vsg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	vsg
Entrez Gene ID	39137
Full Name	visgun
Synonyms	BEST:GH15083,CG16707,Dmel\CG16707,FBgn0045823,GH15083,Vsg,anon-WO0118547.164
General protein information	Preferred Names visgun Names CG16707 gene product from transcript CG16707-RD CG16707-PA CG16707-PB CG16707-PC CG16707-PD CG16707-PE endolyn vsg-PA vsg-PB vsg-PC vsg-PD vsg-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67C5-67C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274717.1	NP_001261646.1	visgun, isoform E
NM_168363.2	NP_729535.1	visgun, isoform A
NM_168362.2	NP_729534.1	visgun, isoform D
NM_140092.2	NP_648349.1	visgun, isoform C
NM_168364.2	NP_729536.1	visgun, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate that endolyn is an evolutionarily conserved sialomucin fundamentally involved in cell proliferation in both the human and Drosophila melanogaster.
Title: The Drosophila ortholog of the endolysosomal membrane protein, endolyn, regulates cell proliferation.

The following vsg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vsg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18086	NM_140092.2 Latest version!	Drosophila melanogaster visgun (vsg), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18086	NM_168362.2 Latest version!	Drosophila melanogaster visgun (vsg), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18087	NM_168364.2 Latest version!	Drosophila melanogaster visgun (vsg), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18087	NM_168363.2 Latest version!	Drosophila melanogaster visgun (vsg), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa20805	NM_001274717.1 Latest version!	Drosophila melanogaster visgun (vsg), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vsg ( NM_168362.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729534.1 Drosophila melanogaster visgun (vsg), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa18086
Clone ID Related Accession (Same CDS sequence)	NM_168362.2 , NM_140092.2
Accession Version	NM_168362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	visgun, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168362.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCGGC AGGCGAAATT CCTAATCTTG TGCCTCTTTG TGGGCCTCTT CTCCGCGAAT 
TTGTGCGAAG AAGCAGTGAC CACACCGGCT CCAGCGGACA CCACCACCCA GGAATCCAAG 
AACACCACCA CTCCGCCGGA CACCACCACT ACTGTGACGC CACCGTCGAC CTCAACCACG 
AGCACCACAA CTGAAAAGAC CACGACGACG CCACCAATTA CCACATCGAC TGAGAAGACC 
ACAACTTCCA CTACTCCAGC CAGCACCACC AGCAGCACCA CTCCGGCCAG CACCACCAGC 
AGCACCACTC CGGCAACGAC CACCACCACG CCTGGAACTA CATCTACGAC CACTCCGTCT 
CCGAACTCGA CCACAACTAC GCCGCCGCCA CACACATCAA CCACTCCGGC GCCGAAGCCC 
GTGCCGTGCG GTCATTTCGA TGGATCCTCG TTCATTGGCG GCATTGTGCT GACTCTGGGC 
CTGCTCGCTA TCGGCTTAGT GGCCTACAAG TTCTACAAGG CCCGCAACGA GCGCAACTAC 
CACACCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729534.1
CDS	228..779
Translation	MNRQAKFLILCLFVGLFSANLCEEAVTTPAPADTTTQESKNTTTPPDTTTTVTPPSTSTTSTTTEKTTTTPPITTSTEKTTTSTTPASTTSSTTPASTTSSTTPATTTTTPGTTSTTTPSPNSTTTTPPPHTSTTPAPKPVPCGHFDGSSFIGGIVLTLGLLAIGLVAYKFYKARNERNYHTL

Target ORF information:

RefSeq Version	NM_168362.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster visgun (vsg), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168362.2

ORF Insert Sequence:

ATGAATCGGC AGGCGAAATT CCTAATCTTG TGCCTCTTTG TGGGCCTCTT CTCCGCGAAT 
TTGTGCGAAG AAGCAGTGAC CACACCGGCT CCAGCGGACA CCACCACCCA GGAATCCAAG 
AACACCACCA CTCCGCCGGA CACCACCACT ACTGTGACGC CACCGTCGAC CTCAACCACG 
AGCACCACAA CTGAAAAGAC CACGACGACG CCACCAATTA CCACATCGAC TGAGAAGACC 
ACAACTTCCA CTACTCCAGC CAGCACCACC AGCAGCACCA CTCCGGCCAG CACCACCAGC 
AGCACCACTC CGGCAACGAC CACCACCACG CCTGGAACTA CATCTACGAC CACTCCGTCT 
CCGAACTCGA CCACAACTAC GCCGCCGCCA CACACATCAA CCACTCCGGC GCCGAAGCCC 
GTGCCGTGCG GTCATTTCGA TGGATCCTCG TTCATTGGCG GCATTGTGCT GACTCTGGGC 
CTGCTCGCTA TCGGCTTAGT GGCCTACAAG TTCTACAAGG CCCGCAACGA GCGCAACTAC 
CACACCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vsg ( NM_140092.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648349.1 Drosophila melanogaster visgun (vsg), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18086
Clone ID Related Accession (Same CDS sequence)	NM_168362.2 , NM_140092.2
Accession Version	NM_140092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	visgun, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140092.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCGGC AGGCGAAATT CCTAATCTTG TGCCTCTTTG TGGGCCTCTT CTCCGCGAAT 
TTGTGCGAAG AAGCAGTGAC CACACCGGCT CCAGCGGACA CCACCACCCA GGAATCCAAG 
AACACCACCA CTCCGCCGGA CACCACCACT ACTGTGACGC CACCGTCGAC CTCAACCACG 
AGCACCACAA CTGAAAAGAC CACGACGACG CCACCAATTA CCACATCGAC TGAGAAGACC 
ACAACTTCCA CTACTCCAGC CAGCACCACC AGCAGCACCA CTCCGGCCAG CACCACCAGC 
AGCACCACTC CGGCAACGAC CACCACCACG CCTGGAACTA CATCTACGAC CACTCCGTCT 
CCGAACTCGA CCACAACTAC GCCGCCGCCA CACACATCAA CCACTCCGGC GCCGAAGCCC 
GTGCCGTGCG GTCATTTCGA TGGATCCTCG TTCATTGGCG GCATTGTGCT GACTCTGGGC 
CTGCTCGCTA TCGGCTTAGT GGCCTACAAG TTCTACAAGG CCCGCAACGA GCGCAACTAC 
CACACCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648349.1
CDS	122..673
Translation	MNRQAKFLILCLFVGLFSANLCEEAVTTPAPADTTTQESKNTTTPPDTTTTVTPPSTSTTSTTTEKTTTTPPITTSTEKTTTSTTPASTTSSTTPASTTSSTTPATTTTTPGTTSTTTPSPNSTTTTPPPHTSTTPAPKPVPCGHFDGSSFIGGIVLTLGLLAIGLVAYKFYKARNERNYHTL

Target ORF information:

RefSeq Version	NM_140092.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster visgun (vsg), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140092.2

ORF Insert Sequence:

ATGAATCGGC AGGCGAAATT CCTAATCTTG TGCCTCTTTG TGGGCCTCTT CTCCGCGAAT 
TTGTGCGAAG AAGCAGTGAC CACACCGGCT CCAGCGGACA CCACCACCCA GGAATCCAAG 
AACACCACCA CTCCGCCGGA CACCACCACT ACTGTGACGC CACCGTCGAC CTCAACCACG 
AGCACCACAA CTGAAAAGAC CACGACGACG CCACCAATTA CCACATCGAC TGAGAAGACC 
ACAACTTCCA CTACTCCAGC CAGCACCACC AGCAGCACCA CTCCGGCCAG CACCACCAGC 
AGCACCACTC CGGCAACGAC CACCACCACG CCTGGAACTA CATCTACGAC CACTCCGTCT 
CCGAACTCGA CCACAACTAC GCCGCCGCCA CACACATCAA CCACTCCGGC GCCGAAGCCC 
GTGCCGTGCG GTCATTTCGA TGGATCCTCG TTCATTGGCG GCATTGTGCT GACTCTGGGC 
CTGCTCGCTA TCGGCTTAGT GGCCTACAAG TTCTACAAGG CCCGCAACGA GCGCAACTAC 
CACACCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vsg ( NM_168363.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729535.1 Drosophila melanogaster visgun (vsg), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18087
Clone ID Related Accession (Same CDS sequence)	NM_168363.2 , NM_168364.2
Accession Version	NM_168363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	visgun, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168363.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCGGC AGGCGAAATT CCTAATCTTG TGCCTCTTTG TGGGCCTCTT CTCCGCGAAT 
TTGTGCGAAG AAGTGACCAC ACCGGCTCCA GCGGACACCA CCACCCAGGA ATCCAAGAAC 
ACCACCACTC CGCCGGACAC CACCACTACT GTGACGCCAC CGTCGACCTC AACCACGAGC 
ACCACAACTG AAAAGACCAC GACGACGCCA CCAATTACCA CATCGACTGA GAAGACCACA 
ACTTCCACTA CTCCAGCCAG CACCACCAGC AGCACCACTC CGGCCAGCAC CACCAGCAGC 
ACCACTCCGG CAACGACCAC CACCACGCCT GGAACTACAT CTACGACCAC TCCGTCTCCG 
AACTCGACCA CAACTACGCC GCCGCCACAC ACATCAACCA CTCCGGCGCC GAAGCCCGTG 
CCGTGCGGTC ATTTCGATGG ATCCTCGTTC ATTGGCGGCA TTGTGCTGAC TCTGGGCCTG 
CTCGCTATCG GCTTAGTGGC CTACAAGTTC TACAAGGCCC GCAACGAGCG CAACTACCAC 
ACCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729535.1
CDS	228..776
Translation	MNRQAKFLILCLFVGLFSANLCEEVTTPAPADTTTQESKNTTTPPDTTTTVTPPSTSTTSTTTEKTTTTPPITTSTEKTTTSTTPASTTSSTTPASTTSSTTPATTTTTPGTTSTTTPSPNSTTTTPPPHTSTTPAPKPVPCGHFDGSSFIGGIVLTLGLLAIGLVAYKFYKARNERNYHTL

Target ORF information:

RefSeq Version	NM_168363.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster visgun (vsg), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168363.2

ORF Insert Sequence:

ATGAATCGGC AGGCGAAATT CCTAATCTTG TGCCTCTTTG TGGGCCTCTT CTCCGCGAAT 
TTGTGCGAAG AAGTGACCAC ACCGGCTCCA GCGGACACCA CCACCCAGGA ATCCAAGAAC 
ACCACCACTC CGCCGGACAC CACCACTACT GTGACGCCAC CGTCGACCTC AACCACGAGC 
ACCACAACTG AAAAGACCAC GACGACGCCA CCAATTACCA CATCGACTGA GAAGACCACA 
ACTTCCACTA CTCCAGCCAG CACCACCAGC AGCACCACTC CGGCCAGCAC CACCAGCAGC 
ACCACTCCGG CAACGACCAC CACCACGCCT GGAACTACAT CTACGACCAC TCCGTCTCCG 
AACTCGACCA CAACTACGCC GCCGCCACAC ACATCAACCA CTCCGGCGCC GAAGCCCGTG 
CCGTGCGGTC ATTTCGATGG ATCCTCGTTC ATTGGCGGCA TTGTGCTGAC TCTGGGCCTG 
CTCGCTATCG GCTTAGTGGC CTACAAGTTC TACAAGGCCC GCAACGAGCG CAACTACCAC 
ACCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vsg ( NM_168364.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729536.1 Drosophila melanogaster visgun (vsg), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18087
Clone ID Related Accession (Same CDS sequence)	NM_168363.2 , NM_168364.2
Accession Version	NM_168364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	visgun, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168364.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCGGC AGGCGAAATT CCTAATCTTG TGCCTCTTTG TGGGCCTCTT CTCCGCGAAT 
TTGTGCGAAG AAGTGACCAC ACCGGCTCCA GCGGACACCA CCACCCAGGA ATCCAAGAAC 
ACCACCACTC CGCCGGACAC CACCACTACT GTGACGCCAC CGTCGACCTC AACCACGAGC 
ACCACAACTG AAAAGACCAC GACGACGCCA CCAATTACCA CATCGACTGA GAAGACCACA 
ACTTCCACTA CTCCAGCCAG CACCACCAGC AGCACCACTC CGGCCAGCAC CACCAGCAGC 
ACCACTCCGG CAACGACCAC CACCACGCCT GGAACTACAT CTACGACCAC TCCGTCTCCG 
AACTCGACCA CAACTACGCC GCCGCCACAC ACATCAACCA CTCCGGCGCC GAAGCCCGTG 
CCGTGCGGTC ATTTCGATGG ATCCTCGTTC ATTGGCGGCA TTGTGCTGAC TCTGGGCCTG 
CTCGCTATCG GCTTAGTGGC CTACAAGTTC TACAAGGCCC GCAACGAGCG CAACTACCAC 
ACCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729536.1
CDS	122..670
Translation	MNRQAKFLILCLFVGLFSANLCEEVTTPAPADTTTQESKNTTTPPDTTTTVTPPSTSTTSTTTEKTTTTPPITTSTEKTTTSTTPASTTSSTTPASTTSSTTPATTTTTPGTTSTTTPSPNSTTTTPPPHTSTTPAPKPVPCGHFDGSSFIGGIVLTLGLLAIGLVAYKFYKARNERNYHTL

Target ORF information:

RefSeq Version	NM_168364.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster visgun (vsg), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168364.2

ORF Insert Sequence:

ATGAATCGGC AGGCGAAATT CCTAATCTTG TGCCTCTTTG TGGGCCTCTT CTCCGCGAAT 
TTGTGCGAAG AAGTGACCAC ACCGGCTCCA GCGGACACCA CCACCCAGGA ATCCAAGAAC 
ACCACCACTC CGCCGGACAC CACCACTACT GTGACGCCAC CGTCGACCTC AACCACGAGC 
ACCACAACTG AAAAGACCAC GACGACGCCA CCAATTACCA CATCGACTGA GAAGACCACA 
ACTTCCACTA CTCCAGCCAG CACCACCAGC AGCACCACTC CGGCCAGCAC CACCAGCAGC 
ACCACTCCGG CAACGACCAC CACCACGCCT GGAACTACAT CTACGACCAC TCCGTCTCCG 
AACTCGACCA CAACTACGCC GCCGCCACAC ACATCAACCA CTCCGGCGCC GAAGCCCGTG 
CCGTGCGGTC ATTTCGATGG ATCCTCGTTC ATTGGCGGCA TTGTGCTGAC TCTGGGCCTG 
CTCGCTATCG GCTTAGTGGC CTACAAGTTC TACAAGGCCC GCAACGAGCG CAACTACCAC 
ACCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vsg ( NM_001274717.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261646.1 Drosophila melanogaster visgun (vsg), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa20805
Clone ID Related Accession (Same CDS sequence)	NM_001274717.1
Accession Version	NM_001274717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	visgun, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCGGC AGGCGAAATT CCTAATCTTG TGCCTCTTTG TGGGCCTCTT CTCCGCGAAT 
TTGTGCGAAG AAGTGACCAC ACCGGCTCCA GCGGACACCA CCACCCAGGA ATCCAAGAAC 
ACCACCACTC CGCCGGACAC CACCACTACT GTGACGCCAC CGTCGACCTC AACCACGAGC 
ACCACAACTG AAAAGACCAC GACGACGCCA CCAATTACCA CATCGACTGA GAAGACCACA 
ACTTCCACTA CTCCAGCCAG CACCACCAGC AGCACCACTC CGGCCAGCAC CACCAGCAGC 
ACCACTCCGG CAACGACCAC CACCACGCCT GGAACTACAT CTACGACCAC TCCGTCTCCG 
AACTCGACCA CAACTACGCC GCCGCCACAC ACATCAACCA CTCCGGCGCC GAAGCCCGTG 
CCGTGCGGTC ATTTCGATGG ATCCTCGTTC ATTGGCGGCA TTGTGCTGAC TCTGGGCCTG 
CTCGCTATCG GCTTAGTGGC CTACAAGTTC TACAAGGCCC GCAACGAGCG CAACTACCAC 
ACCCTTTGAG CCGCCACCGC CTTTACGTCG GCCTTCAATG CGGCAGCCGG AGCTGCGGCG 
GCCAGCAGCA GTGCCGCATC CACGGATGCG GAGCGCGTGC GGTTTGTCCA GCCTTCGATA 
CCGCTAAGGT CGCCGCCAAC GCAGCCGCCA CCACCACCAC CCACCCACAT GGGTGGCAGT 
GGTGGTGGTG CGGCTGCAGC TCCACAAAAC TGCTCGCCGT CGGCCCGAGA TCGGTTGCTG 
CACACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261646.1
CDS	122..910
Translation	MNRQAKFLILCLFVGLFSANLCEEVTTPAPADTTTQESKNTTTPPDTTTTVTPPSTSTTSTTTEKTTTTPPITTSTEKTTTSTTPASTTSSTTPASTTSSTTPATTTTTPGTTSTTTPSPNSTTTTPPPHTSTTPAPKPVPCGHFDGSSFIGGIVLTLGLLAIGLVAYKFYKARNERNYHTL*AATAFTSAFNAAAGAAAASSSAASTDAERVRFVQPSIPLRSPPTQPPPPPPTHMGGSGGGAAAAPQNCSPSARDRLLHT

Target ORF information:

RefSeq Version	NM_001274717.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster visgun (vsg), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274717.1

ORF Insert Sequence:

ATGAATCGGC AGGCGAAATT CCTAATCTTG TGCCTCTTTG TGGGCCTCTT CTCCGCGAAT 
TTGTGCGAAG AAGTGACCAC ACCGGCTCCA GCGGACACCA CCACCCAGGA ATCCAAGAAC 
ACCACCACTC CGCCGGACAC CACCACTACT GTGACGCCAC CGTCGACCTC AACCACGAGC 
ACCACAACTG AAAAGACCAC GACGACGCCA CCAATTACCA CATCGACTGA GAAGACCACA 
ACTTCCACTA CTCCAGCCAG CACCACCAGC AGCACCACTC CGGCCAGCAC CACCAGCAGC 
ACCACTCCGG CAACGACCAC CACCACGCCT GGAACTACAT CTACGACCAC TCCGTCTCCG 
AACTCGACCA CAACTACGCC GCCGCCACAC ACATCAACCA CTCCGGCGCC GAAGCCCGTG 
CCGTGCGGTC ATTTCGATGG ATCCTCGTTC ATTGGCGGCA TTGTGCTGAC TCTGGGCCTG 
CTCGCTATCG GCTTAGTGGC CTACAAGTTC TACAAGGCCC GCAACGAGCG CAACTACCAC 
ACCCTTTGAG CCGCCACCGC CTTTACGTCG GCCTTCAATG CGGCAGCCGG AGCTGCGGCG 
GCCAGCAGCA GTGCCGCATC CACGGATGCG GAGCGCGTGC GGTTTGTCCA GCCTTCGATA 
CCGCTAAGGT CGCCGCCAAC GCAGCCGCCA CCACCACCAC CCACCCACAT GGGTGGCAGT 
GGTGGTGGTG CGGCTGCAGC TCCACAAAAC TGCTCGCCGT CGGCCCGAGA TCGGTTGCTG 
CACACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Combined evidence annotation of transposable elements in genome sequences.
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vsg cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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