Gale cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gale
Entrez Gene ID	38076
Full Name	UDP-galactose 4'-epimerase
Synonyms	CG12030,Dmel\CG12030,GALE,dGALE
General protein information	Preferred Names UDP-galactose 4'-epimerase Names CG12030 gene product from transcript CG12030-RA CG12030-PA CG12030-PB Gale-PA Gale-PB UDP-gal 4' epimerase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61C8-61C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259608.2	NP_001246537.1	UDP-galactose 4'-epimerase, isoform B
NM_138200.4	NP_612044.1	UDP-galactose 4'-epimerase, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	gale	NP_001035389.1
Canis lupus familiaris (dog)	GALE	XP_003638951.1
Rattus norvegicus (Norway rat)	Gale	NP_542961.2
Arabidopsis thaliana (thale cress)	UGE2	NP_194123.1
Oryza sativa (rice)	Os05g0595100	NP_001056512.1
Homo sapiens (human)	GALE	NP_001008217.1
Gallus gallus (chicken)	GALE	XP_004947951.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012261	XP_320278.2
Mus musculus (house mouse)	Gale	NP_848476.1
Drosophila melanogaster (fruit fly)	Gale	NP_001246537.1
Caenorhabditis elegans (roundworm)	gale-1	NP_001021051.1
Schizosaccharomyces pombe (fission yeast)	uge1	NP_596043.1
Pan troglodytes (chimpanzee)	GALE	XP_003307924.2
	GALE	XP_001104253.1
Bos taurus (cattle)	GALE	NP_001193137.1
Arabidopsis thaliana (thale cress)	UGE5	NP_192834.1
Arabidopsis thaliana (thale cress)	RHD1	NP_176625.1
Xenopus tropicalis (tropical clawed frog)	gale	NP_001006762.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results demonstrate that both UDP-gal and UDP-galNAc activities of dGALE are required for Drosophila survival, although distinct roles for each activity can be seen in specific windows of developmental time or in response to a galactose challenge.
Title: UDP-galactose 4'-epimerase activities toward UDP-Gal and UDP-GalNAc play different roles in the development of Drosophila melanogaster.

Drosophila with partial GALE loss survive in the absence of galactose but succumb in development if exposed to dietary galactose.
Title: UDP-galactose 4' epimerase (GALE) is essential for development of Drosophila melanogaster.

The following Gale gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gale cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17078	NM_001259608.2 Latest version!	Drosophila melanogaster UDP-galactose 4'-epimerase (Gale), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17078	NM_138200.4 Latest version!	Drosophila melanogaster UDP-galactose 4'-epimerase (Gale), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gale ( NM_001259608.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246537.1 Drosophila melanogaster UDP-galactose 4'-epimerase (Gale), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17078
Clone ID Related Accession (Same CDS sequence)	NM_001259608.2 , NM_138200.4
Accession Version	NM_001259608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	UDP-galactose 4'-epimerase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259608.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCCAC CCACGGTCCT GGTCACCGGA GGAGCCGGCT ACATTGGCTC CCACACGGTT 
CTGGAGATGC TCAATGCGGG CTACAACGTC ATCTGCGTGG ACAACCTGTG CAACGCCTAC 
AGCAGCGGGG CCAAACTGCC GGAGGCCCTC AGCCGGGTGC AGGAAATCAC CGGCAAGAAG 
GTCAACTTCT ATAGAGTGGA CATCACGGAC AGGGAGCAGG TGCGCTCCGT CTTCCAGGAG 
CACAAAATCG ACATGGTGGC CCACTTTGCC GCCCTGAAGG CCGTGGGTGA GTCCTGCCGC 
ATACCTTTGC AGTACTACCA CAACAACATG ACCGGCACCA ATGTCCTGCT GGAGGCGATG 
GCCGACAACA ATGTCTTCAA GTTCGTGTAC AGCTCCAGCG CCACCGTATA CGGTGAGCCA 
AAGTTCCTGC CCGTGACCGA GGAGCATCCC ACGGGCAACT GTACATCGCC CTACGGCAAG 
ACTAAATATT TCACCGAGGA GATTCTCAAG GATCTGTGCA AGTCTGACAA GCGTTGGGCA 
GTGGTTTCGC TGCGGTACTT CAACCCGGTG GGTGCCCACA TCAGTGGTCG GATTGGCGAG 
GATCCGAACG GCGAGCCCAA CAACCTGATG CCCTACATCG CACAGGTGGC CGTGGGTCGA 
CGTCCCAGCC TGAGTGTCTA CGGCAGCGAC TTTCCCACGC ACGACGGCAC CGGCGTGCGC 
GATTACATCC ACATCGTGGA CCTGGCCGAG GGACACGTAA AGGCGTTGGA CAAGCTGCGC 
AACATCGCGG AGACGGGCTT TTTCGCCTAC AATTTGGGCA CCGGCGTGGG CTACTCGGTG 
CTGGACATGG TGAAGGCGTT TGAGAAGGCC TCCGGCAAGA AGGTCAACTA CACTCTGGTG 
GACCGACGGT CCGGAGATGT GGCCACTTGC TATGCGGACG CCACGTTGGC GGATAAGAAG 
CTGGGCTGGA AGGCCGAGCG TGGCATCGAC AAGATGTGCG AGGACACGTG GCGCTGGCAG 
AGCCAGAACC CAAACGGGTA TGCCAACAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246537.1
CDS	280..1332
Translation	MAPPTVLVTGGAGYIGSHTVLEMLNAGYNVICVDNLCNAYSSGAKLPEALSRVQEITGKKVNFYRVDITDREQVRSVFQEHKIDMVAHFAALKAVGESCRIPLQYYHNNMTGTNVLLEAMADNNVFKFVYSSSATVYGEPKFLPVTEEHPTGNCTSPYGKTKYFTEEILKDLCKSDKRWAVVSLRYFNPVGAHISGRIGEDPNGEPNNLMPYIAQVAVGRRPSLSVYGSDFPTHDGTGVRDYIHIVDLAEGHVKALDKLRNIAETGFFAYNLGTGVGYSVLDMVKAFEKASGKKVNYTLVDRRSGDVATCYADATLADKKLGWKAERGIDKMCEDTWRWQSQNPNGYANK

Target ORF information:

RefSeq Version	NM_001259608.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster UDP-galactose 4'-epimerase (Gale), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259608.2

ORF Insert Sequence:

ATGGCCCCAC CCACGGTCCT GGTCACCGGA GGAGCCGGCT ACATTGGCTC CCACACGGTT 
CTGGAGATGC TCAATGCGGG CTACAACGTC ATCTGCGTGG ACAACCTGTG CAACGCCTAC 
AGCAGCGGGG CCAAACTGCC GGAGGCCCTC AGCCGGGTGC AGGAAATCAC CGGCAAGAAG 
GTCAACTTCT ATAGAGTGGA CATCACGGAC AGGGAGCAGG TGCGCTCCGT CTTCCAGGAG 
CACAAAATCG ACATGGTGGC CCACTTTGCC GCCCTGAAGG CCGTGGGTGA GTCCTGCCGC 
ATACCTTTGC AGTACTACCA CAACAACATG ACCGGCACCA ATGTCCTGCT GGAGGCGATG 
GCCGACAACA ATGTCTTCAA GTTCGTGTAC AGCTCCAGCG CCACCGTATA CGGTGAGCCA 
AAGTTCCTGC CCGTGACCGA GGAGCATCCC ACGGGCAACT GTACATCGCC CTACGGCAAG 
ACTAAATATT TCACCGAGGA GATTCTCAAG GATCTGTGCA AGTCTGACAA GCGTTGGGCA 
GTGGTTTCGC TGCGGTACTT CAACCCGGTG GGTGCCCACA TCAGTGGTCG GATTGGCGAG 
GATCCGAACG GCGAGCCCAA CAACCTGATG CCCTACATCG CACAGGTGGC CGTGGGTCGA 
CGTCCCAGCC TGAGTGTCTA CGGCAGCGAC TTTCCCACGC ACGACGGCAC CGGCGTGCGC 
GATTACATCC ACATCGTGGA CCTGGCCGAG GGACACGTAA AGGCGTTGGA CAAGCTGCGC 
AACATCGCGG AGACGGGCTT TTTCGCCTAC AATTTGGGCA CCGGCGTGGG CTACTCGGTG 
CTGGACATGG TGAAGGCGTT TGAGAAGGCC TCCGGCAAGA AGGTCAACTA CACTCTGGTG 
GACCGACGGT CCGGAGATGT GGCCACTTGC TATGCGGACG CCACGTTGGC GGATAAGAAG 
CTGGGCTGGA AGGCCGAGCG TGGCATCGAC AAGATGTGCG AGGACACGTG GCGCTGGCAG 
AGCCAGAACC CAAACGGGTA TGCCAACAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gale ( NM_138200.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_612044.1 Drosophila melanogaster UDP-galactose 4'-epimerase (Gale), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17078
Clone ID Related Accession (Same CDS sequence)	NM_001259608.2 , NM_138200.4
Accession Version	NM_138200.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	UDP-galactose 4'-epimerase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_138200.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCCAC CCACGGTCCT GGTCACCGGA GGAGCCGGCT ACATTGGCTC CCACACGGTT 
CTGGAGATGC TCAATGCGGG CTACAACGTC ATCTGCGTGG ACAACCTGTG CAACGCCTAC 
AGCAGCGGGG CCAAACTGCC GGAGGCCCTC AGCCGGGTGC AGGAAATCAC CGGCAAGAAG 
GTCAACTTCT ATAGAGTGGA CATCACGGAC AGGGAGCAGG TGCGCTCCGT CTTCCAGGAG 
CACAAAATCG ACATGGTGGC CCACTTTGCC GCCCTGAAGG CCGTGGGTGA GTCCTGCCGC 
ATACCTTTGC AGTACTACCA CAACAACATG ACCGGCACCA ATGTCCTGCT GGAGGCGATG 
GCCGACAACA ATGTCTTCAA GTTCGTGTAC AGCTCCAGCG CCACCGTATA CGGTGAGCCA 
AAGTTCCTGC CCGTGACCGA GGAGCATCCC ACGGGCAACT GTACATCGCC CTACGGCAAG 
ACTAAATATT TCACCGAGGA GATTCTCAAG GATCTGTGCA AGTCTGACAA GCGTTGGGCA 
GTGGTTTCGC TGCGGTACTT CAACCCGGTG GGTGCCCACA TCAGTGGTCG GATTGGCGAG 
GATCCGAACG GCGAGCCCAA CAACCTGATG CCCTACATCG CACAGGTGGC CGTGGGTCGA 
CGTCCCAGCC TGAGTGTCTA CGGCAGCGAC TTTCCCACGC ACGACGGCAC CGGCGTGCGC 
GATTACATCC ACATCGTGGA CCTGGCCGAG GGACACGTAA AGGCGTTGGA CAAGCTGCGC 
AACATCGCGG AGACGGGCTT TTTCGCCTAC AATTTGGGCA CCGGCGTGGG CTACTCGGTG 
CTGGACATGG TGAAGGCGTT TGAGAAGGCC TCCGGCAAGA AGGTCAACTA CACTCTGGTG 
GACCGACGGT CCGGAGATGT GGCCACTTGC TATGCGGACG CCACGTTGGC GGATAAGAAG 
CTGGGCTGGA AGGCCGAGCG TGGCATCGAC AAGATGTGCG AGGACACGTG GCGCTGGCAG 
AGCCAGAACC CAAACGGGTA TGCCAACAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612044.1
CDS	499..1551
Translation	MAPPTVLVTGGAGYIGSHTVLEMLNAGYNVICVDNLCNAYSSGAKLPEALSRVQEITGKKVNFYRVDITDREQVRSVFQEHKIDMVAHFAALKAVGESCRIPLQYYHNNMTGTNVLLEAMADNNVFKFVYSSSATVYGEPKFLPVTEEHPTGNCTSPYGKTKYFTEEILKDLCKSDKRWAVVSLRYFNPVGAHISGRIGEDPNGEPNNLMPYIAQVAVGRRPSLSVYGSDFPTHDGTGVRDYIHIVDLAEGHVKALDKLRNIAETGFFAYNLGTGVGYSVLDMVKAFEKASGKKVNYTLVDRRSGDVATCYADATLADKKLGWKAERGIDKMCEDTWRWQSQNPNGYANK

Target ORF information:

RefSeq Version	NM_138200.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster UDP-galactose 4'-epimerase (Gale), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138200.4

ORF Insert Sequence:

ATGGCCCCAC CCACGGTCCT GGTCACCGGA GGAGCCGGCT ACATTGGCTC CCACACGGTT 
CTGGAGATGC TCAATGCGGG CTACAACGTC ATCTGCGTGG ACAACCTGTG CAACGCCTAC 
AGCAGCGGGG CCAAACTGCC GGAGGCCCTC AGCCGGGTGC AGGAAATCAC CGGCAAGAAG 
GTCAACTTCT ATAGAGTGGA CATCACGGAC AGGGAGCAGG TGCGCTCCGT CTTCCAGGAG 
CACAAAATCG ACATGGTGGC CCACTTTGCC GCCCTGAAGG CCGTGGGTGA GTCCTGCCGC 
ATACCTTTGC AGTACTACCA CAACAACATG ACCGGCACCA ATGTCCTGCT GGAGGCGATG 
GCCGACAACA ATGTCTTCAA GTTCGTGTAC AGCTCCAGCG CCACCGTATA CGGTGAGCCA 
AAGTTCCTGC CCGTGACCGA GGAGCATCCC ACGGGCAACT GTACATCGCC CTACGGCAAG 
ACTAAATATT TCACCGAGGA GATTCTCAAG GATCTGTGCA AGTCTGACAA GCGTTGGGCA 
GTGGTTTCGC TGCGGTACTT CAACCCGGTG GGTGCCCACA TCAGTGGTCG GATTGGCGAG 
GATCCGAACG GCGAGCCCAA CAACCTGATG CCCTACATCG CACAGGTGGC CGTGGGTCGA 
CGTCCCAGCC TGAGTGTCTA CGGCAGCGAC TTTCCCACGC ACGACGGCAC CGGCGTGCGC 
GATTACATCC ACATCGTGGA CCTGGCCGAG GGACACGTAA AGGCGTTGGA CAAGCTGCGC 
AACATCGCGG AGACGGGCTT TTTCGCCTAC AATTTGGGCA CCGGCGTGGG CTACTCGGTG 
CTGGACATGG TGAAGGCGTT TGAGAAGGCC TCCGGCAAGA AGGTCAACTA CACTCTGGTG 
GACCGACGGT CCGGAGATGT GGCCACTTGC TATGCGGACG CCACGTTGGC GGATAAGAAG 
CTGGGCTGGA AGGCCGAGCG TGGCATCGAC AAGATGTGCG AGGACACGTG GCGCTGGCAG 
AGCCAGAACC CAAACGGGTA TGCCAACAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gale cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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