Fife cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fife
Entrez Gene ID	38337
Full Name	CG43955 gene product from transcript CG43955-RD
Synonyms	CG12187,CG14950,CG16976,CG43375,CG43955,Dmel\CG43955,Dmel_CG12187,Dmel_CG14950,Dmel_CG16976,Dmel_CG43375,anon-EST:Posey171,fife
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62E8-62E8

mRNA and Protein(s)

mRNA	Protein	Name
NM_139485.2	NP_647742.1	fife, isoform G
NM_001274410.1	NP_001261339.1	fife, isoform F
NM_001274411.1	NP_001261340.1	fife, isoform D
NM_001274409.2	NP_001261338.1	fife, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003604	XP_313364.5
Drosophila melanogaster (fruit fly)	CG43955	NP_001261340.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fife organizes active zones to create synaptic vesicle release sites within nanometer distance of Ca(2+) channel clusters for reliable and modifiable neurotransmitter release.
Title: Fife organizes synaptic vesicles and calcium channels for high-probability neurotransmitter release.

The following Fife gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fife cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17320	NM_139485.2 Latest version!	Drosophila melanogaster fife, transcript variant G (Fife), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa17320	NM_001274410.1 Latest version!	Drosophila melanogaster fife, transcript variant F (Fife), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa20011	NM_001274409.2 Latest version!	Drosophila melanogaster fife, transcript variant B (Fife), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20012	NM_001274411.1 Latest version!	Drosophila melanogaster fife, transcript variant D (Fife), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fife ( NM_139485.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647742.1 Drosophila melanogaster fife, transcript variant G (Fife), mRNA.

Price & Availability↑

CloneID	OFa17320
Clone ID Related Accession (Same CDS sequence)	NM_139485.2 , NM_001274410.1
Accession Version	NM_139485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fife, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139485.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCAA CAAATGTGAT GTCATTCATG AAAAAGATGG TGGCCACCGA CGAGCAGACC 
AGCGAACGGC AGCAGCATGC AGCGGACTCG GGAGGAGGGG CCTCCCAAAC GGGGGCCCTG 
AACAAGATGA AGGCTACGAT TTCCTCCTCG CTGCTCACCG TCACAGATAA AGTTAATAAA 
ATGTCGCCAC GCCCCTCGCT GGTGCCGACG GATGCGGATA CCAGCGGCAG TTACGGCTCG 
CCCAGCTATC AAAGCCAGCA GCAGCAGCAG CAGGACTACG GCAGCAGTCT GAATAACAAC 
AATAACAACA GCAGCGGCGG CGGCACGGCG ACAACGGGCG GCGGTGGAGG TGGGGCGGCG 
GCCAAACAGG AGCCAGGACG AAGGGCCGGA GCCTGCCGGG TGTGTCTGAA GTCCTTCAAG 
CCGGACGACT ACCACAAGAC GTGCTTCGAG TGCCAGCAGC GAGTGTGCGA GGACTGCGCC 
AGCTACAGCA AGCTGGACGA GCACGAGGAT GCGACCATGT GGCGCTGCAG CGTGTGCCGC 
CGGAAAATGG CCTCGCGTGT GTGCATTCCG CAGGACTCGA CGGACTCGAT GCTGGACGTT 
CCGGTGCTGG AGGCACTGCA GCGGCGACAC TCGGACGCCA AGCTGGGCAG CAGCACGCAG 
ACCCTGGCCC CCAGCAATGG AGCAGCCCTT GCCCCGCCGC GAAGTCCCGA ACTGCGACGC 
CACTCGGATG TTTCGCCAGC CTCGCTGAAG GAGTTGGAGC GGCAACTGAA AGGCGGCCGC 
AGCATGGCGC CCAGTCGATC CAATAGTCCG CCGAGGGGCG AGGGCCCAGA TGGACAGCCC 
CCCTTCGGTG CGCCCCTGTC GCGCATGCAG TCGCGAAGGG GGTCGCGGGT GGCACGGCAG 
CACAGCTACG ATGACGACAT GAAGACCGGA CCCGTGGGTG GCAGCATGGG CGGTGGTGGT 
CCGGCGCTGG GTTTGGGTGC GGATGGAGCG GGCCTCGGCA TTCCGACCAT GCCACGCAGG 
AAGTCCGCCT ATGATGTGTT CGCCCCCGGG CTGACGCAAG GTGGTGCCAC TCCTGCCACG 
CAGCTGCAGA GATCGCCCGG CGAGGGTGGC ATCATGCCGC CCATCCAGCT GCCCGGATCC 
AGGCGACCCT CGTTCCGGGT GCCACATCCC TCGGAGGACA TCCAGAACGA CGACTCGCCC 
GGCTCGCCGG ATAAGGGCAG CCCCGTCCTG ACCGTCGACG ATGACCGGCG GATGCGGCGA 
CGCGGATCGC AGCTACCCGA TATAGCGATG CTGCAGAATC GTGGTGCCCT GCCTGCGGTT 
CCGCCCTCCT CGATTCCGCC GCCCATGGCT TCATTTACGG GCCCCAATCT GGAGGATTTG 
GAGGCGCCGC GACGGCAAAC ATCAATGGAC GGCGAGGCCA TACGGATCGT TATTCACGAC 
GTGGATTCGG GGCCGATTTG TGCATCTAAG CGGCGCATCA TTCTGCGCCG AGATCCCACG 
GACAAGGCGC ACCGCACACG TGGCTTTGGA ATGCGCGTCG TCGGCGGAAA AACAGGAGCC 
GATGGCCGTC TCTTCGCCTA CATTGTGTGG ACCGTTCCAG GAGGAGCGGC CGAGAAGAAT 
GGCCTGCAGC AGGGCGACAA GATACTCGAG TGGAATGGAA TGTCGCTGAT CGACCGAAGT 
TTCGAGGAGG TGTGCTCCAT CATGGACCGA ACCGGAGACG TTGTGGAGCT TCTGGTGGAG 
CATGCCACGG ACTTCCGGAT GTGCGATCTC TTCGATGAGA ACCTACCGCC CGGCAATGCC 
GGTGGCCAAA GCGGACCTCG GAGATCCGGA GACGGTCCCG TCGGACTGGG CCTCATAGCG 
GAACCCGAAA CCACCACAGA CAAATCACCA GCATCGCCAA CTAGACGGAA ATTACCAAAA 
ACTCCGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647742.1
CDS	979..2910
Translation	MLPTNVMSFMKKMVATDEQTSERQQHAADSGGGASQTGALNKMKATISSSLLTVTDKVNKMSPRPSLVPTDADTSGSYGSPSYQSQQQQQQDYGSSLNNNNNNSSGGGTATTGGGGGGAAAKQEPGRRAGACRVCLKSFKPDDYHKTCFECQQRVCEDCASYSKLDEHEDATMWRCSVCRRKMASRVCIPQDSTDSMLDVPVLEALQRRHSDAKLGSSTQTLAPSNGAALAPPRSPELRRHSDVSPASLKELERQLKGGRSMAPSRSNSPPRGEGPDGQPPFGAPLSRMQSRRGSRVARQHSYDDDMKTGPVGGSMGGGGPALGLGADGAGLGIPTMPRRKSAYDVFAPGLTQGGATPATQLQRSPGEGGIMPPIQLPGSRRPSFRVPHPSEDIQNDDSPGSPDKGSPVLTVDDDRRMRRRGSQLPDIAMLQNRGALPAVPPSSIPPPMASFTGPNLEDLEAPRRQTSMDGEAIRIVIHDVDSGPICASKRRIILRRDPTDKAHRTRGFGMRVVGGKTGADGRLFAYIVWTVPGGAAEKNGLQQGDKILEWNGMSLIDRSFEEVCSIMDRTGDVVELLVEHATDFRMCDLFDENLPPGNAGGQSGPRRSGDGPVGLGLIAEPETTTDKSPASPTRRKLPKTPV

Target ORF information:

RefSeq Version	NM_139485.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fife, transcript variant G (Fife), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139485.2

ORF Insert Sequence:

ATGTTGCCAA CAAATGTGAT GTCATTCATG AAAAAGATGG TGGCCACCGA CGAGCAGACC 
AGCGAACGGC AGCAGCATGC AGCGGACTCG GGAGGAGGGG CCTCCCAAAC GGGGGCCCTG 
AACAAGATGA AGGCTACGAT TTCCTCCTCG CTGCTCACCG TCACAGATAA AGTTAATAAA 
ATGTCGCCAC GCCCCTCGCT GGTGCCGACG GATGCGGATA CCAGCGGCAG TTACGGCTCG 
CCCAGCTATC AAAGCCAGCA GCAGCAGCAG CAGGACTACG GCAGCAGTCT GAATAACAAC 
AATAACAACA GCAGCGGCGG CGGCACGGCG ACAACGGGCG GCGGTGGAGG TGGGGCGGCG 
GCCAAACAGG AGCCAGGACG AAGGGCCGGA GCCTGCCGGG TGTGTCTGAA GTCCTTCAAG 
CCGGACGACT ACCACAAGAC GTGCTTCGAG TGCCAGCAGC GAGTGTGCGA GGACTGCGCC 
AGCTACAGCA AGCTGGACGA GCACGAGGAT GCGACCATGT GGCGCTGCAG CGTGTGCCGC 
CGGAAAATGG CCTCGCGTGT GTGCATTCCG CAGGACTCGA CGGACTCGAT GCTGGACGTT 
CCGGTGCTGG AGGCACTGCA GCGGCGACAC TCGGACGCCA AGCTGGGCAG CAGCACGCAG 
ACCCTGGCCC CCAGCAATGG AGCAGCCCTT GCCCCGCCGC GAAGTCCCGA ACTGCGACGC 
CACTCGGATG TTTCGCCAGC CTCGCTGAAG GAGTTGGAGC GGCAACTGAA AGGCGGCCGC 
AGCATGGCGC CCAGTCGATC CAATAGTCCG CCGAGGGGCG AGGGCCCAGA TGGACAGCCC 
CCCTTCGGTG CGCCCCTGTC GCGCATGCAG TCGCGAAGGG GGTCGCGGGT GGCACGGCAG 
CACAGCTACG ATGACGACAT GAAGACCGGA CCCGTGGGTG GCAGCATGGG CGGTGGTGGT 
CCGGCGCTGG GTTTGGGTGC GGATGGAGCG GGCCTCGGCA TTCCGACCAT GCCACGCAGG 
AAGTCCGCCT ATGATGTGTT CGCCCCCGGG CTGACGCAAG GTGGTGCCAC TCCTGCCACG 
CAGCTGCAGA GATCGCCCGG CGAGGGTGGC ATCATGCCGC CCATCCAGCT GCCCGGATCC 
AGGCGACCCT CGTTCCGGGT GCCACATCCC TCGGAGGACA TCCAGAACGA CGACTCGCCC 
GGCTCGCCGG ATAAGGGCAG CCCCGTCCTG ACCGTCGACG ATGACCGGCG GATGCGGCGA 
CGCGGATCGC AGCTACCCGA TATAGCGATG CTGCAGAATC GTGGTGCCCT GCCTGCGGTT 
CCGCCCTCCT CGATTCCGCC GCCCATGGCT TCATTTACGG GCCCCAATCT GGAGGATTTG 
GAGGCGCCGC GACGGCAAAC ATCAATGGAC GGCGAGGCCA TACGGATCGT TATTCACGAC 
GTGGATTCGG GGCCGATTTG TGCATCTAAG CGGCGCATCA TTCTGCGCCG AGATCCCACG 
GACAAGGCGC ACCGCACACG TGGCTTTGGA ATGCGCGTCG TCGGCGGAAA AACAGGAGCC 
GATGGCCGTC TCTTCGCCTA CATTGTGTGG ACCGTTCCAG GAGGAGCGGC CGAGAAGAAT 
GGCCTGCAGC AGGGCGACAA GATACTCGAG TGGAATGGAA TGTCGCTGAT CGACCGAAGT 
TTCGAGGAGG TGTGCTCCAT CATGGACCGA ACCGGAGACG TTGTGGAGCT TCTGGTGGAG 
CATGCCACGG ACTTCCGGAT GTGCGATCTC TTCGATGAGA ACCTACCGCC CGGCAATGCC 
GGTGGCCAAA GCGGACCTCG GAGATCCGGA GACGGTCCCG TCGGACTGGG CCTCATAGCG 
GAACCCGAAA CCACCACAGA CAAATCACCA GCATCGCCAA CTAGACGGAA ATTACCAAAA 
ACTCCGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fife ( NM_001274410.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261339.1 Drosophila melanogaster fife, transcript variant F (Fife), mRNA.

Price & Availability↑

CloneID	OFa17320
Clone ID Related Accession (Same CDS sequence)	NM_139485.2 , NM_001274410.1
Accession Version	NM_001274410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fife, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCAA CAAATGTGAT GTCATTCATG AAAAAGATGG TGGCCACCGA CGAGCAGACC 
AGCGAACGGC AGCAGCATGC AGCGGACTCG GGAGGAGGGG CCTCCCAAAC GGGGGCCCTG 
AACAAGATGA AGGCTACGAT TTCCTCCTCG CTGCTCACCG TCACAGATAA AGTTAATAAA 
ATGTCGCCAC GCCCCTCGCT GGTGCCGACG GATGCGGATA CCAGCGGCAG TTACGGCTCG 
CCCAGCTATC AAAGCCAGCA GCAGCAGCAG CAGGACTACG GCAGCAGTCT GAATAACAAC 
AATAACAACA GCAGCGGCGG CGGCACGGCG ACAACGGGCG GCGGTGGAGG TGGGGCGGCG 
GCCAAACAGG AGCCAGGACG AAGGGCCGGA GCCTGCCGGG TGTGTCTGAA GTCCTTCAAG 
CCGGACGACT ACCACAAGAC GTGCTTCGAG TGCCAGCAGC GAGTGTGCGA GGACTGCGCC 
AGCTACAGCA AGCTGGACGA GCACGAGGAT GCGACCATGT GGCGCTGCAG CGTGTGCCGC 
CGGAAAATGG CCTCGCGTGT GTGCATTCCG CAGGACTCGA CGGACTCGAT GCTGGACGTT 
CCGGTGCTGG AGGCACTGCA GCGGCGACAC TCGGACGCCA AGCTGGGCAG CAGCACGCAG 
ACCCTGGCCC CCAGCAATGG AGCAGCCCTT GCCCCGCCGC GAAGTCCCGA ACTGCGACGC 
CACTCGGATG TTTCGCCAGC CTCGCTGAAG GAGTTGGAGC GGCAACTGAA AGGCGGCCGC 
AGCATGGCGC CCAGTCGATC CAATAGTCCG CCGAGGGGCG AGGGCCCAGA TGGACAGCCC 
CCCTTCGGTG CGCCCCTGTC GCGCATGCAG TCGCGAAGGG GGTCGCGGGT GGCACGGCAG 
CACAGCTACG ATGACGACAT GAAGACCGGA CCCGTGGGTG GCAGCATGGG CGGTGGTGGT 
CCGGCGCTGG GTTTGGGTGC GGATGGAGCG GGCCTCGGCA TTCCGACCAT GCCACGCAGG 
AAGTCCGCCT ATGATGTGTT CGCCCCCGGG CTGACGCAAG GTGGTGCCAC TCCTGCCACG 
CAGCTGCAGA GATCGCCCGG CGAGGGTGGC ATCATGCCGC CCATCCAGCT GCCCGGATCC 
AGGCGACCCT CGTTCCGGGT GCCACATCCC TCGGAGGACA TCCAGAACGA CGACTCGCCC 
GGCTCGCCGG ATAAGGGCAG CCCCGTCCTG ACCGTCGACG ATGACCGGCG GATGCGGCGA 
CGCGGATCGC AGCTACCCGA TATAGCGATG CTGCAGAATC GTGGTGCCCT GCCTGCGGTT 
CCGCCCTCCT CGATTCCGCC GCCCATGGCT TCATTTACGG GCCCCAATCT GGAGGATTTG 
GAGGCGCCGC GACGGCAAAC ATCAATGGAC GGCGAGGCCA TACGGATCGT TATTCACGAC 
GTGGATTCGG GGCCGATTTG TGCATCTAAG CGGCGCATCA TTCTGCGCCG AGATCCCACG 
GACAAGGCGC ACCGCACACG TGGCTTTGGA ATGCGCGTCG TCGGCGGAAA AACAGGAGCC 
GATGGCCGTC TCTTCGCCTA CATTGTGTGG ACCGTTCCAG GAGGAGCGGC CGAGAAGAAT 
GGCCTGCAGC AGGGCGACAA GATACTCGAG TGGAATGGAA TGTCGCTGAT CGACCGAAGT 
TTCGAGGAGG TGTGCTCCAT CATGGACCGA ACCGGAGACG TTGTGGAGCT TCTGGTGGAG 
CATGCCACGG ACTTCCGGAT GTGCGATCTC TTCGATGAGA ACCTACCGCC CGGCAATGCC 
GGTGGCCAAA GCGGACCTCG GAGATCCGGA GACGGTCCCG TCGGACTGGG CCTCATAGCG 
GAACCCGAAA CCACCACAGA CAAATCACCA GCATCGCCAA CTAGACGGAA ATTACCAAAA 
ACTCCGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261339.1
CDS	985..2916
Translation	MLPTNVMSFMKKMVATDEQTSERQQHAADSGGGASQTGALNKMKATISSSLLTVTDKVNKMSPRPSLVPTDADTSGSYGSPSYQSQQQQQQDYGSSLNNNNNNSSGGGTATTGGGGGGAAAKQEPGRRAGACRVCLKSFKPDDYHKTCFECQQRVCEDCASYSKLDEHEDATMWRCSVCRRKMASRVCIPQDSTDSMLDVPVLEALQRRHSDAKLGSSTQTLAPSNGAALAPPRSPELRRHSDVSPASLKELERQLKGGRSMAPSRSNSPPRGEGPDGQPPFGAPLSRMQSRRGSRVARQHSYDDDMKTGPVGGSMGGGGPALGLGADGAGLGIPTMPRRKSAYDVFAPGLTQGGATPATQLQRSPGEGGIMPPIQLPGSRRPSFRVPHPSEDIQNDDSPGSPDKGSPVLTVDDDRRMRRRGSQLPDIAMLQNRGALPAVPPSSIPPPMASFTGPNLEDLEAPRRQTSMDGEAIRIVIHDVDSGPICASKRRIILRRDPTDKAHRTRGFGMRVVGGKTGADGRLFAYIVWTVPGGAAEKNGLQQGDKILEWNGMSLIDRSFEEVCSIMDRTGDVVELLVEHATDFRMCDLFDENLPPGNAGGQSGPRRSGDGPVGLGLIAEPETTTDKSPASPTRRKLPKTPV

Target ORF information:

RefSeq Version	NM_001274410.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fife, transcript variant F (Fife), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274410.1

ORF Insert Sequence:

ATGTTGCCAA CAAATGTGAT GTCATTCATG AAAAAGATGG TGGCCACCGA CGAGCAGACC 
AGCGAACGGC AGCAGCATGC AGCGGACTCG GGAGGAGGGG CCTCCCAAAC GGGGGCCCTG 
AACAAGATGA AGGCTACGAT TTCCTCCTCG CTGCTCACCG TCACAGATAA AGTTAATAAA 
ATGTCGCCAC GCCCCTCGCT GGTGCCGACG GATGCGGATA CCAGCGGCAG TTACGGCTCG 
CCCAGCTATC AAAGCCAGCA GCAGCAGCAG CAGGACTACG GCAGCAGTCT GAATAACAAC 
AATAACAACA GCAGCGGCGG CGGCACGGCG ACAACGGGCG GCGGTGGAGG TGGGGCGGCG 
GCCAAACAGG AGCCAGGACG AAGGGCCGGA GCCTGCCGGG TGTGTCTGAA GTCCTTCAAG 
CCGGACGACT ACCACAAGAC GTGCTTCGAG TGCCAGCAGC GAGTGTGCGA GGACTGCGCC 
AGCTACAGCA AGCTGGACGA GCACGAGGAT GCGACCATGT GGCGCTGCAG CGTGTGCCGC 
CGGAAAATGG CCTCGCGTGT GTGCATTCCG CAGGACTCGA CGGACTCGAT GCTGGACGTT 
CCGGTGCTGG AGGCACTGCA GCGGCGACAC TCGGACGCCA AGCTGGGCAG CAGCACGCAG 
ACCCTGGCCC CCAGCAATGG AGCAGCCCTT GCCCCGCCGC GAAGTCCCGA ACTGCGACGC 
CACTCGGATG TTTCGCCAGC CTCGCTGAAG GAGTTGGAGC GGCAACTGAA AGGCGGCCGC 
AGCATGGCGC CCAGTCGATC CAATAGTCCG CCGAGGGGCG AGGGCCCAGA TGGACAGCCC 
CCCTTCGGTG CGCCCCTGTC GCGCATGCAG TCGCGAAGGG GGTCGCGGGT GGCACGGCAG 
CACAGCTACG ATGACGACAT GAAGACCGGA CCCGTGGGTG GCAGCATGGG CGGTGGTGGT 
CCGGCGCTGG GTTTGGGTGC GGATGGAGCG GGCCTCGGCA TTCCGACCAT GCCACGCAGG 
AAGTCCGCCT ATGATGTGTT CGCCCCCGGG CTGACGCAAG GTGGTGCCAC TCCTGCCACG 
CAGCTGCAGA GATCGCCCGG CGAGGGTGGC ATCATGCCGC CCATCCAGCT GCCCGGATCC 
AGGCGACCCT CGTTCCGGGT GCCACATCCC TCGGAGGACA TCCAGAACGA CGACTCGCCC 
GGCTCGCCGG ATAAGGGCAG CCCCGTCCTG ACCGTCGACG ATGACCGGCG GATGCGGCGA 
CGCGGATCGC AGCTACCCGA TATAGCGATG CTGCAGAATC GTGGTGCCCT GCCTGCGGTT 
CCGCCCTCCT CGATTCCGCC GCCCATGGCT TCATTTACGG GCCCCAATCT GGAGGATTTG 
GAGGCGCCGC GACGGCAAAC ATCAATGGAC GGCGAGGCCA TACGGATCGT TATTCACGAC 
GTGGATTCGG GGCCGATTTG TGCATCTAAG CGGCGCATCA TTCTGCGCCG AGATCCCACG 
GACAAGGCGC ACCGCACACG TGGCTTTGGA ATGCGCGTCG TCGGCGGAAA AACAGGAGCC 
GATGGCCGTC TCTTCGCCTA CATTGTGTGG ACCGTTCCAG GAGGAGCGGC CGAGAAGAAT 
GGCCTGCAGC AGGGCGACAA GATACTCGAG TGGAATGGAA TGTCGCTGAT CGACCGAAGT 
TTCGAGGAGG TGTGCTCCAT CATGGACCGA ACCGGAGACG TTGTGGAGCT TCTGGTGGAG 
CATGCCACGG ACTTCCGGAT GTGCGATCTC TTCGATGAGA ACCTACCGCC CGGCAATGCC 
GGTGGCCAAA GCGGACCTCG GAGATCCGGA GACGGTCCCG TCGGACTGGG CCTCATAGCG 
GAACCCGAAA CCACCACAGA CAAATCACCA GCATCGCCAA CTAGACGGAA ATTACCAAAA 
ACTCCGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fife ( NM_001274409.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261338.1 Drosophila melanogaster fife, transcript variant B (Fife), mRNA.

Price & Availability↑

CloneID	OFa20011
Clone ID Related Accession (Same CDS sequence)	NM_001274409.2
Accession Version	NM_001274409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fife, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001274409.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCAA CAAATGTGAT GTCATTCATG AAAAAGATGG TGGCCACCGA CGAGCAGACC 
AGCGAACGGC AGCAGCATGC AGCGGACTCG GGAGGAGGGG CCTCCCAAAC GGGGGCCCTG 
AACAAGATGA AGGCTACGAT TTCCTCCTCG CTGCTCACCG TCACAGATAA AGTTAATAAA 
ATGTCGCCAC GCCCCTCGCT GGTGCCGACG GATGCGGATA CCAGCGGCAG TTACGGCTCG 
CCCAGCTATC AAAGCCAGCA GCAGCAGCAG CAGGACTACG GCAGCAGTCT GAATAACAAC 
AATAACAACA GCAGCGGCGG CGGCACGGCG ACAACGGGCG GCGGTGGAGG TGGGGCGGCG 
GCCAAACAGG AGCCAGGACG AAGGGCCGGA GCCTGCCGGG TGTGTCTGAA GTCCTTCAAG 
CCGGACGACT ACCACAAGAC GTGCTTCGAG TGCCAGCAGC GAGTGTGCGA GGACTGCGCC 
AGCTACAGCA AGCTGGACGA GCACGAGGAT GCGACCATGT GGCGCTGCAG CGTGTGCCGC 
CGGAAAATGG CCTCGCGTGT GTGCATTCCG CAGGACTCGA CGGACTCGAT GCTGGACGTT 
CCGGTGCTGG AGGCACTGCA GCGGCGACAC TCGGACGCCA AGCTGGGCAG CAGCACGCAG 
ACCCTGGCCC CCAGCAATGG AGCAGCCCTT GCCCCGCCGC GAAGTCCCGA ACTGCGACGC 
CACTCGGATG TTTCGCCAGC CTCGCTGAAG GAGTTGGAGC GGCAACTGAA AGGCGGCCGC 
AGCATGGCGC CCAGTCGATC CAATAGTCCG CCGAGGGGCG AGGGCCCAGA TGGACAGCCC 
CCCTTCGGTG CGCCCCTGTC GCGCATGCAG TCGCGAAGGG GGTCGCGGGT GGCACGGCAG 
CACAGCTACG ATGACGACAT GAAGACCGGA CCCGTGGGTG GCAGCATGGG CGGTGGTGGT 
CCGGCGCTGG GTTTGGGTGC GGATGGAGCG GGCCTCGGCA TTCCGACCAT GCCACGCAGG 
AAGTCCGCCT ATGATGTGTT CGCCCCCGGG CTGACGCAAG GTGGTGCCAC TCCTGCCACG 
CAGCTGCAGA GATCGCCCGG CGAGGGTGGC ATCATGCCGC CCATCCAGCT GCCCGGATCC 
AGGCGACCCT CGTTCCGGGT GCCACATCCC TCGGAGGACA TCCAGAACGA CGACTCGCCC 
GGCTCGCCGG ATAAGGGCAG CCCCGTCCTG ACCGTCGACG ATGACCGGCG GATGCGGCGA 
CGCGGATCGC AGCTACCCGA TATAGCGATG CTGCAGAATC GTGGTGCCCT GCCTGCGGTT 
CCGCCCTCCT CGATTCCGCC GCCCATGGCT TCATTTACGG GCCCCAATCT GGAGGATTTG 
GAGGCGCCGC GACGGCAAAC ATCAATGGAC GGCGAGGCCA TACGGATCGT TATTCACGAC 
GTGGATTCGG GGCCGATTTG TGCATCTAAG CGGCGCATCA TTCTGCGCCG AGATCCCACG 
GACAAGGCGC ACCGCACACG TGGCTTTGGA ATGCGCGTCG TCGGCGGAAA AACAGGAGCC 
GATGGCCGTC TCTTCGCCTA CATTGTGTGG ACCGTTCCAG GAGGAGCGGC CGAGAAGAAT 
GGCCTGCAGC AGGGCGACAA GATACTCGAG TGGAATGGAA TGTCGCTGAT CGACCGAAGT 
TTCGAGGAGG TGTGCTCCAT CATGGACCGA ACCGGAGACG TTGTGGAGCT TCTGGTGGAG 
CATGCCACGG ACTTCCGGAT GTGCGATCTC TTCGATGAGA ACCTACCGCC CGGCAATGCC 
GGTGGCCAAA GCGGACCTCG GAGATCCGGA GACGGTCCCG TCGGACTGGG CCTCATAGCG 
GAACCCGAAA CCACCACAGA CAAATCACCA GCATCGCCAA CTAGACGGAA ATTACCAAAA 
ACTCCGGAGC AAATTGCCCG CGAGAAGCTG GTCACCGGAA GGGTTCAGAT CCAGGTTTGG 
TACCATGCGG AGCGCAGCGA GCTGGTCGTC TCCCTGATGG CCGGCGACGA CCTGGCACCG 
CGGGATGAGG CCTACGGACA TGGCAATCTG CCCGAGGCGT ACGCCAAGGT CCGCATTCTG 
CCGAAATGTG GAGATGGCAG CGTGCAACAA ACGGAGGTCA GCCGACCCAC ACAGAATCCC 
ATTTGGAACG CTACACTGAC CTTCGGCCAT GTGAAGGCCG ACACCTTGAT GGTTCGCTAC 
ATAGACATTC AGCTGTGGGA CCTGGTACCC CACACCGAGT CCATTTTCCT GGGCGAGTGC 
AGCATCGAGC TGCAGCAGGC CTTTCTCGAT GACCAGGCAA TATGGTGCCG CTTGGAGGAT 
ACGAAGGGGC TGCGTGGCAT AAGCATTAGC AAATCACCGA GTGTATCGCC ACGTGGCTCG 
ATTGCAGCCG GCGCAGGGGC GCCCAGTGGC GATGTGACCC GGTTGCTGCG TCGCGACTAC 
AATATGCAGC GCTCCAACTC CGATGACGTA GACTCCATCG GAGATGGAAC TTCACTGCTG 
CATCCGGATC ACGCCTGGAT AGCTGGCTCG CGACGCGGTT CCTCTCAGTC GGAAACCATG 
GAGGTGGAGG TCTACCAGCT GGGCAAGGAC TTCTCGCGCT CATTGCCGGG TTCGCGTCGA 
TCGAGCTTCC AGGATGCCGA AAAGAATCGG CTGGAGGAGG ACGCCATGGC CACGCCGCCC 
ACCTCTTATC TGGTGGGCCG GCGCCGTTCG TCCGTGGCTC GGCGCGATCC GGATGAGATC 
TTGAAATCCT TGAAGGCTGT CCGCGGTGAG CTGGGGCGCA CCATGAGTCT GGGTACCGAG 
CAGCACAAGC GCATGGGATC GAGACCCACA ATATCGGTGT CGCATTATCA AAGCTTTGAC 
ACACCCTACA CAAACTGGAC AAAACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261338.1
CDS	985..3894
Translation	MLPTNVMSFMKKMVATDEQTSERQQHAADSGGGASQTGALNKMKATISSSLLTVTDKVNKMSPRPSLVPTDADTSGSYGSPSYQSQQQQQQDYGSSLNNNNNNSSGGGTATTGGGGGGAAAKQEPGRRAGACRVCLKSFKPDDYHKTCFECQQRVCEDCASYSKLDEHEDATMWRCSVCRRKMASRVCIPQDSTDSMLDVPVLEALQRRHSDAKLGSSTQTLAPSNGAALAPPRSPELRRHSDVSPASLKELERQLKGGRSMAPSRSNSPPRGEGPDGQPPFGAPLSRMQSRRGSRVARQHSYDDDMKTGPVGGSMGGGGPALGLGADGAGLGIPTMPRRKSAYDVFAPGLTQGGATPATQLQRSPGEGGIMPPIQLPGSRRPSFRVPHPSEDIQNDDSPGSPDKGSPVLTVDDDRRMRRRGSQLPDIAMLQNRGALPAVPPSSIPPPMASFTGPNLEDLEAPRRQTSMDGEAIRIVIHDVDSGPICASKRRIILRRDPTDKAHRTRGFGMRVVGGKTGADGRLFAYIVWTVPGGAAEKNGLQQGDKILEWNGMSLIDRSFEEVCSIMDRTGDVVELLVEHATDFRMCDLFDENLPPGNAGGQSGPRRSGDGPVGLGLIAEPETTTDKSPASPTRRKLPKTPEQIAREKLVTGRVQIQVWYHAERSELVVSLMAGDDLAPRDEAYGHGNLPEAYAKVRILPKCGDGSVQQTEVSRPTQNPIWNATLTFGHVKADTLMVRYIDIQLWDLVPHTESIFLGECSIELQQAFLDDQAIWCRLEDTKGLRGISISKSPSVSPRGSIAAGAGAPSGDVTRLLRRDYNMQRSNSDDVDSIGDGTSLLHPDHAWIAGSRRGSSQSETMEVEVYQLGKDFSRSLPGSRRSSFQDAEKNRLEEDAMATPPTSYLVGRRRSSVARRDPDEILKSLKAVRGELGRTMSLGTEQHKRMGSRPTISVSHYQSFDTPYTNWTKH

Target ORF information:

RefSeq Version	NM_001274409.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fife, transcript variant B (Fife), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274409.2

ORF Insert Sequence:

ATGTTGCCAA CAAATGTGAT GTCATTCATG AAAAAGATGG TGGCCACCGA CGAGCAGACC 
AGCGAACGGC AGCAGCATGC AGCGGACTCG GGAGGAGGGG CCTCCCAAAC GGGGGCCCTG 
AACAAGATGA AGGCTACGAT TTCCTCCTCG CTGCTCACCG TCACAGATAA AGTTAATAAA 
ATGTCGCCAC GCCCCTCGCT GGTGCCGACG GATGCGGATA CCAGCGGCAG TTACGGCTCG 
CCCAGCTATC AAAGCCAGCA GCAGCAGCAG CAGGACTACG GCAGCAGTCT GAATAACAAC 
AATAACAACA GCAGCGGCGG CGGCACGGCG ACAACGGGCG GCGGTGGAGG TGGGGCGGCG 
GCCAAACAGG AGCCAGGACG AAGGGCCGGA GCCTGCCGGG TGTGTCTGAA GTCCTTCAAG 
CCGGACGACT ACCACAAGAC GTGCTTCGAG TGCCAGCAGC GAGTGTGCGA GGACTGCGCC 
AGCTACAGCA AGCTGGACGA GCACGAGGAT GCGACCATGT GGCGCTGCAG CGTGTGCCGC 
CGGAAAATGG CCTCGCGTGT GTGCATTCCG CAGGACTCGA CGGACTCGAT GCTGGACGTT 
CCGGTGCTGG AGGCACTGCA GCGGCGACAC TCGGACGCCA AGCTGGGCAG CAGCACGCAG 
ACCCTGGCCC CCAGCAATGG AGCAGCCCTT GCCCCGCCGC GAAGTCCCGA ACTGCGACGC 
CACTCGGATG TTTCGCCAGC CTCGCTGAAG GAGTTGGAGC GGCAACTGAA AGGCGGCCGC 
AGCATGGCGC CCAGTCGATC CAATAGTCCG CCGAGGGGCG AGGGCCCAGA TGGACAGCCC 
CCCTTCGGTG CGCCCCTGTC GCGCATGCAG TCGCGAAGGG GGTCGCGGGT GGCACGGCAG 
CACAGCTACG ATGACGACAT GAAGACCGGA CCCGTGGGTG GCAGCATGGG CGGTGGTGGT 
CCGGCGCTGG GTTTGGGTGC GGATGGAGCG GGCCTCGGCA TTCCGACCAT GCCACGCAGG 
AAGTCCGCCT ATGATGTGTT CGCCCCCGGG CTGACGCAAG GTGGTGCCAC TCCTGCCACG 
CAGCTGCAGA GATCGCCCGG CGAGGGTGGC ATCATGCCGC CCATCCAGCT GCCCGGATCC 
AGGCGACCCT CGTTCCGGGT GCCACATCCC TCGGAGGACA TCCAGAACGA CGACTCGCCC 
GGCTCGCCGG ATAAGGGCAG CCCCGTCCTG ACCGTCGACG ATGACCGGCG GATGCGGCGA 
CGCGGATCGC AGCTACCCGA TATAGCGATG CTGCAGAATC GTGGTGCCCT GCCTGCGGTT 
CCGCCCTCCT CGATTCCGCC GCCCATGGCT TCATTTACGG GCCCCAATCT GGAGGATTTG 
GAGGCGCCGC GACGGCAAAC ATCAATGGAC GGCGAGGCCA TACGGATCGT TATTCACGAC 
GTGGATTCGG GGCCGATTTG TGCATCTAAG CGGCGCATCA TTCTGCGCCG AGATCCCACG 
GACAAGGCGC ACCGCACACG TGGCTTTGGA ATGCGCGTCG TCGGCGGAAA AACAGGAGCC 
GATGGCCGTC TCTTCGCCTA CATTGTGTGG ACCGTTCCAG GAGGAGCGGC CGAGAAGAAT 
GGCCTGCAGC AGGGCGACAA GATACTCGAG TGGAATGGAA TGTCGCTGAT CGACCGAAGT 
TTCGAGGAGG TGTGCTCCAT CATGGACCGA ACCGGAGACG TTGTGGAGCT TCTGGTGGAG 
CATGCCACGG ACTTCCGGAT GTGCGATCTC TTCGATGAGA ACCTACCGCC CGGCAATGCC 
GGTGGCCAAA GCGGACCTCG GAGATCCGGA GACGGTCCCG TCGGACTGGG CCTCATAGCG 
GAACCCGAAA CCACCACAGA CAAATCACCA GCATCGCCAA CTAGACGGAA ATTACCAAAA 
ACTCCGGAGC AAATTGCCCG CGAGAAGCTG GTCACCGGAA GGGTTCAGAT CCAGGTTTGG 
TACCATGCGG AGCGCAGCGA GCTGGTCGTC TCCCTGATGG CCGGCGACGA CCTGGCACCG 
CGGGATGAGG CCTACGGACA TGGCAATCTG CCCGAGGCGT ACGCCAAGGT CCGCATTCTG 
CCGAAATGTG GAGATGGCAG CGTGCAACAA ACGGAGGTCA GCCGACCCAC ACAGAATCCC 
ATTTGGAACG CTACACTGAC CTTCGGCCAT GTGAAGGCCG ACACCTTGAT GGTTCGCTAC 
ATAGACATTC AGCTGTGGGA CCTGGTACCC CACACCGAGT CCATTTTCCT GGGCGAGTGC 
AGCATCGAGC TGCAGCAGGC CTTTCTCGAT GACCAGGCAA TATGGTGCCG CTTGGAGGAT 
ACGAAGGGGC TGCGTGGCAT AAGCATTAGC AAATCACCGA GTGTATCGCC ACGTGGCTCG 
ATTGCAGCCG GCGCAGGGGC GCCCAGTGGC GATGTGACCC GGTTGCTGCG TCGCGACTAC 
AATATGCAGC GCTCCAACTC CGATGACGTA GACTCCATCG GAGATGGAAC TTCACTGCTG 
CATCCGGATC ACGCCTGGAT AGCTGGCTCG CGACGCGGTT CCTCTCAGTC GGAAACCATG 
GAGGTGGAGG TCTACCAGCT GGGCAAGGAC TTCTCGCGCT CATTGCCGGG TTCGCGTCGA 
TCGAGCTTCC AGGATGCCGA AAAGAATCGG CTGGAGGAGG ACGCCATGGC CACGCCGCCC 
ACCTCTTATC TGGTGGGCCG GCGCCGTTCG TCCGTGGCTC GGCGCGATCC GGATGAGATC 
TTGAAATCCT TGAAGGCTGT CCGCGGTGAG CTGGGGCGCA CCATGAGTCT GGGTACCGAG 
CAGCACAAGC GCATGGGATC GAGACCCACA ATATCGGTGT CGCATTATCA AAGCTTTGAC 
ACACCCTACA CAAACTGGAC AAAACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fife ( NM_001274411.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261340.1 Drosophila melanogaster fife, transcript variant D (Fife), mRNA.

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CloneID	OFa20012
Clone ID Related Accession (Same CDS sequence)	NM_001274411.1
Accession Version	NM_001274411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fife, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCAA CAAATGTGAT GTCATTCATG AAAAAGATGG TGGCCACCGA CGAGCAGACC 
AGCGAACGGC AGCAGCATGC AGCGGACTCG GGAGGAGGGG CCTCCCAAAC GGGGGCCCTG 
AACAAGATGA AGGCTACGAT TTCCTCCTCG CTGCTCACCG TCACAGATAA AGTTAATAAA 
ATGTCGCCAC GCCCCTCGCT GGTGCCGACG GATGCGGATA CCAGCGGCAG TTACGGCTCG 
CCCAGCTATC AAAGCCAGCA GCAGCAGCAG CAGGACTACG GCAGCAGTCT GAATAACAAC 
AATAACAACA GCAGCGGCGG CGGCACGGCG ACAACGGGCG GCGGTGGAGG TGGGGCGGCG 
GCCAAACAGG AGCCAGGACG AAGGGCCGGA GCCTGCCGGG TGTGTCTGAA GTCCTTCAAG 
CCGGACGACT ACCACAAGAC GTGCTTCGAG TGCCAGCAGC GAGTGTGCGA GGACTGCGCC 
AGCTACAGCA AGCTGGACGA GCACGAGGAT GCGACCATGT GGCGCTGCAG CGTGTGCCGC 
CGGAAAATGG CCTCGCGTGT GTGCATTCCG CAGGACTCGA CGGACTCGAT GCTGGACGTT 
CCGGTGCTGG AGGCACTGCA GCGGCGACAC TCGGACGCCA AGCTGGGCAG CAGCACGCAG 
ACCCTGGCCC CCAGCAATGG AGCAGCCCTT GCCCCGCCGC GAAGTCCCGA ACTGCGACGC 
CACTCGGATG TTTCGCCAGC CTCGCTGAAG GAGTTGGAGC GGCAACTGAA AGGCGGCCGC 
AGCATGGCGC CCAGTCGATC CAATAGTCCG CCGAGGGGCG AGGGCCCAGA TGGACAGCCC 
CCCTTCGGTG CGCCCCTGTC GCGCATGCAG TCGCGAAGGG GGTCGCGGGT GGCACGGCAG 
CACAGCTACG ATGACGACAT GAAGACCGGA CCCGTGGGTG GCAGCATGGG CGGTGGTGGT 
CCGGCGCTGG GTTTGGGTGC GGATGGAGCG GGCCTCGGCA TTCCGACCAT GCCACGCAGG 
AAGTCCGCCT ATGATGTGTT CGCCCCCGGG CTGACGCAAG GTGGTGCCAC TCCTGCCACG 
CAGCTGCAGA GATCGCCCGG CGAGGGTGGC ATCATGCCGC CCATCCAGCT GCCCGGATCC 
AGGCGACCCT CGTTCCGGGT GCCACATCCC TCGGAGGACA TCCAGAACGA CGACTCGCCC 
GGCTCGCCGG ATAAGGGCAG CCCCGTCCTG ACCGTCGACG ATGACCGGCG GATGCGGCGA 
CGCGGATCGC AGCTACCCGA TATAGCGATG CTGCAGAATC GTGGTGCCCT GCCTGCGGTT 
CCGCCCTCCT CGATTCCGCC GCCCATGGCT TCATTTACGG GCCCCAATCT GGAGGATTTG 
GAGGCGCCGC GACGGCAAAC ATCAATGGAC GGCGAGGCCA TACGGATCGT TATTCACGAC 
GTGGATTCGG GGCCGATTTG TGCATCTAAG CGGCGCATCA TTCTGCGCCG AGATCCCACG 
GACAAGGCGC ACCGCACACG TGGCTTTGGA ATGCGCGTCG TCGGCGGAAA AACAGGAGCC 
GATGGCCGTC TCTTCGCCTA CATTGTGTGG ACCGTTCCAG GAGGAGCGGC CGAGAAGAAT 
GGCCTGCAGC AGGGCGACAA GATACTCGAG TGGAATGGAA TGTCGCTGAT CGACCGAAGT 
TTCGAGGAGG TGTGCTCCAT CATGGACCGA ACCGGAGACG TTGTGGAGCT TCTGGTGGAG 
CATGCCACGG ACTTCCGGAT GTGCGATCTC TTCGATGAGA ACCTACCGCC CGGCAATGCC 
GGTGGCCAAA GCGGACCTCG GAGATCCGGA GACGGTCCCG TCGGACTGGG CCTCATAGCG 
GAACCCGAAA CCACCACAGA CAAATCACCA GCATCGCCAA CTAGACGGAA ATTACCAAAA 
ACTCCGGAGC AAATTGCCCG CGAGAAGCTG GTCACCGGAA GGGTTCAGAT CCAGGTTTGG 
TACCATGCGG AGCGCAGCGA GCTGGTCGTC TCCCTGATGG CCGGCGACGA CCTGGCACCG 
CGGGATGAGG CCTACGGACA TGGCAATCTG CCCGAGGCGT ACGCCAAGGT CCGCATTCTG 
CCGAAATGTG GAGATGGCAG CGTGCAACAA ACGGAGGTCA GCCGACCCAC ACAGAATCCC 
ATTTGGAACG CTACACTGAC CTTCGGCCAT GTGAAGGCCG ACACCTTGAT GGTTCGCTAC 
ATAGACATTC AGCTGTGGGA CCTGGTACCC CACACCGAGT CCATTTTCCT GGGCGAGTGC 
AGCATCGAGC TGCAGCAGGC CTTTCTCGAT GACCAGGCAA TATGGTGCCG CTTGGAGGAT 
ACGAAGGGGC TGCGTGGCAT AAGCATTAGC AAATCACCGA GTGTATCGCC ACGTGGCTCG 
ATTGCAGCCG GCGCAGGGGC GCCCAGTGGC GATGTGACCC GGTTGCTGCG TCGCGACTAC 
AATATGCAGC GCTCCAACTC CGATGACGTA GACTCCATCG GAGATGGAAC TTCACTGCTG 
CATCCGGATC ACGCCTGGAT AGCTGGCTCG CGACGCGGTT CCTCTCAGTC GGAAACCATG 
GAGGTGGAGG TCTACCAGCT GGGCAAGGAC TTCTCGCGCT CATTGCCGGG TTCGCGTCGA 
TCGAGCTTCC AGGATGCCGA AAAGAATCGG CTGGAGGAGG ACGCCATGGC CACGCCGCCC 
ACCTCTTATC TGGTGGGCCG GCGCCGTTCG TCCGTGGCTC GGCGCGATCC GGATGAGATC 
TTGAAATCCT TGAAGGCTGT CCGCGGTGAG CTGGGGCGCA CCATGAGTCT GGGTACCGAG 
CAGCACAAGC GCATGGGATC GAGACGTGCC AGTCGCGTTG GATTGCCGAG CGGACCGAGT 
AGCCGGAAGA GTTCCGTCTT AGATTTGTCG CAGCAGCATC AGCAGCAGTT GCAGCAGCTG 
CAGCAGTTGC AGTTGCAGCA GCAGCAGTAC CTGCAGCAGC AGCTGCCCAT GGGAGTGGGC 
AATGCCGACA CCAGTCCACC GTCCGAAGAC GAGGATAAGC GCTACAGGCC GCGCTGGAAG 
GGATCGGTCA GCCAGTTGCC GCCGCAGAGT CCCAAGCAGG CGCTATCCCG GCTCAAGCAG 
GCCGCATCCG CGTCGAACGT GGCCCAGGAC ACGCAGCGAC AGGGCCAACT GCTGACCACC 
AACCCACTGC AGCAACGCAA GAGCAGCATG TTCCAACTGG GCGAGTCCCA GGCATCGCCC 
CCAACGACCA CCACCACCCA CTTGCAGCGG AAGGGCAGCA TGTACGTCGC CAAGGTCACC 
GAGACACCGC CCCACGGTAC GCCCACCCGA AAGGGCAGCG TCTACCAGAG AAGCGTAGTG 
GGACTGAGTG TGGATAGTCC CGGAGCGGAT AGCCCGCAGC GCAAGCAGAG CGTGGTCAAG 
ACCCTCAGCG GCAGCTATGT CAACATATGC CCCCTCACGG GTGGTGAGTC CAGCTATGGC 
CTGAAGCTCG GACCCTCGCA GATCCATCCC AAGGGCTACC GCCTGACCAC CGCGAGGTAC 
GGCGAGCTGA AGATGGGCTT CCTGAAGATC AAGGGCAACG TAGAGGTGGA GCTAATCTGC 
GCCCGTAACA TTGTCAACGA GGACTGCGAA ACTCCGCCGG ACACGTACGT GAAGTGCTAC 
ATCAAGGACG GGGACCGCCT GCGCCACAAG AAGAAGACGC GTGTGGTGCG CCACGCGGCG 
GAACCGCTCT ACCGCCAGAC CATTAAGTAC CAGAGCTCGG ACGTCTTTGG CCGCAACATC 
GTCATCATGG TGTGGCAGCG GTGCGTGGGC TTCGAACACA ACCAAGGACT GGGCGGCACG 
GAGGTGAATC TGGACAAGCT GAGCATCGGC CAGCCCATCA GCGGATGGTA CCCACTTTTC 
CCGATGCACA GCTACGGAGG CTCCGACTCG GACAACTCTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261340.1
CDS	985..4929
Translation	MLPTNVMSFMKKMVATDEQTSERQQHAADSGGGASQTGALNKMKATISSSLLTVTDKVNKMSPRPSLVPTDADTSGSYGSPSYQSQQQQQQDYGSSLNNNNNNSSGGGTATTGGGGGGAAAKQEPGRRAGACRVCLKSFKPDDYHKTCFECQQRVCEDCASYSKLDEHEDATMWRCSVCRRKMASRVCIPQDSTDSMLDVPVLEALQRRHSDAKLGSSTQTLAPSNGAALAPPRSPELRRHSDVSPASLKELERQLKGGRSMAPSRSNSPPRGEGPDGQPPFGAPLSRMQSRRGSRVARQHSYDDDMKTGPVGGSMGGGGPALGLGADGAGLGIPTMPRRKSAYDVFAPGLTQGGATPATQLQRSPGEGGIMPPIQLPGSRRPSFRVPHPSEDIQNDDSPGSPDKGSPVLTVDDDRRMRRRGSQLPDIAMLQNRGALPAVPPSSIPPPMASFTGPNLEDLEAPRRQTSMDGEAIRIVIHDVDSGPICASKRRIILRRDPTDKAHRTRGFGMRVVGGKTGADGRLFAYIVWTVPGGAAEKNGLQQGDKILEWNGMSLIDRSFEEVCSIMDRTGDVVELLVEHATDFRMCDLFDENLPPGNAGGQSGPRRSGDGPVGLGLIAEPETTTDKSPASPTRRKLPKTPEQIAREKLVTGRVQIQVWYHAERSELVVSLMAGDDLAPRDEAYGHGNLPEAYAKVRILPKCGDGSVQQTEVSRPTQNPIWNATLTFGHVKADTLMVRYIDIQLWDLVPHTESIFLGECSIELQQAFLDDQAIWCRLEDTKGLRGISISKSPSVSPRGSIAAGAGAPSGDVTRLLRRDYNMQRSNSDDVDSIGDGTSLLHPDHAWIAGSRRGSSQSETMEVEVYQLGKDFSRSLPGSRRSSFQDAEKNRLEEDAMATPPTSYLVGRRRSSVARRDPDEILKSLKAVRGELGRTMSLGTEQHKRMGSRRASRVGLPSGPSSRKSSVLDLSQQHQQQLQQLQQLQLQQQQYLQQQLPMGVGNADTSPPSEDEDKRYRPRWKGSVSQLPPQSPKQALSRLKQAASASNVAQDTQRQGQLLTTNPLQQRKSSMFQLGESQASPPTTTTTHLQRKGSMYVAKVTETPPHGTPTRKGSVYQRSVVGLSVDSPGADSPQRKQSVVKTLSGSYVNICPLTGGESSYGLKLGPSQIHPKGYRLTTARYGELKMGFLKIKGNVEVELICARNIVNEDCETPPDTYVKCYIKDGDRLRHKKKTRVVRHAAEPLYRQTIKYQSSDVFGRNIVIMVWQRCVGFEHNQGLGGTEVNLDKLSIGQPISGWYPLFPMHSYGGSDSDNSP

Target ORF information:

RefSeq Version	NM_001274411.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fife, transcript variant D (Fife), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274411.1

ORF Insert Sequence:

ATGTTGCCAA CAAATGTGAT GTCATTCATG AAAAAGATGG TGGCCACCGA CGAGCAGACC 
AGCGAACGGC AGCAGCATGC AGCGGACTCG GGAGGAGGGG CCTCCCAAAC GGGGGCCCTG 
AACAAGATGA AGGCTACGAT TTCCTCCTCG CTGCTCACCG TCACAGATAA AGTTAATAAA 
ATGTCGCCAC GCCCCTCGCT GGTGCCGACG GATGCGGATA CCAGCGGCAG TTACGGCTCG 
CCCAGCTATC AAAGCCAGCA GCAGCAGCAG CAGGACTACG GCAGCAGTCT GAATAACAAC 
AATAACAACA GCAGCGGCGG CGGCACGGCG ACAACGGGCG GCGGTGGAGG TGGGGCGGCG 
GCCAAACAGG AGCCAGGACG AAGGGCCGGA GCCTGCCGGG TGTGTCTGAA GTCCTTCAAG 
CCGGACGACT ACCACAAGAC GTGCTTCGAG TGCCAGCAGC GAGTGTGCGA GGACTGCGCC 
AGCTACAGCA AGCTGGACGA GCACGAGGAT GCGACCATGT GGCGCTGCAG CGTGTGCCGC 
CGGAAAATGG CCTCGCGTGT GTGCATTCCG CAGGACTCGA CGGACTCGAT GCTGGACGTT 
CCGGTGCTGG AGGCACTGCA GCGGCGACAC TCGGACGCCA AGCTGGGCAG CAGCACGCAG 
ACCCTGGCCC CCAGCAATGG AGCAGCCCTT GCCCCGCCGC GAAGTCCCGA ACTGCGACGC 
CACTCGGATG TTTCGCCAGC CTCGCTGAAG GAGTTGGAGC GGCAACTGAA AGGCGGCCGC 
AGCATGGCGC CCAGTCGATC CAATAGTCCG CCGAGGGGCG AGGGCCCAGA TGGACAGCCC 
CCCTTCGGTG CGCCCCTGTC GCGCATGCAG TCGCGAAGGG GGTCGCGGGT GGCACGGCAG 
CACAGCTACG ATGACGACAT GAAGACCGGA CCCGTGGGTG GCAGCATGGG CGGTGGTGGT 
CCGGCGCTGG GTTTGGGTGC GGATGGAGCG GGCCTCGGCA TTCCGACCAT GCCACGCAGG 
AAGTCCGCCT ATGATGTGTT CGCCCCCGGG CTGACGCAAG GTGGTGCCAC TCCTGCCACG 
CAGCTGCAGA GATCGCCCGG CGAGGGTGGC ATCATGCCGC CCATCCAGCT GCCCGGATCC 
AGGCGACCCT CGTTCCGGGT GCCACATCCC TCGGAGGACA TCCAGAACGA CGACTCGCCC 
GGCTCGCCGG ATAAGGGCAG CCCCGTCCTG ACCGTCGACG ATGACCGGCG GATGCGGCGA 
CGCGGATCGC AGCTACCCGA TATAGCGATG CTGCAGAATC GTGGTGCCCT GCCTGCGGTT 
CCGCCCTCCT CGATTCCGCC GCCCATGGCT TCATTTACGG GCCCCAATCT GGAGGATTTG 
GAGGCGCCGC GACGGCAAAC ATCAATGGAC GGCGAGGCCA TACGGATCGT TATTCACGAC 
GTGGATTCGG GGCCGATTTG TGCATCTAAG CGGCGCATCA TTCTGCGCCG AGATCCCACG 
GACAAGGCGC ACCGCACACG TGGCTTTGGA ATGCGCGTCG TCGGCGGAAA AACAGGAGCC 
GATGGCCGTC TCTTCGCCTA CATTGTGTGG ACCGTTCCAG GAGGAGCGGC CGAGAAGAAT 
GGCCTGCAGC AGGGCGACAA GATACTCGAG TGGAATGGAA TGTCGCTGAT CGACCGAAGT 
TTCGAGGAGG TGTGCTCCAT CATGGACCGA ACCGGAGACG TTGTGGAGCT TCTGGTGGAG 
CATGCCACGG ACTTCCGGAT GTGCGATCTC TTCGATGAGA ACCTACCGCC CGGCAATGCC 
GGTGGCCAAA GCGGACCTCG GAGATCCGGA GACGGTCCCG TCGGACTGGG CCTCATAGCG 
GAACCCGAAA CCACCACAGA CAAATCACCA GCATCGCCAA CTAGACGGAA ATTACCAAAA 
ACTCCGGAGC AAATTGCCCG CGAGAAGCTG GTCACCGGAA GGGTTCAGAT CCAGGTTTGG 
TACCATGCGG AGCGCAGCGA GCTGGTCGTC TCCCTGATGG CCGGCGACGA CCTGGCACCG 
CGGGATGAGG CCTACGGACA TGGCAATCTG CCCGAGGCGT ACGCCAAGGT CCGCATTCTG 
CCGAAATGTG GAGATGGCAG CGTGCAACAA ACGGAGGTCA GCCGACCCAC ACAGAATCCC 
ATTTGGAACG CTACACTGAC CTTCGGCCAT GTGAAGGCCG ACACCTTGAT GGTTCGCTAC 
ATAGACATTC AGCTGTGGGA CCTGGTACCC CACACCGAGT CCATTTTCCT GGGCGAGTGC 
AGCATCGAGC TGCAGCAGGC CTTTCTCGAT GACCAGGCAA TATGGTGCCG CTTGGAGGAT 
ACGAAGGGGC TGCGTGGCAT AAGCATTAGC AAATCACCGA GTGTATCGCC ACGTGGCTCG 
ATTGCAGCCG GCGCAGGGGC GCCCAGTGGC GATGTGACCC GGTTGCTGCG TCGCGACTAC 
AATATGCAGC GCTCCAACTC CGATGACGTA GACTCCATCG GAGATGGAAC TTCACTGCTG 
CATCCGGATC ACGCCTGGAT AGCTGGCTCG CGACGCGGTT CCTCTCAGTC GGAAACCATG 
GAGGTGGAGG TCTACCAGCT GGGCAAGGAC TTCTCGCGCT CATTGCCGGG TTCGCGTCGA 
TCGAGCTTCC AGGATGCCGA AAAGAATCGG CTGGAGGAGG ACGCCATGGC CACGCCGCCC 
ACCTCTTATC TGGTGGGCCG GCGCCGTTCG TCCGTGGCTC GGCGCGATCC GGATGAGATC 
TTGAAATCCT TGAAGGCTGT CCGCGGTGAG CTGGGGCGCA CCATGAGTCT GGGTACCGAG 
CAGCACAAGC GCATGGGATC GAGACGTGCC AGTCGCGTTG GATTGCCGAG CGGACCGAGT 
AGCCGGAAGA GTTCCGTCTT AGATTTGTCG CAGCAGCATC AGCAGCAGTT GCAGCAGCTG 
CAGCAGTTGC AGTTGCAGCA GCAGCAGTAC CTGCAGCAGC AGCTGCCCAT GGGAGTGGGC 
AATGCCGACA CCAGTCCACC GTCCGAAGAC GAGGATAAGC GCTACAGGCC GCGCTGGAAG 
GGATCGGTCA GCCAGTTGCC GCCGCAGAGT CCCAAGCAGG CGCTATCCCG GCTCAAGCAG 
GCCGCATCCG CGTCGAACGT GGCCCAGGAC ACGCAGCGAC AGGGCCAACT GCTGACCACC 
AACCCACTGC AGCAACGCAA GAGCAGCATG TTCCAACTGG GCGAGTCCCA GGCATCGCCC 
CCAACGACCA CCACCACCCA CTTGCAGCGG AAGGGCAGCA TGTACGTCGC CAAGGTCACC 
GAGACACCGC CCCACGGTAC GCCCACCCGA AAGGGCAGCG TCTACCAGAG AAGCGTAGTG 
GGACTGAGTG TGGATAGTCC CGGAGCGGAT AGCCCGCAGC GCAAGCAGAG CGTGGTCAAG 
ACCCTCAGCG GCAGCTATGT CAACATATGC CCCCTCACGG GTGGTGAGTC CAGCTATGGC 
CTGAAGCTCG GACCCTCGCA GATCCATCCC AAGGGCTACC GCCTGACCAC CGCGAGGTAC 
GGCGAGCTGA AGATGGGCTT CCTGAAGATC AAGGGCAACG TAGAGGTGGA GCTAATCTGC 
GCCCGTAACA TTGTCAACGA GGACTGCGAA ACTCCGCCGG ACACGTACGT GAAGTGCTAC 
ATCAAGGACG GGGACCGCCT GCGCCACAAG AAGAAGACGC GTGTGGTGCG CCACGCGGCG 
GAACCGCTCT ACCGCCAGAC CATTAAGTAC CAGAGCTCGG ACGTCTTTGG CCGCAACATC 
GTCATCATGG TGTGGCAGCG GTGCGTGGGC TTCGAACACA ACCAAGGACT GGGCGGCACG 
GAGGTGAATC TGGACAAGCT GAGCATCGGC CAGCCCATCA GCGGATGGTA CCCACTTTTC 
CCGATGCACA GCTACGGAGG CTCCGACTCG GACAACTCTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fife cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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