Cdk5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdk5
Entrez Gene ID	36727
Full Name	Cyclin-dependent kinase 5
Synonyms	CDK5,CG8203,DmCdk5,Dmel\CG8203,cdk5
General protein information	Preferred Names Cyclin-dependent kinase 5 Names CG8203 gene product from transcript CG8203-RA CG8203-PA Cdk5-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52A13-52A13

mRNA and Protein(s)

mRNA	Protein	Name
NM_057732.4	NP_477080.1	Cyclin-dependent kinase 5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cdk5	NP_543161.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005772	XP_315787.4
Saccharomyces cerevisiae (baker's yeast)	PHO85	NP_015294.1
Neurospora crassa	NCU07580	XP_958274.1
Xenopus tropicalis (tropical clawed frog)	cdk5	NP_001072776.1
Homo sapiens (human)	CDK5	NP_004926.1
	CDK5	XP_001102523.1
Canis lupus familiaris (dog)	CDK5	XP_005629809.1
Bos taurus (cattle)	CDK5	NP_776442.1
Gallus gallus (chicken)	CDK5	NP_001129258.1
Eremothecium gossypii	AGOS_AGL242C	NP_986425.2
Pan troglodytes (chimpanzee)	CDK5	XP_519523.2
Mus musculus (house mouse)	Cdk5	NP_031694.1
Danio rerio (zebrafish)	cdk5	NP_571794.1
Drosophila melanogaster (fruit fly)	Cdk5	NP_477080.1
Caenorhabditis elegans (roundworm)	cdk-5	NP_499783.1
Kluyveromyces lactis	KLLA0D11990g	XP_453596.1
Schizosaccharomyces pombe (fission yeast)	pef1	NP_587921.1
Magnaporthe oryzae (rice blast fungus)	MGG_04660	XP_003713655.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data show that Cdk5 regulates the onset and extent of remodeling of the Drosophila mushroom body.
Title: Cdk5 regulates developmental remodeling of mushroom body neurons in Drosophila.

The CDK5 phosphorylates MEKK1, and together, they activate the JNK pathway for apoptosis.
Title: CDK5 and MEKK1 mediate pro-apoptotic signalling following endoplasmic reticulum stress in an autosomal dominant retinitis pigmentosa model.

The data of this study demonstrated that Cdk5/p35 kinase is a key regulator of the development and maintenance of the axon initial segment in Drosophila.
Title: Cdk5 regulates the size of an axon initial segment-like compartment in mushroom body neurons of the Drosophila central brain.

In Drosophila the cdk5 is needed for locomotive behavior and NMJ elaboration.
Title: Drosophila cdk5 is needed for locomotive behavior and NMJ elaboration, but seems dispensable for synaptic transmission.

Cdk5/p35 did not have major effects on tau toxicity or phosphorylation.
Title: Dissociation of tau toxicity and phosphorylation: role of GSK-3beta, MARK and Cdk5 in a Drosophila model.

The following Cdk5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdk5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10109	NM_057732.4 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 5 (Cdk5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdk5 ( NM_057732.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477080.1 Drosophila melanogaster Cyclin-dependent kinase 5 (Cdk5), mRNA.

Price & Availability↑

CloneID	OFa10109
Clone ID Related Accession (Same CDS sequence)	NM_057732.4
Accession Version	NM_057732.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057732.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAAGT ACGATAAAAT GGAGAAGATC GGGGAGGGCA CCTACGGCAC GGTGTTCAAG 
GGTCGCAACC GCGACACCAT GGAAATAGTG GCCCTGAAAC GGGTGCGACT GGACGAGGAC 
GACGAGGGTG TGCCCAGTTC TGCCCTCCGG GAGATCTGCC TGCTGAAGGA GCTGAAGCAC 
AAGAACATTG TGCGCCTCAT AGACGTCCTG CACTCGGACA AGAAACTCAC CCTGGTCTTC 
GAGCACTGCG ACCAGGACCT CAAGAAGTAC TTTGACAGCC TCAACGGGGA GATCGACATG 
GCCGTCTGCA GGAGCTTTAT GCTCCAACTG CTCCGCGGAC TGGCATTCTG CCACAGCCAT 
AATGTCCTGC ATCGCGATCT GAAACCACAG AACCTGCTGA TCAACAAAAA CGGCGAGCTA 
AAGCTAGCTG ACTTTGGTCT CGCTAGAGCC TTTGGCATTC CCGTGAAGTG CTACTCCGCA 
GAAGTGGTGA CCCTGTGGTA CCGACCACCC GATGTCCTAT TTGGAGCCAA GCTCTATACG 
ACCAGCATAG ACATGTGGTC CGCTGGATGC ATTTTGGCCG AACTGGCGGA CGCTGGACGA 
CCGCTGTTTC CTGGCTCTGA CGTGCTCGAT CAGCTGATGA AGATCTTCCG TGTGCTGGGC 
ACCCCCAACG AGGACTCCTG GCCAGGAGTT TCGCACCTCT CCGACTACGT GGCGCTTCCA 
TCTTTTCCGG CCATCACCTC GTGGAGTCAG CTGGTACCTA GGCTAAACTC CAAGGGACGC 
GACCTGCTGC AAAAGCTGCT CATCTGCCGG CCAAATCAGC GAATTAGCGC GGAGGCGGCG 
ATGCAGCATC CCTACTTTAC GGACAGCAGC TCTTCGGGAC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477080.1
CDS	116..1000
Translation	MQKYDKMEKIGEGTYGTVFKGRNRDTMEIVALKRVRLDEDDEGVPSSALREICLLKELKHKNIVRLIDVLHSDKKLTLVFEHCDQDLKKYFDSLNGEIDMAVCRSFMLQLLRGLAFCHSHNVLHRDLKPQNLLINKNGELKLADFGLARAFGIPVKCYSAEVVTLWYRPPDVLFGAKLYTTSIDMWSAGCILAELADAGRPLFPGSDVLDQLMKIFRVLGTPNEDSWPGVSHLSDYVALPSFPAITSWSQLVPRLNSKGRDLLQKLLICRPNQRISAEAAMQHPYFTDSSSSGH

Target ORF information:

RefSeq Version	NM_057732.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 5 (Cdk5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057732.4

ORF Insert Sequence:

ATGCAGAAGT ACGATAAAAT GGAGAAGATC GGGGAGGGCA CCTACGGCAC GGTGTTCAAG 
GGTCGCAACC GCGACACCAT GGAAATAGTG GCCCTGAAAC GGGTGCGACT GGACGAGGAC 
GACGAGGGTG TGCCCAGTTC TGCCCTCCGG GAGATCTGCC TGCTGAAGGA GCTGAAGCAC 
AAGAACATTG TGCGCCTCAT AGACGTCCTG CACTCGGACA AGAAACTCAC CCTGGTCTTC 
GAGCACTGCG ACCAGGACCT CAAGAAGTAC TTTGACAGCC TCAACGGGGA GATCGACATG 
GCCGTCTGCA GGAGCTTTAT GCTCCAACTG CTCCGCGGAC TGGCATTCTG CCACAGCCAT 
AATGTCCTGC ATCGCGATCT GAAACCACAG AACCTGCTGA TCAACAAAAA CGGCGAGCTA 
AAGCTAGCTG ACTTTGGTCT CGCTAGAGCC TTTGGCATTC CCGTGAAGTG CTACTCCGCA 
GAAGTGGTGA CCCTGTGGTA CCGACCACCC GATGTCCTAT TTGGAGCCAA GCTCTATACG 
ACCAGCATAG ACATGTGGTC CGCTGGATGC ATTTTGGCCG AACTGGCGGA CGCTGGACGA 
CCGCTGTTTC CTGGCTCTGA CGTGCTCGAT CAGCTGATGA AGATCTTCCG TGTGCTGGGC 
ACCCCCAACG AGGACTCCTG GCCAGGAGTT TCGCACCTCT CCGACTACGT GGCGCTTCCA 
TCTTTTCCGG CCATCACCTC GTGGAGTCAG CTGGTACCTA GGCTAAACTC CAAGGGACGC 
GACCTGCTGC AAAAGCTGCT CATCTGCCGG CCAAATCAGC GAATTAGCGC GGAGGCGGCG 
ATGCAGCATC CCTACTTTAC GGACAGCAGC TCTTCGGGAC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cdk5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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