wcd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wcd
Entrez Gene ID	36831
Full Name	wicked
Synonyms	CG7989,Dmel\CG7989,Wcd,l(2)k07824,l(2)kO7824
General protein information	Preferred Names wicked Names CG7989 gene product from transcript CG7989-RA CG7989-PA lethal (2) k07824 wcd-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53C1-53C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_137277.4	NP_611121.1	wicked

Pathways from BioSystems

Homologies

Homo sapiens (human)	UTP18	NP_057085.2
Bos taurus (cattle)	UTP18	NP_001092349.1
Gallus gallus (chicken)	UTP18	XP_003642362.2
	UTP18	XP_001096494.1
Rattus norvegicus (Norway rat)	Utp18	NP_001128511.1
Arabidopsis thaliana (thale cress)	AT5G14050	NP_196909.1
Canis lupus familiaris (dog)	UTP18	XP_005624739.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009046	XP_319798.2
Oryza sativa (rice)	Os07g0603200	NP_001060211.1
Pan troglodytes (chimpanzee)	UTP18	XP_001159780.2
Mus musculus (house mouse)	Utp18	NP_001013393.1
Danio rerio (zebrafish)	utp18	NP_001070232.1
Drosophila melanogaster (fruit fly)	wcd	NP_611121.1
Xenopus tropicalis (tropical clawed frog)	utp18	NP_001016635.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results describe mutations in a Drosophila melanogaster gene, wicked (wcd, CG7989), which induce premature differentiation of germline stem cells (GSCs), and show that Wcd is a member of the U3 snoRNP complex required for pre-ribosomal RNA maturation.
Title: Live-imaging of single stem cells within their niche reveals that a U3snoRNP component segregates asymmetrically and is required for self-renewal in Drosophila.

The following wcd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wcd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10217	NM_137277.4 Latest version!	Drosophila melanogaster wicked (wcd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wcd ( NM_137277.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611121.1 Drosophila melanogaster wicked (wcd), mRNA.

Price & Availability↑

CloneID	OFa10217
Clone ID Related Accession (Same CDS sequence)	NM_137277.4
Accession Version	NM_137277.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wicked
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137277.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTCAG ACGAGTCCAG CGACGGTTTG GAGGAGCTGC AGAGTCTGAA GGCCCTCTAC 
GGCCAGCAGG AGCAAGAGAA ACCGGCAAAG ATCAAAAGGG AACGTTATAT TCCAAAGGCC 
TCGCAGGCCA AGGAGCTCAA CTATGTGGAG GTGCCCATGG AAAAGGTGCT CTTCGGCGAC 
AGGCAGCGAC TGCTCACCAA TCTAGCCAAG TCCGTGGGAC AAAAGTTGCC GAACGATGAC 
GAAGACGAGC AGGAGGAAAA TCCCGGCCAG GCAAAGCCAG GCGACAAGCG GAAAGCCGCC 
TGGTCGGATT CCGATGACGA GGACCTGCAG GTGGGCGATG TCAAGAAGGC CACAAAGCAC 
ACGGGTCCAC TGAACCACCT GCGCAAGGAT AAATCGTACA AGGAGTATCT TACGGCGCGA 
TTCCAGCGCA CGCTTAACCA ACCCAAGTGG GCGGAAAAGA AGGTCAAAAA TGAGGACGAC 
GAGGACGTAT CCTCCGACGA AGAACTGCTG CGGACGGTTG GCTTTATCGA TCGTAAAGCC 
CGGAACAGTG ATCTGCCCCA GAAGACTCTG AATTTTAAAC GGGTCAAAGA CCTGAACAGG 
GCCACATATG CCGAGGGTAA TGCCACCAGC ATTCAGTTTC ATCCAACCAG TACTGCTGCT 
CTAGTGGCGG GCATGAATGG CCTAGCCACC ATCTACGCCG TGGATGGGCA AAAGAACGAA 
AGGTTGCACA ACATGCGCTT CAAGAAGTTC CCGCTGGCGT GCTCCAGAAT TGCTCCATGC 
GGCACAAGGG CGTTCTTTGG TTCTGTGAAA CCATTCTACT ACTCCTACGA CTTGCTGGAA 
GCCAAGGAAT CGAAGCTAAA GCTGCCGGGC GCAATGGAAT TTATGCACCG CTTTGAGGTG 
TCTCCATGTG GCAAATTTAT CGTCACTGCT GGCAAGTTTG GAGCCATTCA CCTGCTCACG 
GCCAAAACAA ATGAGCTGCT GCACAGCTTC AAGCAGGAGG GCAAAGTGAA GGGATTCACA 
TGGTCTAGTG ACTCCAAGAG GATCCTCGTC TGCGGTTCCA CTTCCAATGT CAGCGTGCTG 
AACCTGCGCC AGAACCTCAT CGAGCACATC TTCATGGACG ACGGCTGTAT ACATGGAGAG 
TCCATTCAAC TGTCTCCCAA CCAACGCCTA CTGGCCACCG GCAGTCAAGA GGGCGTGGTC 
AACATCTACG ACTACGAGAG CATTTTCGCA TCCAAGGCCC CACAACCCGA GAAGCGCTTC 
ATGAACCTAC GGACTGCCAT CACGGATCTC CAGTTCAATC ACTCATCTGA GCTACTGGCG 
ATGTGCTCCA GTGAGGCGCC GAATGCTTTT AAGCTGGCCC ATTTCCCCAG CGCCACTGTG 
TACTCCAATT TCCCGGCACA GAACGAGAAG GTTGGCTTTG TGACCTCCAT GGCCTTTTCG 
CCGCACAGCA GCTTCCTGGC CTTTGCCACT AAGGGCAAAC AGGTGCCGCT CTTTAGGCTT 
AAGTACTTCA AGGGTTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611121.1
CDS	106..1626
Translation	MSSDESSDGLEELQSLKALYGQQEQEKPAKIKRERYIPKASQAKELNYVEVPMEKVLFGDRQRLLTNLAKSVGQKLPNDDEDEQEENPGQAKPGDKRKAAWSDSDDEDLQVGDVKKATKHTGPLNHLRKDKSYKEYLTARFQRTLNQPKWAEKKVKNEDDEDVSSDEELLRTVGFIDRKARNSDLPQKTLNFKRVKDLNRATYAEGNATSIQFHPTSTAALVAGMNGLATIYAVDGQKNERLHNMRFKKFPLACSRIAPCGTRAFFGSVKPFYYSYDLLEAKESKLKLPGAMEFMHRFEVSPCGKFIVTAGKFGAIHLLTAKTNELLHSFKQEGKVKGFTWSSDSKRILVCGSTSNVSVLNLRQNLIEHIFMDDGCIHGESIQLSPNQRLLATGSQEGVVNIYDYESIFASKAPQPEKRFMNLRTAITDLQFNHSSELLAMCSSEAPNAFKLAHFPSATVYSNFPAQNEKVGFVTSMAFSPHSSFLAFATKGKQVPLFRLKYFKGY

Target ORF information:

RefSeq Version	NM_137277.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wicked (wcd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137277.4

ORF Insert Sequence:

ATGAGTTCAG ACGAGTCCAG CGACGGTTTG GAGGAGCTGC AGAGTCTGAA GGCCCTCTAC 
GGCCAGCAGG AGCAAGAGAA ACCGGCAAAG ATCAAAAGGG AACGTTATAT TCCAAAGGCC 
TCGCAGGCCA AGGAGCTCAA CTATGTGGAG GTGCCCATGG AAAAGGTGCT CTTCGGCGAC 
AGGCAGCGAC TGCTCACCAA TCTAGCCAAG TCCGTGGGAC AAAAGTTGCC GAACGATGAC 
GAAGACGAGC AGGAGGAAAA TCCCGGCCAG GCAAAGCCAG GCGACAAGCG GAAAGCCGCC 
TGGTCGGATT CCGATGACGA GGACCTGCAG GTGGGCGATG TCAAGAAGGC CACAAAGCAC 
ACGGGTCCAC TGAACCACCT GCGCAAGGAT AAATCGTACA AGGAGTATCT TACGGCGCGA 
TTCCAGCGCA CGCTTAACCA ACCCAAGTGG GCGGAAAAGA AGGTCAAAAA TGAGGACGAC 
GAGGACGTAT CCTCCGACGA AGAACTGCTG CGGACGGTTG GCTTTATCGA TCGTAAAGCC 
CGGAACAGTG ATCTGCCCCA GAAGACTCTG AATTTTAAAC GGGTCAAAGA CCTGAACAGG 
GCCACATATG CCGAGGGTAA TGCCACCAGC ATTCAGTTTC ATCCAACCAG TACTGCTGCT 
CTAGTGGCGG GCATGAATGG CCTAGCCACC ATCTACGCCG TGGATGGGCA AAAGAACGAA 
AGGTTGCACA ACATGCGCTT CAAGAAGTTC CCGCTGGCGT GCTCCAGAAT TGCTCCATGC 
GGCACAAGGG CGTTCTTTGG TTCTGTGAAA CCATTCTACT ACTCCTACGA CTTGCTGGAA 
GCCAAGGAAT CGAAGCTAAA GCTGCCGGGC GCAATGGAAT TTATGCACCG CTTTGAGGTG 
TCTCCATGTG GCAAATTTAT CGTCACTGCT GGCAAGTTTG GAGCCATTCA CCTGCTCACG 
GCCAAAACAA ATGAGCTGCT GCACAGCTTC AAGCAGGAGG GCAAAGTGAA GGGATTCACA 
TGGTCTAGTG ACTCCAAGAG GATCCTCGTC TGCGGTTCCA CTTCCAATGT CAGCGTGCTG 
AACCTGCGCC AGAACCTCAT CGAGCACATC TTCATGGACG ACGGCTGTAT ACATGGAGAG 
TCCATTCAAC TGTCTCCCAA CCAACGCCTA CTGGCCACCG GCAGTCAAGA GGGCGTGGTC 
AACATCTACG ACTACGAGAG CATTTTCGCA TCCAAGGCCC CACAACCCGA GAAGCGCTTC 
ATGAACCTAC GGACTGCCAT CACGGATCTC CAGTTCAATC ACTCATCTGA GCTACTGGCG 
ATGTGCTCCA GTGAGGCGCC GAATGCTTTT AAGCTGGCCC ATTTCCCCAG CGCCACTGTG 
TACTCCAATT TCCCGGCACA GAACGAGAAG GTTGGCTTTG TGACCTCCAT GGCCTTTTCG 
CCGCACAGCA GCTTCCTGGC CTTTGCCACT AAGGGCAAAC AGGTGCCGCT CTTTAGGCTT 
AAGTACTTCA AGGGTTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

wcd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報