toe cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	toe
Entrez Gene ID	39418
Full Name	twin of eyg
Synonyms	CG10704,Dmel\CG10704,Toe,anon-WO0172774.89
General protein information	Preferred Names twin of eyg Names CG10704 gene product from transcript CG10704-RB CG10704-PA CG10704-PB toe-PA toe-PB twin of eyegone
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 69C1-69C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274833.1	NP_001261762.1	twin of eyg, isoform B
NM_079317.3	NP_524041.2	twin of eyg, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Eyg makes differential use of several domains when compared to Toe, and the number of repressor domains also differs between the two Pax6(5a) homologs.
Title: Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila.

Data show that eyegone and its related gene, twin of eyegone (toe), are expressed in part of the anterior compartment of the adult mesothorax (notum).
Title: The Pax-homeobox gene eyegone is involved in the subdivision of the thorax of Drosophila.

The following toe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the toe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18354	NM_079317.3 Latest version!	Drosophila melanogaster twin of eyg (toe), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa20838	NM_001274833.1 Latest version!	Drosophila melanogaster twin of eyg (toe), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

toe ( NM_079317.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524041.2 Drosophila melanogaster twin of eyg (toe), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18354
Clone ID Related Accession (Same CDS sequence)	NM_079317.3
Accession Version	NM_079317.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twin of eyg, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079317.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAACCA GCACCTCACC CGGACCCACA AGGACAACTG CAGCCATTAG AGTCTCCCTG 
CCAGGAGCAG CACCCGCAAT TCCCGCTACC GCCACCGCCG GCTCTCAATC CCCCGGCGCC 
ATCATGCCCA TGGCCGCCGT GCCGTTGCCG TCGCATCTCC AACTGCTGGG CAGTTCCGCG 
GGAGGCGTCG GTCAGCCGGC GGCAATCACG CCCGTTTCGC CCACGGCTGC TACCACAGTT 
CTCGCCCAAC CGCACAGTTT GAGCCCCTCC ACGCCCATCC TCACCCAAGG AGCACCCCCT 
GCTGCGGCGT TGGGCGGCAT CTTCTGCGGA GGCAGTGCAG CAGCGGGACC AGGAGCAGCA 
GCGGCGGCGG CCACGAATCT CAACGCCCTG GCCTCGCAGC ATCGACTACT GGAGTTGTCC 
CGCTTCGGAC TGCGTGGATA TGATCTGGCG CAGCATATGC TATCCCAACA AGGAGCCGTA 
TCCAAGTTGC TGGGCACCCT GCGACCACCT GGCCTAATTG GCGGCTCCAA GCCGAAGGTG 
GCCACCCCGA CTGTCGTCTC CAAGATCGAG CAGTACAAGC GCGAGAATCC GACGATCTTT 
GCATGGGAAA TCCGCGAGCG ACTGATCACC GAAGGTGTCT GCACCAATGC CACTGCTCCT 
TCGGTGAGCA GCATCAATCG CATATTGCGT AATCGAGCGG CGGAAAGGGC AGCTGCGGAA 
TTTGCTCGGG CGGCGAGTTA CGGCTATGCC ATCCATCCCA CACATCCGCA TCCGTACACC 
AGTTTCCCCA CTTGGCCGGC GCATCATCCG CTGTGGGGAG CCGTGCCCCT GGCCACGCCA 
CCTGGTGGCG GCCCTGCTGG AGCCGGTGGT GCACTGCAGC CGGGCGGCAG TGGCAGCAGC 
TATGGCAGTG ATGGCAACAT GAGCTCAAAT CCCAATAGCA GCAACAGCAA CACCACCCAC 
AGCAATGGCC ACAATACCAA CAGCGGCAGT GGATGCGGGG ATAGTAGTGC CGGAAGTGGA 
CGCCTCTCCC TGCCGGCACT TTCGCCGGAT TCCGGAAGTA GGGACAGCCG CTCCCCAGAC 
GCAGATGCCA ATCGGATGAT AGATATCGAA GGCGAGGACA GCGAGTCGCA GGACAGTGAC 
CAGCCGAAGT TCCGGCGCAA TCGCACCACC TTCAGTCCGG AGCAGCTGGA TGAGCTGGAG 
AAGGAGTTCG ACAAGTCGCA CTATCCCTGC GTGAATACCC GCGAGAAACT GGCCGCCCGG 
ACGGCACTGA GCGAGGCCAG GGTGCAGGTT TGGTTTTCCA ACAGACGAGC GAAATGGCGG 
CGCCACCAGC GGGTCAACTT GATCAAGCAG CGCGACTCGC CCTCGACATC GAGCTCACCC 
ACGCCGTTGG TCAATCCGGT GGTCAGTCCG GTCAGTCCAA TCCCAGTTCC AGTTCCAGTT 
GCAGTTCCAG AATCTGGCCA ACAGAAGCAG CCATATCCGT ACAGCACCAG CAACATGTGC 
AACACCAGCA GCAGCAGCAG CAACAGTCAA CCGTGCAACA CCATCAATCC CGGCAGCAAA 
ATGAGCAGCA AAACCAGCAG CGTCAGCAGC AACCAGCACA TGGAAGAGCC AGCAGCGGCG 
GTGGCCACTG CCTCACCCAC AGCATCAGCT CCATTATCAA TGGGCGGTGA GAACAGTGCA 
TTTCGCGCTC TGCCCATGAC CTTGCCGATG CCCATGACCT TGCCCACGGC ATCGGCGGCG 
GCCTTCGCGC TCAGCTTCGC CCGCCAGTAC ATAGCCAAGT ACATGGGCAC GCCCCTCAAT 
CTGGGACATG GTTCGTCACC AATCCAGGCA CAGTCTGGAC GGGATCATCA GTCGGAGGAG 
CGAGAGTATG AGGATGAGCA GGAGGAGGAG GAGGTTATCA ATGTGGAGCA CGACGCCGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524041.2
CDS	356..2278
Translation	MATSTSPGPTRTTAAIRVSLPGAAPAIPATATAGSQSPGAIMPMAAVPLPSHLQLLGSSAGGVGQPAAITPVSPTAATTVLAQPHSLSPSTPILTQGAPPAAALGGIFCGGSAAAGPGAAAAAATNLNALASQHRLLELSRFGLRGYDLAQHMLSQQGAVSKLLGTLRPPGLIGGSKPKVATPTVVSKIEQYKRENPTIFAWEIRERLITEGVCTNATAPSVSSINRILRNRAAERAAAEFARAASYGYAIHPTHPHPYTSFPTWPAHHPLWGAVPLATPPGGGPAGAGGALQPGGSGSSYGSDGNMSSNPNSSNSNTTHSNGHNTNSGSGCGDSSAGSGRLSLPALSPDSGSRDSRSPDADANRMIDIEGEDSESQDSDQPKFRRNRTTFSPEQLDELEKEFDKSHYPCVNTREKLAARTALSEARVQVWFSNRRAKWRRHQRVNLIKQRDSPSTSSSPTPLVNPVVSPVSPIPVPVPVAVPESGQQKQPYPYSTSNMCNTSSSSSNSQPCNTINPGSKMSSKTSSVSSNQHMEEPAAAVATASPTASAPLSMGGENSAFRALPMTLPMPMTLPTASAAAFALSFARQYIAKYMGTPLNLGHGSSPIQAQSGRDHQSEEREYEDEQEEEEVINVEHDAE

Target ORF information:

RefSeq Version	NM_079317.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twin of eyg (toe), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079317.3

ORF Insert Sequence:

ATGGCAACCA GCACCTCACC CGGACCCACA AGGACAACTG CAGCCATTAG AGTCTCCCTG 
CCAGGAGCAG CACCCGCAAT TCCCGCTACC GCCACCGCCG GCTCTCAATC CCCCGGCGCC 
ATCATGCCCA TGGCCGCCGT GCCGTTGCCG TCGCATCTCC AACTGCTGGG CAGTTCCGCG 
GGAGGCGTCG GTCAGCCGGC GGCAATCACG CCCGTTTCGC CCACGGCTGC TACCACAGTT 
CTCGCCCAAC CGCACAGTTT GAGCCCCTCC ACGCCCATCC TCACCCAAGG AGCACCCCCT 
GCTGCGGCGT TGGGCGGCAT CTTCTGCGGA GGCAGTGCAG CAGCGGGACC AGGAGCAGCA 
GCGGCGGCGG CCACGAATCT CAACGCCCTG GCCTCGCAGC ATCGACTACT GGAGTTGTCC 
CGCTTCGGAC TGCGTGGATA TGATCTGGCG CAGCATATGC TATCCCAACA AGGAGCCGTA 
TCCAAGTTGC TGGGCACCCT GCGACCACCT GGCCTAATTG GCGGCTCCAA GCCGAAGGTG 
GCCACCCCGA CTGTCGTCTC CAAGATCGAG CAGTACAAGC GCGAGAATCC GACGATCTTT 
GCATGGGAAA TCCGCGAGCG ACTGATCACC GAAGGTGTCT GCACCAATGC CACTGCTCCT 
TCGGTGAGCA GCATCAATCG CATATTGCGT AATCGAGCGG CGGAAAGGGC AGCTGCGGAA 
TTTGCTCGGG CGGCGAGTTA CGGCTATGCC ATCCATCCCA CACATCCGCA TCCGTACACC 
AGTTTCCCCA CTTGGCCGGC GCATCATCCG CTGTGGGGAG CCGTGCCCCT GGCCACGCCA 
CCTGGTGGCG GCCCTGCTGG AGCCGGTGGT GCACTGCAGC CGGGCGGCAG TGGCAGCAGC 
TATGGCAGTG ATGGCAACAT GAGCTCAAAT CCCAATAGCA GCAACAGCAA CACCACCCAC 
AGCAATGGCC ACAATACCAA CAGCGGCAGT GGATGCGGGG ATAGTAGTGC CGGAAGTGGA 
CGCCTCTCCC TGCCGGCACT TTCGCCGGAT TCCGGAAGTA GGGACAGCCG CTCCCCAGAC 
GCAGATGCCA ATCGGATGAT AGATATCGAA GGCGAGGACA GCGAGTCGCA GGACAGTGAC 
CAGCCGAAGT TCCGGCGCAA TCGCACCACC TTCAGTCCGG AGCAGCTGGA TGAGCTGGAG 
AAGGAGTTCG ACAAGTCGCA CTATCCCTGC GTGAATACCC GCGAGAAACT GGCCGCCCGG 
ACGGCACTGA GCGAGGCCAG GGTGCAGGTT TGGTTTTCCA ACAGACGAGC GAAATGGCGG 
CGCCACCAGC GGGTCAACTT GATCAAGCAG CGCGACTCGC CCTCGACATC GAGCTCACCC 
ACGCCGTTGG TCAATCCGGT GGTCAGTCCG GTCAGTCCAA TCCCAGTTCC AGTTCCAGTT 
GCAGTTCCAG AATCTGGCCA ACAGAAGCAG CCATATCCGT ACAGCACCAG CAACATGTGC 
AACACCAGCA GCAGCAGCAG CAACAGTCAA CCGTGCAACA CCATCAATCC CGGCAGCAAA 
ATGAGCAGCA AAACCAGCAG CGTCAGCAGC AACCAGCACA TGGAAGAGCC AGCAGCGGCG 
GTGGCCACTG CCTCACCCAC AGCATCAGCT CCATTATCAA TGGGCGGTGA GAACAGTGCA 
TTTCGCGCTC TGCCCATGAC CTTGCCGATG CCCATGACCT TGCCCACGGC ATCGGCGGCG 
GCCTTCGCGC TCAGCTTCGC CCGCCAGTAC ATAGCCAAGT ACATGGGCAC GCCCCTCAAT 
CTGGGACATG GTTCGTCACC AATCCAGGCA CAGTCTGGAC GGGATCATCA GTCGGAGGAG 
CGAGAGTATG AGGATGAGCA GGAGGAGGAG GAGGTTATCA ATGTGGAGCA CGACGCCGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

toe ( NM_001274833.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261762.1 Drosophila melanogaster twin of eyg (toe), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa20838
Clone ID Related Accession (Same CDS sequence)	NM_001274833.1
Accession Version	NM_001274833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twin of eyg, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAACCA GCACCTCACC CGGACCCACA AGGACAACTG CAGCCATTAG AGTCTCCCTG 
CCAGGAGCAG CACCCGCAAT TCCCGCTACC GCCACCGCCG GCTCTCAATC CCCCGGCGCC 
ATCATGCCCA TGGCCGCCGT GCCGTTGCCG TCGCATCTCC AACTGCTGGG CAGTTCCGCG 
GGAGGCGTCG GTCAGCCGGC GGCAATCACG CCCGTTTCGC CCACGGCTGC TACCACAGTT 
CTCGCCCAAC CGCACAGTTT GAGCCCCTCC ACGCCCATCC TCACCCAAGG AGCACCCCCT 
GCTGCGGCGT TGGGCGGCAT CTTCTGCGGA GGCAGTGCAG CAGCGGGACC AGGAGCAGCA 
GCGGCGGCGG CCACGAATCT CAACGCCCTG GCCTCGCAGC ATCGACTACT GGAGTTGTCC 
CGCTTCGGAC TGCGTGGATA TGATCTGGCG CAGCATATGC TATCCCAACA AGGAGCCGTA 
TCCAAGTTGC TGGGCACCCT GCGACCACCT GGCCTAATTG GCGGCTCCAA GCCGAAGGTG 
GCCACCCCGA CTGTCGTCTC CAAGATCGAG CAGTACAAGC GCGAGAATCC GACGATCTTT 
GCATGGGAAA TCCGCGAGCG ACTGATCACC GAAGGTGTCT GCACCAATGC CACTGCTCCT 
TCGGTGAGCA GCATCAATCG CATATTGCGT AATCGAGCGG CGGAAAGGGC AGCTGCGGAA 
TTTGCTCGGG CGGCGAGTTA CGGCTATGCC ATCCATCCCA CACATCCGCA TCCGTACACC 
AGTTTCCCCA CTTGGCCGGC GCATCATCCG CTGTGGGGAG CCGTGCCCCT GGCCACGCCA 
CCTGGTGGCG GCCCTGCTGG AGCCGGTGGT GCACTGCAGC CGGGCGGCAG TGGCAGCAGC 
TATGGCAGTG ATGGCAACAT GAGCTCAAAT CCCAATAGCA GCAACAGCAA CACCACCCAC 
AGCAATGGCC ACAATACCAA CAGCGGCAGT GGATGCGGGG ATAGTAGTGC CGGAAGTGGA 
CGCCTCTCCC TGCCGGCACT TTCGCCGGAT TCCGGAAGTA GGGACAGCCG CTCCCCAGAC 
GCAGATGCCA ATCGGATGAT AGATATCGAA GGCGAGGACA GCGAGTCGCA GGACAGTGAC 
CAGCCGAAGT TCCGGCGCAA TCGCACCACC TTCAGTCCGG AGCAGCTGGA TGAGCTGGAG 
AAGGAGTTCG ACAAGTCGCA CTATCCCTGC GTGAATACCC GCGAGAAACT GGCCGCCCGG 
ACGGCACTGA GCGAGGCCAG GGTGCAGACG AGCGAAATGG CGGCGCCACC AGCGGGTCAA 
CTTGATCAAG CAGCGCGACT CGCCCTCGAC ATCGAGCTCA CCCACGCCGT TGGTCAATCC 
GGTGGTCAGT CCGGTCAGTC CAATCCCAGT TCCAGTTCCA GTTGCAGTTC CAGAATCTGG 
CCAACAGAAG CAGCCATATC CGTACAGCAC CAGCAACATG TGCAACACCA GCAGCAGCAG 
CAGCAACAGT CAACCGTGCA ACACCATCAA TCCCGGCAGC AAAATGAGCA GCAAAACCAG 
CAGCGTCAGC AGCAACCAGC ACATGGAAGA GCCAGCAGCG GCGGTGGCCA CTGCCTCACC 
CACAGCATCA GCTCCATTAT CAATGGGCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261762.1
CDS	356..2008
Translation	MATSTSPGPTRTTAAIRVSLPGAAPAIPATATAGSQSPGAIMPMAAVPLPSHLQLLGSSAGGVGQPAAITPVSPTAATTVLAQPHSLSPSTPILTQGAPPAAALGGIFCGGSAAAGPGAAAAAATNLNALASQHRLLELSRFGLRGYDLAQHMLSQQGAVSKLLGTLRPPGLIGGSKPKVATPTVVSKIEQYKRENPTIFAWEIRERLITEGVCTNATAPSVSSINRILRNRAAERAAAEFARAASYGYAIHPTHPHPYTSFPTWPAHHPLWGAVPLATPPGGGPAGAGGALQPGGSGSSYGSDGNMSSNPNSSNSNTTHSNGHNTNSGSGCGDSSAGSGRLSLPALSPDSGSRDSRSPDADANRMIDIEGEDSESQDSDQPKFRRNRTTFSPEQLDELEKEFDKSHYPCVNTREKLAARTALSEARVQTSEMAAPPAGQLDQAARLALDIELTHAVGQSGGQSGQSNPSSSSSCSSRIWPTEAAISVQHQQHVQHQQQQQQQSTVQHHQSRQQNEQQNQQRQQQPAHGRASSGGGHCLTHSISSIINGR

Target ORF information:

RefSeq Version	NM_001274833.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twin of eyg (toe), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274833.1

ORF Insert Sequence:

ATGGCAACCA GCACCTCACC CGGACCCACA AGGACAACTG CAGCCATTAG AGTCTCCCTG 
CCAGGAGCAG CACCCGCAAT TCCCGCTACC GCCACCGCCG GCTCTCAATC CCCCGGCGCC 
ATCATGCCCA TGGCCGCCGT GCCGTTGCCG TCGCATCTCC AACTGCTGGG CAGTTCCGCG 
GGAGGCGTCG GTCAGCCGGC GGCAATCACG CCCGTTTCGC CCACGGCTGC TACCACAGTT 
CTCGCCCAAC CGCACAGTTT GAGCCCCTCC ACGCCCATCC TCACCCAAGG AGCACCCCCT 
GCTGCGGCGT TGGGCGGCAT CTTCTGCGGA GGCAGTGCAG CAGCGGGACC AGGAGCAGCA 
GCGGCGGCGG CCACGAATCT CAACGCCCTG GCCTCGCAGC ATCGACTACT GGAGTTGTCC 
CGCTTCGGAC TGCGTGGATA TGATCTGGCG CAGCATATGC TATCCCAACA AGGAGCCGTA 
TCCAAGTTGC TGGGCACCCT GCGACCACCT GGCCTAATTG GCGGCTCCAA GCCGAAGGTG 
GCCACCCCGA CTGTCGTCTC CAAGATCGAG CAGTACAAGC GCGAGAATCC GACGATCTTT 
GCATGGGAAA TCCGCGAGCG ACTGATCACC GAAGGTGTCT GCACCAATGC CACTGCTCCT 
TCGGTGAGCA GCATCAATCG CATATTGCGT AATCGAGCGG CGGAAAGGGC AGCTGCGGAA 
TTTGCTCGGG CGGCGAGTTA CGGCTATGCC ATCCATCCCA CACATCCGCA TCCGTACACC 
AGTTTCCCCA CTTGGCCGGC GCATCATCCG CTGTGGGGAG CCGTGCCCCT GGCCACGCCA 
CCTGGTGGCG GCCCTGCTGG AGCCGGTGGT GCACTGCAGC CGGGCGGCAG TGGCAGCAGC 
TATGGCAGTG ATGGCAACAT GAGCTCAAAT CCCAATAGCA GCAACAGCAA CACCACCCAC 
AGCAATGGCC ACAATACCAA CAGCGGCAGT GGATGCGGGG ATAGTAGTGC CGGAAGTGGA 
CGCCTCTCCC TGCCGGCACT TTCGCCGGAT TCCGGAAGTA GGGACAGCCG CTCCCCAGAC 
GCAGATGCCA ATCGGATGAT AGATATCGAA GGCGAGGACA GCGAGTCGCA GGACAGTGAC 
CAGCCGAAGT TCCGGCGCAA TCGCACCACC TTCAGTCCGG AGCAGCTGGA TGAGCTGGAG 
AAGGAGTTCG ACAAGTCGCA CTATCCCTGC GTGAATACCC GCGAGAAACT GGCCGCCCGG 
ACGGCACTGA GCGAGGCCAG GGTGCAGACG AGCGAAATGG CGGCGCCACC AGCGGGTCAA 
CTTGATCAAG CAGCGCGACT CGCCCTCGAC ATCGAGCTCA CCCACGCCGT TGGTCAATCC 
GGTGGTCAGT CCGGTCAGTC CAATCCCAGT TCCAGTTCCA GTTGCAGTTC CAGAATCTGG 
CCAACAGAAG CAGCCATATC CGTACAGCAC CAGCAACATG TGCAACACCA GCAGCAGCAG 
CAGCAACAGT CAACCGTGCA ACACCATCAA TCCCGGCAGC AAAATGAGCA GCAAAACCAG 
CAGCGTCAGC AGCAACCAGC ACATGGAAGA GCCAGCAGCG GCGGTGGCCA CTGCCTCACC 
CACAGCATCA GCTCCATTAT CAATGGGCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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toe cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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