQRAGRVGRLRSGRVYRMVNKHFYQREMPEFGIPEMLRLPLQNSVLKAKVLNMGSPVEILALALSPPNLSDIHNTILLLKEVGALYLTVDGIYDPLDGDLTYWGTIMSRLPLDTRQSRLIILGYIFNMLEEAIIIAAGLSTPGLFAHEGGRSQLGDSFWMHYIFSDGSGSDLVAIWRVYLTYLNIVENGHDQESAIRWAKRFHVSLRSLKEIHLLVQELRVRCTHLGLIPFPVNPNQMMDDREKAIMLKVIIAGAFYPNYFTRSKESCADTDRNIYQTISGHDPCRTVYFTNFKPAYMGELYTRRIKELFQEVRIPPENMDVTFQEGSQKVFVTFKQDDWIEGSSKYVPVSGRVQSEVYKAVMMRQNRVERPIHIMNPSAFMSYVQQRGIGDVIEGRWIPPTKPLNVELLALPSVFDKTISGSITCIVNCGKFFFQPQSFEECIRNMSEIFNAPQQLRNYVTNASAIAKGMMVLAKRDSYFQRATVIRPENQSNRQPMFYVRFIDYGNCTLLPMQLMRLMPRELTEQYGDLPPRVFECRLAMVQPSSVVSGNNRWSTAANDMLKTVAQCGLIDIEVYSLFNNVAAVLIHMRDGIINDKLVELMLCRRSDEDYMSRKDHDFRLRRQESARNLSTAQRQQINEEYLRSCQLPQDHDLPPPPLEKCKTVVMLKGPNSPLECTMRSITRVGLSKRVNIDHLSVNALLLDADPQDHHDHLIVAHEIAESRNGQTLTARGTTLMPNVQGFGALMVMLFSPTMQLKCNKEGTSYVSVLGGLGCDPDTNEPYFAEHDVLINLDVNILEDDVILINQIRYYIDSVFFNFKEENNPAVSVNERVSIYTQLRSLINRLLCKDRRYIERNMSNADFEWETNPELPLPNEPFGKRAIFPMHSLTELQEEDTGRLVQLRENCSMLHKWRNFEGTLPHMTCKLCNQLLESVPQLRLHLLTILHRDREKQIDYCNQ

Target ORF information:

RefSeq Version	NM_057393.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spindle E (spn-E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057393.4

ORF Insert Sequence:

ATGGATCAGG AGGTTATGGA TTTCTTTGAC TTCTCGAAGG AGTTGAAGCG CGTGGCCGCC 
GCCCCGCAAG GCTACATCAG CAGCGATCCG AGGCTAATGG CCACGAAGTT CAAATCATCC 
GAAGTGCCCA ACCGCGAGTT GATCGGCACC GACTATGTCA GCAAAATCGT CGCCAAGGAA 
AAGTGTCTCC TGAACGGGAC GCTGCTCAAC GAACAGCCTC AAGGCAAACG CATTCGAACG 
CTGGACGATT TGGACACCGA CGATGAGGGG GAAGAGACCG AAATTCGCCG GGATGACGAG 
TACTACAAGA AGTTCAGATT CAATCTGAAT CGCGACAAGA ACTTGTCCAT CTACGCCAAG 
CGCGAGGAAA TATTGGCGGC CATCAATGCT CATCCGGTAG TCATTATTAA GGGCGAAACA 
GGATGTGGCA AGACCACGCA GGTGCCCCAA TACATTTTGG ATGAGGCCTA TAAAAGCGGT 
AAATACTGCA ACATTGTGGT GACACAACCC CGGCGCATAG CTGCCATCTC CATAGCGAAT 
AGAGTTTGCC AGGAGCGCGA GTGGCAGCAG AACACGGTGT GCAGCTTCCA GGTGGGCCTG 
CACCGCCCGA ACTCTTTGGA GGACACTCGT CTGTTGTACT GCACCACCGG GGTGCTGCTG 
AACAATTTGA TTAACAACAA GACGTTGACC CACTACACCC ACATTGTGCT GGACGAGGTG 
CATGAAAGAG ACCAGAACAT GGATTTTCTT CTGATCGTGG TGCGTCGCTT GCTGGCCACG 
AACTCGCGGC ATGTAAAGAT TATCCTGATG TCGGCCACCA TCGATGCCAA GGAACTGTCC 
GACTACTTTA CCACCACTAA CTCAATTCCG CCAGTGATCA CCACCAATCA TCGACGTAAG 
CACTCGATCG AGAAGTTCTA CCGTGACCAG CTCGGCAGCA TTATTTGGAA TGAGGAAGAT 
GTGGGACACC AGCAGGTGCC AGAAATCAAC AAACACGGTT ACAGGGCAGC GGTAAAGATC 
ATAGTGATTA TTGACAACAT GGAACGGAAG GCTGCAATCC AGTCGCGGCA GAGCTATGAC 
GAGGCCCTGC GCTATGGAGC TGTGCTCATC TTTCTGCCAG GCATCTACGA AATCGACACG 
ATGGCAGAAA ATCTTACTTG TATGCTGGAA AACGACCCCA ACATTAAGGT CTCTATTGTT 
CGCTGTTTTT CGTTAATGAC ACCGGAAAAC CAACGGGATG TCTTCAATCC CCCGCCTCCC 
GGTTTCCGCA AGATCATTCT GACCACAAAC ATTGCTGAGA GCTCCATCAC GGTGCCAGAT 
GTCTCATACG TTATTGACTT TTGTCTTGCC AAAGTAAAGG TAACCGATAC GGCTAGCAGC 
TTCTCTTCGC TCCGCTTAAC CTGGGCGTCC AAAGCCAATT GTCGTCAGCG TGCCGGCCGC 
GTTGGTCGTC TTCGTAGTGG ACGCGTTTAC CGTATGGTTA ATAAACACTT CTATCAGAGG 
GAAATGCCCG AGTTTGGAAT ACCAGAAATG TTGCGCTTGC CCTTGCAAAA CTCGGTGCTC 
AAGGCCAAAG TGCTGAATAT GGGATCACCG GTGGAAATCT TGGCATTGGC TCTATCTCCG 
CCGAATCTGT CGGACATACA CAATACAATA TTGCTTCTGA AAGAGGTGGG TGCTCTGTAC 
TTAACAGTAG ACGGCATCTA CGACCCGTTG GATGGCGATC TTACCTACTG GGGCACAATC 
ATGTCTCGGC TGCCGTTGGA CACACGTCAG TCCCGGCTTA TCATATTGGG TTATATTTTT 
AACATGCTCG AAGAAGCTAT TATTATAGCT GCTGGCTTAT CGACGCCTGG CTTGTTTGCG 
CACGAAGGTG GTAGGTCTCA ATTAGGCGAT TCATTTTGGA TGCACTATAT CTTCTCCGAT 
GGATCTGGCT CCGACTTGGT GGCCATTTGG CGCGTGTATC TGACATATTT GAACATTGTG 
GAAAATGGTC ACGACCAGGA GTCTGCTATC CGTTGGGCCA AACGTTTCCA CGTCTCCTTG 
CGGTCACTTA AGGAGATACA CTTGTTGGTG CAGGAACTGC GTGTGCGATG CACGCATCTC 
GGTCTGATTC CGTTTCCTGT TAATCCTAAC CAAATGATGG ACGATCGTGA AAAAGCGATC 
ATGCTCAAAG TGATCATTGC AGGTGCCTTC TACCCAAACT ACTTTACGCG TTCCAAAGAG 
TCGTGTGCAG ACACAGATCG CAATATATAC CAGACAATAT CGGGCCACGA TCCCTGCCGC 
ACCGTATATT TTACCAATTT TAAGCCTGCC TACATGGGCG AGTTGTACAC TAGGCGCATT 
AAGGAGCTAT TTCAGGAGGT CAGGATTCCG CCGGAAAACA TGGACGTTAC TTTTCAAGAG 
GGTTCGCAAA AGGTCTTTGT CACTTTCAAA CAGGATGACT GGATTGAAGG TTCATCTAAG 
TATGTTCCTG TGTCGGGCAG AGTTCAAAGT GAGGTGTACA AGGCAGTTAT GATGCGACAA 
AATCGTGTCG AACGGCCAAT CCACATAATG AATCCGAGTG CATTTATGAG CTACGTACAG 
CAGCGAGGGA TTGGAGATGT GATTGAGGGC CGTTGGATTC CACCCACAAA GCCTTTAAAC 
GTCGAGCTTC TTGCGCTGCC ATCTGTCTTT GACAAAACTA TTTCAGGGTC GATTACTTGC 
ATCGTCAACT GTGGCAAGTT CTTTTTTCAA CCCCAATCTT TTGAAGAGTG CATTCGAAAT 
ATGTCTGAAA TTTTCAATGC TCCACAGCAA CTAAGAAATT ACGTAACTAA CGCGAGTGCC 
ATCGCAAAGG GCATGATGGT GTTGGCCAAA CGCGATAGTT ATTTCCAGCG CGCCACAGTG 
ATCCGGCCAG AGAATCAGAG CAATCGCCAA CCAATGTTCT ACGTTCGCTT CATCGACTAT 
GGTAATTGCA CTTTGCTGCC CATGCAGCTA ATGCGTTTGA TGCCGAGAGA GCTAACTGAA 
CAGTACGGCG ACCTTCCGCC ACGTGTTTTT GAGTGCAGGC TGGCAATGGT TCAGCCCTCT 
TCAGTGGTCA GTGGAAACAA TAGGTGGTCC ACCGCGGCTA ATGATATGCT CAAGACAGTG 
GCCCAATGCG GTCTAATCGA TATTGAGGTG TATTCGCTTT TCAATAATGT AGCAGCTGTT 
CTCATTCATA TGAGAGATGG CATAATCAAC GATAAGCTAG TAGAGCTTAT GTTGTGCCGT 
CGATCCGACG AAGATTATAT GTCTAGAAAA GATCATGATT TCCGTTTGCG ACGTCAAGAA 
TCTGCCCGGA ACTTGAGCAC GGCGCAGCGT CAACAGATCA ATGAGGAATA TCTGCGATCC 
TGTCAGCTGC CGCAAGACCA CGATCTACCA CCGCCTCCGC TGGAAAAGTG CAAGACCGTT 
GTAATGTTGA AAGGACCCAA CAGTCCTTTG GAATGTACTA TGCGATCCAT TACTCGTGTG 
GGCTTAAGTA AACGGGTGAA TATAGACCAT TTGTCCGTTA ATGCACTTCT CCTGGATGCT 
GATCCTCAAG ATCACCACGA TCATTTGATA GTGGCCCACG AAATCGCGGA GAGTAGAAAC 
GGGCAGACAC TGACAGCCCG TGGAACCACC CTGATGCCCA ATGTGCAGGG ATTCGGTGCT 
CTAATGGTTA TGCTATTTAG TCCCACTATG CAACTTAAAT GCAATAAAGA AGGCACATCA 
TATGTCTCTG TTCTGGGAGG ATTGGGTTGT GACCCGGACA CAAACGAGCC CTACTTCGCA 
GAGCATGACG TGTTGATTAA TCTGGATGTC AACATTCTCG AAGATGATGT GATTTTGATT 
AATCAAATTC GCTACTATAT AGATAGTGTC TTCTTCAACT TTAAGGAAGA AAACAATCCA 
GCTGTTAGCG TAAATGAACG TGTTTCTATA TACACTCAGC TGCGTAGCCT TATAAATAGG 
CTCCTTTGCA AGGATCGTAG ATATATCGAA AGAAATATGA GCAATGCGGA TTTTGAATGG 
GAAACCAATC CCGAACTACC ACTGCCGAAT GAACCTTTTG GCAAGAGGGC CATATTTCCG 
ATGCACTCAC TTACTGAGCT CCAAGAGGAA GACACGGGTC GCTTGGTGCA ACTGAGAGAG 
AACTGCAGCA TGTTGCACAA ATGGCGCAAC TTCGAAGGCA CTTTACCCCA TATGACTTGC 
AAACTGTGCA ATCAGTTGTT GGAATCGGTT CCCCAGCTCC GTTTGCATCT CCTGACCATA 
TTGCATCGTG ATCGTGAAAA GCAAATCGAC TATTGCAACC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spn-E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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