Dronc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dronc
Entrez Gene ID	39173
Full Name	Death regulator Nedd2-like caspase
Synonyms	CG8090,CG8091,DRONC,Dmel\CG8091,Dronc/Casp9,Nc,Nc\Dronc,anon-EST:GreesD6,anon-EST:GressD6,anon-WO0118547.313,caspase-9,dronc
General protein information	Preferred Names Death regulator Nedd2-like caspase Names CG8091 gene product from transcript CG8091-RA CG8091-PA Caspase-9-like Dronc-PA Nedd2-like caspase caspase-9 death regulator Nedd2-like caspase dronc drosophila Caspase-9 homologue drosophila Nedd2-like caspase nedd 2-like caspase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67D2-67D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079293.4	NP_524017.1	death regulator Nedd2-like caspase

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Nc	NP_524017.1
Anopheles gambiae (African malaria mosquito)	CASPL2	XP_318061.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

e provide evidence that not all of the non-apoptotic functions of Dronc require its catalytic activity. In conclusion, we demonstrate a mechanism whereby Dronc's apoptotic and non-apoptotic activities can be kept silenced in a non-degradative manner through a single ubiquitylation event in living cells.
Title: An inhibitory mono-ubiquitylation of the Drosophila initiator caspase Dronc functions in both apoptotic and non-apoptotic pathways.

Dronc protein fails to accumulate in epithelial cells with impaired proteasome function suggesting that it is not degraded by the proteasome.
Title: The initiator caspase Dronc is subject of enhanced autophagy upon proteasome impairment in Drosophila.

the principal caspase required for the cleavage of Inhibitor of Apoptosis 2 Protein
Title: Independent Proteolytic Activities Control the Stability and Size of Drosophila Inhibitor of Apoptosis 2 Protein.

Findings suggest that the intrinsic properties of initiator caspase Dronc proteinsare responsible, at least in part, for the differing sensitivity of Ld652Y and BM-N to apoptosis induction upon nucleopolyhedroviruses (NPVs) infection.
Title: Cloning and functional characterization of the Lymantria dispar initiator caspase dronc.

that DRONC protein accumulates in "undead" cells due to increased transcription of dronc in these cells
Title: Drosophila IAP1-mediated ubiquitylation controls activation of the initiator caspase DRONC independent of protein degradation.

The following Dronc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dronc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18136	NM_079293.4 Latest version!	Drosophila melanogaster death regulator Nedd2-like caspase (Dronc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dronc ( NM_079293.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524017.1 Drosophila melanogaster death regulator Nedd2-like caspase (Dronc), mRNA.

Price & Availability↑

CloneID	OFa18136
Clone ID Related Accession (Same CDS sequence)	NM_079293.4
Accession Version	NM_079293.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	death regulator Nedd2-like caspase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079293.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCCGC CGGAGCTCGA GATTGGAATG CCGAAGAGGC ATCGCGAGCA TATACGCAAG 
AATCTGAATA TACTGGTTGA ATGGACGAAC TACGAGCGTC TGGCCATGGA GTGCGTGCAA 
CAGGGCATCC TAACCGTTCA GATGCTAAGA AATACGCAGG ATCTCAATGG CAAGCCATTC 
AACATGGACG AGAAGGATGT GCGTGTGGAG CAGCATCGTA GGCTCCTATT GAAGATCACC 
CAGCGTGGTC CCACCGCCTA TAACCTGCTG ATCAATGCAC TGCGCAATAT CAATTGTCTG 
GATGCGGCCG TTCTATTGGA ATCCGTCGAT GAGTCCGATT CAAGGCCACC CTTTATCTCG 
CTAAACGAAC GGAGAACCAG CCGGAAGTCG GCCGATATTG TGGACACACC CTCACCCGAA 
GCCTCCGAAG GACCCTGCGT TAGCAAGCTC CGGAATGAGC CGCTGGGAGC ACTCACCCCT 
TATGTGGGTG TCGTTGACGG TCCCGAGGTA AAAAAATCGA AAAAGATACA TGGTGGGGAT 
AGTGCCATAT TGGGCACATA TAAGATGCAA TCACGTTTCA ACCGAGGCGT TTTGCTAATG 
GTTAACATAA TGGACTATCC GGATCAAAAC CGTCGACGGA TCGGAGCCGA AAAGGACAGC 
AAGTCGTTGA TACACTTGTT TCAAGAACTG AATTTTACGA TTTTCCCCTA TGGGAACGTG 
AATCAGGATC AGTTCTTTAA ACTTCTGACA ATGGTGACCT CCTCGTCGTA TGTGCAGAAT 
ACCGAGTGTT TCGTAATGGT ACTGATGACA CACGGCAACA GTGTGGAGGG AAAAGAGAAG 
GTGGAGTTTT GCGATGGATC TGTGGTCGAT ATGCAGAAGA TCAAGGACCA TTTCCAGACG 
GCCAAATGTC CTTATTTGGT GAACAAGCCG AAGGTGCTTA TGTTTCCCTT TTGCCGCGGC 
GATGAATATG ATTTGGGCCA TCCAAAGAAT CAAGGCAATC TCATGGAGCC AGTGTATACG 
GCGCAAGAGG AGAAGTGGCC TGACACCCAG ACGGAGGGCA TACCCAGCCC GAGCACCAAT 
GTGCCAAGTC TTGCCGACAC TCTGGTCTGC TATGCTAATA CGCCGGGCTA TGTTACCCAC 
CGCGATCTCG ACACGGGCAG CTGGTACATC CAGAAGTTTT GCCAAGTGAT GGCCGATCAT 
GCCCACGACA CAGACCTTGA GGATATCCTA AAGAAGACGA GCGAAGCCGT GGGTAATAAG 
CGCACCAAGA AGGGTTCCAT GCAGACAGGT GCCTATGATA ATCTTGGCTT TAATAAGAAA 
CTCTACTTCA ATCCCGGGTT TTTCAACGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524017.1
CDS	410..1762
Translation	MQPPELEIGMPKRHREHIRKNLNILVEWTNYERLAMECVQQGILTVQMLRNTQDLNGKPFNMDEKDVRVEQHRRLLLKITQRGPTAYNLLINALRNINCLDAAVLLESVDESDSRPPFISLNERRTSRKSADIVDTPSPEASEGPCVSKLRNEPLGALTPYVGVVDGPEVKKSKKIHGGDSAILGTYKMQSRFNRGVLLMVNIMDYPDQNRRRIGAEKDSKSLIHLFQELNFTIFPYGNVNQDQFFKLLTMVTSSSYVQNTECFVMVLMTHGNSVEGKEKVEFCDGSVVDMQKIKDHFQTAKCPYLVNKPKVLMFPFCRGDEYDLGHPKNQGNLMEPVYTAQEEKWPDTQTEGIPSPSTNVPSLADTLVCYANTPGYVTHRDLDTGSWYIQKFCQVMADHAHDTDLEDILKKTSEAVGNKRTKKGSMQTGAYDNLGFNKKLYFNPGFFNE

Target ORF information:

RefSeq Version	NM_079293.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster death regulator Nedd2-like caspase (Dronc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079293.4

ORF Insert Sequence:

ATGCAGCCGC CGGAGCTCGA GATTGGAATG CCGAAGAGGC ATCGCGAGCA TATACGCAAG 
AATCTGAATA TACTGGTTGA ATGGACGAAC TACGAGCGTC TGGCCATGGA GTGCGTGCAA 
CAGGGCATCC TAACCGTTCA GATGCTAAGA AATACGCAGG ATCTCAATGG CAAGCCATTC 
AACATGGACG AGAAGGATGT GCGTGTGGAG CAGCATCGTA GGCTCCTATT GAAGATCACC 
CAGCGTGGTC CCACCGCCTA TAACCTGCTG ATCAATGCAC TGCGCAATAT CAATTGTCTG 
GATGCGGCCG TTCTATTGGA ATCCGTCGAT GAGTCCGATT CAAGGCCACC CTTTATCTCG 
CTAAACGAAC GGAGAACCAG CCGGAAGTCG GCCGATATTG TGGACACACC CTCACCCGAA 
GCCTCCGAAG GACCCTGCGT TAGCAAGCTC CGGAATGAGC CGCTGGGAGC ACTCACCCCT 
TATGTGGGTG TCGTTGACGG TCCCGAGGTA AAAAAATCGA AAAAGATACA TGGTGGGGAT 
AGTGCCATAT TGGGCACATA TAAGATGCAA TCACGTTTCA ACCGAGGCGT TTTGCTAATG 
GTTAACATAA TGGACTATCC GGATCAAAAC CGTCGACGGA TCGGAGCCGA AAAGGACAGC 
AAGTCGTTGA TACACTTGTT TCAAGAACTG AATTTTACGA TTTTCCCCTA TGGGAACGTG 
AATCAGGATC AGTTCTTTAA ACTTCTGACA ATGGTGACCT CCTCGTCGTA TGTGCAGAAT 
ACCGAGTGTT TCGTAATGGT ACTGATGACA CACGGCAACA GTGTGGAGGG AAAAGAGAAG 
GTGGAGTTTT GCGATGGATC TGTGGTCGAT ATGCAGAAGA TCAAGGACCA TTTCCAGACG 
GCCAAATGTC CTTATTTGGT GAACAAGCCG AAGGTGCTTA TGTTTCCCTT TTGCCGCGGC 
GATGAATATG ATTTGGGCCA TCCAAAGAAT CAAGGCAATC TCATGGAGCC AGTGTATACG 
GCGCAAGAGG AGAAGTGGCC TGACACCCAG ACGGAGGGCA TACCCAGCCC GAGCACCAAT 
GTGCCAAGTC TTGCCGACAC TCTGGTCTGC TATGCTAATA CGCCGGGCTA TGTTACCCAC 
CGCGATCTCG ACACGGGCAG CTGGTACATC CAGAAGTTTT GCCAAGTGAT GGCCGATCAT 
GCCCACGACA CAGACCTTGA GGATATCCTA AAGAAGACGA GCGAAGCCGT GGGTAATAAG 
CGCACCAAGA AGGGTTCCAT GCAGACAGGT GCCTATGATA ATCTTGGCTT TAATAAGAAA 
CTCTACTTCA ATCCCGGGTT TTTCAACGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dronc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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