Hsl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsl
Entrez Gene ID	37289
Full Name	Hormone-sensitive lipase
Synonyms	A,CG11055,Dmel\CG11055,anon-56Ea,dHSL
General protein information	Preferred Names Hormone-sensitive lipase Names CG11055 gene product from transcript CG11055-RB CG11055-PA CG11055-PB CG11055-PC Hormone-sensitive lipase ortholog Hsl-PA Hsl-PB Hsl-PC hormone sensitive lipase transcription unit A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56F11-56F11

mRNA and Protein(s)

mRNA	Protein	Name
NM_137619.3	NP_611463.1	Hormone-sensitive lipase, isoform B
NM_166381.2	NP_725941.1	Hormone-sensitive lipase, isoform A
NM_166382.2	NP_725942.1	Hormone-sensitive lipase, isoform C

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	lipea	XP_003200592.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002567	XP_312370.5
Drosophila melanogaster (fruit fly)	CG11055	NP_725942.1
Caenorhabditis elegans (roundworm)	C46C11.1	NP_001041228.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hsl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13116	NM_137619.3 Latest version!	Drosophila melanogaster Hormone-sensitive lipase (Hsl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13116	NM_166382.2 Latest version!	Drosophila melanogaster Hormone-sensitive lipase (Hsl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13116	NM_166381.2 Latest version!	Drosophila melanogaster Hormone-sensitive lipase (Hsl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsl ( NM_137619.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611463.1 Drosophila melanogaster Hormone-sensitive lipase (Hsl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13116
Clone ID Related Accession (Same CDS sequence)	NM_137619.3 , NM_166381.2 , NM_166382.2
Accession Version	NM_137619.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Hormone-sensitive lipase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137619.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGACG CGGCTTCCGC CGAGCGGGCA AGCCCCCAGT TTATTGCCCT CTTCAATGAC 
AATCTGAAAC TGGGCCACGA GGACGACGGC CCACAGGCAG TCAATGGAAA GGACACCCAC 
CAGCACGTAC CCGCCGACCT GGAGGCGACC TATGGAACCT TGTACGCCGC CTGCCAGGAT 
CACGCCGCCT TCTTTGCCCG GGACCACACC GAATTCGGCC AGCGCCTGCA TGCCGCCCAC 
ATTGCCTGGC AGGACTTCAT TGTCTTGGCC AACCGACTGG TCCAGCAGAT CGATGCCTTC 
GCCCACGAGT ACGATTTTGA CGAGCAGACG CCGGGCAATG GATACAGGAG CTTCATCTAC 
GTGACCAACG CCTGCATCGC GCATGGCATT AGCATCTGCC AGCAGCTGAC AGCCACCCGT 
TCCACCATAT TCTTCCGCAA GAAGTTCTAC ATGAAGGAGG TTGAGGCCTG TTCCCAGCTG 
TTGTCGTCGC TGTGCACATG TCTGCAGTAC CTGCTGATCC TTCGCCAGTG GTCGGCTAGC 
ACGGGTGATC TGTTCGCATG CGGAAATCAC ACTGCCGAGC AGCTCTTCGA GCTGGGCGAC 
ACCATCAACC AGTATTGCTT TTACGGACGC TGCCTGGGCT TTCAGTATGG CGACTCGATA 
CGCGGAGTTC TCCGATTCCT CGGCATCAGC ATGGCCAGCT ACTCGGAGTC GTACTATTCG 
CAAGAGGGCG ACGGCCCCAT CGTTAAGACC ACACGCAGCC TGTGGACCAG CGGCAAGTAT 
CTTATGAATC CGGAACTGCG CGCCCGTCGC ATCGTTAACA TCTCGCAGAA TGCTAAGATA 
GACTTTTGCA AATCCTTTTG GTTTCTGGCC GAGTCCGAGA TGATGCACAA GCTGCCCAGC 
ATTGTGGGCA GCTCCATTAA GGTAAACCGG CTCATCGAAT TGCCAGCCGA GCCGCTAAAG 
CTGCCTCGCC GAAAGAATTT CAAGGCATCG GACAATCTCA GCAGTGACGT TAACCAAAAC 
CAAGGCGATG GTGATTTTGT GGAGATTCCC GTGCCCACGG CACATTTGGG ACCTGGTTTA 
CCGGTCTCTG TTCGCTTGCT GAGCGCGAGA CGACGTTCGG GAATGCTGGG CGAGGGTCGC 
TATCGCGGCT GGCACAAGCC CATTCCGCCA AGTCCTTCGA TTCTGTTTCA CTGCCATGGC 
GGTGGCTTTG TGGCCCAATC GTCCAAGTCC CACGAACTGT ATTTGCGCGA CTGGGCCGTG 
GCACTGGACT GTCCTATTCT CTCGGTGGAT TACAGCCTAG CTCCTGAGGC GCCATTTCCA 
CGCGCCCTGC AAGAAGTTTA CTACGCTTAC TGCTGGCTTC TGAACAATAC CGAACTCCTA 
GGCACAACAG CTGAGCGAGT TGTATGCGCA GGTGACTCGG CTGGGGCAAA TCTGTCAATT 
GGAGTAGCCC TCAAGTGCAT TGAGCAGGGA GTTCGAGTGC CAGATGGTCT GTTTCTGGCC 
TACTGCCCAA CTCTCGTCAG CTTTGTGCCC AGTCCGGCCC GCCTTCTCTG TTTGATGGAT 
CCCCTGCTGC CTTTCGGCTT CATGATGCGC TGCCTTCGTG CCTATGCCGC TCCCGCCCAG 
GAGACACTTC AGCAGAATGC CAAGCAAGTC GAGGATGCCG CCCAGATACG CAATGTCCCC 
AAGTCGAACG TGGGAAGCTT GAACTCCAGT CGCCGCACCT CGATGGCCAG AAGTCCGCTT 
TCGCCGCTGG AAGCCAGCAT GAACCCAGAT GACGAAAGCA GCGACACCTT TGCCAGTGCC 
TCAGCCTCTT ACCACAGCCA GACGGTGGAG CGTACCGATC TCCCCCACAC CGAAGGCGAC 
AACAGCTCGT GCGTCTCGTT CGAGGATGAT TCACAGCCCA TTGTGCACTA CCCAATCGAA 
ATATCAGCCG ACCCACCTAA GGATACCGCC TCTGCAGCCT ATATTGACAA TTTTCTGGAC 
AAGTATCTCA TTGACACGGC CACAATGGAG GTAACCGAAG AGACAGCTCC CGAAGCGGTG 
CAAGCGCAGG CAAATGGACA TGCGAAGATC AGTTCCGATG ATGATATTCT AGTGGAGACT 
GGTCGGGATC TTGTTGCAAT AGATACGCTG CAAGGCCGCC TGCAGGAAGC CGTCAACAAC 
ATAACCAACA CCTTGACGCG CTGCACGCAG TCCTACGAGA TTCACGGAAG CAACGTGATG 
GCTCAACAGG ATGTGCGCAA CATGGACGCA CTGATAGCAC GGAGTCCCAG CGAGGAGTTT 
GCATTCGATG TGCCTAAGGA TCCATTCCTG TCGCCGTACT GGGCCAGTGA TGAGTGGCTT 
TCCCAACTGC CGGAGACCAA AATACTTACA CTTAACATGG ACCCCTGTCT GGATGATTGC 
GTTATGTTTG CCAAGAAACT GAAGCGGCTT GGGCGACAAG TTGACTTGGA AATACTTGAG 
GGGTTGCCGC ACGGATTCCT AAATTTCACA ATGTTATCCA ACGAAGCCAT GGAGGGCTCA 
AAGAAGTGCA TCAAATCTCT ACAGACGTTG CTTCAAACCG ATTCGAAGAC CAAGAACATT 
GACAAAGCCA ACTCGATGGA CGAGGAGGAG TCCTCCAGTC CCAGTGCAAG TCTAGCCGCT 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611463.1
CDS	110..2755
Translation	MIDAASAERASPQFIALFNDNLKLGHEDDGPQAVNGKDTHQHVPADLEATYGTLYAACQDHAAFFARDHTEFGQRLHAAHIAWQDFIVLANRLVQQIDAFAHEYDFDEQTPGNGYRSFIYVTNACIAHGISICQQLTATRSTIFFRKKFYMKEVEACSQLLSSLCTCLQYLLILRQWSASTGDLFACGNHTAEQLFELGDTINQYCFYGRCLGFQYGDSIRGVLRFLGISMASYSESYYSQEGDGPIVKTTRSLWTSGKYLMNPELRARRIVNISQNAKIDFCKSFWFLAESEMMHKLPSIVGSSIKVNRLIELPAEPLKLPRRKNFKASDNLSSDVNQNQGDGDFVEIPVPTAHLGPGLPVSVRLLSARRRSGMLGEGRYRGWHKPIPPSPSILFHCHGGGFVAQSSKSHELYLRDWAVALDCPILSVDYSLAPEAPFPRALQEVYYAYCWLLNNTELLGTTAERVVCAGDSAGANLSIGVALKCIEQGVRVPDGLFLAYCPTLVSFVPSPARLLCLMDPLLPFGFMMRCLRAYAAPAQETLQQNAKQVEDAAQIRNVPKSNVGSLNSSRRTSMARSPLSPLEASMNPDDESSDTFASASASYHSQTVERTDLPHTEGDNSSCVSFEDDSQPIVHYPIEISADPPKDTASAAYIDNFLDKYLIDTATMEVTEETAPEAVQAQANGHAKISSDDDILVETGRDLVAIDTLQGRLQEAVNNITNTLTRCTQSYEIHGSNVMAQQDVRNMDALIARSPSEEFAFDVPKDPFLSPYWASDEWLSQLPETKILTLNMDPCLDDCVMFAKKLKRLGRQVDLEILEGLPHGFLNFTMLSNEAMEGSKKCIKSLQTLLQTDSKTKNIDKANSMDEEESSSPSASLAAS

Target ORF information:

RefSeq Version	NM_137619.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Hormone-sensitive lipase (Hsl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137619.3

ORF Insert Sequence:

ATGATTGACG CGGCTTCCGC CGAGCGGGCA AGCCCCCAGT TTATTGCCCT CTTCAATGAC 
AATCTGAAAC TGGGCCACGA GGACGACGGC CCACAGGCAG TCAATGGAAA GGACACCCAC 
CAGCACGTAC CCGCCGACCT GGAGGCGACC TATGGAACCT TGTACGCCGC CTGCCAGGAT 
CACGCCGCCT TCTTTGCCCG GGACCACACC GAATTCGGCC AGCGCCTGCA TGCCGCCCAC 
ATTGCCTGGC AGGACTTCAT TGTCTTGGCC AACCGACTGG TCCAGCAGAT CGATGCCTTC 
GCCCACGAGT ACGATTTTGA CGAGCAGACG CCGGGCAATG GATACAGGAG CTTCATCTAC 
GTGACCAACG CCTGCATCGC GCATGGCATT AGCATCTGCC AGCAGCTGAC AGCCACCCGT 
TCCACCATAT TCTTCCGCAA GAAGTTCTAC ATGAAGGAGG TTGAGGCCTG TTCCCAGCTG 
TTGTCGTCGC TGTGCACATG TCTGCAGTAC CTGCTGATCC TTCGCCAGTG GTCGGCTAGC 
ACGGGTGATC TGTTCGCATG CGGAAATCAC ACTGCCGAGC AGCTCTTCGA GCTGGGCGAC 
ACCATCAACC AGTATTGCTT TTACGGACGC TGCCTGGGCT TTCAGTATGG CGACTCGATA 
CGCGGAGTTC TCCGATTCCT CGGCATCAGC ATGGCCAGCT ACTCGGAGTC GTACTATTCG 
CAAGAGGGCG ACGGCCCCAT CGTTAAGACC ACACGCAGCC TGTGGACCAG CGGCAAGTAT 
CTTATGAATC CGGAACTGCG CGCCCGTCGC ATCGTTAACA TCTCGCAGAA TGCTAAGATA 
GACTTTTGCA AATCCTTTTG GTTTCTGGCC GAGTCCGAGA TGATGCACAA GCTGCCCAGC 
ATTGTGGGCA GCTCCATTAA GGTAAACCGG CTCATCGAAT TGCCAGCCGA GCCGCTAAAG 
CTGCCTCGCC GAAAGAATTT CAAGGCATCG GACAATCTCA GCAGTGACGT TAACCAAAAC 
CAAGGCGATG GTGATTTTGT GGAGATTCCC GTGCCCACGG CACATTTGGG ACCTGGTTTA 
CCGGTCTCTG TTCGCTTGCT GAGCGCGAGA CGACGTTCGG GAATGCTGGG CGAGGGTCGC 
TATCGCGGCT GGCACAAGCC CATTCCGCCA AGTCCTTCGA TTCTGTTTCA CTGCCATGGC 
GGTGGCTTTG TGGCCCAATC GTCCAAGTCC CACGAACTGT ATTTGCGCGA CTGGGCCGTG 
GCACTGGACT GTCCTATTCT CTCGGTGGAT TACAGCCTAG CTCCTGAGGC GCCATTTCCA 
CGCGCCCTGC AAGAAGTTTA CTACGCTTAC TGCTGGCTTC TGAACAATAC CGAACTCCTA 
GGCACAACAG CTGAGCGAGT TGTATGCGCA GGTGACTCGG CTGGGGCAAA TCTGTCAATT 
GGAGTAGCCC TCAAGTGCAT TGAGCAGGGA GTTCGAGTGC CAGATGGTCT GTTTCTGGCC 
TACTGCCCAA CTCTCGTCAG CTTTGTGCCC AGTCCGGCCC GCCTTCTCTG TTTGATGGAT 
CCCCTGCTGC CTTTCGGCTT CATGATGCGC TGCCTTCGTG CCTATGCCGC TCCCGCCCAG 
GAGACACTTC AGCAGAATGC CAAGCAAGTC GAGGATGCCG CCCAGATACG CAATGTCCCC 
AAGTCGAACG TGGGAAGCTT GAACTCCAGT CGCCGCACCT CGATGGCCAG AAGTCCGCTT 
TCGCCGCTGG AAGCCAGCAT GAACCCAGAT GACGAAAGCA GCGACACCTT TGCCAGTGCC 
TCAGCCTCTT ACCACAGCCA GACGGTGGAG CGTACCGATC TCCCCCACAC CGAAGGCGAC 
AACAGCTCGT GCGTCTCGTT CGAGGATGAT TCACAGCCCA TTGTGCACTA CCCAATCGAA 
ATATCAGCCG ACCCACCTAA GGATACCGCC TCTGCAGCCT ATATTGACAA TTTTCTGGAC 
AAGTATCTCA TTGACACGGC CACAATGGAG GTAACCGAAG AGACAGCTCC CGAAGCGGTG 
CAAGCGCAGG CAAATGGACA TGCGAAGATC AGTTCCGATG ATGATATTCT AGTGGAGACT 
GGTCGGGATC TTGTTGCAAT AGATACGCTG CAAGGCCGCC TGCAGGAAGC CGTCAACAAC 
ATAACCAACA CCTTGACGCG CTGCACGCAG TCCTACGAGA TTCACGGAAG CAACGTGATG 
GCTCAACAGG ATGTGCGCAA CATGGACGCA CTGATAGCAC GGAGTCCCAG CGAGGAGTTT 
GCATTCGATG TGCCTAAGGA TCCATTCCTG TCGCCGTACT GGGCCAGTGA TGAGTGGCTT 
TCCCAACTGC CGGAGACCAA AATACTTACA CTTAACATGG ACCCCTGTCT GGATGATTGC 
GTTATGTTTG CCAAGAAACT GAAGCGGCTT GGGCGACAAG TTGACTTGGA AATACTTGAG 
GGGTTGCCGC ACGGATTCCT AAATTTCACA ATGTTATCCA ACGAAGCCAT GGAGGGCTCA 
AAGAAGTGCA TCAAATCTCT ACAGACGTTG CTTCAAACCG ATTCGAAGAC CAAGAACATT 
GACAAAGCCA ACTCGATGGA CGAGGAGGAG TCCTCCAGTC CCAGTGCAAG TCTAGCCGCT 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsl ( NM_166381.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725941.1 Drosophila melanogaster Hormone-sensitive lipase (Hsl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13116
Clone ID Related Accession (Same CDS sequence)	NM_137619.3 , NM_166381.2 , NM_166382.2
Accession Version	NM_166381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Hormone-sensitive lipase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166381.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGACG CGGCTTCCGC CGAGCGGGCA AGCCCCCAGT TTATTGCCCT CTTCAATGAC 
AATCTGAAAC TGGGCCACGA GGACGACGGC CCACAGGCAG TCAATGGAAA GGACACCCAC 
CAGCACGTAC CCGCCGACCT GGAGGCGACC TATGGAACCT TGTACGCCGC CTGCCAGGAT 
CACGCCGCCT TCTTTGCCCG GGACCACACC GAATTCGGCC AGCGCCTGCA TGCCGCCCAC 
ATTGCCTGGC AGGACTTCAT TGTCTTGGCC AACCGACTGG TCCAGCAGAT CGATGCCTTC 
GCCCACGAGT ACGATTTTGA CGAGCAGACG CCGGGCAATG GATACAGGAG CTTCATCTAC 
GTGACCAACG CCTGCATCGC GCATGGCATT AGCATCTGCC AGCAGCTGAC AGCCACCCGT 
TCCACCATAT TCTTCCGCAA GAAGTTCTAC ATGAAGGAGG TTGAGGCCTG TTCCCAGCTG 
TTGTCGTCGC TGTGCACATG TCTGCAGTAC CTGCTGATCC TTCGCCAGTG GTCGGCTAGC 
ACGGGTGATC TGTTCGCATG CGGAAATCAC ACTGCCGAGC AGCTCTTCGA GCTGGGCGAC 
ACCATCAACC AGTATTGCTT TTACGGACGC TGCCTGGGCT TTCAGTATGG CGACTCGATA 
CGCGGAGTTC TCCGATTCCT CGGCATCAGC ATGGCCAGCT ACTCGGAGTC GTACTATTCG 
CAAGAGGGCG ACGGCCCCAT CGTTAAGACC ACACGCAGCC TGTGGACCAG CGGCAAGTAT 
CTTATGAATC CGGAACTGCG CGCCCGTCGC ATCGTTAACA TCTCGCAGAA TGCTAAGATA 
GACTTTTGCA AATCCTTTTG GTTTCTGGCC GAGTCCGAGA TGATGCACAA GCTGCCCAGC 
ATTGTGGGCA GCTCCATTAA GGTAAACCGG CTCATCGAAT TGCCAGCCGA GCCGCTAAAG 
CTGCCTCGCC GAAAGAATTT CAAGGCATCG GACAATCTCA GCAGTGACGT TAACCAAAAC 
CAAGGCGATG GTGATTTTGT GGAGATTCCC GTGCCCACGG CACATTTGGG ACCTGGTTTA 
CCGGTCTCTG TTCGCTTGCT GAGCGCGAGA CGACGTTCGG GAATGCTGGG CGAGGGTCGC 
TATCGCGGCT GGCACAAGCC CATTCCGCCA AGTCCTTCGA TTCTGTTTCA CTGCCATGGC 
GGTGGCTTTG TGGCCCAATC GTCCAAGTCC CACGAACTGT ATTTGCGCGA CTGGGCCGTG 
GCACTGGACT GTCCTATTCT CTCGGTGGAT TACAGCCTAG CTCCTGAGGC GCCATTTCCA 
CGCGCCCTGC AAGAAGTTTA CTACGCTTAC TGCTGGCTTC TGAACAATAC CGAACTCCTA 
GGCACAACAG CTGAGCGAGT TGTATGCGCA GGTGACTCGG CTGGGGCAAA TCTGTCAATT 
GGAGTAGCCC TCAAGTGCAT TGAGCAGGGA GTTCGAGTGC CAGATGGTCT GTTTCTGGCC 
TACTGCCCAA CTCTCGTCAG CTTTGTGCCC AGTCCGGCCC GCCTTCTCTG TTTGATGGAT 
CCCCTGCTGC CTTTCGGCTT CATGATGCGC TGCCTTCGTG CCTATGCCGC TCCCGCCCAG 
GAGACACTTC AGCAGAATGC CAAGCAAGTC GAGGATGCCG CCCAGATACG CAATGTCCCC 
AAGTCGAACG TGGGAAGCTT GAACTCCAGT CGCCGCACCT CGATGGCCAG AAGTCCGCTT 
TCGCCGCTGG AAGCCAGCAT GAACCCAGAT GACGAAAGCA GCGACACCTT TGCCAGTGCC 
TCAGCCTCTT ACCACAGCCA GACGGTGGAG CGTACCGATC TCCCCCACAC CGAAGGCGAC 
AACAGCTCGT GCGTCTCGTT CGAGGATGAT TCACAGCCCA TTGTGCACTA CCCAATCGAA 
ATATCAGCCG ACCCACCTAA GGATACCGCC TCTGCAGCCT ATATTGACAA TTTTCTGGAC 
AAGTATCTCA TTGACACGGC CACAATGGAG GTAACCGAAG AGACAGCTCC CGAAGCGGTG 
CAAGCGCAGG CAAATGGACA TGCGAAGATC AGTTCCGATG ATGATATTCT AGTGGAGACT 
GGTCGGGATC TTGTTGCAAT AGATACGCTG CAAGGCCGCC TGCAGGAAGC CGTCAACAAC 
ATAACCAACA CCTTGACGCG CTGCACGCAG TCCTACGAGA TTCACGGAAG CAACGTGATG 
GCTCAACAGG ATGTGCGCAA CATGGACGCA CTGATAGCAC GGAGTCCCAG CGAGGAGTTT 
GCATTCGATG TGCCTAAGGA TCCATTCCTG TCGCCGTACT GGGCCAGTGA TGAGTGGCTT 
TCCCAACTGC CGGAGACCAA AATACTTACA CTTAACATGG ACCCCTGTCT GGATGATTGC 
GTTATGTTTG CCAAGAAACT GAAGCGGCTT GGGCGACAAG TTGACTTGGA AATACTTGAG 
GGGTTGCCGC ACGGATTCCT AAATTTCACA ATGTTATCCA ACGAAGCCAT GGAGGGCTCA 
AAGAAGTGCA TCAAATCTCT ACAGACGTTG CTTCAAACCG ATTCGAAGAC CAAGAACATT 
GACAAAGCCA ACTCGATGGA CGAGGAGGAG TCCTCCAGTC CCAGTGCAAG TCTAGCCGCT 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725941.1
CDS	142..2787
Translation	MIDAASAERASPQFIALFNDNLKLGHEDDGPQAVNGKDTHQHVPADLEATYGTLYAACQDHAAFFARDHTEFGQRLHAAHIAWQDFIVLANRLVQQIDAFAHEYDFDEQTPGNGYRSFIYVTNACIAHGISICQQLTATRSTIFFRKKFYMKEVEACSQLLSSLCTCLQYLLILRQWSASTGDLFACGNHTAEQLFELGDTINQYCFYGRCLGFQYGDSIRGVLRFLGISMASYSESYYSQEGDGPIVKTTRSLWTSGKYLMNPELRARRIVNISQNAKIDFCKSFWFLAESEMMHKLPSIVGSSIKVNRLIELPAEPLKLPRRKNFKASDNLSSDVNQNQGDGDFVEIPVPTAHLGPGLPVSVRLLSARRRSGMLGEGRYRGWHKPIPPSPSILFHCHGGGFVAQSSKSHELYLRDWAVALDCPILSVDYSLAPEAPFPRALQEVYYAYCWLLNNTELLGTTAERVVCAGDSAGANLSIGVALKCIEQGVRVPDGLFLAYCPTLVSFVPSPARLLCLMDPLLPFGFMMRCLRAYAAPAQETLQQNAKQVEDAAQIRNVPKSNVGSLNSSRRTSMARSPLSPLEASMNPDDESSDTFASASASYHSQTVERTDLPHTEGDNSSCVSFEDDSQPIVHYPIEISADPPKDTASAAYIDNFLDKYLIDTATMEVTEETAPEAVQAQANGHAKISSDDDILVETGRDLVAIDTLQGRLQEAVNNITNTLTRCTQSYEIHGSNVMAQQDVRNMDALIARSPSEEFAFDVPKDPFLSPYWASDEWLSQLPETKILTLNMDPCLDDCVMFAKKLKRLGRQVDLEILEGLPHGFLNFTMLSNEAMEGSKKCIKSLQTLLQTDSKTKNIDKANSMDEEESSSPSASLAAS

Target ORF information:

RefSeq Version	NM_166381.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Hormone-sensitive lipase (Hsl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166381.2

ORF Insert Sequence:

ATGATTGACG CGGCTTCCGC CGAGCGGGCA AGCCCCCAGT TTATTGCCCT CTTCAATGAC 
AATCTGAAAC TGGGCCACGA GGACGACGGC CCACAGGCAG TCAATGGAAA GGACACCCAC 
CAGCACGTAC CCGCCGACCT GGAGGCGACC TATGGAACCT TGTACGCCGC CTGCCAGGAT 
CACGCCGCCT TCTTTGCCCG GGACCACACC GAATTCGGCC AGCGCCTGCA TGCCGCCCAC 
ATTGCCTGGC AGGACTTCAT TGTCTTGGCC AACCGACTGG TCCAGCAGAT CGATGCCTTC 
GCCCACGAGT ACGATTTTGA CGAGCAGACG CCGGGCAATG GATACAGGAG CTTCATCTAC 
GTGACCAACG CCTGCATCGC GCATGGCATT AGCATCTGCC AGCAGCTGAC AGCCACCCGT 
TCCACCATAT TCTTCCGCAA GAAGTTCTAC ATGAAGGAGG TTGAGGCCTG TTCCCAGCTG 
TTGTCGTCGC TGTGCACATG TCTGCAGTAC CTGCTGATCC TTCGCCAGTG GTCGGCTAGC 
ACGGGTGATC TGTTCGCATG CGGAAATCAC ACTGCCGAGC AGCTCTTCGA GCTGGGCGAC 
ACCATCAACC AGTATTGCTT TTACGGACGC TGCCTGGGCT TTCAGTATGG CGACTCGATA 
CGCGGAGTTC TCCGATTCCT CGGCATCAGC ATGGCCAGCT ACTCGGAGTC GTACTATTCG 
CAAGAGGGCG ACGGCCCCAT CGTTAAGACC ACACGCAGCC TGTGGACCAG CGGCAAGTAT 
CTTATGAATC CGGAACTGCG CGCCCGTCGC ATCGTTAACA TCTCGCAGAA TGCTAAGATA 
GACTTTTGCA AATCCTTTTG GTTTCTGGCC GAGTCCGAGA TGATGCACAA GCTGCCCAGC 
ATTGTGGGCA GCTCCATTAA GGTAAACCGG CTCATCGAAT TGCCAGCCGA GCCGCTAAAG 
CTGCCTCGCC GAAAGAATTT CAAGGCATCG GACAATCTCA GCAGTGACGT TAACCAAAAC 
CAAGGCGATG GTGATTTTGT GGAGATTCCC GTGCCCACGG CACATTTGGG ACCTGGTTTA 
CCGGTCTCTG TTCGCTTGCT GAGCGCGAGA CGACGTTCGG GAATGCTGGG CGAGGGTCGC 
TATCGCGGCT GGCACAAGCC CATTCCGCCA AGTCCTTCGA TTCTGTTTCA CTGCCATGGC 
GGTGGCTTTG TGGCCCAATC GTCCAAGTCC CACGAACTGT ATTTGCGCGA CTGGGCCGTG 
GCACTGGACT GTCCTATTCT CTCGGTGGAT TACAGCCTAG CTCCTGAGGC GCCATTTCCA 
CGCGCCCTGC AAGAAGTTTA CTACGCTTAC TGCTGGCTTC TGAACAATAC CGAACTCCTA 
GGCACAACAG CTGAGCGAGT TGTATGCGCA GGTGACTCGG CTGGGGCAAA TCTGTCAATT 
GGAGTAGCCC TCAAGTGCAT TGAGCAGGGA GTTCGAGTGC CAGATGGTCT GTTTCTGGCC 
TACTGCCCAA CTCTCGTCAG CTTTGTGCCC AGTCCGGCCC GCCTTCTCTG TTTGATGGAT 
CCCCTGCTGC CTTTCGGCTT CATGATGCGC TGCCTTCGTG CCTATGCCGC TCCCGCCCAG 
GAGACACTTC AGCAGAATGC CAAGCAAGTC GAGGATGCCG CCCAGATACG CAATGTCCCC 
AAGTCGAACG TGGGAAGCTT GAACTCCAGT CGCCGCACCT CGATGGCCAG AAGTCCGCTT 
TCGCCGCTGG AAGCCAGCAT GAACCCAGAT GACGAAAGCA GCGACACCTT TGCCAGTGCC 
TCAGCCTCTT ACCACAGCCA GACGGTGGAG CGTACCGATC TCCCCCACAC CGAAGGCGAC 
AACAGCTCGT GCGTCTCGTT CGAGGATGAT TCACAGCCCA TTGTGCACTA CCCAATCGAA 
ATATCAGCCG ACCCACCTAA GGATACCGCC TCTGCAGCCT ATATTGACAA TTTTCTGGAC 
AAGTATCTCA TTGACACGGC CACAATGGAG GTAACCGAAG AGACAGCTCC CGAAGCGGTG 
CAAGCGCAGG CAAATGGACA TGCGAAGATC AGTTCCGATG ATGATATTCT AGTGGAGACT 
GGTCGGGATC TTGTTGCAAT AGATACGCTG CAAGGCCGCC TGCAGGAAGC CGTCAACAAC 
ATAACCAACA CCTTGACGCG CTGCACGCAG TCCTACGAGA TTCACGGAAG CAACGTGATG 
GCTCAACAGG ATGTGCGCAA CATGGACGCA CTGATAGCAC GGAGTCCCAG CGAGGAGTTT 
GCATTCGATG TGCCTAAGGA TCCATTCCTG TCGCCGTACT GGGCCAGTGA TGAGTGGCTT 
TCCCAACTGC CGGAGACCAA AATACTTACA CTTAACATGG ACCCCTGTCT GGATGATTGC 
GTTATGTTTG CCAAGAAACT GAAGCGGCTT GGGCGACAAG TTGACTTGGA AATACTTGAG 
GGGTTGCCGC ACGGATTCCT AAATTTCACA ATGTTATCCA ACGAAGCCAT GGAGGGCTCA 
AAGAAGTGCA TCAAATCTCT ACAGACGTTG CTTCAAACCG ATTCGAAGAC CAAGAACATT 
GACAAAGCCA ACTCGATGGA CGAGGAGGAG TCCTCCAGTC CCAGTGCAAG TCTAGCCGCT 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsl ( NM_166382.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725942.1 Drosophila melanogaster Hormone-sensitive lipase (Hsl), transcript variant C, mRNA.

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CloneID	OFa13116
Clone ID Related Accession (Same CDS sequence)	NM_137619.3 , NM_166381.2 , NM_166382.2
Accession Version	NM_166382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Hormone-sensitive lipase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166382.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGACG CGGCTTCCGC CGAGCGGGCA AGCCCCCAGT TTATTGCCCT CTTCAATGAC 
AATCTGAAAC TGGGCCACGA GGACGACGGC CCACAGGCAG TCAATGGAAA GGACACCCAC 
CAGCACGTAC CCGCCGACCT GGAGGCGACC TATGGAACCT TGTACGCCGC CTGCCAGGAT 
CACGCCGCCT TCTTTGCCCG GGACCACACC GAATTCGGCC AGCGCCTGCA TGCCGCCCAC 
ATTGCCTGGC AGGACTTCAT TGTCTTGGCC AACCGACTGG TCCAGCAGAT CGATGCCTTC 
GCCCACGAGT ACGATTTTGA CGAGCAGACG CCGGGCAATG GATACAGGAG CTTCATCTAC 
GTGACCAACG CCTGCATCGC GCATGGCATT AGCATCTGCC AGCAGCTGAC AGCCACCCGT 
TCCACCATAT TCTTCCGCAA GAAGTTCTAC ATGAAGGAGG TTGAGGCCTG TTCCCAGCTG 
TTGTCGTCGC TGTGCACATG TCTGCAGTAC CTGCTGATCC TTCGCCAGTG GTCGGCTAGC 
ACGGGTGATC TGTTCGCATG CGGAAATCAC ACTGCCGAGC AGCTCTTCGA GCTGGGCGAC 
ACCATCAACC AGTATTGCTT TTACGGACGC TGCCTGGGCT TTCAGTATGG CGACTCGATA 
CGCGGAGTTC TCCGATTCCT CGGCATCAGC ATGGCCAGCT ACTCGGAGTC GTACTATTCG 
CAAGAGGGCG ACGGCCCCAT CGTTAAGACC ACACGCAGCC TGTGGACCAG CGGCAAGTAT 
CTTATGAATC CGGAACTGCG CGCCCGTCGC ATCGTTAACA TCTCGCAGAA TGCTAAGATA 
GACTTTTGCA AATCCTTTTG GTTTCTGGCC GAGTCCGAGA TGATGCACAA GCTGCCCAGC 
ATTGTGGGCA GCTCCATTAA GGTAAACCGG CTCATCGAAT TGCCAGCCGA GCCGCTAAAG 
CTGCCTCGCC GAAAGAATTT CAAGGCATCG GACAATCTCA GCAGTGACGT TAACCAAAAC 
CAAGGCGATG GTGATTTTGT GGAGATTCCC GTGCCCACGG CACATTTGGG ACCTGGTTTA 
CCGGTCTCTG TTCGCTTGCT GAGCGCGAGA CGACGTTCGG GAATGCTGGG CGAGGGTCGC 
TATCGCGGCT GGCACAAGCC CATTCCGCCA AGTCCTTCGA TTCTGTTTCA CTGCCATGGC 
GGTGGCTTTG TGGCCCAATC GTCCAAGTCC CACGAACTGT ATTTGCGCGA CTGGGCCGTG 
GCACTGGACT GTCCTATTCT CTCGGTGGAT TACAGCCTAG CTCCTGAGGC GCCATTTCCA 
CGCGCCCTGC AAGAAGTTTA CTACGCTTAC TGCTGGCTTC TGAACAATAC CGAACTCCTA 
GGCACAACAG CTGAGCGAGT TGTATGCGCA GGTGACTCGG CTGGGGCAAA TCTGTCAATT 
GGAGTAGCCC TCAAGTGCAT TGAGCAGGGA GTTCGAGTGC CAGATGGTCT GTTTCTGGCC 
TACTGCCCAA CTCTCGTCAG CTTTGTGCCC AGTCCGGCCC GCCTTCTCTG TTTGATGGAT 
CCCCTGCTGC CTTTCGGCTT CATGATGCGC TGCCTTCGTG CCTATGCCGC TCCCGCCCAG 
GAGACACTTC AGCAGAATGC CAAGCAAGTC GAGGATGCCG CCCAGATACG CAATGTCCCC 
AAGTCGAACG TGGGAAGCTT GAACTCCAGT CGCCGCACCT CGATGGCCAG AAGTCCGCTT 
TCGCCGCTGG AAGCCAGCAT GAACCCAGAT GACGAAAGCA GCGACACCTT TGCCAGTGCC 
TCAGCCTCTT ACCACAGCCA GACGGTGGAG CGTACCGATC TCCCCCACAC CGAAGGCGAC 
AACAGCTCGT GCGTCTCGTT CGAGGATGAT TCACAGCCCA TTGTGCACTA CCCAATCGAA 
ATATCAGCCG ACCCACCTAA GGATACCGCC TCTGCAGCCT ATATTGACAA TTTTCTGGAC 
AAGTATCTCA TTGACACGGC CACAATGGAG GTAACCGAAG AGACAGCTCC CGAAGCGGTG 
CAAGCGCAGG CAAATGGACA TGCGAAGATC AGTTCCGATG ATGATATTCT AGTGGAGACT 
GGTCGGGATC TTGTTGCAAT AGATACGCTG CAAGGCCGCC TGCAGGAAGC CGTCAACAAC 
ATAACCAACA CCTTGACGCG CTGCACGCAG TCCTACGAGA TTCACGGAAG CAACGTGATG 
GCTCAACAGG ATGTGCGCAA CATGGACGCA CTGATAGCAC GGAGTCCCAG CGAGGAGTTT 
GCATTCGATG TGCCTAAGGA TCCATTCCTG TCGCCGTACT GGGCCAGTGA TGAGTGGCTT 
TCCCAACTGC CGGAGACCAA AATACTTACA CTTAACATGG ACCCCTGTCT GGATGATTGC 
GTTATGTTTG CCAAGAAACT GAAGCGGCTT GGGCGACAAG TTGACTTGGA AATACTTGAG 
GGGTTGCCGC ACGGATTCCT AAATTTCACA ATGTTATCCA ACGAAGCCAT GGAGGGCTCA 
AAGAAGTGCA TCAAATCTCT ACAGACGTTG CTTCAAACCG ATTCGAAGAC CAAGAACATT 
GACAAAGCCA ACTCGATGGA CGAGGAGGAG TCCTCCAGTC CCAGTGCAAG TCTAGCCGCT 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725942.1
CDS	201..2846
Translation	MIDAASAERASPQFIALFNDNLKLGHEDDGPQAVNGKDTHQHVPADLEATYGTLYAACQDHAAFFARDHTEFGQRLHAAHIAWQDFIVLANRLVQQIDAFAHEYDFDEQTPGNGYRSFIYVTNACIAHGISICQQLTATRSTIFFRKKFYMKEVEACSQLLSSLCTCLQYLLILRQWSASTGDLFACGNHTAEQLFELGDTINQYCFYGRCLGFQYGDSIRGVLRFLGISMASYSESYYSQEGDGPIVKTTRSLWTSGKYLMNPELRARRIVNISQNAKIDFCKSFWFLAESEMMHKLPSIVGSSIKVNRLIELPAEPLKLPRRKNFKASDNLSSDVNQNQGDGDFVEIPVPTAHLGPGLPVSVRLLSARRRSGMLGEGRYRGWHKPIPPSPSILFHCHGGGFVAQSSKSHELYLRDWAVALDCPILSVDYSLAPEAPFPRALQEVYYAYCWLLNNTELLGTTAERVVCAGDSAGANLSIGVALKCIEQGVRVPDGLFLAYCPTLVSFVPSPARLLCLMDPLLPFGFMMRCLRAYAAPAQETLQQNAKQVEDAAQIRNVPKSNVGSLNSSRRTSMARSPLSPLEASMNPDDESSDTFASASASYHSQTVERTDLPHTEGDNSSCVSFEDDSQPIVHYPIEISADPPKDTASAAYIDNFLDKYLIDTATMEVTEETAPEAVQAQANGHAKISSDDDILVETGRDLVAIDTLQGRLQEAVNNITNTLTRCTQSYEIHGSNVMAQQDVRNMDALIARSPSEEFAFDVPKDPFLSPYWASDEWLSQLPETKILTLNMDPCLDDCVMFAKKLKRLGRQVDLEILEGLPHGFLNFTMLSNEAMEGSKKCIKSLQTLLQTDSKTKNIDKANSMDEEESSSPSASLAAS

Target ORF information:

RefSeq Version	NM_166382.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Hormone-sensitive lipase (Hsl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166382.2

ORF Insert Sequence:

ATGATTGACG CGGCTTCCGC CGAGCGGGCA AGCCCCCAGT TTATTGCCCT CTTCAATGAC 
AATCTGAAAC TGGGCCACGA GGACGACGGC CCACAGGCAG TCAATGGAAA GGACACCCAC 
CAGCACGTAC CCGCCGACCT GGAGGCGACC TATGGAACCT TGTACGCCGC CTGCCAGGAT 
CACGCCGCCT TCTTTGCCCG GGACCACACC GAATTCGGCC AGCGCCTGCA TGCCGCCCAC 
ATTGCCTGGC AGGACTTCAT TGTCTTGGCC AACCGACTGG TCCAGCAGAT CGATGCCTTC 
GCCCACGAGT ACGATTTTGA CGAGCAGACG CCGGGCAATG GATACAGGAG CTTCATCTAC 
GTGACCAACG CCTGCATCGC GCATGGCATT AGCATCTGCC AGCAGCTGAC AGCCACCCGT 
TCCACCATAT TCTTCCGCAA GAAGTTCTAC ATGAAGGAGG TTGAGGCCTG TTCCCAGCTG 
TTGTCGTCGC TGTGCACATG TCTGCAGTAC CTGCTGATCC TTCGCCAGTG GTCGGCTAGC 
ACGGGTGATC TGTTCGCATG CGGAAATCAC ACTGCCGAGC AGCTCTTCGA GCTGGGCGAC 
ACCATCAACC AGTATTGCTT TTACGGACGC TGCCTGGGCT TTCAGTATGG CGACTCGATA 
CGCGGAGTTC TCCGATTCCT CGGCATCAGC ATGGCCAGCT ACTCGGAGTC GTACTATTCG 
CAAGAGGGCG ACGGCCCCAT CGTTAAGACC ACACGCAGCC TGTGGACCAG CGGCAAGTAT 
CTTATGAATC CGGAACTGCG CGCCCGTCGC ATCGTTAACA TCTCGCAGAA TGCTAAGATA 
GACTTTTGCA AATCCTTTTG GTTTCTGGCC GAGTCCGAGA TGATGCACAA GCTGCCCAGC 
ATTGTGGGCA GCTCCATTAA GGTAAACCGG CTCATCGAAT TGCCAGCCGA GCCGCTAAAG 
CTGCCTCGCC GAAAGAATTT CAAGGCATCG GACAATCTCA GCAGTGACGT TAACCAAAAC 
CAAGGCGATG GTGATTTTGT GGAGATTCCC GTGCCCACGG CACATTTGGG ACCTGGTTTA 
CCGGTCTCTG TTCGCTTGCT GAGCGCGAGA CGACGTTCGG GAATGCTGGG CGAGGGTCGC 
TATCGCGGCT GGCACAAGCC CATTCCGCCA AGTCCTTCGA TTCTGTTTCA CTGCCATGGC 
GGTGGCTTTG TGGCCCAATC GTCCAAGTCC CACGAACTGT ATTTGCGCGA CTGGGCCGTG 
GCACTGGACT GTCCTATTCT CTCGGTGGAT TACAGCCTAG CTCCTGAGGC GCCATTTCCA 
CGCGCCCTGC AAGAAGTTTA CTACGCTTAC TGCTGGCTTC TGAACAATAC CGAACTCCTA 
GGCACAACAG CTGAGCGAGT TGTATGCGCA GGTGACTCGG CTGGGGCAAA TCTGTCAATT 
GGAGTAGCCC TCAAGTGCAT TGAGCAGGGA GTTCGAGTGC CAGATGGTCT GTTTCTGGCC 
TACTGCCCAA CTCTCGTCAG CTTTGTGCCC AGTCCGGCCC GCCTTCTCTG TTTGATGGAT 
CCCCTGCTGC CTTTCGGCTT CATGATGCGC TGCCTTCGTG CCTATGCCGC TCCCGCCCAG 
GAGACACTTC AGCAGAATGC CAAGCAAGTC GAGGATGCCG CCCAGATACG CAATGTCCCC 
AAGTCGAACG TGGGAAGCTT GAACTCCAGT CGCCGCACCT CGATGGCCAG AAGTCCGCTT 
TCGCCGCTGG AAGCCAGCAT GAACCCAGAT GACGAAAGCA GCGACACCTT TGCCAGTGCC 
TCAGCCTCTT ACCACAGCCA GACGGTGGAG CGTACCGATC TCCCCCACAC CGAAGGCGAC 
AACAGCTCGT GCGTCTCGTT CGAGGATGAT TCACAGCCCA TTGTGCACTA CCCAATCGAA 
ATATCAGCCG ACCCACCTAA GGATACCGCC TCTGCAGCCT ATATTGACAA TTTTCTGGAC 
AAGTATCTCA TTGACACGGC CACAATGGAG GTAACCGAAG AGACAGCTCC CGAAGCGGTG 
CAAGCGCAGG CAAATGGACA TGCGAAGATC AGTTCCGATG ATGATATTCT AGTGGAGACT 
GGTCGGGATC TTGTTGCAAT AGATACGCTG CAAGGCCGCC TGCAGGAAGC CGTCAACAAC 
ATAACCAACA CCTTGACGCG CTGCACGCAG TCCTACGAGA TTCACGGAAG CAACGTGATG 
GCTCAACAGG ATGTGCGCAA CATGGACGCA CTGATAGCAC GGAGTCCCAG CGAGGAGTTT 
GCATTCGATG TGCCTAAGGA TCCATTCCTG TCGCCGTACT GGGCCAGTGA TGAGTGGCTT 
TCCCAACTGC CGGAGACCAA AATACTTACA CTTAACATGG ACCCCTGTCT GGATGATTGC 
GTTATGTTTG CCAAGAAACT GAAGCGGCTT GGGCGACAAG TTGACTTGGA AATACTTGAG 
GGGTTGCCGC ACGGATTCCT AAATTTCACA ATGTTATCCA ACGAAGCCAT GGAGGGCTCA 
AAGAAGTGCA TCAAATCTCT ACAGACGTTG CTTCAAACCG ATTCGAAGAC CAAGAACATT 
GACAAAGCCA ACTCGATGGA CGAGGAGGAG TCCTCCAGTC CCAGTGCAAG TCTAGCCGCT 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hsl cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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