lid cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	lid
Entrez Gene ID	33837
Full Name	little imaginal discs
Synonyms	CG9088,Dmel\CG9088,KDM5,Kdm5,LID,Lid,dJARID1,dKDM5/LID,l(2)10424
General protein information	Preferred Names little imaginal discs Names CG9088 gene product from transcript CG9088-RF CG9088-PA CG9088-PB CG9088-PC CG9088-PD CG9088-PE CG9088-PF lid-PA lid-PB lid-PC lid-PD lid-PE lid-PF little imaginal disks
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26B2-26B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_078762.6	NP_523486.1	little imaginal discs, isoform A
NM_001298720.1	NP_001285649.1	little imaginal discs, isoform F
NM_001258980.1	NP_001245909.1	little imaginal discs, isoform D
NM_001258979.2	NP_001245908.1	little imaginal discs, isoform C
NM_164671.1	NP_723140.1	little imaginal discs, isoform B
NM_001258981.2	NP_001245910.1	little imaginal discs, isoform E

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KDM5A	XP_005637389.1
Mus musculus (house mouse)	Kdm5a	NP_666109.2
Xenopus tropicalis (tropical clawed frog)	kdm5a	XP_002941946.2
Homo sapiens (human)	KDM5A	NP_001036068.1
Pan troglodytes (chimpanzee)	KDM5A	XP_003313474.1
Rattus norvegicus (Norway rat)	Kdm5a	NP_001264106.1
Gallus gallus (chicken)	KDM5A	XP_004937981.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004854	XP_314337.4
	KDM5A	XP_002798486.1
Drosophila melanogaster (fruit fly)	lid	NP_001245909.1
Bos taurus (cattle)	KDM5A	NP_001192554.1
Danio rerio (zebrafish)	kdm5a	XP_003201688.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Therefore Kdm5/Lid controls chromatin architecture in meiotic prophase I oocytes independently of its demethylase activity.
Title: Kdm5/Lid Regulates Chromosome Architecture in Meiotic Prophase I Independently of Its Histone Demethylase Activity.

we demonstrate a direct, critical role for KDM5 in the activation of genes required for the integrity and functioning of mitochondria
Title: The Histone Demethylase KDM5 Activates Gene Expression by Recognizing Chromatin Context through Its PHD Reader Motif.

dKDM5/LID regulates Drosophila hematopoietic development
Title: The Drosophila histone demethylase dKDM5/LID regulates hematopoietic development.

KDM5 interacts with Foxo to modulate cellular levels of oxidative stress.KDM5 interacts with the lysine deacetylase HDAC4 to promote Foxo deacetylation, which affects Foxo DNA binding.
Title: KDM5 interacts with Foxo to modulate cellular levels of oxidative stress.

The results indicate that, at developmental genes, dKDM5/LID and ASH2 coordinately regulate H3K4me3 at the transcription-start site and that this dynamic regulation contributes to transcription.
Title: dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes.

The following lid gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lid cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13853	NM_001298720.1 Latest version!	Drosophila melanogaster little imaginal discs (lid), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa13853	NM_164671.1 Latest version!	Drosophila melanogaster little imaginal discs (lid), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa13853	NM_001258981.2 Latest version!	Drosophila melanogaster little imaginal discs (lid), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa13853	NM_001258980.1 Latest version!	Drosophila melanogaster little imaginal discs (lid), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa13853	NM_001258979.2 Latest version!	Drosophila melanogaster little imaginal discs (lid), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa13853	NM_078762.6 Latest version!	Drosophila melanogaster little imaginal discs (lid), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lid ( NM_078762.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523486.1 Drosophila melanogaster little imaginal discs (lid), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13853
Clone ID Related Accession (Same CDS sequence)	NM_078762.6 , NM_001298720.1 , NM_001258980.1 , NM_001258979.2 , NM_164671.1 , NM_001258981.2
Accession Version	NM_078762.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	little imaginal discs, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078762.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523486.1
CDS	246..5762
Translation	MSAKTEADNTTAANSGGGGVGSGTSSGGGASANGTATPARRLRTRNSTGNGTNSGSESVKKSNANDEPSTPVTPAGATGSHTHAPPGISPAVMERPMPSVPMNHASSSVSASKKYHNSCPHPTPTPAPTGHKKSVHTQPHSSNKFDQGKNEEFHFDTPPECPVFRPTTEEFKNPLAYISKIRSIAEKCGIAKILPPATWSPPFAVDVDKLRFVPRVQRLNELEAKTRVKLNFLDQIAKFWELQGSSLKIPMVERKALDLYTLHRIVQEEGGMEQTTKDRKWAKVANRMQYPSSKSVGATLKAHYERILHPFEVYTSGKVLGPTPTSSGSGSTPVKLEDGGGTDYKAHEIPTRQQIAPPNETNTRRSKRFGNSNASCGLSGVTPTTKPSAGVFVKTETKEEFKRDLLSSFNAVNSGGSPLATGTTANTRGASQKKGGEPPALIVDPLMKYICHICNRGDVEESMLLCDGCDDSYHTFCLLPPLTSIPKGEWLCPRCVVEEVSKPQEAFGFEQAEREYTLQQFGQMADQFKQEYFRKPVHLVPTEMVEREFWRIVSSIDEDVTVEYGADLHTMDHGSGFPTKSSLYLLPGDQEYAESSWNLNNLPLLEDSILGHINADISGMNAPWMYVGMCFAAFCWHNEDHWSYSINYLHWGEPKTWYGVPGSCAEQFEETMKQAAPELFSSQPDLLHQLVTIMNPNILMNNRVPVFRTDQHAGEFVITFPRAYHAGFNQGYNFAEAVNFAPADWLKMGRECVNHYSMLRRFCVFSHDELVCKMALEPAKLTFGIATACYIDMAEMVDTEKKLRKSLLEWGVTRAERRAFELVNDDERHCQECNTTCFLSAVACECNDKLIVCLRHYTVLCGCAPEKHTLIYRYTLDEMPLMLQKLKVKAHSFERWLSRCRDIVDAHTPTSVTLQELQELCKEAETKKFPSSLLIDRLNAAAVEAEKCVTVIQQLGINKVRTRSDHNQEAAQYKLTMEELELFVQEIDNLCCIIDEGASVRELLVLGKQFVERSESQLQLSLESLEESELETLINEGSSLRIELQQLDLLQKRLKQCKWYKRSQGLRETSSKLTYQDVKNLLHIAAADLDPTDPYVDKEMRKLQQIGADIEAWESQAAKYFRRLTQQHELGEIEQFLKSASDINGQVPSHGLLKDALRKAREWLRAVEQLQQNNHVTYCHTLEAMIERGLNIPIQLEELSRMQGHLNSAHQWKDNTACAFLKKGTFYTLLEVLMPRSDAINIDSDLKPRFQDDFLKEKNPAEIVASFKHAEEQELLDMRELRRQNMNKNPMRDMFCLCKSEFRNLMFNCQLCRDWFHEDCVPPPSATNQNGIVNGGSGPGTNRPKWLCPSCVRSKRPRLETILPLLVQLQQLPIRLPEDEALRCLAERAMNWQDRARKALSSPDVSAAQEAIMAQQQQKRRSEGGAGVGNISSPRKPRRRGSLTKEASGSTESDADDDDDEDECRLRIVEDNFSNDEDEPRTAPATSTVNSDLLKLLSDSEIENLLDLMMEGDLLEVSLDETQELWRILETMPPTLLQAEAMERVVQYMQRQRQQHTNPLPTSGAEDSNDSLMVQNSPNSNSNSGGATGSASNSGRNKKRRSNDTGGNSAVPRKKQSTPKQTPGKKGSAAAARKSDAKASPAASTTPGADADAENKQANGGNTNSSTGSGGGNSATTTPTPGSTHKKRKRTSTTATNNNNNNNNNSTNNSNSSTNLNSNTTSGQGAATGGNNATGGQKKHAQRSQQAAQEDDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQIKPDDDYICIRCTKTVAIGTQGSGHSMSVASTTTPGKQRAVQSAR

Target ORF information:

RefSeq Version	NM_078762.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster little imaginal discs (lid), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078762.6

ORF Insert Sequence:

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lid ( NM_001298720.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285649.1 Drosophila melanogaster little imaginal discs (lid), transcript variant F, mRNA.

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CloneID	OFa13853
Clone ID Related Accession (Same CDS sequence)	NM_078762.6 , NM_001298720.1 , NM_001258980.1 , NM_001258979.2 , NM_164671.1 , NM_001258981.2
Accession Version	NM_001298720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	little imaginal discs, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285649.1
CDS	246..5762
Translation	MSAKTEADNTTAANSGGGGVGSGTSSGGGASANGTATPARRLRTRNSTGNGTNSGSESVKKSNANDEPSTPVTPAGATGSHTHAPPGISPAVMERPMPSVPMNHASSSVSASKKYHNSCPHPTPTPAPTGHKKSVHTQPHSSNKFDQGKNEEFHFDTPPECPVFRPTTEEFKNPLAYISKIRSIAEKCGIAKILPPATWSPPFAVDVDKLRFVPRVQRLNELEAKTRVKLNFLDQIAKFWELQGSSLKIPMVERKALDLYTLHRIVQEEGGMEQTTKDRKWAKVANRMQYPSSKSVGATLKAHYERILHPFEVYTSGKVLGPTPTSSGSGSTPVKLEDGGGTDYKAHEIPTRQQIAPPNETNTRRSKRFGNSNASCGLSGVTPTTKPSAGVFVKTETKEEFKRDLLSSFNAVNSGGSPLATGTTANTRGASQKKGGEPPALIVDPLMKYICHICNRGDVEESMLLCDGCDDSYHTFCLLPPLTSIPKGEWLCPRCVVEEVSKPQEAFGFEQAEREYTLQQFGQMADQFKQEYFRKPVHLVPTEMVEREFWRIVSSIDEDVTVEYGADLHTMDHGSGFPTKSSLYLLPGDQEYAESSWNLNNLPLLEDSILGHINADISGMNAPWMYVGMCFAAFCWHNEDHWSYSINYLHWGEPKTWYGVPGSCAEQFEETMKQAAPELFSSQPDLLHQLVTIMNPNILMNNRVPVFRTDQHAGEFVITFPRAYHAGFNQGYNFAEAVNFAPADWLKMGRECVNHYSMLRRFCVFSHDELVCKMALEPAKLTFGIATACYIDMAEMVDTEKKLRKSLLEWGVTRAERRAFELVNDDERHCQECNTTCFLSAVACECNDKLIVCLRHYTVLCGCAPEKHTLIYRYTLDEMPLMLQKLKVKAHSFERWLSRCRDIVDAHTPTSVTLQELQELCKEAETKKFPSSLLIDRLNAAAVEAEKCVTVIQQLGINKVRTRSDHNQEAAQYKLTMEELELFVQEIDNLCCIIDEGASVRELLVLGKQFVERSESQLQLSLESLEESELETLINEGSSLRIELQQLDLLQKRLKQCKWYKRSQGLRETSSKLTYQDVKNLLHIAAADLDPTDPYVDKEMRKLQQIGADIEAWESQAAKYFRRLTQQHELGEIEQFLKSASDINGQVPSHGLLKDALRKAREWLRAVEQLQQNNHVTYCHTLEAMIERGLNIPIQLEELSRMQGHLNSAHQWKDNTACAFLKKGTFYTLLEVLMPRSDAINIDSDLKPRFQDDFLKEKNPAEIVASFKHAEEQELLDMRELRRQNMNKNPMRDMFCLCKSEFRNLMFNCQLCRDWFHEDCVPPPSATNQNGIVNGGSGPGTNRPKWLCPSCVRSKRPRLETILPLLVQLQQLPIRLPEDEALRCLAERAMNWQDRARKALSSPDVSAAQEAIMAQQQQKRRSEGGAGVGNISSPRKPRRRGSLTKEASGSTESDADDDDDEDECRLRIVEDNFSNDEDEPRTAPATSTVNSDLLKLLSDSEIENLLDLMMEGDLLEVSLDETQELWRILETMPPTLLQAEAMERVVQYMQRQRQQHTNPLPTSGAEDSNDSLMVQNSPNSNSNSGGATGSASNSGRNKKRRSNDTGGNSAVPRKKQSTPKQTPGKKGSAAAARKSDAKASPAASTTPGADADAENKQANGGNTNSSTGSGGGNSATTTPTPGSTHKKRKRTSTTATNNNNNNNNNSTNNSNSSTNLNSNTTSGQGAATGGNNATGGQKKHAQRSQQAAQEDDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQIKPDDDYICIRCTKTVAIGTQGSGHSMSVASTTTPGKQRAVQSAR

Target ORF information:

RefSeq Version	NM_001298720.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster little imaginal discs (lid), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298720.1

ORF Insert Sequence:

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lid ( NM_001258980.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245909.1 Drosophila melanogaster little imaginal discs (lid), transcript variant D, mRNA.

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CloneID	OFa13853
Clone ID Related Accession (Same CDS sequence)	NM_078762.6 , NM_001298720.1 , NM_001258980.1 , NM_001258979.2 , NM_164671.1 , NM_001258981.2
Accession Version	NM_001258980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	little imaginal discs, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245909.1
CDS	124..5640
Translation	MSAKTEADNTTAANSGGGGVGSGTSSGGGASANGTATPARRLRTRNSTGNGTNSGSESVKKSNANDEPSTPVTPAGATGSHTHAPPGISPAVMERPMPSVPMNHASSSVSASKKYHNSCPHPTPTPAPTGHKKSVHTQPHSSNKFDQGKNEEFHFDTPPECPVFRPTTEEFKNPLAYISKIRSIAEKCGIAKILPPATWSPPFAVDVDKLRFVPRVQRLNELEAKTRVKLNFLDQIAKFWELQGSSLKIPMVERKALDLYTLHRIVQEEGGMEQTTKDRKWAKVANRMQYPSSKSVGATLKAHYERILHPFEVYTSGKVLGPTPTSSGSGSTPVKLEDGGGTDYKAHEIPTRQQIAPPNETNTRRSKRFGNSNASCGLSGVTPTTKPSAGVFVKTETKEEFKRDLLSSFNAVNSGGSPLATGTTANTRGASQKKGGEPPALIVDPLMKYICHICNRGDVEESMLLCDGCDDSYHTFCLLPPLTSIPKGEWLCPRCVVEEVSKPQEAFGFEQAEREYTLQQFGQMADQFKQEYFRKPVHLVPTEMVEREFWRIVSSIDEDVTVEYGADLHTMDHGSGFPTKSSLYLLPGDQEYAESSWNLNNLPLLEDSILGHINADISGMNAPWMYVGMCFAAFCWHNEDHWSYSINYLHWGEPKTWYGVPGSCAEQFEETMKQAAPELFSSQPDLLHQLVTIMNPNILMNNRVPVFRTDQHAGEFVITFPRAYHAGFNQGYNFAEAVNFAPADWLKMGRECVNHYSMLRRFCVFSHDELVCKMALEPAKLTFGIATACYIDMAEMVDTEKKLRKSLLEWGVTRAERRAFELVNDDERHCQECNTTCFLSAVACECNDKLIVCLRHYTVLCGCAPEKHTLIYRYTLDEMPLMLQKLKVKAHSFERWLSRCRDIVDAHTPTSVTLQELQELCKEAETKKFPSSLLIDRLNAAAVEAEKCVTVIQQLGINKVRTRSDHNQEAAQYKLTMEELELFVQEIDNLCCIIDEGASVRELLVLGKQFVERSESQLQLSLESLEESELETLINEGSSLRIELQQLDLLQKRLKQCKWYKRSQGLRETSSKLTYQDVKNLLHIAAADLDPTDPYVDKEMRKLQQIGADIEAWESQAAKYFRRLTQQHELGEIEQFLKSASDINGQVPSHGLLKDALRKAREWLRAVEQLQQNNHVTYCHTLEAMIERGLNIPIQLEELSRMQGHLNSAHQWKDNTACAFLKKGTFYTLLEVLMPRSDAINIDSDLKPRFQDDFLKEKNPAEIVASFKHAEEQELLDMRELRRQNMNKNPMRDMFCLCKSEFRNLMFNCQLCRDWFHEDCVPPPSATNQNGIVNGGSGPGTNRPKWLCPSCVRSKRPRLETILPLLVQLQQLPIRLPEDEALRCLAERAMNWQDRARKALSSPDVSAAQEAIMAQQQQKRRSEGGAGVGNISSPRKPRRRGSLTKEASGSTESDADDDDDEDECRLRIVEDNFSNDEDEPRTAPATSTVNSDLLKLLSDSEIENLLDLMMEGDLLEVSLDETQELWRILETMPPTLLQAEAMERVVQYMQRQRQQHTNPLPTSGAEDSNDSLMVQNSPNSNSNSGGATGSASNSGRNKKRRSNDTGGNSAVPRKKQSTPKQTPGKKGSAAAARKSDAKASPAASTTPGADADAENKQANGGNTNSSTGSGGGNSATTTPTPGSTHKKRKRTSTTATNNNNNNNNNSTNNSNSSTNLNSNTTSGQGAATGGNNATGGQKKHAQRSQQAAQEDDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQIKPDDDYICIRCTKTVAIGTQGSGHSMSVASTTTPGKQRAVQSAR

Target ORF information:

RefSeq Version	NM_001258980.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster little imaginal discs (lid), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258980.1

ORF Insert Sequence:

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lid ( NM_001258979.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245908.1 Drosophila melanogaster little imaginal discs (lid), transcript variant C, mRNA.

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CloneID	OFa13853
Clone ID Related Accession (Same CDS sequence)	NM_078762.6 , NM_001298720.1 , NM_001258980.1 , NM_001258979.2 , NM_164671.1 , NM_001258981.2
Accession Version	NM_001258979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	little imaginal discs, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258979.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245908.1
CDS	134..5650
Translation	MSAKTEADNTTAANSGGGGVGSGTSSGGGASANGTATPARRLRTRNSTGNGTNSGSESVKKSNANDEPSTPVTPAGATGSHTHAPPGISPAVMERPMPSVPMNHASSSVSASKKYHNSCPHPTPTPAPTGHKKSVHTQPHSSNKFDQGKNEEFHFDTPPECPVFRPTTEEFKNPLAYISKIRSIAEKCGIAKILPPATWSPPFAVDVDKLRFVPRVQRLNELEAKTRVKLNFLDQIAKFWELQGSSLKIPMVERKALDLYTLHRIVQEEGGMEQTTKDRKWAKVANRMQYPSSKSVGATLKAHYERILHPFEVYTSGKVLGPTPTSSGSGSTPVKLEDGGGTDYKAHEIPTRQQIAPPNETNTRRSKRFGNSNASCGLSGVTPTTKPSAGVFVKTETKEEFKRDLLSSFNAVNSGGSPLATGTTANTRGASQKKGGEPPALIVDPLMKYICHICNRGDVEESMLLCDGCDDSYHTFCLLPPLTSIPKGEWLCPRCVVEEVSKPQEAFGFEQAEREYTLQQFGQMADQFKQEYFRKPVHLVPTEMVEREFWRIVSSIDEDVTVEYGADLHTMDHGSGFPTKSSLYLLPGDQEYAESSWNLNNLPLLEDSILGHINADISGMNAPWMYVGMCFAAFCWHNEDHWSYSINYLHWGEPKTWYGVPGSCAEQFEETMKQAAPELFSSQPDLLHQLVTIMNPNILMNNRVPVFRTDQHAGEFVITFPRAYHAGFNQGYNFAEAVNFAPADWLKMGRECVNHYSMLRRFCVFSHDELVCKMALEPAKLTFGIATACYIDMAEMVDTEKKLRKSLLEWGVTRAERRAFELVNDDERHCQECNTTCFLSAVACECNDKLIVCLRHYTVLCGCAPEKHTLIYRYTLDEMPLMLQKLKVKAHSFERWLSRCRDIVDAHTPTSVTLQELQELCKEAETKKFPSSLLIDRLNAAAVEAEKCVTVIQQLGINKVRTRSDHNQEAAQYKLTMEELELFVQEIDNLCCIIDEGASVRELLVLGKQFVERSESQLQLSLESLEESELETLINEGSSLRIELQQLDLLQKRLKQCKWYKRSQGLRETSSKLTYQDVKNLLHIAAADLDPTDPYVDKEMRKLQQIGADIEAWESQAAKYFRRLTQQHELGEIEQFLKSASDINGQVPSHGLLKDALRKAREWLRAVEQLQQNNHVTYCHTLEAMIERGLNIPIQLEELSRMQGHLNSAHQWKDNTACAFLKKGTFYTLLEVLMPRSDAINIDSDLKPRFQDDFLKEKNPAEIVASFKHAEEQELLDMRELRRQNMNKNPMRDMFCLCKSEFRNLMFNCQLCRDWFHEDCVPPPSATNQNGIVNGGSGPGTNRPKWLCPSCVRSKRPRLETILPLLVQLQQLPIRLPEDEALRCLAERAMNWQDRARKALSSPDVSAAQEAIMAQQQQKRRSEGGAGVGNISSPRKPRRRGSLTKEASGSTESDADDDDDEDECRLRIVEDNFSNDEDEPRTAPATSTVNSDLLKLLSDSEIENLLDLMMEGDLLEVSLDETQELWRILETMPPTLLQAEAMERVVQYMQRQRQQHTNPLPTSGAEDSNDSLMVQNSPNSNSNSGGATGSASNSGRNKKRRSNDTGGNSAVPRKKQSTPKQTPGKKGSAAAARKSDAKASPAASTTPGADADAENKQANGGNTNSSTGSGGGNSATTTPTPGSTHKKRKRTSTTATNNNNNNNNNSTNNSNSSTNLNSNTTSGQGAATGGNNATGGQKKHAQRSQQAAQEDDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQIKPDDDYICIRCTKTVAIGTQGSGHSMSVASTTTPGKQRAVQSAR

Target ORF information:

RefSeq Version	NM_001258979.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster little imaginal discs (lid), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258979.2

ORF Insert Sequence:

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lid ( NM_164671.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723140.1 Drosophila melanogaster little imaginal discs (lid), transcript variant B, mRNA.

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CloneID	OFa13853
Clone ID Related Accession (Same CDS sequence)	NM_078762.6 , NM_001298720.1 , NM_001258980.1 , NM_001258979.2 , NM_164671.1 , NM_001258981.2
Accession Version	NM_164671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	little imaginal discs, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723140.1
CDS	114..5630
Translation	MSAKTEADNTTAANSGGGGVGSGTSSGGGASANGTATPARRLRTRNSTGNGTNSGSESVKKSNANDEPSTPVTPAGATGSHTHAPPGISPAVMERPMPSVPMNHASSSVSASKKYHNSCPHPTPTPAPTGHKKSVHTQPHSSNKFDQGKNEEFHFDTPPECPVFRPTTEEFKNPLAYISKIRSIAEKCGIAKILPPATWSPPFAVDVDKLRFVPRVQRLNELEAKTRVKLNFLDQIAKFWELQGSSLKIPMVERKALDLYTLHRIVQEEGGMEQTTKDRKWAKVANRMQYPSSKSVGATLKAHYERILHPFEVYTSGKVLGPTPTSSGSGSTPVKLEDGGGTDYKAHEIPTRQQIAPPNETNTRRSKRFGNSNASCGLSGVTPTTKPSAGVFVKTETKEEFKRDLLSSFNAVNSGGSPLATGTTANTRGASQKKGGEPPALIVDPLMKYICHICNRGDVEESMLLCDGCDDSYHTFCLLPPLTSIPKGEWLCPRCVVEEVSKPQEAFGFEQAEREYTLQQFGQMADQFKQEYFRKPVHLVPTEMVEREFWRIVSSIDEDVTVEYGADLHTMDHGSGFPTKSSLYLLPGDQEYAESSWNLNNLPLLEDSILGHINADISGMNAPWMYVGMCFAAFCWHNEDHWSYSINYLHWGEPKTWYGVPGSCAEQFEETMKQAAPELFSSQPDLLHQLVTIMNPNILMNNRVPVFRTDQHAGEFVITFPRAYHAGFNQGYNFAEAVNFAPADWLKMGRECVNHYSMLRRFCVFSHDELVCKMALEPAKLTFGIATACYIDMAEMVDTEKKLRKSLLEWGVTRAERRAFELVNDDERHCQECNTTCFLSAVACECNDKLIVCLRHYTVLCGCAPEKHTLIYRYTLDEMPLMLQKLKVKAHSFERWLSRCRDIVDAHTPTSVTLQELQELCKEAETKKFPSSLLIDRLNAAAVEAEKCVTVIQQLGINKVRTRSDHNQEAAQYKLTMEELELFVQEIDNLCCIIDEGASVRELLVLGKQFVERSESQLQLSLESLEESELETLINEGSSLRIELQQLDLLQKRLKQCKWYKRSQGLRETSSKLTYQDVKNLLHIAAADLDPTDPYVDKEMRKLQQIGADIEAWESQAAKYFRRLTQQHELGEIEQFLKSASDINGQVPSHGLLKDALRKAREWLRAVEQLQQNNHVTYCHTLEAMIERGLNIPIQLEELSRMQGHLNSAHQWKDNTACAFLKKGTFYTLLEVLMPRSDAINIDSDLKPRFQDDFLKEKNPAEIVASFKHAEEQELLDMRELRRQNMNKNPMRDMFCLCKSEFRNLMFNCQLCRDWFHEDCVPPPSATNQNGIVNGGSGPGTNRPKWLCPSCVRSKRPRLETILPLLVQLQQLPIRLPEDEALRCLAERAMNWQDRARKALSSPDVSAAQEAIMAQQQQKRRSEGGAGVGNISSPRKPRRRGSLTKEASGSTESDADDDDDEDECRLRIVEDNFSNDEDEPRTAPATSTVNSDLLKLLSDSEIENLLDLMMEGDLLEVSLDETQELWRILETMPPTLLQAEAMERVVQYMQRQRQQHTNPLPTSGAEDSNDSLMVQNSPNSNSNSGGATGSASNSGRNKKRRSNDTGGNSAVPRKKQSTPKQTPGKKGSAAAARKSDAKASPAASTTPGADADAENKQANGGNTNSSTGSGGGNSATTTPTPGSTHKKRKRTSTTATNNNNNNNNNSTNNSNSSTNLNSNTTSGQGAATGGNNATGGQKKHAQRSQQAAQEDDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQIKPDDDYICIRCTKTVAIGTQGSGHSMSVASTTTPGKQRAVQSAR

Target ORF information:

RefSeq Version	NM_164671.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster little imaginal discs (lid), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164671.1

ORF Insert Sequence:

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lid ( NM_001258981.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245910.1 Drosophila melanogaster little imaginal discs (lid), transcript variant E, mRNA.

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CloneID	OFa13853
Clone ID Related Accession (Same CDS sequence)	NM_078762.6 , NM_001298720.1 , NM_001258980.1 , NM_001258979.2 , NM_164671.1 , NM_001258981.2
Accession Version	NM_001258981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	little imaginal discs, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258981.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245910.1
CDS	246..5762
Translation	MSAKTEADNTTAANSGGGGVGSGTSSGGGASANGTATPARRLRTRNSTGNGTNSGSESVKKSNANDEPSTPVTPAGATGSHTHAPPGISPAVMERPMPSVPMNHASSSVSASKKYHNSCPHPTPTPAPTGHKKSVHTQPHSSNKFDQGKNEEFHFDTPPECPVFRPTTEEFKNPLAYISKIRSIAEKCGIAKILPPATWSPPFAVDVDKLRFVPRVQRLNELEAKTRVKLNFLDQIAKFWELQGSSLKIPMVERKALDLYTLHRIVQEEGGMEQTTKDRKWAKVANRMQYPSSKSVGATLKAHYERILHPFEVYTSGKVLGPTPTSSGSGSTPVKLEDGGGTDYKAHEIPTRQQIAPPNETNTRRSKRFGNSNASCGLSGVTPTTKPSAGVFVKTETKEEFKRDLLSSFNAVNSGGSPLATGTTANTRGASQKKGGEPPALIVDPLMKYICHICNRGDVEESMLLCDGCDDSYHTFCLLPPLTSIPKGEWLCPRCVVEEVSKPQEAFGFEQAEREYTLQQFGQMADQFKQEYFRKPVHLVPTEMVEREFWRIVSSIDEDVTVEYGADLHTMDHGSGFPTKSSLYLLPGDQEYAESSWNLNNLPLLEDSILGHINADISGMNAPWMYVGMCFAAFCWHNEDHWSYSINYLHWGEPKTWYGVPGSCAEQFEETMKQAAPELFSSQPDLLHQLVTIMNPNILMNNRVPVFRTDQHAGEFVITFPRAYHAGFNQGYNFAEAVNFAPADWLKMGRECVNHYSMLRRFCVFSHDELVCKMALEPAKLTFGIATACYIDMAEMVDTEKKLRKSLLEWGVTRAERRAFELVNDDERHCQECNTTCFLSAVACECNDKLIVCLRHYTVLCGCAPEKHTLIYRYTLDEMPLMLQKLKVKAHSFERWLSRCRDIVDAHTPTSVTLQELQELCKEAETKKFPSSLLIDRLNAAAVEAEKCVTVIQQLGINKVRTRSDHNQEAAQYKLTMEELELFVQEIDNLCCIIDEGASVRELLVLGKQFVERSESQLQLSLESLEESELETLINEGSSLRIELQQLDLLQKRLKQCKWYKRSQGLRETSSKLTYQDVKNLLHIAAADLDPTDPYVDKEMRKLQQIGADIEAWESQAAKYFRRLTQQHELGEIEQFLKSASDINGQVPSHGLLKDALRKAREWLRAVEQLQQNNHVTYCHTLEAMIERGLNIPIQLEELSRMQGHLNSAHQWKDNTACAFLKKGTFYTLLEVLMPRSDAINIDSDLKPRFQDDFLKEKNPAEIVASFKHAEEQELLDMRELRRQNMNKNPMRDMFCLCKSEFRNLMFNCQLCRDWFHEDCVPPPSATNQNGIVNGGSGPGTNRPKWLCPSCVRSKRPRLETILPLLVQLQQLPIRLPEDEALRCLAERAMNWQDRARKALSSPDVSAAQEAIMAQQQQKRRSEGGAGVGNISSPRKPRRRGSLTKEASGSTESDADDDDDEDECRLRIVEDNFSNDEDEPRTAPATSTVNSDLLKLLSDSEIENLLDLMMEGDLLEVSLDETQELWRILETMPPTLLQAEAMERVVQYMQRQRQQHTNPLPTSGAEDSNDSLMVQNSPNSNSNSGGATGSASNSGRNKKRRSNDTGGNSAVPRKKQSTPKQTPGKKGSAAAARKSDAKASPAASTTPGADADAENKQANGGNTNSSTGSGGGNSATTTPTPGSTHKKRKRTSTTATNNNNNNNNNSTNNSNSSTNLNSNTTSGQGAATGGNNATGGQKKHAQRSQQAAQEDDEEECRAENCHKPTGREVDWVQCDGGCNEWFHMYCVGLNRSQIKPDDDYICIRCTKTVAIGTQGSGHSMSVASTTTPGKQRAVQSAR

Target ORF information:

RefSeq Version	NM_001258981.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster little imaginal discs (lid), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258981.2

ORF Insert Sequence:

ATGTCCGCCA AAACTGAGGC GGACAACACG ACGGCCGCCA ACTCCGGCGG CGGAGGAGTC 
GGGAGCGGCA CCTCATCCGG CGGGGGCGCA TCCGCGAATG GAACAGCAAC TCCGGCACGT 
CGTCTGCGTA CCCGAAACTC AACGGGCAAC GGGACTAATA GTGGAAGTGA GTCCGTAAAG 
AAATCCAATG CTAACGACGA GCCCTCGACC CCAGTCACCC CGGCAGGAGC AACAGGATCT 
CACACCCATG CTCCGCCAGG CATTTCTCCG GCGGTAATGG AACGGCCTAT GCCCAGTGTT 
CCCATGAATC ACGCCAGCAG CAGTGTGTCA GCCAGCAAAA AGTACCATAA TAGTTGTCCT 
CATCCCACGC CCACGCCTGC GCCCACTGGG CACAAGAAGT CGGTCCACAC GCAGCCGCAC 
AGCAGCAACA AGTTTGACCA GGGAAAGAAC GAGGAGTTCC ACTTTGACAC GCCGCCAGAG 
TGCCCTGTGT TCCGGCCCAC AACTGAGGAG TTCAAGAATC CACTGGCCTA CATCAGCAAG 
ATCCGCTCCA TAGCTGAGAA GTGTGGAATC GCAAAGATCC TGCCGCCGGC AACCTGGTCG 
CCGCCGTTTG CAGTGGACGT GGACAAGCTA CGCTTTGTGC CACGTGTCCA GCGGCTGAAT 
GAGCTCGAGG CAAAGACGCG CGTCAAACTC AACTTCCTTG ATCAGATCGC AAAGTTCTGG 
GAGCTGCAAG GCTCTTCACT AAAGATCCCA ATGGTGGAGC GAAAGGCTCT CGACTTGTAC 
ACACTGCACC GCATCGTTCA GGAGGAGGGG GGCATGGAGC AAACCACAAA GGATCGCAAG 
TGGGCCAAGG TGGCCAACCG GATGCAGTAC CCCTCAAGCA AAAGTGTTGG CGCAACCCTG 
AAGGCACACT ACGAACGCAT CTTGCACCCC TTTGAGGTAT ACACTTCGGG AAAGGTTCTA 
GGACCCACAC CAACGTCGTC TGGATCTGGT AGCACACCCG TCAAATTGGA GGACGGAGGT 
GGAACCGATT ACAAAGCCCA TGAAATTCCC ACACGGCAGC AGATTGCGCC GCCCAACGAG 
ACAAACACAC GTCGCTCCAA GCGTTTCGGA AATTCCAATG CCAGCTGCGG CCTGAGTGGT 
GTTACACCCA CAACAAAGCC ATCAGCGGGC GTATTTGTCA AGACGGAGAC CAAGGAAGAA 
TTTAAGAGGG ATCTTCTCAG CAGTTTCAAT GCTGTCAACA GCGGCGGATC GCCATTAGCC 
ACAGGTACTA CGGCAAACAC TCGCGGAGCC AGCCAGAAGA AGGGAGGAGA GCCTCCAGCA 
CTTATCGTAG ACCCACTGAT GAAGTACATT TGCCACATCT GCAATCGCGG CGACGTCGAG 
GAGTCAATGC TGCTTTGCGA TGGCTGCGAC GACAGCTACC ACACCTTTTG CCTGTTGCCG 
CCTCTGACCA GCATACCCAA AGGCGAATGG CTTTGCCCGC GCTGCGTCGT CGAGGAAGTA 
AGCAAACCGC AGGAGGCGTT TGGTTTTGAA CAGGCAGAGA GGGAATACAC TCTGCAACAA 
TTTGGACAAA TGGCTGACCA GTTCAAGCAA GAATACTTCC GCAAGCCGGT GCACTTGGTG 
CCCACTGAGA TGGTGGAGCG CGAGTTTTGG CGCATCGTGT CATCTATCGA TGAAGACGTT 
ACCGTTGAGT ATGGGGCGGA CTTGCACACA ATGGATCACG GCTCTGGTTT TCCCACTAAG 
AGCTCGTTAT ATTTGTTGCC GGGCGACCAA GAGTATGCAG AGTCAAGCTG GAACCTTAAC 
AACTTGCCGT TGCTGGAGGA TTCCATTCTT GGTCACATCA ATGCCGACAT TAGCGGCATG 
AACGCTCCGT GGATGTATGT GGGCATGTGC TTCGCAGCCT TCTGCTGGCA CAACGAGGAC 
CACTGGAGCT ACTCCATCAA CTATCTTCAC TGGGGAGAAC CTAAAACCTG GTACGGTGTT 
CCGGGGTCCT GTGCTGAGCA GTTTGAAGAG ACCATGAAGC AGGCAGCTCC AGAACTTTTT 
TCATCACAGC CCGATCTGCT TCACCAGCTG GTGACCATTA TGAACCCGAA CATTTTGATG 
AACAATCGTG TACCGGTATT CCGAACCGAT CAGCATGCAG GCGAGTTTGT GATCACCTTT 
CCCAGGGCCT ATCACGCAGG CTTTAACCAA GGCTATAACT TCGCGGAGGC CGTCAATTTT 
GCACCTGCCG ATTGGCTGAA GATGGGGCGC GAGTGTGTTA ACCACTATTC CATGCTGCGA 
CGTTTCTGTG TCTTTTCGCA CGACGAGCTC GTTTGCAAGA TGGCCCTGGA GCCAGCGAAA 
CTCACATTTG GCATCGCTAC TGCCTGCTAC ATCGACATGG CCGAAATGGT CGACACGGAG 
AAGAAACTGC GCAAATCGCT TTTGGAGTGG GGCGTTACCC GCGCAGAGCG TCGCGCCTTC 
GAGCTAGTAA ACGACGATGA GCGCCATTGC CAAGAGTGCA ACACCACGTG CTTCCTGTCG 
GCGGTGGCCT GCGAGTGCAA CGACAAGTTG ATTGTCTGTT TGCGCCACTA TACGGTACTC 
TGCGGCTGTG CTCCTGAGAA GCACACTCTG ATATATCGTT ATACACTGGA CGAGATGCCG 
CTGATGCTGC AGAAACTTAA GGTGAAGGCG CACAGCTTCG AGCGATGGCT TTCACGTTGC 
CGTGACATAG TCGATGCACA TACACCCACC TCGGTGACCC TGCAGGAGCT GCAGGAATTG 
TGCAAGGAGG CCGAGACGAA AAAGTTTCCC TCCTCGCTGC TCATCGATCG CCTTAATGCT 
GCGGCCGTTG AGGCAGAGAA ATGTGTCACA GTCATACAGC AACTGGGTAT CAATAAGGTG 
CGAACTCGCT CGGACCACAA CCAGGAGGCT GCTCAGTACA AGCTAACCAT GGAGGAGCTA 
GAGTTGTTCG TTCAAGAAAT CGATAATCTC TGCTGCATCA TTGACGAGGG CGCTTCGGTG 
CGAGAGCTGC TTGTTTTGGG AAAACAGTTT GTGGAGCGGT CCGAGAGCCA GCTGCAATTA 
TCGCTGGAGT CTCTGGAGGA GAGTGAGCTG GAAACGTTAA TAAACGAAGG CAGCTCCTTA 
CGCATCGAAC TGCAACAGCT CGACCTACTG CAAAAGCGAC TGAAGCAGTG CAAGTGGTAC 
AAGCGCTCGC AGGGATTGAG GGAGACCAGC TCGAAGCTGA CCTACCAAGA CGTGAAGAAC 
CTGTTACACA TTGCGGCTGC TGACCTTGAT CCCACGGATC CGTATGTCGA CAAGGAGATG 
CGAAAATTGC AACAGATCGG TGCAGATATC GAAGCCTGGG AGTCGCAGGC GGCCAAGTAT 
TTCCGGCGAC TGACCCAACA GCACGAACTG GGCGAAATTG AGCAGTTCTT GAAATCGGCC 
TCCGACATCA ACGGTCAAGT GCCTTCGCAT GGACTGCTGA AGGATGCACT ACGAAAGGCG 
CGTGAGTGGC TACGGGCCGT TGAGCAGCTA CAGCAAAACA ACCACGTCAC ATATTGCCAC 
ACACTGGAGG CAATGATCGA GCGCGGCTTG AACATACCCA TCCAGCTGGA GGAGCTGAGC 
CGAATGCAGG GACACCTAAA TAGTGCACAT CAATGGAAGG ACAATACAGC GTGCGCCTTC 
CTCAAGAAGG GCACCTTCTA TACGCTCTTG GAGGTTCTCA TGCCCCGGTC GGACGCCATC 
AACATAGACT CGGATCTCAA GCCGCGCTTC CAGGATGATT TTCTGAAAGA GAAAAATCCG 
GCGGAGATTG TGGCCAGCTT CAAGCACGCT GAGGAGCAGG AGCTGCTCGA TATGCGCGAA 
CTTCGGCGGC AAAATATGAA CAAGAATCCC ATGCGCGATA TGTTCTGCTT GTGCAAGTCC 
GAGTTCCGAA ACTTAATGTT CAATTGTCAG TTGTGCCGCG ATTGGTTTCA CGAGGATTGC 
GTCCCACCGC CATCAGCCAC AAATCAAAAT GGAATTGTTA ACGGCGGATC AGGACCAGGT 
ACCAATCGAC CCAAGTGGCT ATGTCCGAGC TGTGTGCGCT CGAAGAGACC GCGCCTAGAA 
ACCATCCTTC CGCTACTTGT CCAGCTGCAG CAACTGCCCA TCCGTCTGCC CGAGGACGAA 
GCGTTGCGTT GCCTGGCGGA GCGTGCGATG AACTGGCAGG ATCGGGCCAG GAAAGCTCTT 
AGCAGTCCTG ACGTCAGTGC GGCTCAAGAA GCGATTATGG CGCAGCAGCA GCAGAAGCGG 
CGCTCCGAGG GTGGAGCAGG GGTGGGAAAT ATCAGCAGTC CACGGAAGCC ACGTCGACGG 
GGGAGCCTGA CGAAAGAGGC AAGTGGTTCA ACAGAGTCCG ATGCCGACGA CGATGACGAC 
GAGGACGAAT GTCGTCTTCG AATCGTCGAA GACAACTTTA GTAACGATGA AGATGAGCCT 
CGTACCGCCC CCGCGACCTC CACCGTCAAC TCCGATCTCC TCAAGCTGCT GTCCGACAGT 
GAAATTGAAA ATTTGCTTGA CCTGATGATG GAGGGCGACC TGCTAGAGGT ATCCTTAGAT 
GAAACGCAGG AGCTGTGGCG CATTCTAGAA ACGATGCCGC CCACGTTGCT GCAGGCGGAG 
GCCATGGAGC GTGTTGTCCA GTACATGCAG CGTCAGCGGC AACAGCATAC AAATCCGCTG 
CCAACATCCG GCGCCGAAGA CTCTAACGAT AGTCTGATGG TACAAAATAG TCCCAATAGC 
AACAGCAATA GTGGCGGGGC AACGGGTTCT GCGAGCAACA GCGGACGCAA CAAGAAGAGG 
CGCTCCAACG ACACCGGCGG CAACTCGGCA GTGCCGCGCA AGAAGCAGAG CACACCGAAG 
CAGACACCGG GCAAGAAGGG ATCAGCGGCA GCGGCACGCA AGAGCGATGC CAAGGCATCG 
CCAGCAGCAT CCACCACTCC GGGTGCGGAT GCCGATGCCG AGAATAAGCA GGCCAACGGC 
GGTAATACGA ACTCCAGTAC AGGATCAGGT GGTGGCAATT CTGCGACGAC GACGCCCACA 
CCCGGCTCGA CGCACAAAAA GCGAAAGCGC ACCTCAACCA CAGCCACCAA TAATAACAAC 
AACAATAATA ATAACAGCAC GAACAATAGC AACAGCAGTA CCAATTTGAA CAGCAACACC 
ACCAGCGGCC AGGGAGCAGC TACTGGAGGC AATAATGCGA CTGGCGGTCA GAAGAAGCAC 
GCGCAACGGT CTCAACAGGC GGCGCAGGAG GACGACGAGG AGGAGTGCCG GGCGGAGAAC 
TGCCACAAGC CAACCGGGCG AGAGGTGGAC TGGGTGCAGT GCGACGGCGG CTGCAACGAA 
TGGTTCCACA TGTACTGTGT GGGCCTCAAT CGCAGCCAGA TAAAGCCCGA CGACGACTAT 
ATCTGCATTC GATGCACAAA GACAGTTGCC ATCGGAACAC AGGGCTCCGG TCACAGTATG 
AGCGTGGCGT CCACGACGAC GCCGGGCAAG CAGCGGGCGG TGCAATCGGC GCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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lid cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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