Mt2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mt2
Entrez Gene ID	34632
Full Name	Methyltransferase 2
Synonyms	CG10692,DNMT,DNMT2,DmMT2,Dmel\CG10692,Dnmt,Dnmt2,dDNMT,dDNMT2,dDnmt2,dnmt2,nmt2
General protein information	Preferred Names Methyltransferase 2 Names CG10692 gene product from transcript CG10692-RC CG10692-PC CG10692-PD DNA methyltransferase 2 DNA(5-cytosine) methyltransferase Mt2-PC Mt2-PD cytosine-5 methyltransferase methyltransferase 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 33C4-33C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_058127.4	NP_477475.2	methyltransferase 2, isoform D
NM_001042890.2	NP_001036355.1	methyltransferase 2, isoform C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRDMT1	XP_848593.1
Pan troglodytes (chimpanzee)	TRDMT1	XP_001151840.2
Rattus norvegicus (Norway rat)	Trdmt1	NP_001026813.1
Gallus gallus (chicken)	TRDMT1	NP_001020002.1
Bos taurus (cattle)	TRDMT1	NP_861528.1
Mus musculus (house mouse)	Trdmt1	NP_034197.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004101	XP_312975.5
Homo sapiens (human)	TRDMT1	NP_004403.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012836	XP_306830.2
Arabidopsis thaliana (thale cress)	DNMT2	NP_568474.1
Xenopus tropicalis (tropical clawed frog)	trdmt1	NP_001004959.2
Danio rerio (zebrafish)	trdmt1	NP_001018153.1
Drosophila melanogaster (fruit fly)	Mt2	NP_001036355.1
Schizosaccharomyces pombe (fission yeast)	pmt1	NP_595687.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wolbachia elevates D. melanogaster Mt2 expression to block Sindbis virus infection.
Title: Wolbachia elevates host methyltransferase expression to block an RNA virus early during infection.

The differences found in the electrostatic potentials may be linked with different affinities and processivity of DNMT2 for its different substrates (DNA, RNA or tRNA) and even for interactions with other proteins involved in the epigenetic mechanisms
Title: Linking epigenetic function to electrostatics: The DNMT2 structural model example.

Mt2, the fly's only candidate DNA methyltransferase, is not necessary for the observed DNA methylation.
Title: Now you see it: genome methylation makes a comeback in Drosophila.

No difference was detected in recombination rates between Dnmt2 -/- and Dnmt2 +/- flies in two euchromatic regions and one heterochromatic region across the genome.
Title: No detectable effect of the DNA methyltransferase DNMT2 on Drosophila meiotic recombination.

Though the genome of Drosophila is essentially unmethylated, it contains a 5-methylcytosine oxidase.
Title: The mysterious presence of a 5-methylcytosine oxidase in the Drosophila genome: possible explanations.

The following Mt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14509	NM_058127.4 Latest version!	Drosophila melanogaster methyltransferase 2, transcript variant D (Mt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14509	NM_001042890.2 Latest version!	Drosophila melanogaster methyltransferase 2, transcript variant C (Mt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mt2 ( NM_058127.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477475.2 Drosophila melanogaster methyltransferase 2, transcript variant D (Mt2), mRNA.

Price & Availability↑

CloneID	OFa14509
Clone ID Related Accession (Same CDS sequence)	NM_058127.4 , NM_001042890.2
Accession Version	NM_058127.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	methyltransferase 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_058127.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTATTTC GGGTCTTAGA ACTATTTAGT GGCATTGGCG GCATGCATTA TGCCTTTAAT 
TATGCCCAAT TGGATGGACA AATAGTTGCC GCCTTGGATG TCAACACCGT GGCCAATGCG 
GTTTATGCGC ACAATTACGG CAGTAATTTG GTGAAAACTA GGAATATTCA AAGCCTGAGT 
GTAAAGGAAG TCACAAAGCT TCAGGCCAAC ATGCTGCTGA TGTCCCCGCC ATGTCAGCCC 
CACACTCGCC AGGGATTGCA AAGGGACACG GAAGACAAGC GATCGGATGC ACTTACTCAT 
CTGTGTGGAC TGATCCCAGA GTGCCAGGAA CTGGAGTACA TACTCATGGA AAACGTCAAG 
GGTTTCGAGA GCTCACAGGC GCGAAATCAG TTTATTGAAT CGCTGGAGCG GTCGGGATTC 
CATTGGCGGG AGTTTATTCT AACGCCAACG CAATTCAATG TGCCAAATAC TCGATATCGC 
TACTATTGCA TCGCCCGCAA GGGTGCAGAC TTTCCATTTG CCGGTGGAAA GATCTGGGAA 
GAAATGCCGG GTGCTATAGC CCAGAATCAG GGTCTTTCAC AAATTGCCGA GATTGTGGAG 
GAAAATGTAT CACCCGATTT CCTGGTGCCC GACGATGTCT TGACCAAAAG AGTGCTGGTC 
ATGGACATAA TACATCCTGC TCAAAGTAGA TCCATGTGCT TTACAAAGGG CTACACCCAT 
TACACCGAGG GCACGGGCTC TGCATACACA CCGCTTTCGG AGGACGAATC CCACCGCATC 
TTCGAGTTGG TCAAGGAAAT TGACACAAGT AATCAGGATG CATCGAAGTC GGAGAAGATT 
TTGCAGCAAC GCTTGGACCT GTTGCACCAG GTGAGACTGC GCTATTTCAC GCCACGGGAA 
GTTGCTCGTC TAATGAGTTT TCCGGAGAAT TTTGAATTTC CGCCAGAAAC AACGAATCGA 
CAAAAGTATC GACTGCTGGG AAATAGTATT AATGTAAAGG TTGTCGGTGA ACTTATTAAA 
TTGCTGACGA TAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477475.2
CDS	37..1074
Translation	MVFRVLELFSGIGGMHYAFNYAQLDGQIVAALDVNTVANAVYAHNYGSNLVKTRNIQSLSVKEVTKLQANMLLMSPPCQPHTRQGLQRDTEDKRSDALTHLCGLIPECQELEYILMENVKGFESSQARNQFIESLERSGFHWREFILTPTQFNVPNTRYRYYCIARKGADFPFAGGKIWEEMPGAIAQNQGLSQIAEIVEENVSPDFLVPDDVLTKRVLVMDIIHPAQSRSMCFTKGYTHYTEGTGSAYTPLSEDESHRIFELVKEIDTSNQDASKSEKILQQRLDLLHQVRLRYFTPREVARLMSFPENFEFPPETTNRQKYRLLGNSINVKVVGELIKLLTIK

Target ORF information:

RefSeq Version	NM_058127.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster methyltransferase 2, transcript variant D (Mt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058127.4

ORF Insert Sequence:

ATGGTATTTC GGGTCTTAGA ACTATTTAGT GGCATTGGCG GCATGCATTA TGCCTTTAAT 
TATGCCCAAT TGGATGGACA AATAGTTGCC GCCTTGGATG TCAACACCGT GGCCAATGCG 
GTTTATGCGC ACAATTACGG CAGTAATTTG GTGAAAACTA GGAATATTCA AAGCCTGAGT 
GTAAAGGAAG TCACAAAGCT TCAGGCCAAC ATGCTGCTGA TGTCCCCGCC ATGTCAGCCC 
CACACTCGCC AGGGATTGCA AAGGGACACG GAAGACAAGC GATCGGATGC ACTTACTCAT 
CTGTGTGGAC TGATCCCAGA GTGCCAGGAA CTGGAGTACA TACTCATGGA AAACGTCAAG 
GGTTTCGAGA GCTCACAGGC GCGAAATCAG TTTATTGAAT CGCTGGAGCG GTCGGGATTC 
CATTGGCGGG AGTTTATTCT AACGCCAACG CAATTCAATG TGCCAAATAC TCGATATCGC 
TACTATTGCA TCGCCCGCAA GGGTGCAGAC TTTCCATTTG CCGGTGGAAA GATCTGGGAA 
GAAATGCCGG GTGCTATAGC CCAGAATCAG GGTCTTTCAC AAATTGCCGA GATTGTGGAG 
GAAAATGTAT CACCCGATTT CCTGGTGCCC GACGATGTCT TGACCAAAAG AGTGCTGGTC 
ATGGACATAA TACATCCTGC TCAAAGTAGA TCCATGTGCT TTACAAAGGG CTACACCCAT 
TACACCGAGG GCACGGGCTC TGCATACACA CCGCTTTCGG AGGACGAATC CCACCGCATC 
TTCGAGTTGG TCAAGGAAAT TGACACAAGT AATCAGGATG CATCGAAGTC GGAGAAGATT 
TTGCAGCAAC GCTTGGACCT GTTGCACCAG GTGAGACTGC GCTATTTCAC GCCACGGGAA 
GTTGCTCGTC TAATGAGTTT TCCGGAGAAT TTTGAATTTC CGCCAGAAAC AACGAATCGA 
CAAAAGTATC GACTGCTGGG AAATAGTATT AATGTAAAGG TTGTCGGTGA ACTTATTAAA 
TTGCTGACGA TAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mt2 ( NM_001042890.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036355.1 Drosophila melanogaster methyltransferase 2, transcript variant C (Mt2), mRNA.

Price & Availability↑

CloneID	OFa14509
Clone ID Related Accession (Same CDS sequence)	NM_058127.4 , NM_001042890.2
Accession Version	NM_001042890.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	methyltransferase 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001042890.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTATTTC GGGTCTTAGA ACTATTTAGT GGCATTGGCG GCATGCATTA TGCCTTTAAT 
TATGCCCAAT TGGATGGACA AATAGTTGCC GCCTTGGATG TCAACACCGT GGCCAATGCG 
GTTTATGCGC ACAATTACGG CAGTAATTTG GTGAAAACTA GGAATATTCA AAGCCTGAGT 
GTAAAGGAAG TCACAAAGCT TCAGGCCAAC ATGCTGCTGA TGTCCCCGCC ATGTCAGCCC 
CACACTCGCC AGGGATTGCA AAGGGACACG GAAGACAAGC GATCGGATGC ACTTACTCAT 
CTGTGTGGAC TGATCCCAGA GTGCCAGGAA CTGGAGTACA TACTCATGGA AAACGTCAAG 
GGTTTCGAGA GCTCACAGGC GCGAAATCAG TTTATTGAAT CGCTGGAGCG GTCGGGATTC 
CATTGGCGGG AGTTTATTCT AACGCCAACG CAATTCAATG TGCCAAATAC TCGATATCGC 
TACTATTGCA TCGCCCGCAA GGGTGCAGAC TTTCCATTTG CCGGTGGAAA GATCTGGGAA 
GAAATGCCGG GTGCTATAGC CCAGAATCAG GGTCTTTCAC AAATTGCCGA GATTGTGGAG 
GAAAATGTAT CACCCGATTT CCTGGTGCCC GACGATGTCT TGACCAAAAG AGTGCTGGTC 
ATGGACATAA TACATCCTGC TCAAAGTAGA TCCATGTGCT TTACAAAGGG CTACACCCAT 
TACACCGAGG GCACGGGCTC TGCATACACA CCGCTTTCGG AGGACGAATC CCACCGCATC 
TTCGAGTTGG TCAAGGAAAT TGACACAAGT AATCAGGATG CATCGAAGTC GGAGAAGATT 
TTGCAGCAAC GCTTGGACCT GTTGCACCAG GTGAGACTGC GCTATTTCAC GCCACGGGAA 
GTTGCTCGTC TAATGAGTTT TCCGGAGAAT TTTGAATTTC CGCCAGAAAC AACGAATCGA 
CAAAAGTATC GACTGCTGGG AAATAGTATT AATGTAAAGG TTGTCGGTGA ACTTATTAAA 
TTGCTGACGA TAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036355.1
CDS	62..1099
Translation	MVFRVLELFSGIGGMHYAFNYAQLDGQIVAALDVNTVANAVYAHNYGSNLVKTRNIQSLSVKEVTKLQANMLLMSPPCQPHTRQGLQRDTEDKRSDALTHLCGLIPECQELEYILMENVKGFESSQARNQFIESLERSGFHWREFILTPTQFNVPNTRYRYYCIARKGADFPFAGGKIWEEMPGAIAQNQGLSQIAEIVEENVSPDFLVPDDVLTKRVLVMDIIHPAQSRSMCFTKGYTHYTEGTGSAYTPLSEDESHRIFELVKEIDTSNQDASKSEKILQQRLDLLHQVRLRYFTPREVARLMSFPENFEFPPETTNRQKYRLLGNSINVKVVGELIKLLTIK

Target ORF information:

RefSeq Version	NM_001042890.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster methyltransferase 2, transcript variant C (Mt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042890.2

ORF Insert Sequence:

ATGGTATTTC GGGTCTTAGA ACTATTTAGT GGCATTGGCG GCATGCATTA TGCCTTTAAT 
TATGCCCAAT TGGATGGACA AATAGTTGCC GCCTTGGATG TCAACACCGT GGCCAATGCG 
GTTTATGCGC ACAATTACGG CAGTAATTTG GTGAAAACTA GGAATATTCA AAGCCTGAGT 
GTAAAGGAAG TCACAAAGCT TCAGGCCAAC ATGCTGCTGA TGTCCCCGCC ATGTCAGCCC 
CACACTCGCC AGGGATTGCA AAGGGACACG GAAGACAAGC GATCGGATGC ACTTACTCAT 
CTGTGTGGAC TGATCCCAGA GTGCCAGGAA CTGGAGTACA TACTCATGGA AAACGTCAAG 
GGTTTCGAGA GCTCACAGGC GCGAAATCAG TTTATTGAAT CGCTGGAGCG GTCGGGATTC 
CATTGGCGGG AGTTTATTCT AACGCCAACG CAATTCAATG TGCCAAATAC TCGATATCGC 
TACTATTGCA TCGCCCGCAA GGGTGCAGAC TTTCCATTTG CCGGTGGAAA GATCTGGGAA 
GAAATGCCGG GTGCTATAGC CCAGAATCAG GGTCTTTCAC AAATTGCCGA GATTGTGGAG 
GAAAATGTAT CACCCGATTT CCTGGTGCCC GACGATGTCT TGACCAAAAG AGTGCTGGTC 
ATGGACATAA TACATCCTGC TCAAAGTAGA TCCATGTGCT TTACAAAGGG CTACACCCAT 
TACACCGAGG GCACGGGCTC TGCATACACA CCGCTTTCGG AGGACGAATC CCACCGCATC 
TTCGAGTTGG TCAAGGAAAT TGACACAAGT AATCAGGATG CATCGAAGTC GGAGAAGATT 
TTGCAGCAAC GCTTGGACCT GTTGCACCAG GTGAGACTGC GCTATTTCAC GCCACGGGAA 
GTTGCTCGTC TAATGAGTTT TCCGGAGAAT TTTGAATTTC CGCCAGAAAC AACGAATCGA 
CAAAAGTATC GACTGCTGGG AAATAGTATT AATGTAAAGG TTGTCGGTGA ACTTATTAAA 
TTGCTGACGA TAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mt2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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