Nup205 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nup205
Entrez Gene ID	33002
Full Name	Nucleoporin 205kD
Synonyms	CG11943,Dmel\CG11943,Ov4,anon-EST:Posey281,nup205
General protein information	Preferred Names Nucleoporin 205kD Names CG11943 gene product from transcript CG11943-RB CG11943-PA CG11943-PB Nup205-PA Nup205-PB nucleoporin 205 nucleoporin 205kD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18F4-18F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_134518.4	NP_608362.2	nucleoporin 205kD, isoform A
NM_167687.3	NP_728308.1	nucleoporin 205kD, isoform B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NUP205	XP_416176.2
Arabidopsis thaliana (thale cress)	AT5G51200	NP_199933.5
Pan troglodytes (chimpanzee)	NUP205	XP_527899.3
Rattus norvegicus (Norway rat)	Nup205	NP_001102090.1
Drosophila melanogaster (fruit fly)	Nup205	NP_728308.1
	NUP205	XP_001105911.1
Bos taurus (cattle)	NUP205	NP_001124403.1
Mus musculus (house mouse)	Nup205	NP_081789.1
Danio rerio (zebrafish)	nup205	NP_001003859.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008090	XP_317367.4
Xenopus tropicalis (tropical clawed frog)	nup205	NP_001135596.1
Homo sapiens (human)	NUP205	NP_055950.1
Canis lupus familiaris (dog)	NUP205	XP_005629638.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nup205 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup205 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02191	NM_167687.3 Latest version!	Drosophila melanogaster nucleoporin 205kD, transcript variant B (Nup205), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa02192	NM_134518.4 Latest version!	Drosophila melanogaster nucleoporin 205kD, transcript variant A (Nup205), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nup205 ( NM_167687.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728308.1 Drosophila melanogaster nucleoporin 205kD, transcript variant B (Nup205), mRNA.

Price & Availability↑

CloneID	OFa02191
Clone ID Related Accession (Same CDS sequence)	NM_167687.3
Accession Version	NM_167687.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 205kD, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167687.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGGTG AGTGCCTGGC CAACGAGCAG ATGTGCGTGG GTCGCCAAAT CACTAATGCC 
ACCCATGCGT CCACAGCTGT AATCGAGGAC ATGTGGACGC CGTACAAGCA TTTGTACAAC 
ACTTTTCAGA CCGCGGTGTC CAATCGCAGT GGCTTCACCT CGGATCTGGA GCAGTGCCTC 
AAGAAGTACA AGCACAACTT CAGCAACTTG CTGCGCAATC CGCCGAGAAG CGAGAAGAGC 
CGCAATCTCC TACGAAATGC CCTCAACGAA GGCGTCCCGA TGCAGGGGCA TTCGCGAAAG 
ACAAAGATGT CACAGGACCT GGCCGACGAG GCGGTCATTC TGTCCGACAT GTTCGATCTG 
GACGAGGGTT TCGCCGTGGA GCTGCTGTGC ACCGCACAGC GCCAGCAGAA GCATCATCCC 
GGCCTGTCCA GAGGCCTTGT CGCGGTGCTG CTCTACTACG ATGGACGAAA GGCCATCAGC 
TGCACGCTTC GCGACATGTT CCAGGTGGTT AGCGGCGTCT CGTGGAACAC CGAACTGCCC 
AAGGAGATCA CCGGTCTGGT CACTAACTAT GCCGAGAGCC TTGTCGACGG CTCTGGGATA 
CTGGGACGCC TGCTGCAGCT GCTCGAGGAG ATGGATGTCG ACAAGGAGTG CGCTATGCTG 
ACCACAAACC GCGCGTTTGG CTCCAAGAAG CATCAGAACC AGGTGCTCGG CCTATACGAG 
GACATTCAAC AGGCATTGGC AATGGCCCTG TTCAATTGGT CGGCACAACG CGGCCTGCCG 
CGACACATTG CCATTCGGCT GATGCACCAA TTGGCCAACC GAAAGAACCA CGACGCGGGC 
GGCAACATGG ACGACGTGAC CCTGATCATG CTGATGGCTC TGCTGTATGC GTACGATACC 
TCCATGCTGC TGGTCACCGA GGAACCCAAT GAGCACACAA CCCGACTTCC GATCTTCAGC 
GATCGCGAGT TCGCCGAGTG CTTCCTGGAG GAGCTGTACG CGCAGAGCAG CTGGCAAGCA 
CCGCGCCTGA ATGCCATCAT TGCCTACAGC TTCGGACTAA CGCTGGCCAG CCTGCGGCAT 
GCACCTTTGC AGCTTCAGGC CACCGCCATC TCGATCATCA ATCGCGATGA GATGCTCATC 
GACGAGGCAC TGGGAGCGCA GGTATTCGTC TTCTTCCACA GCTTGCTGCT CGAGAAGGAT 
CTGGTCTATA GCACGCAGTT CTTCTACCGC CGCGTCCATC TGCTCATCAC GGACTTCATC 
GACTTCATGA ACTCCAAGGT GTCGGAGCTG CGCGGCCGAG CAGACGAGTC GTCACACACG 
ATCATCAGCT TCCTAAACGA GGGTCTGGAA CCGCCGCCCA ACTTGGACTC CAACTTTGAG 
CTGCTCATGC TGTGCGTGGC CAAGATGCAC GGCGATCCGC GCGTCACCAT CAGGCTGTGC 
AACGAGTACT GGGGCCCCGG CGATCCCAAC GGCTCTACGG CATTCAAGAA CACCTCGCGC 
TCGGTGTCGC TGTTCAAGTT CATTAGCTTG GCCAGCGACC TGCTGCCACA GACGCTGTTC 
AAGGCCTACC TCAAGATGAT ATCCGGACTT ACACGGACCG ATTTCTCGGC TCGATGCGCC 
TTCAACATGC TTAGGGTGCC CCAGATGGCC ACCGGCGGCA AGTATGCGGT TAGCTGGGAT 
CACTTCTTCA CCACCCTGGG CAACTACTAT ACTTCGATGC GCAACGACTT TAACACCAAC 
ATCGGCATGA GCGGCGAGAC CATTTACCGC ACCCGATCGA CACCAAAGGC CATTACGCAG 
CGAGAGGCGG AGCATCTGGT GGCCGTAATG GGCATCATAC AGGCGGTGGC GGAACACGAC 
GAGGTCTCGC GCATCATGAT CTGTGACCAT CCCAATTGGC AGACGCCGCA GGTGCTCCTG 
GGCCTGGTTG CGTGCGCCAC GCCGCTTTTT TTGAAGGCGG AGATTCTGCA CACGCTGGCT 
GCCCTGGCCA AATCGAAGGA GACGGCGCGC GTCATCTGGT TCCACCTGGA GGCCTCGCAG 
ATTATACCCA CTGTGCCCGT GTCTAGAAGC TATGCCCAGT GCAGTTTGTT GGAGGAGATG 
GAACAAATCG AGAGCCGTTC GGAGCAGTAT AACCTGACGC GCGGCATCCT CCAATTGCTC 
TACACACTGA TGACCACCAA TCTGCCGAAG AGTTTGGGCG CCGGGCAACG GACACCGGCC 
TACGAAGGCT ATCTAAAGTC CATGATAAAT TCGGTGTTGC TCAAGTTTTA CAATCGCGCG 
TACAAGGTTC CCTCCGAGAA GTGGCAAGTT GGCGCCCAGT GCCTGAAACT GCTCTACTAC 
CTACTGGCCA CCTATCGGCC GTCCGCAATG GATTTTCTGG AGACAGTCGA TGAACCTCCC 
TATCCGGGCT TTCACGTTAT GATGCAGCTG CAGCTGAAGT CCGAAATGTT GCAGCTGCTG 
CTGTGCATTG TGGAGGAGGT GCGTGAGCGG CTGGACAACT ACAATCGCTT TCAGGGCAAG 
AAGCTACTCG AGGAGTGCTC ATTGTACGCA CTGCTGATCC TGGAAGCCGC ATTGGCGAAA 
CAGAACGCCT TCTTTGAGGC CCACTCGGCG GCCAATTGCC CCATTTTGCT GTCGGGCCTG 
AATCGCATGC TCCTGGATCT GAATCCACGC AGTCGCAAGC CCGATCACGT GCTGAATATC 
ATTAAGTATG TAACCTATAA TAGCTGGCTG CCGCGGCACG CCCTGGCCGC CATTAAGATT 
CTGGCTTCGG TCACCCAGCT GCCGAATGTC TCCACCCAGA TTCTCAGCAT GTACGGTCAG 
GGCAGCAATG AGAAGCTCGA GATTCGTCAG GGTTTCGTCG AGTGCCTGGA AATGGAGGTG 
TGCGTGGGCA AGCACGACGA CGATTTGTTG GACCAACTGG CCCTCAACAA TCACGTTCCG 
TATCTGGGAT TCGGCGATGA TCTGGACAAT GAGCGAGAGA TGAGCGGGGA ACGCGAATAT 
TCAACTATTG AATCGCAACT GGAGCTGCAG GCGGGGGATG GCGCGCTGGA GAGCAAACCG 
GCTAGCATTG AGCTGCAACT GAAAGAGGCC ATTATCAAGC TGTTCGAGAT GAATCTTAGC 
CAGCAGTTGC CCAACTTTGT GTACTTCCTG CTCGGCGTGG ATGTGCTGCG CGAATTTATG 
GCCAACGGAC GGCAGCACCT GGCCATCGAG ATGCAAAGCT CGTGCGTGAA CTCCGTAGTG 
CTGTTGCTGG AAAAGTACAT GGATAAACAG CGTCACAGCG ATAAGTACTG CGAGCACACG 
GCGAGAATCG TGGAGCGCAT TTACCATCTG TTCCATGGAC TCTGCGCCAA TCGCCGCACC 
GCGGAGACGA TACTGCGCTA CTTCCGGCTA ACGTGCAGCG ACTTCCTGCT GCGTCACCTA 
CGCTCGCTGC CCTTCCGGCA GCATCGCGAG GATCATGTGC TGCACGCCAT GGGCCACCTG 
CTCAACTGCG TCTCTATCGA TGTCAAACTG GCGGCCAAAC ATGGCCAGAT GACGCGCTTC 
AACCAGATGT GCGACATTTT GCTAATTGGA AACGGAATGG AGCGTTCATC CCATGGCCTG 
TCGATGGAAT TGGGCCACAG CCTGATTTCA CAGCCGTCGT CGTCTTTCCT CGCCATGGAT 
GTGCTACCGG CCGGTGGATC GATAAGTGCC GTGGCACATG GGGCTGGTTC GGCGTCTCTC 
GGAGCTCCAA ACACTGGGGT CAAATCACTT AAGCCATCGC TGCTGCAGGA GACATCGCAG 
GGACTGCATG TCACCCGACT GCTAGATATT CTCGTCCTGG AGGCGGGCAC GTTGAGCCAG 
CCGCAGCTGG AGTTCTTCGA CGGGCATCTG ATCACTCAAC TGCTGCGCGA CTGCGAGGCA 
TCCGCCGAAG CTGGCGCCAA TTCCAGGGCC AACTTGATCA ACGTGCGCAA ACTGTACTAC 
ATTCTCACCG ACGAGCTGTA CATGGTGCAG AGCATCATTG CCAGCGGCCA GCGGAAGGCC 
ATTTCCACGG AAATCATGCT GCTGCTCAAT CACGCCGTCA ACATAAATCG CGTGCGGACA 
CAGCGCTGCG CCACGCTGGC CTTCATGGCA GCATGGGGTC AACTGGTGCA GGTGCTCTTT 
AGCAACATGC CCGATGCCGT GCTTCCGGCG ACGCAGCGTC GCCAGCACAT CATCGATATC 
GTCGAGAAGG TACTCATCAA GGTGCAGCCC ATACAGCCCA TCATCGAGAT ATCCGTACAG 
GTGAGCGAGA CGGTGCTGCT GCTGCTTGCC AATTTGCGCT ACTGCTGCTA CCAGGCCGAG 
GACCAGAGCC CCGAGGATCT GGCCGCCGAG GATTCGCTCA GCAATGGCAA TGGCAACGCC 
ATCGGCAACG ATTCGCAGGT GAACGCATTG TGTCTGGGCC AGAGGGCAAT CGGAACTGGT 
TCGGATGGCG GCAGTGGAGG TGGACGGGAT ATCGGTTCCG GTGGCAACAG CAGCAATCTG 
CGATTCATTC TCAAGAACCT CGTCCAGTGG ATCATGATCA GTGAGGTGAA GTCGCAGAAG 
CTGCGCATCA ATCTGTACAG TTCGCTGCTG AATTGCCTGC GGATCGCGAA GCGACTGCGC 
ACCGACGAGC ATCTGGAGTA CCAGGAGACA TTGATTTCGC GCCAGGAGAG TGCACGCACG 
TACAACAAGG AGCAGCGGCG CGACGATCGC CTGCGGCTGA AGGCCATGGC GGCGGAAGTG 
ATTGGCACGT TCGGCGAGAA GCTGATCGAC ACCATCTGCC ACGATGCGGT GACCGGCCAC 
GATGTGTGCC GCATGCTGGC GCTGGCCTGC CTGGACATGA TCTCCGAGCT GCAGGCGGTG 
AGCACGCTAT GCGACTTTGT GGCATCGCGT GGCTACCTCA AGCACATACT GGAAAGCCTT 
GATCAGTCCA GTACGGCGCT TTGCGGGATT CTGCAGCCCG TGCCGGATCA CCTGAGGCCG 
CTCTACGTCT ACGAGTCGCG CATGGCCTTC CTCACGCGCA TGGCGAACAG CAATGTGGGA 
GCCCGGCTCC TCCTCACCGA GCAGGCGCTG GGCGTGCTTT CGAATATGAA GGTCTATGAC 
CAGCAGCCCG ACCTGAAGTC CAGCGAACTG AATCGTAACG AGCCGCAGAC CTTCCTCCCG 
TCCATCGATG ACCGCTTCCG GTCGATCCTC CTGCCGGCGC TGAGCCTCTG CGATGCCATT 
GTCAACTCGC TGGGTCCGCG CAACAACTCC GCCGCCGTGC AGGTGCTTAA CTTTCTGTTC 
GCCCACATCG ACATGGTCGA GGCCATGTTG CGCTCGGCCA CGCCGTATAT GGACCTCGGC 
CACCTGCAGC AGGTGGCGGT CATTAGCAAT CTGTTTGCCC GCGCCTCCAC GCACGAGTTG 
ACCGCGCTGG AGGATAGCGT GCAGCTGGAT TTGCGAAATC GCCTGGGCCG CGTGCAGCAG 
CTGATGATCG TGGTGTTCGG ACGCTTTTGC GTAAGCGAGC CCACCATTCG TCGCATGCTG 
CAGCAGGATG AGGAGCAGCA GACCAACCCC ACCGATGACT CGAAGAGGCT ACGTGTCAAG 
TATTTTCTGG ATATAGCAGC CAACGTGTCG CTCTACTGCC GCAACGTGGT CACCAGCCAT 
TCGAAGGACA GCATGACCTC CAAATACTTG CTGACCACCG TGATCAATGA TGTAACGCTG 
CTCACTGGCA AAATGTCCAG CAAAAAGCTG ACGACCATCA TGCACACCAT CCTCAATCAG 
CTGAAGGGCT CCATCGGCTA CTACCTTTCG CAGAAGTCGA TCGCCGACAA TCTCCTCCAG 
CAACGCGCCT CCCTGCCCAA CATCAGCTTT GGGCCCAACG GTAAACAAAG CTACATAGAA 
CTGAGCCAGC GATACAACGA GAAGCGCAGC GAACTGAGGC AGGCCGTCTT CATTGCCGAG 
CAGAATCTGT ATCTGCTGTG GATCCATCTG GACTTCTATC TGCGCAACAC CATCGACTAC 
GCCAACGAAA ACAGGAACGC CATCAACGAG AGCAACATGG ACGACGACAA TGACATGTCC 
GTGCTGAACG CCTCGCAGGA AGAGATCGTG CAGCTCAAGC AGCTGCTCAT CTCCACATTC 
AACGAGACAT TCTGCACGCA GCTAATCAAC GCCAGCGAGG ATTACTCGAT TAAGTGCAAA 
GGCTTCAATG GCTCGCTGCT GCGACGCATT AAGGCGCTCG TCCAGTTCGC CCCCATCACT 
GCCAACGATG TCAATTCCAG CTTCGATTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728308.1
CDS	135..6407
Translation	MDGECLANEQMCVGRQITNATHASTAVIEDMWTPYKHLYNTFQTAVSNRSGFTSDLEQCLKKYKHNFSNLLRNPPRSEKSRNLLRNALNEGVPMQGHSRKTKMSQDLADEAVILSDMFDLDEGFAVELLCTAQRQQKHHPGLSRGLVAVLLYYDGRKAISCTLRDMFQVVSGVSWNTELPKEITGLVTNYAESLVDGSGILGRLLQLLEEMDVDKECAMLTTNRAFGSKKHQNQVLGLYEDIQQALAMALFNWSAQRGLPRHIAIRLMHQLANRKNHDAGGNMDDVTLIMLMALLYAYDTSMLLVTEEPNEHTTRLPIFSDREFAECFLEELYAQSSWQAPRLNAIIAYSFGLTLASLRHAPLQLQATAISIINRDEMLIDEALGAQVFVFFHSLLLEKDLVYSTQFFYRRVHLLITDFIDFMNSKVSELRGRADESSHTIISFLNEGLEPPPNLDSNFELLMLCVAKMHGDPRVTIRLCNEYWGPGDPNGSTAFKNTSRSVSLFKFISLASDLLPQTLFKAYLKMISGLTRTDFSARCAFNMLRVPQMATGGKYAVSWDHFFTTLGNYYTSMRNDFNTNIGMSGETIYRTRSTPKAITQREAEHLVAVMGIIQAVAEHDEVSRIMICDHPNWQTPQVLLGLVACATPLFLKAEILHTLAALAKSKETARVIWFHLEASQIIPTVPVSRSYAQCSLLEEMEQIESRSEQYNLTRGILQLLYTLMTTNLPKSLGAGQRTPAYEGYLKSMINSVLLKFYNRAYKVPSEKWQVGAQCLKLLYYLLATYRPSAMDFLETVDEPPYPGFHVMMQLQLKSEMLQLLLCIVEEVRERLDNYNRFQGKKLLEECSLYALLILEAALAKQNAFFEAHSAANCPILLSGLNRMLLDLNPRSRKPDHVLNIIKYVTYNSWLPRHALAAIKILASVTQLPNVSTQILSMYGQGSNEKLEIRQGFVECLEMEVCVGKHDDDLLDQLALNNHVPYLGFGDDLDNEREMSGEREYSTIESQLELQAGDGALESKPASIELQLKEAIIKLFEMNLSQQLPNFVYFLLGVDVLREFMANGRQHLAIEMQSSCVNSVVLLLEKYMDKQRHSDKYCEHTARIVERIYHLFHGLCANRRTAETILRYFRLTCSDFLLRHLRSLPFRQHREDHVLHAMGHLLNCVSIDVKLAAKHGQMTRFNQMCDILLIGNGMERSSHGLSMELGHSLISQPSSSFLAMDVLPAGGSISAVAHGAGSASLGAPNTGVKSLKPSLLQETSQGLHVTRLLDILVLEAGTLSQPQLEFFDGHLITQLLRDCEASAEAGANSRANLINVRKLYYILTDELYMVQSIIASGQRKAISTEIMLLLNHAVNINRVRTQRCATLAFMAAWGQLVQVLFSNMPDAVLPATQRRQHIIDIVEKVLIKVQPIQPIIEISVQVSETVLLLLANLRYCCYQAEDQSPEDLAAEDSLSNGNGNAIGNDSQVNALCLGQRAIGTGSDGGSGGGRDIGSGGNSSNLRFILKNLVQWIMISEVKSQKLRINLYSSLLNCLRIAKRLRTDEHLEYQETLISRQESARTYNKEQRRDDRLRLKAMAAEVIGTFGEKLIDTICHDAVTGHDVCRMLALACLDMISELQAVSTLCDFVASRGYLKHILESLDQSSTALCGILQPVPDHLRPLYVYESRMAFLTRMANSNVGARLLLTEQALGVLSNMKVYDQQPDLKSSELNRNEPQTFLPSIDDRFRSILLPALSLCDAIVNSLGPRNNSAAVQVLNFLFAHIDMVEAMLRSATPYMDLGHLQQVAVISNLFARASTHELTALEDSVQLDLRNRLGRVQQLMIVVFGRFCVSEPTIRRMLQQDEEQQTNPTDDSKRLRVKYFLDIAANVSLYCRNVVTSHSKDSMTSKYLLTTVINDVTLLTGKMSSKKLTTIMHTILNQLKGSIGYYLSQKSIADNLLQQRASLPNISFGPNGKQSYIELSQRYNEKRSELRQAVFIAEQNLYLLWIHLDFYLRNTIDYANENRNAINESNMDDDNDMSVLNASQEEIVQLKQLLISTFNETFCTQLINASEDYSIKCKGFNGSLLRRIKALVQFAPITANDVNSSFDS

Target ORF information:

RefSeq Version	NM_167687.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 205kD, transcript variant B (Nup205), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167687.3

ORF Insert Sequence:

ATGGATGGTG AGTGCCTGGC CAACGAGCAG ATGTGCGTGG GTCGCCAAAT CACTAATGCC 
ACCCATGCGT CCACAGCTGT AATCGAGGAC ATGTGGACGC CGTACAAGCA TTTGTACAAC 
ACTTTTCAGA CCGCGGTGTC CAATCGCAGT GGCTTCACCT CGGATCTGGA GCAGTGCCTC 
AAGAAGTACA AGCACAACTT CAGCAACTTG CTGCGCAATC CGCCGAGAAG CGAGAAGAGC 
CGCAATCTCC TACGAAATGC CCTCAACGAA GGCGTCCCGA TGCAGGGGCA TTCGCGAAAG 
ACAAAGATGT CACAGGACCT GGCCGACGAG GCGGTCATTC TGTCCGACAT GTTCGATCTG 
GACGAGGGTT TCGCCGTGGA GCTGCTGTGC ACCGCACAGC GCCAGCAGAA GCATCATCCC 
GGCCTGTCCA GAGGCCTTGT CGCGGTGCTG CTCTACTACG ATGGACGAAA GGCCATCAGC 
TGCACGCTTC GCGACATGTT CCAGGTGGTT AGCGGCGTCT CGTGGAACAC CGAACTGCCC 
AAGGAGATCA CCGGTCTGGT CACTAACTAT GCCGAGAGCC TTGTCGACGG CTCTGGGATA 
CTGGGACGCC TGCTGCAGCT GCTCGAGGAG ATGGATGTCG ACAAGGAGTG CGCTATGCTG 
ACCACAAACC GCGCGTTTGG CTCCAAGAAG CATCAGAACC AGGTGCTCGG CCTATACGAG 
GACATTCAAC AGGCATTGGC AATGGCCCTG TTCAATTGGT CGGCACAACG CGGCCTGCCG 
CGACACATTG CCATTCGGCT GATGCACCAA TTGGCCAACC GAAAGAACCA CGACGCGGGC 
GGCAACATGG ACGACGTGAC CCTGATCATG CTGATGGCTC TGCTGTATGC GTACGATACC 
TCCATGCTGC TGGTCACCGA GGAACCCAAT GAGCACACAA CCCGACTTCC GATCTTCAGC 
GATCGCGAGT TCGCCGAGTG CTTCCTGGAG GAGCTGTACG CGCAGAGCAG CTGGCAAGCA 
CCGCGCCTGA ATGCCATCAT TGCCTACAGC TTCGGACTAA CGCTGGCCAG CCTGCGGCAT 
GCACCTTTGC AGCTTCAGGC CACCGCCATC TCGATCATCA ATCGCGATGA GATGCTCATC 
GACGAGGCAC TGGGAGCGCA GGTATTCGTC TTCTTCCACA GCTTGCTGCT CGAGAAGGAT 
CTGGTCTATA GCACGCAGTT CTTCTACCGC CGCGTCCATC TGCTCATCAC GGACTTCATC 
GACTTCATGA ACTCCAAGGT GTCGGAGCTG CGCGGCCGAG CAGACGAGTC GTCACACACG 
ATCATCAGCT TCCTAAACGA GGGTCTGGAA CCGCCGCCCA ACTTGGACTC CAACTTTGAG 
CTGCTCATGC TGTGCGTGGC CAAGATGCAC GGCGATCCGC GCGTCACCAT CAGGCTGTGC 
AACGAGTACT GGGGCCCCGG CGATCCCAAC GGCTCTACGG CATTCAAGAA CACCTCGCGC 
TCGGTGTCGC TGTTCAAGTT CATTAGCTTG GCCAGCGACC TGCTGCCACA GACGCTGTTC 
AAGGCCTACC TCAAGATGAT ATCCGGACTT ACACGGACCG ATTTCTCGGC TCGATGCGCC 
TTCAACATGC TTAGGGTGCC CCAGATGGCC ACCGGCGGCA AGTATGCGGT TAGCTGGGAT 
CACTTCTTCA CCACCCTGGG CAACTACTAT ACTTCGATGC GCAACGACTT TAACACCAAC 
ATCGGCATGA GCGGCGAGAC CATTTACCGC ACCCGATCGA CACCAAAGGC CATTACGCAG 
CGAGAGGCGG AGCATCTGGT GGCCGTAATG GGCATCATAC AGGCGGTGGC GGAACACGAC 
GAGGTCTCGC GCATCATGAT CTGTGACCAT CCCAATTGGC AGACGCCGCA GGTGCTCCTG 
GGCCTGGTTG CGTGCGCCAC GCCGCTTTTT TTGAAGGCGG AGATTCTGCA CACGCTGGCT 
GCCCTGGCCA AATCGAAGGA GACGGCGCGC GTCATCTGGT TCCACCTGGA GGCCTCGCAG 
ATTATACCCA CTGTGCCCGT GTCTAGAAGC TATGCCCAGT GCAGTTTGTT GGAGGAGATG 
GAACAAATCG AGAGCCGTTC GGAGCAGTAT AACCTGACGC GCGGCATCCT CCAATTGCTC 
TACACACTGA TGACCACCAA TCTGCCGAAG AGTTTGGGCG CCGGGCAACG GACACCGGCC 
TACGAAGGCT ATCTAAAGTC CATGATAAAT TCGGTGTTGC TCAAGTTTTA CAATCGCGCG 
TACAAGGTTC CCTCCGAGAA GTGGCAAGTT GGCGCCCAGT GCCTGAAACT GCTCTACTAC 
CTACTGGCCA CCTATCGGCC GTCCGCAATG GATTTTCTGG AGACAGTCGA TGAACCTCCC 
TATCCGGGCT TTCACGTTAT GATGCAGCTG CAGCTGAAGT CCGAAATGTT GCAGCTGCTG 
CTGTGCATTG TGGAGGAGGT GCGTGAGCGG CTGGACAACT ACAATCGCTT TCAGGGCAAG 
AAGCTACTCG AGGAGTGCTC ATTGTACGCA CTGCTGATCC TGGAAGCCGC ATTGGCGAAA 
CAGAACGCCT TCTTTGAGGC CCACTCGGCG GCCAATTGCC CCATTTTGCT GTCGGGCCTG 
AATCGCATGC TCCTGGATCT GAATCCACGC AGTCGCAAGC CCGATCACGT GCTGAATATC 
ATTAAGTATG TAACCTATAA TAGCTGGCTG CCGCGGCACG CCCTGGCCGC CATTAAGATT 
CTGGCTTCGG TCACCCAGCT GCCGAATGTC TCCACCCAGA TTCTCAGCAT GTACGGTCAG 
GGCAGCAATG AGAAGCTCGA GATTCGTCAG GGTTTCGTCG AGTGCCTGGA AATGGAGGTG 
TGCGTGGGCA AGCACGACGA CGATTTGTTG GACCAACTGG CCCTCAACAA TCACGTTCCG 
TATCTGGGAT TCGGCGATGA TCTGGACAAT GAGCGAGAGA TGAGCGGGGA ACGCGAATAT 
TCAACTATTG AATCGCAACT GGAGCTGCAG GCGGGGGATG GCGCGCTGGA GAGCAAACCG 
GCTAGCATTG AGCTGCAACT GAAAGAGGCC ATTATCAAGC TGTTCGAGAT GAATCTTAGC 
CAGCAGTTGC CCAACTTTGT GTACTTCCTG CTCGGCGTGG ATGTGCTGCG CGAATTTATG 
GCCAACGGAC GGCAGCACCT GGCCATCGAG ATGCAAAGCT CGTGCGTGAA CTCCGTAGTG 
CTGTTGCTGG AAAAGTACAT GGATAAACAG CGTCACAGCG ATAAGTACTG CGAGCACACG 
GCGAGAATCG TGGAGCGCAT TTACCATCTG TTCCATGGAC TCTGCGCCAA TCGCCGCACC 
GCGGAGACGA TACTGCGCTA CTTCCGGCTA ACGTGCAGCG ACTTCCTGCT GCGTCACCTA 
CGCTCGCTGC CCTTCCGGCA GCATCGCGAG GATCATGTGC TGCACGCCAT GGGCCACCTG 
CTCAACTGCG TCTCTATCGA TGTCAAACTG GCGGCCAAAC ATGGCCAGAT GACGCGCTTC 
AACCAGATGT GCGACATTTT GCTAATTGGA AACGGAATGG AGCGTTCATC CCATGGCCTG 
TCGATGGAAT TGGGCCACAG CCTGATTTCA CAGCCGTCGT CGTCTTTCCT CGCCATGGAT 
GTGCTACCGG CCGGTGGATC GATAAGTGCC GTGGCACATG GGGCTGGTTC GGCGTCTCTC 
GGAGCTCCAA ACACTGGGGT CAAATCACTT AAGCCATCGC TGCTGCAGGA GACATCGCAG 
GGACTGCATG TCACCCGACT GCTAGATATT CTCGTCCTGG AGGCGGGCAC GTTGAGCCAG 
CCGCAGCTGG AGTTCTTCGA CGGGCATCTG ATCACTCAAC TGCTGCGCGA CTGCGAGGCA 
TCCGCCGAAG CTGGCGCCAA TTCCAGGGCC AACTTGATCA ACGTGCGCAA ACTGTACTAC 
ATTCTCACCG ACGAGCTGTA CATGGTGCAG AGCATCATTG CCAGCGGCCA GCGGAAGGCC 
ATTTCCACGG AAATCATGCT GCTGCTCAAT CACGCCGTCA ACATAAATCG CGTGCGGACA 
CAGCGCTGCG CCACGCTGGC CTTCATGGCA GCATGGGGTC AACTGGTGCA GGTGCTCTTT 
AGCAACATGC CCGATGCCGT GCTTCCGGCG ACGCAGCGTC GCCAGCACAT CATCGATATC 
GTCGAGAAGG TACTCATCAA GGTGCAGCCC ATACAGCCCA TCATCGAGAT ATCCGTACAG 
GTGAGCGAGA CGGTGCTGCT GCTGCTTGCC AATTTGCGCT ACTGCTGCTA CCAGGCCGAG 
GACCAGAGCC CCGAGGATCT GGCCGCCGAG GATTCGCTCA GCAATGGCAA TGGCAACGCC 
ATCGGCAACG ATTCGCAGGT GAACGCATTG TGTCTGGGCC AGAGGGCAAT CGGAACTGGT 
TCGGATGGCG GCAGTGGAGG TGGACGGGAT ATCGGTTCCG GTGGCAACAG CAGCAATCTG 
CGATTCATTC TCAAGAACCT CGTCCAGTGG ATCATGATCA GTGAGGTGAA GTCGCAGAAG 
CTGCGCATCA ATCTGTACAG TTCGCTGCTG AATTGCCTGC GGATCGCGAA GCGACTGCGC 
ACCGACGAGC ATCTGGAGTA CCAGGAGACA TTGATTTCGC GCCAGGAGAG TGCACGCACG 
TACAACAAGG AGCAGCGGCG CGACGATCGC CTGCGGCTGA AGGCCATGGC GGCGGAAGTG 
ATTGGCACGT TCGGCGAGAA GCTGATCGAC ACCATCTGCC ACGATGCGGT GACCGGCCAC 
GATGTGTGCC GCATGCTGGC GCTGGCCTGC CTGGACATGA TCTCCGAGCT GCAGGCGGTG 
AGCACGCTAT GCGACTTTGT GGCATCGCGT GGCTACCTCA AGCACATACT GGAAAGCCTT 
GATCAGTCCA GTACGGCGCT TTGCGGGATT CTGCAGCCCG TGCCGGATCA CCTGAGGCCG 
CTCTACGTCT ACGAGTCGCG CATGGCCTTC CTCACGCGCA TGGCGAACAG CAATGTGGGA 
GCCCGGCTCC TCCTCACCGA GCAGGCGCTG GGCGTGCTTT CGAATATGAA GGTCTATGAC 
CAGCAGCCCG ACCTGAAGTC CAGCGAACTG AATCGTAACG AGCCGCAGAC CTTCCTCCCG 
TCCATCGATG ACCGCTTCCG GTCGATCCTC CTGCCGGCGC TGAGCCTCTG CGATGCCATT 
GTCAACTCGC TGGGTCCGCG CAACAACTCC GCCGCCGTGC AGGTGCTTAA CTTTCTGTTC 
GCCCACATCG ACATGGTCGA GGCCATGTTG CGCTCGGCCA CGCCGTATAT GGACCTCGGC 
CACCTGCAGC AGGTGGCGGT CATTAGCAAT CTGTTTGCCC GCGCCTCCAC GCACGAGTTG 
ACCGCGCTGG AGGATAGCGT GCAGCTGGAT TTGCGAAATC GCCTGGGCCG CGTGCAGCAG 
CTGATGATCG TGGTGTTCGG ACGCTTTTGC GTAAGCGAGC CCACCATTCG TCGCATGCTG 
CAGCAGGATG AGGAGCAGCA GACCAACCCC ACCGATGACT CGAAGAGGCT ACGTGTCAAG 
TATTTTCTGG ATATAGCAGC CAACGTGTCG CTCTACTGCC GCAACGTGGT CACCAGCCAT 
TCGAAGGACA GCATGACCTC CAAATACTTG CTGACCACCG TGATCAATGA TGTAACGCTG 
CTCACTGGCA AAATGTCCAG CAAAAAGCTG ACGACCATCA TGCACACCAT CCTCAATCAG 
CTGAAGGGCT CCATCGGCTA CTACCTTTCG CAGAAGTCGA TCGCCGACAA TCTCCTCCAG 
CAACGCGCCT CCCTGCCCAA CATCAGCTTT GGGCCCAACG GTAAACAAAG CTACATAGAA 
CTGAGCCAGC GATACAACGA GAAGCGCAGC GAACTGAGGC AGGCCGTCTT CATTGCCGAG 
CAGAATCTGT ATCTGCTGTG GATCCATCTG GACTTCTATC TGCGCAACAC CATCGACTAC 
GCCAACGAAA ACAGGAACGC CATCAACGAG AGCAACATGG ACGACGACAA TGACATGTCC 
GTGCTGAACG CCTCGCAGGA AGAGATCGTG CAGCTCAAGC AGCTGCTCAT CTCCACATTC 
AACGAGACAT TCTGCACGCA GCTAATCAAC GCCAGCGAGG ATTACTCGAT TAAGTGCAAA 
GGCTTCAATG GCTCGCTGCT GCGACGCATT AAGGCGCTCG TCCAGTTCGC CCCCATCACT 
GCCAACGATG TCAATTCCAG CTTCGATTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup205 ( NM_134518.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608362.2 Drosophila melanogaster nucleoporin 205kD, transcript variant A (Nup205), mRNA.

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CloneID	OFa02192
Clone ID Related Accession (Same CDS sequence)	NM_134518.4
Accession Version	NM_134518.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 205kD, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_134518.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGCTG TAATCGAGGA CATGTGGACG CCGTACAAGC ATTTGTACAA CACTTTTCAG 
ACCGCGGTGT CCAATCGCAG TGGCTTCACC TCGGATCTGG AGCAGTGCCT CAAGAAGTAC 
AAGCACAACT TCAGCAACTT GCTGCGCAAT CCGCCGAGAA GCGAGAAGAG CCGCAATCTC 
CTACGAAATG CCCTCAACGA AGGCGTCCCG ATGCAGGGGC ATTCGCGAAA GACAAAGATG 
TCACAGGACC TGGCCGACGA GGCGGTCATT CTGTCCGACA TGTTCGATCT GGACGAGGGT 
TTCGCCGTGG AGCTGCTGTG CACCGCACAG CGCCAGCAGA AGCATCATCC CGGCCTGTCC 
AGAGGCCTTG TCGCGGTGCT GCTCTACTAC GATGGACGAA AGGCCATCAG CTGCACGCTT 
CGCGACATGT TCCAGGTGGT TAGCGGCGTC TCGTGGAACA CCGAACTGCC CAAGGAGATC 
ACCGGTCTGG TCACTAACTA TGCCGAGAGC CTTGTCGACG GCTCTGGGAT ACTGGGACGC 
CTGCTGCAGC TGCTCGAGGA GATGGATGTC GACAAGGAGT GCGCTATGCT GACCACAAAC 
CGCGCGTTTG GCTCCAAGAA GCATCAGAAC CAGGTGCTCG GCCTATACGA GGACATTCAA 
CAGGCATTGG CAATGGCCCT GTTCAATTGG TCGGCACAAC GCGGCCTGCC GCGACACATT 
GCCATTCGGC TGATGCACCA ATTGGCCAAC CGAAAGAACC ACGACGCGGG CGGCAACATG 
GACGACGTGA CCCTGATCAT GCTGATGGCT CTGCTGTATG CGTACGATAC CTCCATGCTG 
CTGGTCACCG AGGAACCCAA TGAGCACACA ACCCGACTTC CGATCTTCAG CGATCGCGAG 
TTCGCCGAGT GCTTCCTGGA GGAGCTGTAC GCGCAGAGCA GCTGGCAAGC ACCGCGCCTG 
AATGCCATCA TTGCCTACAG CTTCGGACTA ACGCTGGCCA GCCTGCGGCA TGCACCTTTG 
CAGCTTCAGG CCACCGCCAT CTCGATCATC AATCGCGATG AGATGCTCAT CGACGAGGCA 
CTGGGAGCGC AGGTATTCGT CTTCTTCCAC AGCTTGCTGC TCGAGAAGGA TCTGGTCTAT 
AGCACGCAGT TCTTCTACCG CCGCGTCCAT CTGCTCATCA CGGACTTCAT CGACTTCATG 
AACTCCAAGG TGTCGGAGCT GCGCGGCCGA GCAGACGAGT CGTCACACAC GATCATCAGC 
TTCCTAAACG AGGGTCTGGA ACCGCCGCCC AACTTGGACT CCAACTTTGA GCTGCTCATG 
CTGTGCGTGG CCAAGATGCA CGGCGATCCG CGCGTCACCA TCAGGCTGTG CAACGAGTAC 
TGGGGCCCCG GCGATCCCAA CGGCTCTACG GCATTCAAGA ACACCTCGCG CTCGGTGTCG 
CTGTTCAAGT TCATTAGCTT GGCCAGCGAC CTGCTGCCAC AGACGCTGTT CAAGGCCTAC 
CTCAAGATGA TATCCGGACT TACACGGACC GATTTCTCGG CTCGATGCGC CTTCAACATG 
CTTAGGGTGC CCCAGATGGC CACCGGCGGC AAGTATGCGG TTAGCTGGGA TCACTTCTTC 
ACCACCCTGG GCAACTACTA TACTTCGATG CGCAACGACT TTAACACCAA CATCGGCATG 
AGCGGCGAGA CCATTTACCG CACCCGATCG ACACCAAAGG CCATTACGCA GCGAGAGGCG 
GAGCATCTGG TGGCCGTAAT GGGCATCATA CAGGCGGTGG CGGAACACGA CGAGGTCTCG 
CGCATCATGA TCTGTGACCA TCCCAATTGG CAGACGCCGC AGGTGCTCCT GGGCCTGGTT 
GCGTGCGCCA CGCCGCTTTT TTTGAAGGCG GAGATTCTGC ACACGCTGGC TGCCCTGGCC 
AAATCGAAGG AGACGGCGCG CGTCATCTGG TTCCACCTGG AGGCCTCGCA GATTATACCC 
ACTGTGCCCG TGTCTAGAAG CTATGCCCAG TGCAGTTTGT TGGAGGAGAT GGAACAAATC 
GAGAGCCGTT CGGAGCAGTA TAACCTGACG CGCGGCATCC TCCAATTGCT CTACACACTG 
ATGACCACCA ATCTGCCGAA GAGTTTGGGC GCCGGGCAAC GGACACCGGC CTACGAAGGC 
TATCTAAAGT CCATGATAAA TTCGGTGTTG CTCAAGTTTT ACAATCGCGC GTACAAGGTT 
CCCTCCGAGA AGTGGCAAGT TGGCGCCCAG TGCCTGAAAC TGCTCTACTA CCTACTGGCC 
ACCTATCGGC CGTCCGCAAT GGATTTTCTG GAGACAGTCG ATGAACCTCC CTATCCGGGC 
TTTCACGTTA TGATGCAGCT GCAGCTGAAG TCCGAAATGT TGCAGCTGCT GCTGTGCATT 
GTGGAGGAGG TGCGTGAGCG GCTGGACAAC TACAATCGCT TTCAGGGCAA GAAGCTACTC 
GAGGAGTGCT CATTGTACGC ACTGCTGATC CTGGAAGCCG CATTGGCGAA ACAGAACGCC 
TTCTTTGAGG CCCACTCGGC GGCCAATTGC CCCATTTTGC TGTCGGGCCT GAATCGCATG 
CTCCTGGATC TGAATCCACG CAGTCGCAAG CCCGATCACG TGCTGAATAT CATTAAGTAT 
GTAACCTATA ATAGCTGGCT GCCGCGGCAC GCCCTGGCCG CCATTAAGAT TCTGGCTTCG 
GTCACCCAGC TGCCGAATGT CTCCACCCAG ATTCTCAGCA TGTACGGTCA GGGCAGCAAT 
GAGAAGCTCG AGATTCGTCA GGGTTTCGTC GAGTGCCTGG AAATGGAGGT GTGCGTGGGC 
AAGCACGACG ACGATTTGTT GGACCAACTG GCCCTCAACA ATCACGTTCC GTATCTGGGA 
TTCGGCGATG ATCTGGACAA TGAGCGAGAG ATGAGCGGGG AACGCGAATA TTCAACTATT 
GAATCGCAAC TGGAGCTGCA GGCGGGGGAT GGCGCGCTGG AGAGCAAACC GGCTAGCATT 
GAGCTGCAAC TGAAAGAGGC CATTATCAAG CTGTTCGAGA TGAATCTTAG CCAGCAGTTG 
CCCAACTTTG TGTACTTCCT GCTCGGCGTG GATGTGCTGC GCGAATTTAT GGCCAACGGA 
CGGCAGCACC TGGCCATCGA GATGCAAAGC TCGTGCGTGA ACTCCGTAGT GCTGTTGCTG 
GAAAAGTACA TGGATAAACA GCGTCACAGC GATAAGTACT GCGAGCACAC GGCGAGAATC 
GTGGAGCGCA TTTACCATCT GTTCCATGGA CTCTGCGCCA ATCGCCGCAC CGCGGAGACG 
ATACTGCGCT ACTTCCGGCT AACGTGCAGC GACTTCCTGC TGCGTCACCT ACGCTCGCTG 
CCCTTCCGGC AGCATCGCGA GGATCATGTG CTGCACGCCA TGGGCCACCT GCTCAACTGC 
GTCTCTATCG ATGTCAAACT GGCGGCCAAA CATGGCCAGA TGACGCGCTT CAACCAGATG 
TGCGACATTT TGCTAATTGG AAACGGAATG GAGCGTTCAT CCCATGGCCT GTCGATGGAA 
TTGGGCCACA GCCTGATTTC ACAGCCGTCG TCGTCTTTCC TCGCCATGGA TGTGCTACCG 
GCCGGTGGAT CGATAAGTGC CGTGGCACAT GGGGCTGGTT CGGCGTCTCT CGGAGCTCCA 
AACACTGGGG TCAAATCACT TAAGCCATCG CTGCTGCAGG AGACATCGCA GGGACTGCAT 
GTCACCCGAC TGCTAGATAT TCTCGTCCTG GAGGCGGGCA CGTTGAGCCA GCCGCAGCTG 
GAGTTCTTCG ACGGGCATCT GATCACTCAA CTGCTGCGCG ACTGCGAGGC ATCCGCCGAA 
GCTGGCGCCA ATTCCAGGGC CAACTTGATC AACGTGCGCA AACTGTACTA CATTCTCACC 
GACGAGCTGT ACATGGTGCA GAGCATCATT GCCAGCGGCC AGCGGAAGGC CATTTCCACG 
GAAATCATGC TGCTGCTCAA TCACGCCGTC AACATAAATC GCGTGCGGAC ACAGCGCTGC 
GCCACGCTGG CCTTCATGGC AGCATGGGGT CAACTGGTGC AGGTGCTCTT TAGCAACATG 
CCCGATGCCG TGCTTCCGGC GACGCAGCGT CGCCAGCACA TCATCGATAT CGTCGAGAAG 
GTACTCATCA AGGTGCAGCC CATACAGCCC ATCATCGAGA TATCCGTACA GGTGAGCGAG 
ACGGTGCTGC TGCTGCTTGC CAATTTGCGC TACTGCTGCT ACCAGGCCGA GGACCAGAGC 
CCCGAGGATC TGGCCGCCGA GGATTCGCTC AGCAATGGCA ATGGCAACGC CATCGGCAAC 
GATTCGCAGG TGAACGCATT GTGTCTGGGC CAGAGGGCAA TCGGAACTGG TTCGGATGGC 
GGCAGTGGAG GTGGACGGGA TATCGGTTCC GGTGGCAACA GCAGCAATCT GCGATTCATT 
CTCAAGAACC TCGTCCAGTG GATCATGATC AGTGAGGTGA AGTCGCAGAA GCTGCGCATC 
AATCTGTACA GTTCGCTGCT GAATTGCCTG CGGATCGCGA AGCGACTGCG CACCGACGAG 
CATCTGGAGT ACCAGGAGAC ATTGATTTCG CGCCAGGAGA GTGCACGCAC GTACAACAAG 
GAGCAGCGGC GCGACGATCG CCTGCGGCTG AAGGCCATGG CGGCGGAAGT GATTGGCACG 
TTCGGCGAGA AGCTGATCGA CACCATCTGC CACGATGCGG TGACCGGCCA CGATGTGTGC 
CGCATGCTGG CGCTGGCCTG CCTGGACATG ATCTCCGAGC TGCAGGCGGT GAGCACGCTA 
TGCGACTTTG TGGCATCGCG TGGCTACCTC AAGCACATAC TGGAAAGCCT TGATCAGTCC 
AGTACGGCGC TTTGCGGGAT TCTGCAGCCC GTGCCGGATC ACCTGAGGCC GCTCTACGTC 
TACGAGTCGC GCATGGCCTT CCTCACGCGC ATGGCGAACA GCAATGTGGG AGCCCGGCTC 
CTCCTCACCG AGCAGGCGCT GGGCGTGCTT TCGAATATGA AGGTCTATGA CCAGCAGCCC 
GACCTGAAGT CCAGCGAACT GAATCGTAAC GAGCCGCAGA CCTTCCTCCC GTCCATCGAT 
GACCGCTTCC GGTCGATCCT CCTGCCGGCG CTGAGCCTCT GCGATGCCAT TGTCAACTCG 
CTGGGTCCGC GCAACAACTC CGCCGCCGTG CAGGTGCTTA ACTTTCTGTT CGCCCACATC 
GACATGGTCG AGGCCATGTT GCGCTCGGCC ACGCCGTATA TGGACCTCGG CCACCTGCAG 
CAGGTGGCGG TCATTAGCAA TCTGTTTGCC CGCGCCTCCA CGCACGAGTT GACCGCGCTG 
GAGGATAGCG TGCAGCTGGA TTTGCGAAAT CGCCTGGGCC GCGTGCAGCA GCTGATGATC 
GTGGTGTTCG GACGCTTTTG CGTAAGCGAG CCCACCATTC GTCGCATGCT GCAGCAGGAT 
GAGGAGCAGC AGACCAACCC CACCGATGAC TCGAAGAGGC TACGTGTCAA GTATTTTCTG 
GATATAGCAG CCAACGTGTC GCTCTACTGC CGCAACGTGG TCACCAGCCA TTCGAAGGAC 
AGCATGACCT CCAAATACTT GCTGACCACC GTGATCAATG ATGTAACGCT GCTCACTGGC 
AAAATGTCCA GCAAAAAGCT GACGACCATC ATGCACACCA TCCTCAATCA GCTGAAGGGC 
TCCATCGGCT ACTACCTTTC GCAGAAGTCG ATCGCCGACA ATCTCCTCCA GCAACGCGCC 
TCCCTGCCCA ACATCAGCTT TGGGCCCAAC GGTAAACAAA GCTACATAGA ACTGAGCCAG 
CGATACAACG AGAAGCGCAG CGAACTGAGG CAGGCCGTCT TCATTGCCGA GCAGAATCTG 
TATCTGCTGT GGATCCATCT GGACTTCTAT CTGCGCAACA CCATCGACTA CGCCAACGAA 
AACAGGAACG CCATCAACGA GAGCAACATG GACGACGACA ATGACATGTC CGTGCTGAAC 
GCCTCGCAGG AAGAGATCGT GCAGCTCAAG CAGCTGCTCA TCTCCACATT CAACGAGACA 
TTCTGCACGC AGCTAATCAA CGCCAGCGAG GATTACTCGA TTAAGTGCAA AGGCTTCAAT 
GGCTCGCTGC TGCGACGCAT TAAGGCGCTC GTCCAGTTCG CCCCCATCAC TGCCAACGAT 
GTCAATTCCA GCTTCGATTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608362.2
CDS	135..6338
Translation	MDAVIEDMWTPYKHLYNTFQTAVSNRSGFTSDLEQCLKKYKHNFSNLLRNPPRSEKSRNLLRNALNEGVPMQGHSRKTKMSQDLADEAVILSDMFDLDEGFAVELLCTAQRQQKHHPGLSRGLVAVLLYYDGRKAISCTLRDMFQVVSGVSWNTELPKEITGLVTNYAESLVDGSGILGRLLQLLEEMDVDKECAMLTTNRAFGSKKHQNQVLGLYEDIQQALAMALFNWSAQRGLPRHIAIRLMHQLANRKNHDAGGNMDDVTLIMLMALLYAYDTSMLLVTEEPNEHTTRLPIFSDREFAECFLEELYAQSSWQAPRLNAIIAYSFGLTLASLRHAPLQLQATAISIINRDEMLIDEALGAQVFVFFHSLLLEKDLVYSTQFFYRRVHLLITDFIDFMNSKVSELRGRADESSHTIISFLNEGLEPPPNLDSNFELLMLCVAKMHGDPRVTIRLCNEYWGPGDPNGSTAFKNTSRSVSLFKFISLASDLLPQTLFKAYLKMISGLTRTDFSARCAFNMLRVPQMATGGKYAVSWDHFFTTLGNYYTSMRNDFNTNIGMSGETIYRTRSTPKAITQREAEHLVAVMGIIQAVAEHDEVSRIMICDHPNWQTPQVLLGLVACATPLFLKAEILHTLAALAKSKETARVIWFHLEASQIIPTVPVSRSYAQCSLLEEMEQIESRSEQYNLTRGILQLLYTLMTTNLPKSLGAGQRTPAYEGYLKSMINSVLLKFYNRAYKVPSEKWQVGAQCLKLLYYLLATYRPSAMDFLETVDEPPYPGFHVMMQLQLKSEMLQLLLCIVEEVRERLDNYNRFQGKKLLEECSLYALLILEAALAKQNAFFEAHSAANCPILLSGLNRMLLDLNPRSRKPDHVLNIIKYVTYNSWLPRHALAAIKILASVTQLPNVSTQILSMYGQGSNEKLEIRQGFVECLEMEVCVGKHDDDLLDQLALNNHVPYLGFGDDLDNEREMSGEREYSTIESQLELQAGDGALESKPASIELQLKEAIIKLFEMNLSQQLPNFVYFLLGVDVLREFMANGRQHLAIEMQSSCVNSVVLLLEKYMDKQRHSDKYCEHTARIVERIYHLFHGLCANRRTAETILRYFRLTCSDFLLRHLRSLPFRQHREDHVLHAMGHLLNCVSIDVKLAAKHGQMTRFNQMCDILLIGNGMERSSHGLSMELGHSLISQPSSSFLAMDVLPAGGSISAVAHGAGSASLGAPNTGVKSLKPSLLQETSQGLHVTRLLDILVLEAGTLSQPQLEFFDGHLITQLLRDCEASAEAGANSRANLINVRKLYYILTDELYMVQSIIASGQRKAISTEIMLLLNHAVNINRVRTQRCATLAFMAAWGQLVQVLFSNMPDAVLPATQRRQHIIDIVEKVLIKVQPIQPIIEISVQVSETVLLLLANLRYCCYQAEDQSPEDLAAEDSLSNGNGNAIGNDSQVNALCLGQRAIGTGSDGGSGGGRDIGSGGNSSNLRFILKNLVQWIMISEVKSQKLRINLYSSLLNCLRIAKRLRTDEHLEYQETLISRQESARTYNKEQRRDDRLRLKAMAAEVIGTFGEKLIDTICHDAVTGHDVCRMLALACLDMISELQAVSTLCDFVASRGYLKHILESLDQSSTALCGILQPVPDHLRPLYVYESRMAFLTRMANSNVGARLLLTEQALGVLSNMKVYDQQPDLKSSELNRNEPQTFLPSIDDRFRSILLPALSLCDAIVNSLGPRNNSAAVQVLNFLFAHIDMVEAMLRSATPYMDLGHLQQVAVISNLFARASTHELTALEDSVQLDLRNRLGRVQQLMIVVFGRFCVSEPTIRRMLQQDEEQQTNPTDDSKRLRVKYFLDIAANVSLYCRNVVTSHSKDSMTSKYLLTTVINDVTLLTGKMSSKKLTTIMHTILNQLKGSIGYYLSQKSIADNLLQQRASLPNISFGPNGKQSYIELSQRYNEKRSELRQAVFIAEQNLYLLWIHLDFYLRNTIDYANENRNAINESNMDDDNDMSVLNASQEEIVQLKQLLISTFNETFCTQLINASEDYSIKCKGFNGSLLRRIKALVQFAPITANDVNSSFDS

Target ORF information:

RefSeq Version	NM_134518.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 205kD, transcript variant A (Nup205), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134518.4

ORF Insert Sequence:

ATGGATGCTG TAATCGAGGA CATGTGGACG CCGTACAAGC ATTTGTACAA CACTTTTCAG 
ACCGCGGTGT CCAATCGCAG TGGCTTCACC TCGGATCTGG AGCAGTGCCT CAAGAAGTAC 
AAGCACAACT TCAGCAACTT GCTGCGCAAT CCGCCGAGAA GCGAGAAGAG CCGCAATCTC 
CTACGAAATG CCCTCAACGA AGGCGTCCCG ATGCAGGGGC ATTCGCGAAA GACAAAGATG 
TCACAGGACC TGGCCGACGA GGCGGTCATT CTGTCCGACA TGTTCGATCT GGACGAGGGT 
TTCGCCGTGG AGCTGCTGTG CACCGCACAG CGCCAGCAGA AGCATCATCC CGGCCTGTCC 
AGAGGCCTTG TCGCGGTGCT GCTCTACTAC GATGGACGAA AGGCCATCAG CTGCACGCTT 
CGCGACATGT TCCAGGTGGT TAGCGGCGTC TCGTGGAACA CCGAACTGCC CAAGGAGATC 
ACCGGTCTGG TCACTAACTA TGCCGAGAGC CTTGTCGACG GCTCTGGGAT ACTGGGACGC 
CTGCTGCAGC TGCTCGAGGA GATGGATGTC GACAAGGAGT GCGCTATGCT GACCACAAAC 
CGCGCGTTTG GCTCCAAGAA GCATCAGAAC CAGGTGCTCG GCCTATACGA GGACATTCAA 
CAGGCATTGG CAATGGCCCT GTTCAATTGG TCGGCACAAC GCGGCCTGCC GCGACACATT 
GCCATTCGGC TGATGCACCA ATTGGCCAAC CGAAAGAACC ACGACGCGGG CGGCAACATG 
GACGACGTGA CCCTGATCAT GCTGATGGCT CTGCTGTATG CGTACGATAC CTCCATGCTG 
CTGGTCACCG AGGAACCCAA TGAGCACACA ACCCGACTTC CGATCTTCAG CGATCGCGAG 
TTCGCCGAGT GCTTCCTGGA GGAGCTGTAC GCGCAGAGCA GCTGGCAAGC ACCGCGCCTG 
AATGCCATCA TTGCCTACAG CTTCGGACTA ACGCTGGCCA GCCTGCGGCA TGCACCTTTG 
CAGCTTCAGG CCACCGCCAT CTCGATCATC AATCGCGATG AGATGCTCAT CGACGAGGCA 
CTGGGAGCGC AGGTATTCGT CTTCTTCCAC AGCTTGCTGC TCGAGAAGGA TCTGGTCTAT 
AGCACGCAGT TCTTCTACCG CCGCGTCCAT CTGCTCATCA CGGACTTCAT CGACTTCATG 
AACTCCAAGG TGTCGGAGCT GCGCGGCCGA GCAGACGAGT CGTCACACAC GATCATCAGC 
TTCCTAAACG AGGGTCTGGA ACCGCCGCCC AACTTGGACT CCAACTTTGA GCTGCTCATG 
CTGTGCGTGG CCAAGATGCA CGGCGATCCG CGCGTCACCA TCAGGCTGTG CAACGAGTAC 
TGGGGCCCCG GCGATCCCAA CGGCTCTACG GCATTCAAGA ACACCTCGCG CTCGGTGTCG 
CTGTTCAAGT TCATTAGCTT GGCCAGCGAC CTGCTGCCAC AGACGCTGTT CAAGGCCTAC 
CTCAAGATGA TATCCGGACT TACACGGACC GATTTCTCGG CTCGATGCGC CTTCAACATG 
CTTAGGGTGC CCCAGATGGC CACCGGCGGC AAGTATGCGG TTAGCTGGGA TCACTTCTTC 
ACCACCCTGG GCAACTACTA TACTTCGATG CGCAACGACT TTAACACCAA CATCGGCATG 
AGCGGCGAGA CCATTTACCG CACCCGATCG ACACCAAAGG CCATTACGCA GCGAGAGGCG 
GAGCATCTGG TGGCCGTAAT GGGCATCATA CAGGCGGTGG CGGAACACGA CGAGGTCTCG 
CGCATCATGA TCTGTGACCA TCCCAATTGG CAGACGCCGC AGGTGCTCCT GGGCCTGGTT 
GCGTGCGCCA CGCCGCTTTT TTTGAAGGCG GAGATTCTGC ACACGCTGGC TGCCCTGGCC 
AAATCGAAGG AGACGGCGCG CGTCATCTGG TTCCACCTGG AGGCCTCGCA GATTATACCC 
ACTGTGCCCG TGTCTAGAAG CTATGCCCAG TGCAGTTTGT TGGAGGAGAT GGAACAAATC 
GAGAGCCGTT CGGAGCAGTA TAACCTGACG CGCGGCATCC TCCAATTGCT CTACACACTG 
ATGACCACCA ATCTGCCGAA GAGTTTGGGC GCCGGGCAAC GGACACCGGC CTACGAAGGC 
TATCTAAAGT CCATGATAAA TTCGGTGTTG CTCAAGTTTT ACAATCGCGC GTACAAGGTT 
CCCTCCGAGA AGTGGCAAGT TGGCGCCCAG TGCCTGAAAC TGCTCTACTA CCTACTGGCC 
ACCTATCGGC CGTCCGCAAT GGATTTTCTG GAGACAGTCG ATGAACCTCC CTATCCGGGC 
TTTCACGTTA TGATGCAGCT GCAGCTGAAG TCCGAAATGT TGCAGCTGCT GCTGTGCATT 
GTGGAGGAGG TGCGTGAGCG GCTGGACAAC TACAATCGCT TTCAGGGCAA GAAGCTACTC 
GAGGAGTGCT CATTGTACGC ACTGCTGATC CTGGAAGCCG CATTGGCGAA ACAGAACGCC 
TTCTTTGAGG CCCACTCGGC GGCCAATTGC CCCATTTTGC TGTCGGGCCT GAATCGCATG 
CTCCTGGATC TGAATCCACG CAGTCGCAAG CCCGATCACG TGCTGAATAT CATTAAGTAT 
GTAACCTATA ATAGCTGGCT GCCGCGGCAC GCCCTGGCCG CCATTAAGAT TCTGGCTTCG 
GTCACCCAGC TGCCGAATGT CTCCACCCAG ATTCTCAGCA TGTACGGTCA GGGCAGCAAT 
GAGAAGCTCG AGATTCGTCA GGGTTTCGTC GAGTGCCTGG AAATGGAGGT GTGCGTGGGC 
AAGCACGACG ACGATTTGTT GGACCAACTG GCCCTCAACA ATCACGTTCC GTATCTGGGA 
TTCGGCGATG ATCTGGACAA TGAGCGAGAG ATGAGCGGGG AACGCGAATA TTCAACTATT 
GAATCGCAAC TGGAGCTGCA GGCGGGGGAT GGCGCGCTGG AGAGCAAACC GGCTAGCATT 
GAGCTGCAAC TGAAAGAGGC CATTATCAAG CTGTTCGAGA TGAATCTTAG CCAGCAGTTG 
CCCAACTTTG TGTACTTCCT GCTCGGCGTG GATGTGCTGC GCGAATTTAT GGCCAACGGA 
CGGCAGCACC TGGCCATCGA GATGCAAAGC TCGTGCGTGA ACTCCGTAGT GCTGTTGCTG 
GAAAAGTACA TGGATAAACA GCGTCACAGC GATAAGTACT GCGAGCACAC GGCGAGAATC 
GTGGAGCGCA TTTACCATCT GTTCCATGGA CTCTGCGCCA ATCGCCGCAC CGCGGAGACG 
ATACTGCGCT ACTTCCGGCT AACGTGCAGC GACTTCCTGC TGCGTCACCT ACGCTCGCTG 
CCCTTCCGGC AGCATCGCGA GGATCATGTG CTGCACGCCA TGGGCCACCT GCTCAACTGC 
GTCTCTATCG ATGTCAAACT GGCGGCCAAA CATGGCCAGA TGACGCGCTT CAACCAGATG 
TGCGACATTT TGCTAATTGG AAACGGAATG GAGCGTTCAT CCCATGGCCT GTCGATGGAA 
TTGGGCCACA GCCTGATTTC ACAGCCGTCG TCGTCTTTCC TCGCCATGGA TGTGCTACCG 
GCCGGTGGAT CGATAAGTGC CGTGGCACAT GGGGCTGGTT CGGCGTCTCT CGGAGCTCCA 
AACACTGGGG TCAAATCACT TAAGCCATCG CTGCTGCAGG AGACATCGCA GGGACTGCAT 
GTCACCCGAC TGCTAGATAT TCTCGTCCTG GAGGCGGGCA CGTTGAGCCA GCCGCAGCTG 
GAGTTCTTCG ACGGGCATCT GATCACTCAA CTGCTGCGCG ACTGCGAGGC ATCCGCCGAA 
GCTGGCGCCA ATTCCAGGGC CAACTTGATC AACGTGCGCA AACTGTACTA CATTCTCACC 
GACGAGCTGT ACATGGTGCA GAGCATCATT GCCAGCGGCC AGCGGAAGGC CATTTCCACG 
GAAATCATGC TGCTGCTCAA TCACGCCGTC AACATAAATC GCGTGCGGAC ACAGCGCTGC 
GCCACGCTGG CCTTCATGGC AGCATGGGGT CAACTGGTGC AGGTGCTCTT TAGCAACATG 
CCCGATGCCG TGCTTCCGGC GACGCAGCGT CGCCAGCACA TCATCGATAT CGTCGAGAAG 
GTACTCATCA AGGTGCAGCC CATACAGCCC ATCATCGAGA TATCCGTACA GGTGAGCGAG 
ACGGTGCTGC TGCTGCTTGC CAATTTGCGC TACTGCTGCT ACCAGGCCGA GGACCAGAGC 
CCCGAGGATC TGGCCGCCGA GGATTCGCTC AGCAATGGCA ATGGCAACGC CATCGGCAAC 
GATTCGCAGG TGAACGCATT GTGTCTGGGC CAGAGGGCAA TCGGAACTGG TTCGGATGGC 
GGCAGTGGAG GTGGACGGGA TATCGGTTCC GGTGGCAACA GCAGCAATCT GCGATTCATT 
CTCAAGAACC TCGTCCAGTG GATCATGATC AGTGAGGTGA AGTCGCAGAA GCTGCGCATC 
AATCTGTACA GTTCGCTGCT GAATTGCCTG CGGATCGCGA AGCGACTGCG CACCGACGAG 
CATCTGGAGT ACCAGGAGAC ATTGATTTCG CGCCAGGAGA GTGCACGCAC GTACAACAAG 
GAGCAGCGGC GCGACGATCG CCTGCGGCTG AAGGCCATGG CGGCGGAAGT GATTGGCACG 
TTCGGCGAGA AGCTGATCGA CACCATCTGC CACGATGCGG TGACCGGCCA CGATGTGTGC 
CGCATGCTGG CGCTGGCCTG CCTGGACATG ATCTCCGAGC TGCAGGCGGT GAGCACGCTA 
TGCGACTTTG TGGCATCGCG TGGCTACCTC AAGCACATAC TGGAAAGCCT TGATCAGTCC 
AGTACGGCGC TTTGCGGGAT TCTGCAGCCC GTGCCGGATC ACCTGAGGCC GCTCTACGTC 
TACGAGTCGC GCATGGCCTT CCTCACGCGC ATGGCGAACA GCAATGTGGG AGCCCGGCTC 
CTCCTCACCG AGCAGGCGCT GGGCGTGCTT TCGAATATGA AGGTCTATGA CCAGCAGCCC 
GACCTGAAGT CCAGCGAACT GAATCGTAAC GAGCCGCAGA CCTTCCTCCC GTCCATCGAT 
GACCGCTTCC GGTCGATCCT CCTGCCGGCG CTGAGCCTCT GCGATGCCAT TGTCAACTCG 
CTGGGTCCGC GCAACAACTC CGCCGCCGTG CAGGTGCTTA ACTTTCTGTT CGCCCACATC 
GACATGGTCG AGGCCATGTT GCGCTCGGCC ACGCCGTATA TGGACCTCGG CCACCTGCAG 
CAGGTGGCGG TCATTAGCAA TCTGTTTGCC CGCGCCTCCA CGCACGAGTT GACCGCGCTG 
GAGGATAGCG TGCAGCTGGA TTTGCGAAAT CGCCTGGGCC GCGTGCAGCA GCTGATGATC 
GTGGTGTTCG GACGCTTTTG CGTAAGCGAG CCCACCATTC GTCGCATGCT GCAGCAGGAT 
GAGGAGCAGC AGACCAACCC CACCGATGAC TCGAAGAGGC TACGTGTCAA GTATTTTCTG 
GATATAGCAG CCAACGTGTC GCTCTACTGC CGCAACGTGG TCACCAGCCA TTCGAAGGAC 
AGCATGACCT CCAAATACTT GCTGACCACC GTGATCAATG ATGTAACGCT GCTCACTGGC 
AAAATGTCCA GCAAAAAGCT GACGACCATC ATGCACACCA TCCTCAATCA GCTGAAGGGC 
TCCATCGGCT ACTACCTTTC GCAGAAGTCG ATCGCCGACA ATCTCCTCCA GCAACGCGCC 
TCCCTGCCCA ACATCAGCTT TGGGCCCAAC GGTAAACAAA GCTACATAGA ACTGAGCCAG 
CGATACAACG AGAAGCGCAG CGAACTGAGG CAGGCCGTCT TCATTGCCGA GCAGAATCTG 
TATCTGCTGT GGATCCATCT GGACTTCTAT CTGCGCAACA CCATCGACTA CGCCAACGAA 
AACAGGAACG CCATCAACGA GAGCAACATG GACGACGACA ATGACATGTC CGTGCTGAAC 
GCCTCGCAGG AAGAGATCGT GCAGCTCAAG CAGCTGCTCA TCTCCACATT CAACGAGACA 
TTCTGCACGC AGCTAATCAA CGCCAGCGAG GATTACTCGA TTAAGTGCAA AGGCTTCAAT 
GGCTCGCTGC TGCGACGCAT TAAGGCGCTC GTCCAGTTCG CCCCCATCAC TGCCAACGAT 
GTCAATTCCA GCTTCGATTC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nup205 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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