miple1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	miple1
Entrez Gene ID	38047
Full Name	midkine and pleiotrophin 1
Synonyms	CG1221,Dmel\CG1221,Miple,anon-EST:Posey203,miple
General protein information	Preferred Names midkine and pleiotrophin 1 Names CG1221 gene product from transcript CG1221-RC CG1221-PA CG1221-PB CG1221-PC miple miple1-PA miple1-PB miple1-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61B3-61B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_138178.3	NP_612022.1	midkine and pleiotrophin 1, isoform A
NM_001274268.1	NP_001261197.1	midkine and pleiotrophin 1, isoform C
NM_206225.2	NP_995947.1	midkine and pleiotrophin 1, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	miple	NP_001261197.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005674	XP_315691.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data suggest that Alk is not activated by MDK/PTN related growth factors Miple1 and Miple2 in vivo.
Title: The Drosophila midkine/pleiotrophin homologues Miple1 and Miple2 affect adult lifespan but are dispensable for alk signaling during embryonic gut formation.

One of the roles of Nmo during ommatial rotation is to repress miple expression, which may in turn affect the dynamics in E-cadherin-beta-catenin complexes.
Title: Nemo regulates cell dynamics and represses the expression of miple, a midkine/pleiotrophin cytokine, during ommatidial rotation.

overexpression of miple (a midkine and pleiotrophin heparin-binding growth factor), in the mesoderm led to abnormal scattered MAPK activation, a phenotype that might explain the abnormal mesoderm spreading
Title: Post-transcriptional repression of the Drosophila midkine and pleiotrophin homolog miple by HOW is essential for correct mesoderm spreading.

Expression can be found at high levels in the central nervous system.
Title: Miple1 and miple2 encode a family of MK/PTN homologues in Drosophila melanogaster.

The following miple1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the miple1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17052	NM_206225.2 Latest version!	Drosophila melanogaster midkine and pleiotrophin 1 (miple1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17052	NM_001274268.1 Latest version!	Drosophila melanogaster midkine and pleiotrophin 1 (miple1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17052	NM_138178.3 Latest version!	Drosophila melanogaster midkine and pleiotrophin 1 (miple1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

miple1 ( NM_138178.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_612022.1 Drosophila melanogaster midkine and pleiotrophin 1 (miple1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17052
Clone ID Related Accession (Same CDS sequence)	NM_138178.3 , NM_001274268.1 , NM_206225.2
Accession Version	NM_138178.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	midkine and pleiotrophin 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_138178.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAATTA ATTGCAACGC GCTTTTCCTG GCATCGCTAG TGACTTGGTC GGGGGTGATG 
TGCAGCACGG TACTGGGCAC CACAGAAGGC CAGGAAACAC CTTTGGCCCT TCCTGTGGCG 
GAGCAGACGC AGCCCACTAC AGCCATACAA GGAGAAGTAT GGGAGGAGGA CGATCACGAA 
GTGCTTATAA GAAATGAACG TGGCACCAAG AGCGACGGTC TGTCTTGTCG ATATGGCAAA 
AATCCGTGGA CTGAGTGTGA CACCAAAACC AATACGCGTT CAAGAACATT GACATTAAAG 
AAGGGCGACC CGGCATGCGA CCAAACGCGA ACCATTCAGA AGAAATGTAA AAAAGCCTGT 
CGATATGAAA AGGGATCTTG GTCGGAATGT GCAACCGGAC AAATGACTAG AGCTGATAAG 
TTGAAAGCGA GCAGTGATCC GTCCTGCGAA GCCACGCGGG TCATCAAGAA GAACTGCAAG 
CCCGGAAAGT CCAAGGACAA AAGTGCCAAG GAGCAGCGGA AGAACAAAGA TAAAGCTGCT 
CGCAAAGGAC GCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612022.1
CDS	130..687
Translation	MRINCNALFLASLVTWSGVMCSTVLGTTEGQETPLALPVAEQTQPTTAIQGEVWEEDDHEVLIRNERGTKSDGLSCRYGKNPWTECDTKTNTRSRTLTLKKGDPACDQTRTIQKKCKKACRYEKGSWSECATGQMTRADKLKASSDPSCEATRVIKKNCKPGKSKDKSAKEQRKNKDKAARKGRV

Target ORF information:

RefSeq Version	NM_138178.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster midkine and pleiotrophin 1 (miple1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138178.3

ORF Insert Sequence:

ATGAGAATTA ATTGCAACGC GCTTTTCCTG GCATCGCTAG TGACTTGGTC GGGGGTGATG 
TGCAGCACGG TACTGGGCAC CACAGAAGGC CAGGAAACAC CTTTGGCCCT TCCTGTGGCG 
GAGCAGACGC AGCCCACTAC AGCCATACAA GGAGAAGTAT GGGAGGAGGA CGATCACGAA 
GTGCTTATAA GAAATGAACG TGGCACCAAG AGCGACGGTC TGTCTTGTCG ATATGGCAAA 
AATCCGTGGA CTGAGTGTGA CACCAAAACC AATACGCGTT CAAGAACATT GACATTAAAG 
AAGGGCGACC CGGCATGCGA CCAAACGCGA ACCATTCAGA AGAAATGTAA AAAAGCCTGT 
CGATATGAAA AGGGATCTTG GTCGGAATGT GCAACCGGAC AAATGACTAG AGCTGATAAG 
TTGAAAGCGA GCAGTGATCC GTCCTGCGAA GCCACGCGGG TCATCAAGAA GAACTGCAAG 
CCCGGAAAGT CCAAGGACAA AAGTGCCAAG GAGCAGCGGA AGAACAAAGA TAAAGCTGCT 
CGCAAAGGAC GCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

miple1 ( NM_001274268.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261197.1 Drosophila melanogaster midkine and pleiotrophin 1 (miple1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17052
Clone ID Related Accession (Same CDS sequence)	NM_138178.3 , NM_001274268.1 , NM_206225.2
Accession Version	NM_001274268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	midkine and pleiotrophin 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAATTA ATTGCAACGC GCTTTTCCTG GCATCGCTAG TGACTTGGTC GGGGGTGATG 
TGCAGCACGG TACTGGGCAC CACAGAAGGC CAGGAAACAC CTTTGGCCCT TCCTGTGGCG 
GAGCAGACGC AGCCCACTAC AGCCATACAA GGAGAAGTAT GGGAGGAGGA CGATCACGAA 
GTGCTTATAA GAAATGAACG TGGCACCAAG AGCGACGGTC TGTCTTGTCG ATATGGCAAA 
AATCCGTGGA CTGAGTGTGA CACCAAAACC AATACGCGTT CAAGAACATT GACATTAAAG 
AAGGGCGACC CGGCATGCGA CCAAACGCGA ACCATTCAGA AGAAATGTAA AAAAGCCTGT 
CGATATGAAA AGGGATCTTG GTCGGAATGT GCAACCGGAC AAATGACTAG AGCTGATAAG 
TTGAAAGCGA GCAGTGATCC GTCCTGCGAA GCCACGCGGG TCATCAAGAA GAACTGCAAG 
CCCGGAAAGT CCAAGGACAA AAGTGCCAAG GAGCAGCGGA AGAACAAAGA TAAAGCTGCT 
CGCAAAGGAC GCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261197.1
CDS	117..674
Translation	MRINCNALFLASLVTWSGVMCSTVLGTTEGQETPLALPVAEQTQPTTAIQGEVWEEDDHEVLIRNERGTKSDGLSCRYGKNPWTECDTKTNTRSRTLTLKKGDPACDQTRTIQKKCKKACRYEKGSWSECATGQMTRADKLKASSDPSCEATRVIKKNCKPGKSKDKSAKEQRKNKDKAARKGRV

Target ORF information:

RefSeq Version	NM_001274268.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster midkine and pleiotrophin 1 (miple1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274268.1

ORF Insert Sequence:

ATGAGAATTA ATTGCAACGC GCTTTTCCTG GCATCGCTAG TGACTTGGTC GGGGGTGATG 
TGCAGCACGG TACTGGGCAC CACAGAAGGC CAGGAAACAC CTTTGGCCCT TCCTGTGGCG 
GAGCAGACGC AGCCCACTAC AGCCATACAA GGAGAAGTAT GGGAGGAGGA CGATCACGAA 
GTGCTTATAA GAAATGAACG TGGCACCAAG AGCGACGGTC TGTCTTGTCG ATATGGCAAA 
AATCCGTGGA CTGAGTGTGA CACCAAAACC AATACGCGTT CAAGAACATT GACATTAAAG 
AAGGGCGACC CGGCATGCGA CCAAACGCGA ACCATTCAGA AGAAATGTAA AAAAGCCTGT 
CGATATGAAA AGGGATCTTG GTCGGAATGT GCAACCGGAC AAATGACTAG AGCTGATAAG 
TTGAAAGCGA GCAGTGATCC GTCCTGCGAA GCCACGCGGG TCATCAAGAA GAACTGCAAG 
CCCGGAAAGT CCAAGGACAA AAGTGCCAAG GAGCAGCGGA AGAACAAAGA TAAAGCTGCT 
CGCAAAGGAC GCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

miple1 ( NM_206225.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995947.1 Drosophila melanogaster midkine and pleiotrophin 1 (miple1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17052
Clone ID Related Accession (Same CDS sequence)	NM_138178.3 , NM_001274268.1 , NM_206225.2
Accession Version	NM_206225.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	midkine and pleiotrophin 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206225.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAATTA ATTGCAACGC GCTTTTCCTG GCATCGCTAG TGACTTGGTC GGGGGTGATG 
TGCAGCACGG TACTGGGCAC CACAGAAGGC CAGGAAACAC CTTTGGCCCT TCCTGTGGCG 
GAGCAGACGC AGCCCACTAC AGCCATACAA GGAGAAGTAT GGGAGGAGGA CGATCACGAA 
GTGCTTATAA GAAATGAACG TGGCACCAAG AGCGACGGTC TGTCTTGTCG ATATGGCAAA 
AATCCGTGGA CTGAGTGTGA CACCAAAACC AATACGCGTT CAAGAACATT GACATTAAAG 
AAGGGCGACC CGGCATGCGA CCAAACGCGA ACCATTCAGA AGAAATGTAA AAAAGCCTGT 
CGATATGAAA AGGGATCTTG GTCGGAATGT GCAACCGGAC AAATGACTAG AGCTGATAAG 
TTGAAAGCGA GCAGTGATCC GTCCTGCGAA GCCACGCGGG TCATCAAGAA GAACTGCAAG 
CCCGGAAAGT CCAAGGACAA AAGTGCCAAG GAGCAGCGGA AGAACAAAGA TAAAGCTGCT 
CGCAAAGGAC GCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995947.1
CDS	114..671
Translation	MRINCNALFLASLVTWSGVMCSTVLGTTEGQETPLALPVAEQTQPTTAIQGEVWEEDDHEVLIRNERGTKSDGLSCRYGKNPWTECDTKTNTRSRTLTLKKGDPACDQTRTIQKKCKKACRYEKGSWSECATGQMTRADKLKASSDPSCEATRVIKKNCKPGKSKDKSAKEQRKNKDKAARKGRV

Target ORF information:

RefSeq Version	NM_206225.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster midkine and pleiotrophin 1 (miple1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206225.2

ORF Insert Sequence:

ATGAGAATTA ATTGCAACGC GCTTTTCCTG GCATCGCTAG TGACTTGGTC GGGGGTGATG 
TGCAGCACGG TACTGGGCAC CACAGAAGGC CAGGAAACAC CTTTGGCCCT TCCTGTGGCG 
GAGCAGACGC AGCCCACTAC AGCCATACAA GGAGAAGTAT GGGAGGAGGA CGATCACGAA 
GTGCTTATAA GAAATGAACG TGGCACCAAG AGCGACGGTC TGTCTTGTCG ATATGGCAAA 
AATCCGTGGA CTGAGTGTGA CACCAAAACC AATACGCGTT CAAGAACATT GACATTAAAG 
AAGGGCGACC CGGCATGCGA CCAAACGCGA ACCATTCAGA AGAAATGTAA AAAAGCCTGT 
CGATATGAAA AGGGATCTTG GTCGGAATGT GCAACCGGAC AAATGACTAG AGCTGATAAG 
TTGAAAGCGA GCAGTGATCC GTCCTGCGAA GCCACGCGGG TCATCAAGAA GAACTGCAAG 
CCCGGAAAGT CCAAGGACAA AAGTGCCAAG GAGCAGCGGA AGAACAAAGA TAAAGCTGCT 
CGCAAAGGAC GCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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miple1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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