Mitf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mitf
Entrez Gene ID	3885647
Full Name	CG43369 gene product from transcript CG43369-RA
Synonyms	CG17469,CG40476,CG41130,CG43369,Dmel\CG43369,Dmel_CG17469,Dmel_CG41130,MITF,MitF,TFEB,dMitf
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102F8-102F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001038719.2	NP_001033808.1	mitf, isoform A
NM_001038718.2	NP_001033807.1	mitf, isoform B
NM_001258508.2	NP_001245437.1	mitf, isoform D
NM_001258507.1	NP_001245436.1	mitf, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mitf regulates the expression of genes encoding V-ATPase subunits as well as many additional genes involved in the lysosomal-autophagy pathway.
Title: Drosophila Mitf regulates the V-ATPase and the lysosomal-autophagic pathway.

lysosomal-associated functions regulated by the TFEB-V-ATPase axis might play a conserved role in shaping cell fate.
Title: Control of lysosomal biogenesis and Notch-dependent tissue patterning by components of the TFEB-V-ATPase axis in Drosophila melanogaster.

evidence points to an ancient module comprising Mitf, v-ATPase and TORC1 that serves as a dynamic modulator of metabolism for cellular homeostasis.
Title: Mitf is a master regulator of the v-ATPase, forming a control module for cellular homeostasis with v-ATPase and TORC1.

The MITF is a transcription factor which plays a central role in the expression of melanocyte-specific genes.
Title: Cell cycle inhibitor p21/ WAF1/ CIP1 as a cofactor of MITF expression in melanoma cells.

analysis of the Mitf gene reveals novel conserved domains in human, mouse and Drosophila
Title: Evolutionary sequence comparison of the Mitf gene reveals novel conserved domains.

The following Mitf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mitf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00234	NM_001038719.2 Latest version!	Drosophila melanogaster mitf, transcript variant A (Mitf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00234	NM_001038718.2 Latest version!	Drosophila melanogaster mitf, transcript variant B (Mitf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00332	NM_001258508.2 Latest version!	Drosophila melanogaster mitf, transcript variant D (Mitf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00333	NM_001258507.1 Latest version!	Drosophila melanogaster mitf, transcript variant C (Mitf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mitf ( NM_001038719.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001033808.1 Drosophila melanogaster mitf, transcript variant A (Mitf), mRNA.

Price & Availability↑

CloneID	OFa00234
Clone ID Related Accession (Same CDS sequence)	NM_001038719.2 , NM_001038718.2
Accession Version	NM_001038719.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitf, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001038719.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAAT CTGGAATCGA TTTGGGCTTT GATATGGAGT TTGATCTAAA TATAAACTTG 
TTGAATGATA ACGACAATAT GGATTTCTTA CCAAATGTTA CTGAGAATAT GGAGTTCTAT 
GAATTGAAAT CATCATCACG CTGTATACGG CATAATGAAA TACCGACATT TAAAACTGCT 
ACTCCCACTT CACGAACACA ACTAAAACTG CAGCTACAAC GTGAACAACA GCAACAAATG 
ATGATACAAC AACAGACATT AGACACTGCT ATGGACCCAA AAATGCATCT ATTGTTTGGC 
AGTGGGCAGG GGCTTATGGA ATCGGAATTC ATTGACAGCG GTAGTACCAG TGCTTGTGGC 
AGCGGGTCAT CCAGTTTAGA ACAAATGTCA CAGTTAGTGC AAATGGACAA TTTAATTGAT 
TCGTCAAGTG GAGCCAAGCT TAAAGTTCCT CTTCAAAGTA TCGGAGTAGA TGTGCCACCC 
CAAGTGCTGC AGGTCAGTAC AGTGCTTGAA AACCCTACAA GGTATCACGT TATCCAAAAG 
CAAAAGAATC AAGTGAGGCA ATATCTCAGC GAATCCTTTA AGCCGTCTAT GTGGGGCAGT 
CATACCAGCG AAATAAAATT AGCAAATAAT TCTGCGTCAA CGGGAAATCT TCAAAATTCA 
TCTTTACAGA AAGGTATTTG CGATCCATTG GAGCGTACAA ACCGATTTGG ATGCGATTCG 
GCTGTGAGTG CTAAAAGAAT TATGCCTTCC GATGACGCAA TGCCTATTTC CCCATTCGGT 
GGTAGTTTTG TACGATGTGA TGACATAAAT CCAATTGAGC CAACTGTATT ACGACCTAAT 
AGTCATGGTG CTGGGGAACC AGAAAATGCA CATAGAACGG CCCAGTTGGG ACTTAGTAAA 
GCTAATTCAA GCCTAAGTTC AACGCGTTCT TCTTCAGGGA TTGTTAATTC AATTCGAATT 
TCCAGTACAT CAAGCTCGTT ACAGTCTACC TCAGCTCCTA TTTCGCCGAG CGTAAGTTCG 
GTTGCAACGA GTGTCTCTGA GCTACCATCA TTCGACAGCG ATCCAGATGA CATCTTCGAT 
GATATATTAC AAAATGACTC TTTTAACTTT GATAAAAACT TCAACTCTGA GCTTTCAATT 
AAACAGGAAC CTCAAAATTT AACTGATGCT GAAATGAATG CCCTAGCCAA GGACAGACAA 
AAGAAAGACA ACCATAATAT GATTGAGCGA AGGCGACGCT TTAATATAAA TGACAGAATA 
AAAGAGCTTG GTACCCTTTT GCCAAAGGGC AGTGATGCTT TTTACGAAGT AGTTCGAGAC 
ATTCGTCCAA ACAAGGGGAC AATCTTGAAG TCCTCTGTTG ACTACATTAA ATGCTTGAAA 
CATGAGGTGA CTCGCTTAAG ACAAAATGAG TTACGTCAAC GTCAAGTAGA GTTGCAAAAT 
CGAAAGCTCA TGTCTCGAAT AAAAGAATTG GAAATGCAGG CCAAGTCCCA TGGAATTTTG 
TTATCAGAAA ACCACCTAAC ATCACTATCA GCGCCTACTC AACCTTATCT TAAAAGTTTT 
AGCCTGTCTC CTACTGCTTC TCGGAGTCGT CGATCACTTT TTGACCAACC TGTTGAAAAA 
AAAATACAAG TTATTGATGG TGCCGATGGA AATATGGGAA TGAATCAGGT TGATGAGTTT 
ATGGAAGATT GCAAGTATGC AGTACAAGGC GGCGATCCGA TGTTGTCCTC GCATAGTCAC 
ATGCAATCCG CGCCACAATC ACCGAGTTCA AAAACTTTGA ATAGTGGAAG TTTTGAACCA 
AAGAATTTTT CGGAGGCTGA TGAAAGCCTT TTTAGAGGCA AATCAAGTTT AGCATCAGAT 
GACTGTTGCG GTATAAATTG CAGTACTTCG TGCTATATTC AACACCAATT GACTTCGCGT 
GAGGGCCATC CAAATCATTC TCACCACACG GGTATACGAG AAATTCATAG TTTGTCTGAT 
TCGGCTCAAA ACTCAGAGTT TAGTAGGCTT GAGCATTGCG ATGATCCACT TCTCTCATCT 
TCCCATAGGT CACTTGGCAC CGTAGACGAA GATCAACATA ATTCAGTTGA TATGTCTGCA 
GTCATGGTAC ACGACTCCCT ATCCTCTTTG GTAGATGACA ACAATAGCGA AACAATGGTT 
CTTGCATCTG ACACATTGGA TATTGAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033808.1
CDS	164..2356
Translation	MTESGIDLGFDMEFDLNINLLNDNDNMDFLPNVTENMEFYELKSSSRCIRHNEIPTFKTATPTSRTQLKLQLQREQQQQMMIQQQTLDTAMDPKMHLLFGSGQGLMESEFIDSGSTSACGSGSSSLEQMSQLVQMDNLIDSSSGAKLKVPLQSIGVDVPPQVLQVSTVLENPTRYHVIQKQKNQVRQYLSESFKPSMWGSHTSEIKLANNSASTGNLQNSSLQKGICDPLERTNRFGCDSAVSAKRIMPSDDAMPISPFGGSFVRCDDINPIEPTVLRPNSHGAGEPENAHRTAQLGLSKANSSLSSTRSSSGIVNSIRISSTSSSLQSTSAPISPSVSSVATSVSELPSFDSDPDDIFDDILQNDSFNFDKNFNSELSIKQEPQNLTDAEMNALAKDRQKKDNHNMIERRRRFNINDRIKELGTLLPKGSDAFYEVVRDIRPNKGTILKSSVDYIKCLKHEVTRLRQNELRQRQVELQNRKLMSRIKELEMQAKSHGILLSENHLTSLSAPTQPYLKSFSLSPTASRSRRSLFDQPVEKKIQVIDGADGNMGMNQVDEFMEDCKYAVQGGDPMLSSHSHMQSAPQSPSSKTLNSGSFEPKNFSEADESLFRGKSSLASDDCCGINCSTSCYIQHQLTSREGHPNHSHHTGIREIHSLSDSAQNSEFSRLEHCDDPLLSSSHRSLGTVDEDQHNSVDMSAVMVHDSLSSLVDDNNSETMVLASDTLDIEL

Target ORF information:

RefSeq Version	NM_001038719.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitf, transcript variant A (Mitf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038719.2

ORF Insert Sequence:

ATGACGGAAT CTGGAATCGA TTTGGGCTTT GATATGGAGT TTGATCTAAA TATAAACTTG 
TTGAATGATA ACGACAATAT GGATTTCTTA CCAAATGTTA CTGAGAATAT GGAGTTCTAT 
GAATTGAAAT CATCATCACG CTGTATACGG CATAATGAAA TACCGACATT TAAAACTGCT 
ACTCCCACTT CACGAACACA ACTAAAACTG CAGCTACAAC GTGAACAACA GCAACAAATG 
ATGATACAAC AACAGACATT AGACACTGCT ATGGACCCAA AAATGCATCT ATTGTTTGGC 
AGTGGGCAGG GGCTTATGGA ATCGGAATTC ATTGACAGCG GTAGTACCAG TGCTTGTGGC 
AGCGGGTCAT CCAGTTTAGA ACAAATGTCA CAGTTAGTGC AAATGGACAA TTTAATTGAT 
TCGTCAAGTG GAGCCAAGCT TAAAGTTCCT CTTCAAAGTA TCGGAGTAGA TGTGCCACCC 
CAAGTGCTGC AGGTCAGTAC AGTGCTTGAA AACCCTACAA GGTATCACGT TATCCAAAAG 
CAAAAGAATC AAGTGAGGCA ATATCTCAGC GAATCCTTTA AGCCGTCTAT GTGGGGCAGT 
CATACCAGCG AAATAAAATT AGCAAATAAT TCTGCGTCAA CGGGAAATCT TCAAAATTCA 
TCTTTACAGA AAGGTATTTG CGATCCATTG GAGCGTACAA ACCGATTTGG ATGCGATTCG 
GCTGTGAGTG CTAAAAGAAT TATGCCTTCC GATGACGCAA TGCCTATTTC CCCATTCGGT 
GGTAGTTTTG TACGATGTGA TGACATAAAT CCAATTGAGC CAACTGTATT ACGACCTAAT 
AGTCATGGTG CTGGGGAACC AGAAAATGCA CATAGAACGG CCCAGTTGGG ACTTAGTAAA 
GCTAATTCAA GCCTAAGTTC AACGCGTTCT TCTTCAGGGA TTGTTAATTC AATTCGAATT 
TCCAGTACAT CAAGCTCGTT ACAGTCTACC TCAGCTCCTA TTTCGCCGAG CGTAAGTTCG 
GTTGCAACGA GTGTCTCTGA GCTACCATCA TTCGACAGCG ATCCAGATGA CATCTTCGAT 
GATATATTAC AAAATGACTC TTTTAACTTT GATAAAAACT TCAACTCTGA GCTTTCAATT 
AAACAGGAAC CTCAAAATTT AACTGATGCT GAAATGAATG CCCTAGCCAA GGACAGACAA 
AAGAAAGACA ACCATAATAT GATTGAGCGA AGGCGACGCT TTAATATAAA TGACAGAATA 
AAAGAGCTTG GTACCCTTTT GCCAAAGGGC AGTGATGCTT TTTACGAAGT AGTTCGAGAC 
ATTCGTCCAA ACAAGGGGAC AATCTTGAAG TCCTCTGTTG ACTACATTAA ATGCTTGAAA 
CATGAGGTGA CTCGCTTAAG ACAAAATGAG TTACGTCAAC GTCAAGTAGA GTTGCAAAAT 
CGAAAGCTCA TGTCTCGAAT AAAAGAATTG GAAATGCAGG CCAAGTCCCA TGGAATTTTG 
TTATCAGAAA ACCACCTAAC ATCACTATCA GCGCCTACTC AACCTTATCT TAAAAGTTTT 
AGCCTGTCTC CTACTGCTTC TCGGAGTCGT CGATCACTTT TTGACCAACC TGTTGAAAAA 
AAAATACAAG TTATTGATGG TGCCGATGGA AATATGGGAA TGAATCAGGT TGATGAGTTT 
ATGGAAGATT GCAAGTATGC AGTACAAGGC GGCGATCCGA TGTTGTCCTC GCATAGTCAC 
ATGCAATCCG CGCCACAATC ACCGAGTTCA AAAACTTTGA ATAGTGGAAG TTTTGAACCA 
AAGAATTTTT CGGAGGCTGA TGAAAGCCTT TTTAGAGGCA AATCAAGTTT AGCATCAGAT 
GACTGTTGCG GTATAAATTG CAGTACTTCG TGCTATATTC AACACCAATT GACTTCGCGT 
GAGGGCCATC CAAATCATTC TCACCACACG GGTATACGAG AAATTCATAG TTTGTCTGAT 
TCGGCTCAAA ACTCAGAGTT TAGTAGGCTT GAGCATTGCG ATGATCCACT TCTCTCATCT 
TCCCATAGGT CACTTGGCAC CGTAGACGAA GATCAACATA ATTCAGTTGA TATGTCTGCA 
GTCATGGTAC ACGACTCCCT ATCCTCTTTG GTAGATGACA ACAATAGCGA AACAATGGTT 
CTTGCATCTG ACACATTGGA TATTGAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mitf ( NM_001038718.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001033807.1 Drosophila melanogaster mitf, transcript variant B (Mitf), mRNA.

Price & Availability↑

CloneID	OFa00234
Clone ID Related Accession (Same CDS sequence)	NM_001038719.2 , NM_001038718.2
Accession Version	NM_001038718.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitf, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001038718.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAAT CTGGAATCGA TTTGGGCTTT GATATGGAGT TTGATCTAAA TATAAACTTG 
TTGAATGATA ACGACAATAT GGATTTCTTA CCAAATGTTA CTGAGAATAT GGAGTTCTAT 
GAATTGAAAT CATCATCACG CTGTATACGG CATAATGAAA TACCGACATT TAAAACTGCT 
ACTCCCACTT CACGAACACA ACTAAAACTG CAGCTACAAC GTGAACAACA GCAACAAATG 
ATGATACAAC AACAGACATT AGACACTGCT ATGGACCCAA AAATGCATCT ATTGTTTGGC 
AGTGGGCAGG GGCTTATGGA ATCGGAATTC ATTGACAGCG GTAGTACCAG TGCTTGTGGC 
AGCGGGTCAT CCAGTTTAGA ACAAATGTCA CAGTTAGTGC AAATGGACAA TTTAATTGAT 
TCGTCAAGTG GAGCCAAGCT TAAAGTTCCT CTTCAAAGTA TCGGAGTAGA TGTGCCACCC 
CAAGTGCTGC AGGTCAGTAC AGTGCTTGAA AACCCTACAA GGTATCACGT TATCCAAAAG 
CAAAAGAATC AAGTGAGGCA ATATCTCAGC GAATCCTTTA AGCCGTCTAT GTGGGGCAGT 
CATACCAGCG AAATAAAATT AGCAAATAAT TCTGCGTCAA CGGGAAATCT TCAAAATTCA 
TCTTTACAGA AAGGTATTTG CGATCCATTG GAGCGTACAA ACCGATTTGG ATGCGATTCG 
GCTGTGAGTG CTAAAAGAAT TATGCCTTCC GATGACGCAA TGCCTATTTC CCCATTCGGT 
GGTAGTTTTG TACGATGTGA TGACATAAAT CCAATTGAGC CAACTGTATT ACGACCTAAT 
AGTCATGGTG CTGGGGAACC AGAAAATGCA CATAGAACGG CCCAGTTGGG ACTTAGTAAA 
GCTAATTCAA GCCTAAGTTC AACGCGTTCT TCTTCAGGGA TTGTTAATTC AATTCGAATT 
TCCAGTACAT CAAGCTCGTT ACAGTCTACC TCAGCTCCTA TTTCGCCGAG CGTAAGTTCG 
GTTGCAACGA GTGTCTCTGA GCTACCATCA TTCGACAGCG ATCCAGATGA CATCTTCGAT 
GATATATTAC AAAATGACTC TTTTAACTTT GATAAAAACT TCAACTCTGA GCTTTCAATT 
AAACAGGAAC CTCAAAATTT AACTGATGCT GAAATGAATG CCCTAGCCAA GGACAGACAA 
AAGAAAGACA ACCATAATAT GATTGAGCGA AGGCGACGCT TTAATATAAA TGACAGAATA 
AAAGAGCTTG GTACCCTTTT GCCAAAGGGC AGTGATGCTT TTTACGAAGT AGTTCGAGAC 
ATTCGTCCAA ACAAGGGGAC AATCTTGAAG TCCTCTGTTG ACTACATTAA ATGCTTGAAA 
CATGAGGTGA CTCGCTTAAG ACAAAATGAG TTACGTCAAC GTCAAGTAGA GTTGCAAAAT 
CGAAAGCTCA TGTCTCGAAT AAAAGAATTG GAAATGCAGG CCAAGTCCCA TGGAATTTTG 
TTATCAGAAA ACCACCTAAC ATCACTATCA GCGCCTACTC AACCTTATCT TAAAAGTTTT 
AGCCTGTCTC CTACTGCTTC TCGGAGTCGT CGATCACTTT TTGACCAACC TGTTGAAAAA 
AAAATACAAG TTATTGATGG TGCCGATGGA AATATGGGAA TGAATCAGGT TGATGAGTTT 
ATGGAAGATT GCAAGTATGC AGTACAAGGC GGCGATCCGA TGTTGTCCTC GCATAGTCAC 
ATGCAATCCG CGCCACAATC ACCGAGTTCA AAAACTTTGA ATAGTGGAAG TTTTGAACCA 
AAGAATTTTT CGGAGGCTGA TGAAAGCCTT TTTAGAGGCA AATCAAGTTT AGCATCAGAT 
GACTGTTGCG GTATAAATTG CAGTACTTCG TGCTATATTC AACACCAATT GACTTCGCGT 
GAGGGCCATC CAAATCATTC TCACCACACG GGTATACGAG AAATTCATAG TTTGTCTGAT 
TCGGCTCAAA ACTCAGAGTT TAGTAGGCTT GAGCATTGCG ATGATCCACT TCTCTCATCT 
TCCCATAGGT CACTTGGCAC CGTAGACGAA GATCAACATA ATTCAGTTGA TATGTCTGCA 
GTCATGGTAC ACGACTCCCT ATCCTCTTTG GTAGATGACA ACAATAGCGA AACAATGGTT 
CTTGCATCTG ACACATTGGA TATTGAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033807.1
CDS	158..2350
Translation	MTESGIDLGFDMEFDLNINLLNDNDNMDFLPNVTENMEFYELKSSSRCIRHNEIPTFKTATPTSRTQLKLQLQREQQQQMMIQQQTLDTAMDPKMHLLFGSGQGLMESEFIDSGSTSACGSGSSSLEQMSQLVQMDNLIDSSSGAKLKVPLQSIGVDVPPQVLQVSTVLENPTRYHVIQKQKNQVRQYLSESFKPSMWGSHTSEIKLANNSASTGNLQNSSLQKGICDPLERTNRFGCDSAVSAKRIMPSDDAMPISPFGGSFVRCDDINPIEPTVLRPNSHGAGEPENAHRTAQLGLSKANSSLSSTRSSSGIVNSIRISSTSSSLQSTSAPISPSVSSVATSVSELPSFDSDPDDIFDDILQNDSFNFDKNFNSELSIKQEPQNLTDAEMNALAKDRQKKDNHNMIERRRRFNINDRIKELGTLLPKGSDAFYEVVRDIRPNKGTILKSSVDYIKCLKHEVTRLRQNELRQRQVELQNRKLMSRIKELEMQAKSHGILLSENHLTSLSAPTQPYLKSFSLSPTASRSRRSLFDQPVEKKIQVIDGADGNMGMNQVDEFMEDCKYAVQGGDPMLSSHSHMQSAPQSPSSKTLNSGSFEPKNFSEADESLFRGKSSLASDDCCGINCSTSCYIQHQLTSREGHPNHSHHTGIREIHSLSDSAQNSEFSRLEHCDDPLLSSSHRSLGTVDEDQHNSVDMSAVMVHDSLSSLVDDNNSETMVLASDTLDIEL

Target ORF information:

RefSeq Version	NM_001038718.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitf, transcript variant B (Mitf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038718.2

ORF Insert Sequence:

ATGACGGAAT CTGGAATCGA TTTGGGCTTT GATATGGAGT TTGATCTAAA TATAAACTTG 
TTGAATGATA ACGACAATAT GGATTTCTTA CCAAATGTTA CTGAGAATAT GGAGTTCTAT 
GAATTGAAAT CATCATCACG CTGTATACGG CATAATGAAA TACCGACATT TAAAACTGCT 
ACTCCCACTT CACGAACACA ACTAAAACTG CAGCTACAAC GTGAACAACA GCAACAAATG 
ATGATACAAC AACAGACATT AGACACTGCT ATGGACCCAA AAATGCATCT ATTGTTTGGC 
AGTGGGCAGG GGCTTATGGA ATCGGAATTC ATTGACAGCG GTAGTACCAG TGCTTGTGGC 
AGCGGGTCAT CCAGTTTAGA ACAAATGTCA CAGTTAGTGC AAATGGACAA TTTAATTGAT 
TCGTCAAGTG GAGCCAAGCT TAAAGTTCCT CTTCAAAGTA TCGGAGTAGA TGTGCCACCC 
CAAGTGCTGC AGGTCAGTAC AGTGCTTGAA AACCCTACAA GGTATCACGT TATCCAAAAG 
CAAAAGAATC AAGTGAGGCA ATATCTCAGC GAATCCTTTA AGCCGTCTAT GTGGGGCAGT 
CATACCAGCG AAATAAAATT AGCAAATAAT TCTGCGTCAA CGGGAAATCT TCAAAATTCA 
TCTTTACAGA AAGGTATTTG CGATCCATTG GAGCGTACAA ACCGATTTGG ATGCGATTCG 
GCTGTGAGTG CTAAAAGAAT TATGCCTTCC GATGACGCAA TGCCTATTTC CCCATTCGGT 
GGTAGTTTTG TACGATGTGA TGACATAAAT CCAATTGAGC CAACTGTATT ACGACCTAAT 
AGTCATGGTG CTGGGGAACC AGAAAATGCA CATAGAACGG CCCAGTTGGG ACTTAGTAAA 
GCTAATTCAA GCCTAAGTTC AACGCGTTCT TCTTCAGGGA TTGTTAATTC AATTCGAATT 
TCCAGTACAT CAAGCTCGTT ACAGTCTACC TCAGCTCCTA TTTCGCCGAG CGTAAGTTCG 
GTTGCAACGA GTGTCTCTGA GCTACCATCA TTCGACAGCG ATCCAGATGA CATCTTCGAT 
GATATATTAC AAAATGACTC TTTTAACTTT GATAAAAACT TCAACTCTGA GCTTTCAATT 
AAACAGGAAC CTCAAAATTT AACTGATGCT GAAATGAATG CCCTAGCCAA GGACAGACAA 
AAGAAAGACA ACCATAATAT GATTGAGCGA AGGCGACGCT TTAATATAAA TGACAGAATA 
AAAGAGCTTG GTACCCTTTT GCCAAAGGGC AGTGATGCTT TTTACGAAGT AGTTCGAGAC 
ATTCGTCCAA ACAAGGGGAC AATCTTGAAG TCCTCTGTTG ACTACATTAA ATGCTTGAAA 
CATGAGGTGA CTCGCTTAAG ACAAAATGAG TTACGTCAAC GTCAAGTAGA GTTGCAAAAT 
CGAAAGCTCA TGTCTCGAAT AAAAGAATTG GAAATGCAGG CCAAGTCCCA TGGAATTTTG 
TTATCAGAAA ACCACCTAAC ATCACTATCA GCGCCTACTC AACCTTATCT TAAAAGTTTT 
AGCCTGTCTC CTACTGCTTC TCGGAGTCGT CGATCACTTT TTGACCAACC TGTTGAAAAA 
AAAATACAAG TTATTGATGG TGCCGATGGA AATATGGGAA TGAATCAGGT TGATGAGTTT 
ATGGAAGATT GCAAGTATGC AGTACAAGGC GGCGATCCGA TGTTGTCCTC GCATAGTCAC 
ATGCAATCCG CGCCACAATC ACCGAGTTCA AAAACTTTGA ATAGTGGAAG TTTTGAACCA 
AAGAATTTTT CGGAGGCTGA TGAAAGCCTT TTTAGAGGCA AATCAAGTTT AGCATCAGAT 
GACTGTTGCG GTATAAATTG CAGTACTTCG TGCTATATTC AACACCAATT GACTTCGCGT 
GAGGGCCATC CAAATCATTC TCACCACACG GGTATACGAG AAATTCATAG TTTGTCTGAT 
TCGGCTCAAA ACTCAGAGTT TAGTAGGCTT GAGCATTGCG ATGATCCACT TCTCTCATCT 
TCCCATAGGT CACTTGGCAC CGTAGACGAA GATCAACATA ATTCAGTTGA TATGTCTGCA 
GTCATGGTAC ACGACTCCCT ATCCTCTTTG GTAGATGACA ACAATAGCGA AACAATGGTT 
CTTGCATCTG ACACATTGGA TATTGAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mitf ( NM_001258508.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245437.1 Drosophila melanogaster mitf, transcript variant D (Mitf), mRNA.

Price & Availability↑

CloneID	OFa00332
Clone ID Related Accession (Same CDS sequence)	NM_001258508.2
Accession Version	NM_001258508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitf, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001258508.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAAT CTGGAATCGA TTTGGGCTTT GATATGGAGT TTGATCTAAA TATAAACTTG 
TTGAATGATA ACGACAATAT GGATTTCTTA CCAAATGTTA CTGAGAATAT GGAGTTCTAT 
GAATTGAAAT CATCATCACG CTGTATACGG CATAATGAAA TACCGACATT TAAAACTGCT 
ACTCCCACTT CACGAACACA ACTAAAACTG CAGCTACAAC GTGAACAACA GCAACAAATG 
ATGATACAAC AACAGACATT AGACACTGCT ATGGACCCAA AAATGCATCT ATTGTTTGGC 
AGTGGGCAGG GGCTTATGGA ATCGGAATTC ATTGACAGCG GTAGTACCAG TGCTTGTGGC 
AGCGGGTCAT CCAGTTTAGA ACAAATGTCA CAGTTAGTGC AAATGGACAA TTTAATTGAT 
TCGTCAAGTG GAGCCAAGCT TAAAGTTCCT CTTCAAAGTA TCGGAGTAGA TGTGCCACCC 
CAAGTGCTGC AGGTCAGTAC AGTGCTTGAA AACCCTACAA GGTATCACGT TATCCAAAAG 
CAAAAGAATC AAGTGAGGCA ATATCTCAGC GAATCCTTTA AGCCGTCTAT GTGGGGCAGT 
CATACCAGCG AAAAAGGTAT TTGCGATCCA TTGGAGCGTA CAAACCGATT TGGATGCGAT 
TCGGCTGTGA GTGCTAAAAG AATTATGCCT TCCGATGACG CAATGCCTAT TTCCCCATTC 
GGTGGTAGTT TTGTACGATG TGATGACATA AATCCAATTG AGCCAACTGT ATTACGACCT 
AATAGTCATG GTGCTGGGGA ACCAGAAAAT GCACATAGAA CGGCCCAGTT GGGACTTAGT 
AAAGCTAATT CAAGCCTAAG TTCAACGCGT TCTTCTTCAG GGATTGTTAA TTCAATTCGA 
ATTTCCAGTA CATCAAGCTC GTTACAGTCT ACCTCAGCTC CTATTTCGCC GAGCGTAAGT 
TCGGTTGCAA CGAGTGTCTC TGAGCCAGAT GACATCTTCG ATGATATATT ACAAAATGAC 
TCTTTTAACT TTGATAAAAA CTTCAACTCT GAGCTTTCAA TTAAACAGGA ACCTCAAAAT 
TTAACTGATG CTGAAATGAA TGCCCTAGCC AAGGACAGAC AAAAGAAAGA CAACCATAAT 
ATGATTGAGC GAAGGCGACG CTTTAATATA AATGACAGAA TAAAAGAGCT TGGTACCCTT 
TTGCCAAAGG GCAGTGATGC TTTTTACGAA GTAGTTCGAG ACATTCGTCC AAACAAGGGG 
ACAATCTTGA AGTCCTCTGT TGACTACATT AAATGCTTGA AACATGAGGT GACTCGCTTA 
AGACAAAATG AGTTACGTCA ACGTCAAGTA GAGTTGCAAA ATCGAAAGCT CATGTCTCGA 
ATAAAAGAAT TGGAAATGCA GGCCAAGTCC CATGGAATTT TGTTATCAGA AAACCACCTA 
ACATCACTAT CAGCGCCTAC TCAACCTTAT CTTAAAAGTT TTAGCCTGTC TCCTACTGCT 
TCTCGGAGTC GTCGATCACT TTTTGACCAA CCTGTTGAAA AAAAAATACA AGTTATTGAT 
GGTGCCGATG GAAATATGGG AATGAATCAG GTTGATGAGT TTATGGAAGA TTGCAAGTAT 
GCAGTACAAG GCGGCGATCC GATGTTGTCC TCGCATAGTC ACATGCAATC CGCGCCACAA 
TCACCGAGTT CAAAAACTTT GAATAGTGGA AGTTTTGAAC CAAAGAATTT TTCGGAGGCT 
GATGAAAGCC TTTTTAGAGG CAAATCAAGT TTAGCATCAG ATGACTGTTG CGGTATAAAT 
TGCAGTACTT CGTGCTATAT TCAACACCAA TTGACTTCGC GTGAGGGCCA TCCAAATCAT 
TCTCACCACA CGGGTATACG AGAAATTCAT AGTTTGTCTG ATTCGGCTCA AAACTCAGAG 
TTTAGTAGGC TTGAGCATTG CGATGATCCA CTTCTCTCAT CTTCCCATAG GTCACTTGGC 
ACCGTAGACG AAGATCAACA TAATTCAGTT GATATGTCTG CAGTCATGGT ACACGACTCC 
CTATCCTCTT TGGTAGATGA CAACAATAGC GAAACAATGG TTCTTGCATC TGACACATTG 
GATATTGAGT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245437.1
CDS	158..2272
Translation	MTESGIDLGFDMEFDLNINLLNDNDNMDFLPNVTENMEFYELKSSSRCIRHNEIPTFKTATPTSRTQLKLQLQREQQQQMMIQQQTLDTAMDPKMHLLFGSGQGLMESEFIDSGSTSACGSGSSSLEQMSQLVQMDNLIDSSSGAKLKVPLQSIGVDVPPQVLQVSTVLENPTRYHVIQKQKNQVRQYLSESFKPSMWGSHTSEKGICDPLERTNRFGCDSAVSAKRIMPSDDAMPISPFGGSFVRCDDINPIEPTVLRPNSHGAGEPENAHRTAQLGLSKANSSLSSTRSSSGIVNSIRISSTSSSLQSTSAPISPSVSSVATSVSEPDDIFDDILQNDSFNFDKNFNSELSIKQEPQNLTDAEMNALAKDRQKKDNHNMIERRRRFNINDRIKELGTLLPKGSDAFYEVVRDIRPNKGTILKSSVDYIKCLKHEVTRLRQNELRQRQVELQNRKLMSRIKELEMQAKSHGILLSENHLTSLSAPTQPYLKSFSLSPTASRSRRSLFDQPVEKKIQVIDGADGNMGMNQVDEFMEDCKYAVQGGDPMLSSHSHMQSAPQSPSSKTLNSGSFEPKNFSEADESLFRGKSSLASDDCCGINCSTSCYIQHQLTSREGHPNHSHHTGIREIHSLSDSAQNSEFSRLEHCDDPLLSSSHRSLGTVDEDQHNSVDMSAVMVHDSLSSLVDDNNSETMVLASDTLDIEL

Target ORF information:

RefSeq Version	NM_001258508.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitf, transcript variant D (Mitf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258508.2

ORF Insert Sequence:

ATGACGGAAT CTGGAATCGA TTTGGGCTTT GATATGGAGT TTGATCTAAA TATAAACTTG 
TTGAATGATA ACGACAATAT GGATTTCTTA CCAAATGTTA CTGAGAATAT GGAGTTCTAT 
GAATTGAAAT CATCATCACG CTGTATACGG CATAATGAAA TACCGACATT TAAAACTGCT 
ACTCCCACTT CACGAACACA ACTAAAACTG CAGCTACAAC GTGAACAACA GCAACAAATG 
ATGATACAAC AACAGACATT AGACACTGCT ATGGACCCAA AAATGCATCT ATTGTTTGGC 
AGTGGGCAGG GGCTTATGGA ATCGGAATTC ATTGACAGCG GTAGTACCAG TGCTTGTGGC 
AGCGGGTCAT CCAGTTTAGA ACAAATGTCA CAGTTAGTGC AAATGGACAA TTTAATTGAT 
TCGTCAAGTG GAGCCAAGCT TAAAGTTCCT CTTCAAAGTA TCGGAGTAGA TGTGCCACCC 
CAAGTGCTGC AGGTCAGTAC AGTGCTTGAA AACCCTACAA GGTATCACGT TATCCAAAAG 
CAAAAGAATC AAGTGAGGCA ATATCTCAGC GAATCCTTTA AGCCGTCTAT GTGGGGCAGT 
CATACCAGCG AAAAAGGTAT TTGCGATCCA TTGGAGCGTA CAAACCGATT TGGATGCGAT 
TCGGCTGTGA GTGCTAAAAG AATTATGCCT TCCGATGACG CAATGCCTAT TTCCCCATTC 
GGTGGTAGTT TTGTACGATG TGATGACATA AATCCAATTG AGCCAACTGT ATTACGACCT 
AATAGTCATG GTGCTGGGGA ACCAGAAAAT GCACATAGAA CGGCCCAGTT GGGACTTAGT 
AAAGCTAATT CAAGCCTAAG TTCAACGCGT TCTTCTTCAG GGATTGTTAA TTCAATTCGA 
ATTTCCAGTA CATCAAGCTC GTTACAGTCT ACCTCAGCTC CTATTTCGCC GAGCGTAAGT 
TCGGTTGCAA CGAGTGTCTC TGAGCCAGAT GACATCTTCG ATGATATATT ACAAAATGAC 
TCTTTTAACT TTGATAAAAA CTTCAACTCT GAGCTTTCAA TTAAACAGGA ACCTCAAAAT 
TTAACTGATG CTGAAATGAA TGCCCTAGCC AAGGACAGAC AAAAGAAAGA CAACCATAAT 
ATGATTGAGC GAAGGCGACG CTTTAATATA AATGACAGAA TAAAAGAGCT TGGTACCCTT 
TTGCCAAAGG GCAGTGATGC TTTTTACGAA GTAGTTCGAG ACATTCGTCC AAACAAGGGG 
ACAATCTTGA AGTCCTCTGT TGACTACATT AAATGCTTGA AACATGAGGT GACTCGCTTA 
AGACAAAATG AGTTACGTCA ACGTCAAGTA GAGTTGCAAA ATCGAAAGCT CATGTCTCGA 
ATAAAAGAAT TGGAAATGCA GGCCAAGTCC CATGGAATTT TGTTATCAGA AAACCACCTA 
ACATCACTAT CAGCGCCTAC TCAACCTTAT CTTAAAAGTT TTAGCCTGTC TCCTACTGCT 
TCTCGGAGTC GTCGATCACT TTTTGACCAA CCTGTTGAAA AAAAAATACA AGTTATTGAT 
GGTGCCGATG GAAATATGGG AATGAATCAG GTTGATGAGT TTATGGAAGA TTGCAAGTAT 
GCAGTACAAG GCGGCGATCC GATGTTGTCC TCGCATAGTC ACATGCAATC CGCGCCACAA 
TCACCGAGTT CAAAAACTTT GAATAGTGGA AGTTTTGAAC CAAAGAATTT TTCGGAGGCT 
GATGAAAGCC TTTTTAGAGG CAAATCAAGT TTAGCATCAG ATGACTGTTG CGGTATAAAT 
TGCAGTACTT CGTGCTATAT TCAACACCAA TTGACTTCGC GTGAGGGCCA TCCAAATCAT 
TCTCACCACA CGGGTATACG AGAAATTCAT AGTTTGTCTG ATTCGGCTCA AAACTCAGAG 
TTTAGTAGGC TTGAGCATTG CGATGATCCA CTTCTCTCAT CTTCCCATAG GTCACTTGGC 
ACCGTAGACG AAGATCAACA TAATTCAGTT GATATGTCTG CAGTCATGGT ACACGACTCC 
CTATCCTCTT TGGTAGATGA CAACAATAGC GAAACAATGG TTCTTGCATC TGACACATTG 
GATATTGAGT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mitf ( NM_001258507.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245436.1 Drosophila melanogaster mitf, transcript variant C (Mitf), mRNA.

Price & Availability↑

CloneID	OFa00333
Clone ID Related Accession (Same CDS sequence)	NM_001258507.1
Accession Version	NM_001258507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitf, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTTCTT ACTGGACCAA ACAGAATATA TCAAGCTCAG TAGATGGAAA AAAGATGGAC 
AAAGTTTCTA CCATGAGAAA AAAAAATTCA ACAAAAGTTA AAATGGAGAA AAAAGAAACA 
GTTGTACCGA TTACAGAAAT GCGTGCTCTG GAATTTCAAG ATTTCCTTAA GCGGGTACAG 
ACGTTACAAA TGTCGCACAA CGAAAGCCGC GATTTAGTCA ATGGACCTAA TATCTGCAGT 
TCCATGGATA TTGAAGTCGG CGAAGAACCT CCTACGACAT CTTTTGCCGA TAACATAGAT 
AAAGCGTGTC CTTTACCAGT GCTGCGATCA TCTGATTGTG ATTGTGTGCC ACTTTGTATG 
ACAAATATTC GTGTATCAGT TGGTATTGAC AAAGACTTAG AAATGATTCT TGAAATGGAT 
CCGAGTATTG TAGATTTGGG TGATATTAGC ATTGATGAAA CTGCAGAACC GCGCATAGTG 
GGCTTACCTC CACTTTCGGG TGGACCGACA TTTAAAACTG CTACTCCCAC TTCACGAACA 
CAACTAAAAC TGCAGCTACA ACGTGAACAA CAGCAACAAA TGATGATACA ACAACAGACA 
TTAGACACTG CTATGGACCC AAAAATGCAT CTATTGTTTG GCAGTGGGCA GGGGCTTATG 
GAATCGGAAT TCATTGACAG CGGTAGTACC AGTGCTTGTG GCAGCGGGTC ATCCAGTTTA 
GAACAAATGT CACAGTTAGT GCAAATGGAC AATTTAATTG ATTCGTCAAG TGGAGCCAAG 
CTTAAAGTTC CTCTTCAAAG TATCGGAGTA GATGTGCCAC CCCAAGTGCT GCAGGTCAGT 
ACAGTGCTTG AAAACCCTAC AAGGTATCAC GTTATCCAAA AGCAAAAGAA TCAAGTGAGG 
CAATATCTCA GCGAATCCTT TAAGCCGTCT ATGTGGGGCA GTCATACCAG CGAAATAAAA 
TTAGCAAATA ATTCTGCGTC AACGGGAAAT CTTCAAAATT CATCTTTACA GAAAGGTATT 
TGCGATCCAT TGGAGCGTAC AAACCGATTT GGATGCGATT CGGCTGTGAG TGCTAAAAGA 
ATTATGCCTT CCGATGACGC AATGCCTATT TCCCCATTCG GTGGTAGTTT TGTACGATGT 
GATGACATAA ATCCAATTGA GCCAACTGTA TTACGACCTA ATAGTCATGG TGCTGGGGAA 
CCAGAAAATG CACATAGAAC GGCCCAGTTG GGACTTAGTA AAGCTAATTC AAGCCTAAGT 
TCAACGCGTT CTTCTTCAGG GATTGTTAAT TCAATTCGAA TTTCCAGTAC ATCAAGCTCG 
TTACAGTCTA CCTCAGCTCC TATTTCGCCG AGCGTAAGTT CGGTTGCAAC GAGTGTCTCT 
GAGCCAGATG ACATCTTCGA TGATATATTA CAAAATGACT CTTTTAACTT TGATAAAAAC 
TTCAACTCTG AGCTTTCAAT TAAACAGGAA CCTCAAAATT TAACTGATGC TGAAATGAAT 
GCCCTAGCCA AGGACAGACA AAAGAAAGAC AACCATAATA TGATTGAGCG AAGGCGACGC 
TTTAATATAA ATGACAGAAT AAAAGAGCTT GGTACCCTTT TGCCAAAGGG CAGTGATGCT 
TTTTACGAAG TAGTTCGAGA CATTCGTCCA AACAAGGGGA CAATCTTGAA GTCCTCTGTT 
GACTACATTA AATGCTTGAA ACATGAGGTG ACTCGCTTAA GACAAAATGA GTTACGTCAA 
CGTCAAGTAG AGTTGCAAAA TCGAAAGCTC ATGTCTCGAA TAAAAGAATT GGAAATGCAG 
GCCAAGTCCC ATGGAATTTT GTTATCAGAA AACCACCTAA CATCACTATC AGCGCCTACT 
CAACCTTATC TTAAAAGTTT TAGCCTGTCT CCTACTGCTT CTCGGAGTCG TCGATCACTT 
TTTGACCAAC CTGTTGAAAA AAAAATACAA GTTATTGATG GTGCCGATGG AAATATGGGA 
ATGAATCAGG TTGATGAGTT TATGGAAGAT TGCAAGTATG CAGTACAAGG CGGCGATCCG 
ATGTTGTCCT CGCATAGTCA CATGCAATCC GCGCCACAAT CACCGAGTTC AAAAACTTTG 
AATAGTGGAA GTTTTGAACC AAAGAATTTT TCGGAGGCTG ATGAAAGCCT TTTTAGAGGC 
AAATCAAGTT TAGCATCAGA TGACTGTTGC GGTATAAATT GCAGTACTTC GTGCTATATT 
CAACACCAAT TGACTTCGCG TGAGGGCCAT CCAAATCATT CTCACCACAC GGGTATACGA 
GAAATTCATA GTTTGTCTGA TTCGGCTCAA AACTCAGAGT TTAGTAGGCT TGAGCATTGC 
GATGATCCAC TTCTCTCATC TTCCCATAGG TCACTTGGCA CCGTAGACGA AGATCAACAT 
AATTCAGTTG ATATGTCTGC AGTCATGGTA CACGACTCCC TATCCTCTTT GGTAGATGAC 
AACAATAGCG AAACAATGGT TCTTGCATCT GACACATTGG ATATTGAGTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245436.1
CDS	54..2567
Translation	MFSYWTKQNISSSVDGKKMDKVSTMRKKNSTKVKMEKKETVVPITEMRALEFQDFLKRVQTLQMSHNESRDLVNGPNICSSMDIEVGEEPPTTSFADNIDKACPLPVLRSSDCDCVPLCMTNIRVSVGIDKDLEMILEMDPSIVDLGDISIDETAEPRIVGLPPLSGGPTFKTATPTSRTQLKLQLQREQQQQMMIQQQTLDTAMDPKMHLLFGSGQGLMESEFIDSGSTSACGSGSSSLEQMSQLVQMDNLIDSSSGAKLKVPLQSIGVDVPPQVLQVSTVLENPTRYHVIQKQKNQVRQYLSESFKPSMWGSHTSEIKLANNSASTGNLQNSSLQKGICDPLERTNRFGCDSAVSAKRIMPSDDAMPISPFGGSFVRCDDINPIEPTVLRPNSHGAGEPENAHRTAQLGLSKANSSLSSTRSSSGIVNSIRISSTSSSLQSTSAPISPSVSSVATSVSEPDDIFDDILQNDSFNFDKNFNSELSIKQEPQNLTDAEMNALAKDRQKKDNHNMIERRRRFNINDRIKELGTLLPKGSDAFYEVVRDIRPNKGTILKSSVDYIKCLKHEVTRLRQNELRQRQVELQNRKLMSRIKELEMQAKSHGILLSENHLTSLSAPTQPYLKSFSLSPTASRSRRSLFDQPVEKKIQVIDGADGNMGMNQVDEFMEDCKYAVQGGDPMLSSHSHMQSAPQSPSSKTLNSGSFEPKNFSEADESLFRGKSSLASDDCCGINCSTSCYIQHQLTSREGHPNHSHHTGIREIHSLSDSAQNSEFSRLEHCDDPLLSSSHRSLGTVDEDQHNSVDMSAVMVHDSLSSLVDDNNSETMVLASDTLDIEL

Target ORF information:

RefSeq Version	NM_001258507.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitf, transcript variant C (Mitf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258507.1

ORF Insert Sequence:

ATGTTTTCTT ACTGGACCAA ACAGAATATA TCAAGCTCAG TAGATGGAAA AAAGATGGAC 
AAAGTTTCTA CCATGAGAAA AAAAAATTCA ACAAAAGTTA AAATGGAGAA AAAAGAAACA 
GTTGTACCGA TTACAGAAAT GCGTGCTCTG GAATTTCAAG ATTTCCTTAA GCGGGTACAG 
ACGTTACAAA TGTCGCACAA CGAAAGCCGC GATTTAGTCA ATGGACCTAA TATCTGCAGT 
TCCATGGATA TTGAAGTCGG CGAAGAACCT CCTACGACAT CTTTTGCCGA TAACATAGAT 
AAAGCGTGTC CTTTACCAGT GCTGCGATCA TCTGATTGTG ATTGTGTGCC ACTTTGTATG 
ACAAATATTC GTGTATCAGT TGGTATTGAC AAAGACTTAG AAATGATTCT TGAAATGGAT 
CCGAGTATTG TAGATTTGGG TGATATTAGC ATTGATGAAA CTGCAGAACC GCGCATAGTG 
GGCTTACCTC CACTTTCGGG TGGACCGACA TTTAAAACTG CTACTCCCAC TTCACGAACA 
CAACTAAAAC TGCAGCTACA ACGTGAACAA CAGCAACAAA TGATGATACA ACAACAGACA 
TTAGACACTG CTATGGACCC AAAAATGCAT CTATTGTTTG GCAGTGGGCA GGGGCTTATG 
GAATCGGAAT TCATTGACAG CGGTAGTACC AGTGCTTGTG GCAGCGGGTC ATCCAGTTTA 
GAACAAATGT CACAGTTAGT GCAAATGGAC AATTTAATTG ATTCGTCAAG TGGAGCCAAG 
CTTAAAGTTC CTCTTCAAAG TATCGGAGTA GATGTGCCAC CCCAAGTGCT GCAGGTCAGT 
ACAGTGCTTG AAAACCCTAC AAGGTATCAC GTTATCCAAA AGCAAAAGAA TCAAGTGAGG 
CAATATCTCA GCGAATCCTT TAAGCCGTCT ATGTGGGGCA GTCATACCAG CGAAATAAAA 
TTAGCAAATA ATTCTGCGTC AACGGGAAAT CTTCAAAATT CATCTTTACA GAAAGGTATT 
TGCGATCCAT TGGAGCGTAC AAACCGATTT GGATGCGATT CGGCTGTGAG TGCTAAAAGA 
ATTATGCCTT CCGATGACGC AATGCCTATT TCCCCATTCG GTGGTAGTTT TGTACGATGT 
GATGACATAA ATCCAATTGA GCCAACTGTA TTACGACCTA ATAGTCATGG TGCTGGGGAA 
CCAGAAAATG CACATAGAAC GGCCCAGTTG GGACTTAGTA AAGCTAATTC AAGCCTAAGT 
TCAACGCGTT CTTCTTCAGG GATTGTTAAT TCAATTCGAA TTTCCAGTAC ATCAAGCTCG 
TTACAGTCTA CCTCAGCTCC TATTTCGCCG AGCGTAAGTT CGGTTGCAAC GAGTGTCTCT 
GAGCCAGATG ACATCTTCGA TGATATATTA CAAAATGACT CTTTTAACTT TGATAAAAAC 
TTCAACTCTG AGCTTTCAAT TAAACAGGAA CCTCAAAATT TAACTGATGC TGAAATGAAT 
GCCCTAGCCA AGGACAGACA AAAGAAAGAC AACCATAATA TGATTGAGCG AAGGCGACGC 
TTTAATATAA ATGACAGAAT AAAAGAGCTT GGTACCCTTT TGCCAAAGGG CAGTGATGCT 
TTTTACGAAG TAGTTCGAGA CATTCGTCCA AACAAGGGGA CAATCTTGAA GTCCTCTGTT 
GACTACATTA AATGCTTGAA ACATGAGGTG ACTCGCTTAA GACAAAATGA GTTACGTCAA 
CGTCAAGTAG AGTTGCAAAA TCGAAAGCTC ATGTCTCGAA TAAAAGAATT GGAAATGCAG 
GCCAAGTCCC ATGGAATTTT GTTATCAGAA AACCACCTAA CATCACTATC AGCGCCTACT 
CAACCTTATC TTAAAAGTTT TAGCCTGTCT CCTACTGCTT CTCGGAGTCG TCGATCACTT 
TTTGACCAAC CTGTTGAAAA AAAAATACAA GTTATTGATG GTGCCGATGG AAATATGGGA 
ATGAATCAGG TTGATGAGTT TATGGAAGAT TGCAAGTATG CAGTACAAGG CGGCGATCCG 
ATGTTGTCCT CGCATAGTCA CATGCAATCC GCGCCACAAT CACCGAGTTC AAAAACTTTG 
AATAGTGGAA GTTTTGAACC AAAGAATTTT TCGGAGGCTG ATGAAAGCCT TTTTAGAGGC 
AAATCAAGTT TAGCATCAGA TGACTGTTGC GGTATAAATT GCAGTACTTC GTGCTATATT 
CAACACCAAT TGACTTCGCG TGAGGGCCAT CCAAATCATT CTCACCACAC GGGTATACGA 
GAAATTCATA GTTTGTCTGA TTCGGCTCAA AACTCAGAGT TTAGTAGGCT TGAGCATTGC 
GATGATCCAC TTCTCTCATC TTCCCATAGG TCACTTGGCA CCGTAGACGA AGATCAACAT 
AATTCAGTTG ATATGTCTGC AGTCATGGTA CACGACTCCC TATCCTCTTT GGTAGATGAC 
AACAATAGCG AAACAATGGT TCTTGCATCT GACACATTGG ATATTGAGTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mitf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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