para cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	para
Entrez Gene ID	32619
Full Name	paralytic
Synonyms	CG9907,DmNa[[V]],DmNa[[v]],DmNa[[v]]1,DmNav1,Dmel\CG9907,Ocd,bas,bss,l(1)14Da,l(1)ESHS48,lincRNA.S9469,olfD,par,sbl,sbl-1
General protein information	Preferred Names paralytic Names CG9907 gene product from transcript CG9907-RBG CG9907-PA CG9907-PAA CG9907-PAB CG9907-PAC CG9907-PAD CG9907-PAE CG9907-PAF CG9907-PAG CG9907-PAH CG9907-PAI CG9907-PAJ CG9907-PAK CG9907-PAL CG9907-PAM CG9907-PAN CG9907-PAO CG9907-PAP CG9907-PAQ CG9907-PAR CG9907-PAS CG9907-PAT CG9907-PAU CG9907-PAV CG9907-PAW CG9907-PAX CG9907-PAY CG9907-PAZ CG9907-PB CG9907-PBA CG9907-PBB CG9907-PBC CG9907-PBD CG9907-PBE CG9907-PBF CG9907-PBG CG9907-PBH CG9907-PC CG9907-PD CG9907-PE CG9907-PF CG9907-PG CG9907-PH CG9907-PI CG9907-PJ CG9907-PK CG9907-PL CG9907-PM CG9907-PN CG9907-PO CG9907-PP CG9907-PQ CG9907-PR CG9907-PS CG9907-PT CG9907-PU CG9907-PV CG9907-PW CG9907-PX CG9907-PY CG9907-PZ Out cold Out-cold bang senseless bang sensitive bang-senseless bangsenseless para-PA para-PAA para-PAB para-PAC para-PAD para-PAE para-PAF para-PAG para-PAH para-PAI para-PAJ para-PAK para-PAL para-PAM para-PAN para-PAO para-PAP para-PAQ para-PAR para-PAS para-PAT para-PAU para-PAV para-PAW para-PAX para-PAY para-PAZ para-PB para-PBA para-PBB para-PBC para-PBD para-PBE para-PBF para-PBG para-PBH para-PC para-PD para-PE para-PF para-PG para-PH para-PI para-PJ para-PK para-PL para-PM para-PN para-PO para-PP para-PQ para-PR para-PS para-PT para-PU para-PV para-PW para-PX para-PY para-PZ paralyzed smell-blind smellblind
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14C8-16A2\|1-55 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201704.1	NP_001188633.1	paralytic, isoform AL
NM_001201683.1	NP_001188612.1	paralytic, isoform Q
NM_001201671.1	NP_001188600.1	paralytic, isoform E
NM_001201699.1	NP_001188628.1	paralytic, isoform AG
NM_001201703.3	NP_001188632.1	paralytic, isoform AK
NM_001298403.1	NP_001285332.1	paralytic, isoform BG
NM_001298404.1	NP_001285333.1	paralytic, isoform BH
NM_001201720.1	NP_001188649.1	paralytic, isoform BB
NM_001201715.1	NP_001188644.1	paralytic, isoform AW
NM_001201674.1	NP_001188603.1	paralytic, isoform H
NM_001201684.1	NP_001188613.1	paralytic, isoform R
NM_001201701.1	NP_001188630.1	paralytic, isoform AI
NM_001201712.1	NP_001188641.1	paralytic, isoform AT
NM_001201672.1	NP_001188601.1	paralytic, isoform F
NM_001201721.1	NP_001188650.1	paralytic, isoform BC
NM_001201691.1	NP_001188620.1	paralytic, isoform Y
NM_001201688.1	NP_001188617.1	paralytic, isoform V
NM_001201719.1	NP_001188648.1	paralytic, isoform BA
NM_001201678.1	NP_001188607.1	paralytic, isoform L
NM_078647.4	NP_523371.2	paralytic, isoform A
NM_001201705.1	NP_001188634.1	paralytic, isoform AM
NM_001201687.1	NP_001188616.1	paralytic, isoform U
NM_001201689.1	NP_001188618.1	paralytic, isoform W
NM_001201676.1	NP_001188605.1	paralytic, isoform J
NM_001042816.2	NP_001036281.1	paralytic, isoform C
NM_001201713.1	NP_001188642.1	paralytic, isoform AU
NM_001201673.1	NP_001188602.1	paralytic, isoform G
NM_001144733.2	NP_001138205.1	paralytic, isoform D
NM_001201716.1	NP_001188645.1	paralytic, isoform AX
NM_001201711.1	NP_001188640.1	paralytic, isoform AS
NM_001201686.1	NP_001188615.1	paralytic, isoform T
NM_001201718.1	NP_001188647.1	paralytic, isoform AZ
NM_001201693.1	NP_001188622.1	paralytic, isoform AA
NM_001201679.1	NP_001188608.1	paralytic, isoform M
NM_001201706.1	NP_001188635.1	paralytic, isoform AN
NM_001201694.1	NP_001188623.1	paralytic, isoform AB
NM_001201692.1	NP_001188621.1	paralytic, isoform Z
NM_001201690.1	NP_001188619.1	paralytic, isoform X
NM_001201708.1	NP_001188637.1	paralytic, isoform AP
NM_001201681.1	NP_001188610.1	paralytic, isoform O
NM_001201723.1	NP_001188652.1	paralytic, isoform BE
NM_001201680.1	NP_001188609.1	paralytic, isoform N
NM_001201695.2	NP_001188624.1	paralytic, isoform AC
NM_001201710.1	NP_001188639.1	paralytic, isoform AR
NM_001201714.1	NP_001188643.1	paralytic, isoform AV
NM_001201717.1	NP_001188646.1	paralytic, isoform AY
NM_001201707.1	NP_001188636.1	paralytic, isoform AO
NM_001042815.2	NP_001036280.1	paralytic, isoform B
NM_001201677.1	NP_001188606.1	paralytic, isoform K
NM_001201722.1	NP_001188651.1	paralytic, isoform BD
NM_001201682.1	NP_001188611.1	paralytic, isoform P
NM_001201675.1	NP_001188604.1	paralytic, isoform I
NM_001201700.1	NP_001188629.1	paralytic, isoform AH
NM_001201697.1	NP_001188626.1	paralytic, isoform AE
NM_001272690.1	NP_001259619.1	paralytic, isoform BF
NM_001201696.1	NP_001188625.1	paralytic, isoform AD
NM_001201709.1	NP_001188638.1	paralytic, isoform AQ
NM_001201685.1	NP_001188614.1	paralytic, isoform S
NM_001201702.1	NP_001188631.1	paralytic, isoform AJ
NM_001201698.1	NP_001188627.1	paralytic, isoform AF

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCN2A	NP_001035233.1
Gallus gallus (chicken)	SCN2A	XP_004942844.1
	SCN2A	XP_001099916.1
Canis lupus familiaris (dog)	SCN2A	XP_535939.2
Rattus norvegicus (Norway rat)	Scn2a1	NP_036779.1
Drosophila melanogaster (fruit fly)	para	NP_001188624.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004707	XP_554647.3
Xenopus tropicalis (tropical clawed frog)	scn3a	XP_002939317.2
Mus musculus (house mouse)	Scn2a1	NP_001092768.1
Bos taurus (cattle)	SCN2A	NP_001137581.1
Pan troglodytes (chimpanzee)	SCN2A	XP_001153227.1
Danio rerio (zebrafish)	scn1lab	NP_001038360.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identified Shu, a Drosophila mutation causing severe behavioral defects, as a novel hypermorphic allele of the Nav channel gene para. Results suggest that the Shu Nav channels cause an increase in sodium currents, and that this defect is compensated by reduced para function, leading to a more balanced neuronal output.
Title: Lithium-Responsive Seizure-Like Hyperexcitability Is Caused by a Mutation in the

Trp404 and His405 as a putative point of Av3 interaction with DmNav1.
Title: The specificity of Av3 sea anemone toxin for arthropods is determined at linker DI/SS2-S6?in the pore module of target sodium channels.

analysis revealed that 2 variant-specific amino acid changes, I260T in the S4-S5 linker of domain I (ILS4-S5) and A1731V in the voltage sensor S4 of domain IV (IVS4), contribute to the persistent sodium current (INaP)
Title: Sequence variations at I260 and A1731 contribute to persistent currents in Drosophila sodium channels.

The para(bss1) has not responded to seizure suppressor mutations identified in screens based on the Drosophila mutants eas protein and sda protein.
Title: Drosophila as a model for intractable epilepsy: gilgamesh suppresses seizures in para(bss1) heterozygote flies.

results indicate that increased synaptic activity influences the decision to splice in exon L, which, in turn, may promote seizure.
Title: Activity-dependent alternative splicing increases persistent sodium current and promotes seizure.

The following para gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the para cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03644	NM_001201710.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AR, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa03649	NM_001201721.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant BC, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03657	NM_001201723.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant BE, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03645	NM_001201714.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AV, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa03646	NM_001201713.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AU, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa01805	NM_001144733.2 Latest version!	Drosophila melanogaster paralytic (para), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01806	NM_001201689.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant W, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01807	NM_001201684.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant R, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01808	NM_001201673.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01809	NM_001201688.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant V, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01810	NM_001201708.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AP, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01811	NM_001201680.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant N, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01812	NM_001201678.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant L, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01813	NM_001201685.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant S, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01814	NM_001201679.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant M, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01815	NM_001201686.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant T, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01816	NM_001201707.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AO, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01817	NM_001201676.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01818	NM_001201690.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant X, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01819	NM_001201691.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant Y, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01820	NM_001201671.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01821	NM_001201697.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AE, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01822	NM_001201687.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant U, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01823	NM_001201706.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AN, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01824	NM_001201682.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant P, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01825	NM_001272690.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant BF, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa01826	NM_001201672.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03028	NM_078647.4 Latest version!	Drosophila melanogaster paralytic (para), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03028	NM_001298403.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant BG, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03029	NM_001042815.2 Latest version!	Drosophila melanogaster paralytic (para), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03030	NM_001042816.2 Latest version!	Drosophila melanogaster paralytic (para), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03634	NM_001201681.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant O, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03642	NM_001201703.3 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AK, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03642	NM_001201674.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03643	NM_001201693.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AA, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03643	NM_001201695.2 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AC, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03647	NM_001201712.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AT, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03648	NM_001201711.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AS, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03650	NM_001201715.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AW, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03651	NM_001201705.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AM, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03652	NM_001201701.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AI, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03653	NM_001201696.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AD, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03654	NM_001201704.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AL, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03655	NM_001201718.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AZ, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03656	NM_001201699.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AG, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03658	NM_001201716.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AX, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03659	NM_001201722.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant BD, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03660	NM_001201720.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant BB, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03661	NM_001201700.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AH, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03662	NM_001201702.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AJ, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03663	NM_001201717.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AY, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03664	NM_001201698.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AF, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03665	NM_001201719.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant BA, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03666	NM_001201683.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant Q, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03667	NM_001201677.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant K, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03668	NM_001201694.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AB, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03669	NM_001201692.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant Z, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03670	NM_001201675.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03671	NM_001201709.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant AQ, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa21502	NM_001298404.1 Latest version!	Drosophila melanogaster paralytic (para), transcript variant BH, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

para ( NM_001201710.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188639.1 Drosophila melanogaster paralytic (para), transcript variant AR, mRNA.

Price & Availability↑

CloneID	OFa03644
Clone ID Related Accession (Same CDS sequence)	NM_001201710.1
Accession Version	NM_001201710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AR
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGCTTGAAG ACCATCGTCG GCGCCGTCAT 
CGAATCGGTG AAGAATCTGC GCGATGTGAT TATCCTGACC ATGTTCTCCC TGTCGGTGTT 
CGCGTTGATG GGCCTACAGA TCTATATGGG CGTGCTCACG CAGAAGTGCA TCAAGAAGTT 
CCCGCTGGAC GGTTCCTGGG GCAATCTGAC CGACGAGAAC TGGGACTATC ACAATCGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188639.1
CDS	269..991
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTELEDHRRRRHRIGEESARCDYPDHVLPVGVRVDGPTDLYGRAHAEVHQEVPAGRFLGQSDRRELGLSQSQ

Target ORF information:

RefSeq Version	NM_001201710.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AR, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201710.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGCTTGAAG ACCATCGTCG GCGCCGTCAT 
CGAATCGGTG AAGAATCTGC GCGATGTGAT TATCCTGACC ATGTTCTCCC TGTCGGTGTT 
CGCGTTGATG GGCCTACAGA TCTATATGGG CGTGCTCACG CAGAAGTGCA TCAAGAAGTT 
CCCGCTGGAC GGTTCCTGGG GCAATCTGAC CGACGAGAAC TGGGACTATC ACAATCGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201721.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188650.1 Drosophila melanogaster paralytic (para), transcript variant BC, mRNA.

Price & Availability↑

CloneID	OFa03649
Clone ID Related Accession (Same CDS sequence)	NM_001201721.1
Accession Version	NM_001201721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform BC
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGTAC 
ACGATACGGA ACGGACGTGG CCGCTTTGGT ATACCCGGTA GCGATCGTAA GCCATTGGTA 
TTGTCAACAT ATCAGGATGC CCAGCAGCAC TTGCCCTATG CCGACGACTC GAATGCCGTC 
ACCCCGATGT CCGAAGAGAA TGGGGCCATC ATAGTGCCCG TGTACTATGG CAATCTAGGC 
TCCCGACACT CATCGTATAC CTCGCATCAG TCCCGAATAT CGTATACCTC ACATGGCGAT 
CTACTCGGCG GCATGGCCGT CATGGGCGTC AGCACAATGA CCAAGGAGAG CAAATTGCGC 
AACCGGAACA CACGCAATCA ATCAGTGGGC GCCACCAATG GCGGCACCAC CTGTCTGGAC 
ACCAATCACA AGCTCGATCA TCGCGACTAC GAAATTGGCC TGGAGTGCAC GGACGAAGCT 
GGCAAGATTA AACATCATGA CAATCCTTTT ATCGAGCCCG TCCAGACACA AACGGTGGTT 
GATATGAAAG ATGTGATGGT CCTGAATGAC ATCATCGAAC AGGCCGCTGG TCGGCACAGT 
CGGGCAAGCG ATCGCGGTGA GGACGATGAC GAGGATGGGC CGACGTTCAA AGACAAGGCA 
CTCGAAGTGA TCCTCAAAGG CATCGATGTG TTTTGTGTGT GGGACTGTTG CTGGGTTTGG 
TTGAAATTTC AGGAGTGGGT ATCGCTCATC GTCTTCGATC CCTTCGTCGA GCTCTTCATC 
ACGCTGTGCA TTGTGGTCAA CACGATGTTC ATGGCAATGG ATCACCACGA TATGAACAAG 
GAGATGGAAC GCGTGCTCAA GAGTGGCAAC TATTTCTTCA CCGCCACCTT TGCCATCGAG 
GCCACCATGA AGCTAATGGC CATGAGCCCC AAGTACTATT TCCAGGAGGG CTGGAACATC 
TTCGACTTCA TTATCGTGGC CCTATCGCTA TTGGAACTGG GACTCGAGGG TGTCCAGGGT 
CTGTCCGTAT TGCGTTCCTT TCGATTGATC ACAAGGACCG CTTTCCGGAT GGCGACCTGC 
CGCGCTGGAA CTTCACCGAC TTTATGCACA GCTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188650.1
CDS	269..2887
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLITRTAFRMATCRAGTSPTLCTAS

Target ORF information:

RefSeq Version	NM_001201721.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant BC, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201721.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGTAC 
ACGATACGGA ACGGACGTGG CCGCTTTGGT ATACCCGGTA GCGATCGTAA GCCATTGGTA 
TTGTCAACAT ATCAGGATGC CCAGCAGCAC TTGCCCTATG CCGACGACTC GAATGCCGTC 
ACCCCGATGT CCGAAGAGAA TGGGGCCATC ATAGTGCCCG TGTACTATGG CAATCTAGGC 
TCCCGACACT CATCGTATAC CTCGCATCAG TCCCGAATAT CGTATACCTC ACATGGCGAT 
CTACTCGGCG GCATGGCCGT CATGGGCGTC AGCACAATGA CCAAGGAGAG CAAATTGCGC 
AACCGGAACA CACGCAATCA ATCAGTGGGC GCCACCAATG GCGGCACCAC CTGTCTGGAC 
ACCAATCACA AGCTCGATCA TCGCGACTAC GAAATTGGCC TGGAGTGCAC GGACGAAGCT 
GGCAAGATTA AACATCATGA CAATCCTTTT ATCGAGCCCG TCCAGACACA AACGGTGGTT 
GATATGAAAG ATGTGATGGT CCTGAATGAC ATCATCGAAC AGGCCGCTGG TCGGCACAGT 
CGGGCAAGCG ATCGCGGTGA GGACGATGAC GAGGATGGGC CGACGTTCAA AGACAAGGCA 
CTCGAAGTGA TCCTCAAAGG CATCGATGTG TTTTGTGTGT GGGACTGTTG CTGGGTTTGG 
TTGAAATTTC AGGAGTGGGT ATCGCTCATC GTCTTCGATC CCTTCGTCGA GCTCTTCATC 
ACGCTGTGCA TTGTGGTCAA CACGATGTTC ATGGCAATGG ATCACCACGA TATGAACAAG 
GAGATGGAAC GCGTGCTCAA GAGTGGCAAC TATTTCTTCA CCGCCACCTT TGCCATCGAG 
GCCACCATGA AGCTAATGGC CATGAGCCCC AAGTACTATT TCCAGGAGGG CTGGAACATC 
TTCGACTTCA TTATCGTGGC CCTATCGCTA TTGGAACTGG GACTCGAGGG TGTCCAGGGT 
CTGTCCGTAT TGCGTTCCTT TCGATTGATC ACAAGGACCG CTTTCCGGAT GGCGACCTGC 
CGCGCTGGAA CTTCACCGAC TTTATGCACA GCTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201723.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188652.1 Drosophila melanogaster paralytic (para), transcript variant BE, mRNA.

Price & Availability↑

CloneID	OFa03657
Clone ID Related Accession (Same CDS sequence)	NM_001201723.1
Accession Version	NM_001201723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform BE
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGAGGACG ATGACGAGGA TGGGCCGACG 
TTCAAAGACA AGGCACTCGA AGTGATCCTC AAAGGCATCG ATGTGTTTTG TGTGTGGGAC 
TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG TGGGTATCGC TCATCGTCTT CGATCCCTTC 
GTCGAGCTCT TCATCACGCT GTGCATTGTG GTCAACACGA TGTTCATGGC AATGGATCAC 
CACGATATGA ACAAGGAGAT GGAACGCGTG CTCAAGAGTG GCAACTATTT CTTCACCGCC 
ACCTTTGCCA TCGAGGCCAC CATGAAGCTA ATGGCCATGA GCCCCAAGTA CTATTTCCAG 
GAGGGCTGGA ACATCTTCGA CTTCATTATC GTGGCCCTAT CGCTATTGGA ACTGGGACTC 
GAGGGTGTCC AGGGTCTGTC CGTATTGCGT TCCTTTCGAT TGATCACAAG GACCGCTTTC 
CGGATGGCGA CCTGCCGCGC TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188652.1
CDS	269..3022
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLITRTAFRMATCRAGTSPTLCTAS

Target ORF information:

RefSeq Version	NM_001201723.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant BE, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201723.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGAGGACG ATGACGAGGA TGGGCCGACG 
TTCAAAGACA AGGCACTCGA AGTGATCCTC AAAGGCATCG ATGTGTTTTG TGTGTGGGAC 
TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG TGGGTATCGC TCATCGTCTT CGATCCCTTC 
GTCGAGCTCT TCATCACGCT GTGCATTGTG GTCAACACGA TGTTCATGGC AATGGATCAC 
CACGATATGA ACAAGGAGAT GGAACGCGTG CTCAAGAGTG GCAACTATTT CTTCACCGCC 
ACCTTTGCCA TCGAGGCCAC CATGAAGCTA ATGGCCATGA GCCCCAAGTA CTATTTCCAG 
GAGGGCTGGA ACATCTTCGA CTTCATTATC GTGGCCCTAT CGCTATTGGA ACTGGGACTC 
GAGGGTGTCC AGGGTCTGTC CGTATTGCGT TCCTTTCGAT TGATCACAAG GACCGCTTTC 
CGGATGGCGA CCTGCCGCGC TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201714.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188643.1 Drosophila melanogaster paralytic (para), transcript variant AV, mRNA.

Price & Availability↑

CloneID	OFa03645
Clone ID Related Accession (Same CDS sequence)	NM_001201714.1
Accession Version	NM_001201714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AV
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GATTATGTGA CCATGGGTAT AGATTTAGGT 
AATCTAGCAG CCCTGCGAAC GTTTAGGGTG CTGCGAGCGC TTAAAACCGT AGCCATTGTG 
CCAGGCTTGA AGACCATCGT CGGCGCCGTC ATCGAATCGG TGAAGAATCT GCGCGATGTG 
ATTATCCTGA CCATGTTCTC CCTGTCGGTG TTCGCGTTGA TGGGCCTACA GATCTATATG 
GGCGTGCTCA CGCAGAAGTG CATCAAGAAG TTCCCGCTGG ACGGTTCCTG GGGCAATCTG 
ACCGACGAGA ACTGGGACTA TCACAATCGC AATAGCTCCA ATTGGTATTC CGAGGACGAG 
GGCATCTCAT TTCCGTTATG CGGCAATATA TCCGGTGCGG GGCAATGCGA CGACGATTAC 
GTGTGCCTGC AGGGGTTTGG TCCGAATCCG AATTATGGCT ACACCAGCTT CGATTCGTTC 
GGATGGGCTT TCCTGTCCGC CTTCCGGCTG ATGACACAGG ACTTCTGGGA GGATCTGTAC 
CAGCTGGTGT TGCGCGCCGC CGGACCATGG CACATGCTGT TCTTTATAGT CATCATCTTC 
CTAGGTTCAT TCTATCTTGT GAATTTGATT TTGGCCATTG TTGCCATGTC GTATGACGAA 
TTGCAAAAGA AGGCCGAAGA AGAAGAGGCT GCCGAAGAGG AGGCGATACG TGAAGCGGAA 
GAAGCTGCCG CCGCCAAAGC GGCCAAGCTG GAGGAGCGGG CCAATGCGCA GGCTCAGGCA 
GCAGCGGATG CGGCTGCCGC CGAAGAGGCT GCACTGCATC CGGAAATGGC CAAGAGTCCG 
ACGTATTCTT GCATCAGCTA TGAGCTATTT GTTGGCGGCG AGAAGGGCAA CGATGACAAC 
AACAAAGAGA AGATGTCCAT TCGGAGCGTC GAGGTGGAGT CGGAGTCGGT GAGCGTTATA 
CAAAGACAAC CAGCACCTAC CACAGCACAC CAAGCTACCA AAGTTCGTAA AGTGAGCACG 
TACACGATAC GGAACGGACG TGGCCGCTTT GGTATACCCG GTAGCGATCG TAAGCCATTG 
GTATTGTCAA CATATCAGGA TGCCCAGCAG CACTTGCCCT ATGCCGACGA CTCGAATGCC 
GTCACCCCGA TGTCCGAAGA GAATGGGGCC ATCATAGTGC CCGTGTACTA TGGCAATCTA 
GGCTCCCGAC ACTCATCGTA TACCTCGCAT CAGTCCCGAA TATCGTATAC CTCACATGGC 
GATCTACTCG GCGGCATGGC CGTCATGGGC GTCAGCACAA TGACCAAGGA GAGCAAATTG 
CGCAACCGGA ACACACGCAA TCAATCAGTG GGCGCCACCA ATGGCGGCAC CACCTGTCTG 
GACACCAATC ACAAGCTCGA TCATCGCGAC TACGAAATTG GCCTGGAGTG CACGGACGAA 
GCTGGCAAGA TTAAACATCA TGACAATCCT TTTATCGAGC CCGTCCAGAC ACAAACGGTG 
GTTGATATGA AAGATGTGAT GGTCCTGAAT GACATCATCG AACAGGCCGC TGGTCGGCAC 
AGTCGGGCAA GCGATCGCGG TGAGGACGAT GACGAGGATG GGCCGACGTT CAAAGACAAG 
GCACTCGAAG TGATCCTCAA AGGCATCGAT GTGTTTTGTG TGTGGGACTG TTGCTGGGTT 
TGGTTGAAAT TTCAGGAGTG GGTATCGCTC ATCGTCTTCG ATCCCTTCGT CGAGCTCTTC 
ATCACGCTGT GCATTGTGGT CAACACGATG TTCATGGCAA TGGATCACCA CGATATGAAC 
AAGGAGATGG AACGCGTGCT CAAGAGTGGC AACTATTTCT TCACCGCCAC CTTTGCCATC 
GAGGCCACCA TGAAGCTAAT GGCCATGAGC CCCAAGTACT ATTTCCAGGA GGGCTGGAAC 
ATCTTCGACT TCATTATCGT GGCCCTATCG CTATTGGAAC TGGGACTCGA GGGTGTCCAG 
GGTCTGTCCG TATTGCGTTC CTTTCGATTG CTGCGTGTAT TCAAACTGGC CAAGTCTTGG 
CCCACACTTA ATTTACTCAT TTCGATTATG GGACGCACCA TGGGCGCTTT GGGTAATCTG 
ACATTTGTAC TTTGCATTAT CATCTTCATC TTTGCGGTGA TGGGAATGCA ACTGTTCGGA 
AAGAATTATC ATGATCACAA GGACCGCTTT CCGGATGGCG ACCTGCCGCG CTGGAACTTC 
ACCGACTTTA TGCACAGCTT CATGATCGTG TTCCGGGTGC TCTGCGGAGA ATGGATCGAG 
TCCATGTGGG ACTGCATGTA CGTGGGCGAT GTCTCGTGCA TTCCCTTCTT CTTGGCCACC 
GTTGTCATCG GCAATCTTGT GGTACTTAAC CTTTTCTTAG CCTTGCTTTT GTCCAATTTT 
GGCTCATCTA GCTTATCAGC GCCGACTGCC GATAACGATA CGAATAAAAT AGCCGAGGCC 
TTCAATCGAA TTGGCCGATT TAAAAGTTGG GTTAAGCGTA ATATTGCTGA TTGTTTCAAG 
TTAATACGTA ACAAATTGAC AAATCAAATA AGTGATCAAC CATCAGGCAT TAGAAGATGT 
ACATCTGCCA CAAAGACCCA TACTGCAGGA TATTTTATAC TATATGGACA GAATATTTAC 
GGTTATATTC TTCTTGGAAA TGTTAATCAA GTGGTTGGCG CTCGGCTTCA AAGTGTACTT 
CACCAACGCG TGGTGTTGGC TCGATTTCGT GATTGTCATG GTATCGCTTA TCAACTTCGT 
TGCTTCACTT GTTGGAGCTG GTGGTATTCA AGCCTTCAAG ACTATGCGAA CGTTAAGAGC 
ACTGAGACCA CTACGTGCCA TGTCCCGTAT GCAGGGCATG AGGGTCGTCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188643.1
CDS	269..3622
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTDYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGIRRCTSATKTHTAGYFILYGQNIYGYILLGNVNQVVGARLQSVLHQRVVLARFRDCHGIAYQLRCFTCWSWWYSSLQDYANVKSTETTTCHVPYAGHEGRR

Target ORF information:

RefSeq Version	NM_001201714.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AV, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201714.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GATTATGTGA CCATGGGTAT AGATTTAGGT 
AATCTAGCAG CCCTGCGAAC GTTTAGGGTG CTGCGAGCGC TTAAAACCGT AGCCATTGTG 
CCAGGCTTGA AGACCATCGT CGGCGCCGTC ATCGAATCGG TGAAGAATCT GCGCGATGTG 
ATTATCCTGA CCATGTTCTC CCTGTCGGTG TTCGCGTTGA TGGGCCTACA GATCTATATG 
GGCGTGCTCA CGCAGAAGTG CATCAAGAAG TTCCCGCTGG ACGGTTCCTG GGGCAATCTG 
ACCGACGAGA ACTGGGACTA TCACAATCGC AATAGCTCCA ATTGGTATTC CGAGGACGAG 
GGCATCTCAT TTCCGTTATG CGGCAATATA TCCGGTGCGG GGCAATGCGA CGACGATTAC 
GTGTGCCTGC AGGGGTTTGG TCCGAATCCG AATTATGGCT ACACCAGCTT CGATTCGTTC 
GGATGGGCTT TCCTGTCCGC CTTCCGGCTG ATGACACAGG ACTTCTGGGA GGATCTGTAC 
CAGCTGGTGT TGCGCGCCGC CGGACCATGG CACATGCTGT TCTTTATAGT CATCATCTTC 
CTAGGTTCAT TCTATCTTGT GAATTTGATT TTGGCCATTG TTGCCATGTC GTATGACGAA 
TTGCAAAAGA AGGCCGAAGA AGAAGAGGCT GCCGAAGAGG AGGCGATACG TGAAGCGGAA 
GAAGCTGCCG CCGCCAAAGC GGCCAAGCTG GAGGAGCGGG CCAATGCGCA GGCTCAGGCA 
GCAGCGGATG CGGCTGCCGC CGAAGAGGCT GCACTGCATC CGGAAATGGC CAAGAGTCCG 
ACGTATTCTT GCATCAGCTA TGAGCTATTT GTTGGCGGCG AGAAGGGCAA CGATGACAAC 
AACAAAGAGA AGATGTCCAT TCGGAGCGTC GAGGTGGAGT CGGAGTCGGT GAGCGTTATA 
CAAAGACAAC CAGCACCTAC CACAGCACAC CAAGCTACCA AAGTTCGTAA AGTGAGCACG 
TACACGATAC GGAACGGACG TGGCCGCTTT GGTATACCCG GTAGCGATCG TAAGCCATTG 
GTATTGTCAA CATATCAGGA TGCCCAGCAG CACTTGCCCT ATGCCGACGA CTCGAATGCC 
GTCACCCCGA TGTCCGAAGA GAATGGGGCC ATCATAGTGC CCGTGTACTA TGGCAATCTA 
GGCTCCCGAC ACTCATCGTA TACCTCGCAT CAGTCCCGAA TATCGTATAC CTCACATGGC 
GATCTACTCG GCGGCATGGC CGTCATGGGC GTCAGCACAA TGACCAAGGA GAGCAAATTG 
CGCAACCGGA ACACACGCAA TCAATCAGTG GGCGCCACCA ATGGCGGCAC CACCTGTCTG 
GACACCAATC ACAAGCTCGA TCATCGCGAC TACGAAATTG GCCTGGAGTG CACGGACGAA 
GCTGGCAAGA TTAAACATCA TGACAATCCT TTTATCGAGC CCGTCCAGAC ACAAACGGTG 
GTTGATATGA AAGATGTGAT GGTCCTGAAT GACATCATCG AACAGGCCGC TGGTCGGCAC 
AGTCGGGCAA GCGATCGCGG TGAGGACGAT GACGAGGATG GGCCGACGTT CAAAGACAAG 
GCACTCGAAG TGATCCTCAA AGGCATCGAT GTGTTTTGTG TGTGGGACTG TTGCTGGGTT 
TGGTTGAAAT TTCAGGAGTG GGTATCGCTC ATCGTCTTCG ATCCCTTCGT CGAGCTCTTC 
ATCACGCTGT GCATTGTGGT CAACACGATG TTCATGGCAA TGGATCACCA CGATATGAAC 
AAGGAGATGG AACGCGTGCT CAAGAGTGGC AACTATTTCT TCACCGCCAC CTTTGCCATC 
GAGGCCACCA TGAAGCTAAT GGCCATGAGC CCCAAGTACT ATTTCCAGGA GGGCTGGAAC 
ATCTTCGACT TCATTATCGT GGCCCTATCG CTATTGGAAC TGGGACTCGA GGGTGTCCAG 
GGTCTGTCCG TATTGCGTTC CTTTCGATTG CTGCGTGTAT TCAAACTGGC CAAGTCTTGG 
CCCACACTTA ATTTACTCAT TTCGATTATG GGACGCACCA TGGGCGCTTT GGGTAATCTG 
ACATTTGTAC TTTGCATTAT CATCTTCATC TTTGCGGTGA TGGGAATGCA ACTGTTCGGA 
AAGAATTATC ATGATCACAA GGACCGCTTT CCGGATGGCG ACCTGCCGCG CTGGAACTTC 
ACCGACTTTA TGCACAGCTT CATGATCGTG TTCCGGGTGC TCTGCGGAGA ATGGATCGAG 
TCCATGTGGG ACTGCATGTA CGTGGGCGAT GTCTCGTGCA TTCCCTTCTT CTTGGCCACC 
GTTGTCATCG GCAATCTTGT GGTACTTAAC CTTTTCTTAG CCTTGCTTTT GTCCAATTTT 
GGCTCATCTA GCTTATCAGC GCCGACTGCC GATAACGATA CGAATAAAAT AGCCGAGGCC 
TTCAATCGAA TTGGCCGATT TAAAAGTTGG GTTAAGCGTA ATATTGCTGA TTGTTTCAAG 
TTAATACGTA ACAAATTGAC AAATCAAATA AGTGATCAAC CATCAGGCAT TAGAAGATGT 
ACATCTGCCA CAAAGACCCA TACTGCAGGA TATTTTATAC TATATGGACA GAATATTTAC 
GGTTATATTC TTCTTGGAAA TGTTAATCAA GTGGTTGGCG CTCGGCTTCA AAGTGTACTT 
CACCAACGCG TGGTGTTGGC TCGATTTCGT GATTGTCATG GTATCGCTTA TCAACTTCGT 
TGCTTCACTT GTTGGAGCTG GTGGTATTCA AGCCTTCAAG ACTATGCGAA CGTTAAGAGC 
ACTGAGACCA CTACGTGCCA TGTCCCGTAT GCAGGGCATG AGGGTCGTCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201713.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188642.1 Drosophila melanogaster paralytic (para), transcript variant AU, mRNA.

Price & Availability↑

CloneID	OFa03646
Clone ID Related Accession (Same CDS sequence)	NM_001201713.1
Accession Version	NM_001201713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AU
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GATTATGTGA CCATGGGTAT AGATTTAGGT 
AATCTAGCAG CCCTGCGAAC GTTTAGGGTG CTGCGAGCGC TTAAAACCGT AGCCATTGTG 
CCAGGCTTGA AGACCATCGT CGGCGCCGTC ATCGAATCGG TGAAGAATCT GCGCGATGTG 
ATTATCCTGA CCATGTTCTC CCTGTCGGTG TTCGCGTTGA TGGGCCTACA GATCTATATG 
GGCGTGCTCA CGCAGAAGTG CATCAAGAAG TTCCCGCTGG ACGGTTCCTG GGGCAATCTG 
ACCGACGAGA ACTGGGACTA TCACAATCGC AATAGCTCCA ATTGGTATTC CGAGGACGAG 
GGCATCTCAT TTCCGTTATG CGGCAATATA TCCGGTGCGG GGCAATGCGA CGACGATTAC 
GTGTGCCTGC AGGGGTTTGG TCCGAATCCG AATTATGGCT ACACCAGCTT CGATTCGTTC 
GGATGGGCTT TCCTGTCCGC CTTCCGGCTG ATGACACAGG ACTTCTGGGA GGATCTGTAC 
CAGCTGGTGT TGCGCGCCGC CGGACCATGG CACATGCTGT TCTTTATAGT CATCATCTTC 
CTAGGTTCAT TCTATCTTGT GAATTTGATT TTGGCCATTG TTGCCATGTC GTATGACGAA 
TTGCAAAAGA AGGCCGAAGA AGAAGAGGCT GCCGAAGAGG AGGCGATACG TGAAGCGGAA 
GAAGCTGCCG CCGCCAAAGC GGCCAAGCTG GAGGAGCGGG CCAATGCGCA GGCTCAGGCA 
GCAGCGGATG CGGCTGCCGC CGAAGAGGCT GCACTGCATC CGGAAATGGC CAAGAGTCCG 
ACGTATTCTT GCATCAGCTA TGAGCTATTT GTTGGCGGCG AGAAGGGCAA CGATGACAAC 
AACAAAGAGA AGATGTCCAT TCGGAGCGTC GAGGTGGAGT CGGAGTCGGT GAGCGTTATA 
CAAAGACAAC CAGCACCTAC CACAGCACAC CAAGCTACCA AAGTTCGTAA AGTGAGCACG 
TACACGATAC GGAACGGACG TGGCCGCTTT GGTATACCCG GTAGCGATCG TAAGCCATTG 
GTATTGTCAA CATATCAGGA TGCCCAGCAG CACTTGCCCT ATGCCGACGA CTCGAATGCC 
GTCACCCCGA TGTCCGAAGA GAATGGGGCC ATCATAGTGC CCGTGTACTA TGGCAATCTA 
GGCTCCCGAC ACTCATCGTA TACCTCGCAT CAGTCCCGAA TATCGTATAC CTCACATGGC 
GATCTACTCG GCGGCATGGC CGTCATGGGC GTCAGCACAA TGACCAAGGA GAGCAAATTG 
CGCAACCGGA ACACACGCAA TCAATCAGTG GGCGCCACCA ATGGCGGCAC CACCTGTCTG 
GACACCAATC ACAAGCTCGA TCATCGCGAC TACGAAATTG GCCTGGAGTG CACGGACGAA 
GCTGGCAAGA TTAAACATCA TGACAATCCT TTTATCGAGC CCGTCCAGAC ACAAACGGTG 
GTTGATATGA AAGATGTGAT GGTCCTGAAT GACATCATCG AACAGGCCGC TGGTCGGCAC 
AGTCGGGCAA GCGATCGCGG TGTCTCCGTT TACTATTTCC CAACAGAGGA CGATGACGAG 
GATGGGCCGA CGTTCAAAGA CAAGGCACTC GAAGTGATCC TCAAAGGCAT CGATGTGTTT 
TGTGTGTGGG ACTGTTGCTG GGTTTGGTTG AAATTTCAGG AGTGGGTATC GCTCATCGTC 
TTCGATCCCT TCGTCGAGCT CTTCATCACG CTGTGCATTG TGGTCAACAC GATGTTCATG 
GCAATGGATC ACCACGATAT GAACAAGGAG ATGGAACGCG TGCTCAAGAG TGGCAACTAT 
TTCTTCACCG CCACCTTTGC CATCGAGGCC ACCATGAAGC TAATGGCCAT GAGCCCCAAG 
TACTATTTCC AGGAGGGCTG GAACATCTTC GACTTCATTA TCGTGGCCCT ATCGCTATTG 
GAACTGGGAC TCGAGGGTGT CCAGGGTCTG TCCGTATTGC GTTCCTTTCG ATTGCTGCGT 
GTATTCAAAC TGGCCAAGTC TTGGCCCACA CTTAATTTAC TCATTTCGAT TATGGGACGC 
ACCATGGGCG CTTTGGGTAA TCTGACATTT GTACTTTGCA TTATCATCTT CATCTTTGCG 
GTGATGGGAA TGCAACTGTT CGGAAAGAAT TATCATGATC ACAAGGACCG CTTTCCGGAT 
GGCGACCTGC CGCGCTGGAA CTTCACCGAC TTTATGCACA GCTTCATGAT CGTGTTCCGG 
GTGCTCTGCG GAGAATGGAT CGAGTCCATG TGGGACTGCA TGTACGTGGG CGATGTCTCG 
TGCATTCCCT TCTTCTTGGC CACCGTTGTC ATCGGCAATC TTGTGGTACT TAACCTTTTC 
TTAGCCTTGC TTTTGTCCAA TTTTGGCTCA TCTAGCTTAT CAGCGCCGAC TGCCGATAAC 
GATACGAATA AAATAGCCGA GGCCTTCAAT CGAATTGGCC GATTTAAAAG TTGGGTTAAG 
CGTAATATTG CTGATTGTTT CAAGTTAATA CGTAACAAAT TGACAAATCA AATAAGTGAT 
CAACCATCAG GCATTAGAAG ATGTACATCT GCCACAAAGA CCCATACTGC AGGATATTTT 
ATACTATATG GACAGAATAT TTACGGTTAT ATTCTTCTTG GAAATGTTAA TCAAGTGGTT 
GGCGCTCGGC TTCAAAGTGT ACTTCACCAA CGCGTGGTGT TGGCTCGATT TCGTGATTGT 
CATGGTATCG CTTATCAACT TCGTTGCTTC ACTTGTTGGA GCTGGTGGTA TTCAAGCCTT 
CAAGACTATG CGAACGTTAA GAGCACTGAG ACCACTACGT GCCATGTCCC GTATGCAGGG 
CATGAGGGTC GTCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188642.1
CDS	269..3646
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTDYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGIRRCTSATKTHTAGYFILYGQNIYGYILLGNVNQVVGARLQSVLHQRVVLARFRDCHGIAYQLRCFTCWSWWYSSLQDYANVKSTETTTCHVPYAGHEGRR

Target ORF information:

RefSeq Version	NM_001201713.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AU, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201713.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GATTATGTGA CCATGGGTAT AGATTTAGGT 
AATCTAGCAG CCCTGCGAAC GTTTAGGGTG CTGCGAGCGC TTAAAACCGT AGCCATTGTG 
CCAGGCTTGA AGACCATCGT CGGCGCCGTC ATCGAATCGG TGAAGAATCT GCGCGATGTG 
ATTATCCTGA CCATGTTCTC CCTGTCGGTG TTCGCGTTGA TGGGCCTACA GATCTATATG 
GGCGTGCTCA CGCAGAAGTG CATCAAGAAG TTCCCGCTGG ACGGTTCCTG GGGCAATCTG 
ACCGACGAGA ACTGGGACTA TCACAATCGC AATAGCTCCA ATTGGTATTC CGAGGACGAG 
GGCATCTCAT TTCCGTTATG CGGCAATATA TCCGGTGCGG GGCAATGCGA CGACGATTAC 
GTGTGCCTGC AGGGGTTTGG TCCGAATCCG AATTATGGCT ACACCAGCTT CGATTCGTTC 
GGATGGGCTT TCCTGTCCGC CTTCCGGCTG ATGACACAGG ACTTCTGGGA GGATCTGTAC 
CAGCTGGTGT TGCGCGCCGC CGGACCATGG CACATGCTGT TCTTTATAGT CATCATCTTC 
CTAGGTTCAT TCTATCTTGT GAATTTGATT TTGGCCATTG TTGCCATGTC GTATGACGAA 
TTGCAAAAGA AGGCCGAAGA AGAAGAGGCT GCCGAAGAGG AGGCGATACG TGAAGCGGAA 
GAAGCTGCCG CCGCCAAAGC GGCCAAGCTG GAGGAGCGGG CCAATGCGCA GGCTCAGGCA 
GCAGCGGATG CGGCTGCCGC CGAAGAGGCT GCACTGCATC CGGAAATGGC CAAGAGTCCG 
ACGTATTCTT GCATCAGCTA TGAGCTATTT GTTGGCGGCG AGAAGGGCAA CGATGACAAC 
AACAAAGAGA AGATGTCCAT TCGGAGCGTC GAGGTGGAGT CGGAGTCGGT GAGCGTTATA 
CAAAGACAAC CAGCACCTAC CACAGCACAC CAAGCTACCA AAGTTCGTAA AGTGAGCACG 
TACACGATAC GGAACGGACG TGGCCGCTTT GGTATACCCG GTAGCGATCG TAAGCCATTG 
GTATTGTCAA CATATCAGGA TGCCCAGCAG CACTTGCCCT ATGCCGACGA CTCGAATGCC 
GTCACCCCGA TGTCCGAAGA GAATGGGGCC ATCATAGTGC CCGTGTACTA TGGCAATCTA 
GGCTCCCGAC ACTCATCGTA TACCTCGCAT CAGTCCCGAA TATCGTATAC CTCACATGGC 
GATCTACTCG GCGGCATGGC CGTCATGGGC GTCAGCACAA TGACCAAGGA GAGCAAATTG 
CGCAACCGGA ACACACGCAA TCAATCAGTG GGCGCCACCA ATGGCGGCAC CACCTGTCTG 
GACACCAATC ACAAGCTCGA TCATCGCGAC TACGAAATTG GCCTGGAGTG CACGGACGAA 
GCTGGCAAGA TTAAACATCA TGACAATCCT TTTATCGAGC CCGTCCAGAC ACAAACGGTG 
GTTGATATGA AAGATGTGAT GGTCCTGAAT GACATCATCG AACAGGCCGC TGGTCGGCAC 
AGTCGGGCAA GCGATCGCGG TGTCTCCGTT TACTATTTCC CAACAGAGGA CGATGACGAG 
GATGGGCCGA CGTTCAAAGA CAAGGCACTC GAAGTGATCC TCAAAGGCAT CGATGTGTTT 
TGTGTGTGGG ACTGTTGCTG GGTTTGGTTG AAATTTCAGG AGTGGGTATC GCTCATCGTC 
TTCGATCCCT TCGTCGAGCT CTTCATCACG CTGTGCATTG TGGTCAACAC GATGTTCATG 
GCAATGGATC ACCACGATAT GAACAAGGAG ATGGAACGCG TGCTCAAGAG TGGCAACTAT 
TTCTTCACCG CCACCTTTGC CATCGAGGCC ACCATGAAGC TAATGGCCAT GAGCCCCAAG 
TACTATTTCC AGGAGGGCTG GAACATCTTC GACTTCATTA TCGTGGCCCT ATCGCTATTG 
GAACTGGGAC TCGAGGGTGT CCAGGGTCTG TCCGTATTGC GTTCCTTTCG ATTGCTGCGT 
GTATTCAAAC TGGCCAAGTC TTGGCCCACA CTTAATTTAC TCATTTCGAT TATGGGACGC 
ACCATGGGCG CTTTGGGTAA TCTGACATTT GTACTTTGCA TTATCATCTT CATCTTTGCG 
GTGATGGGAA TGCAACTGTT CGGAAAGAAT TATCATGATC ACAAGGACCG CTTTCCGGAT 
GGCGACCTGC CGCGCTGGAA CTTCACCGAC TTTATGCACA GCTTCATGAT CGTGTTCCGG 
GTGCTCTGCG GAGAATGGAT CGAGTCCATG TGGGACTGCA TGTACGTGGG CGATGTCTCG 
TGCATTCCCT TCTTCTTGGC CACCGTTGTC ATCGGCAATC TTGTGGTACT TAACCTTTTC 
TTAGCCTTGC TTTTGTCCAA TTTTGGCTCA TCTAGCTTAT CAGCGCCGAC TGCCGATAAC 
GATACGAATA AAATAGCCGA GGCCTTCAAT CGAATTGGCC GATTTAAAAG TTGGGTTAAG 
CGTAATATTG CTGATTGTTT CAAGTTAATA CGTAACAAAT TGACAAATCA AATAAGTGAT 
CAACCATCAG GCATTAGAAG ATGTACATCT GCCACAAAGA CCCATACTGC AGGATATTTT 
ATACTATATG GACAGAATAT TTACGGTTAT ATTCTTCTTG GAAATGTTAA TCAAGTGGTT 
GGCGCTCGGC TTCAAAGTGT ACTTCACCAA CGCGTGGTGT TGGCTCGATT TCGTGATTGT 
CATGGTATCG CTTATCAACT TCGTTGCTTC ACTTGTTGGA GCTGGTGGTA TTCAAGCCTT 
CAAGACTATG CGAACGTTAA GAGCACTGAG ACCACTACGT GCCATGTCCC GTATGCAGGG 
CATGAGGGTC GTCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001144733.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138205.1 Drosophila melanogaster paralytic (para), transcript variant D, mRNA.

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CloneID	OFa01805
Clone ID Related Accession (Same CDS sequence)	NM_001144733.2
Accession Version	NM_001144733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_001144733.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGAGGACG ATGACGAGGA TGGGCCGACG 
TTCAAAGACA AGGCACTCGA AGTGATCCTC AAAGGCATCG ATGTGTTTTG TGTGTGGGAC 
TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG TGGGTATCGC TCATCGTCTT CGATCCCTTC 
GTCGAGCTCT TCATCACGCT GTGCATTGTG GTCAACACGA TGTTCATGGC AATGGATCAC 
CACGATATGA ACAAGGAGAT GGAACGCGTG CTCAAGAGTG GCAACTATTT CTTCACCGCC 
ACCTTTGCCA TCGAGGCCAC CATGAAGCTA ATGGCCATGA GCCCCAAGTA CTATTTCCAG 
GAGGGCTGGA ACATCTTCGA CTTCATTATC GTGGCCCTAT CGCTATTGGA ACTGGGACTC 
GAGGGTGTCC AGGGTCTGTC CGTATTGCGT TCCTTTCGAT TGCTGCGTGT TTTCAAGCTG 
GCCAAATCGT GGCCGACGCT GAATTTGCTC ATATCGATTA TGGGCCGCAC TGTCGGCGCC 
CTCGGCAATC TGACCTTCGT CCTGTGCATT ATTATCTTCA TATTCGCGGT CATGGGCATG 
CAGCTGTTTG GCAAGAACTA CACAGATCAC AAGGACCGCT TTCCGGATGG CGACCTGCCG 
CGCTGGAACT TCACCGACTT TATGCACAGC TTCATGATCG TGTTCCGGGT GCTCTGCGGA 
GAATGGATCG AGTCCATGTG GGACTGCATG TACGTGGGCG ATGTCTCGTG CATTCCCTTC 
TTCTTGGCCA CCGTTGTCAT CGGCAATCTT GTGGTACTTA ACCTTTTCTT AGCCTTGCTT 
TTGTCCAATT TTGGCTCATC TAGCTTATCA GCGCCGACTG CCGATAACGA TACGAATAAA 
ATAGCCGAGG CCTTCAATCG AATTGGCCGA TTTAAAAGTT GGGTTAAGCG TAATATTGCT 
GATTGTTTCA AGTTAATACG TAACAAATTG ACAAATCAAA TAAGTGATCA ACCATCAGAG 
CATGGTGACA ACGAACTGGA GCTGGGCCAC GACGAGATCC TCGCCGACGG CCTCATCAAG 
AAGGGGATCA AGGAGCAGAC GCAACTGGAG GTGGCCATCG GGGATGGCAT GGAATTCACG 
ATACACGGCG ACATGAAGAA CAACAAGCCG AAGAAATCCA AATATCTAAA TAACGCAACG 
ATGATTGGCA ACTCAATTAA CCACCAAGAC AATAGACTGG AACACGAGCT AAACCATAGA 
GGTTTGTCCT TACAGGACGA CGACACTGCC AGCATTAACT CATATGGTAG CCATAAGAAT 
CGACCATTCA AGGACGAGAG CCACAAGGGC AGCGCCGAGA CGATGGAGGG CGAGGAGAAG 
CGCGACGCCA GCAAGGAGGA TTTAGGTCTC GACGAGGAAC TGGACGAGGA GGGCGAATGC 
GAGGAGGGCC CGCTCGACGG TGATATCATT ATTCATGCAC ACGACGAGGA TATACTCGAT 
GAATATCCAG CTGATTGCTG CCCCGATTCG TACTATAAGA AATTTCCGAT CTTAGCCGGT 
GACGATGACT CGCCGTTCTG GCAAGGATGG GGCAATTTAC GACTGAAAAC TTTTCAATTA 
ATTGAAAATA AATATTTTGA AACAGCTGTT ATCACTATGA TTTTAATGAG TAGCTTAGCT 
TTGGCATTAG AAGATGTACA TCTGCCACAA AGACCCATAC TGCAGGATAT TTTATACTAT 
ATGGACAGAA TATTTACGGT TATATTCTTC TTGGAAATGT TAATCAAGTG GTTGGCGCTC 
GGCTTCAAAG TGTACTTCAC CAACGCGTGG TGTTGGCTCG ATTTCGTGAT TGTCATGCTG 
TCGCTAATTA ATTTGGCCGC GGTCTGGTCC GGGGCCGATG ATGTGCCCGC CTTTCGCTCG 
ATGCGAACTC TGCGCGCCCT GCGACCACTG CGGGCAGTTT CGCGCTGGGA GGGCATGAAA 
GTCGTCGTTA ATGCGCTGGT ACAAGCTATA CCGTCCATCT TCAATGTGCT ATTGGTGTGT 
CTAATATTTT GGCTAATTTT TGCCATAATG GGTGTACAGC TTTTTGCTGG AAAATATTTT 
AAGTGCGAGG ACATGAATGG CACGAAGCTC AGCCACGAGA TCATACCAAA TCGCAATGCC 
TGCGAGAGCG AGAACTACAC GTGGGTGAAT TCAGCAATGA ATTTCGATCA TGTAGGTAAC 
GCGTATCTGT GCCTTTTCCA AGTGGCCACC TTCAAAGGCT GGATACAAAT CATGAACGAT 
GCTATCGATT CACGAGAGGT GGACAAGCAA CCAATTCGTG AAACGAACAT CTACATGTAT 
TTATATTTCG TATTCTTCAT CATATTTGGA TCATTTTTCA CACTCAATCT GTTCATTGGT 
GTTATCATTG ATAATTTTAA TGAGCAAAAG AAAAAAGCAG GTGGATCATT AGAAATGTTC 
ATGACAGAAG ATCAGAAAAA GTACTATAAT GCTATGAAAA AGATGGGCTC TAAAAAACCA 
TTAAAAGCCA TTCCAAGACC AAGGTGGCGA CCACAAGCAA TAGTCTTTGA AATAGTAACC 
GATAAGAAAT TCGATATAAT CATTATGTTA TTCATTGGTC TGAACATGTT CACCATGACC 
CTCGATCGTT ACGATGCGTC GGACACGTAT AACGCGGTCC TAGACTATCT CAATGCGATA 
TTCGTAGTTA TTTTCAGTTC CGAATGTCTA TTAAAAATAT TCGCTTTACG ATATCACTAT 
TTTATTGAGC CATGGAATTT ATTTGATGTA GTAGTTGTCA TTTTATCCAT CTTAGGTCTT 
GTACTTAGCG ATATTATCGA GAAGTACTTC GTGTCGCCGA CCCTGCTCCG AGTGGTGCGT 
GTGGCGAAAG TGGGCCGTGT CCTTCGACTG GTGAAGGGAG CCAAGGGCAT TCGGACACTG 
CTCTTCGCGT TGGCCATGTC GCTGCCGGCC CTGTTCAACA TCTGCCTGCT GCTGTTCCTG 
GTCATGTTCA TCTTTGCCAT TTTCGGCATG TCGTTCTTCA TGCACGTGAA GGAGAAGAGC 
GGCATCAACG ACGTCTACAA CTTCAAGACC TTTGGCCAGA GCATGATCCT GCTCTTTCAG 
ATGTCGACGT CAGCCGGTTG GGATGGTGTA CTGGACGCCA TTATCAATGA GGAAGCATGC 
GATCCACCCG ACAACGACAA AGGCTATCCG GGCAATTGTG GTTCAGCGAC CGTTGGAATA 
ACGTTTCTCC TCTCATACCT AGTTATAAGC TTTTTGATAG TTATTAATAT GTACATTGCT 
GTCATTCTCG AGAACTATAG TCAGGCCACC GAGGACGTGC AAGAGGGTCT AACCGACGAC 
GACTACGACA TGTACTATGA GATCTGGCAG CAATTCGATC CGGAGGGCAC CCAGTACATA 
CGCTATGATC AGCTGTCCGA ATTCCTGGAC GTACTGGAGC CCCCGCTGCA GATCCACAAA 
CCGAACAAGT ACAAGATCAT ATCGATGGAC ATACCCATCT GTCGCGGTGA CCTCATGTAC 
TGCGTCGACA TCCTCGACGC CCTTACGAAA GACTTCTTTG CGCGGAAGGG CAATCCGATA 
GAGGAGACGG GTGAGATTGG TGAGATAGCG GCCCGCCCGG ATACGGAGGG CTACGAGCCC 
GTCTCATCAA CGCTGTGGCG TCAGCGTGAG GAGTACTGCG CCCGGCTAAT CCAGCACGCC 
TGGCGAAAGC ACAAGGCGCG CGGCGAGGGA GGTGGGTCCT TTGAGCCGGA TACGGATCAT 
GGCGATGGCG GTGATCCGGA TGCCGGGGAC CCGGCGCCCG ATGAAGCAAC GGACGGCGAT 
GCGCCCGCTG GTGGAGATGG TAGTGTTAAC GGTACTGCAG AAGGAGCTGC CGATGCCGAT 
GAGAGTAATG TAAATAGTCC GGGTGAGGAT GCAGCGGCGG CGGCAGCAGC AGCAGCAGCA 
GCGGCGGCGG CGGGCACGAC GACGGCGGGA AGTCCCGGAG CGGGTAGCGC CGGGCGACAG 
ACCGCCGTTC TCGTGGAGAG CGACGGGTTC GTGACGAAGA ACGGCCACAA GGTGGTCATC 
CACTCGCGAT CGCCGAGCAT CACGTCGCGC ACGGCGGATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138205.1
CDS	269..6613
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATMIGNSINHQDNRLEHELNHRGLSLQDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001144733.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144733.2

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGAGGACG ATGACGAGGA TGGGCCGACG 
TTCAAAGACA AGGCACTCGA AGTGATCCTC AAAGGCATCG ATGTGTTTTG TGTGTGGGAC 
TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG TGGGTATCGC TCATCGTCTT CGATCCCTTC 
GTCGAGCTCT TCATCACGCT GTGCATTGTG GTCAACACGA TGTTCATGGC AATGGATCAC 
CACGATATGA ACAAGGAGAT GGAACGCGTG CTCAAGAGTG GCAACTATTT CTTCACCGCC 
ACCTTTGCCA TCGAGGCCAC CATGAAGCTA ATGGCCATGA GCCCCAAGTA CTATTTCCAG 
GAGGGCTGGA ACATCTTCGA CTTCATTATC GTGGCCCTAT CGCTATTGGA ACTGGGACTC 
GAGGGTGTCC AGGGTCTGTC CGTATTGCGT TCCTTTCGAT TGCTGCGTGT TTTCAAGCTG 
GCCAAATCGT GGCCGACGCT GAATTTGCTC ATATCGATTA TGGGCCGCAC TGTCGGCGCC 
CTCGGCAATC TGACCTTCGT CCTGTGCATT ATTATCTTCA TATTCGCGGT CATGGGCATG 
CAGCTGTTTG GCAAGAACTA CACAGATCAC AAGGACCGCT TTCCGGATGG CGACCTGCCG 
CGCTGGAACT TCACCGACTT TATGCACAGC TTCATGATCG TGTTCCGGGT GCTCTGCGGA 
GAATGGATCG AGTCCATGTG GGACTGCATG TACGTGGGCG ATGTCTCGTG CATTCCCTTC 
TTCTTGGCCA CCGTTGTCAT CGGCAATCTT GTGGTACTTA ACCTTTTCTT AGCCTTGCTT 
TTGTCCAATT TTGGCTCATC TAGCTTATCA GCGCCGACTG CCGATAACGA TACGAATAAA 
ATAGCCGAGG CCTTCAATCG AATTGGCCGA TTTAAAAGTT GGGTTAAGCG TAATATTGCT 
GATTGTTTCA AGTTAATACG TAACAAATTG ACAAATCAAA TAAGTGATCA ACCATCAGAG 
CATGGTGACA ACGAACTGGA GCTGGGCCAC GACGAGATCC TCGCCGACGG CCTCATCAAG 
AAGGGGATCA AGGAGCAGAC GCAACTGGAG GTGGCCATCG GGGATGGCAT GGAATTCACG 
ATACACGGCG ACATGAAGAA CAACAAGCCG AAGAAATCCA AATATCTAAA TAACGCAACG 
ATGATTGGCA ACTCAATTAA CCACCAAGAC AATAGACTGG AACACGAGCT AAACCATAGA 
GGTTTGTCCT TACAGGACGA CGACACTGCC AGCATTAACT CATATGGTAG CCATAAGAAT 
CGACCATTCA AGGACGAGAG CCACAAGGGC AGCGCCGAGA CGATGGAGGG CGAGGAGAAG 
CGCGACGCCA GCAAGGAGGA TTTAGGTCTC GACGAGGAAC TGGACGAGGA GGGCGAATGC 
GAGGAGGGCC CGCTCGACGG TGATATCATT ATTCATGCAC ACGACGAGGA TATACTCGAT 
GAATATCCAG CTGATTGCTG CCCCGATTCG TACTATAAGA AATTTCCGAT CTTAGCCGGT 
GACGATGACT CGCCGTTCTG GCAAGGATGG GGCAATTTAC GACTGAAAAC TTTTCAATTA 
ATTGAAAATA AATATTTTGA AACAGCTGTT ATCACTATGA TTTTAATGAG TAGCTTAGCT 
TTGGCATTAG AAGATGTACA TCTGCCACAA AGACCCATAC TGCAGGATAT TTTATACTAT 
ATGGACAGAA TATTTACGGT TATATTCTTC TTGGAAATGT TAATCAAGTG GTTGGCGCTC 
GGCTTCAAAG TGTACTTCAC CAACGCGTGG TGTTGGCTCG ATTTCGTGAT TGTCATGCTG 
TCGCTAATTA ATTTGGCCGC GGTCTGGTCC GGGGCCGATG ATGTGCCCGC CTTTCGCTCG 
ATGCGAACTC TGCGCGCCCT GCGACCACTG CGGGCAGTTT CGCGCTGGGA GGGCATGAAA 
GTCGTCGTTA ATGCGCTGGT ACAAGCTATA CCGTCCATCT TCAATGTGCT ATTGGTGTGT 
CTAATATTTT GGCTAATTTT TGCCATAATG GGTGTACAGC TTTTTGCTGG AAAATATTTT 
AAGTGCGAGG ACATGAATGG CACGAAGCTC AGCCACGAGA TCATACCAAA TCGCAATGCC 
TGCGAGAGCG AGAACTACAC GTGGGTGAAT TCAGCAATGA ATTTCGATCA TGTAGGTAAC 
GCGTATCTGT GCCTTTTCCA AGTGGCCACC TTCAAAGGCT GGATACAAAT CATGAACGAT 
GCTATCGATT CACGAGAGGT GGACAAGCAA CCAATTCGTG AAACGAACAT CTACATGTAT 
TTATATTTCG TATTCTTCAT CATATTTGGA TCATTTTTCA CACTCAATCT GTTCATTGGT 
GTTATCATTG ATAATTTTAA TGAGCAAAAG AAAAAAGCAG GTGGATCATT AGAAATGTTC 
ATGACAGAAG ATCAGAAAAA GTACTATAAT GCTATGAAAA AGATGGGCTC TAAAAAACCA 
TTAAAAGCCA TTCCAAGACC AAGGTGGCGA CCACAAGCAA TAGTCTTTGA AATAGTAACC 
GATAAGAAAT TCGATATAAT CATTATGTTA TTCATTGGTC TGAACATGTT CACCATGACC 
CTCGATCGTT ACGATGCGTC GGACACGTAT AACGCGGTCC TAGACTATCT CAATGCGATA 
TTCGTAGTTA TTTTCAGTTC CGAATGTCTA TTAAAAATAT TCGCTTTACG ATATCACTAT 
TTTATTGAGC CATGGAATTT ATTTGATGTA GTAGTTGTCA TTTTATCCAT CTTAGGTCTT 
GTACTTAGCG ATATTATCGA GAAGTACTTC GTGTCGCCGA CCCTGCTCCG AGTGGTGCGT 
GTGGCGAAAG TGGGCCGTGT CCTTCGACTG GTGAAGGGAG CCAAGGGCAT TCGGACACTG 
CTCTTCGCGT TGGCCATGTC GCTGCCGGCC CTGTTCAACA TCTGCCTGCT GCTGTTCCTG 
GTCATGTTCA TCTTTGCCAT TTTCGGCATG TCGTTCTTCA TGCACGTGAA GGAGAAGAGC 
GGCATCAACG ACGTCTACAA CTTCAAGACC TTTGGCCAGA GCATGATCCT GCTCTTTCAG 
ATGTCGACGT CAGCCGGTTG GGATGGTGTA CTGGACGCCA TTATCAATGA GGAAGCATGC 
GATCCACCCG ACAACGACAA AGGCTATCCG GGCAATTGTG GTTCAGCGAC CGTTGGAATA 
ACGTTTCTCC TCTCATACCT AGTTATAAGC TTTTTGATAG TTATTAATAT GTACATTGCT 
GTCATTCTCG AGAACTATAG TCAGGCCACC GAGGACGTGC AAGAGGGTCT AACCGACGAC 
GACTACGACA TGTACTATGA GATCTGGCAG CAATTCGATC CGGAGGGCAC CCAGTACATA 
CGCTATGATC AGCTGTCCGA ATTCCTGGAC GTACTGGAGC CCCCGCTGCA GATCCACAAA 
CCGAACAAGT ACAAGATCAT ATCGATGGAC ATACCCATCT GTCGCGGTGA CCTCATGTAC 
TGCGTCGACA TCCTCGACGC CCTTACGAAA GACTTCTTTG CGCGGAAGGG CAATCCGATA 
GAGGAGACGG GTGAGATTGG TGAGATAGCG GCCCGCCCGG ATACGGAGGG CTACGAGCCC 
GTCTCATCAA CGCTGTGGCG TCAGCGTGAG GAGTACTGCG CCCGGCTAAT CCAGCACGCC 
TGGCGAAAGC ACAAGGCGCG CGGCGAGGGA GGTGGGTCCT TTGAGCCGGA TACGGATCAT 
GGCGATGGCG GTGATCCGGA TGCCGGGGAC CCGGCGCCCG ATGAAGCAAC GGACGGCGAT 
GCGCCCGCTG GTGGAGATGG TAGTGTTAAC GGTACTGCAG AAGGAGCTGC CGATGCCGAT 
GAGAGTAATG TAAATAGTCC GGGTGAGGAT GCAGCGGCGG CGGCAGCAGC AGCAGCAGCA 
GCGGCGGCGG CGGGCACGAC GACGGCGGGA AGTCCCGGAG CGGGTAGCGC CGGGCGACAG 
ACCGCCGTTC TCGTGGAGAG CGACGGGTTC GTGACGAAGA ACGGCCACAA GGTGGTCATC 
CACTCGCGAT CGCCGAGCAT CACGTCGCGC ACGGCGGATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201689.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188618.1 Drosophila melanogaster paralytic (para), transcript variant W, mRNA.

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CloneID	OFa01806
Clone ID Related Accession (Same CDS sequence)	NM_001201689.1
Accession Version	NM_001201689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform W
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGAGGACG ATGACGAGGA TGGGCCGACG TTCAAAGACA AGGCACTCGA AGTGATCCTC 
AAAGGCATCG ATGTGTTTTG TGTGTGGGAC TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG 
TGGGTATCGC TCATCGTCTT CGATCCCTTC GTCGAGCTCT TCATCACGCT GTGCATTGTG 
GTCAACACGA TGTTCATGGC AATGGATCAC CACGATATGA ACAAGGAGAT GGAACGCGTG 
CTCAAGAGTG GCAACTATTT CTTCACCGCC ACCTTTGCCA TCGAGGCCAC CATGAAGCTA 
ATGGCCATGA GCCCCAAGTA CTATTTCCAG GAGGGCTGGA ACATCTTCGA CTTCATTATC 
GTGGCCCTAT CGCTATTGGA ACTGGGACTC GAGGGTGTCC AGGGTCTGTC CGTATTGCGT 
TCCTTTCGAT TGCTGCGTGT ATTCAAACTG GCCAAGTCTT GGCCCACACT TAATTTACTC 
ATTTCGATTA TGGGACGCAC CATGGGCGCT TTGGGTAATC TGACATTTGT ACTTTGCATT 
ATCATCTTCA TCTTTGCGGT GATGGGAATG CAACTGTTCG GAAAGAATTA TCATGATCAC 
AAGGACCGCT TTCCGGATGG CGACCTGCCG CGCTGGAACT TCACCGACTT TATGCACAGC 
TTCATGATCG TGTTCCGGGT GCTCTGCGGA GAATGGATCG AGTCCATGTG GGACTGCATG 
TACGTGGGCG ATGTCTCGTG CATTCCCTTC TTCTTGGCCA CCGTTGTCAT CGGCAATCTT 
GTGGTACTTA ACCTTTTCTT AGCCTTGCTT TTGTCCAATT TTGGCTCATC TAGCTTATCA 
GCGCCGACTG CCGATAACGA TACGAATAAA ATAGCCGAGG CCTTCAATCG AATTGGCCGA 
TTTAAAAGTT GGGTTAAGCG TAATATTGCT GATTGTTTCA AGTTAATACG TAACAAATTG 
ACAAATCAAA TAAGTGATCA ACCATCAGGT GAGAGGACCA ACCAGATCAG TTGGATTTGG 
AGCGAAGGCA AAGGCGTTTG TCGTTGTATA TCTGCAGAGC ATGGTGACAA CGAACTGGAG 
CTGGGCCACG ACGAGATCCT CGCCGACGGC CTCATCAAGA AGGGGATCAA GGAGCAGACG 
CAACTGGAGG TGGCCATCGG GGATGGCATG GAATTCACGA TACACGGCGA CATGAAGAAC 
AACAAGCCGA AGAAATCCAA ATATCTAAAT AACGCAACGG ACGACGACAC TGCCAGCATT 
AACTCATATG GTAGCCATAA GAATCGACCA TTCAAGGACG AGAGCCACAA GGGCAGCGCC 
GAGACGATGG AGGGCGAGGA GAAGCGCGAC GCCAGCAAGG AGGATTTAGG TCTCGACGAG 
GAACTGGACG AGGAGGGCGA ATGCGAGGAG GGCCCGCTCG ACGGTGATAT CATTATTCAT 
GCACACGACG AGGATATACT CGATGAATAT CCAGCTGATT GCTGCCCCGA TTCGTACTAT 
AAGAAATTTC CGATCTTAGC CGGTGACGAT GACTCGCCGT TCTGGCAAGG ATGGGGCAAT 
TTACGACTGA AAACTTTTCA ATTAATTGAA AATAAATATT TTGAAACAGC TGTTATCACT 
ATGATTTTAA TGAGTAGCTT AGCTTTGGCA TTAGAAGATG TACATCTGCC ACAAAGACCC 
ATACTGCAGG ATATTTTATA CTATATGGAC AGAATATTTA CGGTTATATT CTTCTTGGAA 
ATGTTAATCA AGTGGTTGGC GCTCGGCTTC AAAGTGTACT TCACCAACGC GTGGTGTTGG 
CTCGATTTCG TGATTGTCAT GGTATCGCTT ATCAACTTCG TTGCTTCACT TGTTGGAGCT 
GGTGGTATTC AAGCCTTCAA GACTATGCGA ACGTTAAGAG CACTGAGACC ACTACGTGCC 
ATGTCCCGTA TGCAGGGCAT GAGGGTCGTC GTTAATGCGC TGGTACAAGC TATACCGTCC 
ATCTTCAATG TGCTATTGGT GTGTCTAATA TTTTGGCTAA TTTTTGCCAT AATGGGTGTA 
CAGCTTTTTG CTGGAAAATA TTTTAAGTGC GAGGACATGA ATGGCACGAA GCTCAGCCAC 
GAGATCATAC CAAATCGCAA TGCCTGCGAG AGCGAGAACT ACACGTGGGT GAATTCAGCA 
ATGAATTTCG ATCATGTAGG TAACGCGTAT CTGTGCCTTT TCCAAGTGGC CACCTTCAAA 
GGCTGGATAC AAATCATGAA CGATGCTATC GATTCACGAG AGGTGGACAA GCAACCAATT 
CGTGAAACGA ACATCTACAT GTATTTATAT TTCGTATTCT TCATCATATT TGGATCATTT 
TTCACACTCA ATCTGTTCAT TGGTGTTATC ATTGATAATT TTAATGAGCA AAAGAAAAAA 
GCAGGTGGAT CATTAGAAAT GTTCATGACA GAAGATCAGA AAAAGTACTA TAATGCTATG 
AAAAAGATGG GCTCTAAAAA ACCATTAAAA GCCATTCCAA GACCAAGGTG GCGACCACAA 
GCAATAGTCT TTGAAATAGT AACCGATAAG AAATTCGATA TAATCATTAT GTTATTCATT 
GGTCTGAACA TGTTCACCAT GACCCTCGAT CGTTACGATG CGTCGGACAC GTATAACGCG 
GTCCTAGACT ATCTCAATGC GATATTCGTA GTTATTTTCA GTTCCGAATG TCTATTAAAA 
ATATTCGCTT TACGATATCA CTATTTTATT GAGCCATGGA ATTTATTTGA TGTAGTAGTT 
GTCATTTTAT CCATCTTAGG TCTTGTACTT AGCGATATTA TCGAGAAGTA CTTCGTGTCG 
CCGACCCTGC TCCGAGTGGT GCGTGTGGCG AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG 
GGAGCCAAGG GCATTCGGAC ACTGCTCTTC GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC 
AACATCTGCC TGCTGCTGTT CCTGGTCATG TTCATCTTTG CCATTTTCGG CATGTCGTTC 
TTCATGCACG TGAAGGAGAA GAGCGGCATC AACGACGTCT ACAACTTCAA GACCTTTGGC 
CAGAGCATGA TCCTGCTCTT TCAGATGTCG ACGTCAGCCG GTTGGGATGG TGTACTGGAC 
GCCATTATCA ATGAGGAAGC ATGCGATCCA CCCGACAACG ACAAAGGCTA TCCGGGCAAT 
TGTGGTTCAG CGACCGTTGG AATAACGTTT CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG 
ATAGTTATTA ATATGTACAT TGCTGTCATT CTCGAGAACT ATAGTCAGGC CACCGAGGAC 
GTGCAAGAGG GTCTAACCGA CGACGACTAC GACATGTACT ATGAGATCTG GCAGCAATTC 
GATCCGGAGG GCACCCAGTA CATACGCTAT GATCAGCTGT CCGAATTCCT GGACGTACTG 
GAGCCCCCGC TGCAGATCCA CAAACCGAAC AAGTACAAGA TCATATCGAT GGACATACCC 
ATCTGTCGCG GTGACCTCAT GTACTGCGTC GACATCCTCG ACGCCCTTAC GAAAGACTTC 
TTTGCGCGGA AGGGCAATCC GATAGAGGAG ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC 
CCGGATACGG AGGGCTACGA GCCCGTCTCA TCAACGCTGT GGCGTCAGCG TGAGGAGTAC 
TGCGCCCGGC TAATCCAGCA CGCCTGGCGA AAGCACAAGG CGCGCGGCGA GGGAGGTGGG 
TCCTTTGAGC CGGATACGGA TCATGGCGAT GGCGGTGATC CGGATGCCGG GGACCCGGCG 
CCCGATGAAG CAACGGACGG CGATGCGCCC GCTGGTGGAG ATGGTAGTGT TAACGGTACT 
GCAGAAGGAG CTGCCGATGC CGATGAGAGT AATGTAAATA GTCCGGGTGA GGATGCAGCG 
GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG GCGGCGGGCA CGACGACGGC GGGAAGTCCC 
GGAGCGGGTA GCGCCGGGCG ACAGACCGCC GTTCTCGTGG AGAGCGACGG GTTCGTGACG 
AAGAACGGCC ACAAGGTGGT CATCCACTCG CGATCGCCGA GCATCACGTC GCGCACGGCG 
GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188618.1
CDS	269..6577
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201689.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant W, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201689.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGAGGACG ATGACGAGGA TGGGCCGACG TTCAAAGACA AGGCACTCGA AGTGATCCTC 
AAAGGCATCG ATGTGTTTTG TGTGTGGGAC TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG 
TGGGTATCGC TCATCGTCTT CGATCCCTTC GTCGAGCTCT TCATCACGCT GTGCATTGTG 
GTCAACACGA TGTTCATGGC AATGGATCAC CACGATATGA ACAAGGAGAT GGAACGCGTG 
CTCAAGAGTG GCAACTATTT CTTCACCGCC ACCTTTGCCA TCGAGGCCAC CATGAAGCTA 
ATGGCCATGA GCCCCAAGTA CTATTTCCAG GAGGGCTGGA ACATCTTCGA CTTCATTATC 
GTGGCCCTAT CGCTATTGGA ACTGGGACTC GAGGGTGTCC AGGGTCTGTC CGTATTGCGT 
TCCTTTCGAT TGCTGCGTGT ATTCAAACTG GCCAAGTCTT GGCCCACACT TAATTTACTC 
ATTTCGATTA TGGGACGCAC CATGGGCGCT TTGGGTAATC TGACATTTGT ACTTTGCATT 
ATCATCTTCA TCTTTGCGGT GATGGGAATG CAACTGTTCG GAAAGAATTA TCATGATCAC 
AAGGACCGCT TTCCGGATGG CGACCTGCCG CGCTGGAACT TCACCGACTT TATGCACAGC 
TTCATGATCG TGTTCCGGGT GCTCTGCGGA GAATGGATCG AGTCCATGTG GGACTGCATG 
TACGTGGGCG ATGTCTCGTG CATTCCCTTC TTCTTGGCCA CCGTTGTCAT CGGCAATCTT 
GTGGTACTTA ACCTTTTCTT AGCCTTGCTT TTGTCCAATT TTGGCTCATC TAGCTTATCA 
GCGCCGACTG CCGATAACGA TACGAATAAA ATAGCCGAGG CCTTCAATCG AATTGGCCGA 
TTTAAAAGTT GGGTTAAGCG TAATATTGCT GATTGTTTCA AGTTAATACG TAACAAATTG 
ACAAATCAAA TAAGTGATCA ACCATCAGGT GAGAGGACCA ACCAGATCAG TTGGATTTGG 
AGCGAAGGCA AAGGCGTTTG TCGTTGTATA TCTGCAGAGC ATGGTGACAA CGAACTGGAG 
CTGGGCCACG ACGAGATCCT CGCCGACGGC CTCATCAAGA AGGGGATCAA GGAGCAGACG 
CAACTGGAGG TGGCCATCGG GGATGGCATG GAATTCACGA TACACGGCGA CATGAAGAAC 
AACAAGCCGA AGAAATCCAA ATATCTAAAT AACGCAACGG ACGACGACAC TGCCAGCATT 
AACTCATATG GTAGCCATAA GAATCGACCA TTCAAGGACG AGAGCCACAA GGGCAGCGCC 
GAGACGATGG AGGGCGAGGA GAAGCGCGAC GCCAGCAAGG AGGATTTAGG TCTCGACGAG 
GAACTGGACG AGGAGGGCGA ATGCGAGGAG GGCCCGCTCG ACGGTGATAT CATTATTCAT 
GCACACGACG AGGATATACT CGATGAATAT CCAGCTGATT GCTGCCCCGA TTCGTACTAT 
AAGAAATTTC CGATCTTAGC CGGTGACGAT GACTCGCCGT TCTGGCAAGG ATGGGGCAAT 
TTACGACTGA AAACTTTTCA ATTAATTGAA AATAAATATT TTGAAACAGC TGTTATCACT 
ATGATTTTAA TGAGTAGCTT AGCTTTGGCA TTAGAAGATG TACATCTGCC ACAAAGACCC 
ATACTGCAGG ATATTTTATA CTATATGGAC AGAATATTTA CGGTTATATT CTTCTTGGAA 
ATGTTAATCA AGTGGTTGGC GCTCGGCTTC AAAGTGTACT TCACCAACGC GTGGTGTTGG 
CTCGATTTCG TGATTGTCAT GGTATCGCTT ATCAACTTCG TTGCTTCACT TGTTGGAGCT 
GGTGGTATTC AAGCCTTCAA GACTATGCGA ACGTTAAGAG CACTGAGACC ACTACGTGCC 
ATGTCCCGTA TGCAGGGCAT GAGGGTCGTC GTTAATGCGC TGGTACAAGC TATACCGTCC 
ATCTTCAATG TGCTATTGGT GTGTCTAATA TTTTGGCTAA TTTTTGCCAT AATGGGTGTA 
CAGCTTTTTG CTGGAAAATA TTTTAAGTGC GAGGACATGA ATGGCACGAA GCTCAGCCAC 
GAGATCATAC CAAATCGCAA TGCCTGCGAG AGCGAGAACT ACACGTGGGT GAATTCAGCA 
ATGAATTTCG ATCATGTAGG TAACGCGTAT CTGTGCCTTT TCCAAGTGGC CACCTTCAAA 
GGCTGGATAC AAATCATGAA CGATGCTATC GATTCACGAG AGGTGGACAA GCAACCAATT 
CGTGAAACGA ACATCTACAT GTATTTATAT TTCGTATTCT TCATCATATT TGGATCATTT 
TTCACACTCA ATCTGTTCAT TGGTGTTATC ATTGATAATT TTAATGAGCA AAAGAAAAAA 
GCAGGTGGAT CATTAGAAAT GTTCATGACA GAAGATCAGA AAAAGTACTA TAATGCTATG 
AAAAAGATGG GCTCTAAAAA ACCATTAAAA GCCATTCCAA GACCAAGGTG GCGACCACAA 
GCAATAGTCT TTGAAATAGT AACCGATAAG AAATTCGATA TAATCATTAT GTTATTCATT 
GGTCTGAACA TGTTCACCAT GACCCTCGAT CGTTACGATG CGTCGGACAC GTATAACGCG 
GTCCTAGACT ATCTCAATGC GATATTCGTA GTTATTTTCA GTTCCGAATG TCTATTAAAA 
ATATTCGCTT TACGATATCA CTATTTTATT GAGCCATGGA ATTTATTTGA TGTAGTAGTT 
GTCATTTTAT CCATCTTAGG TCTTGTACTT AGCGATATTA TCGAGAAGTA CTTCGTGTCG 
CCGACCCTGC TCCGAGTGGT GCGTGTGGCG AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG 
GGAGCCAAGG GCATTCGGAC ACTGCTCTTC GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC 
AACATCTGCC TGCTGCTGTT CCTGGTCATG TTCATCTTTG CCATTTTCGG CATGTCGTTC 
TTCATGCACG TGAAGGAGAA GAGCGGCATC AACGACGTCT ACAACTTCAA GACCTTTGGC 
CAGAGCATGA TCCTGCTCTT TCAGATGTCG ACGTCAGCCG GTTGGGATGG TGTACTGGAC 
GCCATTATCA ATGAGGAAGC ATGCGATCCA CCCGACAACG ACAAAGGCTA TCCGGGCAAT 
TGTGGTTCAG CGACCGTTGG AATAACGTTT CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG 
ATAGTTATTA ATATGTACAT TGCTGTCATT CTCGAGAACT ATAGTCAGGC CACCGAGGAC 
GTGCAAGAGG GTCTAACCGA CGACGACTAC GACATGTACT ATGAGATCTG GCAGCAATTC 
GATCCGGAGG GCACCCAGTA CATACGCTAT GATCAGCTGT CCGAATTCCT GGACGTACTG 
GAGCCCCCGC TGCAGATCCA CAAACCGAAC AAGTACAAGA TCATATCGAT GGACATACCC 
ATCTGTCGCG GTGACCTCAT GTACTGCGTC GACATCCTCG ACGCCCTTAC GAAAGACTTC 
TTTGCGCGGA AGGGCAATCC GATAGAGGAG ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC 
CCGGATACGG AGGGCTACGA GCCCGTCTCA TCAACGCTGT GGCGTCAGCG TGAGGAGTAC 
TGCGCCCGGC TAATCCAGCA CGCCTGGCGA AAGCACAAGG CGCGCGGCGA GGGAGGTGGG 
TCCTTTGAGC CGGATACGGA TCATGGCGAT GGCGGTGATC CGGATGCCGG GGACCCGGCG 
CCCGATGAAG CAACGGACGG CGATGCGCCC GCTGGTGGAG ATGGTAGTGT TAACGGTACT 
GCAGAAGGAG CTGCCGATGC CGATGAGAGT AATGTAAATA GTCCGGGTGA GGATGCAGCG 
GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG GCGGCGGGCA CGACGACGGC GGGAAGTCCC 
GGAGCGGGTA GCGCCGGGCG ACAGACCGCC GTTCTCGTGG AGAGCGACGG GTTCGTGACG 
AAGAACGGCC ACAAGGTGGT CATCCACTCG CGATCGCCGA GCATCACGTC GCGCACGGCG 
GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201684.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188613.1 Drosophila melanogaster paralytic (para), transcript variant R, mRNA.

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CloneID	OFa01807
Clone ID Related Accession (Same CDS sequence)	NM_001201684.1
Accession Version	NM_001201684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform R
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGAGGACG ATGACGAGGA TGGGCCGACG TTCAAAGACA AGGCACTCGA AGTGATCCTC 
AAAGGCATCG ATGTGTTTTG TGTGTGGGAC TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG 
TGGGTATCGC TCATCGTCTT CGATCCCTTC GTCGAGCTCT TCATCACGCT GTGCATTGTG 
GTCAACACGA TGTTCATGGC AATGGATCAC CACGATATGA ACAAGGAGAT GGAACGCGTG 
CTCAAGAGTG GCAACTATTT CTTCACCGCC ACCTTTGCCA TCGAGGCCAC CATGAAGCTA 
ATGGCCATGA GCCCCAAGTA CTATTTCCAG GAGGGCTGGA ACATCTTCGA CTTCATTATC 
GTGGCCCTAT CGCTATTGGA ACTGGGACTC GAGGGTGTCC AGGGTCTGTC CGTATTGCGT 
TCCTTTCGAT TGCTGCGTGT TTTCAAGCTG GCCAAATCGT GGCCGACGCT GAATTTGCTC 
ATATCGATTA TGGGCCGCAC TGTCGGCGCC CTCGGCAATC TGACCTTCGT CCTGTGCATT 
ATTATCTTCA TATTCGCGGT CATGGGCATG CAGCTGTTTG GCAAGAACTA CACAGATCAC 
AAGGACCGCT TTCCGGATGG CGACCTGCCG CGCTGGAACT TCACCGACTT TATGCACAGC 
TTCATGATCG TGTTCCGGGT GCTCTGCGGA GAATGGATCG AGTCCATGTG GGACTGCATG 
TACGTGGGCG ATGTCTCGTG CATTCCCTTC TTCTTGGCCA CCGTTGTCAT CGGCAATCTT 
GTGGTACTTA ACCTTTTCTT AGCCTTGCTT TTGTCCAATT TTGGCTCATC TAGCTTATCA 
GCGCCGACTG CCGATAACGA TACGAATAAA ATAGCCGAGG CCTTCAATCG AATTGGCCGA 
TTTAAAAGTT GGGTTAAGCG TAATATTGCT GATTGTTTCA AGTTAATACG TAACAAATTG 
ACAAATCAAA TAAGTGATCA ACCATCAGAG CATGGTGACA ACGAACTGGA GCTGGGCCAC 
GACGAGATCC TCGCCGACGG CCTCATCAAG AAGGGGATCA AGGAGCAGAC GCAACTGGAG 
GTGGCCATCG GGGATGGCAT GGAATTCACG ATACACGGCG ACATGAAGAA CAACAAGCCG 
AAGAAATCCA AATATCTAAA TAACGCAACG GACGACGACA CTGCCAGCAT TAACTCATAT 
GGTAGCCATA AGAATCGACC ATTCAAGGAC GAGAGCCACA AGGGCAGCGC CGAGACGATG 
GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG GAGGATTTAG GTCTCGACGA GGAACTGGAC 
GAGGAGGGCG AATGCGAGGA GGGCCCGCTC GACGGTGATA TCATTATTCA TGCACACGAC 
GAGGATATAC TCGATGAATA TCCAGCTGAT TGCTGCCCCG ATTCGTACTA TAAGAAATTT 
CCGATCTTAG CCGGTGACGA TGACTCGCCG TTCTGGCAAG GATGGGGCAA TTTACGACTG 
AAAACTTTTC AATTAATTGA AAATAAATAT TTTGAAACAG CTGTTATCAC TATGATTTTA 
ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT GTACATCTGC CACAAAGACC CATACTGCAG 
GATATTTTAT ACTATATGGA CAGAATATTT ACGGTTATAT TCTTCTTGGA AATGTTAATC 
AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC TTCACCAACG CGTGGTGTTG GCTCGATTTC 
GTGATTGTCA TGGTATCGCT TATCAACTTC GTTGCTTCAC TTGTTGGAGC TGGTGGTATT 
CAAGCCTTCA AGACTATGCG AACGTTAAGA GCACTGAGAC CACTACGTGC CATGTCCCGT 
ATGCAGGGCA TGAGGGTCGT CGTTAATGCG CTGGTACAAG CTATACCGTC CATCTTCAAT 
GTGCTATTGG TGTGTCTAAT ATTTTGGCTA ATTTTTGCCA TAATGGGTGT ACAGCTTTTT 
GCTGGAAAAT ATTTTAAGTG CGAGGACATG AATGGCACGA AGCTCAGCCA CGAGATCATA 
CCAAATCGCA ATGCCTGCGA GAGCGAGAAC TACACGTGGG TGAATTCAGC AATGAATTTC 
GATCATGTAG GTAACGCGTA TCTGTGCCTT TTCCAAGTGG CCACCTTCAA AGGCTGGATA 
CAAATCATGA ACGATGCTAT CGATTCACGA GAGGTGGACA AGCAACCAAT TCGTGAAACG 
AACATCTACA TGTATTTATA TTTCGTATTC TTCATCATAT TTGGATCATT TTTCACACTC 
AATCTGTTCA TTGGTGTTAT CATTGATAAT TTTAATGAGC AAAAGAAAAA AGCAGGTGGA 
TCATTAGAAA TGTTCATGAC AGAAGATCAG AAAAAGTACT ATAATGCTAT GAAAAAGATG 
GGCTCTAAAA AACCATTAAA AGCCATTCCA AGACCAAGGT GGCGACCACA AGCAATAGTC 
TTTGAAATAG TAACCGATAA GAAATTCGAT ATAATCATTA TGTTATTCAT TGGTCTGAAC 
ATGTTCACCA TGACCCTCGA TCGTTACGAT GCGTCGGACA CGTATAACGC GGTCCTAGAC 
TATCTCAATG CGATATTCGT AGTTATTTTC AGTTCCGAAT GTCTATTAAA AATATTCGCT 
TTACGATATC ACTATTTTAT TGAGCCATGG AATTTATTTG ATGTAGTAGT TGTCATTTTA 
TCCATCTTAG GTCTTGTACT TAGCGATATT ATCGAGAAGT ACTTCGTGTC GCCGACCCTG 
CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC CGTGTCCTTC GACTGGTGAA GGGAGCCAAG 
GGCATTCGGA CACTGCTCTT CGCGTTGGCC ATGTCGCTGC CGGCCCTGTT CAACATCTGC 
CTGCTGCTGT TCCTGGTCAT GTTCATCTTT GCCATTTTCG GCATGTCGTT CTTCATGCAC 
GTGAAGGAGA AGAGCGGCAT CAACGACGTC TACAACTTCA AGACCTTTGG CCAGAGCATG 
ATCCTGCTCT TTCAGATGTC GACGTCAGCC GGTTGGGATG GTGTACTGGA CGCCATTATC 
AATGAGGAAG CATGCGATCC ACCCGACAAC GACAAAGGCT ATCCGGGCAA TTGTGGTTCA 
GCGACCGTTG GAATAACGTT TCTCCTCTCA TACCTAGTTA TAAGCTTTTT GATAGTTATT 
AATATGTACA TTGCTGTCAT TCTCGAGAAC TATAGTCAGG CCACCGAGGA CGTGCAAGAG 
GGTCTAACCG ACGACGACTA CGACATGTAC TATGAGATCT GGCAGCAATT CGATCCGGAG 
GGCACCCAGT ACATACGCTA TGATCAGCTG TCCGAATTCC TGGACGTACT GGAGCCCCCG 
CTGCAGATCC ACAAACCGAA CAAGTACAAG ATCATATCGA TGGACATACC CATCTGTCGC 
GGTGACCTCA TGTACTGCGT CGACATCCTC GACGCCCTTA CGAAAGACTT CTTTGCGCGG 
AAGGGCAATC CGATAGAGGA GACGGGTGAG ATTGGTGAGA TAGCGGCCCG CCCGGATACG 
GAGGGCTACG AGCCCGTCTC ATCAACGCTG TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG 
CTAATCCAGC ACGCCTGGCG AAAGCACAAG GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG 
CCGGATACGG ATCATGGCGA TGGCGGTGAT CCGGATGCCG GGGACCCGGC GCCCGATGAA 
GCAACGGACG GCGATGCGCC CGCTGGTGGA GATGGTAGTG TTAACGGTAC TGCAGAAGGA 
GCTGCCGATG CCGATGAGAG TAATGTAAAT AGTCCGGGTG AGGATGCAGC GGCGGCGGCA 
GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC ACGACGACGG CGGGAAGTCC CGGAGCGGGT 
AGCGCCGGGC GACAGACCGC CGTTCTCGTG GAGAGCGACG GGTTCGTGAC GAAGAACGGC 
CACAAGGTGG TCATCCACTC GCGATCGCCG AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188613.1
CDS	269..6508
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201684.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant R, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201684.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGAGGACG ATGACGAGGA TGGGCCGACG TTCAAAGACA AGGCACTCGA AGTGATCCTC 
AAAGGCATCG ATGTGTTTTG TGTGTGGGAC TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG 
TGGGTATCGC TCATCGTCTT CGATCCCTTC GTCGAGCTCT TCATCACGCT GTGCATTGTG 
GTCAACACGA TGTTCATGGC AATGGATCAC CACGATATGA ACAAGGAGAT GGAACGCGTG 
CTCAAGAGTG GCAACTATTT CTTCACCGCC ACCTTTGCCA TCGAGGCCAC CATGAAGCTA 
ATGGCCATGA GCCCCAAGTA CTATTTCCAG GAGGGCTGGA ACATCTTCGA CTTCATTATC 
GTGGCCCTAT CGCTATTGGA ACTGGGACTC GAGGGTGTCC AGGGTCTGTC CGTATTGCGT 
TCCTTTCGAT TGCTGCGTGT TTTCAAGCTG GCCAAATCGT GGCCGACGCT GAATTTGCTC 
ATATCGATTA TGGGCCGCAC TGTCGGCGCC CTCGGCAATC TGACCTTCGT CCTGTGCATT 
ATTATCTTCA TATTCGCGGT CATGGGCATG CAGCTGTTTG GCAAGAACTA CACAGATCAC 
AAGGACCGCT TTCCGGATGG CGACCTGCCG CGCTGGAACT TCACCGACTT TATGCACAGC 
TTCATGATCG TGTTCCGGGT GCTCTGCGGA GAATGGATCG AGTCCATGTG GGACTGCATG 
TACGTGGGCG ATGTCTCGTG CATTCCCTTC TTCTTGGCCA CCGTTGTCAT CGGCAATCTT 
GTGGTACTTA ACCTTTTCTT AGCCTTGCTT TTGTCCAATT TTGGCTCATC TAGCTTATCA 
GCGCCGACTG CCGATAACGA TACGAATAAA ATAGCCGAGG CCTTCAATCG AATTGGCCGA 
TTTAAAAGTT GGGTTAAGCG TAATATTGCT GATTGTTTCA AGTTAATACG TAACAAATTG 
ACAAATCAAA TAAGTGATCA ACCATCAGAG CATGGTGACA ACGAACTGGA GCTGGGCCAC 
GACGAGATCC TCGCCGACGG CCTCATCAAG AAGGGGATCA AGGAGCAGAC GCAACTGGAG 
GTGGCCATCG GGGATGGCAT GGAATTCACG ATACACGGCG ACATGAAGAA CAACAAGCCG 
AAGAAATCCA AATATCTAAA TAACGCAACG GACGACGACA CTGCCAGCAT TAACTCATAT 
GGTAGCCATA AGAATCGACC ATTCAAGGAC GAGAGCCACA AGGGCAGCGC CGAGACGATG 
GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG GAGGATTTAG GTCTCGACGA GGAACTGGAC 
GAGGAGGGCG AATGCGAGGA GGGCCCGCTC GACGGTGATA TCATTATTCA TGCACACGAC 
GAGGATATAC TCGATGAATA TCCAGCTGAT TGCTGCCCCG ATTCGTACTA TAAGAAATTT 
CCGATCTTAG CCGGTGACGA TGACTCGCCG TTCTGGCAAG GATGGGGCAA TTTACGACTG 
AAAACTTTTC AATTAATTGA AAATAAATAT TTTGAAACAG CTGTTATCAC TATGATTTTA 
ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT GTACATCTGC CACAAAGACC CATACTGCAG 
GATATTTTAT ACTATATGGA CAGAATATTT ACGGTTATAT TCTTCTTGGA AATGTTAATC 
AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC TTCACCAACG CGTGGTGTTG GCTCGATTTC 
GTGATTGTCA TGGTATCGCT TATCAACTTC GTTGCTTCAC TTGTTGGAGC TGGTGGTATT 
CAAGCCTTCA AGACTATGCG AACGTTAAGA GCACTGAGAC CACTACGTGC CATGTCCCGT 
ATGCAGGGCA TGAGGGTCGT CGTTAATGCG CTGGTACAAG CTATACCGTC CATCTTCAAT 
GTGCTATTGG TGTGTCTAAT ATTTTGGCTA ATTTTTGCCA TAATGGGTGT ACAGCTTTTT 
GCTGGAAAAT ATTTTAAGTG CGAGGACATG AATGGCACGA AGCTCAGCCA CGAGATCATA 
CCAAATCGCA ATGCCTGCGA GAGCGAGAAC TACACGTGGG TGAATTCAGC AATGAATTTC 
GATCATGTAG GTAACGCGTA TCTGTGCCTT TTCCAAGTGG CCACCTTCAA AGGCTGGATA 
CAAATCATGA ACGATGCTAT CGATTCACGA GAGGTGGACA AGCAACCAAT TCGTGAAACG 
AACATCTACA TGTATTTATA TTTCGTATTC TTCATCATAT TTGGATCATT TTTCACACTC 
AATCTGTTCA TTGGTGTTAT CATTGATAAT TTTAATGAGC AAAAGAAAAA AGCAGGTGGA 
TCATTAGAAA TGTTCATGAC AGAAGATCAG AAAAAGTACT ATAATGCTAT GAAAAAGATG 
GGCTCTAAAA AACCATTAAA AGCCATTCCA AGACCAAGGT GGCGACCACA AGCAATAGTC 
TTTGAAATAG TAACCGATAA GAAATTCGAT ATAATCATTA TGTTATTCAT TGGTCTGAAC 
ATGTTCACCA TGACCCTCGA TCGTTACGAT GCGTCGGACA CGTATAACGC GGTCCTAGAC 
TATCTCAATG CGATATTCGT AGTTATTTTC AGTTCCGAAT GTCTATTAAA AATATTCGCT 
TTACGATATC ACTATTTTAT TGAGCCATGG AATTTATTTG ATGTAGTAGT TGTCATTTTA 
TCCATCTTAG GTCTTGTACT TAGCGATATT ATCGAGAAGT ACTTCGTGTC GCCGACCCTG 
CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC CGTGTCCTTC GACTGGTGAA GGGAGCCAAG 
GGCATTCGGA CACTGCTCTT CGCGTTGGCC ATGTCGCTGC CGGCCCTGTT CAACATCTGC 
CTGCTGCTGT TCCTGGTCAT GTTCATCTTT GCCATTTTCG GCATGTCGTT CTTCATGCAC 
GTGAAGGAGA AGAGCGGCAT CAACGACGTC TACAACTTCA AGACCTTTGG CCAGAGCATG 
ATCCTGCTCT TTCAGATGTC GACGTCAGCC GGTTGGGATG GTGTACTGGA CGCCATTATC 
AATGAGGAAG CATGCGATCC ACCCGACAAC GACAAAGGCT ATCCGGGCAA TTGTGGTTCA 
GCGACCGTTG GAATAACGTT TCTCCTCTCA TACCTAGTTA TAAGCTTTTT GATAGTTATT 
AATATGTACA TTGCTGTCAT TCTCGAGAAC TATAGTCAGG CCACCGAGGA CGTGCAAGAG 
GGTCTAACCG ACGACGACTA CGACATGTAC TATGAGATCT GGCAGCAATT CGATCCGGAG 
GGCACCCAGT ACATACGCTA TGATCAGCTG TCCGAATTCC TGGACGTACT GGAGCCCCCG 
CTGCAGATCC ACAAACCGAA CAAGTACAAG ATCATATCGA TGGACATACC CATCTGTCGC 
GGTGACCTCA TGTACTGCGT CGACATCCTC GACGCCCTTA CGAAAGACTT CTTTGCGCGG 
AAGGGCAATC CGATAGAGGA GACGGGTGAG ATTGGTGAGA TAGCGGCCCG CCCGGATACG 
GAGGGCTACG AGCCCGTCTC ATCAACGCTG TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG 
CTAATCCAGC ACGCCTGGCG AAAGCACAAG GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG 
CCGGATACGG ATCATGGCGA TGGCGGTGAT CCGGATGCCG GGGACCCGGC GCCCGATGAA 
GCAACGGACG GCGATGCGCC CGCTGGTGGA GATGGTAGTG TTAACGGTAC TGCAGAAGGA 
GCTGCCGATG CCGATGAGAG TAATGTAAAT AGTCCGGGTG AGGATGCAGC GGCGGCGGCA 
GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC ACGACGACGG CGGGAAGTCC CGGAGCGGGT 
AGCGCCGGGC GACAGACCGC CGTTCTCGTG GAGAGCGACG GGTTCGTGAC GAAGAACGGC 
CACAAGGTGG TCATCCACTC GCGATCGCCG AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201673.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188602.1 Drosophila melanogaster paralytic (para), transcript variant G, mRNA.

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CloneID	OFa01808
Clone ID Related Accession (Same CDS sequence)	NM_001201673.1
Accession Version	NM_001201673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTATTCAA ACTGGCCAAG 
TCTTGGCCCA CACTTAATTT ACTCATTTCG ATTATGGGAC GCACCATGGG CGCTTTGGGT 
AATCTGACAT TTGTACTTTG CATTATCATC TTCATCTTTG CGGTGATGGG AATGCAACTG 
TTCGGAAAGA ATTATCATGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGGACGAC 
GACACTGCCA GCATTAACTC ATATGGTAGC CATAAGAATC GACCATTCAA GGACGAGAGC 
CACAAGGGCA GCGCCGAGAC GATGGAGGGC GAGGAGAAGC GCGACGCCAG CAAGGAGGAT 
TTAGGTCTCG ACGAGGAACT GGACGAGGAG GGCGAATGCG AGGAGGGCCC GCTCGACGGT 
GATATCATTA TTCATGCACA CGACGAGGAT ATACTCGATG AATATCCAGC TGATTGCTGC 
CCCGATTCGT ACTATAAGAA ATTTCCGATC TTAGCCGGTG ACGATGACTC GCCGTTCTGG 
CAAGGATGGG GCAATTTACG ACTGAAAACT TTTCAATTAA TTGAAAATAA ATATTTTGAA 
ACAGCTGTTA TCACTATGAT TTTAATGAGT AGCTTAGCTT TGGCATTAGA AGATGTACAT 
CTGCCACAAA GACCCATACT GCAGGATATT TTATACTATA TGGACAGAAT ATTTACGGTT 
ATATTCTTCT TGGAAATGTT AATCAAGTGG TTGGCGCTCG GCTTCAAAGT GTACTTCACC 
AACGCGTGGT GTTGGCTCGA TTTCGTGATT GTCATGGTAT CGCTTATCAA CTTCGTTGCT 
TCACTTGTTG GAGCTGGTGG TATTCAAGCC TTCAAGACTA TGCGAACGTT AAGAGCACTG 
AGACCACTAC GTGCCATGTC CCGTATGCAG GGCATGAGGG TCGTCGTTAA TGCGCTGGTA 
CAAGCTATAC CGTCCATCTT CAATGTGCTA TTGGTGTGTC TAATATTTTG GCTAATTTTT 
GCCATAATGG GTGTACAGCT TTTTGCTGGA AAATATTTTA AGTGCGAGGA CATGAATGGC 
ACGAAGCTCA GCCACGAGAT CATACCAAAT CGCAATGCCT GCGAGAGCGA GAACTACACG 
TGGGTGAATT CAGCAATGAA TTTCGATCAT GTAGGTAACG CGTATCTGTG CCTTTTCCAA 
GTGGCCACCT TCAAAGGCTG GATACAAATC ATGAACGATG CTATCGATTC ACGAGAGGTG 
GACAAGCAAC CAATTCGTGA AACGAACATC TACATGTATT TATATTTCGT ATTCTTCATC 
ATATTTGGAT CATTTTTCAC ACTCAATCTG TTCATTGGTG TTATCATTGA TAATTTTAAT 
GAGCAAAAGA AAAAAGCAGG TGGATCATTA GAAATGTTCA TGACAGAAGA TCAGAAAAAG 
TACTATAATG CTATGAAAAA GATGGGCTCT AAAAAACCAT TAAAAGCCAT TCCAAGACCA 
AGGTGGCGAC CACAAGCAAT AGTCTTTGAA ATAGTAACCG ATAAGAAATT CGATATAATC 
ATTATGTTAT TCATTGGTCT GAACATGTTC ACCATGACCC TCGATCGTTA CGATGCGTCG 
GACACGTATA ACGCGGTCCT AGACTATCTC AATGCGATAT TCGTAGTTAT TTTCAGTTCC 
GAATGTCTAT TAAAAATATT CGCTTTACGA TATCACTATT TTATTGAGCC ATGGAATTTA 
TTTGATGTAG TAGTTGTCAT TTTATCCATC TTAGGTCTTG TACTTAGCGA TATTATCGAG 
AAGTACTTCG TGTCGCCGAC CCTGCTCCGA GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC 
CTTCGACTGG TGAAGGGAGC CAAGGGCATT CGGACACTGC TCTTCGCGTT GGCCATGTCG 
CTGCCGGCCC TGTTCAACAT CTGCCTGCTG CTGTTCCTGG TCATGTTCAT CTTTGCCATT 
TTCGGCATGT CGTTCTTCAT GCACGTGAAG GAGAAGAGCG GCATCAACGA CGTCTACAAC 
TTCAAGACCT TTGGCCAGAG CATGATCCTG CTCTTTCAGA TGTCGACGTC AGCCGGTTGG 
GATGGTGTAC TGGACGCCAT TATCAATGAG GAAGCATGCG ATCCACCCGA CAACGACAAA 
GGCTATCCGG GCAATTGTGG TTCAGCGACC GTTGGAATAA CGTTTCTCCT CTCATACCTA 
GTTATAAGCT TTTTGATAGT TATTAATATG TACATTGCTG TCATTCTCGA GAACTATAGT 
CAGGCCACCG AGGACGTGCA AGAGGGTCTA ACCGACGACG ACTACGACAT GTACTATGAG 
ATCTGGCAGC AATTCGATCC GGAGGGCACC CAGTACATAC GCTATGATCA GCTGTCCGAA 
TTCCTGGACG TACTGGAGCC CCCGCTGCAG ATCCACAAAC CGAACAAGTA CAAGATCATA 
TCGATGGACA TACCCATCTG TCGCGGTGAC CTCATGTACT GCGTCGACAT CCTCGACGCC 
CTTACGAAAG ACTTCTTTGC GCGGAAGGGC AATCCGATAG AGGAGACGGG TGAGATTGGT 
GAGATAGCGG CCCGCCCGGA TACGGAGGGC TACGAGCCCG TCTCATCAAC GCTGTGGCGT 
CAGCGTGAGG AGTACTGCGC CCGGCTAATC CAGCACGCCT GGCGAAAGCA CAAGGCGCGC 
GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT ACGGATCATG GCGATGGCGG TGATCCGGAT 
GCCGGGGACC CGGCGCCCGA TGAAGCAACG GACGGCGATG CGCCCGCTGG TGGAGATGGT 
AGTGTTAACG GTACTGCAGA AGGAGCTGCC GATGCCGATG AGAGTAATGT AAATAGTCCG 
GGTGAGGATG CAGCGGCGGC GGCAGCAGCA GCAGCAGCAG CGGCGGCGGC GGGCACGACG 
ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC GGGCGACAGA CCGCCGTTCT CGTGGAGAGC 
GACGGGTTCG TGACGAAGAA CGGCCACAAG GTGGTCATCC ACTCGCGATC GCCGAGCATC 
ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188602.1
CDS	269..6532
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201673.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201673.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTATTCAA ACTGGCCAAG 
TCTTGGCCCA CACTTAATTT ACTCATTTCG ATTATGGGAC GCACCATGGG CGCTTTGGGT 
AATCTGACAT TTGTACTTTG CATTATCATC TTCATCTTTG CGGTGATGGG AATGCAACTG 
TTCGGAAAGA ATTATCATGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGGACGAC 
GACACTGCCA GCATTAACTC ATATGGTAGC CATAAGAATC GACCATTCAA GGACGAGAGC 
CACAAGGGCA GCGCCGAGAC GATGGAGGGC GAGGAGAAGC GCGACGCCAG CAAGGAGGAT 
TTAGGTCTCG ACGAGGAACT GGACGAGGAG GGCGAATGCG AGGAGGGCCC GCTCGACGGT 
GATATCATTA TTCATGCACA CGACGAGGAT ATACTCGATG AATATCCAGC TGATTGCTGC 
CCCGATTCGT ACTATAAGAA ATTTCCGATC TTAGCCGGTG ACGATGACTC GCCGTTCTGG 
CAAGGATGGG GCAATTTACG ACTGAAAACT TTTCAATTAA TTGAAAATAA ATATTTTGAA 
ACAGCTGTTA TCACTATGAT TTTAATGAGT AGCTTAGCTT TGGCATTAGA AGATGTACAT 
CTGCCACAAA GACCCATACT GCAGGATATT TTATACTATA TGGACAGAAT ATTTACGGTT 
ATATTCTTCT TGGAAATGTT AATCAAGTGG TTGGCGCTCG GCTTCAAAGT GTACTTCACC 
AACGCGTGGT GTTGGCTCGA TTTCGTGATT GTCATGGTAT CGCTTATCAA CTTCGTTGCT 
TCACTTGTTG GAGCTGGTGG TATTCAAGCC TTCAAGACTA TGCGAACGTT AAGAGCACTG 
AGACCACTAC GTGCCATGTC CCGTATGCAG GGCATGAGGG TCGTCGTTAA TGCGCTGGTA 
CAAGCTATAC CGTCCATCTT CAATGTGCTA TTGGTGTGTC TAATATTTTG GCTAATTTTT 
GCCATAATGG GTGTACAGCT TTTTGCTGGA AAATATTTTA AGTGCGAGGA CATGAATGGC 
ACGAAGCTCA GCCACGAGAT CATACCAAAT CGCAATGCCT GCGAGAGCGA GAACTACACG 
TGGGTGAATT CAGCAATGAA TTTCGATCAT GTAGGTAACG CGTATCTGTG CCTTTTCCAA 
GTGGCCACCT TCAAAGGCTG GATACAAATC ATGAACGATG CTATCGATTC ACGAGAGGTG 
GACAAGCAAC CAATTCGTGA AACGAACATC TACATGTATT TATATTTCGT ATTCTTCATC 
ATATTTGGAT CATTTTTCAC ACTCAATCTG TTCATTGGTG TTATCATTGA TAATTTTAAT 
GAGCAAAAGA AAAAAGCAGG TGGATCATTA GAAATGTTCA TGACAGAAGA TCAGAAAAAG 
TACTATAATG CTATGAAAAA GATGGGCTCT AAAAAACCAT TAAAAGCCAT TCCAAGACCA 
AGGTGGCGAC CACAAGCAAT AGTCTTTGAA ATAGTAACCG ATAAGAAATT CGATATAATC 
ATTATGTTAT TCATTGGTCT GAACATGTTC ACCATGACCC TCGATCGTTA CGATGCGTCG 
GACACGTATA ACGCGGTCCT AGACTATCTC AATGCGATAT TCGTAGTTAT TTTCAGTTCC 
GAATGTCTAT TAAAAATATT CGCTTTACGA TATCACTATT TTATTGAGCC ATGGAATTTA 
TTTGATGTAG TAGTTGTCAT TTTATCCATC TTAGGTCTTG TACTTAGCGA TATTATCGAG 
AAGTACTTCG TGTCGCCGAC CCTGCTCCGA GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC 
CTTCGACTGG TGAAGGGAGC CAAGGGCATT CGGACACTGC TCTTCGCGTT GGCCATGTCG 
CTGCCGGCCC TGTTCAACAT CTGCCTGCTG CTGTTCCTGG TCATGTTCAT CTTTGCCATT 
TTCGGCATGT CGTTCTTCAT GCACGTGAAG GAGAAGAGCG GCATCAACGA CGTCTACAAC 
TTCAAGACCT TTGGCCAGAG CATGATCCTG CTCTTTCAGA TGTCGACGTC AGCCGGTTGG 
GATGGTGTAC TGGACGCCAT TATCAATGAG GAAGCATGCG ATCCACCCGA CAACGACAAA 
GGCTATCCGG GCAATTGTGG TTCAGCGACC GTTGGAATAA CGTTTCTCCT CTCATACCTA 
GTTATAAGCT TTTTGATAGT TATTAATATG TACATTGCTG TCATTCTCGA GAACTATAGT 
CAGGCCACCG AGGACGTGCA AGAGGGTCTA ACCGACGACG ACTACGACAT GTACTATGAG 
ATCTGGCAGC AATTCGATCC GGAGGGCACC CAGTACATAC GCTATGATCA GCTGTCCGAA 
TTCCTGGACG TACTGGAGCC CCCGCTGCAG ATCCACAAAC CGAACAAGTA CAAGATCATA 
TCGATGGACA TACCCATCTG TCGCGGTGAC CTCATGTACT GCGTCGACAT CCTCGACGCC 
CTTACGAAAG ACTTCTTTGC GCGGAAGGGC AATCCGATAG AGGAGACGGG TGAGATTGGT 
GAGATAGCGG CCCGCCCGGA TACGGAGGGC TACGAGCCCG TCTCATCAAC GCTGTGGCGT 
CAGCGTGAGG AGTACTGCGC CCGGCTAATC CAGCACGCCT GGCGAAAGCA CAAGGCGCGC 
GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT ACGGATCATG GCGATGGCGG TGATCCGGAT 
GCCGGGGACC CGGCGCCCGA TGAAGCAACG GACGGCGATG CGCCCGCTGG TGGAGATGGT 
AGTGTTAACG GTACTGCAGA AGGAGCTGCC GATGCCGATG AGAGTAATGT AAATAGTCCG 
GGTGAGGATG CAGCGGCGGC GGCAGCAGCA GCAGCAGCAG CGGCGGCGGC GGGCACGACG 
ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC GGGCGACAGA CCGCCGTTCT CGTGGAGAGC 
GACGGGTTCG TGACGAAGAA CGGCCACAAG GTGGTCATCC ACTCGCGATC GCCGAGCATC 
ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201688.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188617.1 Drosophila melanogaster paralytic (para), transcript variant V, mRNA.

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CloneID	OFa01809
Clone ID Related Accession (Same CDS sequence)	NM_001201688.1
Accession Version	NM_001201688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform V
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTATTCAA ACTGGCCAAG 
TCTTGGCCCA CACTTAATTT ACTCATTTCG ATTATGGGAC GCACCATGGG CGCTTTGGGT 
AATCTGACAT TTGTACTTTG CATTATCATC TTCATCTTTG CGGTGATGGG AATGCAACTG 
TTCGGAAAGA ATTATCATGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGGCAAAGGC 
GTTTGTCGTT GTATATCTGC AGAGCATGGT GACAACGAAC TGGAGCTGGG CCACGACGAG 
ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG ATCAAGGAGC AGACGCAACT GGAGGTGGCC 
ATCGGGGATG GCATGGAATT CACGATACAC GGCGACATGA AGAACAACAA GCCGAAGAAA 
TCCAAATATC TAAATAACGC AACGGACGAC GACACTGCCA GCATTAACTC ATATGGTAGC 
CATAAGAATC GACCATTCAA GGACGAGAGC CACAAGGGCA GCGCCGAGAC GATGGAGGGC 
GAGGAGAAGC GCGACGCCAG CAAGGAGGAT TTAGGTCTCG ACGAGGAACT GGACGAGGAG 
GGCGAATGCG AGGAGGGCCC GCTCGACGGT GATATCATTA TTCATGCACA CGACGAGGAT 
ATACTCGATG AATATCCAGC TGATTGCTGC CCCGATTCGT ACTATAAGAA ATTTCCGATC 
TTAGCCGGTG ACGATGACTC GCCGTTCTGG CAAGGATGGG GCAATTTACG ACTGAAAACT 
TTTCAATTAA TTGAAAATAA ATATTTTGAA ACAGCTGTTA TCACTATGAT TTTAATGAGT 
AGCTTAGCTT TGGCATTAGA AGATGTACAT CTGCCACAAA GACCCATACT GCAGGATATT 
TTATACTATA TGGACAGAAT ATTTACGGTT ATATTCTTCT TGGAAATGTT AATCAAGTGG 
TTGGCGCTCG GCTTCAAAGT GTACTTCACC AACGCGTGGT GTTGGCTCGA TTTCGTGATT 
GTCATGGTAT CGCTTATCAA CTTCGTTGCT TCACTTGTTG GAGCTGGTGG TATTCAAGCC 
TTCAAGACTA TGCGAACGTT AAGAGCACTG AGACCACTAC GTGCCATGTC CCGTATGCAG 
GGCATGAGGG TCGTCGTTAA TGCGCTGGTA CAAGCTATAC CGTCCATCTT CAATGTGCTA 
TTGGTGTGTC TAATATTTTG GCTAATTTTT GCCATAATGG GTGTACAGCT TTTTGCTGGA 
AAATATTTTA AGTGCGAGGA CATGAATGGC ACGAAGCTCA GCCACGAGAT CATACCAAAT 
CGCAATGCCT GCGAGAGCGA GAACTACACG TGGGTGAATT CAGCAATGAA TTTCGATCAT 
GTAGGTAACG CGTATCTGTG CCTTTTCCAA GTGGCCACCT TCAAAGGCTG GATACAAATC 
ATGAACGATG CTATCGATTC ACGAGAGGTG GACAAGCAAC CAATTCGTGA AACGAACATC 
TACATGTATT TATATTTCGT ATTCTTCATC ATATTTGGAT CATTTTTCAC ACTCAATCTG 
TTCATTGGTG TTATCATTGA TAATTTTAAT GAGCAAAAGA AAAAAGCAGG TGGATCATTA 
GAAATGTTCA TGACAGAAGA TCAGAAAAAG TACTATAATG CTATGAAAAA GATGGGCTCT 
AAAAAACCAT TAAAAGCCAT TCCAAGACCA AGGTGGCGAC CACAAGCAAT AGTCTTTGAA 
ATAGTAACCG ATAAGAAATT CGATATAATC ATTATGTTAT TCATTGGTCT GAACATGTTC 
ACCATGACCC TCGATCGTTA CGATGCGTCG GACACGTATA ACGCGGTCCT AGACTATCTC 
AATGCGATAT TCGTAGTTAT TTTCAGTTCC GAATGTCTAT TAAAAATATT CGCTTTACGA 
TATCACTATT TTATTGAGCC ATGGAATTTA TTTGATGTAG TAGTTGTCAT TTTATCCATC 
TTAGGTCTTG TACTTAGCGA TATTATCGAG AAGTACTTCG TGTCGCCGAC CCTGCTCCGA 
GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC CTTCGACTGG TGAAGGGAGC CAAGGGCATT 
CGGACACTGC TCTTCGCGTT GGCCATGTCG CTGCCGGCCC TGTTCAACAT CTGCCTGCTG 
CTGTTCCTGG TCATGTTCAT CTTTGCCATT TTCGGCATGT CGTTCTTCAT GCACGTGAAG 
GAGAAGAGCG GCATCAACGA CGTCTACAAC TTCAAGACCT TTGGCCAGAG CATGATCCTG 
CTCTTTCAGA TGTCGACGTC AGCCGGTTGG GATGGTGTAC TGGACGCCAT TATCAATGAG 
GAAGCATGCG ATCCACCCGA CAACGACAAA GGCTATCCGG GCAATTGTGG TTCAGCGACC 
GTTGGAATAA CGTTTCTCCT CTCATACCTA GTTATAAGCT TTTTGATAGT TATTAATATG 
TACATTGCTG TCATTCTCGA GAACTATAGT CAGGCCACCG AGGACGTGCA AGAGGGTCTA 
ACCGACGACG ACTACGACAT GTACTATGAG ATCTGGCAGC AATTCGATCC GGAGGGCACC 
CAGTACATAC GCTATGATCA GCTGTCCGAA TTCCTGGACG TACTGGAGCC CCCGCTGCAG 
ATCCACAAAC CGAACAAGTA CAAGATCATA TCGATGGACA TACCCATCTG TCGCGGTGAC 
CTCATGTACT GCGTCGACAT CCTCGACGCC CTTACGAAAG ACTTCTTTGC GCGGAAGGGC 
AATCCGATAG AGGAGACGGG TGAGATTGGT GAGATAGCGG CCCGCCCGGA TACGGAGGGC 
TACGAGCCCG TCTCATCAAC GCTGTGGCGT CAGCGTGAGG AGTACTGCGC CCGGCTAATC 
CAGCACGCCT GGCGAAAGCA CAAGGCGCGC GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT 
ACGGATCATG GCGATGGCGG TGATCCGGAT GCCGGGGACC CGGCGCCCGA TGAAGCAACG 
GACGGCGATG CGCCCGCTGG TGGAGATGGT AGTGTTAACG GTACTGCAGA AGGAGCTGCC 
GATGCCGATG AGAGTAATGT AAATAGTCCG GGTGAGGATG CAGCGGCGGC GGCAGCAGCA 
GCAGCAGCAG CGGCGGCGGC GGGCACGACG ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC 
GGGCGACAGA CCGCCGTTCT CGTGGAGAGC GACGGGTTCG TGACGAAGAA CGGCCACAAG 
GTGGTCATCC ACTCGCGATC GCCGAGCATC ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188617.1
CDS	269..6562
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201688.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant V, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201688.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTATTCAA ACTGGCCAAG 
TCTTGGCCCA CACTTAATTT ACTCATTTCG ATTATGGGAC GCACCATGGG CGCTTTGGGT 
AATCTGACAT TTGTACTTTG CATTATCATC TTCATCTTTG CGGTGATGGG AATGCAACTG 
TTCGGAAAGA ATTATCATGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGGCAAAGGC 
GTTTGTCGTT GTATATCTGC AGAGCATGGT GACAACGAAC TGGAGCTGGG CCACGACGAG 
ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG ATCAAGGAGC AGACGCAACT GGAGGTGGCC 
ATCGGGGATG GCATGGAATT CACGATACAC GGCGACATGA AGAACAACAA GCCGAAGAAA 
TCCAAATATC TAAATAACGC AACGGACGAC GACACTGCCA GCATTAACTC ATATGGTAGC 
CATAAGAATC GACCATTCAA GGACGAGAGC CACAAGGGCA GCGCCGAGAC GATGGAGGGC 
GAGGAGAAGC GCGACGCCAG CAAGGAGGAT TTAGGTCTCG ACGAGGAACT GGACGAGGAG 
GGCGAATGCG AGGAGGGCCC GCTCGACGGT GATATCATTA TTCATGCACA CGACGAGGAT 
ATACTCGATG AATATCCAGC TGATTGCTGC CCCGATTCGT ACTATAAGAA ATTTCCGATC 
TTAGCCGGTG ACGATGACTC GCCGTTCTGG CAAGGATGGG GCAATTTACG ACTGAAAACT 
TTTCAATTAA TTGAAAATAA ATATTTTGAA ACAGCTGTTA TCACTATGAT TTTAATGAGT 
AGCTTAGCTT TGGCATTAGA AGATGTACAT CTGCCACAAA GACCCATACT GCAGGATATT 
TTATACTATA TGGACAGAAT ATTTACGGTT ATATTCTTCT TGGAAATGTT AATCAAGTGG 
TTGGCGCTCG GCTTCAAAGT GTACTTCACC AACGCGTGGT GTTGGCTCGA TTTCGTGATT 
GTCATGGTAT CGCTTATCAA CTTCGTTGCT TCACTTGTTG GAGCTGGTGG TATTCAAGCC 
TTCAAGACTA TGCGAACGTT AAGAGCACTG AGACCACTAC GTGCCATGTC CCGTATGCAG 
GGCATGAGGG TCGTCGTTAA TGCGCTGGTA CAAGCTATAC CGTCCATCTT CAATGTGCTA 
TTGGTGTGTC TAATATTTTG GCTAATTTTT GCCATAATGG GTGTACAGCT TTTTGCTGGA 
AAATATTTTA AGTGCGAGGA CATGAATGGC ACGAAGCTCA GCCACGAGAT CATACCAAAT 
CGCAATGCCT GCGAGAGCGA GAACTACACG TGGGTGAATT CAGCAATGAA TTTCGATCAT 
GTAGGTAACG CGTATCTGTG CCTTTTCCAA GTGGCCACCT TCAAAGGCTG GATACAAATC 
ATGAACGATG CTATCGATTC ACGAGAGGTG GACAAGCAAC CAATTCGTGA AACGAACATC 
TACATGTATT TATATTTCGT ATTCTTCATC ATATTTGGAT CATTTTTCAC ACTCAATCTG 
TTCATTGGTG TTATCATTGA TAATTTTAAT GAGCAAAAGA AAAAAGCAGG TGGATCATTA 
GAAATGTTCA TGACAGAAGA TCAGAAAAAG TACTATAATG CTATGAAAAA GATGGGCTCT 
AAAAAACCAT TAAAAGCCAT TCCAAGACCA AGGTGGCGAC CACAAGCAAT AGTCTTTGAA 
ATAGTAACCG ATAAGAAATT CGATATAATC ATTATGTTAT TCATTGGTCT GAACATGTTC 
ACCATGACCC TCGATCGTTA CGATGCGTCG GACACGTATA ACGCGGTCCT AGACTATCTC 
AATGCGATAT TCGTAGTTAT TTTCAGTTCC GAATGTCTAT TAAAAATATT CGCTTTACGA 
TATCACTATT TTATTGAGCC ATGGAATTTA TTTGATGTAG TAGTTGTCAT TTTATCCATC 
TTAGGTCTTG TACTTAGCGA TATTATCGAG AAGTACTTCG TGTCGCCGAC CCTGCTCCGA 
GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC CTTCGACTGG TGAAGGGAGC CAAGGGCATT 
CGGACACTGC TCTTCGCGTT GGCCATGTCG CTGCCGGCCC TGTTCAACAT CTGCCTGCTG 
CTGTTCCTGG TCATGTTCAT CTTTGCCATT TTCGGCATGT CGTTCTTCAT GCACGTGAAG 
GAGAAGAGCG GCATCAACGA CGTCTACAAC TTCAAGACCT TTGGCCAGAG CATGATCCTG 
CTCTTTCAGA TGTCGACGTC AGCCGGTTGG GATGGTGTAC TGGACGCCAT TATCAATGAG 
GAAGCATGCG ATCCACCCGA CAACGACAAA GGCTATCCGG GCAATTGTGG TTCAGCGACC 
GTTGGAATAA CGTTTCTCCT CTCATACCTA GTTATAAGCT TTTTGATAGT TATTAATATG 
TACATTGCTG TCATTCTCGA GAACTATAGT CAGGCCACCG AGGACGTGCA AGAGGGTCTA 
ACCGACGACG ACTACGACAT GTACTATGAG ATCTGGCAGC AATTCGATCC GGAGGGCACC 
CAGTACATAC GCTATGATCA GCTGTCCGAA TTCCTGGACG TACTGGAGCC CCCGCTGCAG 
ATCCACAAAC CGAACAAGTA CAAGATCATA TCGATGGACA TACCCATCTG TCGCGGTGAC 
CTCATGTACT GCGTCGACAT CCTCGACGCC CTTACGAAAG ACTTCTTTGC GCGGAAGGGC 
AATCCGATAG AGGAGACGGG TGAGATTGGT GAGATAGCGG CCCGCCCGGA TACGGAGGGC 
TACGAGCCCG TCTCATCAAC GCTGTGGCGT CAGCGTGAGG AGTACTGCGC CCGGCTAATC 
CAGCACGCCT GGCGAAAGCA CAAGGCGCGC GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT 
ACGGATCATG GCGATGGCGG TGATCCGGAT GCCGGGGACC CGGCGCCCGA TGAAGCAACG 
GACGGCGATG CGCCCGCTGG TGGAGATGGT AGTGTTAACG GTACTGCAGA AGGAGCTGCC 
GATGCCGATG AGAGTAATGT AAATAGTCCG GGTGAGGATG CAGCGGCGGC GGCAGCAGCA 
GCAGCAGCAG CGGCGGCGGC GGGCACGACG ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC 
GGGCGACAGA CCGCCGTTCT CGTGGAGAGC GACGGGTTCG TGACGAAGAA CGGCCACAAG 
GTGGTCATCC ACTCGCGATC GCCGAGCATC ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201708.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188637.1 Drosophila melanogaster paralytic (para), transcript variant AP, mRNA.

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CloneID	OFa01810
Clone ID Related Accession (Same CDS sequence)	NM_001201708.1
Accession Version	NM_001201708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AP
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTATTCAA ACTGGCCAAG 
TCTTGGCCCA CACTTAATTT ACTCATTTCG ATTATGGGAC GCACCATGGG CGCTTTGGGT 
AATCTGACAT TTGTACTTTG CATTATCATC TTCATCTTTG CGGTGATGGG AATGCAACTG 
TTCGGAAAGA ATTATCATGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGGCAAAGGC 
GTTTGTCGTT GTATATCTGC AGAGCATGGT GACAACGAAC TGGAGCTGGG CCACGACGAG 
ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG ATCAAGGAGC AGACGCAACT GGAGGTGGCC 
ATCGGGGATG GCATGGAATT CACGATACAC GGCGACATGA AGAACAACAA GCCGAAGAAA 
TCCAAATATC TAAATAACGC AACGGACGAC GACACTGCCA GCATTAACTC ATATGGTAGC 
CATAAGAATC GACCATTCAA GGACGAGAGC CACAAGGGCA GCGCCGAGAC GATGGAGGGC 
GAGGAGAAGC GCGACGCCAG CAAGGAGGAT TTAGGTCTCG ACGAGGAACT GGACGAGGAG 
GGCGAATGCG AGGAGGGCCC GCTCGACGGT GATATCATTA TTCATGCACA CGACGAGGAT 
ATACTCGATG AATATCCAGC TGATTGCTGC CCCGATTCGT ACTATAAGAA ATTTCCGATC 
TTAGCCGGTG ACGATGACTC GCCGTTCTGG CAAGGATGGG GCAATTTACG ACTGAAAACT 
TTTCAATTAA TTGAAAATAA ATATTTTGAA ACAGCTGTTA TCACTATGAT TTTAATGAGT 
AGCTTAGCTT TGGCATTAGA AGATGTACAT CTGCCACAAA GACCCATACT GCAGGATATT 
TTATACTATA TGGACAGAAT ATTTACGGTT ATATTCTTCT TGGAAATGTT AATCAAGTGG 
TTGGCGCTCG GCTTCAAAGT GTACTTCACC AACGCGTGGT GTTGGCTCGA TTTCGTGATT 
GTCATGCTGT CGCTAATTAA TTTGGCCGCG GTCTGGTCCG GGGCCGATGA TGTGCCCGCC 
TTTCGCTCGA TGCGAACTCT GCGCGCCCTG CGACCACTGC GGGCAGTTTC GCGCTGGGAG 
GGCATGAAAG TCGTCGTTAA TGCGCTGGTA CAAGCTATAC CGTCCATCTT CAATGTGCTA 
TTGGTGTGTC TAATATTTTG GCTAATTTTT GCCATAATGG GTGTACAGCT TTTTGCTGGA 
AAATATTTTA AGTGCGAGGA CATGAATGGC ACGAAGCTCA GCCACGAGAT CATACCAAAT 
CGCAATGCCT GCGAGAGCGA GAACTACACG TGGGTGAATT CAGCAATGAA TTTCGATCAT 
GTAGGTAACG CGTATCTGTG CCTTTTCCAA GTGGCCACCT TCAAAGGCTG GATACAAATC 
ATGAACGATG CTATCGATTC ACGAGAGGTG GACAAGCAAC CAATTCGTGA AACGAACATC 
TACATGTATT TATATTTCGT ATTCTTCATC ATATTTGGAT CATTTTTCAC ACTCAATCTG 
TTCATTGGTG TTATCATTGA TAATTTTAAT GAGCAAAAGA AAAAAGCAGG TGGATCATTA 
GAAATGTTCA TGACAGAAGA TCAGAAAAAG TACTATAATG CTATGAAAAA GATGGGCTCT 
AAAAAACCAT TAAAAGCCAT TCCAAGACCA AGGTGGCGAC CACAAGCAAT AGTCTTTGAA 
ATAGTAACCG ATAAGAAATT CGATATAATC ATTATGTTAT TCATTGGTCT GAACATGTTC 
ACCATGACCC TCGATCGTTA CGATGCGTCG GACACGTATA ACGCGGTCCT AGACTATCTC 
AATGCGATAT TCGTAGTTAT TTTCAGTTCC GAATGTCTAT TAAAAATATT CGCTTTACGA 
TATCACTATT TTATTGAGCC ATGGAATTTA TTTGATGTAG TAGTTGTCAT TTTATCCATC 
TTAGGTCTTG TACTTAGCGA TATTATCGAG AAGTACTTCG TGTCGCCGAC CCTGCTCCGA 
GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC CTTCGACTGG TGAAGGGAGC CAAGGGCATT 
CGGACACTGC TCTTCGCGTT GGCCATGTCG CTGCCGGCCC TGTTCAACAT CTGCCTGCTG 
CTGTTCCTGG TCATGTTCAT CTTTGCCATT TTCGGCATGT CGTTCTTCAT GCACGTGAAG 
GAGAAGAGCG GCATCAACGA CGTCTACAAC TTCAAGACCT TTGGCCAGAG CATGATCCTG 
CTCTTTCAGA TGTCGACGTC AGCCGGTTGG GATGGTGTAC TGGACGCCAT TATCAATGAG 
GAAGCATGCG ATCCACCCGA CAACGACAAA GGCTATCCGG GCAATTGTGG TTCAGCGACC 
GTTGGAATAA CGTTTCTCCT CTCATACCTA GTTATAAGCT TTTTGATAGT TATTAATATG 
TACATTGCTG TCATTCTCGA GAACTATAGT CAGGCCACCG AGGACGTGCA AGAGGGTCTA 
ACCGACGACG ACTACGACAT GTACTATGAG ATCTGGCAGC AATTCGATCC GGAGGGCACC 
CAGTACATAC GCTATGATCA GCTGTCCGAA TTCCTGGACG TACTGGAGCC CCCGCTGCAG 
ATCCACAAAC CGAACAAGTA CAAGATCATA TCGATGGACA TACCCATCTG TCGCGGTGAC 
CTCATGTACT GCGTCGACAT CCTCGACGCC CTTACGAAAG ACTTCTTTGC GCGGAAGGGC 
AATCCGATAG AGGAGACGGG TGAGATTGGT GAGATAGCGG CCCGCCCGGA TACGGAGGGC 
TACGAGCCCG TCTCATCAAC GCTGTGGCGT CAGCGTGAGG AGTACTGCGC CCGGCTAATC 
CAGCACGCCT GGCGAAAGCA CAAGGCGCGC GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT 
ACGGATCATG GCGATGGCGG TGATCCGGAT GCCGGGGACC CGGCGCCCGA TGAAGCAACG 
GACGGCGATG CGCCCGCTGG TGGAGATGGT AGTGTTAACG GTACTGCAGA AGGAGCTGCC 
GATGCCGATG AGAGTAATGT AAATAGTCCG GGTGAGGATG CAGCGGCGGC GGCAGCAGCA 
GCAGCAGCAG CGGCGGCGGC GGGCACGACG ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC 
GGGCGACAGA CCGCCGTTCT CGTGGAGAGC GACGGGTTCG TGACGAAGAA CGGCCACAAG 
GTGGTCATCC ACTCGCGATC GCCGAGCATC ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188637.1
CDS	269..6562
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201708.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AP, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201708.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTATTCAA ACTGGCCAAG 
TCTTGGCCCA CACTTAATTT ACTCATTTCG ATTATGGGAC GCACCATGGG CGCTTTGGGT 
AATCTGACAT TTGTACTTTG CATTATCATC TTCATCTTTG CGGTGATGGG AATGCAACTG 
TTCGGAAAGA ATTATCATGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGGCAAAGGC 
GTTTGTCGTT GTATATCTGC AGAGCATGGT GACAACGAAC TGGAGCTGGG CCACGACGAG 
ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG ATCAAGGAGC AGACGCAACT GGAGGTGGCC 
ATCGGGGATG GCATGGAATT CACGATACAC GGCGACATGA AGAACAACAA GCCGAAGAAA 
TCCAAATATC TAAATAACGC AACGGACGAC GACACTGCCA GCATTAACTC ATATGGTAGC 
CATAAGAATC GACCATTCAA GGACGAGAGC CACAAGGGCA GCGCCGAGAC GATGGAGGGC 
GAGGAGAAGC GCGACGCCAG CAAGGAGGAT TTAGGTCTCG ACGAGGAACT GGACGAGGAG 
GGCGAATGCG AGGAGGGCCC GCTCGACGGT GATATCATTA TTCATGCACA CGACGAGGAT 
ATACTCGATG AATATCCAGC TGATTGCTGC CCCGATTCGT ACTATAAGAA ATTTCCGATC 
TTAGCCGGTG ACGATGACTC GCCGTTCTGG CAAGGATGGG GCAATTTACG ACTGAAAACT 
TTTCAATTAA TTGAAAATAA ATATTTTGAA ACAGCTGTTA TCACTATGAT TTTAATGAGT 
AGCTTAGCTT TGGCATTAGA AGATGTACAT CTGCCACAAA GACCCATACT GCAGGATATT 
TTATACTATA TGGACAGAAT ATTTACGGTT ATATTCTTCT TGGAAATGTT AATCAAGTGG 
TTGGCGCTCG GCTTCAAAGT GTACTTCACC AACGCGTGGT GTTGGCTCGA TTTCGTGATT 
GTCATGCTGT CGCTAATTAA TTTGGCCGCG GTCTGGTCCG GGGCCGATGA TGTGCCCGCC 
TTTCGCTCGA TGCGAACTCT GCGCGCCCTG CGACCACTGC GGGCAGTTTC GCGCTGGGAG 
GGCATGAAAG TCGTCGTTAA TGCGCTGGTA CAAGCTATAC CGTCCATCTT CAATGTGCTA 
TTGGTGTGTC TAATATTTTG GCTAATTTTT GCCATAATGG GTGTACAGCT TTTTGCTGGA 
AAATATTTTA AGTGCGAGGA CATGAATGGC ACGAAGCTCA GCCACGAGAT CATACCAAAT 
CGCAATGCCT GCGAGAGCGA GAACTACACG TGGGTGAATT CAGCAATGAA TTTCGATCAT 
GTAGGTAACG CGTATCTGTG CCTTTTCCAA GTGGCCACCT TCAAAGGCTG GATACAAATC 
ATGAACGATG CTATCGATTC ACGAGAGGTG GACAAGCAAC CAATTCGTGA AACGAACATC 
TACATGTATT TATATTTCGT ATTCTTCATC ATATTTGGAT CATTTTTCAC ACTCAATCTG 
TTCATTGGTG TTATCATTGA TAATTTTAAT GAGCAAAAGA AAAAAGCAGG TGGATCATTA 
GAAATGTTCA TGACAGAAGA TCAGAAAAAG TACTATAATG CTATGAAAAA GATGGGCTCT 
AAAAAACCAT TAAAAGCCAT TCCAAGACCA AGGTGGCGAC CACAAGCAAT AGTCTTTGAA 
ATAGTAACCG ATAAGAAATT CGATATAATC ATTATGTTAT TCATTGGTCT GAACATGTTC 
ACCATGACCC TCGATCGTTA CGATGCGTCG GACACGTATA ACGCGGTCCT AGACTATCTC 
AATGCGATAT TCGTAGTTAT TTTCAGTTCC GAATGTCTAT TAAAAATATT CGCTTTACGA 
TATCACTATT TTATTGAGCC ATGGAATTTA TTTGATGTAG TAGTTGTCAT TTTATCCATC 
TTAGGTCTTG TACTTAGCGA TATTATCGAG AAGTACTTCG TGTCGCCGAC CCTGCTCCGA 
GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC CTTCGACTGG TGAAGGGAGC CAAGGGCATT 
CGGACACTGC TCTTCGCGTT GGCCATGTCG CTGCCGGCCC TGTTCAACAT CTGCCTGCTG 
CTGTTCCTGG TCATGTTCAT CTTTGCCATT TTCGGCATGT CGTTCTTCAT GCACGTGAAG 
GAGAAGAGCG GCATCAACGA CGTCTACAAC TTCAAGACCT TTGGCCAGAG CATGATCCTG 
CTCTTTCAGA TGTCGACGTC AGCCGGTTGG GATGGTGTAC TGGACGCCAT TATCAATGAG 
GAAGCATGCG ATCCACCCGA CAACGACAAA GGCTATCCGG GCAATTGTGG TTCAGCGACC 
GTTGGAATAA CGTTTCTCCT CTCATACCTA GTTATAAGCT TTTTGATAGT TATTAATATG 
TACATTGCTG TCATTCTCGA GAACTATAGT CAGGCCACCG AGGACGTGCA AGAGGGTCTA 
ACCGACGACG ACTACGACAT GTACTATGAG ATCTGGCAGC AATTCGATCC GGAGGGCACC 
CAGTACATAC GCTATGATCA GCTGTCCGAA TTCCTGGACG TACTGGAGCC CCCGCTGCAG 
ATCCACAAAC CGAACAAGTA CAAGATCATA TCGATGGACA TACCCATCTG TCGCGGTGAC 
CTCATGTACT GCGTCGACAT CCTCGACGCC CTTACGAAAG ACTTCTTTGC GCGGAAGGGC 
AATCCGATAG AGGAGACGGG TGAGATTGGT GAGATAGCGG CCCGCCCGGA TACGGAGGGC 
TACGAGCCCG TCTCATCAAC GCTGTGGCGT CAGCGTGAGG AGTACTGCGC CCGGCTAATC 
CAGCACGCCT GGCGAAAGCA CAAGGCGCGC GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT 
ACGGATCATG GCGATGGCGG TGATCCGGAT GCCGGGGACC CGGCGCCCGA TGAAGCAACG 
GACGGCGATG CGCCCGCTGG TGGAGATGGT AGTGTTAACG GTACTGCAGA AGGAGCTGCC 
GATGCCGATG AGAGTAATGT AAATAGTCCG GGTGAGGATG CAGCGGCGGC GGCAGCAGCA 
GCAGCAGCAG CGGCGGCGGC GGGCACGACG ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC 
GGGCGACAGA CCGCCGTTCT CGTGGAGAGC GACGGGTTCG TGACGAAGAA CGGCCACAAG 
GTGGTCATCC ACTCGCGATC GCCGAGCATC ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201680.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188609.1 Drosophila melanogaster paralytic (para), transcript variant N, mRNA.

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CloneID	OFa01811
Clone ID Related Accession (Same CDS sequence)	NM_001201680.1
Accession Version	NM_001201680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform N
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTATTCAA ACTGGCCAAG 
TCTTGGCCCA CACTTAATTT ACTCATTTCG ATTATGGGAC GCACCATGGG CGCTTTGGGT 
AATCTGACAT TTGTACTTTG CATTATCATC TTCATCTTTG CGGTGATGGG AATGCAACTG 
TTCGGAAAGA ATTATCATGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGGTGAGAGG 
ACCAACCAGA TCAGTTGGAT TTGGAGCGAA GAGCATGGTG ACAACGAACT GGAGCTGGGC 
CACGACGAGA TCCTCGCCGA CGGCCTCATC AAGAAGGGGA TCAAGGAGCA GACGCAACTG 
GAGGTGGCCA TCGGGGATGG CATGGAATTC ACGATACACG GCGACATGAA GAACAACAAG 
CCGAAGAAAT CCAAATATCT AAATAACGCA ACGGACGACG ACACTGCCAG CATTAACTCA 
TATGGTAGCC ATAAGAATCG ACCATTCAAG GACGAGAGCC ACAAGGGCAG CGCCGAGACG 
ATGGAGGGCG AGGAGAAGCG CGACGCCAGC AAGGAGGATT TAGGTCTCGA CGAGGAACTG 
GACGAGGAGG GCGAATGCGA GGAGGGCCCG CTCGACGGTG ATATCATTAT TCATGCACAC 
GACGAGGATA TACTCGATGA ATATCCAGCT GATTGCTGCC CCGATTCGTA CTATAAGAAA 
TTTCCGATCT TAGCCGGTGA CGATGACTCG CCGTTCTGGC AAGGATGGGG CAATTTACGA 
CTGAAAACTT TTCAATTAAT TGAAAATAAA TATTTTGAAA CAGCTGTTAT CACTATGATT 
TTAATGAGTA GCTTAGCTTT GGCATTAGAA GATGTACATC TGCCACAAAG ACCCATACTG 
CAGGATATTT TATACTATAT GGACAGAATA TTTACGGTTA TATTCTTCTT GGAAATGTTA 
ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG TACTTCACCA ACGCGTGGTG TTGGCTCGAT 
TTCGTGATTG TCATGGTATC GCTTATCAAC TTCGTTGCTT CACTTGTTGG AGCTGGTGGT 
ATTCAAGCCT TCAAGACTAT GCGAACGTTA AGAGCACTGA GACCACTACG TGCCATGTCC 
CGTATGCAGG GCATGAGGGT CGTCGTTAAT GCGCTGGTAC AAGCTATACC GTCCATCTTC 
AATGTGCTAT TGGTGTGTCT AATATTTTGG CTAATTTTTG CCATAATGGG TGTACAGCTT 
TTTGCTGGAA AATATTTTAA GTGCGAGGAC ATGAATGGCA CGAAGCTCAG CCACGAGATC 
ATACCAAATC GCAATGCCTG CGAGAGCGAG AACTACACGT GGGTGAATTC AGCAATGAAT 
TTCGATCATG TAGGTAACGC GTATCTGTGC CTTTTCCAAG TGGCCACCTT CAAAGGCTGG 
ATACAAATCA TGAACGATGC TATCGATTCA CGAGAGGTGG ACAAGCAACC AATTCGTGAA 
ACGAACATCT ACATGTATTT ATATTTCGTA TTCTTCATCA TATTTGGATC ATTTTTCACA 
CTCAATCTGT TCATTGGTGT TATCATTGAT AATTTTAATG AGCAAAAGAA AAAAGCAGGT 
GGATCATTAG AAATGTTCAT GACAGAAGAT CAGAAAAAGT ACTATAATGC TATGAAAAAG 
ATGGGCTCTA AAAAACCATT AAAAGCCATT CCAAGACCAA GGTGGCGACC ACAAGCAATA 
GTCTTTGAAA TAGTAACCGA TAAGAAATTC GATATAATCA TTATGTTATT CATTGGTCTG 
AACATGTTCA CCATGACCCT CGATCGTTAC GATGCGTCGG ACACGTATAA CGCGGTCCTA 
GACTATCTCA ATGCGATATT CGTAGTTATT TTCAGTTCCG AATGTCTATT AAAAATATTC 
GCTTTACGAT ATCACTATTT TATTGAGCCA TGGAATTTAT TTGATGTAGT AGTTGTCATT 
TTATCCATCT TAGGTCTTGT ACTTAGCGAT ATTATCGAGA AGTACTTCGT GTCGCCGACC 
CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG GGCCGTGTCC TTCGACTGGT GAAGGGAGCC 
AAGGGCATTC GGACACTGCT CTTCGCGTTG GCCATGTCGC TGCCGGCCCT GTTCAACATC 
TGCCTGCTGC TGTTCCTGGT CATGTTCATC TTTGCCATTT TCGGCATGTC GTTCTTCATG 
CACGTGAAGG AGAAGAGCGG CATCAACGAC GTCTACAACT TCAAGACCTT TGGCCAGAGC 
ATGATCCTGC TCTTTCAGAT GTCGACGTCA GCCGGTTGGG ATGGTGTACT GGACGCCATT 
ATCAATGAGG AAGCATGCGA TCCACCCGAC AACGACAAAG GCTATCCGGG CAATTGTGGT 
TCAGCGACCG TTGGAATAAC GTTTCTCCTC TCATACCTAG TTATAAGCTT TTTGATAGTT 
ATTAATATGT ACATTGCTGT CATTCTCGAG AACTATAGTC AGGCCACCGA GGACGTGCAA 
GAGGGTCTAA CCGACGACGA CTACGACATG TACTATGAGA TCTGGCAGCA ATTCGATCCG 
GAGGGCACCC AGTACATACG CTATGATCAG CTGTCCGAAT TCCTGGACGT ACTGGAGCCC 
CCGCTGCAGA TCCACAAACC GAACAAGTAC AAGATCATAT CGATGGACAT ACCCATCTGT 
CGCGGTGACC TCATGTACTG CGTCGACATC CTCGACGCCC TTACGAAAGA CTTCTTTGCG 
CGGAAGGGCA ATCCGATAGA GGAGACGGGT GAGATTGGTG AGATAGCGGC CCGCCCGGAT 
ACGGAGGGCT ACGAGCCCGT CTCATCAACG CTGTGGCGTC AGCGTGAGGA GTACTGCGCC 
CGGCTAATCC AGCACGCCTG GCGAAAGCAC AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT 
GAGCCGGATA CGGATCATGG CGATGGCGGT GATCCGGATG CCGGGGACCC GGCGCCCGAT 
GAAGCAACGG ACGGCGATGC GCCCGCTGGT GGAGATGGTA GTGTTAACGG TACTGCAGAA 
GGAGCTGCCG ATGCCGATGA GAGTAATGTA AATAGTCCGG GTGAGGATGC AGCGGCGGCG 
GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG GGCACGACGA CGGCGGGAAG TCCCGGAGCG 
GGTAGCGCCG GGCGACAGAC CGCCGTTCTC GTGGAGAGCG ACGGGTTCGT GACGAAGAAC 
GGCCACAAGG TGGTCATCCA CTCGCGATCG CCGAGCATCA CGTCGCGCAC GGCGGATGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188609.1
CDS	269..6571
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201680.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant N, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201680.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTATTCAA ACTGGCCAAG 
TCTTGGCCCA CACTTAATTT ACTCATTTCG ATTATGGGAC GCACCATGGG CGCTTTGGGT 
AATCTGACAT TTGTACTTTG CATTATCATC TTCATCTTTG CGGTGATGGG AATGCAACTG 
TTCGGAAAGA ATTATCATGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGGTGAGAGG 
ACCAACCAGA TCAGTTGGAT TTGGAGCGAA GAGCATGGTG ACAACGAACT GGAGCTGGGC 
CACGACGAGA TCCTCGCCGA CGGCCTCATC AAGAAGGGGA TCAAGGAGCA GACGCAACTG 
GAGGTGGCCA TCGGGGATGG CATGGAATTC ACGATACACG GCGACATGAA GAACAACAAG 
CCGAAGAAAT CCAAATATCT AAATAACGCA ACGGACGACG ACACTGCCAG CATTAACTCA 
TATGGTAGCC ATAAGAATCG ACCATTCAAG GACGAGAGCC ACAAGGGCAG CGCCGAGACG 
ATGGAGGGCG AGGAGAAGCG CGACGCCAGC AAGGAGGATT TAGGTCTCGA CGAGGAACTG 
GACGAGGAGG GCGAATGCGA GGAGGGCCCG CTCGACGGTG ATATCATTAT TCATGCACAC 
GACGAGGATA TACTCGATGA ATATCCAGCT GATTGCTGCC CCGATTCGTA CTATAAGAAA 
TTTCCGATCT TAGCCGGTGA CGATGACTCG CCGTTCTGGC AAGGATGGGG CAATTTACGA 
CTGAAAACTT TTCAATTAAT TGAAAATAAA TATTTTGAAA CAGCTGTTAT CACTATGATT 
TTAATGAGTA GCTTAGCTTT GGCATTAGAA GATGTACATC TGCCACAAAG ACCCATACTG 
CAGGATATTT TATACTATAT GGACAGAATA TTTACGGTTA TATTCTTCTT GGAAATGTTA 
ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG TACTTCACCA ACGCGTGGTG TTGGCTCGAT 
TTCGTGATTG TCATGGTATC GCTTATCAAC TTCGTTGCTT CACTTGTTGG AGCTGGTGGT 
ATTCAAGCCT TCAAGACTAT GCGAACGTTA AGAGCACTGA GACCACTACG TGCCATGTCC 
CGTATGCAGG GCATGAGGGT CGTCGTTAAT GCGCTGGTAC AAGCTATACC GTCCATCTTC 
AATGTGCTAT TGGTGTGTCT AATATTTTGG CTAATTTTTG CCATAATGGG TGTACAGCTT 
TTTGCTGGAA AATATTTTAA GTGCGAGGAC ATGAATGGCA CGAAGCTCAG CCACGAGATC 
ATACCAAATC GCAATGCCTG CGAGAGCGAG AACTACACGT GGGTGAATTC AGCAATGAAT 
TTCGATCATG TAGGTAACGC GTATCTGTGC CTTTTCCAAG TGGCCACCTT CAAAGGCTGG 
ATACAAATCA TGAACGATGC TATCGATTCA CGAGAGGTGG ACAAGCAACC AATTCGTGAA 
ACGAACATCT ACATGTATTT ATATTTCGTA TTCTTCATCA TATTTGGATC ATTTTTCACA 
CTCAATCTGT TCATTGGTGT TATCATTGAT AATTTTAATG AGCAAAAGAA AAAAGCAGGT 
GGATCATTAG AAATGTTCAT GACAGAAGAT CAGAAAAAGT ACTATAATGC TATGAAAAAG 
ATGGGCTCTA AAAAACCATT AAAAGCCATT CCAAGACCAA GGTGGCGACC ACAAGCAATA 
GTCTTTGAAA TAGTAACCGA TAAGAAATTC GATATAATCA TTATGTTATT CATTGGTCTG 
AACATGTTCA CCATGACCCT CGATCGTTAC GATGCGTCGG ACACGTATAA CGCGGTCCTA 
GACTATCTCA ATGCGATATT CGTAGTTATT TTCAGTTCCG AATGTCTATT AAAAATATTC 
GCTTTACGAT ATCACTATTT TATTGAGCCA TGGAATTTAT TTGATGTAGT AGTTGTCATT 
TTATCCATCT TAGGTCTTGT ACTTAGCGAT ATTATCGAGA AGTACTTCGT GTCGCCGACC 
CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG GGCCGTGTCC TTCGACTGGT GAAGGGAGCC 
AAGGGCATTC GGACACTGCT CTTCGCGTTG GCCATGTCGC TGCCGGCCCT GTTCAACATC 
TGCCTGCTGC TGTTCCTGGT CATGTTCATC TTTGCCATTT TCGGCATGTC GTTCTTCATG 
CACGTGAAGG AGAAGAGCGG CATCAACGAC GTCTACAACT TCAAGACCTT TGGCCAGAGC 
ATGATCCTGC TCTTTCAGAT GTCGACGTCA GCCGGTTGGG ATGGTGTACT GGACGCCATT 
ATCAATGAGG AAGCATGCGA TCCACCCGAC AACGACAAAG GCTATCCGGG CAATTGTGGT 
TCAGCGACCG TTGGAATAAC GTTTCTCCTC TCATACCTAG TTATAAGCTT TTTGATAGTT 
ATTAATATGT ACATTGCTGT CATTCTCGAG AACTATAGTC AGGCCACCGA GGACGTGCAA 
GAGGGTCTAA CCGACGACGA CTACGACATG TACTATGAGA TCTGGCAGCA ATTCGATCCG 
GAGGGCACCC AGTACATACG CTATGATCAG CTGTCCGAAT TCCTGGACGT ACTGGAGCCC 
CCGCTGCAGA TCCACAAACC GAACAAGTAC AAGATCATAT CGATGGACAT ACCCATCTGT 
CGCGGTGACC TCATGTACTG CGTCGACATC CTCGACGCCC TTACGAAAGA CTTCTTTGCG 
CGGAAGGGCA ATCCGATAGA GGAGACGGGT GAGATTGGTG AGATAGCGGC CCGCCCGGAT 
ACGGAGGGCT ACGAGCCCGT CTCATCAACG CTGTGGCGTC AGCGTGAGGA GTACTGCGCC 
CGGCTAATCC AGCACGCCTG GCGAAAGCAC AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT 
GAGCCGGATA CGGATCATGG CGATGGCGGT GATCCGGATG CCGGGGACCC GGCGCCCGAT 
GAAGCAACGG ACGGCGATGC GCCCGCTGGT GGAGATGGTA GTGTTAACGG TACTGCAGAA 
GGAGCTGCCG ATGCCGATGA GAGTAATGTA AATAGTCCGG GTGAGGATGC AGCGGCGGCG 
GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG GGCACGACGA CGGCGGGAAG TCCCGGAGCG 
GGTAGCGCCG GGCGACAGAC CGCCGTTCTC GTGGAGAGCG ACGGGTTCGT GACGAAGAAC 
GGCCACAAGG TGGTCATCCA CTCGCGATCG CCGAGCATCA CGTCGCGCAC GGCGGATGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201678.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188607.1 Drosophila melanogaster paralytic (para), transcript variant L, mRNA.

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CloneID	OFa01812
Clone ID Related Accession (Same CDS sequence)	NM_001201678.1
Accession Version	NM_001201678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTTTTCAA GCTGGCCAAA 
TCGTGGCCGA CGCTGAATTT GCTCATATCG ATTATGGGCC GCACTGTCGG CGCCCTCGGC 
AATCTGACCT TCGTCCTGTG CATTATTATC TTCATATTCG CGGTCATGGG CATGCAGCTG 
TTTGGCAAGA ACTACACAGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGGACGAC 
GACACTGCCA GCATTAACTC ATATGGTAGC CATAAGAATC GACCATTCAA GGACGAGAGC 
CACAAGGGCA GCGCCGAGAC GATGGAGGGC GAGGAGAAGC GCGACGCCAG CAAGGAGGAT 
TTAGGTCTCG ACGAGGAACT GGACGAGGAG GGCGAATGCG AGGAGGGCCC GCTCGACGGT 
GATATCATTA TTCATGCACA CGACGAGGAT ATACTCGATG AATATCCAGC TGATTGCTGC 
CCCGATTCGT ACTATAAGAA ATTTCCGATC TTAGCCGGTG ACGATGACTC GCCGTTCTGG 
CAAGGATGGG GCAATTTACG ACTGAAAACT TTTCAATTAA TTGAAAATAA ATATTTTGAA 
ACAGCTGTTA TCACTATGAT TTTAATGAGT AGCTTAGCTT TGGCATTAGA AGATGTACAT 
CTGCCACAAA GACCCATACT GCAGGATATT TTATACTATA TGGACAGAAT ATTTACGGTT 
ATATTCTTCT TGGAAATGTT AATCAAGTGG TTGGCGCTCG GCTTCAAAGT GTACTTCACC 
AACGCGTGGT GTTGGCTCGA TTTCGTGATT GTCATGGTAT CGCTTATCAA CTTCGTTGCT 
TCACTTGTTG GAGCTGGTGG TATTCAAGCC TTCAAGACTA TGCGAACGTT AAGAGCACTG 
AGACCACTAC GTGCCATGTC CCGTATGCAG GGCATGAGGG TCGTCGTTAA TGCGCTGGTA 
CAAGCTATAC CGTCCATCTT CAATGTGCTA TTGGTGTGTC TAATATTTTG GCTAATTTTT 
GCCATAATGG GTGTACAGCT TTTTGCTGGA AAATATTTTA AGTGCGAGGA CATGAATGGC 
ACGAAGCTCA GCCACGAGAT CATACCAAAT CGCAATGCCT GCGAGAGCGA GAACTACACG 
TGGGTGAATT CAGCAATGAA TTTCGATCAT GTAGGTAACG CGTATCTGTG CCTTTTCCAA 
GTGGCCACCT TCAAAGGCTG GATACAAATC ATGAACGATG CTATCGATTC ACGAGAGGTG 
GACAAGCAAC CAATTCGTGA AACGAACATC TACATGTATT TATATTTCGT ATTCTTCATC 
ATATTTGGAT CATTTTTCAC ACTCAATCTG TTCATTGGTG TTATCATTGA TAATTTTAAT 
GAGCAAAAGA AAAAAGCAGG TGGATCATTA GAAATGTTCA TGACAGAAGA TCAGAAAAAG 
TACTATAATG CTATGAAAAA GATGGGCTCT AAAAAACCAT TAAAAGCCAT TCCAAGACCA 
AGGTGGCGAC CACAAGCAAT AGTCTTTGAA ATAGTAACCG ATAAGAAATT CGATATAATC 
ATTATGTTAT TCATTGGTCT GAACATGTTC ACCATGACCC TCGATCGTTA CGATGCGTCG 
GACACGTATA ACGCGGTCCT AGACTATCTC AATGCGATAT TCGTAGTTAT TTTCAGTTCC 
GAATGTCTAT TAAAAATATT CGCTTTACGA TATCACTATT TTATTGAGCC ATGGAATTTA 
TTTGATGTAG TAGTTGTCAT TTTATCCATC TTAGGTCTTG TACTTAGCGA TATTATCGAG 
AAGTACTTCG TGTCGCCGAC CCTGCTCCGA GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC 
CTTCGACTGG TGAAGGGAGC CAAGGGCATT CGGACACTGC TCTTCGCGTT GGCCATGTCG 
CTGCCGGCCC TGTTCAACAT CTGCCTGCTG CTGTTCCTGG TCATGTTCAT CTTTGCCATT 
TTCGGCATGT CGTTCTTCAT GCACGTGAAG GAGAAGAGCG GCATCAACGA CGTCTACAAC 
TTCAAGACCT TTGGCCAGAG CATGATCCTG CTCTTTCAGA TGTCGACGTC AGCCGGTTGG 
GATGGTGTAC TGGACGCCAT TATCAATGAG GAAGCATGCG ATCCACCCGA CAACGACAAA 
GGCTATCCGG GCAATTGTGG TTCAGCGACC GTTGGAATAA CGTTTCTCCT CTCATACCTA 
GTTATAAGCT TTTTGATAGT TATTAATATG TACATTGCTG TCATTCTCGA GAACTATAGT 
CAGGCCACCG AGGACGTGCA AGAGGGTCTA ACCGACGACG ACTACGACAT GTACTATGAG 
ATCTGGCAGC AATTCGATCC GGAGGGCACC CAGTACATAC GCTATGATCA GCTGTCCGAA 
TTCCTGGACG TACTGGAGCC CCCGCTGCAG ATCCACAAAC CGAACAAGTA CAAGATCATA 
TCGATGGACA TACCCATCTG TCGCGGTGAC CTCATGTACT GCGTCGACAT CCTCGACGCC 
CTTACGAAAG ACTTCTTTGC GCGGAAGGGC AATCCGATAG AGGAGACGGG TGAGATTGGT 
GAGATAGCGG CCCGCCCGGA TACGGAGGGC TACGAGCCCG TCTCATCAAC GCTGTGGCGT 
CAGCGTGAGG AGTACTGCGC CCGGCTAATC CAGCACGCCT GGCGAAAGCA CAAGGCGCGC 
GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT ACGGATCATG GCGATGGCGG TGATCCGGAT 
GCCGGGGACC CGGCGCCCGA TGAAGCAACG GACGGCGATG CGCCCGCTGG TGGAGATGGT 
AGTGTTAACG GTACTGCAGA AGGAGCTGCC GATGCCGATG AGAGTAATGT AAATAGTCCG 
GGTGAGGATG CAGCGGCGGC GGCAGCAGCA GCAGCAGCAG CGGCGGCGGC GGGCACGACG 
ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC GGGCGACAGA CCGCCGTTCT CGTGGAGAGC 
GACGGGTTCG TGACGAAGAA CGGCCACAAG GTGGTCATCC ACTCGCGATC GCCGAGCATC 
ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188607.1
CDS	269..6532
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201678.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant L, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201678.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTTTTCAA GCTGGCCAAA 
TCGTGGCCGA CGCTGAATTT GCTCATATCG ATTATGGGCC GCACTGTCGG CGCCCTCGGC 
AATCTGACCT TCGTCCTGTG CATTATTATC TTCATATTCG CGGTCATGGG CATGCAGCTG 
TTTGGCAAGA ACTACACAGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGGACGAC 
GACACTGCCA GCATTAACTC ATATGGTAGC CATAAGAATC GACCATTCAA GGACGAGAGC 
CACAAGGGCA GCGCCGAGAC GATGGAGGGC GAGGAGAAGC GCGACGCCAG CAAGGAGGAT 
TTAGGTCTCG ACGAGGAACT GGACGAGGAG GGCGAATGCG AGGAGGGCCC GCTCGACGGT 
GATATCATTA TTCATGCACA CGACGAGGAT ATACTCGATG AATATCCAGC TGATTGCTGC 
CCCGATTCGT ACTATAAGAA ATTTCCGATC TTAGCCGGTG ACGATGACTC GCCGTTCTGG 
CAAGGATGGG GCAATTTACG ACTGAAAACT TTTCAATTAA TTGAAAATAA ATATTTTGAA 
ACAGCTGTTA TCACTATGAT TTTAATGAGT AGCTTAGCTT TGGCATTAGA AGATGTACAT 
CTGCCACAAA GACCCATACT GCAGGATATT TTATACTATA TGGACAGAAT ATTTACGGTT 
ATATTCTTCT TGGAAATGTT AATCAAGTGG TTGGCGCTCG GCTTCAAAGT GTACTTCACC 
AACGCGTGGT GTTGGCTCGA TTTCGTGATT GTCATGGTAT CGCTTATCAA CTTCGTTGCT 
TCACTTGTTG GAGCTGGTGG TATTCAAGCC TTCAAGACTA TGCGAACGTT AAGAGCACTG 
AGACCACTAC GTGCCATGTC CCGTATGCAG GGCATGAGGG TCGTCGTTAA TGCGCTGGTA 
CAAGCTATAC CGTCCATCTT CAATGTGCTA TTGGTGTGTC TAATATTTTG GCTAATTTTT 
GCCATAATGG GTGTACAGCT TTTTGCTGGA AAATATTTTA AGTGCGAGGA CATGAATGGC 
ACGAAGCTCA GCCACGAGAT CATACCAAAT CGCAATGCCT GCGAGAGCGA GAACTACACG 
TGGGTGAATT CAGCAATGAA TTTCGATCAT GTAGGTAACG CGTATCTGTG CCTTTTCCAA 
GTGGCCACCT TCAAAGGCTG GATACAAATC ATGAACGATG CTATCGATTC ACGAGAGGTG 
GACAAGCAAC CAATTCGTGA AACGAACATC TACATGTATT TATATTTCGT ATTCTTCATC 
ATATTTGGAT CATTTTTCAC ACTCAATCTG TTCATTGGTG TTATCATTGA TAATTTTAAT 
GAGCAAAAGA AAAAAGCAGG TGGATCATTA GAAATGTTCA TGACAGAAGA TCAGAAAAAG 
TACTATAATG CTATGAAAAA GATGGGCTCT AAAAAACCAT TAAAAGCCAT TCCAAGACCA 
AGGTGGCGAC CACAAGCAAT AGTCTTTGAA ATAGTAACCG ATAAGAAATT CGATATAATC 
ATTATGTTAT TCATTGGTCT GAACATGTTC ACCATGACCC TCGATCGTTA CGATGCGTCG 
GACACGTATA ACGCGGTCCT AGACTATCTC AATGCGATAT TCGTAGTTAT TTTCAGTTCC 
GAATGTCTAT TAAAAATATT CGCTTTACGA TATCACTATT TTATTGAGCC ATGGAATTTA 
TTTGATGTAG TAGTTGTCAT TTTATCCATC TTAGGTCTTG TACTTAGCGA TATTATCGAG 
AAGTACTTCG TGTCGCCGAC CCTGCTCCGA GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC 
CTTCGACTGG TGAAGGGAGC CAAGGGCATT CGGACACTGC TCTTCGCGTT GGCCATGTCG 
CTGCCGGCCC TGTTCAACAT CTGCCTGCTG CTGTTCCTGG TCATGTTCAT CTTTGCCATT 
TTCGGCATGT CGTTCTTCAT GCACGTGAAG GAGAAGAGCG GCATCAACGA CGTCTACAAC 
TTCAAGACCT TTGGCCAGAG CATGATCCTG CTCTTTCAGA TGTCGACGTC AGCCGGTTGG 
GATGGTGTAC TGGACGCCAT TATCAATGAG GAAGCATGCG ATCCACCCGA CAACGACAAA 
GGCTATCCGG GCAATTGTGG TTCAGCGACC GTTGGAATAA CGTTTCTCCT CTCATACCTA 
GTTATAAGCT TTTTGATAGT TATTAATATG TACATTGCTG TCATTCTCGA GAACTATAGT 
CAGGCCACCG AGGACGTGCA AGAGGGTCTA ACCGACGACG ACTACGACAT GTACTATGAG 
ATCTGGCAGC AATTCGATCC GGAGGGCACC CAGTACATAC GCTATGATCA GCTGTCCGAA 
TTCCTGGACG TACTGGAGCC CCCGCTGCAG ATCCACAAAC CGAACAAGTA CAAGATCATA 
TCGATGGACA TACCCATCTG TCGCGGTGAC CTCATGTACT GCGTCGACAT CCTCGACGCC 
CTTACGAAAG ACTTCTTTGC GCGGAAGGGC AATCCGATAG AGGAGACGGG TGAGATTGGT 
GAGATAGCGG CCCGCCCGGA TACGGAGGGC TACGAGCCCG TCTCATCAAC GCTGTGGCGT 
CAGCGTGAGG AGTACTGCGC CCGGCTAATC CAGCACGCCT GGCGAAAGCA CAAGGCGCGC 
GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT ACGGATCATG GCGATGGCGG TGATCCGGAT 
GCCGGGGACC CGGCGCCCGA TGAAGCAACG GACGGCGATG CGCCCGCTGG TGGAGATGGT 
AGTGTTAACG GTACTGCAGA AGGAGCTGCC GATGCCGATG AGAGTAATGT AAATAGTCCG 
GGTGAGGATG CAGCGGCGGC GGCAGCAGCA GCAGCAGCAG CGGCGGCGGC GGGCACGACG 
ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC GGGCGACAGA CCGCCGTTCT CGTGGAGAGC 
GACGGGTTCG TGACGAAGAA CGGCCACAAG GTGGTCATCC ACTCGCGATC GCCGAGCATC 
ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201685.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188614.1 Drosophila melanogaster paralytic (para), transcript variant S, mRNA.

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CloneID	OFa01813
Clone ID Related Accession (Same CDS sequence)	NM_001201685.1
Accession Version	NM_001201685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTTTTCAA GCTGGCCAAA 
TCGTGGCCGA CGCTGAATTT GCTCATATCG ATTATGGGCC GCACTGTCGG CGCCCTCGGC 
AATCTGACCT TCGTCCTGTG CATTATTATC TTCATATTCG CGGTCATGGG CATGCAGCTG 
TTTGGCAAGA ACTACACAGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGGTGAGAGG 
ACCAACCAGA TCAGTTGGAT TTGGAGCGAA GAGCATGGTG ACAACGAACT GGAGCTGGGC 
CACGACGAGA TCCTCGCCGA CGGCCTCATC AAGAAGGGGA TCAAGGAGCA GACGCAACTG 
GAGGTGGCCA TCGGGGATGG CATGGAATTC ACGATACACG GCGACATGAA GAACAACAAG 
CCGAAGAAAT CCAAATATCT AAATAACGCA ACGGACGACG ACACTGCCAG CATTAACTCA 
TATGGTAGCC ATAAGAATCG ACCATTCAAG GACGAGAGCC ACAAGGGCAG CGCCGAGACG 
ATGGAGGGCG AGGAGAAGCG CGACGCCAGC AAGGAGGATT TAGGTCTCGA CGAGGAACTG 
GACGAGGAGG GCGAATGCGA GGAGGGCCCG CTCGACGGTG ATATCATTAT TCATGCACAC 
GACGAGGATA TACTCGATGA ATATCCAGCT GATTGCTGCC CCGATTCGTA CTATAAGAAA 
TTTCCGATCT TAGCCGGTGA CGATGACTCG CCGTTCTGGC AAGGATGGGG CAATTTACGA 
CTGAAAACTT TTCAATTAAT TGAAAATAAA TATTTTGAAA CAGCTGTTAT CACTATGATT 
TTAATGAGTA GCTTAGCTTT GGCATTAGAA GATGTACATC TGCCACAAAG ACCCATACTG 
CAGGATATTT TATACTATAT GGACAGAATA TTTACGGTTA TATTCTTCTT GGAAATGTTA 
ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG TACTTCACCA ACGCGTGGTG TTGGCTCGAT 
TTCGTGATTG TCATGGTATC GCTTATCAAC TTCGTTGCTT CACTTGTTGG AGCTGGTGGT 
ATTCAAGCCT TCAAGACTAT GCGAACGTTA AGAGCACTGA GACCACTACG TGCCATGTCC 
CGTATGCAGG GCATGAGGGT CGTCGTTAAT GCGCTGGTAC AAGCTATACC GTCCATCTTC 
AATGTGCTAT TGGTGTGTCT AATATTTTGG CTAATTTTTG CCATAATGGG TGTACAGCTT 
TTTGCTGGAA AATATTTTAA GTGCGAGGAC ATGAATGGCA CGAAGCTCAG CCACGAGATC 
ATACCAAATC GCAATGCCTG CGAGAGCGAG AACTACACGT GGGTGAATTC AGCAATGAAT 
TTCGATCATG TAGGTAACGC GTATCTGTGC CTTTTCCAAG TGGCCACCTT CAAAGGCTGG 
ATACAAATCA TGAACGATGC TATCGATTCA CGAGAGGTGG ACAAGCAACC AATTCGTGAA 
ACGAACATCT ACATGTATTT ATATTTCGTA TTCTTCATCA TATTTGGATC ATTTTTCACA 
CTCAATCTGT TCATTGGTGT TATCATTGAT AATTTTAATG AGCAAAAGAA AAAAGCAGGT 
GGATCATTAG AAATGTTCAT GACAGAAGAT CAGAAAAAGT ACTATAATGC TATGAAAAAG 
ATGGGCTCTA AAAAACCATT AAAAGCCATT CCAAGACCAA GGTGGCGACC ACAAGCAATA 
GTCTTTGAAA TAGTAACCGA TAAGAAATTC GATATAATCA TTATGTTATT CATTGGTCTG 
AACATGTTCA CCATGACCCT CGATCGTTAC GATGCGTCGG ACACGTATAA CGCGGTCCTA 
GACTATCTCA ATGCGATATT CGTAGTTATT TTCAGTTCCG AATGTCTATT AAAAATATTC 
GCTTTACGAT ATCACTATTT TATTGAGCCA TGGAATTTAT TTGATGTAGT AGTTGTCATT 
TTATCCATCT TAGGTCTTGT ACTTAGCGAT ATTATCGAGA AGTACTTCGT GTCGCCGACC 
CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG GGCCGTGTCC TTCGACTGGT GAAGGGAGCC 
AAGGGCATTC GGACACTGCT CTTCGCGTTG GCCATGTCGC TGCCGGCCCT GTTCAACATC 
TGCCTGCTGC TGTTCCTGGT CATGTTCATC TTTGCCATTT TCGGCATGTC GTTCTTCATG 
CACGTGAAGG AGAAGAGCGG CATCAACGAC GTCTACAACT TCAAGACCTT TGGCCAGAGC 
ATGATCCTGC TCTTTCAGAT GTCGACGTCA GCCGGTTGGG ATGGTGTACT GGACGCCATT 
ATCAATGAGG AAGCATGCGA TCCACCCGAC AACGACAAAG GCTATCCGGG CAATTGTGGT 
TCAGCGACCG TTGGAATAAC GTTTCTCCTC TCATACCTAG TTATAAGCTT TTTGATAGTT 
ATTAATATGT ACATTGCTGT CATTCTCGAG AACTATAGTC AGGCCACCGA GGACGTGCAA 
GAGGGTCTAA CCGACGACGA CTACGACATG TACTATGAGA TCTGGCAGCA ATTCGATCCG 
GAGGGCACCC AGTACATACG CTATGATCAG CTGTCCGAAT TCCTGGACGT ACTGGAGCCC 
CCGCTGCAGA TCCACAAACC GAACAAGTAC AAGATCATAT CGATGGACAT ACCCATCTGT 
CGCGGTGACC TCATGTACTG CGTCGACATC CTCGACGCCC TTACGAAAGA CTTCTTTGCG 
CGGAAGGGCA ATCCGATAGA GGAGACGGGT GAGATTGGTG AGATAGCGGC CCGCCCGGAT 
ACGGAGGGCT ACGAGCCCGT CTCATCAACG CTGTGGCGTC AGCGTGAGGA GTACTGCGCC 
CGGCTAATCC AGCACGCCTG GCGAAAGCAC AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT 
GAGCCGGATA CGGATCATGG CGATGGCGGT GATCCGGATG CCGGGGACCC GGCGCCCGAT 
GAAGCAACGG ACGGCGATGC GCCCGCTGGT GGAGATGGTA GTGTTAACGG TACTGCAGAA 
GGAGCTGCCG ATGCCGATGA GAGTAATGTA AATAGTCCGG GTGAGGATGC AGCGGCGGCG 
GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG GGCACGACGA CGGCGGGAAG TCCCGGAGCG 
GGTAGCGCCG GGCGACAGAC CGCCGTTCTC GTGGAGAGCG ACGGGTTCGT GACGAAGAAC 
GGCCACAAGG TGGTCATCCA CTCGCGATCG CCGAGCATCA CGTCGCGCAC GGCGGATGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188614.1
CDS	269..6571
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201685.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant S, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201685.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGTCTCCG TTTACTATTT CCCAACAGAG GACGATGACG AGGATGGGCC GACGTTCAAA 
GACAAGGCAC TCGAAGTGAT CCTCAAAGGC ATCGATGTGT TTTGTGTGTG GGACTGTTGC 
TGGGTTTGGT TGAAATTTCA GGAGTGGGTA TCGCTCATCG TCTTCGATCC CTTCGTCGAG 
CTCTTCATCA CGCTGTGCAT TGTGGTCAAC ACGATGTTCA TGGCAATGGA TCACCACGAT 
ATGAACAAGG AGATGGAACG CGTGCTCAAG AGTGGCAACT ATTTCTTCAC CGCCACCTTT 
GCCATCGAGG CCACCATGAA GCTAATGGCC ATGAGCCCCA AGTACTATTT CCAGGAGGGC 
TGGAACATCT TCGACTTCAT TATCGTGGCC CTATCGCTAT TGGAACTGGG ACTCGAGGGT 
GTCCAGGGTC TGTCCGTATT GCGTTCCTTT CGATTGCTGC GTGTTTTCAA GCTGGCCAAA 
TCGTGGCCGA CGCTGAATTT GCTCATATCG ATTATGGGCC GCACTGTCGG CGCCCTCGGC 
AATCTGACCT TCGTCCTGTG CATTATTATC TTCATATTCG CGGTCATGGG CATGCAGCTG 
TTTGGCAAGA ACTACACAGA TCACAAGGAC CGCTTTCCGG ATGGCGACCT GCCGCGCTGG 
AACTTCACCG ACTTTATGCA CAGCTTCATG ATCGTGTTCC GGGTGCTCTG CGGAGAATGG 
ATCGAGTCCA TGTGGGACTG CATGTACGTG GGCGATGTCT CGTGCATTCC CTTCTTCTTG 
GCCACCGTTG TCATCGGCAA TCTTGTGGTA CTTAACCTTT TCTTAGCCTT GCTTTTGTCC 
AATTTTGGCT CATCTAGCTT ATCAGCGCCG ACTGCCGATA ACGATACGAA TAAAATAGCC 
GAGGCCTTCA ATCGAATTGG CCGATTTAAA AGTTGGGTTA AGCGTAATAT TGCTGATTGT 
TTCAAGTTAA TACGTAACAA ATTGACAAAT CAAATAAGTG ATCAACCATC AGGTGAGAGG 
ACCAACCAGA TCAGTTGGAT TTGGAGCGAA GAGCATGGTG ACAACGAACT GGAGCTGGGC 
CACGACGAGA TCCTCGCCGA CGGCCTCATC AAGAAGGGGA TCAAGGAGCA GACGCAACTG 
GAGGTGGCCA TCGGGGATGG CATGGAATTC ACGATACACG GCGACATGAA GAACAACAAG 
CCGAAGAAAT CCAAATATCT AAATAACGCA ACGGACGACG ACACTGCCAG CATTAACTCA 
TATGGTAGCC ATAAGAATCG ACCATTCAAG GACGAGAGCC ACAAGGGCAG CGCCGAGACG 
ATGGAGGGCG AGGAGAAGCG CGACGCCAGC AAGGAGGATT TAGGTCTCGA CGAGGAACTG 
GACGAGGAGG GCGAATGCGA GGAGGGCCCG CTCGACGGTG ATATCATTAT TCATGCACAC 
GACGAGGATA TACTCGATGA ATATCCAGCT GATTGCTGCC CCGATTCGTA CTATAAGAAA 
TTTCCGATCT TAGCCGGTGA CGATGACTCG CCGTTCTGGC AAGGATGGGG CAATTTACGA 
CTGAAAACTT TTCAATTAAT TGAAAATAAA TATTTTGAAA CAGCTGTTAT CACTATGATT 
TTAATGAGTA GCTTAGCTTT GGCATTAGAA GATGTACATC TGCCACAAAG ACCCATACTG 
CAGGATATTT TATACTATAT GGACAGAATA TTTACGGTTA TATTCTTCTT GGAAATGTTA 
ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG TACTTCACCA ACGCGTGGTG TTGGCTCGAT 
TTCGTGATTG TCATGGTATC GCTTATCAAC TTCGTTGCTT CACTTGTTGG AGCTGGTGGT 
ATTCAAGCCT TCAAGACTAT GCGAACGTTA AGAGCACTGA GACCACTACG TGCCATGTCC 
CGTATGCAGG GCATGAGGGT CGTCGTTAAT GCGCTGGTAC AAGCTATACC GTCCATCTTC 
AATGTGCTAT TGGTGTGTCT AATATTTTGG CTAATTTTTG CCATAATGGG TGTACAGCTT 
TTTGCTGGAA AATATTTTAA GTGCGAGGAC ATGAATGGCA CGAAGCTCAG CCACGAGATC 
ATACCAAATC GCAATGCCTG CGAGAGCGAG AACTACACGT GGGTGAATTC AGCAATGAAT 
TTCGATCATG TAGGTAACGC GTATCTGTGC CTTTTCCAAG TGGCCACCTT CAAAGGCTGG 
ATACAAATCA TGAACGATGC TATCGATTCA CGAGAGGTGG ACAAGCAACC AATTCGTGAA 
ACGAACATCT ACATGTATTT ATATTTCGTA TTCTTCATCA TATTTGGATC ATTTTTCACA 
CTCAATCTGT TCATTGGTGT TATCATTGAT AATTTTAATG AGCAAAAGAA AAAAGCAGGT 
GGATCATTAG AAATGTTCAT GACAGAAGAT CAGAAAAAGT ACTATAATGC TATGAAAAAG 
ATGGGCTCTA AAAAACCATT AAAAGCCATT CCAAGACCAA GGTGGCGACC ACAAGCAATA 
GTCTTTGAAA TAGTAACCGA TAAGAAATTC GATATAATCA TTATGTTATT CATTGGTCTG 
AACATGTTCA CCATGACCCT CGATCGTTAC GATGCGTCGG ACACGTATAA CGCGGTCCTA 
GACTATCTCA ATGCGATATT CGTAGTTATT TTCAGTTCCG AATGTCTATT AAAAATATTC 
GCTTTACGAT ATCACTATTT TATTGAGCCA TGGAATTTAT TTGATGTAGT AGTTGTCATT 
TTATCCATCT TAGGTCTTGT ACTTAGCGAT ATTATCGAGA AGTACTTCGT GTCGCCGACC 
CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG GGCCGTGTCC TTCGACTGGT GAAGGGAGCC 
AAGGGCATTC GGACACTGCT CTTCGCGTTG GCCATGTCGC TGCCGGCCCT GTTCAACATC 
TGCCTGCTGC TGTTCCTGGT CATGTTCATC TTTGCCATTT TCGGCATGTC GTTCTTCATG 
CACGTGAAGG AGAAGAGCGG CATCAACGAC GTCTACAACT TCAAGACCTT TGGCCAGAGC 
ATGATCCTGC TCTTTCAGAT GTCGACGTCA GCCGGTTGGG ATGGTGTACT GGACGCCATT 
ATCAATGAGG AAGCATGCGA TCCACCCGAC AACGACAAAG GCTATCCGGG CAATTGTGGT 
TCAGCGACCG TTGGAATAAC GTTTCTCCTC TCATACCTAG TTATAAGCTT TTTGATAGTT 
ATTAATATGT ACATTGCTGT CATTCTCGAG AACTATAGTC AGGCCACCGA GGACGTGCAA 
GAGGGTCTAA CCGACGACGA CTACGACATG TACTATGAGA TCTGGCAGCA ATTCGATCCG 
GAGGGCACCC AGTACATACG CTATGATCAG CTGTCCGAAT TCCTGGACGT ACTGGAGCCC 
CCGCTGCAGA TCCACAAACC GAACAAGTAC AAGATCATAT CGATGGACAT ACCCATCTGT 
CGCGGTGACC TCATGTACTG CGTCGACATC CTCGACGCCC TTACGAAAGA CTTCTTTGCG 
CGGAAGGGCA ATCCGATAGA GGAGACGGGT GAGATTGGTG AGATAGCGGC CCGCCCGGAT 
ACGGAGGGCT ACGAGCCCGT CTCATCAACG CTGTGGCGTC AGCGTGAGGA GTACTGCGCC 
CGGCTAATCC AGCACGCCTG GCGAAAGCAC AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT 
GAGCCGGATA CGGATCATGG CGATGGCGGT GATCCGGATG CCGGGGACCC GGCGCCCGAT 
GAAGCAACGG ACGGCGATGC GCCCGCTGGT GGAGATGGTA GTGTTAACGG TACTGCAGAA 
GGAGCTGCCG ATGCCGATGA GAGTAATGTA AATAGTCCGG GTGAGGATGC AGCGGCGGCG 
GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG GGCACGACGA CGGCGGGAAG TCCCGGAGCG 
GGTAGCGCCG GGCGACAGAC CGCCGTTCTC GTGGAGAGCG ACGGGTTCGT GACGAAGAAC 
GGCCACAAGG TGGTCATCCA CTCGCGATCG CCGAGCATCA CGTCGCGCAC GGCGGATGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201679.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188608.1 Drosophila melanogaster paralytic (para), transcript variant M, mRNA.

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CloneID	OFa01814
Clone ID Related Accession (Same CDS sequence)	NM_001201679.1
Accession Version	NM_001201679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTATTCAAA CTGGCCAAGT CTTGGCCCAC ACTTAATTTA 
CTCATTTCGA TTATGGGACG CACCATGGGC GCTTTGGGTA ATCTGACATT TGTACTTTGC 
ATTATCATCT TCATCTTTGC GGTGATGGGA ATGCAACTGT TCGGAAAGAA TTATCATGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GAGCATGGTG ACAACGAACT GGAGCTGGGC 
CACGACGAGA TCCTCGCCGA CGGCCTCATC AAGAAGGGGA TCAAGGAGCA GACGCAACTG 
GAGGTGGCCA TCGGGGATGG CATGGAATTC ACGATACACG GCGACATGAA GAACAACAAG 
CCGAAGAAAT CCAAATATCT AAATAACGCA ACGGACGACG ACACTGCCAG CATTAACTCA 
TATGGTAGCC ATAAGAATCG ACCATTCAAG GACGAGAGCC ACAAGGGCAG CGCCGAGACG 
ATGGAGGGCG AGGAGAAGCG CGACGCCAGC AAGGAGGATT TAGGTCTCGA CGAGGAACTG 
GACGAGGAGG GCGAATGCGA GGAGGGCCCG CTCGACGGTG ATATCATTAT TCATGCACAC 
GACGAGGATA TACTCGATGA ATATCCAGCT GATTGCTGCC CCGATTCGTA CTATAAGAAA 
TTTCCGATCT TAGCCGGTGA CGATGACTCG CCGTTCTGGC AAGGATGGGG CAATTTACGA 
CTGAAAACTT TTCAATTAAT TGAAAATAAA TATTTTGAAA CAGCTGTTAT CACTATGATT 
TTAATGAGTA GCTTAGCTTT GGCATTAGAA GATGTACATC TGCCACAAAG ACCCATACTG 
CAGGATATTT TATACTATAT GGACAGAATA TTTACGGTTA TATTCTTCTT GGAAATGTTA 
ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG TACTTCACCA ACGCGTGGTG TTGGCTCGAT 
TTCGTGATTG TCATGGTATC GCTTATCAAC TTCGTTGCTT CACTTGTTGG AGCTGGTGGT 
ATTCAAGCCT TCAAGACTAT GCGAACGTTA AGAGCACTGA GACCACTACG TGCCATGTCC 
CGTATGCAGG GCATGAGGGT CGTCGTTAAT GCGCTGGTAC AAGCTATACC GTCCATCTTC 
AATGTGCTAT TGGTGTGTCT AATATTTTGG CTAATTTTTG CCATAATGGG TGTACAGCTT 
TTTGCTGGAA AATATTTTAA GTGCGAGGAC ATGAATGGCA CGAAGCTCAG CCACGAGATC 
ATACCAAATC GCAATGCCTG CGAGAGCGAG AACTACACGT GGGTGAATTC AGCAATGAAT 
TTCGATCATG TAGGTAACGC GTATCTGTGC CTTTTCCAAG TGGCCACCTT CAAAGGCTGG 
ATACAAATCA TGAACGATGC TATCGATTCA CGAGAGGTGG ACAAGCAACC AATTCGTGAA 
ACGAACATCT ACATGTATTT ATATTTCGTA TTCTTCATCA TATTTGGATC ATTTTTCACA 
CTCAATCTGT TCATTGGTGT TATCATTGAT AATTTTAATG AGCAAAAGAA AAAAGCAGGT 
GGATCATTAG AAATGTTCAT GACAGAAGAT CAGAAAAAGT ACTATAATGC TATGAAAAAG 
ATGGGCTCTA AAAAACCATT AAAAGCCATT CCAAGACCAA GGTGGCGACC ACAAGCAATA 
GTCTTTGAAA TAGTAACCGA TAAGAAATTC GATATAATCA TTATGTTATT CATTGGTCTG 
AACATGTTCA CCATGACCCT CGATCGTTAC GATGCGTCGG ACACGTATAA CGCGGTCCTA 
GACTATCTCA ATGCGATATT CGTAGTTATT TTCAGTTCCG AATGTCTATT AAAAATATTC 
GCTTTACGAT ATCACTATTT TATTGAGCCA TGGAATTTAT TTGATGTAGT AGTTGTCATT 
TTATCCATCT TAGGTCTTGT ACTTAGCGAT ATTATCGAGA AGTACTTCGT GTCGCCGACC 
CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG GGCCGTGTCC TTCGACTGGT GAAGGGAGCC 
AAGGGCATTC GGACACTGCT CTTCGCGTTG GCCATGTCGC TGCCGGCCCT GTTCAACATC 
TGCCTGCTGC TGTTCCTGGT CATGTTCATC TTTGCCATTT TCGGCATGTC GTTCTTCATG 
CACGTGAAGG AGAAGAGCGG CATCAACGAC GTCTACAACT TCAAGACCTT TGGCCAGAGC 
ATGATCCTGC TCTTTCAGAT GTCGACGTCA GCCGGTTGGG ATGGTGTACT GGACGCCATT 
ATCAATGAGG AAGCATGCGA TCCACCCGAC AACGACAAAG GCTATCCGGG CAATTGTGGT 
TCAGCGACCG TTGGAATAAC GTTTCTCCTC TCATACCTAG TTATAAGCTT TTTGATAGTT 
ATTAATATGT ACATTGCTGT CATTCTCGAG AACTATAGTC AGGCCACCGA GGACGTGCAA 
GAGGGTCTAA CCGACGACGA CTACGACATG TACTATGAGA TCTGGCAGCA ATTCGATCCG 
GAGGGCACCC AGTACATACG CTATGATCAG CTGTCCGAAT TCCTGGACGT ACTGGAGCCC 
CCGCTGCAGA TCCACAAACC GAACAAGTAC AAGATCATAT CGATGGACAT ACCCATCTGT 
CGCGGTGACC TCATGTACTG CGTCGACATC CTCGACGCCC TTACGAAAGA CTTCTTTGCG 
CGGAAGGGCA ATCCGATAGA GGAGACGGGT GAGATTGGTG AGATAGCGGC CCGCCCGGAT 
ACGGAGGGCT ACGAGCCCGT CTCATCAACG CTGTGGCGTC AGCGTGAGGA GTACTGCGCC 
CGGCTAATCC AGCACGCCTG GCGAAAGCAC AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT 
GAGCCGGATA CGGATCATGG CGATGGCGGT GATCCGGATG CCGGGGACCC GGCGCCCGAT 
GAAGCAACGG ACGGCGATGC GCCCGCTGGT GGAGATGGTA GTGTTAACGG TACTGCAGAA 
GGAGCTGCCG ATGCCGATGA GAGTAATGTA AATAGTCCGG GTGAGGATGC AGCGGCGGCG 
GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG GGCACGACGA CGGCGGGAAG TCCCGGAGCG 
GGTAGCGCCG GGCGACAGAC CGCCGTTCTC GTGGAGAGCG ACGGGTTCGT GACGAAGAAC 
GGCCACAAGG TGGTCATCCA CTCGCGATCG CCGAGCATCA CGTCGCGCAC GGCGGATGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188608.1
CDS	269..6571
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201679.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant M, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201679.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTATTCAAA CTGGCCAAGT CTTGGCCCAC ACTTAATTTA 
CTCATTTCGA TTATGGGACG CACCATGGGC GCTTTGGGTA ATCTGACATT TGTACTTTGC 
ATTATCATCT TCATCTTTGC GGTGATGGGA ATGCAACTGT TCGGAAAGAA TTATCATGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GAGCATGGTG ACAACGAACT GGAGCTGGGC 
CACGACGAGA TCCTCGCCGA CGGCCTCATC AAGAAGGGGA TCAAGGAGCA GACGCAACTG 
GAGGTGGCCA TCGGGGATGG CATGGAATTC ACGATACACG GCGACATGAA GAACAACAAG 
CCGAAGAAAT CCAAATATCT AAATAACGCA ACGGACGACG ACACTGCCAG CATTAACTCA 
TATGGTAGCC ATAAGAATCG ACCATTCAAG GACGAGAGCC ACAAGGGCAG CGCCGAGACG 
ATGGAGGGCG AGGAGAAGCG CGACGCCAGC AAGGAGGATT TAGGTCTCGA CGAGGAACTG 
GACGAGGAGG GCGAATGCGA GGAGGGCCCG CTCGACGGTG ATATCATTAT TCATGCACAC 
GACGAGGATA TACTCGATGA ATATCCAGCT GATTGCTGCC CCGATTCGTA CTATAAGAAA 
TTTCCGATCT TAGCCGGTGA CGATGACTCG CCGTTCTGGC AAGGATGGGG CAATTTACGA 
CTGAAAACTT TTCAATTAAT TGAAAATAAA TATTTTGAAA CAGCTGTTAT CACTATGATT 
TTAATGAGTA GCTTAGCTTT GGCATTAGAA GATGTACATC TGCCACAAAG ACCCATACTG 
CAGGATATTT TATACTATAT GGACAGAATA TTTACGGTTA TATTCTTCTT GGAAATGTTA 
ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG TACTTCACCA ACGCGTGGTG TTGGCTCGAT 
TTCGTGATTG TCATGGTATC GCTTATCAAC TTCGTTGCTT CACTTGTTGG AGCTGGTGGT 
ATTCAAGCCT TCAAGACTAT GCGAACGTTA AGAGCACTGA GACCACTACG TGCCATGTCC 
CGTATGCAGG GCATGAGGGT CGTCGTTAAT GCGCTGGTAC AAGCTATACC GTCCATCTTC 
AATGTGCTAT TGGTGTGTCT AATATTTTGG CTAATTTTTG CCATAATGGG TGTACAGCTT 
TTTGCTGGAA AATATTTTAA GTGCGAGGAC ATGAATGGCA CGAAGCTCAG CCACGAGATC 
ATACCAAATC GCAATGCCTG CGAGAGCGAG AACTACACGT GGGTGAATTC AGCAATGAAT 
TTCGATCATG TAGGTAACGC GTATCTGTGC CTTTTCCAAG TGGCCACCTT CAAAGGCTGG 
ATACAAATCA TGAACGATGC TATCGATTCA CGAGAGGTGG ACAAGCAACC AATTCGTGAA 
ACGAACATCT ACATGTATTT ATATTTCGTA TTCTTCATCA TATTTGGATC ATTTTTCACA 
CTCAATCTGT TCATTGGTGT TATCATTGAT AATTTTAATG AGCAAAAGAA AAAAGCAGGT 
GGATCATTAG AAATGTTCAT GACAGAAGAT CAGAAAAAGT ACTATAATGC TATGAAAAAG 
ATGGGCTCTA AAAAACCATT AAAAGCCATT CCAAGACCAA GGTGGCGACC ACAAGCAATA 
GTCTTTGAAA TAGTAACCGA TAAGAAATTC GATATAATCA TTATGTTATT CATTGGTCTG 
AACATGTTCA CCATGACCCT CGATCGTTAC GATGCGTCGG ACACGTATAA CGCGGTCCTA 
GACTATCTCA ATGCGATATT CGTAGTTATT TTCAGTTCCG AATGTCTATT AAAAATATTC 
GCTTTACGAT ATCACTATTT TATTGAGCCA TGGAATTTAT TTGATGTAGT AGTTGTCATT 
TTATCCATCT TAGGTCTTGT ACTTAGCGAT ATTATCGAGA AGTACTTCGT GTCGCCGACC 
CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG GGCCGTGTCC TTCGACTGGT GAAGGGAGCC 
AAGGGCATTC GGACACTGCT CTTCGCGTTG GCCATGTCGC TGCCGGCCCT GTTCAACATC 
TGCCTGCTGC TGTTCCTGGT CATGTTCATC TTTGCCATTT TCGGCATGTC GTTCTTCATG 
CACGTGAAGG AGAAGAGCGG CATCAACGAC GTCTACAACT TCAAGACCTT TGGCCAGAGC 
ATGATCCTGC TCTTTCAGAT GTCGACGTCA GCCGGTTGGG ATGGTGTACT GGACGCCATT 
ATCAATGAGG AAGCATGCGA TCCACCCGAC AACGACAAAG GCTATCCGGG CAATTGTGGT 
TCAGCGACCG TTGGAATAAC GTTTCTCCTC TCATACCTAG TTATAAGCTT TTTGATAGTT 
ATTAATATGT ACATTGCTGT CATTCTCGAG AACTATAGTC AGGCCACCGA GGACGTGCAA 
GAGGGTCTAA CCGACGACGA CTACGACATG TACTATGAGA TCTGGCAGCA ATTCGATCCG 
GAGGGCACCC AGTACATACG CTATGATCAG CTGTCCGAAT TCCTGGACGT ACTGGAGCCC 
CCGCTGCAGA TCCACAAACC GAACAAGTAC AAGATCATAT CGATGGACAT ACCCATCTGT 
CGCGGTGACC TCATGTACTG CGTCGACATC CTCGACGCCC TTACGAAAGA CTTCTTTGCG 
CGGAAGGGCA ATCCGATAGA GGAGACGGGT GAGATTGGTG AGATAGCGGC CCGCCCGGAT 
ACGGAGGGCT ACGAGCCCGT CTCATCAACG CTGTGGCGTC AGCGTGAGGA GTACTGCGCC 
CGGCTAATCC AGCACGCCTG GCGAAAGCAC AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT 
GAGCCGGATA CGGATCATGG CGATGGCGGT GATCCGGATG CCGGGGACCC GGCGCCCGAT 
GAAGCAACGG ACGGCGATGC GCCCGCTGGT GGAGATGGTA GTGTTAACGG TACTGCAGAA 
GGAGCTGCCG ATGCCGATGA GAGTAATGTA AATAGTCCGG GTGAGGATGC AGCGGCGGCG 
GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG GGCACGACGA CGGCGGGAAG TCCCGGAGCG 
GGTAGCGCCG GGCGACAGAC CGCCGTTCTC GTGGAGAGCG ACGGGTTCGT GACGAAGAAC 
GGCCACAAGG TGGTCATCCA CTCGCGATCG CCGAGCATCA CGTCGCGCAC GGCGGATGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201686.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188615.1 Drosophila melanogaster paralytic (para), transcript variant T, mRNA.

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CloneID	OFa01815
Clone ID Related Accession (Same CDS sequence)	NM_001201686.1
Accession Version	NM_001201686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform T
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTATTCAAA CTGGCCAAGT CTTGGCCCAC ACTTAATTTA 
CTCATTTCGA TTATGGGACG CACCATGGGC GCTTTGGGTA ATCTGACATT TGTACTTTGC 
ATTATCATCT TCATCTTTGC GGTGATGGGA ATGCAACTGT TCGGAAAGAA TTATCATGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GGCAAAGGCG TTTGTCGTTG TATATCTGCA 
GAGCATGGTG ACAACGAACT GGAGCTGGGC CACGACGAGA TCCTCGCCGA CGGCCTCATC 
AAGAAGGGGA TCAAGGAGCA GACGCAACTG GAGGTGGCCA TCGGGGATGG CATGGAATTC 
ACGATACACG GCGACATGAA GAACAACAAG CCGAAGAAAT CCAAATATCT AAATAACGCA 
ACGGACGACG ACACTGCCAG CATTAACTCA TATGGTAGCC ATAAGAATCG ACCATTCAAG 
GACGAGAGCC ACAAGGGCAG CGCCGAGACG ATGGAGGGCG AGGAGAAGCG CGACGCCAGC 
AAGGAGGATT TAGGTCTCGA CGAGGAACTG GACGAGGAGG GCGAATGCGA GGAGGGCCCG 
CTCGACGGTG ATATCATTAT TCATGCACAC GACGAGGATA TACTCGATGA ATATCCAGCT 
GATTGCTGCC CCGATTCGTA CTATAAGAAA TTTCCGATCT TAGCCGGTGA CGATGACTCG 
CCGTTCTGGC AAGGATGGGG CAATTTACGA CTGAAAACTT TTCAATTAAT TGAAAATAAA 
TATTTTGAAA CAGCTGTTAT CACTATGATT TTAATGAGTA GCTTAGCTTT GGCATTAGAA 
GATGTACATC TGCCACAAAG ACCCATACTG CAGGATATTT TATACTATAT GGACAGAATA 
TTTACGGTTA TATTCTTCTT GGAAATGTTA ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG 
TACTTCACCA ACGCGTGGTG TTGGCTCGAT TTCGTGATTG TCATGGTATC GCTTATCAAC 
TTCGTTGCTT CACTTGTTGG AGCTGGTGGT ATTCAAGCCT TCAAGACTAT GCGAACGTTA 
AGAGCACTGA GACCACTACG TGCCATGTCC CGTATGCAGG GCATGAGGGT CGTCGTTAAT 
GCGCTGGTAC AAGCTATACC GTCCATCTTC AATGTGCTAT TGGTGTGTCT AATATTTTGG 
CTAATTTTTG CCATAATGGG TGTACAGCTT TTTGCTGGAA AATATTTTAA GTGCGAGGAC 
ATGAATGGCA CGAAGCTCAG CCACGAGATC ATACCAAATC GCAATGCCTG CGAGAGCGAG 
AACTACACGT GGGTGAATTC AGCAATGAAT TTCGATCATG TAGGTAACGC GTATCTGTGC 
CTTTTCCAAG TGGCCACCTT CAAAGGCTGG ATACAAATCA TGAACGATGC TATCGATTCA 
CGAGAGGTGG ACAAGCAACC AATTCGTGAA ACGAACATCT ACATGTATTT ATATTTCGTA 
TTCTTCATCA TATTTGGATC ATTTTTCACA CTCAATCTGT TCATTGGTGT TATCATTGAT 
AATTTTAATG AGCAAAAGAA AAAAGCAGGT GGATCATTAG AAATGTTCAT GACAGAAGAT 
CAGAAAAAGT ACTATAATGC TATGAAAAAG ATGGGCTCTA AAAAACCATT AAAAGCCATT 
CCAAGACCAA GGTGGCGACC ACAAGCAATA GTCTTTGAAA TAGTAACCGA TAAGAAATTC 
GATATAATCA TTATGTTATT CATTGGTCTG AACATGTTCA CCATGACCCT CGATCGTTAC 
GATGCGTCGG ACACGTATAA CGCGGTCCTA GACTATCTCA ATGCGATATT CGTAGTTATT 
TTCAGTTCCG AATGTCTATT AAAAATATTC GCTTTACGAT ATCACTATTT TATTGAGCCA 
TGGAATTTAT TTGATGTAGT AGTTGTCATT TTATCCATCT TAGGTCTTGT ACTTAGCGAT 
ATTATCGAGA AGTACTTCGT GTCGCCGACC CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG 
GGCCGTGTCC TTCGACTGGT GAAGGGAGCC AAGGGCATTC GGACACTGCT CTTCGCGTTG 
GCCATGTCGC TGCCGGCCCT GTTCAACATC TGCCTGCTGC TGTTCCTGGT CATGTTCATC 
TTTGCCATTT TCGGCATGTC GTTCTTCATG CACGTGAAGG AGAAGAGCGG CATCAACGAC 
GTCTACAACT TCAAGACCTT TGGCCAGAGC ATGATCCTGC TCTTTCAGAT GTCGACGTCA 
GCCGGTTGGG ATGGTGTACT GGACGCCATT ATCAATGAGG AAGCATGCGA TCCACCCGAC 
AACGACAAAG GCTATCCGGG CAATTGTGGT TCAGCGACCG TTGGAATAAC GTTTCTCCTC 
TCATACCTAG TTATAAGCTT TTTGATAGTT ATTAATATGT ACATTGCTGT CATTCTCGAG 
AACTATAGTC AGGCCACCGA GGACGTGCAA GAGGGTCTAA CCGACGACGA CTACGACATG 
TACTATGAGA TCTGGCAGCA ATTCGATCCG GAGGGCACCC AGTACATACG CTATGATCAG 
CTGTCCGAAT TCCTGGACGT ACTGGAGCCC CCGCTGCAGA TCCACAAACC GAACAAGTAC 
AAGATCATAT CGATGGACAT ACCCATCTGT CGCGGTGACC TCATGTACTG CGTCGACATC 
CTCGACGCCC TTACGAAAGA CTTCTTTGCG CGGAAGGGCA ATCCGATAGA GGAGACGGGT 
GAGATTGGTG AGATAGCGGC CCGCCCGGAT ACGGAGGGCT ACGAGCCCGT CTCATCAACG 
CTGTGGCGTC AGCGTGAGGA GTACTGCGCC CGGCTAATCC AGCACGCCTG GCGAAAGCAC 
AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT GAGCCGGATA CGGATCATGG CGATGGCGGT 
GATCCGGATG CCGGGGACCC GGCGCCCGAT GAAGCAACGG ACGGCGATGC GCCCGCTGGT 
GGAGATGGTA GTGTTAACGG TACTGCAGAA GGAGCTGCCG ATGCCGATGA GAGTAATGTA 
AATAGTCCGG GTGAGGATGC AGCGGCGGCG GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG 
GGCACGACGA CGGCGGGAAG TCCCGGAGCG GGTAGCGCCG GGCGACAGAC CGCCGTTCTC 
GTGGAGAGCG ACGGGTTCGT GACGAAGAAC GGCCACAAGG TGGTCATCCA CTCGCGATCG 
CCGAGCATCA CGTCGCGCAC GGCGGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188615.1
CDS	269..6601
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201686.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant T, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201686.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTATTCAAA CTGGCCAAGT CTTGGCCCAC ACTTAATTTA 
CTCATTTCGA TTATGGGACG CACCATGGGC GCTTTGGGTA ATCTGACATT TGTACTTTGC 
ATTATCATCT TCATCTTTGC GGTGATGGGA ATGCAACTGT TCGGAAAGAA TTATCATGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GGCAAAGGCG TTTGTCGTTG TATATCTGCA 
GAGCATGGTG ACAACGAACT GGAGCTGGGC CACGACGAGA TCCTCGCCGA CGGCCTCATC 
AAGAAGGGGA TCAAGGAGCA GACGCAACTG GAGGTGGCCA TCGGGGATGG CATGGAATTC 
ACGATACACG GCGACATGAA GAACAACAAG CCGAAGAAAT CCAAATATCT AAATAACGCA 
ACGGACGACG ACACTGCCAG CATTAACTCA TATGGTAGCC ATAAGAATCG ACCATTCAAG 
GACGAGAGCC ACAAGGGCAG CGCCGAGACG ATGGAGGGCG AGGAGAAGCG CGACGCCAGC 
AAGGAGGATT TAGGTCTCGA CGAGGAACTG GACGAGGAGG GCGAATGCGA GGAGGGCCCG 
CTCGACGGTG ATATCATTAT TCATGCACAC GACGAGGATA TACTCGATGA ATATCCAGCT 
GATTGCTGCC CCGATTCGTA CTATAAGAAA TTTCCGATCT TAGCCGGTGA CGATGACTCG 
CCGTTCTGGC AAGGATGGGG CAATTTACGA CTGAAAACTT TTCAATTAAT TGAAAATAAA 
TATTTTGAAA CAGCTGTTAT CACTATGATT TTAATGAGTA GCTTAGCTTT GGCATTAGAA 
GATGTACATC TGCCACAAAG ACCCATACTG CAGGATATTT TATACTATAT GGACAGAATA 
TTTACGGTTA TATTCTTCTT GGAAATGTTA ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG 
TACTTCACCA ACGCGTGGTG TTGGCTCGAT TTCGTGATTG TCATGGTATC GCTTATCAAC 
TTCGTTGCTT CACTTGTTGG AGCTGGTGGT ATTCAAGCCT TCAAGACTAT GCGAACGTTA 
AGAGCACTGA GACCACTACG TGCCATGTCC CGTATGCAGG GCATGAGGGT CGTCGTTAAT 
GCGCTGGTAC AAGCTATACC GTCCATCTTC AATGTGCTAT TGGTGTGTCT AATATTTTGG 
CTAATTTTTG CCATAATGGG TGTACAGCTT TTTGCTGGAA AATATTTTAA GTGCGAGGAC 
ATGAATGGCA CGAAGCTCAG CCACGAGATC ATACCAAATC GCAATGCCTG CGAGAGCGAG 
AACTACACGT GGGTGAATTC AGCAATGAAT TTCGATCATG TAGGTAACGC GTATCTGTGC 
CTTTTCCAAG TGGCCACCTT CAAAGGCTGG ATACAAATCA TGAACGATGC TATCGATTCA 
CGAGAGGTGG ACAAGCAACC AATTCGTGAA ACGAACATCT ACATGTATTT ATATTTCGTA 
TTCTTCATCA TATTTGGATC ATTTTTCACA CTCAATCTGT TCATTGGTGT TATCATTGAT 
AATTTTAATG AGCAAAAGAA AAAAGCAGGT GGATCATTAG AAATGTTCAT GACAGAAGAT 
CAGAAAAAGT ACTATAATGC TATGAAAAAG ATGGGCTCTA AAAAACCATT AAAAGCCATT 
CCAAGACCAA GGTGGCGACC ACAAGCAATA GTCTTTGAAA TAGTAACCGA TAAGAAATTC 
GATATAATCA TTATGTTATT CATTGGTCTG AACATGTTCA CCATGACCCT CGATCGTTAC 
GATGCGTCGG ACACGTATAA CGCGGTCCTA GACTATCTCA ATGCGATATT CGTAGTTATT 
TTCAGTTCCG AATGTCTATT AAAAATATTC GCTTTACGAT ATCACTATTT TATTGAGCCA 
TGGAATTTAT TTGATGTAGT AGTTGTCATT TTATCCATCT TAGGTCTTGT ACTTAGCGAT 
ATTATCGAGA AGTACTTCGT GTCGCCGACC CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG 
GGCCGTGTCC TTCGACTGGT GAAGGGAGCC AAGGGCATTC GGACACTGCT CTTCGCGTTG 
GCCATGTCGC TGCCGGCCCT GTTCAACATC TGCCTGCTGC TGTTCCTGGT CATGTTCATC 
TTTGCCATTT TCGGCATGTC GTTCTTCATG CACGTGAAGG AGAAGAGCGG CATCAACGAC 
GTCTACAACT TCAAGACCTT TGGCCAGAGC ATGATCCTGC TCTTTCAGAT GTCGACGTCA 
GCCGGTTGGG ATGGTGTACT GGACGCCATT ATCAATGAGG AAGCATGCGA TCCACCCGAC 
AACGACAAAG GCTATCCGGG CAATTGTGGT TCAGCGACCG TTGGAATAAC GTTTCTCCTC 
TCATACCTAG TTATAAGCTT TTTGATAGTT ATTAATATGT ACATTGCTGT CATTCTCGAG 
AACTATAGTC AGGCCACCGA GGACGTGCAA GAGGGTCTAA CCGACGACGA CTACGACATG 
TACTATGAGA TCTGGCAGCA ATTCGATCCG GAGGGCACCC AGTACATACG CTATGATCAG 
CTGTCCGAAT TCCTGGACGT ACTGGAGCCC CCGCTGCAGA TCCACAAACC GAACAAGTAC 
AAGATCATAT CGATGGACAT ACCCATCTGT CGCGGTGACC TCATGTACTG CGTCGACATC 
CTCGACGCCC TTACGAAAGA CTTCTTTGCG CGGAAGGGCA ATCCGATAGA GGAGACGGGT 
GAGATTGGTG AGATAGCGGC CCGCCCGGAT ACGGAGGGCT ACGAGCCCGT CTCATCAACG 
CTGTGGCGTC AGCGTGAGGA GTACTGCGCC CGGCTAATCC AGCACGCCTG GCGAAAGCAC 
AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT GAGCCGGATA CGGATCATGG CGATGGCGGT 
GATCCGGATG CCGGGGACCC GGCGCCCGAT GAAGCAACGG ACGGCGATGC GCCCGCTGGT 
GGAGATGGTA GTGTTAACGG TACTGCAGAA GGAGCTGCCG ATGCCGATGA GAGTAATGTA 
AATAGTCCGG GTGAGGATGC AGCGGCGGCG GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG 
GGCACGACGA CGGCGGGAAG TCCCGGAGCG GGTAGCGCCG GGCGACAGAC CGCCGTTCTC 
GTGGAGAGCG ACGGGTTCGT GACGAAGAAC GGCCACAAGG TGGTCATCCA CTCGCGATCG 
CCGAGCATCA CGTCGCGCAC GGCGGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201707.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188636.1 Drosophila melanogaster paralytic (para), transcript variant AO, mRNA.

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CloneID	OFa01816
Clone ID Related Accession (Same CDS sequence)	NM_001201707.1
Accession Version	NM_001201707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AO
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTATTCAAA CTGGCCAAGT CTTGGCCCAC ACTTAATTTA 
CTCATTTCGA TTATGGGACG CACCATGGGC GCTTTGGGTA ATCTGACATT TGTACTTTGC 
ATTATCATCT TCATCTTTGC GGTGATGGGA ATGCAACTGT TCGGAAAGAA TTATCATGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GGTGAGAGGA CCAACCAGAT CAGTTGGATT 
TGGAGCGAAG AGCATGGTGA CAACGAACTG GAGCTGGGCC ACGACGAGAT CCTCGCCGAC 
GGCCTCATCA AGAAGGGGAT CAAGGAGCAG ACGCAACTGG AGGTGGCCAT CGGGGATGGC 
ATGGAATTCA CGATACACGG CGACATGAAG AACAACAAGC CGAAGAAATC CAAATATCTA 
AATAACGCAA CGGACGACGA CACTGCCAGC ATTAACTCAT ATGGTAGCCA TAAGAATCGA 
CCATTCAAGG ACGAGAGCCA CAAGGGCAGC GCCGAGACGA TGGAGGGCGA GGAGAAGCGC 
GACGCCAGCA AGGAGGATTT AGGTCTCGAC GAGGAACTGG ACGAGGAGGG CGAATGCGAG 
GAGGGCCCGC TCGACGGTGA TATCATTATT CATGCACACG ACGAGGATAT ACTCGATGAA 
TATCCAGCTG ATTGCTGCCC CGATTCGTAC TATAAGAAAT TTCCGATCTT AGCCGGTGAC 
GATGACTCGC CGTTCTGGCA AGGATGGGGC AATTTACGAC TGAAAACTTT TCAATTAATT 
GAAAATAAAT ATTTTGAAAC AGCTGTTATC ACTATGATTT TAATGAGTAG CTTAGCTTTG 
GCATTAGAAG ATGTACATCT GCCACAAAGA CCCATACTGC AGGATATTTT ATACTATATG 
GACAGAATAT TTACGGTTAT ATTCTTCTTG GAAATGTTAA TCAAGTGGTT GGCGCTCGGC 
TTCAAAGTGT ACTTCACCAA CGCGTGGTGT TGGCTCGATT TCGTGATTGT CATGGTATCG 
CTTATCAACT TCGTTGCTTC ACTTGTTGGA GCTGGTGGTA TTCAAGCCTT CAAGACTATG 
CGAACGTTAA GAGCACTGAG ACCACTACGT GCCATGTCCC GTATGCAGGG CATGAGGGTC 
GTCGTTAATG CGCTGGTACA AGCTATACCG TCCATCTTCA ATGTGCTATT GGTGTGTCTA 
ATATTTTGGC TAATTTTTGC CATAATGGGT GTACAGCTTT TTGCTGGAAA ATATTTTAAG 
TGCGAGGACA TGAATGGCAC GAAGCTCAGC CACGAGATCA TACCAAATCG CAATGCCTGC 
GAGAGCGAGA ACTACACGTG GGTGAATTCA GCAATGAATT TCGATCATGT AGGTAACGCG 
TATCTGTGCC TTTTCCAAGT GGCCACCTTC AAAGGCTGGA TACAAATCAT GAACGATGCT 
ATCGATTCAC GAGAGGTGGA CAAGCAACCA ATTCGTGAAA CGAACATCTA CATGTATTTA 
TATTTCGTAT TCTTCATCAT ATTTGGATCA TTTTTCACAC TCAATCTGTT CATTGGTGTT 
ATCATTGATA ATTTTAATGA GCAAAAGAAA AAAGCAGGTG GATCATTAGA AATGTTCATG 
ACAGAAGATC AGAAAAAGTA CTATAATGCT ATGAAAAAGA TGGGCTCTAA AAAACCATTA 
AAAGCCATTC CAAGACCAAG GTGGCGACCA CAAGCAATAG TCTTTGAAAT AGTAACCGAT 
AAGAAATTCG ATATAATCAT TATGTTATTC ATTGGTCTGA ACATGTTCAC CATGACCCTC 
GATCGTTACG ATGCGTCGGA CACGTATAAC GCGGTCCTAG ACTATCTCAA TGCGATATTC 
GTAGTTATTT TCAGTTCCGA ATGTCTATTA AAAATATTCG CTTTACGATA TCACTATTTT 
ATTGAGCCAT GGAATTTATT TGATGTAGTA GTTGTCATTT TATCCATCTT AGGTCTTGTA 
CTTAGCGATA TTATCGAGAA GTACTTCGTG TCGCCGACCC TGCTCCGAGT GGTGCGTGTG 
GCGAAAGTGG GCCGTGTCCT TCGACTGGTG AAGGGAGCCA AGGGCATTCG GACACTGCTC 
TTCGCGTTGG CCATGTCGCT GCCGGCCCTG TTCAACATCT GCCTGCTGCT GTTCCTGGTC 
ATGTTCATCT TTGCCATTTT CGGCATGTCG TTCTTCATGC ACGTGAAGGA GAAGAGCGGC 
ATCAACGACG TCTACAACTT CAAGACCTTT GGCCAGAGCA TGATCCTGCT CTTTCAGATG 
TCGACGTCAG CCGGTTGGGA TGGTGTACTG GACGCCATTA TCAATGAGGA AGCATGCGAT 
CCACCCGACA ACGACAAAGG CTATCCGGGC AATTGTGGTT CAGCGACCGT TGGAATAACG 
TTTCTCCTCT CATACCTAGT TATAAGCTTT TTGATAGTTA TTAATATGTA CATTGCTGTC 
ATTCTCGAGA ACTATAGTCA GGCCACCGAG GACGTGCAAG AGGGTCTAAC CGACGACGAC 
TACGACATGT ACTATGAGAT CTGGCAGCAA TTCGATCCGG AGGGCACCCA GTACATACGC 
TATGATCAGC TGTCCGAATT CCTGGACGTA CTGGAGCCCC CGCTGCAGAT CCACAAACCG 
AACAAGTACA AGATCATATC GATGGACATA CCCATCTGTC GCGGTGACCT CATGTACTGC 
GTCGACATCC TCGACGCCCT TACGAAAGAC TTCTTTGCGC GGAAGGGCAA TCCGATAGAG 
GAGACGGGTG AGATTGGTGA GATAGCGGCC CGCCCGGATA CGGAGGGCTA CGAGCCCGTC 
TCATCAACGC TGTGGCGTCA GCGTGAGGAG TACTGCGCCC GGCTAATCCA GCACGCCTGG 
CGAAAGCACA AGGCGCGCGG CGAGGGAGGT GGGTCCTTTG AGCCGGATAC GGATCATGGC 
GATGGCGGTG ATCCGGATGC CGGGGACCCG GCGCCCGATG AAGCAACGGA CGGCGATGCG 
CCCGCTGGTG GAGATGGTAG TGTTAACGGT ACTGCAGAAG GAGCTGCCGA TGCCGATGAG 
AGTAATGTAA ATAGTCCGGG TGAGGATGCA GCGGCGGCGG CAGCAGCAGC AGCAGCAGCG 
GCGGCGGCGG GCACGACGAC GGCGGGAAGT CCCGGAGCGG GTAGCGCCGG GCGACAGACC 
GCCGTTCTCG TGGAGAGCGA CGGGTTCGTG ACGAAGAACG GCCACAAGGT GGTCATCCAC 
TCGCGATCGC CGAGCATCAC GTCGCGCACG GCGGATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188636.1
CDS	269..6610
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201707.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AO, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201707.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTATTCAAA CTGGCCAAGT CTTGGCCCAC ACTTAATTTA 
CTCATTTCGA TTATGGGACG CACCATGGGC GCTTTGGGTA ATCTGACATT TGTACTTTGC 
ATTATCATCT TCATCTTTGC GGTGATGGGA ATGCAACTGT TCGGAAAGAA TTATCATGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GGTGAGAGGA CCAACCAGAT CAGTTGGATT 
TGGAGCGAAG AGCATGGTGA CAACGAACTG GAGCTGGGCC ACGACGAGAT CCTCGCCGAC 
GGCCTCATCA AGAAGGGGAT CAAGGAGCAG ACGCAACTGG AGGTGGCCAT CGGGGATGGC 
ATGGAATTCA CGATACACGG CGACATGAAG AACAACAAGC CGAAGAAATC CAAATATCTA 
AATAACGCAA CGGACGACGA CACTGCCAGC ATTAACTCAT ATGGTAGCCA TAAGAATCGA 
CCATTCAAGG ACGAGAGCCA CAAGGGCAGC GCCGAGACGA TGGAGGGCGA GGAGAAGCGC 
GACGCCAGCA AGGAGGATTT AGGTCTCGAC GAGGAACTGG ACGAGGAGGG CGAATGCGAG 
GAGGGCCCGC TCGACGGTGA TATCATTATT CATGCACACG ACGAGGATAT ACTCGATGAA 
TATCCAGCTG ATTGCTGCCC CGATTCGTAC TATAAGAAAT TTCCGATCTT AGCCGGTGAC 
GATGACTCGC CGTTCTGGCA AGGATGGGGC AATTTACGAC TGAAAACTTT TCAATTAATT 
GAAAATAAAT ATTTTGAAAC AGCTGTTATC ACTATGATTT TAATGAGTAG CTTAGCTTTG 
GCATTAGAAG ATGTACATCT GCCACAAAGA CCCATACTGC AGGATATTTT ATACTATATG 
GACAGAATAT TTACGGTTAT ATTCTTCTTG GAAATGTTAA TCAAGTGGTT GGCGCTCGGC 
TTCAAAGTGT ACTTCACCAA CGCGTGGTGT TGGCTCGATT TCGTGATTGT CATGGTATCG 
CTTATCAACT TCGTTGCTTC ACTTGTTGGA GCTGGTGGTA TTCAAGCCTT CAAGACTATG 
CGAACGTTAA GAGCACTGAG ACCACTACGT GCCATGTCCC GTATGCAGGG CATGAGGGTC 
GTCGTTAATG CGCTGGTACA AGCTATACCG TCCATCTTCA ATGTGCTATT GGTGTGTCTA 
ATATTTTGGC TAATTTTTGC CATAATGGGT GTACAGCTTT TTGCTGGAAA ATATTTTAAG 
TGCGAGGACA TGAATGGCAC GAAGCTCAGC CACGAGATCA TACCAAATCG CAATGCCTGC 
GAGAGCGAGA ACTACACGTG GGTGAATTCA GCAATGAATT TCGATCATGT AGGTAACGCG 
TATCTGTGCC TTTTCCAAGT GGCCACCTTC AAAGGCTGGA TACAAATCAT GAACGATGCT 
ATCGATTCAC GAGAGGTGGA CAAGCAACCA ATTCGTGAAA CGAACATCTA CATGTATTTA 
TATTTCGTAT TCTTCATCAT ATTTGGATCA TTTTTCACAC TCAATCTGTT CATTGGTGTT 
ATCATTGATA ATTTTAATGA GCAAAAGAAA AAAGCAGGTG GATCATTAGA AATGTTCATG 
ACAGAAGATC AGAAAAAGTA CTATAATGCT ATGAAAAAGA TGGGCTCTAA AAAACCATTA 
AAAGCCATTC CAAGACCAAG GTGGCGACCA CAAGCAATAG TCTTTGAAAT AGTAACCGAT 
AAGAAATTCG ATATAATCAT TATGTTATTC ATTGGTCTGA ACATGTTCAC CATGACCCTC 
GATCGTTACG ATGCGTCGGA CACGTATAAC GCGGTCCTAG ACTATCTCAA TGCGATATTC 
GTAGTTATTT TCAGTTCCGA ATGTCTATTA AAAATATTCG CTTTACGATA TCACTATTTT 
ATTGAGCCAT GGAATTTATT TGATGTAGTA GTTGTCATTT TATCCATCTT AGGTCTTGTA 
CTTAGCGATA TTATCGAGAA GTACTTCGTG TCGCCGACCC TGCTCCGAGT GGTGCGTGTG 
GCGAAAGTGG GCCGTGTCCT TCGACTGGTG AAGGGAGCCA AGGGCATTCG GACACTGCTC 
TTCGCGTTGG CCATGTCGCT GCCGGCCCTG TTCAACATCT GCCTGCTGCT GTTCCTGGTC 
ATGTTCATCT TTGCCATTTT CGGCATGTCG TTCTTCATGC ACGTGAAGGA GAAGAGCGGC 
ATCAACGACG TCTACAACTT CAAGACCTTT GGCCAGAGCA TGATCCTGCT CTTTCAGATG 
TCGACGTCAG CCGGTTGGGA TGGTGTACTG GACGCCATTA TCAATGAGGA AGCATGCGAT 
CCACCCGACA ACGACAAAGG CTATCCGGGC AATTGTGGTT CAGCGACCGT TGGAATAACG 
TTTCTCCTCT CATACCTAGT TATAAGCTTT TTGATAGTTA TTAATATGTA CATTGCTGTC 
ATTCTCGAGA ACTATAGTCA GGCCACCGAG GACGTGCAAG AGGGTCTAAC CGACGACGAC 
TACGACATGT ACTATGAGAT CTGGCAGCAA TTCGATCCGG AGGGCACCCA GTACATACGC 
TATGATCAGC TGTCCGAATT CCTGGACGTA CTGGAGCCCC CGCTGCAGAT CCACAAACCG 
AACAAGTACA AGATCATATC GATGGACATA CCCATCTGTC GCGGTGACCT CATGTACTGC 
GTCGACATCC TCGACGCCCT TACGAAAGAC TTCTTTGCGC GGAAGGGCAA TCCGATAGAG 
GAGACGGGTG AGATTGGTGA GATAGCGGCC CGCCCGGATA CGGAGGGCTA CGAGCCCGTC 
TCATCAACGC TGTGGCGTCA GCGTGAGGAG TACTGCGCCC GGCTAATCCA GCACGCCTGG 
CGAAAGCACA AGGCGCGCGG CGAGGGAGGT GGGTCCTTTG AGCCGGATAC GGATCATGGC 
GATGGCGGTG ATCCGGATGC CGGGGACCCG GCGCCCGATG AAGCAACGGA CGGCGATGCG 
CCCGCTGGTG GAGATGGTAG TGTTAACGGT ACTGCAGAAG GAGCTGCCGA TGCCGATGAG 
AGTAATGTAA ATAGTCCGGG TGAGGATGCA GCGGCGGCGG CAGCAGCAGC AGCAGCAGCG 
GCGGCGGCGG GCACGACGAC GGCGGGAAGT CCCGGAGCGG GTAGCGCCGG GCGACAGACC 
GCCGTTCTCG TGGAGAGCGA CGGGTTCGTG ACGAAGAACG GCCACAAGGT GGTCATCCAC 
TCGCGATCGC CGAGCATCAC GTCGCGCACG GCGGATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201676.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188605.1 Drosophila melanogaster paralytic (para), transcript variant J, mRNA.

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CloneID	OFa01817
Clone ID Related Accession (Same CDS sequence)	NM_001201676.1
Accession Version	NM_001201676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTTTTCAAG CTGGCCAAAT CGTGGCCGAC GCTGAATTTG 
CTCATATCGA TTATGGGCCG CACTGTCGGC GCCCTCGGCA ATCTGACCTT CGTCCTGTGC 
ATTATTATCT TCATATTCGC GGTCATGGGC ATGCAGCTGT TTGGCAAGAA CTACACAGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GAGCATGGTG ACAACGAACT GGAGCTGGGC 
CACGACGAGA TCCTCGCCGA CGGCCTCATC AAGAAGGGGA TCAAGGAGCA GACGCAACTG 
GAGGTGGCCA TCGGGGATGG CATGGAATTC ACGATACACG GCGACATGAA GAACAACAAG 
CCGAAGAAAT CCAAATATCT AAATAACGCA ACGGACGACG ACACTGCCAG CATTAACTCA 
TATGGTAGCC ATAAGAATCG ACCATTCAAG GACGAGAGCC ACAAGGGCAG CGCCGAGACG 
ATGGAGGGCG AGGAGAAGCG CGACGCCAGC AAGGAGGATT TAGGTCTCGA CGAGGAACTG 
GACGAGGAGG GCGAATGCGA GGAGGGCCCG CTCGACGGTG ATATCATTAT TCATGCACAC 
GACGAGGATA TACTCGATGA ATATCCAGCT GATTGCTGCC CCGATTCGTA CTATAAGAAA 
TTTCCGATCT TAGCCGGTGA CGATGACTCG CCGTTCTGGC AAGGATGGGG CAATTTACGA 
CTGAAAACTT TTCAATTAAT TGAAAATAAA TATTTTGAAA CAGCTGTTAT CACTATGATT 
TTAATGAGTA GCTTAGCTTT GGCATTAGAA GATGTACATC TGCCACAAAG ACCCATACTG 
CAGGATATTT TATACTATAT GGACAGAATA TTTACGGTTA TATTCTTCTT GGAAATGTTA 
ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG TACTTCACCA ACGCGTGGTG TTGGCTCGAT 
TTCGTGATTG TCATGGTATC GCTTATCAAC TTCGTTGCTT CACTTGTTGG AGCTGGTGGT 
ATTCAAGCCT TCAAGACTAT GCGAACGTTA AGAGCACTGA GACCACTACG TGCCATGTCC 
CGTATGCAGG GCATGAGGGT CGTCGTTAAT GCGCTGGTAC AAGCTATACC GTCCATCTTC 
AATGTGCTAT TGGTGTGTCT AATATTTTGG CTAATTTTTG CCATAATGGG TGTACAGCTT 
TTTGCTGGAA AATATTTTAA GTGCGAGGAC ATGAATGGCA CGAAGCTCAG CCACGAGATC 
ATACCAAATC GCAATGCCTG CGAGAGCGAG AACTACACGT GGGTGAATTC AGCAATGAAT 
TTCGATCATG TAGGTAACGC GTATCTGTGC CTTTTCCAAG TGGCCACCTT CAAAGGCTGG 
ATACAAATCA TGAACGATGC TATCGATTCA CGAGAGGTGG ACAAGCAACC AATTCGTGAA 
ACGAACATCT ACATGTATTT ATATTTCGTA TTCTTCATCA TATTTGGATC ATTTTTCACA 
CTCAATCTGT TCATTGGTGT TATCATTGAT AATTTTAATG AGCAAAAGAA AAAAGCAGGT 
GGATCATTAG AAATGTTCAT GACAGAAGAT CAGAAAAAGT ACTATAATGC TATGAAAAAG 
ATGGGCTCTA AAAAACCATT AAAAGCCATT CCAAGACCAA GGTGGCGACC ACAAGCAATA 
GTCTTTGAAA TAGTAACCGA TAAGAAATTC GATATAATCA TTATGTTATT CATTGGTCTG 
AACATGTTCA CCATGACCCT CGATCGTTAC GATGCGTCGG ACACGTATAA CGCGGTCCTA 
GACTATCTCA ATGCGATATT CGTAGTTATT TTCAGTTCCG AATGTCTATT AAAAATATTC 
GCTTTACGAT ATCACTATTT TATTGAGCCA TGGAATTTAT TTGATGTAGT AGTTGTCATT 
TTATCCATCT TAGGTCTTGT ACTTAGCGAT ATTATCGAGA AGTACTTCGT GTCGCCGACC 
CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG GGCCGTGTCC TTCGACTGGT GAAGGGAGCC 
AAGGGCATTC GGACACTGCT CTTCGCGTTG GCCATGTCGC TGCCGGCCCT GTTCAACATC 
TGCCTGCTGC TGTTCCTGGT CATGTTCATC TTTGCCATTT TCGGCATGTC GTTCTTCATG 
CACGTGAAGG AGAAGAGCGG CATCAACGAC GTCTACAACT TCAAGACCTT TGGCCAGAGC 
ATGATCCTGC TCTTTCAGAT GTCGACGTCA GCCGGTTGGG ATGGTGTACT GGACGCCATT 
ATCAATGAGG AAGCATGCGA TCCACCCGAC AACGACAAAG GCTATCCGGG CAATTGTGGT 
TCAGCGACCG TTGGAATAAC GTTTCTCCTC TCATACCTAG TTATAAGCTT TTTGATAGTT 
ATTAATATGT ACATTGCTGT CATTCTCGAG AACTATAGTC AGGCCACCGA GGACGTGCAA 
GAGGGTCTAA CCGACGACGA CTACGACATG TACTATGAGA TCTGGCAGCA ATTCGATCCG 
GAGGGCACCC AGTACATACG CTATGATCAG CTGTCCGAAT TCCTGGACGT ACTGGAGCCC 
CCGCTGCAGA TCCACAAACC GAACAAGTAC AAGATCATAT CGATGGACAT ACCCATCTGT 
CGCGGTGACC TCATGTACTG CGTCGACATC CTCGACGCCC TTACGAAAGA CTTCTTTGCG 
CGGAAGGGCA ATCCGATAGA GGAGACGGGT GAGATTGGTG AGATAGCGGC CCGCCCGGAT 
ACGGAGGGCT ACGAGCCCGT CTCATCAACG CTGTGGCGTC AGCGTGAGGA GTACTGCGCC 
CGGCTAATCC AGCACGCCTG GCGAAAGCAC AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT 
GAGCCGGATA CGGATCATGG CGATGGCGGT GATCCGGATG CCGGGGACCC GGCGCCCGAT 
GAAGCAACGG ACGGCGATGC GCCCGCTGGT GGAGATGGTA GTGTTAACGG TACTGCAGAA 
GGAGCTGCCG ATGCCGATGA GAGTAATGTA AATAGTCCGG GTGAGGATGC AGCGGCGGCG 
GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG GGCACGACGA CGGCGGGAAG TCCCGGAGCG 
GGTAGCGCCG GGCGACAGAC CGCCGTTCTC GTGGAGAGCG ACGGGTTCGT GACGAAGAAC 
GGCCACAAGG TGGTCATCCA CTCGCGATCG CCGAGCATCA CGTCGCGCAC GGCGGATGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188605.1
CDS	269..6571
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201676.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201676.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTTTTCAAG CTGGCCAAAT CGTGGCCGAC GCTGAATTTG 
CTCATATCGA TTATGGGCCG CACTGTCGGC GCCCTCGGCA ATCTGACCTT CGTCCTGTGC 
ATTATTATCT TCATATTCGC GGTCATGGGC ATGCAGCTGT TTGGCAAGAA CTACACAGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GAGCATGGTG ACAACGAACT GGAGCTGGGC 
CACGACGAGA TCCTCGCCGA CGGCCTCATC AAGAAGGGGA TCAAGGAGCA GACGCAACTG 
GAGGTGGCCA TCGGGGATGG CATGGAATTC ACGATACACG GCGACATGAA GAACAACAAG 
CCGAAGAAAT CCAAATATCT AAATAACGCA ACGGACGACG ACACTGCCAG CATTAACTCA 
TATGGTAGCC ATAAGAATCG ACCATTCAAG GACGAGAGCC ACAAGGGCAG CGCCGAGACG 
ATGGAGGGCG AGGAGAAGCG CGACGCCAGC AAGGAGGATT TAGGTCTCGA CGAGGAACTG 
GACGAGGAGG GCGAATGCGA GGAGGGCCCG CTCGACGGTG ATATCATTAT TCATGCACAC 
GACGAGGATA TACTCGATGA ATATCCAGCT GATTGCTGCC CCGATTCGTA CTATAAGAAA 
TTTCCGATCT TAGCCGGTGA CGATGACTCG CCGTTCTGGC AAGGATGGGG CAATTTACGA 
CTGAAAACTT TTCAATTAAT TGAAAATAAA TATTTTGAAA CAGCTGTTAT CACTATGATT 
TTAATGAGTA GCTTAGCTTT GGCATTAGAA GATGTACATC TGCCACAAAG ACCCATACTG 
CAGGATATTT TATACTATAT GGACAGAATA TTTACGGTTA TATTCTTCTT GGAAATGTTA 
ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG TACTTCACCA ACGCGTGGTG TTGGCTCGAT 
TTCGTGATTG TCATGGTATC GCTTATCAAC TTCGTTGCTT CACTTGTTGG AGCTGGTGGT 
ATTCAAGCCT TCAAGACTAT GCGAACGTTA AGAGCACTGA GACCACTACG TGCCATGTCC 
CGTATGCAGG GCATGAGGGT CGTCGTTAAT GCGCTGGTAC AAGCTATACC GTCCATCTTC 
AATGTGCTAT TGGTGTGTCT AATATTTTGG CTAATTTTTG CCATAATGGG TGTACAGCTT 
TTTGCTGGAA AATATTTTAA GTGCGAGGAC ATGAATGGCA CGAAGCTCAG CCACGAGATC 
ATACCAAATC GCAATGCCTG CGAGAGCGAG AACTACACGT GGGTGAATTC AGCAATGAAT 
TTCGATCATG TAGGTAACGC GTATCTGTGC CTTTTCCAAG TGGCCACCTT CAAAGGCTGG 
ATACAAATCA TGAACGATGC TATCGATTCA CGAGAGGTGG ACAAGCAACC AATTCGTGAA 
ACGAACATCT ACATGTATTT ATATTTCGTA TTCTTCATCA TATTTGGATC ATTTTTCACA 
CTCAATCTGT TCATTGGTGT TATCATTGAT AATTTTAATG AGCAAAAGAA AAAAGCAGGT 
GGATCATTAG AAATGTTCAT GACAGAAGAT CAGAAAAAGT ACTATAATGC TATGAAAAAG 
ATGGGCTCTA AAAAACCATT AAAAGCCATT CCAAGACCAA GGTGGCGACC ACAAGCAATA 
GTCTTTGAAA TAGTAACCGA TAAGAAATTC GATATAATCA TTATGTTATT CATTGGTCTG 
AACATGTTCA CCATGACCCT CGATCGTTAC GATGCGTCGG ACACGTATAA CGCGGTCCTA 
GACTATCTCA ATGCGATATT CGTAGTTATT TTCAGTTCCG AATGTCTATT AAAAATATTC 
GCTTTACGAT ATCACTATTT TATTGAGCCA TGGAATTTAT TTGATGTAGT AGTTGTCATT 
TTATCCATCT TAGGTCTTGT ACTTAGCGAT ATTATCGAGA AGTACTTCGT GTCGCCGACC 
CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG GGCCGTGTCC TTCGACTGGT GAAGGGAGCC 
AAGGGCATTC GGACACTGCT CTTCGCGTTG GCCATGTCGC TGCCGGCCCT GTTCAACATC 
TGCCTGCTGC TGTTCCTGGT CATGTTCATC TTTGCCATTT TCGGCATGTC GTTCTTCATG 
CACGTGAAGG AGAAGAGCGG CATCAACGAC GTCTACAACT TCAAGACCTT TGGCCAGAGC 
ATGATCCTGC TCTTTCAGAT GTCGACGTCA GCCGGTTGGG ATGGTGTACT GGACGCCATT 
ATCAATGAGG AAGCATGCGA TCCACCCGAC AACGACAAAG GCTATCCGGG CAATTGTGGT 
TCAGCGACCG TTGGAATAAC GTTTCTCCTC TCATACCTAG TTATAAGCTT TTTGATAGTT 
ATTAATATGT ACATTGCTGT CATTCTCGAG AACTATAGTC AGGCCACCGA GGACGTGCAA 
GAGGGTCTAA CCGACGACGA CTACGACATG TACTATGAGA TCTGGCAGCA ATTCGATCCG 
GAGGGCACCC AGTACATACG CTATGATCAG CTGTCCGAAT TCCTGGACGT ACTGGAGCCC 
CCGCTGCAGA TCCACAAACC GAACAAGTAC AAGATCATAT CGATGGACAT ACCCATCTGT 
CGCGGTGACC TCATGTACTG CGTCGACATC CTCGACGCCC TTACGAAAGA CTTCTTTGCG 
CGGAAGGGCA ATCCGATAGA GGAGACGGGT GAGATTGGTG AGATAGCGGC CCGCCCGGAT 
ACGGAGGGCT ACGAGCCCGT CTCATCAACG CTGTGGCGTC AGCGTGAGGA GTACTGCGCC 
CGGCTAATCC AGCACGCCTG GCGAAAGCAC AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT 
GAGCCGGATA CGGATCATGG CGATGGCGGT GATCCGGATG CCGGGGACCC GGCGCCCGAT 
GAAGCAACGG ACGGCGATGC GCCCGCTGGT GGAGATGGTA GTGTTAACGG TACTGCAGAA 
GGAGCTGCCG ATGCCGATGA GAGTAATGTA AATAGTCCGG GTGAGGATGC AGCGGCGGCG 
GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG GGCACGACGA CGGCGGGAAG TCCCGGAGCG 
GGTAGCGCCG GGCGACAGAC CGCCGTTCTC GTGGAGAGCG ACGGGTTCGT GACGAAGAAC 
GGCCACAAGG TGGTCATCCA CTCGCGATCG CCGAGCATCA CGTCGCGCAC GGCGGATGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201690.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188619.1 Drosophila melanogaster paralytic (para), transcript variant X, mRNA.

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CloneID	OFa01818
Clone ID Related Accession (Same CDS sequence)	NM_001201690.1
Accession Version	NM_001201690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform X
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTTTTCAAG CTGGCCAAAT CGTGGCCGAC GCTGAATTTG 
CTCATATCGA TTATGGGCCG CACTGTCGGC GCCCTCGGCA ATCTGACCTT CGTCCTGTGC 
ATTATTATCT TCATATTCGC GGTCATGGGC ATGCAGCTGT TTGGCAAGAA CTACACAGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GAGCATGGTG ACAACGAACT GGAGCTGGGC 
CACGACGAGA TCCTCGCCGA CGGCCTCATC AAGAAGGGGA TCAAGGAGCA GACGCAACTG 
GAGGTGGCCA TCGGGGATGG CATGGAATTC ACGATACACG GCGACATGAA GAACAACAAG 
CCGAAGAAAT CCAAATATCT AAATAACGCA ACGATGATTG GCAACTCAAT TAACCACCAA 
GACAATAGAC TGGAACACGA GCTAAACCAT AGAGGTTTGT CCTTACAGGA CGACGACACT 
GCCAGCATTA ACTCATATGG TAGCCATAAG AATCGACCAT TCAAGGACGA GAGCCACAAG 
GGCAGCGCCG AGACGATGGA GGGCGAGGAG AAGCGCGACG CCAGCAAGGA GGATTTAGGT 
CTCGACGAGG AACTGGACGA GGAGGGCGAA TGCGAGGAGG GCCCGCTCGA CGGTGATATC 
ATTATTCATG CACACGACGA GGATATACTC GATGAATATC CAGCTGATTG CTGCCCCGAT 
TCGTACTATA AGAAATTTCC GATCTTAGCC GGTGACGATG ACTCGCCGTT CTGGCAAGGA 
TGGGGCAATT TACGACTGAA AACTTTTCAA TTAATTGAAA ATAAATATTT TGAAACAGCT 
GTTATCACTA TGATTTTAAT GAGTAGCTTA GCTTTGGCAT TAGAAGATGT ACATCTGCCA 
CAAAGACCCA TACTGCAGGA TATTTTATAC TATATGGACA GAATATTTAC GGTTATATTC 
TTCTTGGAAA TGTTAATCAA GTGGTTGGCG CTCGGCTTCA AAGTGTACTT CACCAACGCG 
TGGTGTTGGC TCGATTTCGT GATTGTCATG GTATCGCTTA TCAACTTCGT TGCTTCACTT 
GTTGGAGCTG GTGGTATTCA AGCCTTCAAG ACTATGCGAA CGTTAAGAGC ACTGAGACCA 
CTACGTGCCA TGTCCCGTAT GCAGGGCATG AGGGTCGTCG TTAATGCGCT GGTACAAGCT 
ATACCGTCCA TCTTCAATGT GCTATTGGTG TGTCTAATAT TTTGGCTAAT TTTTGCCATA 
ATGGGTGTAC AGCTTTTTGC TGGAAAATAT TTTAAGTGCG AGGACATGAA TGGCACGAAG 
CTCAGCCACG AGATCATACC AAATCGCAAT GCCTGCGAGA GCGAGAACTA CACGTGGGTG 
AATTCAGCAA TGAATTTCGA TCATGTAGGT AACGCGTATC TGTGCCTTTT CCAAGTGGCC 
ACCTTCAAAG GCTGGATACA AATCATGAAC GATGCTATCG ATTCACGAGA GGTGGACAAG 
CAACCAATTC GTGAAACGAA CATCTACATG TATTTATATT TCGTATTCTT CATCATATTT 
GGATCATTTT TCACACTCAA TCTGTTCATT GGTGTTATCA TTGATAATTT TAATGAGCAA 
AAGAAAAAAG CAGGTGGATC ATTAGAAATG TTCATGACAG AAGATCAGAA AAAGTACTAT 
AATGCTATGA AAAAGATGGG CTCTAAAAAA CCATTAAAAG CCATTCCAAG ACCAAGGTGG 
CGACCACAAG CAATAGTCTT TGAAATAGTA ACCGATAAGA AATTCGATAT AATCATTATG 
TTATTCATTG GTCTGAACAT GTTCACCATG ACCCTCGATC GTTACGATGC GTCGGACACG 
TATAACGCGG TCCTAGACTA TCTCAATGCG ATATTCGTAG TTATTTTCAG TTCCGAATGT 
CTATTAAAAA TATTCGCTTT ACGATATCAC TATTTTATTG AGCCATGGAA TTTATTTGAT 
GTAGTAGTTG TCATTTTATC CATCTTAGGT CTTGTACTTA GCGATATTAT CGAGAAGTAC 
TTCGTGTCGC CGACCCTGCT CCGAGTGGTG CGTGTGGCGA AAGTGGGCCG TGTCCTTCGA 
CTGGTGAAGG GAGCCAAGGG CATTCGGACA CTGCTCTTCG CGTTGGCCAT GTCGCTGCCG 
GCCCTGTTCA ACATCTGCCT GCTGCTGTTC CTGGTCATGT TCATCTTTGC CATTTTCGGC 
ATGTCGTTCT TCATGCACGT GAAGGAGAAG AGCGGCATCA ACGACGTCTA CAACTTCAAG 
ACCTTTGGCC AGAGCATGAT CCTGCTCTTT CAGATGTCGA CGTCAGCCGG TTGGGATGGT 
GTACTGGACG CCATTATCAA TGAGGAAGCA TGCGATCCAC CCGACAACGA CAAAGGCTAT 
CCGGGCAATT GTGGTTCAGC GACCGTTGGA ATAACGTTTC TCCTCTCATA CCTAGTTATA 
AGCTTTTTGA TAGTTATTAA TATGTACATT GCTGTCATTC TCGAGAACTA TAGTCAGGCC 
ACCGAGGACG TGCAAGAGGG TCTAACCGAC GACGACTACG ACATGTACTA TGAGATCTGG 
CAGCAATTCG ATCCGGAGGG CACCCAGTAC ATACGCTATG ATCAGCTGTC CGAATTCCTG 
GACGTACTGG AGCCCCCGCT GCAGATCCAC AAACCGAACA AGTACAAGAT CATATCGATG 
GACATACCCA TCTGTCGCGG TGACCTCATG TACTGCGTCG ACATCCTCGA CGCCCTTACG 
AAAGACTTCT TTGCGCGGAA GGGCAATCCG ATAGAGGAGA CGGGTGAGAT TGGTGAGATA 
GCGGCCCGCC CGGATACGGA GGGCTACGAG CCCGTCTCAT CAACGCTGTG GCGTCAGCGT 
GAGGAGTACT GCGCCCGGCT AATCCAGCAC GCCTGGCGAA AGCACAAGGC GCGCGGCGAG 
GGAGGTGGGT CCTTTGAGCC GGATACGGAT CATGGCGATG GCGGTGATCC GGATGCCGGG 
GACCCGGCGC CCGATGAAGC AACGGACGGC GATGCGCCCG CTGGTGGAGA TGGTAGTGTT 
AACGGTACTG CAGAAGGAGC TGCCGATGCC GATGAGAGTA ATGTAAATAG TCCGGGTGAG 
GATGCAGCGG CGGCGGCAGC AGCAGCAGCA GCAGCGGCGG CGGCGGGCAC GACGACGGCG 
GGAAGTCCCG GAGCGGGTAG CGCCGGGCGA CAGACCGCCG TTCTCGTGGA GAGCGACGGG 
TTCGTGACGA AGAACGGCCA CAAGGTGGTC ATCCACTCGC GATCGCCGAG CATCACGTCG 
CGCACGGCGG ATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188619.1
CDS	269..6646
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATMIGNSINHQDNRLEHELNHRGLSLQDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201690.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant X, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201690.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTTTTCAAG CTGGCCAAAT CGTGGCCGAC GCTGAATTTG 
CTCATATCGA TTATGGGCCG CACTGTCGGC GCCCTCGGCA ATCTGACCTT CGTCCTGTGC 
ATTATTATCT TCATATTCGC GGTCATGGGC ATGCAGCTGT TTGGCAAGAA CTACACAGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GAGCATGGTG ACAACGAACT GGAGCTGGGC 
CACGACGAGA TCCTCGCCGA CGGCCTCATC AAGAAGGGGA TCAAGGAGCA GACGCAACTG 
GAGGTGGCCA TCGGGGATGG CATGGAATTC ACGATACACG GCGACATGAA GAACAACAAG 
CCGAAGAAAT CCAAATATCT AAATAACGCA ACGATGATTG GCAACTCAAT TAACCACCAA 
GACAATAGAC TGGAACACGA GCTAAACCAT AGAGGTTTGT CCTTACAGGA CGACGACACT 
GCCAGCATTA ACTCATATGG TAGCCATAAG AATCGACCAT TCAAGGACGA GAGCCACAAG 
GGCAGCGCCG AGACGATGGA GGGCGAGGAG AAGCGCGACG CCAGCAAGGA GGATTTAGGT 
CTCGACGAGG AACTGGACGA GGAGGGCGAA TGCGAGGAGG GCCCGCTCGA CGGTGATATC 
ATTATTCATG CACACGACGA GGATATACTC GATGAATATC CAGCTGATTG CTGCCCCGAT 
TCGTACTATA AGAAATTTCC GATCTTAGCC GGTGACGATG ACTCGCCGTT CTGGCAAGGA 
TGGGGCAATT TACGACTGAA AACTTTTCAA TTAATTGAAA ATAAATATTT TGAAACAGCT 
GTTATCACTA TGATTTTAAT GAGTAGCTTA GCTTTGGCAT TAGAAGATGT ACATCTGCCA 
CAAAGACCCA TACTGCAGGA TATTTTATAC TATATGGACA GAATATTTAC GGTTATATTC 
TTCTTGGAAA TGTTAATCAA GTGGTTGGCG CTCGGCTTCA AAGTGTACTT CACCAACGCG 
TGGTGTTGGC TCGATTTCGT GATTGTCATG GTATCGCTTA TCAACTTCGT TGCTTCACTT 
GTTGGAGCTG GTGGTATTCA AGCCTTCAAG ACTATGCGAA CGTTAAGAGC ACTGAGACCA 
CTACGTGCCA TGTCCCGTAT GCAGGGCATG AGGGTCGTCG TTAATGCGCT GGTACAAGCT 
ATACCGTCCA TCTTCAATGT GCTATTGGTG TGTCTAATAT TTTGGCTAAT TTTTGCCATA 
ATGGGTGTAC AGCTTTTTGC TGGAAAATAT TTTAAGTGCG AGGACATGAA TGGCACGAAG 
CTCAGCCACG AGATCATACC AAATCGCAAT GCCTGCGAGA GCGAGAACTA CACGTGGGTG 
AATTCAGCAA TGAATTTCGA TCATGTAGGT AACGCGTATC TGTGCCTTTT CCAAGTGGCC 
ACCTTCAAAG GCTGGATACA AATCATGAAC GATGCTATCG ATTCACGAGA GGTGGACAAG 
CAACCAATTC GTGAAACGAA CATCTACATG TATTTATATT TCGTATTCTT CATCATATTT 
GGATCATTTT TCACACTCAA TCTGTTCATT GGTGTTATCA TTGATAATTT TAATGAGCAA 
AAGAAAAAAG CAGGTGGATC ATTAGAAATG TTCATGACAG AAGATCAGAA AAAGTACTAT 
AATGCTATGA AAAAGATGGG CTCTAAAAAA CCATTAAAAG CCATTCCAAG ACCAAGGTGG 
CGACCACAAG CAATAGTCTT TGAAATAGTA ACCGATAAGA AATTCGATAT AATCATTATG 
TTATTCATTG GTCTGAACAT GTTCACCATG ACCCTCGATC GTTACGATGC GTCGGACACG 
TATAACGCGG TCCTAGACTA TCTCAATGCG ATATTCGTAG TTATTTTCAG TTCCGAATGT 
CTATTAAAAA TATTCGCTTT ACGATATCAC TATTTTATTG AGCCATGGAA TTTATTTGAT 
GTAGTAGTTG TCATTTTATC CATCTTAGGT CTTGTACTTA GCGATATTAT CGAGAAGTAC 
TTCGTGTCGC CGACCCTGCT CCGAGTGGTG CGTGTGGCGA AAGTGGGCCG TGTCCTTCGA 
CTGGTGAAGG GAGCCAAGGG CATTCGGACA CTGCTCTTCG CGTTGGCCAT GTCGCTGCCG 
GCCCTGTTCA ACATCTGCCT GCTGCTGTTC CTGGTCATGT TCATCTTTGC CATTTTCGGC 
ATGTCGTTCT TCATGCACGT GAAGGAGAAG AGCGGCATCA ACGACGTCTA CAACTTCAAG 
ACCTTTGGCC AGAGCATGAT CCTGCTCTTT CAGATGTCGA CGTCAGCCGG TTGGGATGGT 
GTACTGGACG CCATTATCAA TGAGGAAGCA TGCGATCCAC CCGACAACGA CAAAGGCTAT 
CCGGGCAATT GTGGTTCAGC GACCGTTGGA ATAACGTTTC TCCTCTCATA CCTAGTTATA 
AGCTTTTTGA TAGTTATTAA TATGTACATT GCTGTCATTC TCGAGAACTA TAGTCAGGCC 
ACCGAGGACG TGCAAGAGGG TCTAACCGAC GACGACTACG ACATGTACTA TGAGATCTGG 
CAGCAATTCG ATCCGGAGGG CACCCAGTAC ATACGCTATG ATCAGCTGTC CGAATTCCTG 
GACGTACTGG AGCCCCCGCT GCAGATCCAC AAACCGAACA AGTACAAGAT CATATCGATG 
GACATACCCA TCTGTCGCGG TGACCTCATG TACTGCGTCG ACATCCTCGA CGCCCTTACG 
AAAGACTTCT TTGCGCGGAA GGGCAATCCG ATAGAGGAGA CGGGTGAGAT TGGTGAGATA 
GCGGCCCGCC CGGATACGGA GGGCTACGAG CCCGTCTCAT CAACGCTGTG GCGTCAGCGT 
GAGGAGTACT GCGCCCGGCT AATCCAGCAC GCCTGGCGAA AGCACAAGGC GCGCGGCGAG 
GGAGGTGGGT CCTTTGAGCC GGATACGGAT CATGGCGATG GCGGTGATCC GGATGCCGGG 
GACCCGGCGC CCGATGAAGC AACGGACGGC GATGCGCCCG CTGGTGGAGA TGGTAGTGTT 
AACGGTACTG CAGAAGGAGC TGCCGATGCC GATGAGAGTA ATGTAAATAG TCCGGGTGAG 
GATGCAGCGG CGGCGGCAGC AGCAGCAGCA GCAGCGGCGG CGGCGGGCAC GACGACGGCG 
GGAAGTCCCG GAGCGGGTAG CGCCGGGCGA CAGACCGCCG TTCTCGTGGA GAGCGACGGG 
TTCGTGACGA AGAACGGCCA CAAGGTGGTC ATCCACTCGC GATCGCCGAG CATCACGTCG 
CGCACGGCGG ATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201691.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188620.1 Drosophila melanogaster paralytic (para), transcript variant Y, mRNA.

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CloneID	OFa01819
Clone ID Related Accession (Same CDS sequence)	NM_001201691.1
Accession Version	NM_001201691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform Y
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTTTTCAAG CTGGCCAAAT CGTGGCCGAC GCTGAATTTG 
CTCATATCGA TTATGGGCCG CACTGTCGGC GCCCTCGGCA ATCTGACCTT CGTCCTGTGC 
ATTATTATCT TCATATTCGC GGTCATGGGC ATGCAGCTGT TTGGCAAGAA CTACACAGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GGTGAGAGGA CCAACCAGAT CAGTTGGATT 
TGGAGCGAAG AGCATGGTGA CAACGAACTG GAGCTGGGCC ACGACGAGAT CCTCGCCGAC 
GGCCTCATCA AGAAGGGGAT CAAGGAGCAG ACGCAACTGG AGGTGGCCAT CGGGGATGGC 
ATGGAATTCA CGATACACGG CGACATGAAG AACAACAAGC CGAAGAAATC CAAATATCTA 
AATAACGCAA CGATGATTGG CAACTCAATT AACCACCAAG ACAATAGACT GGAACACGAG 
CTAAACCATA GAGGTTTGTC CTTACAGGAC GACGACACTG CCAGCATTAA CTCATATGGT 
AGCCATAAGA ATCGACCATT CAAGGACGAG AGCCACAAGG GCAGCGCCGA GACGATGGAG 
GGCGAGGAGA AGCGCGACGC CAGCAAGGAG GATTTAGGTC TCGACGAGGA ACTGGACGAG 
GAGGGCGAAT GCGAGGAGGG CCCGCTCGAC GGTGATATCA TTATTCATGC ACACGACGAG 
GATATACTCG ATGAATATCC AGCTGATTGC TGCCCCGATT CGTACTATAA GAAATTTCCG 
ATCTTAGCCG GTGACGATGA CTCGCCGTTC TGGCAAGGAT GGGGCAATTT ACGACTGAAA 
ACTTTTCAAT TAATTGAAAA TAAATATTTT GAAACAGCTG TTATCACTAT GATTTTAATG 
AGTAGCTTAG CTTTGGCATT AGAAGATGTA CATCTGCCAC AAAGACCCAT ACTGCAGGAT 
ATTTTATACT ATATGGACAG AATATTTACG GTTATATTCT TCTTGGAAAT GTTAATCAAG 
TGGTTGGCGC TCGGCTTCAA AGTGTACTTC ACCAACGCGT GGTGTTGGCT CGATTTCGTG 
ATTGTCATGG TATCGCTTAT CAACTTCGTT GCTTCACTTG TTGGAGCTGG TGGTATTCAA 
GCCTTCAAGA CTATGCGAAC GTTAAGAGCA CTGAGACCAC TACGTGCCAT GTCCCGTATG 
CAGGGCATGA GGGTCGTCGT TAATGCGCTG GTACAAGCTA TACCGTCCAT CTTCAATGTG 
CTATTGGTGT GTCTAATATT TTGGCTAATT TTTGCCATAA TGGGTGTACA GCTTTTTGCT 
GGAAAATATT TTAAGTGCGA GGACATGAAT GGCACGAAGC TCAGCCACGA GATCATACCA 
AATCGCAATG CCTGCGAGAG CGAGAACTAC ACGTGGGTGA ATTCAGCAAT GAATTTCGAT 
CATGTAGGTA ACGCGTATCT GTGCCTTTTC CAAGTGGCCA CCTTCAAAGG CTGGATACAA 
ATCATGAACG ATGCTATCGA TTCACGAGAG GTGGACAAGC AACCAATTCG TGAAACGAAC 
ATCTACATGT ATTTATATTT CGTATTCTTC ATCATATTTG GATCATTTTT CACACTCAAT 
CTGTTCATTG GTGTTATCAT TGATAATTTT AATGAGCAAA AGAAAAAAGC AGGTGGATCA 
TTAGAAATGT TCATGACAGA AGATCAGAAA AAGTACTATA ATGCTATGAA AAAGATGGGC 
TCTAAAAAAC CATTAAAAGC CATTCCAAGA CCAAGGTGGC GACCACAAGC AATAGTCTTT 
GAAATAGTAA CCGATAAGAA ATTCGATATA ATCATTATGT TATTCATTGG TCTGAACATG 
TTCACCATGA CCCTCGATCG TTACGATGCG TCGGACACGT ATAACGCGGT CCTAGACTAT 
CTCAATGCGA TATTCGTAGT TATTTTCAGT TCCGAATGTC TATTAAAAAT ATTCGCTTTA 
CGATATCACT ATTTTATTGA GCCATGGAAT TTATTTGATG TAGTAGTTGT CATTTTATCC 
ATCTTAGGTC TTGTACTTAG CGATATTATC GAGAAGTACT TCGTGTCGCC GACCCTGCTC 
CGAGTGGTGC GTGTGGCGAA AGTGGGCCGT GTCCTTCGAC TGGTGAAGGG AGCCAAGGGC 
ATTCGGACAC TGCTCTTCGC GTTGGCCATG TCGCTGCCGG CCCTGTTCAA CATCTGCCTG 
CTGCTGTTCC TGGTCATGTT CATCTTTGCC ATTTTCGGCA TGTCGTTCTT CATGCACGTG 
AAGGAGAAGA GCGGCATCAA CGACGTCTAC AACTTCAAGA CCTTTGGCCA GAGCATGATC 
CTGCTCTTTC AGATGTCGAC GTCAGCCGGT TGGGATGGTG TACTGGACGC CATTATCAAT 
GAGGAAGCAT GCGATCCACC CGACAACGAC AAAGGCTATC CGGGCAATTG TGGTTCAGCG 
ACCGTTGGAA TAACGTTTCT CCTCTCATAC CTAGTTATAA GCTTTTTGAT AGTTATTAAT 
ATGTACATTG CTGTCATTCT CGAGAACTAT AGTCAGGCCA CCGAGGACGT GCAAGAGGGT 
CTAACCGACG ACGACTACGA CATGTACTAT GAGATCTGGC AGCAATTCGA TCCGGAGGGC 
ACCCAGTACA TACGCTATGA TCAGCTGTCC GAATTCCTGG ACGTACTGGA GCCCCCGCTG 
CAGATCCACA AACCGAACAA GTACAAGATC ATATCGATGG ACATACCCAT CTGTCGCGGT 
GACCTCATGT ACTGCGTCGA CATCCTCGAC GCCCTTACGA AAGACTTCTT TGCGCGGAAG 
GGCAATCCGA TAGAGGAGAC GGGTGAGATT GGTGAGATAG CGGCCCGCCC GGATACGGAG 
GGCTACGAGC CCGTCTCATC AACGCTGTGG CGTCAGCGTG AGGAGTACTG CGCCCGGCTA 
ATCCAGCACG CCTGGCGAAA GCACAAGGCG CGCGGCGAGG GAGGTGGGTC CTTTGAGCCG 
GATACGGATC ATGGCGATGG CGGTGATCCG GATGCCGGGG ACCCGGCGCC CGATGAAGCA 
ACGGACGGCG ATGCGCCCGC TGGTGGAGAT GGTAGTGTTA ACGGTACTGC AGAAGGAGCT 
GCCGATGCCG ATGAGAGTAA TGTAAATAGT CCGGGTGAGG ATGCAGCGGC GGCGGCAGCA 
GCAGCAGCAG CAGCGGCGGC GGCGGGCACG ACGACGGCGG GAAGTCCCGG AGCGGGTAGC 
GCCGGGCGAC AGACCGCCGT TCTCGTGGAG AGCGACGGGT TCGTGACGAA GAACGGCCAC 
AAGGTGGTCA TCCACTCGCG ATCGCCGAGC ATCACGTCGC GCACGGCGGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188620.1
CDS	269..6685
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATMIGNSINHQDNRLEHELNHRGLSLQDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201691.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant Y, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201691.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGAGG ACGATGACGA GGATGGGCCG ACGTTCAAAG ACAAGGCACT CGAAGTGATC 
CTCAAAGGCA TCGATGTGTT TTGTGTGTGG GACTGTTGCT GGGTTTGGTT GAAATTTCAG 
GAGTGGGTAT CGCTCATCGT CTTCGATCCC TTCGTCGAGC TCTTCATCAC GCTGTGCATT 
GTGGTCAACA CGATGTTCAT GGCAATGGAT CACCACGATA TGAACAAGGA GATGGAACGC 
GTGCTCAAGA GTGGCAACTA TTTCTTCACC GCCACCTTTG CCATCGAGGC CACCATGAAG 
CTAATGGCCA TGAGCCCCAA GTACTATTTC CAGGAGGGCT GGAACATCTT CGACTTCATT 
ATCGTGGCCC TATCGCTATT GGAACTGGGA CTCGAGGGTG TCCAGGGTCT GTCCGTATTG 
CGTTCCTTTC GATTGCTGCG TGTTTTCAAG CTGGCCAAAT CGTGGCCGAC GCTGAATTTG 
CTCATATCGA TTATGGGCCG CACTGTCGGC GCCCTCGGCA ATCTGACCTT CGTCCTGTGC 
ATTATTATCT TCATATTCGC GGTCATGGGC ATGCAGCTGT TTGGCAAGAA CTACACAGAT 
CACAAGGACC GCTTTCCGGA TGGCGACCTG CCGCGCTGGA ACTTCACCGA CTTTATGCAC 
AGCTTCATGA TCGTGTTCCG GGTGCTCTGC GGAGAATGGA TCGAGTCCAT GTGGGACTGC 
ATGTACGTGG GCGATGTCTC GTGCATTCCC TTCTTCTTGG CCACCGTTGT CATCGGCAAT 
CTTGTGGTAC TTAACCTTTT CTTAGCCTTG CTTTTGTCCA ATTTTGGCTC ATCTAGCTTA 
TCAGCGCCGA CTGCCGATAA CGATACGAAT AAAATAGCCG AGGCCTTCAA TCGAATTGGC 
CGATTTAAAA GTTGGGTTAA GCGTAATATT GCTGATTGTT TCAAGTTAAT ACGTAACAAA 
TTGACAAATC AAATAAGTGA TCAACCATCA GGTGAGAGGA CCAACCAGAT CAGTTGGATT 
TGGAGCGAAG AGCATGGTGA CAACGAACTG GAGCTGGGCC ACGACGAGAT CCTCGCCGAC 
GGCCTCATCA AGAAGGGGAT CAAGGAGCAG ACGCAACTGG AGGTGGCCAT CGGGGATGGC 
ATGGAATTCA CGATACACGG CGACATGAAG AACAACAAGC CGAAGAAATC CAAATATCTA 
AATAACGCAA CGATGATTGG CAACTCAATT AACCACCAAG ACAATAGACT GGAACACGAG 
CTAAACCATA GAGGTTTGTC CTTACAGGAC GACGACACTG CCAGCATTAA CTCATATGGT 
AGCCATAAGA ATCGACCATT CAAGGACGAG AGCCACAAGG GCAGCGCCGA GACGATGGAG 
GGCGAGGAGA AGCGCGACGC CAGCAAGGAG GATTTAGGTC TCGACGAGGA ACTGGACGAG 
GAGGGCGAAT GCGAGGAGGG CCCGCTCGAC GGTGATATCA TTATTCATGC ACACGACGAG 
GATATACTCG ATGAATATCC AGCTGATTGC TGCCCCGATT CGTACTATAA GAAATTTCCG 
ATCTTAGCCG GTGACGATGA CTCGCCGTTC TGGCAAGGAT GGGGCAATTT ACGACTGAAA 
ACTTTTCAAT TAATTGAAAA TAAATATTTT GAAACAGCTG TTATCACTAT GATTTTAATG 
AGTAGCTTAG CTTTGGCATT AGAAGATGTA CATCTGCCAC AAAGACCCAT ACTGCAGGAT 
ATTTTATACT ATATGGACAG AATATTTACG GTTATATTCT TCTTGGAAAT GTTAATCAAG 
TGGTTGGCGC TCGGCTTCAA AGTGTACTTC ACCAACGCGT GGTGTTGGCT CGATTTCGTG 
ATTGTCATGG TATCGCTTAT CAACTTCGTT GCTTCACTTG TTGGAGCTGG TGGTATTCAA 
GCCTTCAAGA CTATGCGAAC GTTAAGAGCA CTGAGACCAC TACGTGCCAT GTCCCGTATG 
CAGGGCATGA GGGTCGTCGT TAATGCGCTG GTACAAGCTA TACCGTCCAT CTTCAATGTG 
CTATTGGTGT GTCTAATATT TTGGCTAATT TTTGCCATAA TGGGTGTACA GCTTTTTGCT 
GGAAAATATT TTAAGTGCGA GGACATGAAT GGCACGAAGC TCAGCCACGA GATCATACCA 
AATCGCAATG CCTGCGAGAG CGAGAACTAC ACGTGGGTGA ATTCAGCAAT GAATTTCGAT 
CATGTAGGTA ACGCGTATCT GTGCCTTTTC CAAGTGGCCA CCTTCAAAGG CTGGATACAA 
ATCATGAACG ATGCTATCGA TTCACGAGAG GTGGACAAGC AACCAATTCG TGAAACGAAC 
ATCTACATGT ATTTATATTT CGTATTCTTC ATCATATTTG GATCATTTTT CACACTCAAT 
CTGTTCATTG GTGTTATCAT TGATAATTTT AATGAGCAAA AGAAAAAAGC AGGTGGATCA 
TTAGAAATGT TCATGACAGA AGATCAGAAA AAGTACTATA ATGCTATGAA AAAGATGGGC 
TCTAAAAAAC CATTAAAAGC CATTCCAAGA CCAAGGTGGC GACCACAAGC AATAGTCTTT 
GAAATAGTAA CCGATAAGAA ATTCGATATA ATCATTATGT TATTCATTGG TCTGAACATG 
TTCACCATGA CCCTCGATCG TTACGATGCG TCGGACACGT ATAACGCGGT CCTAGACTAT 
CTCAATGCGA TATTCGTAGT TATTTTCAGT TCCGAATGTC TATTAAAAAT ATTCGCTTTA 
CGATATCACT ATTTTATTGA GCCATGGAAT TTATTTGATG TAGTAGTTGT CATTTTATCC 
ATCTTAGGTC TTGTACTTAG CGATATTATC GAGAAGTACT TCGTGTCGCC GACCCTGCTC 
CGAGTGGTGC GTGTGGCGAA AGTGGGCCGT GTCCTTCGAC TGGTGAAGGG AGCCAAGGGC 
ATTCGGACAC TGCTCTTCGC GTTGGCCATG TCGCTGCCGG CCCTGTTCAA CATCTGCCTG 
CTGCTGTTCC TGGTCATGTT CATCTTTGCC ATTTTCGGCA TGTCGTTCTT CATGCACGTG 
AAGGAGAAGA GCGGCATCAA CGACGTCTAC AACTTCAAGA CCTTTGGCCA GAGCATGATC 
CTGCTCTTTC AGATGTCGAC GTCAGCCGGT TGGGATGGTG TACTGGACGC CATTATCAAT 
GAGGAAGCAT GCGATCCACC CGACAACGAC AAAGGCTATC CGGGCAATTG TGGTTCAGCG 
ACCGTTGGAA TAACGTTTCT CCTCTCATAC CTAGTTATAA GCTTTTTGAT AGTTATTAAT 
ATGTACATTG CTGTCATTCT CGAGAACTAT AGTCAGGCCA CCGAGGACGT GCAAGAGGGT 
CTAACCGACG ACGACTACGA CATGTACTAT GAGATCTGGC AGCAATTCGA TCCGGAGGGC 
ACCCAGTACA TACGCTATGA TCAGCTGTCC GAATTCCTGG ACGTACTGGA GCCCCCGCTG 
CAGATCCACA AACCGAACAA GTACAAGATC ATATCGATGG ACATACCCAT CTGTCGCGGT 
GACCTCATGT ACTGCGTCGA CATCCTCGAC GCCCTTACGA AAGACTTCTT TGCGCGGAAG 
GGCAATCCGA TAGAGGAGAC GGGTGAGATT GGTGAGATAG CGGCCCGCCC GGATACGGAG 
GGCTACGAGC CCGTCTCATC AACGCTGTGG CGTCAGCGTG AGGAGTACTG CGCCCGGCTA 
ATCCAGCACG CCTGGCGAAA GCACAAGGCG CGCGGCGAGG GAGGTGGGTC CTTTGAGCCG 
GATACGGATC ATGGCGATGG CGGTGATCCG GATGCCGGGG ACCCGGCGCC CGATGAAGCA 
ACGGACGGCG ATGCGCCCGC TGGTGGAGAT GGTAGTGTTA ACGGTACTGC AGAAGGAGCT 
GCCGATGCCG ATGAGAGTAA TGTAAATAGT CCGGGTGAGG ATGCAGCGGC GGCGGCAGCA 
GCAGCAGCAG CAGCGGCGGC GGCGGGCACG ACGACGGCGG GAAGTCCCGG AGCGGGTAGC 
GCCGGGCGAC AGACCGCCGT TCTCGTGGAG AGCGACGGGT TCGTGACGAA GAACGGCCAC 
AAGGTGGTCA TCCACTCGCG ATCGCCGAGC ATCACGTCGC GCACGGCGGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201671.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188600.1 Drosophila melanogaster paralytic (para), transcript variant E, mRNA.

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CloneID	OFa01820
Clone ID Related Accession (Same CDS sequence)	NM_001201671.1
Accession Version	NM_001201671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGAGCAT 
GGTGACAACG AACTGGAGCT GGGCCACGAC GAGATCCTCG CCGACGGCCT CATCAAGAAG 
GGGATCAAGG AGCAGACGCA ACTGGAGGTG GCCATCGGGG ATGGCATGGA ATTCACGATA 
CACGGCGACA TGAAGAACAA CAAGCCGAAG AAATCCAAAT ATCTAAATAA CGCAACGGAC 
GACGACACTG CCAGCATTAA CTCATATGGT AGCCATAAGA ATCGACCATT CAAGGACGAG 
AGCCACAAGG GCAGCGCCGA GACGATGGAG GGCGAGGAGA AGCGCGACGC CAGCAAGGAG 
GATTTAGGTC TCGACGAGGA ACTGGACGAG GAGGGCGAAT GCGAGGAGGG CCCGCTCGAC 
GGTGATATCA TTATTCATGC ACACGACGAG GATATACTCG ATGAATATCC AGCTGATTGC 
TGCCCCGATT CGTACTATAA GAAATTTCCG ATCTTAGCCG GTGACGATGA CTCGCCGTTC 
TGGCAAGGAT GGGGCAATTT ACGACTGAAA ACTTTTCAAT TAATTGAAAA TAAATATTTT 
GAAACAGCTG TTATCACTAT GATTTTAATG AGTAGCTTAG CTTTGGCATT AGAAGATGTA 
CATCTGCCAC AAAGACCCAT ACTGCAGGAT ATTTTATACT ATATGGACAG AATATTTACG 
GTTATATTCT TCTTGGAAAT GTTAATCAAG TGGTTGGCGC TCGGCTTCAA AGTGTACTTC 
ACCAACGCGT GGTGTTGGCT CGATTTCGTG ATTGTCATGG TATCGCTTAT CAACTTCGTT 
GCTTCACTTG TTGGAGCTGG TGGTATTCAA GCCTTCAAGA CTATGCGAAC GTTAAGAGCA 
CTGAGACCAC TACGTGCCAT GTCCCGTATG CAGGGCATGA GGGTCGTCGT TAATGCGCTG 
GTACAAGCTA TACCGTCCAT CTTCAATGTG CTATTGGTGT GTCTAATATT TTGGCTAATT 
TTTGCCATAA TGGGTGTACA GCTTTTTGCT GGAAAATATT TTAAGTGCGA GGACATGAAT 
GGCACGAAGC TCAGCCACGA GATCATACCA AATCGCAATG CCTGCGAGAG CGAGAACTAC 
ACGTGGGTGA ATTCAGCAAT GAATTTCGAT CATGTAGGTA ACGCGTATCT GTGCCTTTTC 
CAAGTGGCCA CCTTCAAAGG CTGGATACAA ATCATGAACG ATGCTATCGA TTCACGAGAG 
GTGGACAAGC AACCAATTCG TGAAACGAAC ATCTACATGT ATTTATATTT CGTATTCTTC 
ATCATATTTG GATCATTTTT CACACTCAAT CTGTTCATTG GTGTTATCAT TGATAATTTT 
AATGAGCAAA AGAAAAAAGC AGGTGGATCA TTAGAAATGT TCATGACAGA AGATCAGAAA 
AAGTACTATA ATGCTATGAA AAAGATGGGC TCTAAAAAAC CATTAAAAGC CATTCCAAGA 
CCAAGGTGGC GACCACAAGC AATAGTCTTT GAAATAGTAA CCGATAAGAA ATTCGATATA 
ATCATTATGT TATTCATTGG TCTGAACATG TTCACCATGA CCCTCGATCG TTACGATGCG 
TCGGACACGT ATAACGCGGT CCTAGACTAT CTCAATGCGA TATTCGTAGT TATTTTCAGT 
TCCGAATGTC TATTAAAAAT ATTCGCTTTA CGATATCACT ATTTTATTGA GCCATGGAAT 
TTATTTGATG TAGTAGTTGT CATTTTATCC ATCTTAGGTC TTGTACTTAG CGATATTATC 
GAGAAGTACT TCGTGTCGCC GACCCTGCTC CGAGTGGTGC GTGTGGCGAA AGTGGGCCGT 
GTCCTTCGAC TGGTGAAGGG AGCCAAGGGC ATTCGGACAC TGCTCTTCGC GTTGGCCATG 
TCGCTGCCGG CCCTGTTCAA CATCTGCCTG CTGCTGTTCC TGGTCATGTT CATCTTTGCC 
ATTTTCGGCA TGTCGTTCTT CATGCACGTG AAGGAGAAGA GCGGCATCAA CGACGTCTAC 
AACTTCAAGA CCTTTGGCCA GAGCATGATC CTGCTCTTTC AGATGTCGAC GTCAGCCGGT 
TGGGATGGTG TACTGGACGC CATTATCAAT GAGGAAGCAT GCGATCCACC CGACAACGAC 
AAAGGCTATC CGGGCAATTG TGGTTCAGCG ACCGTTGGAA TAACGTTTCT CCTCTCATAC 
CTAGTTATAA GCTTTTTGAT AGTTATTAAT ATGTACATTG CTGTCATTCT CGAGAACTAT 
AGTCAGGCCA CCGAGGACGT GCAAGAGGGT CTAACCGACG ACGACTACGA CATGTACTAT 
GAGATCTGGC AGCAATTCGA TCCGGAGGGC ACCCAGTACA TACGCTATGA TCAGCTGTCC 
GAATTCCTGG ACGTACTGGA GCCCCCGCTG CAGATCCACA AACCGAACAA GTACAAGATC 
ATATCGATGG ACATACCCAT CTGTCGCGGT GACCTCATGT ACTGCGTCGA CATCCTCGAC 
GCCCTTACGA AAGACTTCTT TGCGCGGAAG GGCAATCCGA TAGAGGAGAC GGGTGAGATT 
GGTGAGATAG CGGCCCGCCC GGATACGGAG GGCTACGAGC CCGTCTCATC AACGCTGTGG 
CGTCAGCGTG AGGAGTACTG CGCCCGGCTA ATCCAGCACG CCTGGCGAAA GCACAAGGCG 
CGCGGCGAGG GAGGTGGGTC CTTTGAGCCG GATACGGATC ATGGCGATGG CGGTGATCCG 
GATGCCGGGG ACCCGGCGCC CGATGAAGCA ACGGACGGCG ATGCGCCCGC TGGTGGAGAT 
GGTAGTGTTA ACGGTACTGC AGAAGGAGCT GCCGATGCCG ATGAGAGTAA TGTAAATAGT 
CCGGGTGAGG ATGCAGCGGC GGCGGCAGCA GCAGCAGCAG CAGCGGCGGC GGCGGGCACG 
ACGACGGCGG GAAGTCCCGG AGCGGGTAGC GCCGGGCGAC AGACCGCCGT TCTCGTGGAG 
AGCGACGGGT TCGTGACGAA GAACGGCCAC AAGGTGGTCA TCCACTCGCG ATCGCCGAGC 
ATCACGTCGC GCACGGCGGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188600.1
CDS	269..6595
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201671.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201671.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGAGCAT 
GGTGACAACG AACTGGAGCT GGGCCACGAC GAGATCCTCG CCGACGGCCT CATCAAGAAG 
GGGATCAAGG AGCAGACGCA ACTGGAGGTG GCCATCGGGG ATGGCATGGA ATTCACGATA 
CACGGCGACA TGAAGAACAA CAAGCCGAAG AAATCCAAAT ATCTAAATAA CGCAACGGAC 
GACGACACTG CCAGCATTAA CTCATATGGT AGCCATAAGA ATCGACCATT CAAGGACGAG 
AGCCACAAGG GCAGCGCCGA GACGATGGAG GGCGAGGAGA AGCGCGACGC CAGCAAGGAG 
GATTTAGGTC TCGACGAGGA ACTGGACGAG GAGGGCGAAT GCGAGGAGGG CCCGCTCGAC 
GGTGATATCA TTATTCATGC ACACGACGAG GATATACTCG ATGAATATCC AGCTGATTGC 
TGCCCCGATT CGTACTATAA GAAATTTCCG ATCTTAGCCG GTGACGATGA CTCGCCGTTC 
TGGCAAGGAT GGGGCAATTT ACGACTGAAA ACTTTTCAAT TAATTGAAAA TAAATATTTT 
GAAACAGCTG TTATCACTAT GATTTTAATG AGTAGCTTAG CTTTGGCATT AGAAGATGTA 
CATCTGCCAC AAAGACCCAT ACTGCAGGAT ATTTTATACT ATATGGACAG AATATTTACG 
GTTATATTCT TCTTGGAAAT GTTAATCAAG TGGTTGGCGC TCGGCTTCAA AGTGTACTTC 
ACCAACGCGT GGTGTTGGCT CGATTTCGTG ATTGTCATGG TATCGCTTAT CAACTTCGTT 
GCTTCACTTG TTGGAGCTGG TGGTATTCAA GCCTTCAAGA CTATGCGAAC GTTAAGAGCA 
CTGAGACCAC TACGTGCCAT GTCCCGTATG CAGGGCATGA GGGTCGTCGT TAATGCGCTG 
GTACAAGCTA TACCGTCCAT CTTCAATGTG CTATTGGTGT GTCTAATATT TTGGCTAATT 
TTTGCCATAA TGGGTGTACA GCTTTTTGCT GGAAAATATT TTAAGTGCGA GGACATGAAT 
GGCACGAAGC TCAGCCACGA GATCATACCA AATCGCAATG CCTGCGAGAG CGAGAACTAC 
ACGTGGGTGA ATTCAGCAAT GAATTTCGAT CATGTAGGTA ACGCGTATCT GTGCCTTTTC 
CAAGTGGCCA CCTTCAAAGG CTGGATACAA ATCATGAACG ATGCTATCGA TTCACGAGAG 
GTGGACAAGC AACCAATTCG TGAAACGAAC ATCTACATGT ATTTATATTT CGTATTCTTC 
ATCATATTTG GATCATTTTT CACACTCAAT CTGTTCATTG GTGTTATCAT TGATAATTTT 
AATGAGCAAA AGAAAAAAGC AGGTGGATCA TTAGAAATGT TCATGACAGA AGATCAGAAA 
AAGTACTATA ATGCTATGAA AAAGATGGGC TCTAAAAAAC CATTAAAAGC CATTCCAAGA 
CCAAGGTGGC GACCACAAGC AATAGTCTTT GAAATAGTAA CCGATAAGAA ATTCGATATA 
ATCATTATGT TATTCATTGG TCTGAACATG TTCACCATGA CCCTCGATCG TTACGATGCG 
TCGGACACGT ATAACGCGGT CCTAGACTAT CTCAATGCGA TATTCGTAGT TATTTTCAGT 
TCCGAATGTC TATTAAAAAT ATTCGCTTTA CGATATCACT ATTTTATTGA GCCATGGAAT 
TTATTTGATG TAGTAGTTGT CATTTTATCC ATCTTAGGTC TTGTACTTAG CGATATTATC 
GAGAAGTACT TCGTGTCGCC GACCCTGCTC CGAGTGGTGC GTGTGGCGAA AGTGGGCCGT 
GTCCTTCGAC TGGTGAAGGG AGCCAAGGGC ATTCGGACAC TGCTCTTCGC GTTGGCCATG 
TCGCTGCCGG CCCTGTTCAA CATCTGCCTG CTGCTGTTCC TGGTCATGTT CATCTTTGCC 
ATTTTCGGCA TGTCGTTCTT CATGCACGTG AAGGAGAAGA GCGGCATCAA CGACGTCTAC 
AACTTCAAGA CCTTTGGCCA GAGCATGATC CTGCTCTTTC AGATGTCGAC GTCAGCCGGT 
TGGGATGGTG TACTGGACGC CATTATCAAT GAGGAAGCAT GCGATCCACC CGACAACGAC 
AAAGGCTATC CGGGCAATTG TGGTTCAGCG ACCGTTGGAA TAACGTTTCT CCTCTCATAC 
CTAGTTATAA GCTTTTTGAT AGTTATTAAT ATGTACATTG CTGTCATTCT CGAGAACTAT 
AGTCAGGCCA CCGAGGACGT GCAAGAGGGT CTAACCGACG ACGACTACGA CATGTACTAT 
GAGATCTGGC AGCAATTCGA TCCGGAGGGC ACCCAGTACA TACGCTATGA TCAGCTGTCC 
GAATTCCTGG ACGTACTGGA GCCCCCGCTG CAGATCCACA AACCGAACAA GTACAAGATC 
ATATCGATGG ACATACCCAT CTGTCGCGGT GACCTCATGT ACTGCGTCGA CATCCTCGAC 
GCCCTTACGA AAGACTTCTT TGCGCGGAAG GGCAATCCGA TAGAGGAGAC GGGTGAGATT 
GGTGAGATAG CGGCCCGCCC GGATACGGAG GGCTACGAGC CCGTCTCATC AACGCTGTGG 
CGTCAGCGTG AGGAGTACTG CGCCCGGCTA ATCCAGCACG CCTGGCGAAA GCACAAGGCG 
CGCGGCGAGG GAGGTGGGTC CTTTGAGCCG GATACGGATC ATGGCGATGG CGGTGATCCG 
GATGCCGGGG ACCCGGCGCC CGATGAAGCA ACGGACGGCG ATGCGCCCGC TGGTGGAGAT 
GGTAGTGTTA ACGGTACTGC AGAAGGAGCT GCCGATGCCG ATGAGAGTAA TGTAAATAGT 
CCGGGTGAGG ATGCAGCGGC GGCGGCAGCA GCAGCAGCAG CAGCGGCGGC GGCGGGCACG 
ACGACGGCGG GAAGTCCCGG AGCGGGTAGC GCCGGGCGAC AGACCGCCGT TCTCGTGGAG 
AGCGACGGGT TCGTGACGAA GAACGGCCAC AAGGTGGTCA TCCACTCGCG ATCGCCGAGC 
ATCACGTCGC GCACGGCGGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201697.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188626.1 Drosophila melanogaster paralytic (para), transcript variant AE, mRNA.

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CloneID	OFa01821
Clone ID Related Accession (Same CDS sequence)	NM_001201697.1
Accession Version	NM_001201697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AE
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGAGCAT 
GGTGACAACG AACTGGAGCT GGGCCACGAC GAGATCCTCG CCGACGGCCT CATCAAGAAG 
GGGATCAAGG AGCAGACGCA ACTGGAGGTG GCCATCGGGG ATGGCATGGA ATTCACGATA 
CACGGCGACA TGAAGAACAA CAAGCCGAAG AAATCCAAAT ATCTAAATAA CGCAACGGAC 
GACGACACTG CCAGCATTAA CTCATATGGT AGCCATAAGA ATCGACCATT CAAGGACGAG 
AGCCACAAGG GCAGCGCCGA GACGATGGAG GGCGAGGAGA AGCGCGACGC CAGCAAGGAG 
GATTTAGGTC TCGACGAGGA ACTGGACGAG GAGGGCGAAT GCGAGGAGGG CCCGCTCGAC 
GGTGATATCA TTATTCATGC ACACGACGAG GATATACTCG ATGAATATCC AGCTGATTGC 
TGCCCCGATT CGTACTATAA GAAATTTCCG ATCTTAGCCG GTGACGATGA CTCGCCGTTC 
TGGCAAGGAT GGGGCAATTT ACGACTGAAA ACTTTTCAAT TAATTGAAAA TAAATATTTT 
GAAACAGCTG TTATCACTAT GATTTTAATG AGTAGCTTAG CTTTGGCATT AGAAGATGTA 
CATCTGCCAC AAAGACCCAT ACTGCAGGAT ATTTTATACT ATATGGACAG AATATTTACG 
GTTATATTCT TCTTGGAAAT GTTAATCAAG TGGTTGGCGC TCGGCTTCAA AGTGTACTTC 
ACCAACGCGT GGTGTTGGCT CGATTTCGTG ATTGTCATGC TGTCGCTAAT TAATTTGGCC 
GCGGTCTGGT CCGGGGCCGA TGATGTGCCC GCCTTTCGCT CGATGCGAAC TCTGCGCGCC 
CTGCGACCAC TGCGGGCAGT TTCGCGCTGG GAGGGCATGA AAGTCGTCGT TAATGCGCTG 
GTACAAGCTA TACCGTCCAT CTTCAATGTG CTATTGGTGT GTCTAATATT TTGGCTAATT 
TTTGCCATAA TGGGTGTACA GCTTTTTGCT GGAAAATATT TTAAGTGCGA GGACATGAAT 
GGCACGAAGC TCAGCCACGA GATCATACCA AATCGCAATG CCTGCGAGAG CGAGAACTAC 
ACGTGGGTGA ATTCAGCAAT GAATTTCGAT CATGTAGGTA ACGCGTATCT GTGCCTTTTC 
CAAGTGGCCA CCTTCAAAGG CTGGATACAA ATCATGAACG ATGCTATCGA TTCACGAGAG 
GTGGACAAGC AACCAATTCG TGAAACGAAC ATCTACATGT ATTTATATTT CGTATTCTTC 
ATCATATTTG GATCATTTTT CACACTCAAT CTGTTCATTG GTGTTATCAT TGATAATTTT 
AATGAGCAAA AGAAAAAAGC AGGTGGATCA TTAGAAATGT TCATGACAGA AGATCAGAAA 
AAGTACTATA ATGCTATGAA AAAGATGGGC TCTAAAAAAC CATTAAAAGC CATTCCAAGA 
CCAAGGTGGC GACCACAAGC AATAGTCTTT GAAATAGTAA CCGATAAGAA ATTCGATATA 
ATCATTATGT TATTCATTGG TCTGAACATG TTCACCATGA CCCTCGATCG TTACGATGCG 
TCGGACACGT ATAACGCGGT CCTAGACTAT CTCAATGCGA TATTCGTAGT TATTTTCAGT 
TCCGAATGTC TATTAAAAAT ATTCGCTTTA CGATATCACT ATTTTATTGA GCCATGGAAT 
TTATTTGATG TAGTAGTTGT CATTTTATCC ATCTTAGGTC TTGTACTTAG CGATATTATC 
GAGAAGTACT TCGTGTCGCC GACCCTGCTC CGAGTGGTGC GTGTGGCGAA AGTGGGCCGT 
GTCCTTCGAC TGGTGAAGGG AGCCAAGGGC ATTCGGACAC TGCTCTTCGC GTTGGCCATG 
TCGCTGCCGG CCCTGTTCAA CATCTGCCTG CTGCTGTTCC TGGTCATGTT CATCTTTGCC 
ATTTTCGGCA TGTCGTTCTT CATGCACGTG AAGGAGAAGA GCGGCATCAA CGACGTCTAC 
AACTTCAAGA CCTTTGGCCA GAGCATGATC CTGCTCTTTC AGATGTCGAC GTCAGCCGGT 
TGGGATGGTG TACTGGACGC CATTATCAAT GAGGAAGCAT GCGATCCACC CGACAACGAC 
AAAGGCTATC CGGGCAATTG TGGTTCAGCG ACCGTTGGAA TAACGTTTCT CCTCTCATAC 
CTAGTTATAA GCTTTTTGAT AGTTATTAAT ATGTACATTG CTGTCATTCT CGAGAACTAT 
AGTCAGGCCA CCGAGGACGT GCAAGAGGGT CTAACCGACG ACGACTACGA CATGTACTAT 
GAGATCTGGC AGCAATTCGA TCCGGAGGGC ACCCAGTACA TACGCTATGA TCAGCTGTCC 
GAATTCCTGG ACGTACTGGA GCCCCCGCTG CAGATCCACA AACCGAACAA GTACAAGATC 
ATATCGATGG ACATACCCAT CTGTCGCGGT GACCTCATGT ACTGCGTCGA CATCCTCGAC 
GCCCTTACGA AAGACTTCTT TGCGCGGAAG GGCAATCCGA TAGAGGAGAC GGGTGAGATT 
GGTGAGATAG CGGCCCGCCC GGATACGGAG GGCTACGAGC CCGTCTCATC AACGCTGTGG 
CGTCAGCGTG AGGAGTACTG CGCCCGGCTA ATCCAGCACG CCTGGCGAAA GCACAAGGCG 
CGCGGCGAGG GAGGTGGGTC CTTTGAGCCG GATACGGATC ATGGCGATGG CGGTGATCCG 
GATGCCGGGG ACCCGGCGCC CGATGAAGCA ACGGACGGCG ATGCGCCCGC TGGTGGAGAT 
GGTAGTGTTA ACGGTACTGC AGAAGGAGCT GCCGATGCCG ATGAGAGTAA TGTAAATAGT 
CCGGGTGAGG ATGCAGCGGC GGCGGCAGCA GCAGCAGCAG CAGCGGCGGC GGCGGGCACG 
ACGACGGCGG GAAGTCCCGG AGCGGGTAGC GCCGGGCGAC AGACCGCCGT TCTCGTGGAG 
AGCGACGGGT TCGTGACGAA GAACGGCCAC AAGGTGGTCA TCCACTCGCG ATCGCCGAGC 
ATCACGTCGC GCACGGCGGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188626.1
CDS	269..6595
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201697.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AE, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201697.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGAGCAT 
GGTGACAACG AACTGGAGCT GGGCCACGAC GAGATCCTCG CCGACGGCCT CATCAAGAAG 
GGGATCAAGG AGCAGACGCA ACTGGAGGTG GCCATCGGGG ATGGCATGGA ATTCACGATA 
CACGGCGACA TGAAGAACAA CAAGCCGAAG AAATCCAAAT ATCTAAATAA CGCAACGGAC 
GACGACACTG CCAGCATTAA CTCATATGGT AGCCATAAGA ATCGACCATT CAAGGACGAG 
AGCCACAAGG GCAGCGCCGA GACGATGGAG GGCGAGGAGA AGCGCGACGC CAGCAAGGAG 
GATTTAGGTC TCGACGAGGA ACTGGACGAG GAGGGCGAAT GCGAGGAGGG CCCGCTCGAC 
GGTGATATCA TTATTCATGC ACACGACGAG GATATACTCG ATGAATATCC AGCTGATTGC 
TGCCCCGATT CGTACTATAA GAAATTTCCG ATCTTAGCCG GTGACGATGA CTCGCCGTTC 
TGGCAAGGAT GGGGCAATTT ACGACTGAAA ACTTTTCAAT TAATTGAAAA TAAATATTTT 
GAAACAGCTG TTATCACTAT GATTTTAATG AGTAGCTTAG CTTTGGCATT AGAAGATGTA 
CATCTGCCAC AAAGACCCAT ACTGCAGGAT ATTTTATACT ATATGGACAG AATATTTACG 
GTTATATTCT TCTTGGAAAT GTTAATCAAG TGGTTGGCGC TCGGCTTCAA AGTGTACTTC 
ACCAACGCGT GGTGTTGGCT CGATTTCGTG ATTGTCATGC TGTCGCTAAT TAATTTGGCC 
GCGGTCTGGT CCGGGGCCGA TGATGTGCCC GCCTTTCGCT CGATGCGAAC TCTGCGCGCC 
CTGCGACCAC TGCGGGCAGT TTCGCGCTGG GAGGGCATGA AAGTCGTCGT TAATGCGCTG 
GTACAAGCTA TACCGTCCAT CTTCAATGTG CTATTGGTGT GTCTAATATT TTGGCTAATT 
TTTGCCATAA TGGGTGTACA GCTTTTTGCT GGAAAATATT TTAAGTGCGA GGACATGAAT 
GGCACGAAGC TCAGCCACGA GATCATACCA AATCGCAATG CCTGCGAGAG CGAGAACTAC 
ACGTGGGTGA ATTCAGCAAT GAATTTCGAT CATGTAGGTA ACGCGTATCT GTGCCTTTTC 
CAAGTGGCCA CCTTCAAAGG CTGGATACAA ATCATGAACG ATGCTATCGA TTCACGAGAG 
GTGGACAAGC AACCAATTCG TGAAACGAAC ATCTACATGT ATTTATATTT CGTATTCTTC 
ATCATATTTG GATCATTTTT CACACTCAAT CTGTTCATTG GTGTTATCAT TGATAATTTT 
AATGAGCAAA AGAAAAAAGC AGGTGGATCA TTAGAAATGT TCATGACAGA AGATCAGAAA 
AAGTACTATA ATGCTATGAA AAAGATGGGC TCTAAAAAAC CATTAAAAGC CATTCCAAGA 
CCAAGGTGGC GACCACAAGC AATAGTCTTT GAAATAGTAA CCGATAAGAA ATTCGATATA 
ATCATTATGT TATTCATTGG TCTGAACATG TTCACCATGA CCCTCGATCG TTACGATGCG 
TCGGACACGT ATAACGCGGT CCTAGACTAT CTCAATGCGA TATTCGTAGT TATTTTCAGT 
TCCGAATGTC TATTAAAAAT ATTCGCTTTA CGATATCACT ATTTTATTGA GCCATGGAAT 
TTATTTGATG TAGTAGTTGT CATTTTATCC ATCTTAGGTC TTGTACTTAG CGATATTATC 
GAGAAGTACT TCGTGTCGCC GACCCTGCTC CGAGTGGTGC GTGTGGCGAA AGTGGGCCGT 
GTCCTTCGAC TGGTGAAGGG AGCCAAGGGC ATTCGGACAC TGCTCTTCGC GTTGGCCATG 
TCGCTGCCGG CCCTGTTCAA CATCTGCCTG CTGCTGTTCC TGGTCATGTT CATCTTTGCC 
ATTTTCGGCA TGTCGTTCTT CATGCACGTG AAGGAGAAGA GCGGCATCAA CGACGTCTAC 
AACTTCAAGA CCTTTGGCCA GAGCATGATC CTGCTCTTTC AGATGTCGAC GTCAGCCGGT 
TGGGATGGTG TACTGGACGC CATTATCAAT GAGGAAGCAT GCGATCCACC CGACAACGAC 
AAAGGCTATC CGGGCAATTG TGGTTCAGCG ACCGTTGGAA TAACGTTTCT CCTCTCATAC 
CTAGTTATAA GCTTTTTGAT AGTTATTAAT ATGTACATTG CTGTCATTCT CGAGAACTAT 
AGTCAGGCCA CCGAGGACGT GCAAGAGGGT CTAACCGACG ACGACTACGA CATGTACTAT 
GAGATCTGGC AGCAATTCGA TCCGGAGGGC ACCCAGTACA TACGCTATGA TCAGCTGTCC 
GAATTCCTGG ACGTACTGGA GCCCCCGCTG CAGATCCACA AACCGAACAA GTACAAGATC 
ATATCGATGG ACATACCCAT CTGTCGCGGT GACCTCATGT ACTGCGTCGA CATCCTCGAC 
GCCCTTACGA AAGACTTCTT TGCGCGGAAG GGCAATCCGA TAGAGGAGAC GGGTGAGATT 
GGTGAGATAG CGGCCCGCCC GGATACGGAG GGCTACGAGC CCGTCTCATC AACGCTGTGG 
CGTCAGCGTG AGGAGTACTG CGCCCGGCTA ATCCAGCACG CCTGGCGAAA GCACAAGGCG 
CGCGGCGAGG GAGGTGGGTC CTTTGAGCCG GATACGGATC ATGGCGATGG CGGTGATCCG 
GATGCCGGGG ACCCGGCGCC CGATGAAGCA ACGGACGGCG ATGCGCCCGC TGGTGGAGAT 
GGTAGTGTTA ACGGTACTGC AGAAGGAGCT GCCGATGCCG ATGAGAGTAA TGTAAATAGT 
CCGGGTGAGG ATGCAGCGGC GGCGGCAGCA GCAGCAGCAG CAGCGGCGGC GGCGGGCACG 
ACGACGGCGG GAAGTCCCGG AGCGGGTAGC GCCGGGCGAC AGACCGCCGT TCTCGTGGAG 
AGCGACGGGT TCGTGACGAA GAACGGCCAC AAGGTGGTCA TCCACTCGCG ATCGCCGAGC 
ATCACGTCGC GCACGGCGGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201687.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188616.1 Drosophila melanogaster paralytic (para), transcript variant U, mRNA.

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CloneID	OFa01822
Clone ID Related Accession (Same CDS sequence)	NM_001201687.1
Accession Version	NM_001201687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform U
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGCAAA 
GGCGTTTGTC GTTGTATATC TGCAGAGCAT GGTGACAACG AACTGGAGCT GGGCCACGAC 
GAGATCCTCG CCGACGGCCT CATCAAGAAG GGGATCAAGG AGCAGACGCA ACTGGAGGTG 
GCCATCGGGG ATGGCATGGA ATTCACGATA CACGGCGACA TGAAGAACAA CAAGCCGAAG 
AAATCCAAAT ATCTAAATAA CGCAACGGAC GACGACACTG CCAGCATTAA CTCATATGGT 
AGCCATAAGA ATCGACCATT CAAGGACGAG AGCCACAAGG GCAGCGCCGA GACGATGGAG 
GGCGAGGAGA AGCGCGACGC CAGCAAGGAG GATTTAGGTC TCGACGAGGA ACTGGACGAG 
GAGGGCGAAT GCGAGGAGGG CCCGCTCGAC GGTGATATCA TTATTCATGC ACACGACGAG 
GATATACTCG ATGAATATCC AGCTGATTGC TGCCCCGATT CGTACTATAA GAAATTTCCG 
ATCTTAGCCG GTGACGATGA CTCGCCGTTC TGGCAAGGAT GGGGCAATTT ACGACTGAAA 
ACTTTTCAAT TAATTGAAAA TAAATATTTT GAAACAGCTG TTATCACTAT GATTTTAATG 
AGTAGCTTAG CTTTGGCATT AGAAGATGTA CATCTGCCAC AAAGACCCAT ACTGCAGGAT 
ATTTTATACT ATATGGACAG AATATTTACG GTTATATTCT TCTTGGAAAT GTTAATCAAG 
TGGTTGGCGC TCGGCTTCAA AGTGTACTTC ACCAACGCGT GGTGTTGGCT CGATTTCGTG 
ATTGTCATGG TATCGCTTAT CAACTTCGTT GCTTCACTTG TTGGAGCTGG TGGTATTCAA 
GCCTTCAAGA CTATGCGAAC GTTAAGAGCA CTGAGACCAC TACGTGCCAT GTCCCGTATG 
CAGGGCATGA GGGTCGTCGT TAATGCGCTG GTACAAGCTA TACCGTCCAT CTTCAATGTG 
CTATTGGTGT GTCTAATATT TTGGCTAATT TTTGCCATAA TGGGTGTACA GCTTTTTGCT 
GGAAAATATT TTAAGTGCGA GGACATGAAT GGCACGAAGC TCAGCCACGA GATCATACCA 
AATCGCAATG CCTGCGAGAG CGAGAACTAC ACGTGGGTGA ATTCAGCAAT GAATTTCGAT 
CATGTAGGTA ACGCGTATCT GTGCCTTTTC CAAGTGGCCA CCTTCAAAGG CTGGATACAA 
ATCATGAACG ATGCTATCGA TTCACGAGAG GTGGACAAGC AACCAATTCG TGAAACGAAC 
ATCTACATGT ATTTATATTT CGTATTCTTC ATCATATTTG GATCATTTTT CACACTCAAT 
CTGTTCATTG GTGTTATCAT TGATAATTTT AATGAGCAAA AGAAAAAAGC AGGTGGATCA 
TTAGAAATGT TCATGACAGA AGATCAGAAA AAGTACTATA ATGCTATGAA AAAGATGGGC 
TCTAAAAAAC CATTAAAAGC CATTCCAAGA CCAAGGTGGC GACCACAAGC AATAGTCTTT 
GAAATAGTAA CCGATAAGAA ATTCGATATA ATCATTATGT TATTCATTGG TCTGAACATG 
TTCACCATGA CCCTCGATCG TTACGATGCG TCGGACACGT ATAACGCGGT CCTAGACTAT 
CTCAATGCGA TATTCGTAGT TATTTTCAGT TCCGAATGTC TATTAAAAAT ATTCGCTTTA 
CGATATCACT ATTTTATTGA GCCATGGAAT TTATTTGATG TAGTAGTTGT CATTTTATCC 
ATCTTAGGTC TTGTACTTAG CGATATTATC GAGAAGTACT TCGTGTCGCC GACCCTGCTC 
CGAGTGGTGC GTGTGGCGAA AGTGGGCCGT GTCCTTCGAC TGGTGAAGGG AGCCAAGGGC 
ATTCGGACAC TGCTCTTCGC GTTGGCCATG TCGCTGCCGG CCCTGTTCAA CATCTGCCTG 
CTGCTGTTCC TGGTCATGTT CATCTTTGCC ATTTTCGGCA TGTCGTTCTT CATGCACGTG 
AAGGAGAAGA GCGGCATCAA CGACGTCTAC AACTTCAAGA CCTTTGGCCA GAGCATGATC 
CTGCTCTTTC AGATGTCGAC GTCAGCCGGT TGGGATGGTG TACTGGACGC CATTATCAAT 
GAGGAAGCAT GCGATCCACC CGACAACGAC AAAGGCTATC CGGGCAATTG TGGTTCAGCG 
ACCGTTGGAA TAACGTTTCT CCTCTCATAC CTAGTTATAA GCTTTTTGAT AGTTATTAAT 
ATGTACATTG CTGTCATTCT CGAGAACTAT AGTCAGGCCA CCGAGGACGT GCAAGAGGGT 
CTAACCGACG ACGACTACGA CATGTACTAT GAGATCTGGC AGCAATTCGA TCCGGAGGGC 
ACCCAGTACA TACGCTATGA TCAGCTGTCC GAATTCCTGG ACGTACTGGA GCCCCCGCTG 
CAGATCCACA AACCGAACAA GTACAAGATC ATATCGATGG ACATACCCAT CTGTCGCGGT 
GACCTCATGT ACTGCGTCGA CATCCTCGAC GCCCTTACGA AAGACTTCTT TGCGCGGAAG 
GGCAATCCGA TAGAGGAGAC GGGTGAGATT GGTGAGATAG CGGCCCGCCC GGATACGGAG 
GGCTACGAGC CCGTCTCATC AACGCTGTGG CGTCAGCGTG AGGAGTACTG CGCCCGGCTA 
ATCCAGCACG CCTGGCGAAA GCACAAGGCG CGCGGCGAGG GAGGTGGGTC CTTTGAGCCG 
GATACGGATC ATGGCGATGG CGGTGATCCG GATGCCGGGG ACCCGGCGCC CGATGAAGCA 
ACGGACGGCG ATGCGCCCGC TGGTGGAGAT GGTAGTGTTA ACGGTACTGC AGAAGGAGCT 
GCCGATGCCG ATGAGAGTAA TGTAAATAGT CCGGGTGAGG ATGCAGCGGC GGCGGCAGCA 
GCAGCAGCAG CAGCGGCGGC GGCGGGCACG ACGACGGCGG GAAGTCCCGG AGCGGGTAGC 
GCCGGGCGAC AGACCGCCGT TCTCGTGGAG AGCGACGGGT TCGTGACGAA GAACGGCCAC 
AAGGTGGTCA TCCACTCGCG ATCGCCGAGC ATCACGTCGC GCACGGCGGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188616.1
CDS	269..6625
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201687.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant U, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201687.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGCAAA 
GGCGTTTGTC GTTGTATATC TGCAGAGCAT GGTGACAACG AACTGGAGCT GGGCCACGAC 
GAGATCCTCG CCGACGGCCT CATCAAGAAG GGGATCAAGG AGCAGACGCA ACTGGAGGTG 
GCCATCGGGG ATGGCATGGA ATTCACGATA CACGGCGACA TGAAGAACAA CAAGCCGAAG 
AAATCCAAAT ATCTAAATAA CGCAACGGAC GACGACACTG CCAGCATTAA CTCATATGGT 
AGCCATAAGA ATCGACCATT CAAGGACGAG AGCCACAAGG GCAGCGCCGA GACGATGGAG 
GGCGAGGAGA AGCGCGACGC CAGCAAGGAG GATTTAGGTC TCGACGAGGA ACTGGACGAG 
GAGGGCGAAT GCGAGGAGGG CCCGCTCGAC GGTGATATCA TTATTCATGC ACACGACGAG 
GATATACTCG ATGAATATCC AGCTGATTGC TGCCCCGATT CGTACTATAA GAAATTTCCG 
ATCTTAGCCG GTGACGATGA CTCGCCGTTC TGGCAAGGAT GGGGCAATTT ACGACTGAAA 
ACTTTTCAAT TAATTGAAAA TAAATATTTT GAAACAGCTG TTATCACTAT GATTTTAATG 
AGTAGCTTAG CTTTGGCATT AGAAGATGTA CATCTGCCAC AAAGACCCAT ACTGCAGGAT 
ATTTTATACT ATATGGACAG AATATTTACG GTTATATTCT TCTTGGAAAT GTTAATCAAG 
TGGTTGGCGC TCGGCTTCAA AGTGTACTTC ACCAACGCGT GGTGTTGGCT CGATTTCGTG 
ATTGTCATGG TATCGCTTAT CAACTTCGTT GCTTCACTTG TTGGAGCTGG TGGTATTCAA 
GCCTTCAAGA CTATGCGAAC GTTAAGAGCA CTGAGACCAC TACGTGCCAT GTCCCGTATG 
CAGGGCATGA GGGTCGTCGT TAATGCGCTG GTACAAGCTA TACCGTCCAT CTTCAATGTG 
CTATTGGTGT GTCTAATATT TTGGCTAATT TTTGCCATAA TGGGTGTACA GCTTTTTGCT 
GGAAAATATT TTAAGTGCGA GGACATGAAT GGCACGAAGC TCAGCCACGA GATCATACCA 
AATCGCAATG CCTGCGAGAG CGAGAACTAC ACGTGGGTGA ATTCAGCAAT GAATTTCGAT 
CATGTAGGTA ACGCGTATCT GTGCCTTTTC CAAGTGGCCA CCTTCAAAGG CTGGATACAA 
ATCATGAACG ATGCTATCGA TTCACGAGAG GTGGACAAGC AACCAATTCG TGAAACGAAC 
ATCTACATGT ATTTATATTT CGTATTCTTC ATCATATTTG GATCATTTTT CACACTCAAT 
CTGTTCATTG GTGTTATCAT TGATAATTTT AATGAGCAAA AGAAAAAAGC AGGTGGATCA 
TTAGAAATGT TCATGACAGA AGATCAGAAA AAGTACTATA ATGCTATGAA AAAGATGGGC 
TCTAAAAAAC CATTAAAAGC CATTCCAAGA CCAAGGTGGC GACCACAAGC AATAGTCTTT 
GAAATAGTAA CCGATAAGAA ATTCGATATA ATCATTATGT TATTCATTGG TCTGAACATG 
TTCACCATGA CCCTCGATCG TTACGATGCG TCGGACACGT ATAACGCGGT CCTAGACTAT 
CTCAATGCGA TATTCGTAGT TATTTTCAGT TCCGAATGTC TATTAAAAAT ATTCGCTTTA 
CGATATCACT ATTTTATTGA GCCATGGAAT TTATTTGATG TAGTAGTTGT CATTTTATCC 
ATCTTAGGTC TTGTACTTAG CGATATTATC GAGAAGTACT TCGTGTCGCC GACCCTGCTC 
CGAGTGGTGC GTGTGGCGAA AGTGGGCCGT GTCCTTCGAC TGGTGAAGGG AGCCAAGGGC 
ATTCGGACAC TGCTCTTCGC GTTGGCCATG TCGCTGCCGG CCCTGTTCAA CATCTGCCTG 
CTGCTGTTCC TGGTCATGTT CATCTTTGCC ATTTTCGGCA TGTCGTTCTT CATGCACGTG 
AAGGAGAAGA GCGGCATCAA CGACGTCTAC AACTTCAAGA CCTTTGGCCA GAGCATGATC 
CTGCTCTTTC AGATGTCGAC GTCAGCCGGT TGGGATGGTG TACTGGACGC CATTATCAAT 
GAGGAAGCAT GCGATCCACC CGACAACGAC AAAGGCTATC CGGGCAATTG TGGTTCAGCG 
ACCGTTGGAA TAACGTTTCT CCTCTCATAC CTAGTTATAA GCTTTTTGAT AGTTATTAAT 
ATGTACATTG CTGTCATTCT CGAGAACTAT AGTCAGGCCA CCGAGGACGT GCAAGAGGGT 
CTAACCGACG ACGACTACGA CATGTACTAT GAGATCTGGC AGCAATTCGA TCCGGAGGGC 
ACCCAGTACA TACGCTATGA TCAGCTGTCC GAATTCCTGG ACGTACTGGA GCCCCCGCTG 
CAGATCCACA AACCGAACAA GTACAAGATC ATATCGATGG ACATACCCAT CTGTCGCGGT 
GACCTCATGT ACTGCGTCGA CATCCTCGAC GCCCTTACGA AAGACTTCTT TGCGCGGAAG 
GGCAATCCGA TAGAGGAGAC GGGTGAGATT GGTGAGATAG CGGCCCGCCC GGATACGGAG 
GGCTACGAGC CCGTCTCATC AACGCTGTGG CGTCAGCGTG AGGAGTACTG CGCCCGGCTA 
ATCCAGCACG CCTGGCGAAA GCACAAGGCG CGCGGCGAGG GAGGTGGGTC CTTTGAGCCG 
GATACGGATC ATGGCGATGG CGGTGATCCG GATGCCGGGG ACCCGGCGCC CGATGAAGCA 
ACGGACGGCG ATGCGCCCGC TGGTGGAGAT GGTAGTGTTA ACGGTACTGC AGAAGGAGCT 
GCCGATGCCG ATGAGAGTAA TGTAAATAGT CCGGGTGAGG ATGCAGCGGC GGCGGCAGCA 
GCAGCAGCAG CAGCGGCGGC GGCGGGCACG ACGACGGCGG GAAGTCCCGG AGCGGGTAGC 
GCCGGGCGAC AGACCGCCGT TCTCGTGGAG AGCGACGGGT TCGTGACGAA GAACGGCCAC 
AAGGTGGTCA TCCACTCGCG ATCGCCGAGC ATCACGTCGC GCACGGCGGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201706.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188635.1 Drosophila melanogaster paralytic (para), transcript variant AN, mRNA.

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CloneID	OFa01823
Clone ID Related Accession (Same CDS sequence)	NM_001201706.1
Accession Version	NM_001201706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AN
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGCAAA 
GGCGTTTGTC GTTGTATATC TGCAGAGCAT GGTGACAACG AACTGGAGCT GGGCCACGAC 
GAGATCCTCG CCGACGGCCT CATCAAGAAG GGGATCAAGG AGCAGACGCA ACTGGAGGTG 
GCCATCGGGG ATGGCATGGA ATTCACGATA CACGGCGACA TGAAGAACAA CAAGCCGAAG 
AAATCCAAAT ATCTAAATAA CGCAACGATG ATTGGCAACT CAATTAACCA CCAAGACAAT 
AGACTGGAAC ACGAGCTAAA CCATAGAGGT TTGTCCTTAC AGGACGACGA CACTGCCAGC 
ATTAACTCAT ATGGTAGCCA TAAGAATCGA CCATTCAAGG ACGAGAGCCA CAAGGGCAGC 
GCCGAGACGA TGGAGGGCGA GGAGAAGCGC GACGCCAGCA AGGAGGATTT AGGTCTCGAC 
GAGGAACTGG ACGAGGAGGG CGAATGCGAG GAGGGCCCGC TCGACGGTGA TATCATTATT 
CATGCACACG ACGAGGATAT ACTCGATGAA TATCCAGCTG ATTGCTGCCC CGATTCGTAC 
TATAAGAAAT TTCCGATCTT AGCCGGTGAC GATGACTCGC CGTTCTGGCA AGGATGGGGC 
AATTTACGAC TGAAAACTTT TCAATTAATT GAAAATAAAT ATTTTGAAAC AGCTGTTATC 
ACTATGATTT TAATGAGTAG CTTAGCTTTG GCATTAGAAG ATGTACATCT GCCACAAAGA 
CCCATACTGC AGGATATTTT ATACTATATG GACAGAATAT TTACGGTTAT ATTCTTCTTG 
GAAATGTTAA TCAAGTGGTT GGCGCTCGGC TTCAAAGTGT ACTTCACCAA CGCGTGGTGT 
TGGCTCGATT TCGTGATTGT CATGGTATCG CTTATCAACT TCGTTGCTTC ACTTGTTGGA 
GCTGGTGGTA TTCAAGCCTT CAAGACTATG CGAACGTTAA GAGCACTGAG ACCACTACGT 
GCCATGTCCC GTATGCAGGG CATGAGGGTC GTCGTTAATG CGCTGGTACA AGCTATACCG 
TCCATCTTCA ATGTGCTATT GGTGTGTCTA ATATTTTGGC TAATTTTTGC CATAATGGGT 
GTACAGCTTT TTGCTGGAAA ATATTTTAAG TGCGAGGACA TGAATGGCAC GAAGCTCAGC 
CACGAGATCA TACCAAATCG CAATGCCTGC GAGAGCGAGA ACTACACGTG GGTGAATTCA 
GCAATGAATT TCGATCATGT AGGTAACGCG TATCTGTGCC TTTTCCAAGT GGCCACCTTC 
AAAGGCTGGA TACAAATCAT GAACGATGCT ATCGATTCAC GAGAGGTGGA CAAGCAACCA 
ATTCGTGAAA CGAACATCTA CATGTATTTA TATTTCGTAT TCTTCATCAT ATTTGGATCA 
TTTTTCACAC TCAATCTGTT CATTGGTGTT ATCATTGATA ATTTTAATGA GCAAAAGAAA 
AAAGCAGGTG GATCATTAGA AATGTTCATG ACAGAAGATC AGAAAAAGTA CTATAATGCT 
ATGAAAAAGA TGGGCTCTAA AAAACCATTA AAAGCCATTC CAAGACCAAG GTGGCGACCA 
CAAGCAATAG TCTTTGAAAT AGTAACCGAT AAGAAATTCG ATATAATCAT TATGTTATTC 
ATTGGTCTGA ACATGTTCAC CATGACCCTC GATCGTTACG ATGCGTCGGA CACGTATAAC 
GCGGTCCTAG ACTATCTCAA TGCGATATTC GTAGTTATTT TCAGTTCCGA ATGTCTATTA 
AAAATATTCG CTTTACGATA TCACTATTTT ATTGAGCCAT GGAATTTATT TGATGTAGTA 
GTTGTCATTT TATCCATCTT AGGTCTTGTA CTTAGCGATA TTATCGAGAA GTACTTCGTG 
TCGCCGACCC TGCTCCGAGT GGTGCGTGTG GCGAAAGTGG GCCGTGTCCT TCGACTGGTG 
AAGGGAGCCA AGGGCATTCG GACACTGCTC TTCGCGTTGG CCATGTCGCT GCCGGCCCTG 
TTCAACATCT GCCTGCTGCT GTTCCTGGTC ATGTTCATCT TTGCCATTTT CGGCATGTCG 
TTCTTCATGC ACGTGAAGGA GAAGAGCGGC ATCAACGACG TCTACAACTT CAAGACCTTT 
GGCCAGAGCA TGATCCTGCT CTTTCAGATG TCGACGTCAG CCGGTTGGGA TGGTGTACTG 
GACGCCATTA TCAATGAGGA AGCATGCGAT CCACCCGACA ACGACAAAGG CTATCCGGGC 
AATTGTGGTT CAGCGACCGT TGGAATAACG TTTCTCCTCT CATACCTAGT TATAAGCTTT 
TTGATAGTTA TTAATATGTA CATTGCTGTC ATTCTCGAGA ACTATAGTCA GGCCACCGAG 
GACGTGCAAG AGGGTCTAAC CGACGACGAC TACGACATGT ACTATGAGAT CTGGCAGCAA 
TTCGATCCGG AGGGCACCCA GTACATACGC TATGATCAGC TGTCCGAATT CCTGGACGTA 
CTGGAGCCCC CGCTGCAGAT CCACAAACCG AACAAGTACA AGATCATATC GATGGACATA 
CCCATCTGTC GCGGTGACCT CATGTACTGC GTCGACATCC TCGACGCCCT TACGAAAGAC 
TTCTTTGCGC GGAAGGGCAA TCCGATAGAG GAGACGGGTG AGATTGGTGA GATAGCGGCC 
CGCCCGGATA CGGAGGGCTA CGAGCCCGTC TCATCAACGC TGTGGCGTCA GCGTGAGGAG 
TACTGCGCCC GGCTAATCCA GCACGCCTGG CGAAAGCACA AGGCGCGCGG CGAGGGAGGT 
GGGTCCTTTG AGCCGGATAC GGATCATGGC GATGGCGGTG ATCCGGATGC CGGGGACCCG 
GCGCCCGATG AAGCAACGGA CGGCGATGCG CCCGCTGGTG GAGATGGTAG TGTTAACGGT 
ACTGCAGAAG GAGCTGCCGA TGCCGATGAG AGTAATGTAA ATAGTCCGGG TGAGGATGCA 
GCGGCGGCGG CAGCAGCAGC AGCAGCAGCG GCGGCGGCGG GCACGACGAC GGCGGGAAGT 
CCCGGAGCGG GTAGCGCCGG GCGACAGACC GCCGTTCTCG TGGAGAGCGA CGGGTTCGTG 
ACGAAGAACG GCCACAAGGT GGTCATCCAC TCGCGATCGC CGAGCATCAC GTCGCGCACG 
GCGGATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188635.1
CDS	269..6700
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATMIGNSINHQDNRLEHELNHRGLSLQDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201706.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AN, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201706.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGCAAA 
GGCGTTTGTC GTTGTATATC TGCAGAGCAT GGTGACAACG AACTGGAGCT GGGCCACGAC 
GAGATCCTCG CCGACGGCCT CATCAAGAAG GGGATCAAGG AGCAGACGCA ACTGGAGGTG 
GCCATCGGGG ATGGCATGGA ATTCACGATA CACGGCGACA TGAAGAACAA CAAGCCGAAG 
AAATCCAAAT ATCTAAATAA CGCAACGATG ATTGGCAACT CAATTAACCA CCAAGACAAT 
AGACTGGAAC ACGAGCTAAA CCATAGAGGT TTGTCCTTAC AGGACGACGA CACTGCCAGC 
ATTAACTCAT ATGGTAGCCA TAAGAATCGA CCATTCAAGG ACGAGAGCCA CAAGGGCAGC 
GCCGAGACGA TGGAGGGCGA GGAGAAGCGC GACGCCAGCA AGGAGGATTT AGGTCTCGAC 
GAGGAACTGG ACGAGGAGGG CGAATGCGAG GAGGGCCCGC TCGACGGTGA TATCATTATT 
CATGCACACG ACGAGGATAT ACTCGATGAA TATCCAGCTG ATTGCTGCCC CGATTCGTAC 
TATAAGAAAT TTCCGATCTT AGCCGGTGAC GATGACTCGC CGTTCTGGCA AGGATGGGGC 
AATTTACGAC TGAAAACTTT TCAATTAATT GAAAATAAAT ATTTTGAAAC AGCTGTTATC 
ACTATGATTT TAATGAGTAG CTTAGCTTTG GCATTAGAAG ATGTACATCT GCCACAAAGA 
CCCATACTGC AGGATATTTT ATACTATATG GACAGAATAT TTACGGTTAT ATTCTTCTTG 
GAAATGTTAA TCAAGTGGTT GGCGCTCGGC TTCAAAGTGT ACTTCACCAA CGCGTGGTGT 
TGGCTCGATT TCGTGATTGT CATGGTATCG CTTATCAACT TCGTTGCTTC ACTTGTTGGA 
GCTGGTGGTA TTCAAGCCTT CAAGACTATG CGAACGTTAA GAGCACTGAG ACCACTACGT 
GCCATGTCCC GTATGCAGGG CATGAGGGTC GTCGTTAATG CGCTGGTACA AGCTATACCG 
TCCATCTTCA ATGTGCTATT GGTGTGTCTA ATATTTTGGC TAATTTTTGC CATAATGGGT 
GTACAGCTTT TTGCTGGAAA ATATTTTAAG TGCGAGGACA TGAATGGCAC GAAGCTCAGC 
CACGAGATCA TACCAAATCG CAATGCCTGC GAGAGCGAGA ACTACACGTG GGTGAATTCA 
GCAATGAATT TCGATCATGT AGGTAACGCG TATCTGTGCC TTTTCCAAGT GGCCACCTTC 
AAAGGCTGGA TACAAATCAT GAACGATGCT ATCGATTCAC GAGAGGTGGA CAAGCAACCA 
ATTCGTGAAA CGAACATCTA CATGTATTTA TATTTCGTAT TCTTCATCAT ATTTGGATCA 
TTTTTCACAC TCAATCTGTT CATTGGTGTT ATCATTGATA ATTTTAATGA GCAAAAGAAA 
AAAGCAGGTG GATCATTAGA AATGTTCATG ACAGAAGATC AGAAAAAGTA CTATAATGCT 
ATGAAAAAGA TGGGCTCTAA AAAACCATTA AAAGCCATTC CAAGACCAAG GTGGCGACCA 
CAAGCAATAG TCTTTGAAAT AGTAACCGAT AAGAAATTCG ATATAATCAT TATGTTATTC 
ATTGGTCTGA ACATGTTCAC CATGACCCTC GATCGTTACG ATGCGTCGGA CACGTATAAC 
GCGGTCCTAG ACTATCTCAA TGCGATATTC GTAGTTATTT TCAGTTCCGA ATGTCTATTA 
AAAATATTCG CTTTACGATA TCACTATTTT ATTGAGCCAT GGAATTTATT TGATGTAGTA 
GTTGTCATTT TATCCATCTT AGGTCTTGTA CTTAGCGATA TTATCGAGAA GTACTTCGTG 
TCGCCGACCC TGCTCCGAGT GGTGCGTGTG GCGAAAGTGG GCCGTGTCCT TCGACTGGTG 
AAGGGAGCCA AGGGCATTCG GACACTGCTC TTCGCGTTGG CCATGTCGCT GCCGGCCCTG 
TTCAACATCT GCCTGCTGCT GTTCCTGGTC ATGTTCATCT TTGCCATTTT CGGCATGTCG 
TTCTTCATGC ACGTGAAGGA GAAGAGCGGC ATCAACGACG TCTACAACTT CAAGACCTTT 
GGCCAGAGCA TGATCCTGCT CTTTCAGATG TCGACGTCAG CCGGTTGGGA TGGTGTACTG 
GACGCCATTA TCAATGAGGA AGCATGCGAT CCACCCGACA ACGACAAAGG CTATCCGGGC 
AATTGTGGTT CAGCGACCGT TGGAATAACG TTTCTCCTCT CATACCTAGT TATAAGCTTT 
TTGATAGTTA TTAATATGTA CATTGCTGTC ATTCTCGAGA ACTATAGTCA GGCCACCGAG 
GACGTGCAAG AGGGTCTAAC CGACGACGAC TACGACATGT ACTATGAGAT CTGGCAGCAA 
TTCGATCCGG AGGGCACCCA GTACATACGC TATGATCAGC TGTCCGAATT CCTGGACGTA 
CTGGAGCCCC CGCTGCAGAT CCACAAACCG AACAAGTACA AGATCATATC GATGGACATA 
CCCATCTGTC GCGGTGACCT CATGTACTGC GTCGACATCC TCGACGCCCT TACGAAAGAC 
TTCTTTGCGC GGAAGGGCAA TCCGATAGAG GAGACGGGTG AGATTGGTGA GATAGCGGCC 
CGCCCGGATA CGGAGGGCTA CGAGCCCGTC TCATCAACGC TGTGGCGTCA GCGTGAGGAG 
TACTGCGCCC GGCTAATCCA GCACGCCTGG CGAAAGCACA AGGCGCGCGG CGAGGGAGGT 
GGGTCCTTTG AGCCGGATAC GGATCATGGC GATGGCGGTG ATCCGGATGC CGGGGACCCG 
GCGCCCGATG AAGCAACGGA CGGCGATGCG CCCGCTGGTG GAGATGGTAG TGTTAACGGT 
ACTGCAGAAG GAGCTGCCGA TGCCGATGAG AGTAATGTAA ATAGTCCGGG TGAGGATGCA 
GCGGCGGCGG CAGCAGCAGC AGCAGCAGCG GCGGCGGCGG GCACGACGAC GGCGGGAAGT 
CCCGGAGCGG GTAGCGCCGG GCGACAGACC GCCGTTCTCG TGGAGAGCGA CGGGTTCGTG 
ACGAAGAACG GCCACAAGGT GGTCATCCAC TCGCGATCGC CGAGCATCAC GTCGCGCACG 
GCGGATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201682.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188611.1 Drosophila melanogaster paralytic (para), transcript variant P, mRNA.

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CloneID	OFa01824
Clone ID Related Accession (Same CDS sequence)	NM_001201682.1
Accession Version	NM_001201682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform P
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGAGCATG GTGACAACGA ACTGGAGCTG 
GGCCACGACG AGATCCTCGC CGACGGCCTC ATCAAGAAGG GGATCAAGGA GCAGACGCAA 
CTGGAGGTGG CCATCGGGGA TGGCATGGAA TTCACGATAC ACGGCGACAT GAAGAACAAC 
AAGCCGAAGA AATCCAAATA TCTAAATAAC GCAACGGACG ACGACACTGC CAGCATTAAC 
TCATATGGTA GCCATAAGAA TCGACCATTC AAGGACGAGA GCCACAAGGG CAGCGCCGAG 
ACGATGGAGG GCGAGGAGAA GCGCGACGCC AGCAAGGAGG ATTTAGGTCT CGACGAGGAA 
CTGGACGAGG AGGGCGAATG CGAGGAGGGC CCGCTCGACG GTGATATCAT TATTCATGCA 
CACGACGAGG ATATACTCGA TGAATATCCA GCTGATTGCT GCCCCGATTC GTACTATAAG 
AAATTTCCGA TCTTAGCCGG TGACGATGAC TCGCCGTTCT GGCAAGGATG GGGCAATTTA 
CGACTGAAAA CTTTTCAATT AATTGAAAAT AAATATTTTG AAACAGCTGT TATCACTATG 
ATTTTAATGA GTAGCTTAGC TTTGGCATTA GAAGATGTAC ATCTGCCACA AAGACCCATA 
CTGCAGGATA TTTTATACTA TATGGACAGA ATATTTACGG TTATATTCTT CTTGGAAATG 
TTAATCAAGT GGTTGGCGCT CGGCTTCAAA GTGTACTTCA CCAACGCGTG GTGTTGGCTC 
GATTTCGTGA TTGTCATGGT ATCGCTTATC AACTTCGTTG CTTCACTTGT TGGAGCTGGT 
GGTATTCAAG CCTTCAAGAC TATGCGAACG TTAAGAGCAC TGAGACCACT ACGTGCCATG 
TCCCGTATGC AGGGCATGAG GGTCGTCGTT AATGCGCTGG TACAAGCTAT ACCGTCCATC 
TTCAATGTGC TATTGGTGTG TCTAATATTT TGGCTAATTT TTGCCATAAT GGGTGTACAG 
CTTTTTGCTG GAAAATATTT TAAGTGCGAG GACATGAATG GCACGAAGCT CAGCCACGAG 
ATCATACCAA ATCGCAATGC CTGCGAGAGC GAGAACTACA CGTGGGTGAA TTCAGCAATG 
AATTTCGATC ATGTAGGTAA CGCGTATCTG TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC 
TGGATACAAA TCATGAACGA TGCTATCGAT TCACGAGAGG TGGACAAGCA ACCAATTCGT 
GAAACGAACA TCTACATGTA TTTATATTTC GTATTCTTCA TCATATTTGG ATCATTTTTC 
ACACTCAATC TGTTCATTGG TGTTATCATT GATAATTTTA ATGAGCAAAA GAAAAAAGCA 
GGTGGATCAT TAGAAATGTT CATGACAGAA GATCAGAAAA AGTACTATAA TGCTATGAAA 
AAGATGGGCT CTAAAAAACC ATTAAAAGCC ATTCCAAGAC CAAGGTGGCG ACCACAAGCA 
ATAGTCTTTG AAATAGTAAC CGATAAGAAA TTCGATATAA TCATTATGTT ATTCATTGGT 
CTGAACATGT TCACCATGAC CCTCGATCGT TACGATGCGT CGGACACGTA TAACGCGGTC 
CTAGACTATC TCAATGCGAT ATTCGTAGTT ATTTTCAGTT CCGAATGTCT ATTAAAAATA 
TTCGCTTTAC GATATCACTA TTTTATTGAG CCATGGAATT TATTTGATGT AGTAGTTGTC 
ATTTTATCCA TCTTAGGTCT TGTACTTAGC GATATTATCG AGAAGTACTT CGTGTCGCCG 
ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA GTGGGCCGTG TCCTTCGACT GGTGAAGGGA 
GCCAAGGGCA TTCGGACACT GCTCTTCGCG TTGGCCATGT CGCTGCCGGC CCTGTTCAAC 
ATCTGCCTGC TGCTGTTCCT GGTCATGTTC ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC 
ATGCACGTGA AGGAGAAGAG CGGCATCAAC GACGTCTACA ACTTCAAGAC CTTTGGCCAG 
AGCATGATCC TGCTCTTTCA GATGTCGACG TCAGCCGGTT GGGATGGTGT ACTGGACGCC 
ATTATCAATG AGGAAGCATG CGATCCACCC GACAACGACA AAGGCTATCC GGGCAATTGT 
GGTTCAGCGA CCGTTGGAAT AACGTTTCTC CTCTCATACC TAGTTATAAG CTTTTTGATA 
GTTATTAATA TGTACATTGC TGTCATTCTC GAGAACTATA GTCAGGCCAC CGAGGACGTG 
CAAGAGGGTC TAACCGACGA CGACTACGAC ATGTACTATG AGATCTGGCA GCAATTCGAT 
CCGGAGGGCA CCCAGTACAT ACGCTATGAT CAGCTGTCCG AATTCCTGGA CGTACTGGAG 
CCCCCGCTGC AGATCCACAA ACCGAACAAG TACAAGATCA TATCGATGGA CATACCCATC 
TGTCGCGGTG ACCTCATGTA CTGCGTCGAC ATCCTCGACG CCCTTACGAA AGACTTCTTT 
GCGCGGAAGG GCAATCCGAT AGAGGAGACG GGTGAGATTG GTGAGATAGC GGCCCGCCCG 
GATACGGAGG GCTACGAGCC CGTCTCATCA ACGCTGTGGC GTCAGCGTGA GGAGTACTGC 
GCCCGGCTAA TCCAGCACGC CTGGCGAAAG CACAAGGCGC GCGGCGAGGG AGGTGGGTCC 
TTTGAGCCGG ATACGGATCA TGGCGATGGC GGTGATCCGG ATGCCGGGGA CCCGGCGCCC 
GATGAAGCAA CGGACGGCGA TGCGCCCGCT GGTGGAGATG GTAGTGTTAA CGGTACTGCA 
GAAGGAGCTG CCGATGCCGA TGAGAGTAAT GTAAATAGTC CGGGTGAGGA TGCAGCGGCG 
GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG GCGGGCACGA CGACGGCGGG AAGTCCCGGA 
GCGGGTAGCG CCGGGCGACA GACCGCCGTT CTCGTGGAGA GCGACGGGTT CGTGACGAAG 
AACGGCCACA AGGTGGTCAT CCACTCGCGA TCGCCGAGCA TCACGTCGCG CACGGCGGAT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188611.1
CDS	269..6634
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201682.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant P, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201682.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGAGCATG GTGACAACGA ACTGGAGCTG 
GGCCACGACG AGATCCTCGC CGACGGCCTC ATCAAGAAGG GGATCAAGGA GCAGACGCAA 
CTGGAGGTGG CCATCGGGGA TGGCATGGAA TTCACGATAC ACGGCGACAT GAAGAACAAC 
AAGCCGAAGA AATCCAAATA TCTAAATAAC GCAACGGACG ACGACACTGC CAGCATTAAC 
TCATATGGTA GCCATAAGAA TCGACCATTC AAGGACGAGA GCCACAAGGG CAGCGCCGAG 
ACGATGGAGG GCGAGGAGAA GCGCGACGCC AGCAAGGAGG ATTTAGGTCT CGACGAGGAA 
CTGGACGAGG AGGGCGAATG CGAGGAGGGC CCGCTCGACG GTGATATCAT TATTCATGCA 
CACGACGAGG ATATACTCGA TGAATATCCA GCTGATTGCT GCCCCGATTC GTACTATAAG 
AAATTTCCGA TCTTAGCCGG TGACGATGAC TCGCCGTTCT GGCAAGGATG GGGCAATTTA 
CGACTGAAAA CTTTTCAATT AATTGAAAAT AAATATTTTG AAACAGCTGT TATCACTATG 
ATTTTAATGA GTAGCTTAGC TTTGGCATTA GAAGATGTAC ATCTGCCACA AAGACCCATA 
CTGCAGGATA TTTTATACTA TATGGACAGA ATATTTACGG TTATATTCTT CTTGGAAATG 
TTAATCAAGT GGTTGGCGCT CGGCTTCAAA GTGTACTTCA CCAACGCGTG GTGTTGGCTC 
GATTTCGTGA TTGTCATGGT ATCGCTTATC AACTTCGTTG CTTCACTTGT TGGAGCTGGT 
GGTATTCAAG CCTTCAAGAC TATGCGAACG TTAAGAGCAC TGAGACCACT ACGTGCCATG 
TCCCGTATGC AGGGCATGAG GGTCGTCGTT AATGCGCTGG TACAAGCTAT ACCGTCCATC 
TTCAATGTGC TATTGGTGTG TCTAATATTT TGGCTAATTT TTGCCATAAT GGGTGTACAG 
CTTTTTGCTG GAAAATATTT TAAGTGCGAG GACATGAATG GCACGAAGCT CAGCCACGAG 
ATCATACCAA ATCGCAATGC CTGCGAGAGC GAGAACTACA CGTGGGTGAA TTCAGCAATG 
AATTTCGATC ATGTAGGTAA CGCGTATCTG TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC 
TGGATACAAA TCATGAACGA TGCTATCGAT TCACGAGAGG TGGACAAGCA ACCAATTCGT 
GAAACGAACA TCTACATGTA TTTATATTTC GTATTCTTCA TCATATTTGG ATCATTTTTC 
ACACTCAATC TGTTCATTGG TGTTATCATT GATAATTTTA ATGAGCAAAA GAAAAAAGCA 
GGTGGATCAT TAGAAATGTT CATGACAGAA GATCAGAAAA AGTACTATAA TGCTATGAAA 
AAGATGGGCT CTAAAAAACC ATTAAAAGCC ATTCCAAGAC CAAGGTGGCG ACCACAAGCA 
ATAGTCTTTG AAATAGTAAC CGATAAGAAA TTCGATATAA TCATTATGTT ATTCATTGGT 
CTGAACATGT TCACCATGAC CCTCGATCGT TACGATGCGT CGGACACGTA TAACGCGGTC 
CTAGACTATC TCAATGCGAT ATTCGTAGTT ATTTTCAGTT CCGAATGTCT ATTAAAAATA 
TTCGCTTTAC GATATCACTA TTTTATTGAG CCATGGAATT TATTTGATGT AGTAGTTGTC 
ATTTTATCCA TCTTAGGTCT TGTACTTAGC GATATTATCG AGAAGTACTT CGTGTCGCCG 
ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA GTGGGCCGTG TCCTTCGACT GGTGAAGGGA 
GCCAAGGGCA TTCGGACACT GCTCTTCGCG TTGGCCATGT CGCTGCCGGC CCTGTTCAAC 
ATCTGCCTGC TGCTGTTCCT GGTCATGTTC ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC 
ATGCACGTGA AGGAGAAGAG CGGCATCAAC GACGTCTACA ACTTCAAGAC CTTTGGCCAG 
AGCATGATCC TGCTCTTTCA GATGTCGACG TCAGCCGGTT GGGATGGTGT ACTGGACGCC 
ATTATCAATG AGGAAGCATG CGATCCACCC GACAACGACA AAGGCTATCC GGGCAATTGT 
GGTTCAGCGA CCGTTGGAAT AACGTTTCTC CTCTCATACC TAGTTATAAG CTTTTTGATA 
GTTATTAATA TGTACATTGC TGTCATTCTC GAGAACTATA GTCAGGCCAC CGAGGACGTG 
CAAGAGGGTC TAACCGACGA CGACTACGAC ATGTACTATG AGATCTGGCA GCAATTCGAT 
CCGGAGGGCA CCCAGTACAT ACGCTATGAT CAGCTGTCCG AATTCCTGGA CGTACTGGAG 
CCCCCGCTGC AGATCCACAA ACCGAACAAG TACAAGATCA TATCGATGGA CATACCCATC 
TGTCGCGGTG ACCTCATGTA CTGCGTCGAC ATCCTCGACG CCCTTACGAA AGACTTCTTT 
GCGCGGAAGG GCAATCCGAT AGAGGAGACG GGTGAGATTG GTGAGATAGC GGCCCGCCCG 
GATACGGAGG GCTACGAGCC CGTCTCATCA ACGCTGTGGC GTCAGCGTGA GGAGTACTGC 
GCCCGGCTAA TCCAGCACGC CTGGCGAAAG CACAAGGCGC GCGGCGAGGG AGGTGGGTCC 
TTTGAGCCGG ATACGGATCA TGGCGATGGC GGTGATCCGG ATGCCGGGGA CCCGGCGCCC 
GATGAAGCAA CGGACGGCGA TGCGCCCGCT GGTGGAGATG GTAGTGTTAA CGGTACTGCA 
GAAGGAGCTG CCGATGCCGA TGAGAGTAAT GTAAATAGTC CGGGTGAGGA TGCAGCGGCG 
GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG GCGGGCACGA CGACGGCGGG AAGTCCCGGA 
GCGGGTAGCG CCGGGCGACA GACCGCCGTT CTCGTGGAGA GCGACGGGTT CGTGACGAAG 
AACGGCCACA AGGTGGTCAT CCACTCGCGA TCGCCGAGCA TCACGTCGCG CACGGCGGAT 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001272690.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259619.1 Drosophila melanogaster paralytic (para), transcript variant BF, mRNA.

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CloneID	OFa01825
Clone ID Related Accession (Same CDS sequence)	NM_001272690.1
Accession Version	NM_001272690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform BF
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGGCAAAG GCGTTTGTCG TTGTATATCT 
GCAGAGCATG GTGACAACGA ACTGGAGCTG GGCCACGACG AGATCCTCGC CGACGGCCTC 
ATCAAGAAGG GGATCAAGGA GCAGACGCAA CTGGAGGTGG CCATCGGGGA TGGCATGGAA 
TTCACGATAC ACGGCGACAT GAAGAACAAC AAGCCGAAGA AATCCAAATA TCTAAATAAC 
GCAACGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGGT ATCGCTTATC 
AACTTCGTTG CTTCACTTGT TGGAGCTGGT GGTATTCAAG CCTTCAAGAC TATGCGAACG 
TTAAGAGCAC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGCATGAG GGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGAGCCA GGCCTCGCCC CCCCCTCCAA 
GATGCACGCG AGTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259619.1
CDS	269..6706
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV*ARPRPPLQDAREY

Target ORF information:

RefSeq Version	NM_001272690.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant BF, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272690.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGGCAAAG GCGTTTGTCG TTGTATATCT 
GCAGAGCATG GTGACAACGA ACTGGAGCTG GGCCACGACG AGATCCTCGC CGACGGCCTC 
ATCAAGAAGG GGATCAAGGA GCAGACGCAA CTGGAGGTGG CCATCGGGGA TGGCATGGAA 
TTCACGATAC ACGGCGACAT GAAGAACAAC AAGCCGAAGA AATCCAAATA TCTAAATAAC 
GCAACGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGGT ATCGCTTATC 
AACTTCGTTG CTTCACTTGT TGGAGCTGGT GGTATTCAAG CCTTCAAGAC TATGCGAACG 
TTAAGAGCAC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGCATGAG GGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGAGCCA GGCCTCGCCC CCCCCTCCAA 
GATGCACGCG AGTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201672.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188601.1 Drosophila melanogaster paralytic (para), transcript variant F, mRNA.

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CloneID	OFa01826
Clone ID Related Accession (Same CDS sequence)	NM_001201672.1
Accession Version	NM_001201672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTTTT CAAGCTGGCC 
AAATCGTGGC CGACGCTGAA TTTGCTCATA TCGATTATGG GCCGCACTGT CGGCGCCCTC 
GGCAATCTGA CCTTCGTCCT GTGCATTATT ATCTTCATAT TCGCGGTCAT GGGCATGCAG 
CTGTTTGGCA AGAACTACAC AGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGAGCAT 
GGTGACAACG AACTGGAGCT GGGCCACGAC GAGATCCTCG CCGACGGCCT CATCAAGAAG 
GGGATCAAGG AGCAGACGCA ACTGGAGGTG GCCATCGGGG ATGGCATGGA ATTCACGATA 
CACGGCGACA TGAAGAACAA CAAGCCGAAG AAATCCAAAT ATCTAAATAA CGCAACGGAC 
GACGACACTG CCAGCATTAA CTCATATGGT AGCCATAAGA ATCGACCATT CAAGGACGAG 
AGCCACAAGG GCAGCGCCGA GACGATGGAG GGCGAGGAGA AGCGCGACGC CAGCAAGGAG 
GATTTAGGTC TCGACGAGGA ACTGGACGAG GAGGGCGAAT GCGAGGAGGG CCCGCTCGAC 
GGTGATATCA TTATTCATGC ACACGACGAG GATATACTCG ATGAATATCC AGCTGATTGC 
TGCCCCGATT CGTACTATAA GAAATTTCCG ATCTTAGCCG GTGACGATGA CTCGCCGTTC 
TGGCAAGGAT GGGGCAATTT ACGACTGAAA ACTTTTCAAT TAATTGAAAA TAAATATTTT 
GAAACAGCTG TTATCACTAT GATTTTAATG AGTAGCTTAG CTTTGGCATT AGAAGATGTA 
CATCTGCCAC AAAGACCCAT ACTGCAGGAT ATTTTATACT ATATGGACAG AATATTTACG 
GTTATATTCT TCTTGGAAAT GTTAATCAAG TGGTTGGCGC TCGGCTTCAA AGTGTACTTC 
ACCAACGCGT GGTGTTGGCT CGATTTCGTG ATTGTCATGG TATCGCTTAT CAACTTCGTT 
GCTTCACTTG TTGGAGCTGG TGGTATTCAA GCCTTCAAGA CTATGCGAAC GTTAAGAGCA 
CTGAGACCAC TACGTGCCAT GTCCCGTATG CAGGGCATGA GGGTCGTCGT TAATGCGCTG 
GTACAAGCTA TACCGTCCAT CTTCAATGTG CTATTGGTGT GTCTAATATT TTGGCTAATT 
TTTGCCATAA TGGGTGTACA GCTTTTTGCT GGAAAATATT TTAAGTGCGA GGACATGAAT 
GGCACGAAGC TCAGCCACGA GATCATACCA AATCGCAATG CCTGCGAGAG CGAGAACTAC 
ACGTGGGTGA ATTCAGCAAT GAATTTCGAT CATGTAGGTA ACGCGTATCT GTGCCTTTTC 
CAAGTGGCCA CCTTCAAAGG CTGGATACAA ATCATGAACG ATGCTATCGA TTCACGAGAG 
GTGGACAAGC AACCAATTCG TGAAACGAAC ATCTACATGT ATTTATATTT CGTATTCTTC 
ATCATATTTG GATCATTTTT CACACTCAAT CTGTTCATTG GTGTTATCAT TGATAATTTT 
AATGAGCAAA AGAAAAAAGC AGGTGGATCA TTAGAAATGT TCATGACAGA AGATCAGAAA 
AAGTACTATA ATGCTATGAA AAAGATGGGC TCTAAAAAAC CATTAAAAGC CATTCCAAGA 
CCAAGGTGGC GACCACAAGC AATAGTCTTT GAAATAGTAA CCGATAAGAA ATTCGATATA 
ATCATTATGT TATTCATTGG TCTGAACATG TTCACCATGA CCCTCGATCG TTACGATGCG 
TCGGACACGT ATAACGCGGT CCTAGACTAT CTCAATGCGA TATTCGTAGT TATTTTCAGT 
TCCGAATGTC TATTAAAAAT ATTCGCTTTA CGATATCACT ATTTTATTGA GCCATGGAAT 
TTATTTGATG TAGTAGTTGT CATTTTATCC ATCTTAGGTC TTGTACTTAG CGATATTATC 
GAGAAGTACT TCGTGTCGCC GACCCTGCTC CGAGTGGTGC GTGTGGCGAA AGTGGGCCGT 
GTCCTTCGAC TGGTGAAGGG AGCCAAGGGC ATTCGGACAC TGCTCTTCGC GTTGGCCATG 
TCGCTGCCGG CCCTGTTCAA CATCTGCCTG CTGCTGTTCC TGGTCATGTT CATCTTTGCC 
ATTTTCGGCA TGTCGTTCTT CATGCACGTG AAGGAGAAGA GCGGCATCAA CGACGTCTAC 
AACTTCAAGA CCTTTGGCCA GAGCATGATC CTGCTCTTTC AGATGTCGAC GTCAGCCGGT 
TGGGATGGTG TACTGGACGC CATTATCAAT GAGGAAGCAT GCGATCCACC CGACAACGAC 
AAAGGCTATC CGGGCAATTG TGGTTCAGCG ACCGTTGGAA TAACGTTTCT CCTCTCATAC 
CTAGTTATAA GCTTTTTGAT AGTTATTAAT ATGTACATTG CTGTCATTCT CGAGAACTAT 
AGTCAGGCCA CCGAGGACGT GCAAGAGGGT CTAACCGACG ACGACTACGA CATGTACTAT 
GAGATCTGGC AGCAATTCGA TCCGGAGGGC ACCCAGTACA TACGCTATGA TCAGCTGTCC 
GAATTCCTGG ACGTACTGGA GCCCCCGCTG CAGATCCACA AACCGAACAA GTACAAGATC 
ATATCGATGG ACATACCCAT CTGTCGCGGT GACCTCATGT ACTGCGTCGA CATCCTCGAC 
GCCCTTACGA AAGACTTCTT TGCGCGGAAG GGCAATCCGA TAGAGGAGAC GGGTGAGATT 
GGTGAGATAG CGGCCCGCCC GGATACGGAG GGCTACGAGC CCGTCTCATC AACGCTGTGG 
CGTCAGCGTG AGGAGTACTG CGCCCGGCTA ATCCAGCACG CCTGGCGAAA GCACAAGGCG 
CGCGGCGAGG GAGGTGGGTC CTTTGAGCCG GATACGGATC ATGGCGATGG CGGTGATCCG 
GATGCCGGGG ACCCGGCGCC CGATGAAGCA ACGGACGGCG ATGCGCCCGC TGGTGGAGAT 
GGTAGTGTTA ACGGTACTGC AGAAGGAGCT GCCGATGCCG ATGAGAGTAA TGTAAATAGT 
CCGGGTGAGG ATGCAGCGGC GGCGGCAGCA GCAGCAGCAG CAGCGGCGGC GGCGGGCACG 
ACGACGGCGG GAAGTCCCGG AGCGGGTAGC GCCGGGCGAC AGACCGCCGT TCTCGTGGAG 
AGCGACGGGT TCGTGACGAA GAACGGCCAC AAGGTGGTCA TCCACTCGCG ATCGCCGAGC 
ATCACGTCGC GCACGGCGGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188601.1
CDS	269..6595
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201672.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201672.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTTTT CAAGCTGGCC 
AAATCGTGGC CGACGCTGAA TTTGCTCATA TCGATTATGG GCCGCACTGT CGGCGCCCTC 
GGCAATCTGA CCTTCGTCCT GTGCATTATT ATCTTCATAT TCGCGGTCAT GGGCATGCAG 
CTGTTTGGCA AGAACTACAC AGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGAGCAT 
GGTGACAACG AACTGGAGCT GGGCCACGAC GAGATCCTCG CCGACGGCCT CATCAAGAAG 
GGGATCAAGG AGCAGACGCA ACTGGAGGTG GCCATCGGGG ATGGCATGGA ATTCACGATA 
CACGGCGACA TGAAGAACAA CAAGCCGAAG AAATCCAAAT ATCTAAATAA CGCAACGGAC 
GACGACACTG CCAGCATTAA CTCATATGGT AGCCATAAGA ATCGACCATT CAAGGACGAG 
AGCCACAAGG GCAGCGCCGA GACGATGGAG GGCGAGGAGA AGCGCGACGC CAGCAAGGAG 
GATTTAGGTC TCGACGAGGA ACTGGACGAG GAGGGCGAAT GCGAGGAGGG CCCGCTCGAC 
GGTGATATCA TTATTCATGC ACACGACGAG GATATACTCG ATGAATATCC AGCTGATTGC 
TGCCCCGATT CGTACTATAA GAAATTTCCG ATCTTAGCCG GTGACGATGA CTCGCCGTTC 
TGGCAAGGAT GGGGCAATTT ACGACTGAAA ACTTTTCAAT TAATTGAAAA TAAATATTTT 
GAAACAGCTG TTATCACTAT GATTTTAATG AGTAGCTTAG CTTTGGCATT AGAAGATGTA 
CATCTGCCAC AAAGACCCAT ACTGCAGGAT ATTTTATACT ATATGGACAG AATATTTACG 
GTTATATTCT TCTTGGAAAT GTTAATCAAG TGGTTGGCGC TCGGCTTCAA AGTGTACTTC 
ACCAACGCGT GGTGTTGGCT CGATTTCGTG ATTGTCATGG TATCGCTTAT CAACTTCGTT 
GCTTCACTTG TTGGAGCTGG TGGTATTCAA GCCTTCAAGA CTATGCGAAC GTTAAGAGCA 
CTGAGACCAC TACGTGCCAT GTCCCGTATG CAGGGCATGA GGGTCGTCGT TAATGCGCTG 
GTACAAGCTA TACCGTCCAT CTTCAATGTG CTATTGGTGT GTCTAATATT TTGGCTAATT 
TTTGCCATAA TGGGTGTACA GCTTTTTGCT GGAAAATATT TTAAGTGCGA GGACATGAAT 
GGCACGAAGC TCAGCCACGA GATCATACCA AATCGCAATG CCTGCGAGAG CGAGAACTAC 
ACGTGGGTGA ATTCAGCAAT GAATTTCGAT CATGTAGGTA ACGCGTATCT GTGCCTTTTC 
CAAGTGGCCA CCTTCAAAGG CTGGATACAA ATCATGAACG ATGCTATCGA TTCACGAGAG 
GTGGACAAGC AACCAATTCG TGAAACGAAC ATCTACATGT ATTTATATTT CGTATTCTTC 
ATCATATTTG GATCATTTTT CACACTCAAT CTGTTCATTG GTGTTATCAT TGATAATTTT 
AATGAGCAAA AGAAAAAAGC AGGTGGATCA TTAGAAATGT TCATGACAGA AGATCAGAAA 
AAGTACTATA ATGCTATGAA AAAGATGGGC TCTAAAAAAC CATTAAAAGC CATTCCAAGA 
CCAAGGTGGC GACCACAAGC AATAGTCTTT GAAATAGTAA CCGATAAGAA ATTCGATATA 
ATCATTATGT TATTCATTGG TCTGAACATG TTCACCATGA CCCTCGATCG TTACGATGCG 
TCGGACACGT ATAACGCGGT CCTAGACTAT CTCAATGCGA TATTCGTAGT TATTTTCAGT 
TCCGAATGTC TATTAAAAAT ATTCGCTTTA CGATATCACT ATTTTATTGA GCCATGGAAT 
TTATTTGATG TAGTAGTTGT CATTTTATCC ATCTTAGGTC TTGTACTTAG CGATATTATC 
GAGAAGTACT TCGTGTCGCC GACCCTGCTC CGAGTGGTGC GTGTGGCGAA AGTGGGCCGT 
GTCCTTCGAC TGGTGAAGGG AGCCAAGGGC ATTCGGACAC TGCTCTTCGC GTTGGCCATG 
TCGCTGCCGG CCCTGTTCAA CATCTGCCTG CTGCTGTTCC TGGTCATGTT CATCTTTGCC 
ATTTTCGGCA TGTCGTTCTT CATGCACGTG AAGGAGAAGA GCGGCATCAA CGACGTCTAC 
AACTTCAAGA CCTTTGGCCA GAGCATGATC CTGCTCTTTC AGATGTCGAC GTCAGCCGGT 
TGGGATGGTG TACTGGACGC CATTATCAAT GAGGAAGCAT GCGATCCACC CGACAACGAC 
AAAGGCTATC CGGGCAATTG TGGTTCAGCG ACCGTTGGAA TAACGTTTCT CCTCTCATAC 
CTAGTTATAA GCTTTTTGAT AGTTATTAAT ATGTACATTG CTGTCATTCT CGAGAACTAT 
AGTCAGGCCA CCGAGGACGT GCAAGAGGGT CTAACCGACG ACGACTACGA CATGTACTAT 
GAGATCTGGC AGCAATTCGA TCCGGAGGGC ACCCAGTACA TACGCTATGA TCAGCTGTCC 
GAATTCCTGG ACGTACTGGA GCCCCCGCTG CAGATCCACA AACCGAACAA GTACAAGATC 
ATATCGATGG ACATACCCAT CTGTCGCGGT GACCTCATGT ACTGCGTCGA CATCCTCGAC 
GCCCTTACGA AAGACTTCTT TGCGCGGAAG GGCAATCCGA TAGAGGAGAC GGGTGAGATT 
GGTGAGATAG CGGCCCGCCC GGATACGGAG GGCTACGAGC CCGTCTCATC AACGCTGTGG 
CGTCAGCGTG AGGAGTACTG CGCCCGGCTA ATCCAGCACG CCTGGCGAAA GCACAAGGCG 
CGCGGCGAGG GAGGTGGGTC CTTTGAGCCG GATACGGATC ATGGCGATGG CGGTGATCCG 
GATGCCGGGG ACCCGGCGCC CGATGAAGCA ACGGACGGCG ATGCGCCCGC TGGTGGAGAT 
GGTAGTGTTA ACGGTACTGC AGAAGGAGCT GCCGATGCCG ATGAGAGTAA TGTAAATAGT 
CCGGGTGAGG ATGCAGCGGC GGCGGCAGCA GCAGCAGCAG CAGCGGCGGC GGCGGGCACG 
ACGACGGCGG GAAGTCCCGG AGCGGGTAGC GCCGGGCGAC AGACCGCCGT TCTCGTGGAG 
AGCGACGGGT TCGTGACGAA GAACGGCCAC AAGGTGGTCA TCCACTCGCG ATCGCCGAGC 
ATCACGTCGC GCACGGCGGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001298403.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285332.1 Drosophila melanogaster paralytic (para), transcript variant BG, mRNA.

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CloneID	OFa03028
Clone ID Related Accession (Same CDS sequence)	NM_001298403.1 , NM_078647.4
Accession Version	NM_001298403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform BG
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGGCAAAG GCGTTTGTCG TTGTATATCT 
GCAGAGCATG GTGACAACGA ACTGGAGCTG GGCCACGACG AGATCCTCGC CGACGGCCTC 
ATCAAGAAGG GGATCAAGGA GCAGACGCAA CTGGAGGTGG CCATCGGGGA TGGCATGGAA 
TTCACGATAC ACGGCGACAT GAAGAACAAC AAGCCGAAGA AATCCAAATA TCTAAATAAC 
GCAACGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGGT ATCGCTTATC 
AACTTCGTTG CTTCACTTGT TGGAGCTGGT GGTATTCAAG CCTTCAAGAC TATGCGAACG 
TTAAGAGCAC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGCATGAG GGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285332.1
CDS	422..6817
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001298403.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant BG, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298403.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGGCAAAG GCGTTTGTCG TTGTATATCT 
GCAGAGCATG GTGACAACGA ACTGGAGCTG GGCCACGACG AGATCCTCGC CGACGGCCTC 
ATCAAGAAGG GGATCAAGGA GCAGACGCAA CTGGAGGTGG CCATCGGGGA TGGCATGGAA 
TTCACGATAC ACGGCGACAT GAAGAACAAC AAGCCGAAGA AATCCAAATA TCTAAATAAC 
GCAACGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGGT ATCGCTTATC 
AACTTCGTTG CTTCACTTGT TGGAGCTGGT GGTATTCAAG CCTTCAAGAC TATGCGAACG 
TTAAGAGCAC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGCATGAG GGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_078647.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523371.2 Drosophila melanogaster paralytic (para), transcript variant A, mRNA.

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CloneID	OFa03028
Clone ID Related Accession (Same CDS sequence)	NM_001298403.1 , NM_078647.4
Accession Version	NM_078647.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_078647.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGGCAAAG GCGTTTGTCG TTGTATATCT 
GCAGAGCATG GTGACAACGA ACTGGAGCTG GGCCACGACG AGATCCTCGC CGACGGCCTC 
ATCAAGAAGG GGATCAAGGA GCAGACGCAA CTGGAGGTGG CCATCGGGGA TGGCATGGAA 
TTCACGATAC ACGGCGACAT GAAGAACAAC AAGCCGAAGA AATCCAAATA TCTAAATAAC 
GCAACGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGGT ATCGCTTATC 
AACTTCGTTG CTTCACTTGT TGGAGCTGGT GGTATTCAAG CCTTCAAGAC TATGCGAACG 
TTAAGAGCAC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGCATGAG GGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523371.2
CDS	269..6664
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_078647.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078647.4

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGGCAAAG GCGTTTGTCG TTGTATATCT 
GCAGAGCATG GTGACAACGA ACTGGAGCTG GGCCACGACG AGATCCTCGC CGACGGCCTC 
ATCAAGAAGG GGATCAAGGA GCAGACGCAA CTGGAGGTGG CCATCGGGGA TGGCATGGAA 
TTCACGATAC ACGGCGACAT GAAGAACAAC AAGCCGAAGA AATCCAAATA TCTAAATAAC 
GCAACGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGGT ATCGCTTATC 
AACTTCGTTG CTTCACTTGT TGGAGCTGGT GGTATTCAAG CCTTCAAGAC TATGCGAACG 
TTAAGAGCAC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGCATGAG GGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001042815.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036280.1 Drosophila melanogaster paralytic (para), transcript variant B, mRNA.

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CloneID	OFa03029
Clone ID Related Accession (Same CDS sequence)	NM_001042815.2
Accession Version	NM_001042815.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_001042815.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGGCAAAG GCGTTTGTCG TTGTATATCT 
GCAGAGCATG GTGACAACGA ACTGGAGCTG GGCCACGACG AGATCCTCGC CGACGGCCTC 
ATCAAGAAGG GGATCAAGGA GCAGACGCAA CTGGAGGTGG CCATCGGGGA TGGCATGGAA 
TTCACGATAC ACGGCGACAT GAAGAACAAC AAGCCGAAGA AATCCAAATA TCTAAATAAC 
GCAACGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGCT GTCGCTAATT 
AATTTGGCCG CGGTCTGGTC CGGGGCCGAT GATGTGCCCG CCTTTCGCTC GATGCGAACT 
CTGCGCGCCC TGCGACCACT GCGGGCAGTT TCGCGCTGGG AGGGCATGAA AGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036280.1
CDS	269..6664
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001042815.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042815.2

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTATT CAAACTGGCC 
AAGTCTTGGC CCACACTTAA TTTACTCATT TCGATTATGG GACGCACCAT GGGCGCTTTG 
GGTAATCTGA CATTTGTACT TTGCATTATC ATCTTCATCT TTGCGGTGAT GGGAATGCAA 
CTGTTCGGAA AGAATTATCA TGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGGCAAAG GCGTTTGTCG TTGTATATCT 
GCAGAGCATG GTGACAACGA ACTGGAGCTG GGCCACGACG AGATCCTCGC CGACGGCCTC 
ATCAAGAAGG GGATCAAGGA GCAGACGCAA CTGGAGGTGG CCATCGGGGA TGGCATGGAA 
TTCACGATAC ACGGCGACAT GAAGAACAAC AAGCCGAAGA AATCCAAATA TCTAAATAAC 
GCAACGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGCT GTCGCTAATT 
AATTTGGCCG CGGTCTGGTC CGGGGCCGAT GATGTGCCCG CCTTTCGCTC GATGCGAACT 
CTGCGCGCCC TGCGACCACT GCGGGCAGTT TCGCGCTGGG AGGGCATGAA AGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001042816.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036281.1 Drosophila melanogaster paralytic (para), transcript variant C, mRNA.

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CloneID	OFa03030
Clone ID Related Accession (Same CDS sequence)	NM_001042816.2
Accession Version	NM_001042816.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_001042816.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTTTT CAAGCTGGCC 
AAATCGTGGC CGACGCTGAA TTTGCTCATA TCGATTATGG GCCGCACTGT CGGCGCCCTC 
GGCAATCTGA CCTTCGTCCT GTGCATTATT ATCTTCATAT TCGCGGTCAT GGGCATGCAG 
CTGTTTGGCA AGAACTACAC AGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGGCAAAG GCGTTTGTCG TTGTATATCT 
GCAGAGCATG GTGACAACGA ACTGGAGCTG GGCCACGACG AGATCCTCGC CGACGGCCTC 
ATCAAGAAGG GGATCAAGGA GCAGACGCAA CTGGAGGTGG CCATCGGGGA TGGCATGGAA 
TTCACGATAC ACGGCGACAT GAAGAACAAC AAGCCGAAGA AATCCAAATA TCTAAATAAC 
GCAACGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGGT ATCGCTTATC 
AACTTCGTTG CTTCACTTGT TGGAGCTGGT GGTATTCAAG CCTTCAAGAC TATGCGAACG 
TTAAGAGCAC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGCATGAG GGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036281.1
CDS	269..6664
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001042816.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042816.2

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGACATCCTT ATCCTTACCT 
GGTTCACCGT TTAACATACG CAGGGGATCA CGTAGTTCTC ACAAGTACAC GATACGGAAC 
GGACGTGGCC GCTTTGGTAT ACCCGGTAGC GATCGTAAGC CATTGGTATT GTCAACATAT 
CAGGATGCCC AGCAGCACTT GCCCTATGCC GACGACTCGA ATGCCGTCAC CCCGATGTCC 
GAAGAGAATG GGGCCATCAT AGTGCCCGTG TACTATGGCA ATCTAGGCTC CCGACACTCA 
TCGTATACCT CGCATCAGTC CCGAATATCG TATACCTCAC ATGGCGATCT ACTCGGCGGC 
ATGGCCGTCA TGGGCGTCAG CACAATGACC AAGGAGAGCA AATTGCGCAA CCGGAACACA 
CGCAATCAAT CAGTGGGCGC CACCAATGGC GGCACCACCT GTCTGGACAC CAATCACAAG 
CTCGATCATC GCGACTACGA AATTGGCCTG GAGTGCACGG ACGAAGCTGG CAAGATTAAA 
CATCATGACA ATCCTTTTAT CGAGCCCGTC CAGACACAAA CGGTGGTTGA TATGAAAGAT 
GTGATGGTCC TGAATGACAT CATCGAACAG GCCGCTGGTC GGCACAGTCG GGCAAGCGAT 
CGCGGTGTCT CCGTTTACTA TTTCCCAACA GAGGACGATG ACGAGGATGG GCCGACGTTC 
AAAGACAAGG CACTCGAAGT GATCCTCAAA GGCATCGATG TGTTTTGTGT GTGGGACTGT 
TGCTGGGTTT GGTTGAAATT TCAGGAGTGG GTATCGCTCA TCGTCTTCGA TCCCTTCGTC 
GAGCTCTTCA TCACGCTGTG CATTGTGGTC AACACGATGT TCATGGCAAT GGATCACCAC 
GATATGAACA AGGAGATGGA ACGCGTGCTC AAGAGTGGCA ACTATTTCTT CACCGCCACC 
TTTGCCATCG AGGCCACCAT GAAGCTAATG GCCATGAGCC CCAAGTACTA TTTCCAGGAG 
GGCTGGAACA TCTTCGACTT CATTATCGTG GCCCTATCGC TATTGGAACT GGGACTCGAG 
GGTGTCCAGG GTCTGTCCGT ATTGCGTTCC TTTCGATTGC TGCGTGTTTT CAAGCTGGCC 
AAATCGTGGC CGACGCTGAA TTTGCTCATA TCGATTATGG GCCGCACTGT CGGCGCCCTC 
GGCAATCTGA CCTTCGTCCT GTGCATTATT ATCTTCATAT TCGCGGTCAT GGGCATGCAG 
CTGTTTGGCA AGAACTACAC AGATCACAAG GACCGCTTTC CGGATGGCGA CCTGCCGCGC 
TGGAACTTCA CCGACTTTAT GCACAGCTTC ATGATCGTGT TCCGGGTGCT CTGCGGAGAA 
TGGATCGAGT CCATGTGGGA CTGCATGTAC GTGGGCGATG TCTCGTGCAT TCCCTTCTTC 
TTGGCCACCG TTGTCATCGG CAATCTTGTG GTACTTAACC TTTTCTTAGC CTTGCTTTTG 
TCCAATTTTG GCTCATCTAG CTTATCAGCG CCGACTGCCG ATAACGATAC GAATAAAATA 
GCCGAGGCCT TCAATCGAAT TGGCCGATTT AAAAGTTGGG TTAAGCGTAA TATTGCTGAT 
TGTTTCAAGT TAATACGTAA CAAATTGACA AATCAAATAA GTGATCAACC ATCAGGTGAG 
AGGACCAACC AGATCAGTTG GATTTGGAGC GAAGGCAAAG GCGTTTGTCG TTGTATATCT 
GCAGAGCATG GTGACAACGA ACTGGAGCTG GGCCACGACG AGATCCTCGC CGACGGCCTC 
ATCAAGAAGG GGATCAAGGA GCAGACGCAA CTGGAGGTGG CCATCGGGGA TGGCATGGAA 
TTCACGATAC ACGGCGACAT GAAGAACAAC AAGCCGAAGA AATCCAAATA TCTAAATAAC 
GCAACGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGGT ATCGCTTATC 
AACTTCGTTG CTTCACTTGT TGGAGCTGGT GGTATTCAAG CCTTCAAGAC TATGCGAACG 
TTAAGAGCAC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGCATGAG GGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201681.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188610.1 Drosophila melanogaster paralytic (para), transcript variant O, mRNA.

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CloneID	OFa03634
Clone ID Related Accession (Same CDS sequence)	NM_001201681.1
Accession Version	NM_001201681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform O
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGAGGACG ATGACGAGGA TGGGCCGACG TTCAAAGACA AGGCACTCGA AGTGATCCTC 
AAAGGCATCG ATGTGTTTTG TGTGTGGGAC TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG 
TGGGTATCGC TCATCGTCTT CGATCCCTTC GTCGAGCTCT TCATCACGCT GTGCATTGTG 
GTCAACACGA TGTTCATGGC AATGGATCAC CACGATATGA ACAAGGAGAT GGAACGCGTG 
CTCAAGAGTG GCAACTATTT CTTCACCGCC ACCTTTGCCA TCGAGGCCAC CATGAAGCTA 
ATGGCCATGA GCCCCAAGTA CTATTTCCAG GAGGGCTGGA ACATCTTCGA CTTCATTATC 
GTGGCCCTAT CGCTATTGGA ACTGGGACTC GAGGGTGTCC AGGGTCTGTC CGTATTGCGT 
TCCTTTCGAT TGCTGCGTGT ATTCAAACTG GCCAAGTCTT GGCCCACACT TAATTTACTC 
ATTTCGATTA TGGGACGCAC CATGGGCGCT TTGGGTAATC TGACATTTGT ACTTTGCATT 
ATCATCTTCA TCTTTGCGGT GATGGGAATG CAACTGTTCG GAAAGAATTA TCATGATCAC 
AAGGACCGCT TTCCGGATGG CGACCTGCCG CGCTGGAACT TCACCGACTT TATGCACAGC 
TTCATGATCG TGTTCCGGGT GCTCTGCGGA GAATGGATCG AGTCCATGTG GGACTGCATG 
TACGTGGGCG ATGTCTCGTG CATTCCCTTC TTCTTGGCCA CCGTTGTCAT CGGCAATCTT 
GTGGTACTTA ACCTTTTCTT AGCCTTGCTT TTGTCCAATT TTGGCTCATC TAGCTTATCA 
GCGCCGACTG CCGATAACGA TACGAATAAA ATAGCCGAGG CCTTCAATCG AATTGGCCGA 
TTTAAAAGTT GGGTTAAGCG TAATATTGCT GATTGTTTCA AGTTAATACG TAACAAATTG 
ACAAATCAAA TAAGTGATCA ACCATCAGGT GAGAGGACCA ACCAGATCAG TTGGATTTGG 
AGCGAAGAGC ATGGTGACAA CGAACTGGAG CTGGGCCACG ACGAGATCCT CGCCGACGGC 
CTCATCAAGA AGGGGATCAA GGAGCAGACG CAACTGGAGG TGGCCATCGG GGATGGCATG 
GAATTCACGA TACACGGCGA CATGAAGAAC AACAAGCCGA AGAAATCCAA ATATCTAAAT 
AACGCAACGG ACGACGACAC TGCCAGCATT AACTCATATG GTAGCCATAA GAATCGACCA 
TTCAAGGACG AGAGCCACAA GGGCAGCGCC GAGACGATGG AGGGCGAGGA GAAGCGCGAC 
GCCAGCAAGG AGGATTTAGG TCTCGACGAG GAACTGGACG AGGAGGGCGA ATGCGAGGAG 
GGCCCGCTCG ACGGTGATAT CATTATTCAT GCACACGACG AGGATATACT CGATGAATAT 
CCAGCTGATT GCTGCCCCGA TTCGTACTAT AAGAAATTTC CGATCTTAGC CGGTGACGAT 
GACTCGCCGT TCTGGCAAGG ATGGGGCAAT TTACGACTGA AAACTTTTCA ATTAATTGAA 
AATAAATATT TTGAAACAGC TGTTATCACT ATGATTTTAA TGAGTAGCTT AGCTTTGGCA 
TTAGAAGATG TACATCTGCC ACAAAGACCC ATACTGCAGG ATATTTTATA CTATATGGAC 
AGAATATTTA CGGTTATATT CTTCTTGGAA ATGTTAATCA AGTGGTTGGC GCTCGGCTTC 
AAAGTGTACT TCACCAACGC GTGGTGTTGG CTCGATTTCG TGATTGTCAT GGTATCGCTT 
ATCAACTTCG TTGCTTCACT TGTTGGAGCT GGTGGTATTC AAGCCTTCAA GACTATGCGA 
ACGTTAAGAG CACTGAGACC ACTACGTGCC ATGTCCCGTA TGCAGGGCAT GAGGGTCGTC 
GTTAATGCGC TGGTACAAGC TATACCGTCC ATCTTCAATG TGCTATTGGT GTGTCTAATA 
TTTTGGCTAA TTTTTGCCAT AATGGGTGTA CAGCTTTTTG CTGGAAAATA TTTTAAGTGC 
GAGGACATGA ATGGCACGAA GCTCAGCCAC GAGATCATAC CAAATCGCAA TGCCTGCGAG 
AGCGAGAACT ACACGTGGGT GAATTCAGCA ATGAATTTCG ATCATGTAGG TAACGCGTAT 
CTGTGCCTTT TCCAAGTGGC CACCTTCAAA GGCTGGATAC AAATCATGAA CGATGCTATC 
GATTCACGAG AGGTGGACAA GCAACCAATT CGTGAAACGA ACATCTACAT GTATTTATAT 
TTCGTATTCT TCATCATATT TGGATCATTT TTCACACTCA ATCTGTTCAT TGGTGTTATC 
ATTGATAATT TTAATGAGCA AAAGAAAAAA GCAGGTGGAT CATTAGAAAT GTTCATGACA 
GAAGATCAGA AAAAGTACTA TAATGCTATG AAAAAGATGG GCTCTAAAAA ACCATTAAAA 
GCCATTCCAA GACCAAGGTG GCGACCACAA GCAATAGTCT TTGAAATAGT AACCGATAAG 
AAATTCGATA TAATCATTAT GTTATTCATT GGTCTGAACA TGTTCACCAT GACCCTCGAT 
CGTTACGATG CGTCGGACAC GTATAACGCG GTCCTAGACT ATCTCAATGC GATATTCGTA 
GTTATTTTCA GTTCCGAATG TCTATTAAAA ATATTCGCTT TACGATATCA CTATTTTATT 
GAGCCATGGA ATTTATTTGA TGTAGTAGTT GTCATTTTAT CCATCTTAGG TCTTGTACTT 
AGCGATATTA TCGAGAAGTA CTTCGTGTCG CCGACCCTGC TCCGAGTGGT GCGTGTGGCG 
AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG GGAGCCAAGG GCATTCGGAC ACTGCTCTTC 
GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC AACATCTGCC TGCTGCTGTT CCTGGTCATG 
TTCATCTTTG CCATTTTCGG CATGTCGTTC TTCATGCACG TGAAGGAGAA GAGCGGCATC 
AACGACGTCT ACAACTTCAA GACCTTTGGC CAGAGCATGA TCCTGCTCTT TCAGATGTCG 
ACGTCAGCCG GTTGGGATGG TGTACTGGAC GCCATTATCA ATGAGGAAGC ATGCGATCCA 
CCCGACAACG ACAAAGGCTA TCCGGGCAAT TGTGGTTCAG CGACCGTTGG AATAACGTTT 
CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG ATAGTTATTA ATATGTACAT TGCTGTCATT 
CTCGAGAACT ATAGTCAGGC CACCGAGGAC GTGCAAGAGG GTCTAACCGA CGACGACTAC 
GACATGTACT ATGAGATCTG GCAGCAATTC GATCCGGAGG GCACCCAGTA CATACGCTAT 
GATCAGCTGT CCGAATTCCT GGACGTACTG GAGCCCCCGC TGCAGATCCA CAAACCGAAC 
AAGTACAAGA TCATATCGAT GGACATACCC ATCTGTCGCG GTGACCTCAT GTACTGCGTC 
GACATCCTCG ACGCCCTTAC GAAAGACTTC TTTGCGCGGA AGGGCAATCC GATAGAGGAG 
ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC CCGGATACGG AGGGCTACGA GCCCGTCTCA 
TCAACGCTGT GGCGTCAGCG TGAGGAGTAC TGCGCCCGGC TAATCCAGCA CGCCTGGCGA 
AAGCACAAGG CGCGCGGCGA GGGAGGTGGG TCCTTTGAGC CGGATACGGA TCATGGCGAT 
GGCGGTGATC CGGATGCCGG GGACCCGGCG CCCGATGAAG CAACGGACGG CGATGCGCCC 
GCTGGTGGAG ATGGTAGTGT TAACGGTACT GCAGAAGGAG CTGCCGATGC CGATGAGAGT 
AATGTAAATA GTCCGGGTGA GGATGCAGCG GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG 
GCGGCGGGCA CGACGACGGC GGGAAGTCCC GGAGCGGGTA GCGCCGGGCG ACAGACCGCC 
GTTCTCGTGG AGAGCGACGG GTTCGTGACG AAGAACGGCC ACAAGGTGGT CATCCACTCG 
CGATCGCCGA GCATCACGTC GCGCACGGCG GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188610.1
CDS	269..6547
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201681.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant O, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201681.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGAGGACG ATGACGAGGA TGGGCCGACG TTCAAAGACA AGGCACTCGA AGTGATCCTC 
AAAGGCATCG ATGTGTTTTG TGTGTGGGAC TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG 
TGGGTATCGC TCATCGTCTT CGATCCCTTC GTCGAGCTCT TCATCACGCT GTGCATTGTG 
GTCAACACGA TGTTCATGGC AATGGATCAC CACGATATGA ACAAGGAGAT GGAACGCGTG 
CTCAAGAGTG GCAACTATTT CTTCACCGCC ACCTTTGCCA TCGAGGCCAC CATGAAGCTA 
ATGGCCATGA GCCCCAAGTA CTATTTCCAG GAGGGCTGGA ACATCTTCGA CTTCATTATC 
GTGGCCCTAT CGCTATTGGA ACTGGGACTC GAGGGTGTCC AGGGTCTGTC CGTATTGCGT 
TCCTTTCGAT TGCTGCGTGT ATTCAAACTG GCCAAGTCTT GGCCCACACT TAATTTACTC 
ATTTCGATTA TGGGACGCAC CATGGGCGCT TTGGGTAATC TGACATTTGT ACTTTGCATT 
ATCATCTTCA TCTTTGCGGT GATGGGAATG CAACTGTTCG GAAAGAATTA TCATGATCAC 
AAGGACCGCT TTCCGGATGG CGACCTGCCG CGCTGGAACT TCACCGACTT TATGCACAGC 
TTCATGATCG TGTTCCGGGT GCTCTGCGGA GAATGGATCG AGTCCATGTG GGACTGCATG 
TACGTGGGCG ATGTCTCGTG CATTCCCTTC TTCTTGGCCA CCGTTGTCAT CGGCAATCTT 
GTGGTACTTA ACCTTTTCTT AGCCTTGCTT TTGTCCAATT TTGGCTCATC TAGCTTATCA 
GCGCCGACTG CCGATAACGA TACGAATAAA ATAGCCGAGG CCTTCAATCG AATTGGCCGA 
TTTAAAAGTT GGGTTAAGCG TAATATTGCT GATTGTTTCA AGTTAATACG TAACAAATTG 
ACAAATCAAA TAAGTGATCA ACCATCAGGT GAGAGGACCA ACCAGATCAG TTGGATTTGG 
AGCGAAGAGC ATGGTGACAA CGAACTGGAG CTGGGCCACG ACGAGATCCT CGCCGACGGC 
CTCATCAAGA AGGGGATCAA GGAGCAGACG CAACTGGAGG TGGCCATCGG GGATGGCATG 
GAATTCACGA TACACGGCGA CATGAAGAAC AACAAGCCGA AGAAATCCAA ATATCTAAAT 
AACGCAACGG ACGACGACAC TGCCAGCATT AACTCATATG GTAGCCATAA GAATCGACCA 
TTCAAGGACG AGAGCCACAA GGGCAGCGCC GAGACGATGG AGGGCGAGGA GAAGCGCGAC 
GCCAGCAAGG AGGATTTAGG TCTCGACGAG GAACTGGACG AGGAGGGCGA ATGCGAGGAG 
GGCCCGCTCG ACGGTGATAT CATTATTCAT GCACACGACG AGGATATACT CGATGAATAT 
CCAGCTGATT GCTGCCCCGA TTCGTACTAT AAGAAATTTC CGATCTTAGC CGGTGACGAT 
GACTCGCCGT TCTGGCAAGG ATGGGGCAAT TTACGACTGA AAACTTTTCA ATTAATTGAA 
AATAAATATT TTGAAACAGC TGTTATCACT ATGATTTTAA TGAGTAGCTT AGCTTTGGCA 
TTAGAAGATG TACATCTGCC ACAAAGACCC ATACTGCAGG ATATTTTATA CTATATGGAC 
AGAATATTTA CGGTTATATT CTTCTTGGAA ATGTTAATCA AGTGGTTGGC GCTCGGCTTC 
AAAGTGTACT TCACCAACGC GTGGTGTTGG CTCGATTTCG TGATTGTCAT GGTATCGCTT 
ATCAACTTCG TTGCTTCACT TGTTGGAGCT GGTGGTATTC AAGCCTTCAA GACTATGCGA 
ACGTTAAGAG CACTGAGACC ACTACGTGCC ATGTCCCGTA TGCAGGGCAT GAGGGTCGTC 
GTTAATGCGC TGGTACAAGC TATACCGTCC ATCTTCAATG TGCTATTGGT GTGTCTAATA 
TTTTGGCTAA TTTTTGCCAT AATGGGTGTA CAGCTTTTTG CTGGAAAATA TTTTAAGTGC 
GAGGACATGA ATGGCACGAA GCTCAGCCAC GAGATCATAC CAAATCGCAA TGCCTGCGAG 
AGCGAGAACT ACACGTGGGT GAATTCAGCA ATGAATTTCG ATCATGTAGG TAACGCGTAT 
CTGTGCCTTT TCCAAGTGGC CACCTTCAAA GGCTGGATAC AAATCATGAA CGATGCTATC 
GATTCACGAG AGGTGGACAA GCAACCAATT CGTGAAACGA ACATCTACAT GTATTTATAT 
TTCGTATTCT TCATCATATT TGGATCATTT TTCACACTCA ATCTGTTCAT TGGTGTTATC 
ATTGATAATT TTAATGAGCA AAAGAAAAAA GCAGGTGGAT CATTAGAAAT GTTCATGACA 
GAAGATCAGA AAAAGTACTA TAATGCTATG AAAAAGATGG GCTCTAAAAA ACCATTAAAA 
GCCATTCCAA GACCAAGGTG GCGACCACAA GCAATAGTCT TTGAAATAGT AACCGATAAG 
AAATTCGATA TAATCATTAT GTTATTCATT GGTCTGAACA TGTTCACCAT GACCCTCGAT 
CGTTACGATG CGTCGGACAC GTATAACGCG GTCCTAGACT ATCTCAATGC GATATTCGTA 
GTTATTTTCA GTTCCGAATG TCTATTAAAA ATATTCGCTT TACGATATCA CTATTTTATT 
GAGCCATGGA ATTTATTTGA TGTAGTAGTT GTCATTTTAT CCATCTTAGG TCTTGTACTT 
AGCGATATTA TCGAGAAGTA CTTCGTGTCG CCGACCCTGC TCCGAGTGGT GCGTGTGGCG 
AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG GGAGCCAAGG GCATTCGGAC ACTGCTCTTC 
GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC AACATCTGCC TGCTGCTGTT CCTGGTCATG 
TTCATCTTTG CCATTTTCGG CATGTCGTTC TTCATGCACG TGAAGGAGAA GAGCGGCATC 
AACGACGTCT ACAACTTCAA GACCTTTGGC CAGAGCATGA TCCTGCTCTT TCAGATGTCG 
ACGTCAGCCG GTTGGGATGG TGTACTGGAC GCCATTATCA ATGAGGAAGC ATGCGATCCA 
CCCGACAACG ACAAAGGCTA TCCGGGCAAT TGTGGTTCAG CGACCGTTGG AATAACGTTT 
CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG ATAGTTATTA ATATGTACAT TGCTGTCATT 
CTCGAGAACT ATAGTCAGGC CACCGAGGAC GTGCAAGAGG GTCTAACCGA CGACGACTAC 
GACATGTACT ATGAGATCTG GCAGCAATTC GATCCGGAGG GCACCCAGTA CATACGCTAT 
GATCAGCTGT CCGAATTCCT GGACGTACTG GAGCCCCCGC TGCAGATCCA CAAACCGAAC 
AAGTACAAGA TCATATCGAT GGACATACCC ATCTGTCGCG GTGACCTCAT GTACTGCGTC 
GACATCCTCG ACGCCCTTAC GAAAGACTTC TTTGCGCGGA AGGGCAATCC GATAGAGGAG 
ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC CCGGATACGG AGGGCTACGA GCCCGTCTCA 
TCAACGCTGT GGCGTCAGCG TGAGGAGTAC TGCGCCCGGC TAATCCAGCA CGCCTGGCGA 
AAGCACAAGG CGCGCGGCGA GGGAGGTGGG TCCTTTGAGC CGGATACGGA TCATGGCGAT 
GGCGGTGATC CGGATGCCGG GGACCCGGCG CCCGATGAAG CAACGGACGG CGATGCGCCC 
GCTGGTGGAG ATGGTAGTGT TAACGGTACT GCAGAAGGAG CTGCCGATGC CGATGAGAGT 
AATGTAAATA GTCCGGGTGA GGATGCAGCG GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG 
GCGGCGGGCA CGACGACGGC GGGAAGTCCC GGAGCGGGTA GCGCCGGGCG ACAGACCGCC 
GTTCTCGTGG AGAGCGACGG GTTCGTGACG AAGAACGGCC ACAAGGTGGT CATCCACTCG 
CGATCGCCGA GCATCACGTC GCGCACGGCG GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201703.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188632.1 Drosophila melanogaster paralytic (para), transcript variant AK, mRNA.

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CloneID	OFa03642
Clone ID Related Accession (Same CDS sequence)	NM_001201703.3 , NM_001201674.1
Accession Version	NM_001201703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AK
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001201703.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGAGCATGGT GACAACGAAC TGGAGCTGGG CCACGACGAG 
ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG ATCAAGGAGC AGACGCAACT GGAGGTGGCC 
ATCGGGGATG GCATGGAATT CACGATACAC GGCGACATGA AGAACAACAA GCCGAAGAAA 
TCCAAATATC TAAATAACGC AACGGACGAC GACACTGCCA GCATTAACTC ATATGGTAGC 
CATAAGAATC GACCATTCAA GGACGAGAGC CACAAGGGCA GCGCCGAGAC GATGGAGGGC 
GAGGAGAAGC GCGACGCCAG CAAGGAGGAT TTAGGTCTCG ACGAGGAACT GGACGAGGAG 
GGCGAATGCG AGGAGGGCCC GCTCGACGGT GATATCATTA TTCATGCACA CGACGAGGAT 
ATACTCGATG AATATCCAGC TGATTGCTGC CCCGATTCGT ACTATAAGAA ATTTCCGATC 
TTAGCCGGTG ACGATGACTC GCCGTTCTGG CAAGGATGGG GCAATTTACG ACTGAAAACT 
TTTCAATTAA TTGAAAATAA ATATTTTGAA ACAGCTGTTA TCACTATGAT TTTAATGAGT 
AGCTTAGCTT TGGCATTAGA AGATGTACAT CTGCCACAAA GACCCATACT GCAGGATATT 
TTATACTATA TGGACAGAAT ATTTACGGTT ATATTCTTCT TGGAAATGTT AATCAAGTGG 
TTGGCGCTCG GCTTCAAAGT GTACTTCACC AACGCGTGGT GTTGGCTCGA TTTCGTGATT 
GTCATGGTAT CGCTTATCAA CTTCGTTGCT TCACTTGTTG GAGCTGGTGG TATTCAAGCC 
TTCAAGACTA TGCGAACGTT AAGAGCACTG AGACCACTAC GTGCCATGTC CCGTATGCAG 
GGCATGAGGG TCGTCGTTAA TGCGCTGGTA CAAGCTATAC CGTCCATCTT CAATGTGCTA 
TTGGTGTGTC TAATATTTTG GCTAATTTTT GCCATAATGG GTGTACAGCT TTTTGCTGGA 
AAATATTTTA AGTGCGAGGA CATGAATGGC ACGAAGCTCA GCCACGAGAT CATACCAAAT 
CGCAATGCCT GCGAGAGCGA GAACTACACG TGGGTGAATT CAGCAATGAA TTTCGATCAT 
GTAGGTAACG CGTATCTGTG CCTTTTCCAA GTGGCCACCT TCAAAGGCTG GATACAAATC 
ATGAACGATG CTATCGATTC ACGAGAGGTG GACAAGCAAC CAATTCGTGA AACGAACATC 
TACATGTATT TATATTTCGT ATTCTTCATC ATATTTGGAT CATTTTTCAC ACTCAATCTG 
TTCATTGGTG TTATCATTGA TAATTTTAAT GAGCAAAAGA AAAAAGCAGG TGGATCATTA 
GAAATGTTCA TGACAGAAGA TCAGAAAAAG TACTATAATG CTATGAAAAA GATGGGCTCT 
AAAAAACCAT TAAAAGCCAT TCCAAGACCA AGGTGGCGAC CACAAGCAAT AGTCTTTGAA 
ATAGTAACCG ATAAGAAATT CGATATAATC ATTATGTTAT TCATTGGTCT GAACATGTTC 
ACCATGACCC TCGATCGTTA CGATGCGTCG GACACGTATA ACGCGGTCCT AGACTATCTC 
AATGCGATAT TCGTAGTTAT TTTCAGTTCC GAATGTCTAT TAAAAATATT CGCTTTACGA 
TATCACTATT TTATTGAGCC ATGGAATTTA TTTGATGTAG TAGTTGTCAT TTTATCCATC 
TTAGGTCTTG TACTTAGCGA TATTATCGAG AAGTACTTCG TGTCGCCGAC CCTGCTCCGA 
GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC CTTCGACTGG TGAAGGGAGC CAAGGGCATT 
CGGACACTGC TCTTCGCGTT GGCCATGTCG CTGCCGGCCC TGTTCAACAT CTGCCTGCTG 
CTGTTCCTGG TCATGTTCAT CTTTGCCATT TTCGGCATGT CGTTCTTCAT GCACGTGAAG 
GAGAAGAGCG GCATCAACGA CGTCTACAAC TTCAAGACCT TTGGCCAGAG CATGATCCTG 
CTCTTTCAGA TGTCGACGTC AGCCGGTTGG GATGGTGTAC TGGACGCCAT TATCAATGAG 
GAAGCATGCG ATCCACCCGA CAACGACAAA GGCTATCCGG GCAATTGTGG TTCAGCGACC 
GTTGGAATAA CGTTTCTCCT CTCATACCTA GTTATAAGCT TTTTGATAGT TATTAATATG 
TACATTGCTG TCATTCTCGA GAACTATAGT CAGGCCACCG AGGACGTGCA AGAGGGTCTA 
ACCGACGACG ACTACGACAT GTACTATGAG ATCTGGCAGC AATTCGATCC GGAGGGCACC 
CAGTACATAC GCTATGATCA GCTGTCCGAA TTCCTGGACG TACTGGAGCC CCCGCTGCAG 
ATCCACAAAC CGAACAAGTA CAAGATCATA TCGATGGACA TACCCATCTG TCGCGGTGAC 
CTCATGTACT GCGTCGACAT CCTCGACGCC CTTACGAAAG ACTTCTTTGC GCGGAAGGGC 
AATCCGATAG AGGAGACGGG TGAGATTGGT GAGATAGCGG CCCGCCCGGA TACGGAGGGC 
TACGAGCCCG TCTCATCAAC GCTGTGGCGT CAGCGTGAGG AGTACTGCGC CCGGCTAATC 
CAGCACGCCT GGCGAAAGCA CAAGGCGCGC GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT 
ACGGATCATG GCGATGGCGG TGATCCGGAT GCCGGGGACC CGGCGCCCGA TGAAGCAACG 
GACGGCGATG CGCCCGCTGG TGGAGATGGT AGTGTTAACG GTACTGCAGA AGGAGCTGCC 
GATGCCGATG AGAGTAATGT AAATAGTCCG GGTGAGGATG CAGCGGCGGC GGCAGCAGCA 
GCAGCAGCAG CGGCGGCGGC GGGCACGACG ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC 
GGGCGACAGA CCGCCGTTCT CGTGGAGAGC GACGGGTTCG TGACGAAGAA CGGCCACAAG 
GTGGTCATCC ACTCGCGATC GCCGAGCATC ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188632.1
CDS	792..7085
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201703.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AK, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201703.3

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGAGCATGGT GACAACGAAC TGGAGCTGGG CCACGACGAG 
ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG ATCAAGGAGC AGACGCAACT GGAGGTGGCC 
ATCGGGGATG GCATGGAATT CACGATACAC GGCGACATGA AGAACAACAA GCCGAAGAAA 
TCCAAATATC TAAATAACGC AACGGACGAC GACACTGCCA GCATTAACTC ATATGGTAGC 
CATAAGAATC GACCATTCAA GGACGAGAGC CACAAGGGCA GCGCCGAGAC GATGGAGGGC 
GAGGAGAAGC GCGACGCCAG CAAGGAGGAT TTAGGTCTCG ACGAGGAACT GGACGAGGAG 
GGCGAATGCG AGGAGGGCCC GCTCGACGGT GATATCATTA TTCATGCACA CGACGAGGAT 
ATACTCGATG AATATCCAGC TGATTGCTGC CCCGATTCGT ACTATAAGAA ATTTCCGATC 
TTAGCCGGTG ACGATGACTC GCCGTTCTGG CAAGGATGGG GCAATTTACG ACTGAAAACT 
TTTCAATTAA TTGAAAATAA ATATTTTGAA ACAGCTGTTA TCACTATGAT TTTAATGAGT 
AGCTTAGCTT TGGCATTAGA AGATGTACAT CTGCCACAAA GACCCATACT GCAGGATATT 
TTATACTATA TGGACAGAAT ATTTACGGTT ATATTCTTCT TGGAAATGTT AATCAAGTGG 
TTGGCGCTCG GCTTCAAAGT GTACTTCACC AACGCGTGGT GTTGGCTCGA TTTCGTGATT 
GTCATGGTAT CGCTTATCAA CTTCGTTGCT TCACTTGTTG GAGCTGGTGG TATTCAAGCC 
TTCAAGACTA TGCGAACGTT AAGAGCACTG AGACCACTAC GTGCCATGTC CCGTATGCAG 
GGCATGAGGG TCGTCGTTAA TGCGCTGGTA CAAGCTATAC CGTCCATCTT CAATGTGCTA 
TTGGTGTGTC TAATATTTTG GCTAATTTTT GCCATAATGG GTGTACAGCT TTTTGCTGGA 
AAATATTTTA AGTGCGAGGA CATGAATGGC ACGAAGCTCA GCCACGAGAT CATACCAAAT 
CGCAATGCCT GCGAGAGCGA GAACTACACG TGGGTGAATT CAGCAATGAA TTTCGATCAT 
GTAGGTAACG CGTATCTGTG CCTTTTCCAA GTGGCCACCT TCAAAGGCTG GATACAAATC 
ATGAACGATG CTATCGATTC ACGAGAGGTG GACAAGCAAC CAATTCGTGA AACGAACATC 
TACATGTATT TATATTTCGT ATTCTTCATC ATATTTGGAT CATTTTTCAC ACTCAATCTG 
TTCATTGGTG TTATCATTGA TAATTTTAAT GAGCAAAAGA AAAAAGCAGG TGGATCATTA 
GAAATGTTCA TGACAGAAGA TCAGAAAAAG TACTATAATG CTATGAAAAA GATGGGCTCT 
AAAAAACCAT TAAAAGCCAT TCCAAGACCA AGGTGGCGAC CACAAGCAAT AGTCTTTGAA 
ATAGTAACCG ATAAGAAATT CGATATAATC ATTATGTTAT TCATTGGTCT GAACATGTTC 
ACCATGACCC TCGATCGTTA CGATGCGTCG GACACGTATA ACGCGGTCCT AGACTATCTC 
AATGCGATAT TCGTAGTTAT TTTCAGTTCC GAATGTCTAT TAAAAATATT CGCTTTACGA 
TATCACTATT TTATTGAGCC ATGGAATTTA TTTGATGTAG TAGTTGTCAT TTTATCCATC 
TTAGGTCTTG TACTTAGCGA TATTATCGAG AAGTACTTCG TGTCGCCGAC CCTGCTCCGA 
GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC CTTCGACTGG TGAAGGGAGC CAAGGGCATT 
CGGACACTGC TCTTCGCGTT GGCCATGTCG CTGCCGGCCC TGTTCAACAT CTGCCTGCTG 
CTGTTCCTGG TCATGTTCAT CTTTGCCATT TTCGGCATGT CGTTCTTCAT GCACGTGAAG 
GAGAAGAGCG GCATCAACGA CGTCTACAAC TTCAAGACCT TTGGCCAGAG CATGATCCTG 
CTCTTTCAGA TGTCGACGTC AGCCGGTTGG GATGGTGTAC TGGACGCCAT TATCAATGAG 
GAAGCATGCG ATCCACCCGA CAACGACAAA GGCTATCCGG GCAATTGTGG TTCAGCGACC 
GTTGGAATAA CGTTTCTCCT CTCATACCTA GTTATAAGCT TTTTGATAGT TATTAATATG 
TACATTGCTG TCATTCTCGA GAACTATAGT CAGGCCACCG AGGACGTGCA AGAGGGTCTA 
ACCGACGACG ACTACGACAT GTACTATGAG ATCTGGCAGC AATTCGATCC GGAGGGCACC 
CAGTACATAC GCTATGATCA GCTGTCCGAA TTCCTGGACG TACTGGAGCC CCCGCTGCAG 
ATCCACAAAC CGAACAAGTA CAAGATCATA TCGATGGACA TACCCATCTG TCGCGGTGAC 
CTCATGTACT GCGTCGACAT CCTCGACGCC CTTACGAAAG ACTTCTTTGC GCGGAAGGGC 
AATCCGATAG AGGAGACGGG TGAGATTGGT GAGATAGCGG CCCGCCCGGA TACGGAGGGC 
TACGAGCCCG TCTCATCAAC GCTGTGGCGT CAGCGTGAGG AGTACTGCGC CCGGCTAATC 
CAGCACGCCT GGCGAAAGCA CAAGGCGCGC GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT 
ACGGATCATG GCGATGGCGG TGATCCGGAT GCCGGGGACC CGGCGCCCGA TGAAGCAACG 
GACGGCGATG CGCCCGCTGG TGGAGATGGT AGTGTTAACG GTACTGCAGA AGGAGCTGCC 
GATGCCGATG AGAGTAATGT AAATAGTCCG GGTGAGGATG CAGCGGCGGC GGCAGCAGCA 
GCAGCAGCAG CGGCGGCGGC GGGCACGACG ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC 
GGGCGACAGA CCGCCGTTCT CGTGGAGAGC GACGGGTTCG TGACGAAGAA CGGCCACAAG 
GTGGTCATCC ACTCGCGATC GCCGAGCATC ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201674.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188603.1 Drosophila melanogaster paralytic (para), transcript variant H, mRNA.

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CloneID	OFa03642
Clone ID Related Accession (Same CDS sequence)	NM_001201703.3 , NM_001201674.1
Accession Version	NM_001201674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGAGCATGGT GACAACGAAC TGGAGCTGGG CCACGACGAG 
ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG ATCAAGGAGC AGACGCAACT GGAGGTGGCC 
ATCGGGGATG GCATGGAATT CACGATACAC GGCGACATGA AGAACAACAA GCCGAAGAAA 
TCCAAATATC TAAATAACGC AACGGACGAC GACACTGCCA GCATTAACTC ATATGGTAGC 
CATAAGAATC GACCATTCAA GGACGAGAGC CACAAGGGCA GCGCCGAGAC GATGGAGGGC 
GAGGAGAAGC GCGACGCCAG CAAGGAGGAT TTAGGTCTCG ACGAGGAACT GGACGAGGAG 
GGCGAATGCG AGGAGGGCCC GCTCGACGGT GATATCATTA TTCATGCACA CGACGAGGAT 
ATACTCGATG AATATCCAGC TGATTGCTGC CCCGATTCGT ACTATAAGAA ATTTCCGATC 
TTAGCCGGTG ACGATGACTC GCCGTTCTGG CAAGGATGGG GCAATTTACG ACTGAAAACT 
TTTCAATTAA TTGAAAATAA ATATTTTGAA ACAGCTGTTA TCACTATGAT TTTAATGAGT 
AGCTTAGCTT TGGCATTAGA AGATGTACAT CTGCCACAAA GACCCATACT GCAGGATATT 
TTATACTATA TGGACAGAAT ATTTACGGTT ATATTCTTCT TGGAAATGTT AATCAAGTGG 
TTGGCGCTCG GCTTCAAAGT GTACTTCACC AACGCGTGGT GTTGGCTCGA TTTCGTGATT 
GTCATGGTAT CGCTTATCAA CTTCGTTGCT TCACTTGTTG GAGCTGGTGG TATTCAAGCC 
TTCAAGACTA TGCGAACGTT AAGAGCACTG AGACCACTAC GTGCCATGTC CCGTATGCAG 
GGCATGAGGG TCGTCGTTAA TGCGCTGGTA CAAGCTATAC CGTCCATCTT CAATGTGCTA 
TTGGTGTGTC TAATATTTTG GCTAATTTTT GCCATAATGG GTGTACAGCT TTTTGCTGGA 
AAATATTTTA AGTGCGAGGA CATGAATGGC ACGAAGCTCA GCCACGAGAT CATACCAAAT 
CGCAATGCCT GCGAGAGCGA GAACTACACG TGGGTGAATT CAGCAATGAA TTTCGATCAT 
GTAGGTAACG CGTATCTGTG CCTTTTCCAA GTGGCCACCT TCAAAGGCTG GATACAAATC 
ATGAACGATG CTATCGATTC ACGAGAGGTG GACAAGCAAC CAATTCGTGA AACGAACATC 
TACATGTATT TATATTTCGT ATTCTTCATC ATATTTGGAT CATTTTTCAC ACTCAATCTG 
TTCATTGGTG TTATCATTGA TAATTTTAAT GAGCAAAAGA AAAAAGCAGG TGGATCATTA 
GAAATGTTCA TGACAGAAGA TCAGAAAAAG TACTATAATG CTATGAAAAA GATGGGCTCT 
AAAAAACCAT TAAAAGCCAT TCCAAGACCA AGGTGGCGAC CACAAGCAAT AGTCTTTGAA 
ATAGTAACCG ATAAGAAATT CGATATAATC ATTATGTTAT TCATTGGTCT GAACATGTTC 
ACCATGACCC TCGATCGTTA CGATGCGTCG GACACGTATA ACGCGGTCCT AGACTATCTC 
AATGCGATAT TCGTAGTTAT TTTCAGTTCC GAATGTCTAT TAAAAATATT CGCTTTACGA 
TATCACTATT TTATTGAGCC ATGGAATTTA TTTGATGTAG TAGTTGTCAT TTTATCCATC 
TTAGGTCTTG TACTTAGCGA TATTATCGAG AAGTACTTCG TGTCGCCGAC CCTGCTCCGA 
GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC CTTCGACTGG TGAAGGGAGC CAAGGGCATT 
CGGACACTGC TCTTCGCGTT GGCCATGTCG CTGCCGGCCC TGTTCAACAT CTGCCTGCTG 
CTGTTCCTGG TCATGTTCAT CTTTGCCATT TTCGGCATGT CGTTCTTCAT GCACGTGAAG 
GAGAAGAGCG GCATCAACGA CGTCTACAAC TTCAAGACCT TTGGCCAGAG CATGATCCTG 
CTCTTTCAGA TGTCGACGTC AGCCGGTTGG GATGGTGTAC TGGACGCCAT TATCAATGAG 
GAAGCATGCG ATCCACCCGA CAACGACAAA GGCTATCCGG GCAATTGTGG TTCAGCGACC 
GTTGGAATAA CGTTTCTCCT CTCATACCTA GTTATAAGCT TTTTGATAGT TATTAATATG 
TACATTGCTG TCATTCTCGA GAACTATAGT CAGGCCACCG AGGACGTGCA AGAGGGTCTA 
ACCGACGACG ACTACGACAT GTACTATGAG ATCTGGCAGC AATTCGATCC GGAGGGCACC 
CAGTACATAC GCTATGATCA GCTGTCCGAA TTCCTGGACG TACTGGAGCC CCCGCTGCAG 
ATCCACAAAC CGAACAAGTA CAAGATCATA TCGATGGACA TACCCATCTG TCGCGGTGAC 
CTCATGTACT GCGTCGACAT CCTCGACGCC CTTACGAAAG ACTTCTTTGC GCGGAAGGGC 
AATCCGATAG AGGAGACGGG TGAGATTGGT GAGATAGCGG CCCGCCCGGA TACGGAGGGC 
TACGAGCCCG TCTCATCAAC GCTGTGGCGT CAGCGTGAGG AGTACTGCGC CCGGCTAATC 
CAGCACGCCT GGCGAAAGCA CAAGGCGCGC GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT 
ACGGATCATG GCGATGGCGG TGATCCGGAT GCCGGGGACC CGGCGCCCGA TGAAGCAACG 
GACGGCGATG CGCCCGCTGG TGGAGATGGT AGTGTTAACG GTACTGCAGA AGGAGCTGCC 
GATGCCGATG AGAGTAATGT AAATAGTCCG GGTGAGGATG CAGCGGCGGC GGCAGCAGCA 
GCAGCAGCAG CGGCGGCGGC GGGCACGACG ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC 
GGGCGACAGA CCGCCGTTCT CGTGGAGAGC GACGGGTTCG TGACGAAGAA CGGCCACAAG 
GTGGTCATCC ACTCGCGATC GCCGAGCATC ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188603.1
CDS	269..6562
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201674.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201674.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGAGCATGGT GACAACGAAC TGGAGCTGGG CCACGACGAG 
ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG ATCAAGGAGC AGACGCAACT GGAGGTGGCC 
ATCGGGGATG GCATGGAATT CACGATACAC GGCGACATGA AGAACAACAA GCCGAAGAAA 
TCCAAATATC TAAATAACGC AACGGACGAC GACACTGCCA GCATTAACTC ATATGGTAGC 
CATAAGAATC GACCATTCAA GGACGAGAGC CACAAGGGCA GCGCCGAGAC GATGGAGGGC 
GAGGAGAAGC GCGACGCCAG CAAGGAGGAT TTAGGTCTCG ACGAGGAACT GGACGAGGAG 
GGCGAATGCG AGGAGGGCCC GCTCGACGGT GATATCATTA TTCATGCACA CGACGAGGAT 
ATACTCGATG AATATCCAGC TGATTGCTGC CCCGATTCGT ACTATAAGAA ATTTCCGATC 
TTAGCCGGTG ACGATGACTC GCCGTTCTGG CAAGGATGGG GCAATTTACG ACTGAAAACT 
TTTCAATTAA TTGAAAATAA ATATTTTGAA ACAGCTGTTA TCACTATGAT TTTAATGAGT 
AGCTTAGCTT TGGCATTAGA AGATGTACAT CTGCCACAAA GACCCATACT GCAGGATATT 
TTATACTATA TGGACAGAAT ATTTACGGTT ATATTCTTCT TGGAAATGTT AATCAAGTGG 
TTGGCGCTCG GCTTCAAAGT GTACTTCACC AACGCGTGGT GTTGGCTCGA TTTCGTGATT 
GTCATGGTAT CGCTTATCAA CTTCGTTGCT TCACTTGTTG GAGCTGGTGG TATTCAAGCC 
TTCAAGACTA TGCGAACGTT AAGAGCACTG AGACCACTAC GTGCCATGTC CCGTATGCAG 
GGCATGAGGG TCGTCGTTAA TGCGCTGGTA CAAGCTATAC CGTCCATCTT CAATGTGCTA 
TTGGTGTGTC TAATATTTTG GCTAATTTTT GCCATAATGG GTGTACAGCT TTTTGCTGGA 
AAATATTTTA AGTGCGAGGA CATGAATGGC ACGAAGCTCA GCCACGAGAT CATACCAAAT 
CGCAATGCCT GCGAGAGCGA GAACTACACG TGGGTGAATT CAGCAATGAA TTTCGATCAT 
GTAGGTAACG CGTATCTGTG CCTTTTCCAA GTGGCCACCT TCAAAGGCTG GATACAAATC 
ATGAACGATG CTATCGATTC ACGAGAGGTG GACAAGCAAC CAATTCGTGA AACGAACATC 
TACATGTATT TATATTTCGT ATTCTTCATC ATATTTGGAT CATTTTTCAC ACTCAATCTG 
TTCATTGGTG TTATCATTGA TAATTTTAAT GAGCAAAAGA AAAAAGCAGG TGGATCATTA 
GAAATGTTCA TGACAGAAGA TCAGAAAAAG TACTATAATG CTATGAAAAA GATGGGCTCT 
AAAAAACCAT TAAAAGCCAT TCCAAGACCA AGGTGGCGAC CACAAGCAAT AGTCTTTGAA 
ATAGTAACCG ATAAGAAATT CGATATAATC ATTATGTTAT TCATTGGTCT GAACATGTTC 
ACCATGACCC TCGATCGTTA CGATGCGTCG GACACGTATA ACGCGGTCCT AGACTATCTC 
AATGCGATAT TCGTAGTTAT TTTCAGTTCC GAATGTCTAT TAAAAATATT CGCTTTACGA 
TATCACTATT TTATTGAGCC ATGGAATTTA TTTGATGTAG TAGTTGTCAT TTTATCCATC 
TTAGGTCTTG TACTTAGCGA TATTATCGAG AAGTACTTCG TGTCGCCGAC CCTGCTCCGA 
GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC CTTCGACTGG TGAAGGGAGC CAAGGGCATT 
CGGACACTGC TCTTCGCGTT GGCCATGTCG CTGCCGGCCC TGTTCAACAT CTGCCTGCTG 
CTGTTCCTGG TCATGTTCAT CTTTGCCATT TTCGGCATGT CGTTCTTCAT GCACGTGAAG 
GAGAAGAGCG GCATCAACGA CGTCTACAAC TTCAAGACCT TTGGCCAGAG CATGATCCTG 
CTCTTTCAGA TGTCGACGTC AGCCGGTTGG GATGGTGTAC TGGACGCCAT TATCAATGAG 
GAAGCATGCG ATCCACCCGA CAACGACAAA GGCTATCCGG GCAATTGTGG TTCAGCGACC 
GTTGGAATAA CGTTTCTCCT CTCATACCTA GTTATAAGCT TTTTGATAGT TATTAATATG 
TACATTGCTG TCATTCTCGA GAACTATAGT CAGGCCACCG AGGACGTGCA AGAGGGTCTA 
ACCGACGACG ACTACGACAT GTACTATGAG ATCTGGCAGC AATTCGATCC GGAGGGCACC 
CAGTACATAC GCTATGATCA GCTGTCCGAA TTCCTGGACG TACTGGAGCC CCCGCTGCAG 
ATCCACAAAC CGAACAAGTA CAAGATCATA TCGATGGACA TACCCATCTG TCGCGGTGAC 
CTCATGTACT GCGTCGACAT CCTCGACGCC CTTACGAAAG ACTTCTTTGC GCGGAAGGGC 
AATCCGATAG AGGAGACGGG TGAGATTGGT GAGATAGCGG CCCGCCCGGA TACGGAGGGC 
TACGAGCCCG TCTCATCAAC GCTGTGGCGT CAGCGTGAGG AGTACTGCGC CCGGCTAATC 
CAGCACGCCT GGCGAAAGCA CAAGGCGCGC GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT 
ACGGATCATG GCGATGGCGG TGATCCGGAT GCCGGGGACC CGGCGCCCGA TGAAGCAACG 
GACGGCGATG CGCCCGCTGG TGGAGATGGT AGTGTTAACG GTACTGCAGA AGGAGCTGCC 
GATGCCGATG AGAGTAATGT AAATAGTCCG GGTGAGGATG CAGCGGCGGC GGCAGCAGCA 
GCAGCAGCAG CGGCGGCGGC GGGCACGACG ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC 
GGGCGACAGA CCGCCGTTCT CGTGGAGAGC GACGGGTTCG TGACGAAGAA CGGCCACAAG 
GTGGTCATCC ACTCGCGATC GCCGAGCATC ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201693.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188622.1 Drosophila melanogaster paralytic (para), transcript variant AA, mRNA.

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CloneID	OFa03643
Clone ID Related Accession (Same CDS sequence)	NM_001201693.1 , NM_001201695.2
Accession Version	NM_001201693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGTCTCC 
GTTTACTATT TCCCAACAGA GGACGATGAC GAGGATGGGC CGACGTTCAA AGACAAGGCA 
CTCGAAGTGA TCCTCAAAGG CATCGATGTG TTTTGTGTGT GGGACTGTTG CTGGGTTTGG 
TTGAAATTTC AGGAGTGGGT ATCGCTCATC GTCTTCGATC CCTTCGTCGA GCTCTTCATC 
ACGCTGTGCA TTGTGGTCAA CACGATGTTC ATGGCAATGG ATCACCACGA TATGAACAAG 
GAGATGGAAC GCGTGCTCAA GAGTGGCAAC TATTTCTTCA CCGCCACCTT TGCCATCGAG 
GCCACCATGA AGCTAATGGC CATGAGCCCC AAGTACTATT TCCAGGAGGG CTGGAACATC 
TTCGACTTCA TTATCGTGGC CCTATCGCTA TTGGAACTGG GACTCGAGGG TGTCCAGGGT 
CTGTCCGTAT TGCGTTCCTT TCGATTGCTG CGTGTATTCA AACTGGCCAA GTCTTGGCCC 
ACACTTAATT TACTCATTTC GATTATGGGA CGCACCATGG GCGCTTTGGG TAATCTGACA 
TTTGTACTTT GCATTATCAT CTTCATCTTT GCGGTGATGG GAATGCAACT GTTCGGAAAG 
AATTATCATG ATCACAAGGA CCGCTTTCCG GATGGCGACC TGCCGCGCTG GAACTTCACC 
GACTTTATGC ACAGCTTCAT GATCGTGTTC CGGGTGCTCT GCGGAGAATG GATCGAGTCC 
ATGTGGGACT GCATGTACGT GGGCGATGTC TCGTGCATTC CCTTCTTCTT GGCCACCGTT 
GTCATCGGCA ATCTTGTGGT ACTTAACCTT TTCTTAGCCT TGCTTTTGTC CAATTTTGGC 
TCATCTAGCT TATCAGCGCC GACTGCCGAT AACGATACGA ATAAAATAGC CGAGGCCTTC 
AATCGAATTG GCCGATTTAA AAGTTGGGTT AAGCGTAATA TTGCTGATTG TTTCAAGTTA 
ATACGTAACA AATTGACAAA TCAAATAAGT GATCAACCAT CAGAGCATGG TGACAACGAA 
CTGGAGCTGG GCCACGACGA GATCCTCGCC GACGGCCTCA TCAAGAAGGG GATCAAGGAG 
CAGACGCAAC TGGAGGTGGC CATCGGGGAT GGCATGGAAT TCACGATACA CGGCGACATG 
AAGAACAACA AGCCGAAGAA ATCCAAATAT CTAAATAACG CAACGGACGA CGACACTGCC 
AGCATTAACT CATATGGTAG CCATAAGAAT CGACCATTCA AGGACGAGAG CCACAAGGGC 
AGCGCCGAGA CGATGGAGGG CGAGGAGAAG CGCGACGCCA GCAAGGAGGA TTTAGGTCTC 
GACGAGGAAC TGGACGAGGA GGGCGAATGC GAGGAGGGCC CGCTCGACGG TGATATCATT 
ATTCATGCAC ACGACGAGGA TATACTCGAT GAATATCCAG CTGATTGCTG CCCCGATTCG 
TACTATAAGA AATTTCCGAT CTTAGCCGGT GACGATGACT CGCCGTTCTG GCAAGGATGG 
GGCAATTTAC GACTGAAAAC TTTTCAATTA ATTGAAAATA AATATTTTGA AACAGCTGTT 
ATCACTATGA TTTTAATGAG TAGCTTAGCT TTGGCATTAG AAGATGTACA TCTGCCACAA 
AGACCCATAC TGCAGGATAT TTTATACTAT ATGGACAGAA TATTTACGGT TATATTCTTC 
TTGGAAATGT TAATCAAGTG GTTGGCGCTC GGCTTCAAAG TGTACTTCAC CAACGCGTGG 
TGTTGGCTCG ATTTCGTGAT TGTCATGGTA TCGCTTATCA ACTTCGTTGC TTCACTTGTT 
GGAGCTGGTG GTATTCAAGC CTTCAAGACT ATGCGAACGT TAAGAGCACT GAGACCACTA 
CGTGCCATGT CCCGTATGCA GGGCATGAGG GTCGTCGTTA ATGCGCTGGT ACAAGCTATA 
CCGTCCATCT TCAATGTGCT ATTGGTGTGT CTAATATTTT GGCTAATTTT TGCCATAATG 
GGTGTACAGC TTTTTGCTGG AAAATATTTT AAGTGCGAGG ACATGAATGG CACGAAGCTC 
AGCCACGAGA TCATACCAAA TCGCAATGCC TGCGAGAGCG AGAACTACAC GTGGGTGAAT 
TCAGCAATGA ATTTCGATCA TGTAGGTAAC GCGTATCTGT GCCTTTTCCA AGTGGCCACC 
TTCAAAGGCT GGATACAAAT CATGAACGAT GCTATCGATT CACGAGAGGT GGACAAGCAA 
CCAATTCGTG AAACGAACAT CTACATGTAT TTATATTTCG TATTCTTCAT CATATTTGGA 
TCATTTTTCA CACTCAATCT GTTCATTGGT GTTATCATTG ATAATTTTAA TGAGCAAAAG 
AAAAAAGCAG GTGGATCATT AGAAATGTTC ATGACAGAAG ATCAGAAAAA GTACTATAAT 
GCTATGAAAA AGATGGGCTC TAAAAAACCA TTAAAAGCCA TTCCAAGACC AAGGTGGCGA 
CCACAAGCAA TAGTCTTTGA AATAGTAACC GATAAGAAAT TCGATATAAT CATTATGTTA 
TTCATTGGTC TGAACATGTT CACCATGACC CTCGATCGTT ACGATGCGTC GGACACGTAT 
AACGCGGTCC TAGACTATCT CAATGCGATA TTCGTAGTTA TTTTCAGTTC CGAATGTCTA 
TTAAAAATAT TCGCTTTACG ATATCACTAT TTTATTGAGC CATGGAATTT ATTTGATGTA 
GTAGTTGTCA TTTTATCCAT CTTAGGTCTT GTACTTAGCG ATATTATCGA GAAGTACTTC 
GTGTCGCCGA CCCTGCTCCG AGTGGTGCGT GTGGCGAAAG TGGGCCGTGT CCTTCGACTG 
GTGAAGGGAG CCAAGGGCAT TCGGACACTG CTCTTCGCGT TGGCCATGTC GCTGCCGGCC 
CTGTTCAACA TCTGCCTGCT GCTGTTCCTG GTCATGTTCA TCTTTGCCAT TTTCGGCATG 
TCGTTCTTCA TGCACGTGAA GGAGAAGAGC GGCATCAACG ACGTCTACAA CTTCAAGACC 
TTTGGCCAGA GCATGATCCT GCTCTTTCAG ATGTCGACGT CAGCCGGTTG GGATGGTGTA 
CTGGACGCCA TTATCAATGA GGAAGCATGC GATCCACCCG ACAACGACAA AGGCTATCCG 
GGCAATTGTG GTTCAGCGAC CGTTGGAATA ACGTTTCTCC TCTCATACCT AGTTATAAGC 
TTTTTGATAG TTATTAATAT GTACATTGCT GTCATTCTCG AGAACTATAG TCAGGCCACC 
GAGGACGTGC AAGAGGGTCT AACCGACGAC GACTACGACA TGTACTATGA GATCTGGCAG 
CAATTCGATC CGGAGGGCAC CCAGTACATA CGCTATGATC AGCTGTCCGA ATTCCTGGAC 
GTACTGGAGC CCCCGCTGCA GATCCACAAA CCGAACAAGT ACAAGATCAT ATCGATGGAC 
ATACCCATCT GTCGCGGTGA CCTCATGTAC TGCGTCGACA TCCTCGACGC CCTTACGAAA 
GACTTCTTTG CGCGGAAGGG CAATCCGATA GAGGAGACGG GTGAGATTGG TGAGATAGCG 
GCCCGCCCGG ATACGGAGGG CTACGAGCCC GTCTCATCAA CGCTGTGGCG TCAGCGTGAG 
GAGTACTGCG CCCGGCTAAT CCAGCACGCC TGGCGAAAGC ACAAGGCGCG CGGCGAGGGA 
GGTGGGTCCT TTGAGCCGGA TACGGATCAT GGCGATGGCG GTGATCCGGA TGCCGGGGAC 
CCGGCGCCCG ATGAAGCAAC GGACGGCGAT GCGCCCGCTG GTGGAGATGG TAGTGTTAAC 
GGTACTGCAG AAGGAGCTGC CGATGCCGAT GAGAGTAATG TAAATAGTCC GGGTGAGGAT 
GCAGCGGCGG CGGCAGCAGC AGCAGCAGCA GCGGCGGCGG CGGGCACGAC GACGGCGGGA 
AGTCCCGGAG CGGGTAGCGC CGGGCGACAG ACCGCCGTTC TCGTGGAGAG CGACGGGTTC 
GTGACGAAGA ACGGCCACAA GGTGGTCATC CACTCGCGAT CGCCGAGCAT CACGTCGCGC 
ACGGCGGATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188622.1
CDS	269..6523
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201693.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AA, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201693.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGTCTCC 
GTTTACTATT TCCCAACAGA GGACGATGAC GAGGATGGGC CGACGTTCAA AGACAAGGCA 
CTCGAAGTGA TCCTCAAAGG CATCGATGTG TTTTGTGTGT GGGACTGTTG CTGGGTTTGG 
TTGAAATTTC AGGAGTGGGT ATCGCTCATC GTCTTCGATC CCTTCGTCGA GCTCTTCATC 
ACGCTGTGCA TTGTGGTCAA CACGATGTTC ATGGCAATGG ATCACCACGA TATGAACAAG 
GAGATGGAAC GCGTGCTCAA GAGTGGCAAC TATTTCTTCA CCGCCACCTT TGCCATCGAG 
GCCACCATGA AGCTAATGGC CATGAGCCCC AAGTACTATT TCCAGGAGGG CTGGAACATC 
TTCGACTTCA TTATCGTGGC CCTATCGCTA TTGGAACTGG GACTCGAGGG TGTCCAGGGT 
CTGTCCGTAT TGCGTTCCTT TCGATTGCTG CGTGTATTCA AACTGGCCAA GTCTTGGCCC 
ACACTTAATT TACTCATTTC GATTATGGGA CGCACCATGG GCGCTTTGGG TAATCTGACA 
TTTGTACTTT GCATTATCAT CTTCATCTTT GCGGTGATGG GAATGCAACT GTTCGGAAAG 
AATTATCATG ATCACAAGGA CCGCTTTCCG GATGGCGACC TGCCGCGCTG GAACTTCACC 
GACTTTATGC ACAGCTTCAT GATCGTGTTC CGGGTGCTCT GCGGAGAATG GATCGAGTCC 
ATGTGGGACT GCATGTACGT GGGCGATGTC TCGTGCATTC CCTTCTTCTT GGCCACCGTT 
GTCATCGGCA ATCTTGTGGT ACTTAACCTT TTCTTAGCCT TGCTTTTGTC CAATTTTGGC 
TCATCTAGCT TATCAGCGCC GACTGCCGAT AACGATACGA ATAAAATAGC CGAGGCCTTC 
AATCGAATTG GCCGATTTAA AAGTTGGGTT AAGCGTAATA TTGCTGATTG TTTCAAGTTA 
ATACGTAACA AATTGACAAA TCAAATAAGT GATCAACCAT CAGAGCATGG TGACAACGAA 
CTGGAGCTGG GCCACGACGA GATCCTCGCC GACGGCCTCA TCAAGAAGGG GATCAAGGAG 
CAGACGCAAC TGGAGGTGGC CATCGGGGAT GGCATGGAAT TCACGATACA CGGCGACATG 
AAGAACAACA AGCCGAAGAA ATCCAAATAT CTAAATAACG CAACGGACGA CGACACTGCC 
AGCATTAACT CATATGGTAG CCATAAGAAT CGACCATTCA AGGACGAGAG CCACAAGGGC 
AGCGCCGAGA CGATGGAGGG CGAGGAGAAG CGCGACGCCA GCAAGGAGGA TTTAGGTCTC 
GACGAGGAAC TGGACGAGGA GGGCGAATGC GAGGAGGGCC CGCTCGACGG TGATATCATT 
ATTCATGCAC ACGACGAGGA TATACTCGAT GAATATCCAG CTGATTGCTG CCCCGATTCG 
TACTATAAGA AATTTCCGAT CTTAGCCGGT GACGATGACT CGCCGTTCTG GCAAGGATGG 
GGCAATTTAC GACTGAAAAC TTTTCAATTA ATTGAAAATA AATATTTTGA AACAGCTGTT 
ATCACTATGA TTTTAATGAG TAGCTTAGCT TTGGCATTAG AAGATGTACA TCTGCCACAA 
AGACCCATAC TGCAGGATAT TTTATACTAT ATGGACAGAA TATTTACGGT TATATTCTTC 
TTGGAAATGT TAATCAAGTG GTTGGCGCTC GGCTTCAAAG TGTACTTCAC CAACGCGTGG 
TGTTGGCTCG ATTTCGTGAT TGTCATGGTA TCGCTTATCA ACTTCGTTGC TTCACTTGTT 
GGAGCTGGTG GTATTCAAGC CTTCAAGACT ATGCGAACGT TAAGAGCACT GAGACCACTA 
CGTGCCATGT CCCGTATGCA GGGCATGAGG GTCGTCGTTA ATGCGCTGGT ACAAGCTATA 
CCGTCCATCT TCAATGTGCT ATTGGTGTGT CTAATATTTT GGCTAATTTT TGCCATAATG 
GGTGTACAGC TTTTTGCTGG AAAATATTTT AAGTGCGAGG ACATGAATGG CACGAAGCTC 
AGCCACGAGA TCATACCAAA TCGCAATGCC TGCGAGAGCG AGAACTACAC GTGGGTGAAT 
TCAGCAATGA ATTTCGATCA TGTAGGTAAC GCGTATCTGT GCCTTTTCCA AGTGGCCACC 
TTCAAAGGCT GGATACAAAT CATGAACGAT GCTATCGATT CACGAGAGGT GGACAAGCAA 
CCAATTCGTG AAACGAACAT CTACATGTAT TTATATTTCG TATTCTTCAT CATATTTGGA 
TCATTTTTCA CACTCAATCT GTTCATTGGT GTTATCATTG ATAATTTTAA TGAGCAAAAG 
AAAAAAGCAG GTGGATCATT AGAAATGTTC ATGACAGAAG ATCAGAAAAA GTACTATAAT 
GCTATGAAAA AGATGGGCTC TAAAAAACCA TTAAAAGCCA TTCCAAGACC AAGGTGGCGA 
CCACAAGCAA TAGTCTTTGA AATAGTAACC GATAAGAAAT TCGATATAAT CATTATGTTA 
TTCATTGGTC TGAACATGTT CACCATGACC CTCGATCGTT ACGATGCGTC GGACACGTAT 
AACGCGGTCC TAGACTATCT CAATGCGATA TTCGTAGTTA TTTTCAGTTC CGAATGTCTA 
TTAAAAATAT TCGCTTTACG ATATCACTAT TTTATTGAGC CATGGAATTT ATTTGATGTA 
GTAGTTGTCA TTTTATCCAT CTTAGGTCTT GTACTTAGCG ATATTATCGA GAAGTACTTC 
GTGTCGCCGA CCCTGCTCCG AGTGGTGCGT GTGGCGAAAG TGGGCCGTGT CCTTCGACTG 
GTGAAGGGAG CCAAGGGCAT TCGGACACTG CTCTTCGCGT TGGCCATGTC GCTGCCGGCC 
CTGTTCAACA TCTGCCTGCT GCTGTTCCTG GTCATGTTCA TCTTTGCCAT TTTCGGCATG 
TCGTTCTTCA TGCACGTGAA GGAGAAGAGC GGCATCAACG ACGTCTACAA CTTCAAGACC 
TTTGGCCAGA GCATGATCCT GCTCTTTCAG ATGTCGACGT CAGCCGGTTG GGATGGTGTA 
CTGGACGCCA TTATCAATGA GGAAGCATGC GATCCACCCG ACAACGACAA AGGCTATCCG 
GGCAATTGTG GTTCAGCGAC CGTTGGAATA ACGTTTCTCC TCTCATACCT AGTTATAAGC 
TTTTTGATAG TTATTAATAT GTACATTGCT GTCATTCTCG AGAACTATAG TCAGGCCACC 
GAGGACGTGC AAGAGGGTCT AACCGACGAC GACTACGACA TGTACTATGA GATCTGGCAG 
CAATTCGATC CGGAGGGCAC CCAGTACATA CGCTATGATC AGCTGTCCGA ATTCCTGGAC 
GTACTGGAGC CCCCGCTGCA GATCCACAAA CCGAACAAGT ACAAGATCAT ATCGATGGAC 
ATACCCATCT GTCGCGGTGA CCTCATGTAC TGCGTCGACA TCCTCGACGC CCTTACGAAA 
GACTTCTTTG CGCGGAAGGG CAATCCGATA GAGGAGACGG GTGAGATTGG TGAGATAGCG 
GCCCGCCCGG ATACGGAGGG CTACGAGCCC GTCTCATCAA CGCTGTGGCG TCAGCGTGAG 
GAGTACTGCG CCCGGCTAAT CCAGCACGCC TGGCGAAAGC ACAAGGCGCG CGGCGAGGGA 
GGTGGGTCCT TTGAGCCGGA TACGGATCAT GGCGATGGCG GTGATCCGGA TGCCGGGGAC 
CCGGCGCCCG ATGAAGCAAC GGACGGCGAT GCGCCCGCTG GTGGAGATGG TAGTGTTAAC 
GGTACTGCAG AAGGAGCTGC CGATGCCGAT GAGAGTAATG TAAATAGTCC GGGTGAGGAT 
GCAGCGGCGG CGGCAGCAGC AGCAGCAGCA GCGGCGGCGG CGGGCACGAC GACGGCGGGA 
AGTCCCGGAG CGGGTAGCGC CGGGCGACAG ACCGCCGTTC TCGTGGAGAG CGACGGGTTC 
GTGACGAAGA ACGGCCACAA GGTGGTCATC CACTCGCGAT CGCCGAGCAT CACGTCGCGC 
ACGGCGGATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201695.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188624.1 Drosophila melanogaster paralytic (para), transcript variant AC, mRNA.

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CloneID	OFa03643
Clone ID Related Accession (Same CDS sequence)	NM_001201693.1 , NM_001201695.2
Accession Version	NM_001201695.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AC
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_001201695.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGTCTCC 
GTTTACTATT TCCCAACAGA GGACGATGAC GAGGATGGGC CGACGTTCAA AGACAAGGCA 
CTCGAAGTGA TCCTCAAAGG CATCGATGTG TTTTGTGTGT GGGACTGTTG CTGGGTTTGG 
TTGAAATTTC AGGAGTGGGT ATCGCTCATC GTCTTCGATC CCTTCGTCGA GCTCTTCATC 
ACGCTGTGCA TTGTGGTCAA CACGATGTTC ATGGCAATGG ATCACCACGA TATGAACAAG 
GAGATGGAAC GCGTGCTCAA GAGTGGCAAC TATTTCTTCA CCGCCACCTT TGCCATCGAG 
GCCACCATGA AGCTAATGGC CATGAGCCCC AAGTACTATT TCCAGGAGGG CTGGAACATC 
TTCGACTTCA TTATCGTGGC CCTATCGCTA TTGGAACTGG GACTCGAGGG TGTCCAGGGT 
CTGTCCGTAT TGCGTTCCTT TCGATTGCTG CGTGTATTCA AACTGGCCAA GTCTTGGCCC 
ACACTTAATT TACTCATTTC GATTATGGGA CGCACCATGG GCGCTTTGGG TAATCTGACA 
TTTGTACTTT GCATTATCAT CTTCATCTTT GCGGTGATGG GAATGCAACT GTTCGGAAAG 
AATTATCATG ATCACAAGGA CCGCTTTCCG GATGGCGACC TGCCGCGCTG GAACTTCACC 
GACTTTATGC ACAGCTTCAT GATCGTGTTC CGGGTGCTCT GCGGAGAATG GATCGAGTCC 
ATGTGGGACT GCATGTACGT GGGCGATGTC TCGTGCATTC CCTTCTTCTT GGCCACCGTT 
GTCATCGGCA ATCTTGTGGT ACTTAACCTT TTCTTAGCCT TGCTTTTGTC CAATTTTGGC 
TCATCTAGCT TATCAGCGCC GACTGCCGAT AACGATACGA ATAAAATAGC CGAGGCCTTC 
AATCGAATTG GCCGATTTAA AAGTTGGGTT AAGCGTAATA TTGCTGATTG TTTCAAGTTA 
ATACGTAACA AATTGACAAA TCAAATAAGT GATCAACCAT CAGAGCATGG TGACAACGAA 
CTGGAGCTGG GCCACGACGA GATCCTCGCC GACGGCCTCA TCAAGAAGGG GATCAAGGAG 
CAGACGCAAC TGGAGGTGGC CATCGGGGAT GGCATGGAAT TCACGATACA CGGCGACATG 
AAGAACAACA AGCCGAAGAA ATCCAAATAT CTAAATAACG CAACGGACGA CGACACTGCC 
AGCATTAACT CATATGGTAG CCATAAGAAT CGACCATTCA AGGACGAGAG CCACAAGGGC 
AGCGCCGAGA CGATGGAGGG CGAGGAGAAG CGCGACGCCA GCAAGGAGGA TTTAGGTCTC 
GACGAGGAAC TGGACGAGGA GGGCGAATGC GAGGAGGGCC CGCTCGACGG TGATATCATT 
ATTCATGCAC ACGACGAGGA TATACTCGAT GAATATCCAG CTGATTGCTG CCCCGATTCG 
TACTATAAGA AATTTCCGAT CTTAGCCGGT GACGATGACT CGCCGTTCTG GCAAGGATGG 
GGCAATTTAC GACTGAAAAC TTTTCAATTA ATTGAAAATA AATATTTTGA AACAGCTGTT 
ATCACTATGA TTTTAATGAG TAGCTTAGCT TTGGCATTAG AAGATGTACA TCTGCCACAA 
AGACCCATAC TGCAGGATAT TTTATACTAT ATGGACAGAA TATTTACGGT TATATTCTTC 
TTGGAAATGT TAATCAAGTG GTTGGCGCTC GGCTTCAAAG TGTACTTCAC CAACGCGTGG 
TGTTGGCTCG ATTTCGTGAT TGTCATGGTA TCGCTTATCA ACTTCGTTGC TTCACTTGTT 
GGAGCTGGTG GTATTCAAGC CTTCAAGACT ATGCGAACGT TAAGAGCACT GAGACCACTA 
CGTGCCATGT CCCGTATGCA GGGCATGAGG GTCGTCGTTA ATGCGCTGGT ACAAGCTATA 
CCGTCCATCT TCAATGTGCT ATTGGTGTGT CTAATATTTT GGCTAATTTT TGCCATAATG 
GGTGTACAGC TTTTTGCTGG AAAATATTTT AAGTGCGAGG ACATGAATGG CACGAAGCTC 
AGCCACGAGA TCATACCAAA TCGCAATGCC TGCGAGAGCG AGAACTACAC GTGGGTGAAT 
TCAGCAATGA ATTTCGATCA TGTAGGTAAC GCGTATCTGT GCCTTTTCCA AGTGGCCACC 
TTCAAAGGCT GGATACAAAT CATGAACGAT GCTATCGATT CACGAGAGGT GGACAAGCAA 
CCAATTCGTG AAACGAACAT CTACATGTAT TTATATTTCG TATTCTTCAT CATATTTGGA 
TCATTTTTCA CACTCAATCT GTTCATTGGT GTTATCATTG ATAATTTTAA TGAGCAAAAG 
AAAAAAGCAG GTGGATCATT AGAAATGTTC ATGACAGAAG ATCAGAAAAA GTACTATAAT 
GCTATGAAAA AGATGGGCTC TAAAAAACCA TTAAAAGCCA TTCCAAGACC AAGGTGGCGA 
CCACAAGCAA TAGTCTTTGA AATAGTAACC GATAAGAAAT TCGATATAAT CATTATGTTA 
TTCATTGGTC TGAACATGTT CACCATGACC CTCGATCGTT ACGATGCGTC GGACACGTAT 
AACGCGGTCC TAGACTATCT CAATGCGATA TTCGTAGTTA TTTTCAGTTC CGAATGTCTA 
TTAAAAATAT TCGCTTTACG ATATCACTAT TTTATTGAGC CATGGAATTT ATTTGATGTA 
GTAGTTGTCA TTTTATCCAT CTTAGGTCTT GTACTTAGCG ATATTATCGA GAAGTACTTC 
GTGTCGCCGA CCCTGCTCCG AGTGGTGCGT GTGGCGAAAG TGGGCCGTGT CCTTCGACTG 
GTGAAGGGAG CCAAGGGCAT TCGGACACTG CTCTTCGCGT TGGCCATGTC GCTGCCGGCC 
CTGTTCAACA TCTGCCTGCT GCTGTTCCTG GTCATGTTCA TCTTTGCCAT TTTCGGCATG 
TCGTTCTTCA TGCACGTGAA GGAGAAGAGC GGCATCAACG ACGTCTACAA CTTCAAGACC 
TTTGGCCAGA GCATGATCCT GCTCTTTCAG ATGTCGACGT CAGCCGGTTG GGATGGTGTA 
CTGGACGCCA TTATCAATGA GGAAGCATGC GATCCACCCG ACAACGACAA AGGCTATCCG 
GGCAATTGTG GTTCAGCGAC CGTTGGAATA ACGTTTCTCC TCTCATACCT AGTTATAAGC 
TTTTTGATAG TTATTAATAT GTACATTGCT GTCATTCTCG AGAACTATAG TCAGGCCACC 
GAGGACGTGC AAGAGGGTCT AACCGACGAC GACTACGACA TGTACTATGA GATCTGGCAG 
CAATTCGATC CGGAGGGCAC CCAGTACATA CGCTATGATC AGCTGTCCGA ATTCCTGGAC 
GTACTGGAGC CCCCGCTGCA GATCCACAAA CCGAACAAGT ACAAGATCAT ATCGATGGAC 
ATACCCATCT GTCGCGGTGA CCTCATGTAC TGCGTCGACA TCCTCGACGC CCTTACGAAA 
GACTTCTTTG CGCGGAAGGG CAATCCGATA GAGGAGACGG GTGAGATTGG TGAGATAGCG 
GCCCGCCCGG ATACGGAGGG CTACGAGCCC GTCTCATCAA CGCTGTGGCG TCAGCGTGAG 
GAGTACTGCG CCCGGCTAAT CCAGCACGCC TGGCGAAAGC ACAAGGCGCG CGGCGAGGGA 
GGTGGGTCCT TTGAGCCGGA TACGGATCAT GGCGATGGCG GTGATCCGGA TGCCGGGGAC 
CCGGCGCCCG ATGAAGCAAC GGACGGCGAT GCGCCCGCTG GTGGAGATGG TAGTGTTAAC 
GGTACTGCAG AAGGAGCTGC CGATGCCGAT GAGAGTAATG TAAATAGTCC GGGTGAGGAT 
GCAGCGGCGG CGGCAGCAGC AGCAGCAGCA GCGGCGGCGG CGGGCACGAC GACGGCGGGA 
AGTCCCGGAG CGGGTAGCGC CGGGCGACAG ACCGCCGTTC TCGTGGAGAG CGACGGGTTC 
GTGACGAAGA ACGGCCACAA GGTGGTCATC CACTCGCGAT CGCCGAGCAT CACGTCGCGC 
ACGGCGGATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188624.1
CDS	269..6523
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201695.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AC, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201695.2

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGTCTCC 
GTTTACTATT TCCCAACAGA GGACGATGAC GAGGATGGGC CGACGTTCAA AGACAAGGCA 
CTCGAAGTGA TCCTCAAAGG CATCGATGTG TTTTGTGTGT GGGACTGTTG CTGGGTTTGG 
TTGAAATTTC AGGAGTGGGT ATCGCTCATC GTCTTCGATC CCTTCGTCGA GCTCTTCATC 
ACGCTGTGCA TTGTGGTCAA CACGATGTTC ATGGCAATGG ATCACCACGA TATGAACAAG 
GAGATGGAAC GCGTGCTCAA GAGTGGCAAC TATTTCTTCA CCGCCACCTT TGCCATCGAG 
GCCACCATGA AGCTAATGGC CATGAGCCCC AAGTACTATT TCCAGGAGGG CTGGAACATC 
TTCGACTTCA TTATCGTGGC CCTATCGCTA TTGGAACTGG GACTCGAGGG TGTCCAGGGT 
CTGTCCGTAT TGCGTTCCTT TCGATTGCTG CGTGTATTCA AACTGGCCAA GTCTTGGCCC 
ACACTTAATT TACTCATTTC GATTATGGGA CGCACCATGG GCGCTTTGGG TAATCTGACA 
TTTGTACTTT GCATTATCAT CTTCATCTTT GCGGTGATGG GAATGCAACT GTTCGGAAAG 
AATTATCATG ATCACAAGGA CCGCTTTCCG GATGGCGACC TGCCGCGCTG GAACTTCACC 
GACTTTATGC ACAGCTTCAT GATCGTGTTC CGGGTGCTCT GCGGAGAATG GATCGAGTCC 
ATGTGGGACT GCATGTACGT GGGCGATGTC TCGTGCATTC CCTTCTTCTT GGCCACCGTT 
GTCATCGGCA ATCTTGTGGT ACTTAACCTT TTCTTAGCCT TGCTTTTGTC CAATTTTGGC 
TCATCTAGCT TATCAGCGCC GACTGCCGAT AACGATACGA ATAAAATAGC CGAGGCCTTC 
AATCGAATTG GCCGATTTAA AAGTTGGGTT AAGCGTAATA TTGCTGATTG TTTCAAGTTA 
ATACGTAACA AATTGACAAA TCAAATAAGT GATCAACCAT CAGAGCATGG TGACAACGAA 
CTGGAGCTGG GCCACGACGA GATCCTCGCC GACGGCCTCA TCAAGAAGGG GATCAAGGAG 
CAGACGCAAC TGGAGGTGGC CATCGGGGAT GGCATGGAAT TCACGATACA CGGCGACATG 
AAGAACAACA AGCCGAAGAA ATCCAAATAT CTAAATAACG CAACGGACGA CGACACTGCC 
AGCATTAACT CATATGGTAG CCATAAGAAT CGACCATTCA AGGACGAGAG CCACAAGGGC 
AGCGCCGAGA CGATGGAGGG CGAGGAGAAG CGCGACGCCA GCAAGGAGGA TTTAGGTCTC 
GACGAGGAAC TGGACGAGGA GGGCGAATGC GAGGAGGGCC CGCTCGACGG TGATATCATT 
ATTCATGCAC ACGACGAGGA TATACTCGAT GAATATCCAG CTGATTGCTG CCCCGATTCG 
TACTATAAGA AATTTCCGAT CTTAGCCGGT GACGATGACT CGCCGTTCTG GCAAGGATGG 
GGCAATTTAC GACTGAAAAC TTTTCAATTA ATTGAAAATA AATATTTTGA AACAGCTGTT 
ATCACTATGA TTTTAATGAG TAGCTTAGCT TTGGCATTAG AAGATGTACA TCTGCCACAA 
AGACCCATAC TGCAGGATAT TTTATACTAT ATGGACAGAA TATTTACGGT TATATTCTTC 
TTGGAAATGT TAATCAAGTG GTTGGCGCTC GGCTTCAAAG TGTACTTCAC CAACGCGTGG 
TGTTGGCTCG ATTTCGTGAT TGTCATGGTA TCGCTTATCA ACTTCGTTGC TTCACTTGTT 
GGAGCTGGTG GTATTCAAGC CTTCAAGACT ATGCGAACGT TAAGAGCACT GAGACCACTA 
CGTGCCATGT CCCGTATGCA GGGCATGAGG GTCGTCGTTA ATGCGCTGGT ACAAGCTATA 
CCGTCCATCT TCAATGTGCT ATTGGTGTGT CTAATATTTT GGCTAATTTT TGCCATAATG 
GGTGTACAGC TTTTTGCTGG AAAATATTTT AAGTGCGAGG ACATGAATGG CACGAAGCTC 
AGCCACGAGA TCATACCAAA TCGCAATGCC TGCGAGAGCG AGAACTACAC GTGGGTGAAT 
TCAGCAATGA ATTTCGATCA TGTAGGTAAC GCGTATCTGT GCCTTTTCCA AGTGGCCACC 
TTCAAAGGCT GGATACAAAT CATGAACGAT GCTATCGATT CACGAGAGGT GGACAAGCAA 
CCAATTCGTG AAACGAACAT CTACATGTAT TTATATTTCG TATTCTTCAT CATATTTGGA 
TCATTTTTCA CACTCAATCT GTTCATTGGT GTTATCATTG ATAATTTTAA TGAGCAAAAG 
AAAAAAGCAG GTGGATCATT AGAAATGTTC ATGACAGAAG ATCAGAAAAA GTACTATAAT 
GCTATGAAAA AGATGGGCTC TAAAAAACCA TTAAAAGCCA TTCCAAGACC AAGGTGGCGA 
CCACAAGCAA TAGTCTTTGA AATAGTAACC GATAAGAAAT TCGATATAAT CATTATGTTA 
TTCATTGGTC TGAACATGTT CACCATGACC CTCGATCGTT ACGATGCGTC GGACACGTAT 
AACGCGGTCC TAGACTATCT CAATGCGATA TTCGTAGTTA TTTTCAGTTC CGAATGTCTA 
TTAAAAATAT TCGCTTTACG ATATCACTAT TTTATTGAGC CATGGAATTT ATTTGATGTA 
GTAGTTGTCA TTTTATCCAT CTTAGGTCTT GTACTTAGCG ATATTATCGA GAAGTACTTC 
GTGTCGCCGA CCCTGCTCCG AGTGGTGCGT GTGGCGAAAG TGGGCCGTGT CCTTCGACTG 
GTGAAGGGAG CCAAGGGCAT TCGGACACTG CTCTTCGCGT TGGCCATGTC GCTGCCGGCC 
CTGTTCAACA TCTGCCTGCT GCTGTTCCTG GTCATGTTCA TCTTTGCCAT TTTCGGCATG 
TCGTTCTTCA TGCACGTGAA GGAGAAGAGC GGCATCAACG ACGTCTACAA CTTCAAGACC 
TTTGGCCAGA GCATGATCCT GCTCTTTCAG ATGTCGACGT CAGCCGGTTG GGATGGTGTA 
CTGGACGCCA TTATCAATGA GGAAGCATGC GATCCACCCG ACAACGACAA AGGCTATCCG 
GGCAATTGTG GTTCAGCGAC CGTTGGAATA ACGTTTCTCC TCTCATACCT AGTTATAAGC 
TTTTTGATAG TTATTAATAT GTACATTGCT GTCATTCTCG AGAACTATAG TCAGGCCACC 
GAGGACGTGC AAGAGGGTCT AACCGACGAC GACTACGACA TGTACTATGA GATCTGGCAG 
CAATTCGATC CGGAGGGCAC CCAGTACATA CGCTATGATC AGCTGTCCGA ATTCCTGGAC 
GTACTGGAGC CCCCGCTGCA GATCCACAAA CCGAACAAGT ACAAGATCAT ATCGATGGAC 
ATACCCATCT GTCGCGGTGA CCTCATGTAC TGCGTCGACA TCCTCGACGC CCTTACGAAA 
GACTTCTTTG CGCGGAAGGG CAATCCGATA GAGGAGACGG GTGAGATTGG TGAGATAGCG 
GCCCGCCCGG ATACGGAGGG CTACGAGCCC GTCTCATCAA CGCTGTGGCG TCAGCGTGAG 
GAGTACTGCG CCCGGCTAAT CCAGCACGCC TGGCGAAAGC ACAAGGCGCG CGGCGAGGGA 
GGTGGGTCCT TTGAGCCGGA TACGGATCAT GGCGATGGCG GTGATCCGGA TGCCGGGGAC 
CCGGCGCCCG ATGAAGCAAC GGACGGCGAT GCGCCCGCTG GTGGAGATGG TAGTGTTAAC 
GGTACTGCAG AAGGAGCTGC CGATGCCGAT GAGAGTAATG TAAATAGTCC GGGTGAGGAT 
GCAGCGGCGG CGGCAGCAGC AGCAGCAGCA GCGGCGGCGG CGGGCACGAC GACGGCGGGA 
AGTCCCGGAG CGGGTAGCGC CGGGCGACAG ACCGCCGTTC TCGTGGAGAG CGACGGGTTC 
GTGACGAAGA ACGGCCACAA GGTGGTCATC CACTCGCGAT CGCCGAGCAT CACGTCGCGC 
ACGGCGGATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201712.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188641.1 Drosophila melanogaster paralytic (para), transcript variant AT, mRNA.

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CloneID	OFa03647
Clone ID Related Accession (Same CDS sequence)	NM_001201712.1
Accession Version	NM_001201712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AT
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GATTATGTGA CCATGGGTAT AGATTTAGGT 
AATCTAGCAG CCCTGCGAAC GTTTAGGGTG CTGCGAGCGC TTAAAACCGT AGCCATTGTG 
CCAGGCTTGA AGACCATCGT CGGCGCCGTC ATCGAATCGG TGAAGAATCT GCGCGATGTG 
ATTATCCTGA CCATGTTCTC CCTGTCGGTG TTCGCGTTGA TGGGCCTACA GATCTATATG 
GGCGTGCTCA CGCAGAAGTG CATCAAGAAG TTCCCGCTGG ACGGTTCCTG GGGCAATCTG 
ACCGACGAGA ACTGGGACTA TCACAATCGC AATAGCTCCA ATTGGTATTC CGAGGACGAG 
GGCATCTCAT TTCCGTTATG CGGCAATATA TCCGGTGCGG GGCAATGCGA CGACGATTAC 
GTGTGCCTGC AGGGGTTTGG TCCGAATCCG AATTATGGCT ACACCAGCTT CGATTCGTTC 
GGATGGGCTT TCCTGTCCGC CTTCCGGCTG ATGACACAGG ACTTCTGGGA GGATCTGTAC 
CAGCTGGTGT TGCGCGCCGC CGGACCATGG CACATGCTGT TCTTTATAGT CATCATCTTC 
CTAGGTTCAT TCTATCTTGT GAATTTGATT TTGGCCATTG TTGCCATGTC GTATGACGAA 
TTGCAAAAGA AGGCCGAAGA AGAAGAGGCT GCCGAAGAGG AGGCGATACG TGAAGCGGAA 
GAAGCTGCCG CCGCCAAAGC GGCCAAGCTG GAGGAGCGGG CCAATGCGCA GGCTCAGGCA 
GCAGCGGATG CGGCTGCCGC CGAAGAGGCT GCACTGCATC CGGAAATGGC CAAGAGTCCG 
ACGTATTCTT GCATCAGCTA TGAGCTATTT GTTGGCGGCG AGAAGGGCAA CGATGACAAC 
AACAAAGAGA AGATGTCCAT TCGGAGCGTC GAGGTGGAGT CGGAGTCGGT GAGCGTTATA 
CAAAGACAAC CAGCACCTAC CACAGCACAC CAAGCTACCA AAGTTCGTAA AGTGAGCACG 
TACACGATAC GGAACGGACG TGGCCGCTTT GGTATACCCG GTAGCGATCG TAAGCCATTG 
GTATTGTCAA CATATCAGGA TGCCCAGCAG CACTTGCCCT ATGCCGACGA CTCGAATGCC 
GTCACCCCGA TGTCCGAAGA GAATGGGGCC ATCATAGTGC CCGTGTACTA TGGCAATCTA 
GGCTCCCGAC ACTCATCGTA TACCTCGCAT CAGTCCCGAA TATCGTATAC CTCACATGGC 
GATCTACTCG GCGGCATGGC CGTCATGGGC GTCAGCACAA TGACCAAGGA GAGCAAATTG 
CGCAACCGGA ACACACGCAA TCAATCAGTG GGCGCCACCA ATGGCGGCAC CACCTGTCTG 
GACACCAATC ACAAGCTCGA TCATCGCGAC TACGAAATTG GCCTGGAGTG CACGGACGAA 
GCTGGCAAGA TTAAACATCA TGACAATCCT TTTATCGAGC CCGTCCAGAC ACAAACGGTG 
GTTGATATGA AAGATGTGAT GGTCCTGAAT GACATCATCG AACAGGCCGC TGGTCGGCAC 
AGTCGGGCAA GCGATCGCGG TGTCTCCGTT TACTATTTCC CAACAGAGGA CGATGACGAG 
GATGGGCCGA CGTTCAAAGA CAAGGCACTC GAAGTGATCC TCAAAGGCAT CGATGTGTTT 
TGTGTGTGGG ACTGTTGCTG GGTTTGGTTG AAATTTCAGG AGTGGGTATC GCTCATCGTC 
TTCGATCCCT TCGTCGAGCT CTTCATCACG CTGTGCATTG TGGTCAACAC GATGTTCATG 
GCAATGGATC ACCACGATAT GAACAAGGAG ATGGAACGCG TGCTCAAGAG TGGCAACTAT 
TTCTTCACCG CCACCTTTGC CATCGAGGCC ACCATGAAGC TAATGGCCAT GAGCCCCAAG 
TACTATTTCC AGGAGGGCTG GAACATCTTC GACTTCATTA TCGTGGCCCT ATCGCTATTG 
GAACTGGGAC TCGAGGGTGT CCAGGGTCTG TCCGTATTGC GTTCCTTTCG ATTGCTGCGT 
GTATTCAAAC TGGCCAAGTC TTGGCCCACA CTTAATTTAC TCATTTCGAT TATGGGACGC 
ACCATGGGCG CTTTGGGTAA TCTGACATTT GTACTTTGCA TTATCATCTT CATCTTTGCG 
GTGATGGGAA TGCAACTGTT CGGAAAGAAT TATCATGATC ACAAGGACCG CTTTCCGGAT 
GGCGACCTGC CGCGCTGGAA CTTCACCGAC TTTATGCACA GCTTCATGAT CGTGTTCCGG 
GTGCTCTGCG GAGAATGGAT CGAGTCCATG TGGGACTGCA TGTACGTGGG CGATGTCTCG 
TGCATTCCCT TCTTCTTGGC CACCGTTGTC ATCGGCAATC TTGTGGTACT TAACCTTTTC 
TTAGCCTTGC TTTTGTCCAA TTTTGGCTCA TCTAGCTTAT CAGCGCCGAC TGCCGATAAC 
GATACGAATA AAATAGCCGA GGCCTTCAAT CGAATTGGCC GATTTAAAAG TTGGGTTAAG 
CGTAATATTG CTGATTGTTT CAAGTTAATA CGTAACAAAT TGACAAATCA AATAAGTGAT 
CAACCATCAG GTGAGAGGAC CAACCAGATC AGTTGGATTT GGAGCGAAGG CAAAGGCGTT 
TGTCGTTGTA TATCTGCAGA GCATGGTGAC AACGAACTGG AGCTGGGCCA CGACGAGATC 
CTCGCCGACG GCCTCATCAA GAAGGGGATC AAGGAGCAGA CGCAACTGGA GGTGGCCATC 
GGGGATGGCA TGGAATTCAC GATACACGGC GACATGAAGA ACAACAAGCC GAAGAAATCC 
AAATATCTAA ATAACGCAAC GGACGACGAC ACTGCCAGCA TTAACTCATA TGGTAGCCAT 
AAGAATCGAC CATTCAAGGA CGAGAGCCAC AAGGGCAGCG CCGAGACGAT GGAGGGCGAG 
GAGAAGCGCG ACGCCAGCAA GGAGGATTTA GGTCTCGACG AGGAACTGGA CGAGGAGGGC 
GAATGCGAGG AGGGCCCGCT CGACGGTGAT ATCATTATTC ATGCACACGA CGAGGATATA 
CTCGATGAAT ATCCAGCTGA TTGCTGCCCC GATTCGTACT ATAAGAAATT TCCGATCTTA 
GCCGGTGACG ATGACTCGCC GTTCTGGCAA GGATGGGGCA ATTTACGACT GAAAACTTTT 
CAATTAATTG AAAATAAATA TTTTGAAACA GCTGTTATCA CTATGATTTT AATGAGTAGC 
TTAGCTTTGG CATTAGAAGA TGTACATCTG CCACAAAGAC CCATACTGCA GGATATTTTA 
TACTATATGG ACAGAATATT TACGGTTATA TTCTTCTTGG AAATGTTAAT CAAGTGGTTG 
GCGCTCGGCT TCAAAGTGTA CTTCACCAAC GCGTGGTGTT GGCTCGATTT CGTGATTGTC 
ATGGTATCGC TTATCAACTT CGTTGCTTCA CTTGTTGGAG CTGGTGGTAT TCAAGCCTTC 
AAGACTATGC GAACGTTAAG AGCACTGAGA CCACTACGTG CCATGTCCCG TATGCAGGGC 
ATGAGGGTCG TCGTTAATGC GCTGGTACAA GCTATACCGT CCATCTTCAA TGTGCTATTG 
GTGTGTCTAA TATTTTGGCT AATTTTTGCC ATAATGGGTG TACAGCTTTT TGCTGGAAAA 
TATTTTAAGT GCGAGGACAT GAATGGCACG AAGCTCAGCC ACGAGATCAT ACCAAATCGC 
AATGCCTGCG AGAGCGAGAA CTACACGTGG GTGAATTCAG CAATGAATTT CGATCATGTA 
GGTAACGCGT ATCTGTGCCT TTTCCAAGTG GCCACCTTCA AAGGCTGGAT ACAAATCATG 
AACGATGCTA TCGATTCACG AGAGGTGGAC AAGCAACCAA TTCGTGAAAC GAACATCTAC 
ATGTATTTAT ATTTCGTATT CTTCATCATA TTTGGATCAT TTTTCACACT CAATCTGTTC 
ATTGGTGTTA TCATTGATAA TTTTAATGAG CAAAAGAAAA AAGCAGGTGG ATCATTAGAA 
ATGTTCATGA CAGAAGATCA GAAAAAGTAC TATAATGCTA TGAAAAAGAT GGGCTCTAAA 
AAACCATTAA AAGCCATTCC AAGACCAAGG TGGCGACCAC AAGCAATAGT CTTTGAAATA 
GTAACCGATA AGAAATTCGA TATAATCATT ATGTTATTCA TTGGTCTGAA CATGTTCACC 
ATGACCCTCG ATCGTTACGA TGCGTCGGAC ACGTATAACG CGGTCCTAGA CTATCTCAAT 
GCGATATTCG TAGTTATTTT CAGTTCCGAA TGTCTATTAA AAATATTCGC TTTACGATAT 
CACTATTTTA TTGAGCCATG GAATTTATTT GATGTAGTAG TTGTCATTTT ATCCATCTTA 
GGTCTTGTAC TTAGCGATAT TATCGAGAAG TACTTCGTGT CGCCGACCCT GCTCCGAGTG 
GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT CGACTGGTGA AGGGAGCCAA GGGCATTCGG 
ACACTGCTCT TCGCGTTGGC CATGTCGCTG CCGGCCCTGT TCAACATCTG CCTGCTGCTG 
TTCCTGGTCA TGTTCATCTT TGCCATTTTC GGCATGTCGT TCTTCATGCA CGTGAAGGAG 
AAGAGCGGCA TCAACGACGT CTACAACTTC AAGACCTTTG GCCAGAGCAT GATCCTGCTC 
TTTCAGATGT CGACGTCAGC CGGTTGGGAT GGTGTACTGG ACGCCATTAT CAATGAGGAA 
GCATGCGATC CACCCGACAA CGACAAAGGC TATCCGGGCA ATTGTGGTTC AGCGACCGTT 
GGAATAACGT TTCTCCTCTC ATACCTAGTT ATAAGCTTTT TGATAGTTAT TAATATGTAC 
ATTGCTGTCA TTCTCGAGAA CTATAGTCAG GCCACCGAGG ACGTGCAAGA GGGTCTAACC 
GACGACGACT ACGACATGTA CTATGAGATC TGGCAGCAAT TCGATCCGGA GGGCACCCAG 
TACATACGCT ATGATCAGCT GTCCGAATTC CTGGACGTAC TGGAGCCCCC GCTGCAGATC 
CACAAACCGA ACAAGTACAA GATCATATCG ATGGACATAC CCATCTGTCG CGGTGACCTC 
ATGTACTGCG TCGACATCCT CGACGCCCTT ACGAAAGACT TCTTTGCGCG GAAGGGCAAT 
CCGATAGAGG AGACGGGTGA GATTGGTGAG ATAGCGGCCC GCCCGGATAC GGAGGGCTAC 
GAGCCCGTCT CATCAACGCT GTGGCGTCAG CGTGAGGAGT ACTGCGCCCG GCTAATCCAG 
CACGCCTGGC GAAAGCACAA GGCGCGCGGC GAGGGAGGTG GGTCCTTTGA GCCGGATACG 
GATCATGGCG ATGGCGGTGA TCCGGATGCC GGGGACCCGG CGCCCGATGA AGCAACGGAC 
GGCGATGCGC CCGCTGGTGG AGATGGTAGT GTTAACGGTA CTGCAGAAGG AGCTGCCGAT 
GCCGATGAGA GTAATGTAAA TAGTCCGGGT GAGGATGCAG CGGCGGCGGC AGCAGCAGCA 
GCAGCAGCGG CGGCGGCGGG CACGACGACG GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG 
CGACAGACCG CCGTTCTCGT GGAGAGCGAC GGGTTCGTGA CGAAGAACGG CCACAAGGTG 
GTCATCCACT CGCGATCGCC GAGCATCACG TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188641.1
CDS	269..6439
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTDYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201712.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AT, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201712.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GATTATGTGA CCATGGGTAT AGATTTAGGT 
AATCTAGCAG CCCTGCGAAC GTTTAGGGTG CTGCGAGCGC TTAAAACCGT AGCCATTGTG 
CCAGGCTTGA AGACCATCGT CGGCGCCGTC ATCGAATCGG TGAAGAATCT GCGCGATGTG 
ATTATCCTGA CCATGTTCTC CCTGTCGGTG TTCGCGTTGA TGGGCCTACA GATCTATATG 
GGCGTGCTCA CGCAGAAGTG CATCAAGAAG TTCCCGCTGG ACGGTTCCTG GGGCAATCTG 
ACCGACGAGA ACTGGGACTA TCACAATCGC AATAGCTCCA ATTGGTATTC CGAGGACGAG 
GGCATCTCAT TTCCGTTATG CGGCAATATA TCCGGTGCGG GGCAATGCGA CGACGATTAC 
GTGTGCCTGC AGGGGTTTGG TCCGAATCCG AATTATGGCT ACACCAGCTT CGATTCGTTC 
GGATGGGCTT TCCTGTCCGC CTTCCGGCTG ATGACACAGG ACTTCTGGGA GGATCTGTAC 
CAGCTGGTGT TGCGCGCCGC CGGACCATGG CACATGCTGT TCTTTATAGT CATCATCTTC 
CTAGGTTCAT TCTATCTTGT GAATTTGATT TTGGCCATTG TTGCCATGTC GTATGACGAA 
TTGCAAAAGA AGGCCGAAGA AGAAGAGGCT GCCGAAGAGG AGGCGATACG TGAAGCGGAA 
GAAGCTGCCG CCGCCAAAGC GGCCAAGCTG GAGGAGCGGG CCAATGCGCA GGCTCAGGCA 
GCAGCGGATG CGGCTGCCGC CGAAGAGGCT GCACTGCATC CGGAAATGGC CAAGAGTCCG 
ACGTATTCTT GCATCAGCTA TGAGCTATTT GTTGGCGGCG AGAAGGGCAA CGATGACAAC 
AACAAAGAGA AGATGTCCAT TCGGAGCGTC GAGGTGGAGT CGGAGTCGGT GAGCGTTATA 
CAAAGACAAC CAGCACCTAC CACAGCACAC CAAGCTACCA AAGTTCGTAA AGTGAGCACG 
TACACGATAC GGAACGGACG TGGCCGCTTT GGTATACCCG GTAGCGATCG TAAGCCATTG 
GTATTGTCAA CATATCAGGA TGCCCAGCAG CACTTGCCCT ATGCCGACGA CTCGAATGCC 
GTCACCCCGA TGTCCGAAGA GAATGGGGCC ATCATAGTGC CCGTGTACTA TGGCAATCTA 
GGCTCCCGAC ACTCATCGTA TACCTCGCAT CAGTCCCGAA TATCGTATAC CTCACATGGC 
GATCTACTCG GCGGCATGGC CGTCATGGGC GTCAGCACAA TGACCAAGGA GAGCAAATTG 
CGCAACCGGA ACACACGCAA TCAATCAGTG GGCGCCACCA ATGGCGGCAC CACCTGTCTG 
GACACCAATC ACAAGCTCGA TCATCGCGAC TACGAAATTG GCCTGGAGTG CACGGACGAA 
GCTGGCAAGA TTAAACATCA TGACAATCCT TTTATCGAGC CCGTCCAGAC ACAAACGGTG 
GTTGATATGA AAGATGTGAT GGTCCTGAAT GACATCATCG AACAGGCCGC TGGTCGGCAC 
AGTCGGGCAA GCGATCGCGG TGTCTCCGTT TACTATTTCC CAACAGAGGA CGATGACGAG 
GATGGGCCGA CGTTCAAAGA CAAGGCACTC GAAGTGATCC TCAAAGGCAT CGATGTGTTT 
TGTGTGTGGG ACTGTTGCTG GGTTTGGTTG AAATTTCAGG AGTGGGTATC GCTCATCGTC 
TTCGATCCCT TCGTCGAGCT CTTCATCACG CTGTGCATTG TGGTCAACAC GATGTTCATG 
GCAATGGATC ACCACGATAT GAACAAGGAG ATGGAACGCG TGCTCAAGAG TGGCAACTAT 
TTCTTCACCG CCACCTTTGC CATCGAGGCC ACCATGAAGC TAATGGCCAT GAGCCCCAAG 
TACTATTTCC AGGAGGGCTG GAACATCTTC GACTTCATTA TCGTGGCCCT ATCGCTATTG 
GAACTGGGAC TCGAGGGTGT CCAGGGTCTG TCCGTATTGC GTTCCTTTCG ATTGCTGCGT 
GTATTCAAAC TGGCCAAGTC TTGGCCCACA CTTAATTTAC TCATTTCGAT TATGGGACGC 
ACCATGGGCG CTTTGGGTAA TCTGACATTT GTACTTTGCA TTATCATCTT CATCTTTGCG 
GTGATGGGAA TGCAACTGTT CGGAAAGAAT TATCATGATC ACAAGGACCG CTTTCCGGAT 
GGCGACCTGC CGCGCTGGAA CTTCACCGAC TTTATGCACA GCTTCATGAT CGTGTTCCGG 
GTGCTCTGCG GAGAATGGAT CGAGTCCATG TGGGACTGCA TGTACGTGGG CGATGTCTCG 
TGCATTCCCT TCTTCTTGGC CACCGTTGTC ATCGGCAATC TTGTGGTACT TAACCTTTTC 
TTAGCCTTGC TTTTGTCCAA TTTTGGCTCA TCTAGCTTAT CAGCGCCGAC TGCCGATAAC 
GATACGAATA AAATAGCCGA GGCCTTCAAT CGAATTGGCC GATTTAAAAG TTGGGTTAAG 
CGTAATATTG CTGATTGTTT CAAGTTAATA CGTAACAAAT TGACAAATCA AATAAGTGAT 
CAACCATCAG GTGAGAGGAC CAACCAGATC AGTTGGATTT GGAGCGAAGG CAAAGGCGTT 
TGTCGTTGTA TATCTGCAGA GCATGGTGAC AACGAACTGG AGCTGGGCCA CGACGAGATC 
CTCGCCGACG GCCTCATCAA GAAGGGGATC AAGGAGCAGA CGCAACTGGA GGTGGCCATC 
GGGGATGGCA TGGAATTCAC GATACACGGC GACATGAAGA ACAACAAGCC GAAGAAATCC 
AAATATCTAA ATAACGCAAC GGACGACGAC ACTGCCAGCA TTAACTCATA TGGTAGCCAT 
AAGAATCGAC CATTCAAGGA CGAGAGCCAC AAGGGCAGCG CCGAGACGAT GGAGGGCGAG 
GAGAAGCGCG ACGCCAGCAA GGAGGATTTA GGTCTCGACG AGGAACTGGA CGAGGAGGGC 
GAATGCGAGG AGGGCCCGCT CGACGGTGAT ATCATTATTC ATGCACACGA CGAGGATATA 
CTCGATGAAT ATCCAGCTGA TTGCTGCCCC GATTCGTACT ATAAGAAATT TCCGATCTTA 
GCCGGTGACG ATGACTCGCC GTTCTGGCAA GGATGGGGCA ATTTACGACT GAAAACTTTT 
CAATTAATTG AAAATAAATA TTTTGAAACA GCTGTTATCA CTATGATTTT AATGAGTAGC 
TTAGCTTTGG CATTAGAAGA TGTACATCTG CCACAAAGAC CCATACTGCA GGATATTTTA 
TACTATATGG ACAGAATATT TACGGTTATA TTCTTCTTGG AAATGTTAAT CAAGTGGTTG 
GCGCTCGGCT TCAAAGTGTA CTTCACCAAC GCGTGGTGTT GGCTCGATTT CGTGATTGTC 
ATGGTATCGC TTATCAACTT CGTTGCTTCA CTTGTTGGAG CTGGTGGTAT TCAAGCCTTC 
AAGACTATGC GAACGTTAAG AGCACTGAGA CCACTACGTG CCATGTCCCG TATGCAGGGC 
ATGAGGGTCG TCGTTAATGC GCTGGTACAA GCTATACCGT CCATCTTCAA TGTGCTATTG 
GTGTGTCTAA TATTTTGGCT AATTTTTGCC ATAATGGGTG TACAGCTTTT TGCTGGAAAA 
TATTTTAAGT GCGAGGACAT GAATGGCACG AAGCTCAGCC ACGAGATCAT ACCAAATCGC 
AATGCCTGCG AGAGCGAGAA CTACACGTGG GTGAATTCAG CAATGAATTT CGATCATGTA 
GGTAACGCGT ATCTGTGCCT TTTCCAAGTG GCCACCTTCA AAGGCTGGAT ACAAATCATG 
AACGATGCTA TCGATTCACG AGAGGTGGAC AAGCAACCAA TTCGTGAAAC GAACATCTAC 
ATGTATTTAT ATTTCGTATT CTTCATCATA TTTGGATCAT TTTTCACACT CAATCTGTTC 
ATTGGTGTTA TCATTGATAA TTTTAATGAG CAAAAGAAAA AAGCAGGTGG ATCATTAGAA 
ATGTTCATGA CAGAAGATCA GAAAAAGTAC TATAATGCTA TGAAAAAGAT GGGCTCTAAA 
AAACCATTAA AAGCCATTCC AAGACCAAGG TGGCGACCAC AAGCAATAGT CTTTGAAATA 
GTAACCGATA AGAAATTCGA TATAATCATT ATGTTATTCA TTGGTCTGAA CATGTTCACC 
ATGACCCTCG ATCGTTACGA TGCGTCGGAC ACGTATAACG CGGTCCTAGA CTATCTCAAT 
GCGATATTCG TAGTTATTTT CAGTTCCGAA TGTCTATTAA AAATATTCGC TTTACGATAT 
CACTATTTTA TTGAGCCATG GAATTTATTT GATGTAGTAG TTGTCATTTT ATCCATCTTA 
GGTCTTGTAC TTAGCGATAT TATCGAGAAG TACTTCGTGT CGCCGACCCT GCTCCGAGTG 
GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT CGACTGGTGA AGGGAGCCAA GGGCATTCGG 
ACACTGCTCT TCGCGTTGGC CATGTCGCTG CCGGCCCTGT TCAACATCTG CCTGCTGCTG 
TTCCTGGTCA TGTTCATCTT TGCCATTTTC GGCATGTCGT TCTTCATGCA CGTGAAGGAG 
AAGAGCGGCA TCAACGACGT CTACAACTTC AAGACCTTTG GCCAGAGCAT GATCCTGCTC 
TTTCAGATGT CGACGTCAGC CGGTTGGGAT GGTGTACTGG ACGCCATTAT CAATGAGGAA 
GCATGCGATC CACCCGACAA CGACAAAGGC TATCCGGGCA ATTGTGGTTC AGCGACCGTT 
GGAATAACGT TTCTCCTCTC ATACCTAGTT ATAAGCTTTT TGATAGTTAT TAATATGTAC 
ATTGCTGTCA TTCTCGAGAA CTATAGTCAG GCCACCGAGG ACGTGCAAGA GGGTCTAACC 
GACGACGACT ACGACATGTA CTATGAGATC TGGCAGCAAT TCGATCCGGA GGGCACCCAG 
TACATACGCT ATGATCAGCT GTCCGAATTC CTGGACGTAC TGGAGCCCCC GCTGCAGATC 
CACAAACCGA ACAAGTACAA GATCATATCG ATGGACATAC CCATCTGTCG CGGTGACCTC 
ATGTACTGCG TCGACATCCT CGACGCCCTT ACGAAAGACT TCTTTGCGCG GAAGGGCAAT 
CCGATAGAGG AGACGGGTGA GATTGGTGAG ATAGCGGCCC GCCCGGATAC GGAGGGCTAC 
GAGCCCGTCT CATCAACGCT GTGGCGTCAG CGTGAGGAGT ACTGCGCCCG GCTAATCCAG 
CACGCCTGGC GAAAGCACAA GGCGCGCGGC GAGGGAGGTG GGTCCTTTGA GCCGGATACG 
GATCATGGCG ATGGCGGTGA TCCGGATGCC GGGGACCCGG CGCCCGATGA AGCAACGGAC 
GGCGATGCGC CCGCTGGTGG AGATGGTAGT GTTAACGGTA CTGCAGAAGG AGCTGCCGAT 
GCCGATGAGA GTAATGTAAA TAGTCCGGGT GAGGATGCAG CGGCGGCGGC AGCAGCAGCA 
GCAGCAGCGG CGGCGGCGGG CACGACGACG GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG 
CGACAGACCG CCGTTCTCGT GGAGAGCGAC GGGTTCGTGA CGAAGAACGG CCACAAGGTG 
GTCATCCACT CGCGATCGCC GAGCATCACG TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201711.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188640.1 Drosophila melanogaster paralytic (para), transcript variant AS, mRNA.

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CloneID	OFa03648
Clone ID Related Accession (Same CDS sequence)	NM_001201711.1
Accession Version	NM_001201711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AS
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GATTATGTGA CCATGGGTAT AGATTTAGGT 
AATCTAGCAG CCCTGCGAAC GTTTAGGGTG CTGCGAGCGC TTAAAACCGT AGCCATTGTG 
CCAGGCTTGA AGACCATCGT CGGCGCCGTC ATCGAATCGG TGAAGAATCT GCGCGATGTG 
ATTATCCTGA CCATGTTCTC CCTGTCGGTG TTCGCGTTGA TGGGCCTACA GATCTATATG 
GGCGTGCTCA CGCAGAAGTG CATCAAGAAG TTCCCGCTGG ACGGTTCCTG GGGCAATCTG 
ACCGACGAGA ACTGGGACTA TCACAATCGC AATAGCTCCA ATTGGTATTC CGAGGACGAG 
GGCATCTCAT TTCCGTTATG CGGCAATATA TCCGGTGCGG GGCAATGCGA CGACGATTAC 
GTGTGCCTGC AGGGGTTTGG TCCGAATCCG AATTATGGCT ACACCAGCTT CGATTCGTTC 
GGATGGGCTT TCCTGTCCGC CTTCCGGCTG ATGACACAGG ACTTCTGGGA GGATCTGTAC 
CAGCTGGTGT TGCGCGCCGC CGGACCATGG CACATGCTGT TCTTTATAGT CATCATCTTC 
CTAGGTTCAT TCTATCTTGT GAATTTGATT TTGGCCATTG TTGCCATGTC GTATGACGAA 
TTGCAAAAGA AGGCCGAAGA AGAAGAGGCT GCCGAAGAGG AGGCGATACG TGAAGCGGAA 
GAAGCTGCCG CCGCCAAAGC GGCCAAGCTG GAGGAGCGGG CCAATGCGCA GGCTCAGGCA 
GCAGCGGATG CGGCTGCCGC CGAAGAGGCT GCACTGCATC CGGAAATGGC CAAGAGTCCG 
ACGTATTCTT GCATCAGCTA TGAGCTATTT GTTGGCGGCG AGAAGGGCAA CGATGACAAC 
AACAAAGAGA AGATGTCCAT TCGGAGCGTC GAGGTGGAGT CGGAGTCGGT GAGCGTTATA 
CAAAGACAAC CAGCACCTAC CACAGCACAC CAAGCTACCA AAGTTCGTAA AGTGAGCACG 
ACATCCTTAT CCTTACCTGG TTCACCGTTT AACATACGCA GGGGATCACG TAGTTCTCAC 
AAGTACACGA TACGGAACGG ACGTGGCCGC TTTGGTATAC CCGGTAGCGA TCGTAAGCCA 
TTGGTATTGT CAACATATCA GGATGCCCAG CAGCACTTGC CCTATGCCGA CGACTCGAAT 
GCCGTCACCC CGATGTCCGA AGAGAATGGG GCCATCATAG TGCCCGTGTA CTATGGCAAT 
CTAGGCTCCC GACACTCATC GTATACCTCG CATCAGTCCC GAATATCGTA TACCTCACAT 
GGCGATCTAC TCGGCGGCAT GGCCGTCATG GGCGTCAGCA CAATGACCAA GGAGAGCAAA 
TTGCGCAACC GGAACACACG CAATCAATCA GTGGGCGCCA CCAATGGCGG CACCACCTGT 
CTGGACACCA ATCACAAGCT CGATCATCGC GACTACGAAA TTGGCCTGGA GTGCACGGAC 
GAAGCTGGCA AGATTAAACA TCATGACAAT CCTTTTATCG AGCCCGTCCA GACACAAACG 
GTGGTTGATA TGAAAGATGT GATGGTCCTG AATGACATCA TCGAACAGGC CGCTGGTCGG 
CACAGTCGGG CAAGCGATCG CGGTGTCTCC GTTTACTATT TCCCAACAGA GGACGATGAC 
GAGGATGGGC CGACGTTCAA AGACAAGGCA CTCGAAGTGA TCCTCAAAGG CATCGATGTG 
TTTTGTGTGT GGGACTGTTG CTGGGTTTGG TTGAAATTTC AGGAGTGGGT ATCGCTCATC 
GTCTTCGATC CCTTCGTCGA GCTCTTCATC ACGCTGTGCA TTGTGGTCAA CACGATGTTC 
ATGGCAATGG ATCACCACGA TATGAACAAG GAGATGGAAC GCGTGCTCAA GAGTGGCAAC 
TATTTCTTCA CCGCCACCTT TGCCATCGAG GCCACCATGA AGCTAATGGC CATGAGCCCC 
AAGTACTATT TCCAGGAGGG CTGGAACATC TTCGACTTCA TTATCGTGGC CCTATCGCTA 
TTGGAACTGG GACTCGAGGG TGTCCAGGGT CTGTCCGTAT TGCGTTCCTT TCGATTGCTG 
CGTGTATTCA AACTGGCCAA GTCTTGGCCC ACACTTAATT TACTCATTTC GATTATGGGA 
CGCACCATGG GCGCTTTGGG TAATCTGACA TTTGTACTTT GCATTATCAT CTTCATCTTT 
GCGGTGATGG GAATGCAACT GTTCGGAAAG AATTATCATG ATCACAAGGA CCGCTTTCCG 
GATGGCGACC TGCCGCGCTG GAACTTCACC GACTTTATGC ACAGCTTCAT GATCGTGTTC 
CGGGTGCTCT GCGGAGAATG GATCGAGTCC ATGTGGGACT GCATGTACGT GGGCGATGTC 
TCGTGCATTC CCTTCTTCTT GGCCACCGTT GTCATCGGCA ATCTTGTGGT ACTTAACCTT 
TTCTTAGCCT TGCTTTTGTC CAATTTTGGC TCATCTAGCT TATCAGCGCC GACTGCCGAT 
AACGATACGA ATAAAATAGC CGAGGCCTTC AATCGAATTG GCCGATTTAA AAGTTGGGTT 
AAGCGTAATA TTGCTGATTG TTTCAAGTTA ATACGTAACA AATTGACAAA TCAAATAAGT 
GATCAACCAT CAGGTGAGAG GACCAACCAG ATCAGTTGGA TTTGGAGCGA AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGGACGAC 
GACACTGCCA GCATTAACTC ATATGGTAGC CATAAGAATC GACCATTCAA GGACGAGAGC 
CACAAGGGCA GCGCCGAGAC GATGGAGGGC GAGGAGAAGC GCGACGCCAG CAAGGAGGAT 
TTAGGTCTCG ACGAGGAACT GGACGAGGAG GGCGAATGCG AGGAGGGCCC GCTCGACGGT 
GATATCATTA TTCATGCACA CGACGAGGAT ATACTCGATG AATATCCAGC TGATTGCTGC 
CCCGATTCGT ACTATAAGAA ATTTCCGATC TTAGCCGGTG ACGATGACTC GCCGTTCTGG 
CAAGGATGGG GCAATTTACG ACTGAAAACT TTTCAATTAA TTGAAAATAA ATATTTTGAA 
ACAGCTGTTA TCACTATGAT TTTAATGAGT AGCTTAGCTT TGGCATTAGA AGATGTACAT 
CTGCCACAAA GACCCATACT GCAGGATATT TTATACTATA TGGACAGAAT ATTTACGGTT 
ATATTCTTCT TGGAAATGTT AATCAAGTGG TTGGCGCTCG GCTTCAAAGT GTACTTCACC 
AACGCGTGGT GTTGGCTCGA TTTCGTGATT GTCATGGTAT CGCTTATCAA CTTCGTTGCT 
TCACTTGTTG GAGCTGGTGG TATTCAAGCC TTCAAGACTA TGCGAACGTT AAGAGCACTG 
AGACCACTAC GTGCCATGTC CCGTATGCAG GGCATGAGGG TCGTCGTTAA TGCGCTGGTA 
CAAGCTATAC CGTCCATCTT CAATGTGCTA TTGGTGTGTC TAATATTTTG GCTAATTTTT 
GCCATAATGG GTGTACAGCT TTTTGCTGGA AAATATTTTA AGTGCGAGGA CATGAATGGC 
ACGAAGCTCA GCCACGAGAT CATACCAAAT CGCAATGCCT GCGAGAGCGA GAACTACACG 
TGGGTGAATT CAGCAATGAA TTTCGATCAT GTAGGTAACG CGTATCTGTG CCTTTTCCAA 
GTGGCCACCT TCAAAGGCTG GATACAAATC ATGAACGATG CTATCGATTC ACGAGAGGTG 
GACAAGCAAC CAATTCGTGA AACGAACATC TACATGTATT TATATTTCGT ATTCTTCATC 
ATATTTGGAT CATTTTTCAC ACTCAATCTG TTCATTGGTG TTATCATTGA TAATTTTAAT 
GAGCAAAAGA AAAAAGCAGG TGGATCATTA GAAATGTTCA TGACAGAAGA TCAGAAAAAG 
TACTATAATG CTATGAAAAA GATGGGCTCT AAAAAACCAT TAAAAGCCAT TCCAAGACCA 
AGGTGGCGAC CACAAGCAAT AGTCTTTGAA ATAGTAACCG ATAAGAAATT CGATATAATC 
ATTATGTTAT TCATTGGTCT GAACATGTTC ACCATGACCC TCGATCGTTA CGATGCGTCG 
GACACGTATA ACGCGGTCCT AGACTATCTC AATGCGATAT TCGTAGTTAT TTTCAGTTCC 
GAATGTCTAT TAAAAATATT CGCTTTACGA TATCACTATT TTATTGAGCC ATGGAATTTA 
TTTGATGTAG TAGTTGTCAT TTTATCCATC TTAGGTCTTG TACTTAGCGA TATTATCGAG 
AAGTACTTCG TGTCGCCGAC CCTGCTCCGA GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC 
CTTCGACTGG TGAAGGGAGC CAAGGGCATT CGGACACTGC TCTTCGCGTT GGCCATGTCG 
CTGCCGGCCC TGTTCAACAT CTGCCTGCTG CTGTTCCTGG TCATGTTCAT CTTTGCCATT 
TTCGGCATGT CGTTCTTCAT GCACGTGAAG GAGAAGAGCG GCATCAACGA CGTCTACAAC 
TTCAAGACCT TTGGCCAGAG CATGATCCTG CTCTTTCAGA TGTCGACGTC AGCCGGTTGG 
GATGGTGTAC TGGACGCCAT TATCAATGAG GAAGCATGCG ATCCACCCGA CAACGACAAA 
GGCTATCCGG GCAATTGTGG TTCAGCGACC GTTGGAATAA CGTTTCTCCT CTCATACCTA 
GTTATAAGCT TTTTGATAGT TATTAATATG TACATTGCTG TCATTCTCGA GAACTATAGT 
CAGGCCACCG AGGACGTGCA AGAGGGTCTA ACCGACGACG ACTACGACAT GTACTATGAG 
ATCTGGCAGC AATTCGATCC GGAGGGCACC CAGTACATAC GCTATGATCA GCTGTCCGAA 
TTCCTGGACG TACTGGAGCC CCCGCTGCAG ATCCACAAAC CGAACAAGTA CAAGATCATA 
TCGATGGACA TACCCATCTG TCGCGGTGAC CTCATGTACT GCGTCGACAT CCTCGACGCC 
CTTACGAAAG ACTTCTTTGC GCGGAAGGGC AATCCGATAG AGGAGACGGG TGAGATTGGT 
GAGATAGCGG CCCGCCCGGA TACGGAGGGC TACGAGCCCG TCTCATCAAC GCTGTGGCGT 
CAGCGTGAGG AGTACTGCGC CCGGCTAATC CAGCACGCCT GGCGAAAGCA CAAGGCGCGC 
GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT ACGGATCATG GCGATGGCGG TGATCCGGAT 
GCCGGGGACC CGGCGCCCGA TGAAGCAACG GACGGCGATG CGCCCGCTGG TGGAGATGGT 
AGTGTTAACG GTACTGCAGA AGGAGCTGCC GATGCCGATG AGAGTAATGT AAATAGTCCG 
GGTGAGGATG CAGCGGCGGC GGCAGCAGCA GCAGCAGCAG CGGCGGCGGC GGGCACGACG 
ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC GGGCGACAGA CCGCCGTTCT CGTGGAGAGC 
GACGGGTTCG TGACGAAGAA CGGCCACAAG GTGGTCATCC ACTCGCGATC GCCGAGCATC 
ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188640.1
CDS	269..6472
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTDYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201711.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AS, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201711.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GATTATGTGA CCATGGGTAT AGATTTAGGT 
AATCTAGCAG CCCTGCGAAC GTTTAGGGTG CTGCGAGCGC TTAAAACCGT AGCCATTGTG 
CCAGGCTTGA AGACCATCGT CGGCGCCGTC ATCGAATCGG TGAAGAATCT GCGCGATGTG 
ATTATCCTGA CCATGTTCTC CCTGTCGGTG TTCGCGTTGA TGGGCCTACA GATCTATATG 
GGCGTGCTCA CGCAGAAGTG CATCAAGAAG TTCCCGCTGG ACGGTTCCTG GGGCAATCTG 
ACCGACGAGA ACTGGGACTA TCACAATCGC AATAGCTCCA ATTGGTATTC CGAGGACGAG 
GGCATCTCAT TTCCGTTATG CGGCAATATA TCCGGTGCGG GGCAATGCGA CGACGATTAC 
GTGTGCCTGC AGGGGTTTGG TCCGAATCCG AATTATGGCT ACACCAGCTT CGATTCGTTC 
GGATGGGCTT TCCTGTCCGC CTTCCGGCTG ATGACACAGG ACTTCTGGGA GGATCTGTAC 
CAGCTGGTGT TGCGCGCCGC CGGACCATGG CACATGCTGT TCTTTATAGT CATCATCTTC 
CTAGGTTCAT TCTATCTTGT GAATTTGATT TTGGCCATTG TTGCCATGTC GTATGACGAA 
TTGCAAAAGA AGGCCGAAGA AGAAGAGGCT GCCGAAGAGG AGGCGATACG TGAAGCGGAA 
GAAGCTGCCG CCGCCAAAGC GGCCAAGCTG GAGGAGCGGG CCAATGCGCA GGCTCAGGCA 
GCAGCGGATG CGGCTGCCGC CGAAGAGGCT GCACTGCATC CGGAAATGGC CAAGAGTCCG 
ACGTATTCTT GCATCAGCTA TGAGCTATTT GTTGGCGGCG AGAAGGGCAA CGATGACAAC 
AACAAAGAGA AGATGTCCAT TCGGAGCGTC GAGGTGGAGT CGGAGTCGGT GAGCGTTATA 
CAAAGACAAC CAGCACCTAC CACAGCACAC CAAGCTACCA AAGTTCGTAA AGTGAGCACG 
ACATCCTTAT CCTTACCTGG TTCACCGTTT AACATACGCA GGGGATCACG TAGTTCTCAC 
AAGTACACGA TACGGAACGG ACGTGGCCGC TTTGGTATAC CCGGTAGCGA TCGTAAGCCA 
TTGGTATTGT CAACATATCA GGATGCCCAG CAGCACTTGC CCTATGCCGA CGACTCGAAT 
GCCGTCACCC CGATGTCCGA AGAGAATGGG GCCATCATAG TGCCCGTGTA CTATGGCAAT 
CTAGGCTCCC GACACTCATC GTATACCTCG CATCAGTCCC GAATATCGTA TACCTCACAT 
GGCGATCTAC TCGGCGGCAT GGCCGTCATG GGCGTCAGCA CAATGACCAA GGAGAGCAAA 
TTGCGCAACC GGAACACACG CAATCAATCA GTGGGCGCCA CCAATGGCGG CACCACCTGT 
CTGGACACCA ATCACAAGCT CGATCATCGC GACTACGAAA TTGGCCTGGA GTGCACGGAC 
GAAGCTGGCA AGATTAAACA TCATGACAAT CCTTTTATCG AGCCCGTCCA GACACAAACG 
GTGGTTGATA TGAAAGATGT GATGGTCCTG AATGACATCA TCGAACAGGC CGCTGGTCGG 
CACAGTCGGG CAAGCGATCG CGGTGTCTCC GTTTACTATT TCCCAACAGA GGACGATGAC 
GAGGATGGGC CGACGTTCAA AGACAAGGCA CTCGAAGTGA TCCTCAAAGG CATCGATGTG 
TTTTGTGTGT GGGACTGTTG CTGGGTTTGG TTGAAATTTC AGGAGTGGGT ATCGCTCATC 
GTCTTCGATC CCTTCGTCGA GCTCTTCATC ACGCTGTGCA TTGTGGTCAA CACGATGTTC 
ATGGCAATGG ATCACCACGA TATGAACAAG GAGATGGAAC GCGTGCTCAA GAGTGGCAAC 
TATTTCTTCA CCGCCACCTT TGCCATCGAG GCCACCATGA AGCTAATGGC CATGAGCCCC 
AAGTACTATT TCCAGGAGGG CTGGAACATC TTCGACTTCA TTATCGTGGC CCTATCGCTA 
TTGGAACTGG GACTCGAGGG TGTCCAGGGT CTGTCCGTAT TGCGTTCCTT TCGATTGCTG 
CGTGTATTCA AACTGGCCAA GTCTTGGCCC ACACTTAATT TACTCATTTC GATTATGGGA 
CGCACCATGG GCGCTTTGGG TAATCTGACA TTTGTACTTT GCATTATCAT CTTCATCTTT 
GCGGTGATGG GAATGCAACT GTTCGGAAAG AATTATCATG ATCACAAGGA CCGCTTTCCG 
GATGGCGACC TGCCGCGCTG GAACTTCACC GACTTTATGC ACAGCTTCAT GATCGTGTTC 
CGGGTGCTCT GCGGAGAATG GATCGAGTCC ATGTGGGACT GCATGTACGT GGGCGATGTC 
TCGTGCATTC CCTTCTTCTT GGCCACCGTT GTCATCGGCA ATCTTGTGGT ACTTAACCTT 
TTCTTAGCCT TGCTTTTGTC CAATTTTGGC TCATCTAGCT TATCAGCGCC GACTGCCGAT 
AACGATACGA ATAAAATAGC CGAGGCCTTC AATCGAATTG GCCGATTTAA AAGTTGGGTT 
AAGCGTAATA TTGCTGATTG TTTCAAGTTA ATACGTAACA AATTGACAAA TCAAATAAGT 
GATCAACCAT CAGGTGAGAG GACCAACCAG ATCAGTTGGA TTTGGAGCGA AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGGACGAC 
GACACTGCCA GCATTAACTC ATATGGTAGC CATAAGAATC GACCATTCAA GGACGAGAGC 
CACAAGGGCA GCGCCGAGAC GATGGAGGGC GAGGAGAAGC GCGACGCCAG CAAGGAGGAT 
TTAGGTCTCG ACGAGGAACT GGACGAGGAG GGCGAATGCG AGGAGGGCCC GCTCGACGGT 
GATATCATTA TTCATGCACA CGACGAGGAT ATACTCGATG AATATCCAGC TGATTGCTGC 
CCCGATTCGT ACTATAAGAA ATTTCCGATC TTAGCCGGTG ACGATGACTC GCCGTTCTGG 
CAAGGATGGG GCAATTTACG ACTGAAAACT TTTCAATTAA TTGAAAATAA ATATTTTGAA 
ACAGCTGTTA TCACTATGAT TTTAATGAGT AGCTTAGCTT TGGCATTAGA AGATGTACAT 
CTGCCACAAA GACCCATACT GCAGGATATT TTATACTATA TGGACAGAAT ATTTACGGTT 
ATATTCTTCT TGGAAATGTT AATCAAGTGG TTGGCGCTCG GCTTCAAAGT GTACTTCACC 
AACGCGTGGT GTTGGCTCGA TTTCGTGATT GTCATGGTAT CGCTTATCAA CTTCGTTGCT 
TCACTTGTTG GAGCTGGTGG TATTCAAGCC TTCAAGACTA TGCGAACGTT AAGAGCACTG 
AGACCACTAC GTGCCATGTC CCGTATGCAG GGCATGAGGG TCGTCGTTAA TGCGCTGGTA 
CAAGCTATAC CGTCCATCTT CAATGTGCTA TTGGTGTGTC TAATATTTTG GCTAATTTTT 
GCCATAATGG GTGTACAGCT TTTTGCTGGA AAATATTTTA AGTGCGAGGA CATGAATGGC 
ACGAAGCTCA GCCACGAGAT CATACCAAAT CGCAATGCCT GCGAGAGCGA GAACTACACG 
TGGGTGAATT CAGCAATGAA TTTCGATCAT GTAGGTAACG CGTATCTGTG CCTTTTCCAA 
GTGGCCACCT TCAAAGGCTG GATACAAATC ATGAACGATG CTATCGATTC ACGAGAGGTG 
GACAAGCAAC CAATTCGTGA AACGAACATC TACATGTATT TATATTTCGT ATTCTTCATC 
ATATTTGGAT CATTTTTCAC ACTCAATCTG TTCATTGGTG TTATCATTGA TAATTTTAAT 
GAGCAAAAGA AAAAAGCAGG TGGATCATTA GAAATGTTCA TGACAGAAGA TCAGAAAAAG 
TACTATAATG CTATGAAAAA GATGGGCTCT AAAAAACCAT TAAAAGCCAT TCCAAGACCA 
AGGTGGCGAC CACAAGCAAT AGTCTTTGAA ATAGTAACCG ATAAGAAATT CGATATAATC 
ATTATGTTAT TCATTGGTCT GAACATGTTC ACCATGACCC TCGATCGTTA CGATGCGTCG 
GACACGTATA ACGCGGTCCT AGACTATCTC AATGCGATAT TCGTAGTTAT TTTCAGTTCC 
GAATGTCTAT TAAAAATATT CGCTTTACGA TATCACTATT TTATTGAGCC ATGGAATTTA 
TTTGATGTAG TAGTTGTCAT TTTATCCATC TTAGGTCTTG TACTTAGCGA TATTATCGAG 
AAGTACTTCG TGTCGCCGAC CCTGCTCCGA GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC 
CTTCGACTGG TGAAGGGAGC CAAGGGCATT CGGACACTGC TCTTCGCGTT GGCCATGTCG 
CTGCCGGCCC TGTTCAACAT CTGCCTGCTG CTGTTCCTGG TCATGTTCAT CTTTGCCATT 
TTCGGCATGT CGTTCTTCAT GCACGTGAAG GAGAAGAGCG GCATCAACGA CGTCTACAAC 
TTCAAGACCT TTGGCCAGAG CATGATCCTG CTCTTTCAGA TGTCGACGTC AGCCGGTTGG 
GATGGTGTAC TGGACGCCAT TATCAATGAG GAAGCATGCG ATCCACCCGA CAACGACAAA 
GGCTATCCGG GCAATTGTGG TTCAGCGACC GTTGGAATAA CGTTTCTCCT CTCATACCTA 
GTTATAAGCT TTTTGATAGT TATTAATATG TACATTGCTG TCATTCTCGA GAACTATAGT 
CAGGCCACCG AGGACGTGCA AGAGGGTCTA ACCGACGACG ACTACGACAT GTACTATGAG 
ATCTGGCAGC AATTCGATCC GGAGGGCACC CAGTACATAC GCTATGATCA GCTGTCCGAA 
TTCCTGGACG TACTGGAGCC CCCGCTGCAG ATCCACAAAC CGAACAAGTA CAAGATCATA 
TCGATGGACA TACCCATCTG TCGCGGTGAC CTCATGTACT GCGTCGACAT CCTCGACGCC 
CTTACGAAAG ACTTCTTTGC GCGGAAGGGC AATCCGATAG AGGAGACGGG TGAGATTGGT 
GAGATAGCGG CCCGCCCGGA TACGGAGGGC TACGAGCCCG TCTCATCAAC GCTGTGGCGT 
CAGCGTGAGG AGTACTGCGC CCGGCTAATC CAGCACGCCT GGCGAAAGCA CAAGGCGCGC 
GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT ACGGATCATG GCGATGGCGG TGATCCGGAT 
GCCGGGGACC CGGCGCCCGA TGAAGCAACG GACGGCGATG CGCCCGCTGG TGGAGATGGT 
AGTGTTAACG GTACTGCAGA AGGAGCTGCC GATGCCGATG AGAGTAATGT AAATAGTCCG 
GGTGAGGATG CAGCGGCGGC GGCAGCAGCA GCAGCAGCAG CGGCGGCGGC GGGCACGACG 
ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC GGGCGACAGA CCGCCGTTCT CGTGGAGAGC 
GACGGGTTCG TGACGAAGAA CGGCCACAAG GTGGTCATCC ACTCGCGATC GCCGAGCATC 
ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201715.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188644.1 Drosophila melanogaster paralytic (para), transcript variant AW, mRNA.

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CloneID	OFa03650
Clone ID Related Accession (Same CDS sequence)	NM_001201715.1
Accession Version	NM_001201715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AW
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGTAC 
ACGATACGGA ACGGACGTGG CCGCTTTGGT ATACCCGGTA GCGATCGTAA GCCATTGGTA 
TTGTCAACAT ATCAGGATGC CCAGCAGCAC TTGCCCTATG CCGACGACTC GAATGCCGTC 
ACCCCGATGT CCGAAGAGAA TGGGGCCATC ATAGTGCCCG TGTACTATGG CAATCTAGGC 
TCCCGACACT CATCGTATAC CTCGCATCAG TCCCGAATAT CGTATACCTC ACATGGCGAT 
CTACTCGGCG GCATGGCCGT CATGGGCGTC AGCACAATGA CCAAGGAGAG CAAATTGCGC 
AACCGGAACA CACGCAATCA ATCAGTGGGC GCCACCAATG GCGGCACCAC CTGTCTGGAC 
ACCAATCACA AGCTCGATCA TCGCGACTAC GAAATTGGCC TGGAGTGCAC GGACGAAGCT 
GGCAAGATTA AACATCATGA CAATCCTTTT ATCGAGCCCG TCCAGACACA AACGGTGGTT 
GATATGAAAG ATGTGATGGT CCTGAATGAC ATCATCGAAC AGGCCGCTGG TCGGCACAGT 
CGGGCAAGCG ATCGCGGTGT CTCCGTTTAC TATTTCCCAA CAGAGGACGA TGACGAGGAT 
GGGCCGACGT TCAAAGACAA GGCACTCGAA GTGATCCTCA AAGGCATCGA TGTGTTTTGT 
GTGTGGGACT GTTGCTGGGT TTGGTTGAAA TTTCAGGAGT GGGTATCGCT CATCGTCTTC 
GATCCCTTCG TCGAGCTCTT CATCACGCTG TGCATTGTGG TCAACACGAT GTTCATGGCA 
ATGGATCACC ACGATATGAA CAAGGAGATG GAACGCGTGC TCAAGAGTGG CAACTATTTC 
TTCACCGCCA CCTTTGCCAT CGAGGCCACC ATGAAGCTAA TGGCCATGAG CCCCAAGTAC 
TATTTCCAGG AGGGCTGGAA CATCTTCGAC TTCATTATCG TGGCCCTATC GCTATTGGAA 
CTGGGACTCG AGGGTGTCCA GGGTCTGTCC GTATTGCGTT CCTTTCGATT GCTGCGTGTA 
TTCAAACTGG CCAAGTCTTG GCCCACACTT AATTTACTCA TTTCGATTAT GGGACGCACC 
ATGGGCGCTT TGGGTAATCT GACATTTGTA CTTTGCATTA TCATCTTCAT CTTTGCGGTG 
ATGGGAATGC AACTGTTCGG AAAGAATTAT CATGATCACA AGGACCGCTT TCCGGATGGC 
GACCTGCCGC GCTGGAACTT CACCGACTTT ATGCACAGCT TCATGATCGT GTTCCGGGTG 
CTCTGCGGAG AATGGATCGA GTCCATGTGG GACTGCATGT ACGTGGGCGA TGTCTCGTGC 
ATTCCCTTCT TCTTGGCCAC CGTTGTCATC GGCAATCTTG TGGTACTTAA CCTTTTCTTA 
GCCTTGCTTT TGTCCAATTT TGGCTCATCT AGCTTATCAG CGCCGACTGC CGATAACGAT 
ACGAATAAAA TAGCCGAGGC CTTCAATCGA ATTGGCCGAT TTAAAAGTTG GGTTAAGCGT 
AATATTGCTG ATTGTTTCAA GTTAATACGT AACAAATTGA CAAATCAAAT AAGTGATCAA 
CCATCAGGCA AAGGCGTTTG TCGTTGTATA TCTGCAGAGC ATGGTGACAA CGAACTGGAG 
CTGGGCCACG ACGAGATCCT CGCCGACGGC CTCATCAAGA AGGGGATCAA GGAGCAGACG 
CAACTGGAGG TGGCCATCGG GGATGGCATG GAATTCACGA TACACGGCGA CATGAAGAAC 
AACAAGCCGA AGAAATCCAA ATATCTAAAT AACGCAACGG ACGACGACAC TGCCAGCATT 
AACTCATATG GTAGCCATAA GAATCGACCA TTCAAGGACG AGAGCCACAA GGGCAGCGCC 
GAGACGATGG AGGGCGAGGA GAAGCGCGAC GCCAGCAAGG AGGATTTAGG TCTCGACGAG 
GAACTGGACG AGGAGGGCGA ATGCGAGGAG GGCCCGCTCG ACGGTGATAT CATTATTCAT 
GCACACGACG AGGATATACT CGATGAATAT CCAGCTGATT GCTGCCCCGA TTCGTACTAT 
AAGAAATTTC CGATCTTAGC CGGTGACGAT GACTCGCCGT TCTGGCAAGG ATGGGGCAAT 
TTACGACTGA AAACTTTTCA ATTAATTGAA AATAAATATT TTGAAACAGC TGTTATCACT 
ATGATTTTAA TGAGTAGCTT AGCTTTGGCA TTAGAAGATG TACATCTGCC ACAAAGACCC 
ATACTGCAGG ATATTTTATA CTATATGGAC AGAATATTTA CGGTTATATT CTTCTTGGAA 
ATGTTAATCA AGTGGTTGGC GCTCGGCTTC AAAGTGTACT TCACCAACGC GTGGTGTTGG 
CTCGATTTCG TGATTGTCAT GGTATCGCTT ATCAACTTCG TTGCTTCACT TGTTGGAGCT 
GGTGGTATTC AAGCCTTCAA GACTATGCGA ACGTTAAGAG CACTGAGACC ACTACGTGCC 
ATGTCCCGTA TGCAGGGCAT GAGGGTCGTC GTTAATGCGC TGGTACAAGC TATACCGTCC 
ATCTTCAATG TGCTATTGGT GTGTCTAATA TTTTGGCTAA TTTTTGCCAT AATGGGTGTA 
CAGCTTTTTG CTGGAAAATA TTTTAAGTGC GAGGACATGA ATGGCACGAA GCTCAGCCAC 
GAGATCATAC CAAATCGCAA TGCCTGCGAG AGCGAGAACT ACACGTGGGT GAATTCAGCA 
ATGAATTTCG ATCATGTAGG TAACGCGTAT CTGTGCCTTT TCCAAGTGGC CACCTTCAAA 
GGCTGGATAC AAATCATGAA CGATGCTATC GATTCACGAG AGGTGGACAA GCAACCAATT 
CGTGAAACGA ACATCTACAT GTATTTATAT TTCGTATTCT TCATCATATT TGGATCATTT 
TTCACACTCA ATCTGTTCAT TGGTGTTATC ATTGATAATT TTAATGAGCA AAAGAAAAAA 
GCAGGTGGAT CATTAGAAAT GTTCATGACA GAAGATCAGA AAAAGTACTA TAATGCTATG 
AAAAAGATGG GCTCTAAAAA ACCATTAAAA GCCATTCCAA GACCAAGGTG GCGACCACAA 
GCAATAGTCT TTGAAATAGT AACCGATAAG AAATTCGATA TAATCATTAT GTTATTCATT 
GGTCTGAACA TGTTCACCAT GACCCTCGAT CGTTACGATG CGTCGGACAC GTATAACGCG 
GTCCTAGACT ATCTCAATGC GATATTCGTA GTTATTTTCA GTTCCGAATG TCTATTAAAA 
ATATTCGCTT TACGATATCA CTATTTTATT GAGCCATGGA ATTTATTTGA TGTAGTAGTT 
GTCATTTTAT CCATCTTAGG TCTTGTACTT AGCGATATTA TCGAGAAGTA CTTCGTGTCG 
CCGACCCTGC TCCGAGTGGT GCGTGTGGCG AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG 
GGAGCCAAGG GCATTCGGAC ACTGCTCTTC GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC 
AACATCTGCC TGCTGCTGTT CCTGGTCATG TTCATCTTTG CCATTTTCGG CATGTCGTTC 
TTCATGCACG TGAAGGAGAA GAGCGGCATC AACGACGTCT ACAACTTCAA GACCTTTGGC 
CAGAGCATGA TCCTGCTCTT TCAGATGTCG ACGTCAGCCG GTTGGGATGG TGTACTGGAC 
GCCATTATCA ATGAGGAAGC ATGCGATCCA CCCGACAACG ACAAAGGCTA TCCGGGCAAT 
TGTGGTTCAG CGACCGTTGG AATAACGTTT CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG 
ATAGTTATTA ATATGTACAT TGCTGTCATT CTCGAGAACT ATAGTCAGGC CACCGAGGAC 
GTGCAAGAGG GTCTAACCGA CGACGACTAC GACATGTACT ATGAGATCTG GCAGCAATTC 
GATCCGGAGG GCACCCAGTA CATACGCTAT GATCAGCTGT CCGAATTCCT GGACGTACTG 
GAGCCCCCGC TGCAGATCCA CAAACCGAAC AAGTACAAGA TCATATCGAT GGACATACCC 
ATCTGTCGCG GTGACCTCAT GTACTGCGTC GACATCCTCG ACGCCCTTAC GAAAGACTTC 
TTTGCGCGGA AGGGCAATCC GATAGAGGAG ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC 
CCGGATACGG AGGGCTACGA GCCCGTCTCA TCAACGCTGT GGCGTCAGCG TGAGGAGTAC 
TGCGCCCGGC TAATCCAGCA CGCCTGGCGA AAGCACAAGG CGCGCGGCGA GGGAGGTGGG 
TCCTTTGAGC CGGATACGGA TCATGGCGAT GGCGGTGATC CGGATGCCGG GGACCCGGCG 
CCCGATGAAG CAACGGACGG CGATGCGCCC GCTGGTGGAG ATGGTAGTGT TAACGGTACT 
GCAGAAGGAG CTGCCGATGC CGATGAGAGT AATGTAAATA GTCCGGGTGA GGATGCAGCG 
GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG GCGGCGGGCA CGACGACGGC GGGAAGTCCC 
GGAGCGGGTA GCGCCGGGCG ACAGACCGCC GTTCTCGTGG AGAGCGACGG GTTCGTGACG 
AAGAACGGCC ACAAGGTGGT CATCCACTCG CGATCGCCGA GCATCACGTC GCGCACGGCG 
GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188644.1
CDS	269..6457
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201715.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AW, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201715.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGTAC 
ACGATACGGA ACGGACGTGG CCGCTTTGGT ATACCCGGTA GCGATCGTAA GCCATTGGTA 
TTGTCAACAT ATCAGGATGC CCAGCAGCAC TTGCCCTATG CCGACGACTC GAATGCCGTC 
ACCCCGATGT CCGAAGAGAA TGGGGCCATC ATAGTGCCCG TGTACTATGG CAATCTAGGC 
TCCCGACACT CATCGTATAC CTCGCATCAG TCCCGAATAT CGTATACCTC ACATGGCGAT 
CTACTCGGCG GCATGGCCGT CATGGGCGTC AGCACAATGA CCAAGGAGAG CAAATTGCGC 
AACCGGAACA CACGCAATCA ATCAGTGGGC GCCACCAATG GCGGCACCAC CTGTCTGGAC 
ACCAATCACA AGCTCGATCA TCGCGACTAC GAAATTGGCC TGGAGTGCAC GGACGAAGCT 
GGCAAGATTA AACATCATGA CAATCCTTTT ATCGAGCCCG TCCAGACACA AACGGTGGTT 
GATATGAAAG ATGTGATGGT CCTGAATGAC ATCATCGAAC AGGCCGCTGG TCGGCACAGT 
CGGGCAAGCG ATCGCGGTGT CTCCGTTTAC TATTTCCCAA CAGAGGACGA TGACGAGGAT 
GGGCCGACGT TCAAAGACAA GGCACTCGAA GTGATCCTCA AAGGCATCGA TGTGTTTTGT 
GTGTGGGACT GTTGCTGGGT TTGGTTGAAA TTTCAGGAGT GGGTATCGCT CATCGTCTTC 
GATCCCTTCG TCGAGCTCTT CATCACGCTG TGCATTGTGG TCAACACGAT GTTCATGGCA 
ATGGATCACC ACGATATGAA CAAGGAGATG GAACGCGTGC TCAAGAGTGG CAACTATTTC 
TTCACCGCCA CCTTTGCCAT CGAGGCCACC ATGAAGCTAA TGGCCATGAG CCCCAAGTAC 
TATTTCCAGG AGGGCTGGAA CATCTTCGAC TTCATTATCG TGGCCCTATC GCTATTGGAA 
CTGGGACTCG AGGGTGTCCA GGGTCTGTCC GTATTGCGTT CCTTTCGATT GCTGCGTGTA 
TTCAAACTGG CCAAGTCTTG GCCCACACTT AATTTACTCA TTTCGATTAT GGGACGCACC 
ATGGGCGCTT TGGGTAATCT GACATTTGTA CTTTGCATTA TCATCTTCAT CTTTGCGGTG 
ATGGGAATGC AACTGTTCGG AAAGAATTAT CATGATCACA AGGACCGCTT TCCGGATGGC 
GACCTGCCGC GCTGGAACTT CACCGACTTT ATGCACAGCT TCATGATCGT GTTCCGGGTG 
CTCTGCGGAG AATGGATCGA GTCCATGTGG GACTGCATGT ACGTGGGCGA TGTCTCGTGC 
ATTCCCTTCT TCTTGGCCAC CGTTGTCATC GGCAATCTTG TGGTACTTAA CCTTTTCTTA 
GCCTTGCTTT TGTCCAATTT TGGCTCATCT AGCTTATCAG CGCCGACTGC CGATAACGAT 
ACGAATAAAA TAGCCGAGGC CTTCAATCGA ATTGGCCGAT TTAAAAGTTG GGTTAAGCGT 
AATATTGCTG ATTGTTTCAA GTTAATACGT AACAAATTGA CAAATCAAAT AAGTGATCAA 
CCATCAGGCA AAGGCGTTTG TCGTTGTATA TCTGCAGAGC ATGGTGACAA CGAACTGGAG 
CTGGGCCACG ACGAGATCCT CGCCGACGGC CTCATCAAGA AGGGGATCAA GGAGCAGACG 
CAACTGGAGG TGGCCATCGG GGATGGCATG GAATTCACGA TACACGGCGA CATGAAGAAC 
AACAAGCCGA AGAAATCCAA ATATCTAAAT AACGCAACGG ACGACGACAC TGCCAGCATT 
AACTCATATG GTAGCCATAA GAATCGACCA TTCAAGGACG AGAGCCACAA GGGCAGCGCC 
GAGACGATGG AGGGCGAGGA GAAGCGCGAC GCCAGCAAGG AGGATTTAGG TCTCGACGAG 
GAACTGGACG AGGAGGGCGA ATGCGAGGAG GGCCCGCTCG ACGGTGATAT CATTATTCAT 
GCACACGACG AGGATATACT CGATGAATAT CCAGCTGATT GCTGCCCCGA TTCGTACTAT 
AAGAAATTTC CGATCTTAGC CGGTGACGAT GACTCGCCGT TCTGGCAAGG ATGGGGCAAT 
TTACGACTGA AAACTTTTCA ATTAATTGAA AATAAATATT TTGAAACAGC TGTTATCACT 
ATGATTTTAA TGAGTAGCTT AGCTTTGGCA TTAGAAGATG TACATCTGCC ACAAAGACCC 
ATACTGCAGG ATATTTTATA CTATATGGAC AGAATATTTA CGGTTATATT CTTCTTGGAA 
ATGTTAATCA AGTGGTTGGC GCTCGGCTTC AAAGTGTACT TCACCAACGC GTGGTGTTGG 
CTCGATTTCG TGATTGTCAT GGTATCGCTT ATCAACTTCG TTGCTTCACT TGTTGGAGCT 
GGTGGTATTC AAGCCTTCAA GACTATGCGA ACGTTAAGAG CACTGAGACC ACTACGTGCC 
ATGTCCCGTA TGCAGGGCAT GAGGGTCGTC GTTAATGCGC TGGTACAAGC TATACCGTCC 
ATCTTCAATG TGCTATTGGT GTGTCTAATA TTTTGGCTAA TTTTTGCCAT AATGGGTGTA 
CAGCTTTTTG CTGGAAAATA TTTTAAGTGC GAGGACATGA ATGGCACGAA GCTCAGCCAC 
GAGATCATAC CAAATCGCAA TGCCTGCGAG AGCGAGAACT ACACGTGGGT GAATTCAGCA 
ATGAATTTCG ATCATGTAGG TAACGCGTAT CTGTGCCTTT TCCAAGTGGC CACCTTCAAA 
GGCTGGATAC AAATCATGAA CGATGCTATC GATTCACGAG AGGTGGACAA GCAACCAATT 
CGTGAAACGA ACATCTACAT GTATTTATAT TTCGTATTCT TCATCATATT TGGATCATTT 
TTCACACTCA ATCTGTTCAT TGGTGTTATC ATTGATAATT TTAATGAGCA AAAGAAAAAA 
GCAGGTGGAT CATTAGAAAT GTTCATGACA GAAGATCAGA AAAAGTACTA TAATGCTATG 
AAAAAGATGG GCTCTAAAAA ACCATTAAAA GCCATTCCAA GACCAAGGTG GCGACCACAA 
GCAATAGTCT TTGAAATAGT AACCGATAAG AAATTCGATA TAATCATTAT GTTATTCATT 
GGTCTGAACA TGTTCACCAT GACCCTCGAT CGTTACGATG CGTCGGACAC GTATAACGCG 
GTCCTAGACT ATCTCAATGC GATATTCGTA GTTATTTTCA GTTCCGAATG TCTATTAAAA 
ATATTCGCTT TACGATATCA CTATTTTATT GAGCCATGGA ATTTATTTGA TGTAGTAGTT 
GTCATTTTAT CCATCTTAGG TCTTGTACTT AGCGATATTA TCGAGAAGTA CTTCGTGTCG 
CCGACCCTGC TCCGAGTGGT GCGTGTGGCG AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG 
GGAGCCAAGG GCATTCGGAC ACTGCTCTTC GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC 
AACATCTGCC TGCTGCTGTT CCTGGTCATG TTCATCTTTG CCATTTTCGG CATGTCGTTC 
TTCATGCACG TGAAGGAGAA GAGCGGCATC AACGACGTCT ACAACTTCAA GACCTTTGGC 
CAGAGCATGA TCCTGCTCTT TCAGATGTCG ACGTCAGCCG GTTGGGATGG TGTACTGGAC 
GCCATTATCA ATGAGGAAGC ATGCGATCCA CCCGACAACG ACAAAGGCTA TCCGGGCAAT 
TGTGGTTCAG CGACCGTTGG AATAACGTTT CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG 
ATAGTTATTA ATATGTACAT TGCTGTCATT CTCGAGAACT ATAGTCAGGC CACCGAGGAC 
GTGCAAGAGG GTCTAACCGA CGACGACTAC GACATGTACT ATGAGATCTG GCAGCAATTC 
GATCCGGAGG GCACCCAGTA CATACGCTAT GATCAGCTGT CCGAATTCCT GGACGTACTG 
GAGCCCCCGC TGCAGATCCA CAAACCGAAC AAGTACAAGA TCATATCGAT GGACATACCC 
ATCTGTCGCG GTGACCTCAT GTACTGCGTC GACATCCTCG ACGCCCTTAC GAAAGACTTC 
TTTGCGCGGA AGGGCAATCC GATAGAGGAG ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC 
CCGGATACGG AGGGCTACGA GCCCGTCTCA TCAACGCTGT GGCGTCAGCG TGAGGAGTAC 
TGCGCCCGGC TAATCCAGCA CGCCTGGCGA AAGCACAAGG CGCGCGGCGA GGGAGGTGGG 
TCCTTTGAGC CGGATACGGA TCATGGCGAT GGCGGTGATC CGGATGCCGG GGACCCGGCG 
CCCGATGAAG CAACGGACGG CGATGCGCCC GCTGGTGGAG ATGGTAGTGT TAACGGTACT 
GCAGAAGGAG CTGCCGATGC CGATGAGAGT AATGTAAATA GTCCGGGTGA GGATGCAGCG 
GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG GCGGCGGGCA CGACGACGGC GGGAAGTCCC 
GGAGCGGGTA GCGCCGGGCG ACAGACCGCC GTTCTCGTGG AGAGCGACGG GTTCGTGACG 
AAGAACGGCC ACAAGGTGGT CATCCACTCG CGATCGCCGA GCATCACGTC GCGCACGGCG 
GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201705.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188634.1 Drosophila melanogaster paralytic (para), transcript variant AM, mRNA.

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CloneID	OFa03651
Clone ID Related Accession (Same CDS sequence)	NM_001201705.1
Accession Version	NM_001201705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AM
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGACA 
TCCTTATCCT TACCTGGTTC ACCGTTTAAC ATACGCAGGG GATCACGTAG TTCTCACAAG 
TACACGATAC GGAACGGACG TGGCCGCTTT GGTATACCCG GTAGCGATCG TAAGCCATTG 
GTATTGTCAA CATATCAGGA TGCCCAGCAG CACTTGCCCT ATGCCGACGA CTCGAATGCC 
GTCACCCCGA TGTCCGAAGA GAATGGGGCC ATCATAGTGC CCGTGTACTA TGGCAATCTA 
GGCTCCCGAC ACTCATCGTA TACCTCGCAT CAGTCCCGAA TATCGTATAC CTCACATGGC 
GATCTACTCG GCGGCATGGC CGTCATGGGC GTCAGCACAA TGACCAAGGA GAGCAAATTG 
CGCAACCGGA ACACACGCAA TCAATCAGTG GGCGCCACCA ATGGCGGCAC CACCTGTCTG 
GACACCAATC ACAAGCTCGA TCATCGCGAC TACGAAATTG GCCTGGAGTG CACGGACGAA 
GCTGGCAAGA TTAAACATCA TGACAATCCT TTTATCGAGC CCGTCCAGAC ACAAACGGTG 
GTTGATATGA AAGATGTGAT GGTCCTGAAT GACATCATCG AACAGGCCGC TGGTCGGCAC 
AGTCGGGCAA GCGATCGCGG TGTCTCCGTT TACTATTTCC CAACAGAGGA CGATGACGAG 
GATGGGCCGA CGTTCAAAGA CAAGGCACTC GAAGTGATCC TCAAAGGCAT CGATGTGTTT 
TGTGTGTGGG ACTGTTGCTG GGTTTGGTTG AAATTTCAGG AGTGGGTATC GCTCATCGTC 
TTCGATCCCT TCGTCGAGCT CTTCATCACG CTGTGCATTG TGGTCAACAC GATGTTCATG 
GCAATGGATC ACCACGATAT GAACAAGGAG ATGGAACGCG TGCTCAAGAG TGGCAACTAT 
TTCTTCACCG CCACCTTTGC CATCGAGGCC ACCATGAAGC TAATGGCCAT GAGCCCCAAG 
TACTATTTCC AGGAGGGCTG GAACATCTTC GACTTCATTA TCGTGGCCCT ATCGCTATTG 
GAACTGGGAC TCGAGGGTGT CCAGGGTCTG TCCGTATTGC GTTCCTTTCG ATTGCTGCGT 
GTATTCAAAC TGGCCAAGTC TTGGCCCACA CTTAATTTAC TCATTTCGAT TATGGGACGC 
ACCATGGGCG CTTTGGGTAA TCTGACATTT GTACTTTGCA TTATCATCTT CATCTTTGCG 
GTGATGGGAA TGCAACTGTT CGGAAAGAAT TATCATGATC ACAAGGACCG CTTTCCGGAT 
GGCGACCTGC CGCGCTGGAA CTTCACCGAC TTTATGCACA GCTTCATGAT CGTGTTCCGG 
GTGCTCTGCG GAGAATGGAT CGAGTCCATG TGGGACTGCA TGTACGTGGG CGATGTCTCG 
TGCATTCCCT TCTTCTTGGC CACCGTTGTC ATCGGCAATC TTGTGGTACT TAACCTTTTC 
TTAGCCTTGC TTTTGTCCAA TTTTGGCTCA TCTAGCTTAT CAGCGCCGAC TGCCGATAAC 
GATACGAATA AAATAGCCGA GGCCTTCAAT CGAATTGGCC GATTTAAAAG TTGGGTTAAG 
CGTAATATTG CTGATTGTTT CAAGTTAATA CGTAACAAAT TGACAAATCA AATAAGTGAT 
CAACCATCAG GCAAAGGCGT TTGTCGTTGT ATATCTGCAG AGCATGGTGA CAACGAACTG 
GAGCTGGGCC ACGACGAGAT CCTCGCCGAC GGCCTCATCA AGAAGGGGAT CAAGGAGCAG 
ACGCAACTGG AGGTGGCCAT CGGGGATGGC ATGGAATTCA CGATACACGG CGACATGAAG 
AACAACAAGC CGAAGAAATC CAAATATCTA AATAACGCAA CGGACGACGA CACTGCCAGC 
ATTAACTCAT ATGGTAGCCA TAAGAATCGA CCATTCAAGG ACGAGAGCCA CAAGGGCAGC 
GCCGAGACGA TGGAGGGCGA GGAGAAGCGC GACGCCAGCA AGGAGGATTT AGGTCTCGAC 
GAGGAACTGG ACGAGGAGGG CGAATGCGAG GAGGGCCCGC TCGACGGTGA TATCATTATT 
CATGCACACG ACGAGGATAT ACTCGATGAA TATCCAGCTG ATTGCTGCCC CGATTCGTAC 
TATAAGAAAT TTCCGATCTT AGCCGGTGAC GATGACTCGC CGTTCTGGCA AGGATGGGGC 
AATTTACGAC TGAAAACTTT TCAATTAATT GAAAATAAAT ATTTTGAAAC AGCTGTTATC 
ACTATGATTT TAATGAGTAG CTTAGCTTTG GCATTAGAAG ATGTACATCT GCCACAAAGA 
CCCATACTGC AGGATATTTT ATACTATATG GACAGAATAT TTACGGTTAT ATTCTTCTTG 
GAAATGTTAA TCAAGTGGTT GGCGCTCGGC TTCAAAGTGT ACTTCACCAA CGCGTGGTGT 
TGGCTCGATT TCGTGATTGT CATGGTATCG CTTATCAACT TCGTTGCTTC ACTTGTTGGA 
GCTGGTGGTA TTCAAGCCTT CAAGACTATG CGAACGTTAA GAGCACTGAG ACCACTACGT 
GCCATGTCCC GTATGCAGGG CATGAGGGTC GTCGTTAATG CGCTGGTACA AGCTATACCG 
TCCATCTTCA ATGTGCTATT GGTGTGTCTA ATATTTTGGC TAATTTTTGC CATAATGGGT 
GTACAGCTTT TTGCTGGAAA ATATTTTAAG TGCGAGGACA TGAATGGCAC GAAGCTCAGC 
CACGAGATCA TACCAAATCG CAATGCCTGC GAGAGCGAGA ACTACACGTG GGTGAATTCA 
GCAATGAATT TCGATCATGT AGGTAACGCG TATCTGTGCC TTTTCCAAGT GGCCACCTTC 
AAAGGCTGGA TACAAATCAT GAACGATGCT ATCGATTCAC GAGAGGTGGA CAAGCAACCA 
ATTCGTGAAA CGAACATCTA CATGTATTTA TATTTCGTAT TCTTCATCAT ATTTGGATCA 
TTTTTCACAC TCAATCTGTT CATTGGTGTT ATCATTGATA ATTTTAATGA GCAAAAGAAA 
AAAGCAGGTG GATCATTAGA AATGTTCATG ACAGAAGATC AGAAAAAGTA CTATAATGCT 
ATGAAAAAGA TGGGCTCTAA AAAACCATTA AAAGCCATTC CAAGACCAAG GTGGCGACCA 
CAAGCAATAG TCTTTGAAAT AGTAACCGAT AAGAAATTCG ATATAATCAT TATGTTATTC 
ATTGGTCTGA ACATGTTCAC CATGACCCTC GATCGTTACG ATGCGTCGGA CACGTATAAC 
GCGGTCCTAG ACTATCTCAA TGCGATATTC GTAGTTATTT TCAGTTCCGA ATGTCTATTA 
AAAATATTCG CTTTACGATA TCACTATTTT ATTGAGCCAT GGAATTTATT TGATGTAGTA 
GTTGTCATTT TATCCATCTT AGGTCTTGTA CTTAGCGATA TTATCGAGAA GTACTTCGTG 
TCGCCGACCC TGCTCCGAGT GGTGCGTGTG GCGAAAGTGG GCCGTGTCCT TCGACTGGTG 
AAGGGAGCCA AGGGCATTCG GACACTGCTC TTCGCGTTGG CCATGTCGCT GCCGGCCCTG 
TTCAACATCT GCCTGCTGCT GTTCCTGGTC ATGTTCATCT TTGCCATTTT CGGCATGTCG 
TTCTTCATGC ACGTGAAGGA GAAGAGCGGC ATCAACGACG TCTACAACTT CAAGACCTTT 
GGCCAGAGCA TGATCCTGCT CTTTCAGATG TCGACGTCAG CCGGTTGGGA TGGTGTACTG 
GACGCCATTA TCAATGAGGA AGCATGCGAT CCACCCGACA ACGACAAAGG CTATCCGGGC 
AATTGTGGTT CAGCGACCGT TGGAATAACG TTTCTCCTCT CATACCTAGT TATAAGCTTT 
TTGATAGTTA TTAATATGTA CATTGCTGTC ATTCTCGAGA ACTATAGTCA GGCCACCGAG 
GACGTGCAAG AGGGTCTAAC CGACGACGAC TACGACATGT ACTATGAGAT CTGGCAGCAA 
TTCGATCCGG AGGGCACCCA GTACATACGC TATGATCAGC TGTCCGAATT CCTGGACGTA 
CTGGAGCCCC CGCTGCAGAT CCACAAACCG AACAAGTACA AGATCATATC GATGGACATA 
CCCATCTGTC GCGGTGACCT CATGTACTGC GTCGACATCC TCGACGCCCT TACGAAAGAC 
TTCTTTGCGC GGAAGGGCAA TCCGATAGAG GAGACGGGTG AGATTGGTGA GATAGCGGCC 
CGCCCGGATA CGGAGGGCTA CGAGCCCGTC TCATCAACGC TGTGGCGTCA GCGTGAGGAG 
TACTGCGCCC GGCTAATCCA GCACGCCTGG CGAAAGCACA AGGCGCGCGG CGAGGGAGGT 
GGGTCCTTTG AGCCGGATAC GGATCATGGC GATGGCGGTG ATCCGGATGC CGGGGACCCG 
GCGCCCGATG AAGCAACGGA CGGCGATGCG CCCGCTGGTG GAGATGGTAG TGTTAACGGT 
ACTGCAGAAG GAGCTGCCGA TGCCGATGAG AGTAATGTAA ATAGTCCGGG TGAGGATGCA 
GCGGCGGCGG CAGCAGCAGC AGCAGCAGCG GCGGCGGCGG GCACGACGAC GGCGGGAAGT 
CCCGGAGCGG GTAGCGCCGG GCGACAGACC GCCGTTCTCG TGGAGAGCGA CGGGTTCGTG 
ACGAAGAACG GCCACAAGGT GGTCATCCAC TCGCGATCGC CGAGCATCAC GTCGCGCACG 
GCGGATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188634.1
CDS	269..6520
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201705.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AM, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201705.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGACA 
TCCTTATCCT TACCTGGTTC ACCGTTTAAC ATACGCAGGG GATCACGTAG TTCTCACAAG 
TACACGATAC GGAACGGACG TGGCCGCTTT GGTATACCCG GTAGCGATCG TAAGCCATTG 
GTATTGTCAA CATATCAGGA TGCCCAGCAG CACTTGCCCT ATGCCGACGA CTCGAATGCC 
GTCACCCCGA TGTCCGAAGA GAATGGGGCC ATCATAGTGC CCGTGTACTA TGGCAATCTA 
GGCTCCCGAC ACTCATCGTA TACCTCGCAT CAGTCCCGAA TATCGTATAC CTCACATGGC 
GATCTACTCG GCGGCATGGC CGTCATGGGC GTCAGCACAA TGACCAAGGA GAGCAAATTG 
CGCAACCGGA ACACACGCAA TCAATCAGTG GGCGCCACCA ATGGCGGCAC CACCTGTCTG 
GACACCAATC ACAAGCTCGA TCATCGCGAC TACGAAATTG GCCTGGAGTG CACGGACGAA 
GCTGGCAAGA TTAAACATCA TGACAATCCT TTTATCGAGC CCGTCCAGAC ACAAACGGTG 
GTTGATATGA AAGATGTGAT GGTCCTGAAT GACATCATCG AACAGGCCGC TGGTCGGCAC 
AGTCGGGCAA GCGATCGCGG TGTCTCCGTT TACTATTTCC CAACAGAGGA CGATGACGAG 
GATGGGCCGA CGTTCAAAGA CAAGGCACTC GAAGTGATCC TCAAAGGCAT CGATGTGTTT 
TGTGTGTGGG ACTGTTGCTG GGTTTGGTTG AAATTTCAGG AGTGGGTATC GCTCATCGTC 
TTCGATCCCT TCGTCGAGCT CTTCATCACG CTGTGCATTG TGGTCAACAC GATGTTCATG 
GCAATGGATC ACCACGATAT GAACAAGGAG ATGGAACGCG TGCTCAAGAG TGGCAACTAT 
TTCTTCACCG CCACCTTTGC CATCGAGGCC ACCATGAAGC TAATGGCCAT GAGCCCCAAG 
TACTATTTCC AGGAGGGCTG GAACATCTTC GACTTCATTA TCGTGGCCCT ATCGCTATTG 
GAACTGGGAC TCGAGGGTGT CCAGGGTCTG TCCGTATTGC GTTCCTTTCG ATTGCTGCGT 
GTATTCAAAC TGGCCAAGTC TTGGCCCACA CTTAATTTAC TCATTTCGAT TATGGGACGC 
ACCATGGGCG CTTTGGGTAA TCTGACATTT GTACTTTGCA TTATCATCTT CATCTTTGCG 
GTGATGGGAA TGCAACTGTT CGGAAAGAAT TATCATGATC ACAAGGACCG CTTTCCGGAT 
GGCGACCTGC CGCGCTGGAA CTTCACCGAC TTTATGCACA GCTTCATGAT CGTGTTCCGG 
GTGCTCTGCG GAGAATGGAT CGAGTCCATG TGGGACTGCA TGTACGTGGG CGATGTCTCG 
TGCATTCCCT TCTTCTTGGC CACCGTTGTC ATCGGCAATC TTGTGGTACT TAACCTTTTC 
TTAGCCTTGC TTTTGTCCAA TTTTGGCTCA TCTAGCTTAT CAGCGCCGAC TGCCGATAAC 
GATACGAATA AAATAGCCGA GGCCTTCAAT CGAATTGGCC GATTTAAAAG TTGGGTTAAG 
CGTAATATTG CTGATTGTTT CAAGTTAATA CGTAACAAAT TGACAAATCA AATAAGTGAT 
CAACCATCAG GCAAAGGCGT TTGTCGTTGT ATATCTGCAG AGCATGGTGA CAACGAACTG 
GAGCTGGGCC ACGACGAGAT CCTCGCCGAC GGCCTCATCA AGAAGGGGAT CAAGGAGCAG 
ACGCAACTGG AGGTGGCCAT CGGGGATGGC ATGGAATTCA CGATACACGG CGACATGAAG 
AACAACAAGC CGAAGAAATC CAAATATCTA AATAACGCAA CGGACGACGA CACTGCCAGC 
ATTAACTCAT ATGGTAGCCA TAAGAATCGA CCATTCAAGG ACGAGAGCCA CAAGGGCAGC 
GCCGAGACGA TGGAGGGCGA GGAGAAGCGC GACGCCAGCA AGGAGGATTT AGGTCTCGAC 
GAGGAACTGG ACGAGGAGGG CGAATGCGAG GAGGGCCCGC TCGACGGTGA TATCATTATT 
CATGCACACG ACGAGGATAT ACTCGATGAA TATCCAGCTG ATTGCTGCCC CGATTCGTAC 
TATAAGAAAT TTCCGATCTT AGCCGGTGAC GATGACTCGC CGTTCTGGCA AGGATGGGGC 
AATTTACGAC TGAAAACTTT TCAATTAATT GAAAATAAAT ATTTTGAAAC AGCTGTTATC 
ACTATGATTT TAATGAGTAG CTTAGCTTTG GCATTAGAAG ATGTACATCT GCCACAAAGA 
CCCATACTGC AGGATATTTT ATACTATATG GACAGAATAT TTACGGTTAT ATTCTTCTTG 
GAAATGTTAA TCAAGTGGTT GGCGCTCGGC TTCAAAGTGT ACTTCACCAA CGCGTGGTGT 
TGGCTCGATT TCGTGATTGT CATGGTATCG CTTATCAACT TCGTTGCTTC ACTTGTTGGA 
GCTGGTGGTA TTCAAGCCTT CAAGACTATG CGAACGTTAA GAGCACTGAG ACCACTACGT 
GCCATGTCCC GTATGCAGGG CATGAGGGTC GTCGTTAATG CGCTGGTACA AGCTATACCG 
TCCATCTTCA ATGTGCTATT GGTGTGTCTA ATATTTTGGC TAATTTTTGC CATAATGGGT 
GTACAGCTTT TTGCTGGAAA ATATTTTAAG TGCGAGGACA TGAATGGCAC GAAGCTCAGC 
CACGAGATCA TACCAAATCG CAATGCCTGC GAGAGCGAGA ACTACACGTG GGTGAATTCA 
GCAATGAATT TCGATCATGT AGGTAACGCG TATCTGTGCC TTTTCCAAGT GGCCACCTTC 
AAAGGCTGGA TACAAATCAT GAACGATGCT ATCGATTCAC GAGAGGTGGA CAAGCAACCA 
ATTCGTGAAA CGAACATCTA CATGTATTTA TATTTCGTAT TCTTCATCAT ATTTGGATCA 
TTTTTCACAC TCAATCTGTT CATTGGTGTT ATCATTGATA ATTTTAATGA GCAAAAGAAA 
AAAGCAGGTG GATCATTAGA AATGTTCATG ACAGAAGATC AGAAAAAGTA CTATAATGCT 
ATGAAAAAGA TGGGCTCTAA AAAACCATTA AAAGCCATTC CAAGACCAAG GTGGCGACCA 
CAAGCAATAG TCTTTGAAAT AGTAACCGAT AAGAAATTCG ATATAATCAT TATGTTATTC 
ATTGGTCTGA ACATGTTCAC CATGACCCTC GATCGTTACG ATGCGTCGGA CACGTATAAC 
GCGGTCCTAG ACTATCTCAA TGCGATATTC GTAGTTATTT TCAGTTCCGA ATGTCTATTA 
AAAATATTCG CTTTACGATA TCACTATTTT ATTGAGCCAT GGAATTTATT TGATGTAGTA 
GTTGTCATTT TATCCATCTT AGGTCTTGTA CTTAGCGATA TTATCGAGAA GTACTTCGTG 
TCGCCGACCC TGCTCCGAGT GGTGCGTGTG GCGAAAGTGG GCCGTGTCCT TCGACTGGTG 
AAGGGAGCCA AGGGCATTCG GACACTGCTC TTCGCGTTGG CCATGTCGCT GCCGGCCCTG 
TTCAACATCT GCCTGCTGCT GTTCCTGGTC ATGTTCATCT TTGCCATTTT CGGCATGTCG 
TTCTTCATGC ACGTGAAGGA GAAGAGCGGC ATCAACGACG TCTACAACTT CAAGACCTTT 
GGCCAGAGCA TGATCCTGCT CTTTCAGATG TCGACGTCAG CCGGTTGGGA TGGTGTACTG 
GACGCCATTA TCAATGAGGA AGCATGCGAT CCACCCGACA ACGACAAAGG CTATCCGGGC 
AATTGTGGTT CAGCGACCGT TGGAATAACG TTTCTCCTCT CATACCTAGT TATAAGCTTT 
TTGATAGTTA TTAATATGTA CATTGCTGTC ATTCTCGAGA ACTATAGTCA GGCCACCGAG 
GACGTGCAAG AGGGTCTAAC CGACGACGAC TACGACATGT ACTATGAGAT CTGGCAGCAA 
TTCGATCCGG AGGGCACCCA GTACATACGC TATGATCAGC TGTCCGAATT CCTGGACGTA 
CTGGAGCCCC CGCTGCAGAT CCACAAACCG AACAAGTACA AGATCATATC GATGGACATA 
CCCATCTGTC GCGGTGACCT CATGTACTGC GTCGACATCC TCGACGCCCT TACGAAAGAC 
TTCTTTGCGC GGAAGGGCAA TCCGATAGAG GAGACGGGTG AGATTGGTGA GATAGCGGCC 
CGCCCGGATA CGGAGGGCTA CGAGCCCGTC TCATCAACGC TGTGGCGTCA GCGTGAGGAG 
TACTGCGCCC GGCTAATCCA GCACGCCTGG CGAAAGCACA AGGCGCGCGG CGAGGGAGGT 
GGGTCCTTTG AGCCGGATAC GGATCATGGC GATGGCGGTG ATCCGGATGC CGGGGACCCG 
GCGCCCGATG AAGCAACGGA CGGCGATGCG CCCGCTGGTG GAGATGGTAG TGTTAACGGT 
ACTGCAGAAG GAGCTGCCGA TGCCGATGAG AGTAATGTAA ATAGTCCGGG TGAGGATGCA 
GCGGCGGCGG CAGCAGCAGC AGCAGCAGCG GCGGCGGCGG GCACGACGAC GGCGGGAAGT 
CCCGGAGCGG GTAGCGCCGG GCGACAGACC GCCGTTCTCG TGGAGAGCGA CGGGTTCGTG 
ACGAAGAACG GCCACAAGGT GGTCATCCAC TCGCGATCGC CGAGCATCAC GTCGCGCACG 
GCGGATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201701.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188630.1 Drosophila melanogaster paralytic (para), transcript variant AI, mRNA.

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CloneID	OFa03652
Clone ID Related Accession (Same CDS sequence)	NM_001201701.1
Accession Version	NM_001201701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AI
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG CAAAGGCGTT TGTCGTTGTA TATCTGCAGA GCATGGTGAC 
AACGAACTGG AGCTGGGCCA CGACGAGATC CTCGCCGACG GCCTCATCAA GAAGGGGATC 
AAGGAGCAGA CGCAACTGGA GGTGGCCATC GGGGATGGCA TGGAATTCAC GATACACGGC 
GACATGAAGA ACAACAAGCC GAAGAAATCC AAATATCTAA ATAACGCAAC GGACGACGAC 
ACTGCCAGCA TTAACTCATA TGGTAGCCAT AAGAATCGAC CATTCAAGGA CGAGAGCCAC 
AAGGGCAGCG CCGAGACGAT GGAGGGCGAG GAGAAGCGCG ACGCCAGCAA GGAGGATTTA 
GGTCTCGACG AGGAACTGGA CGAGGAGGGC GAATGCGAGG AGGGCCCGCT CGACGGTGAT 
ATCATTATTC ATGCACACGA CGAGGATATA CTCGATGAAT ATCCAGCTGA TTGCTGCCCC 
GATTCGTACT ATAAGAAATT TCCGATCTTA GCCGGTGACG ATGACTCGCC GTTCTGGCAA 
GGATGGGGCA ATTTACGACT GAAAACTTTT CAATTAATTG AAAATAAATA TTTTGAAACA 
GCTGTTATCA CTATGATTTT AATGAGTAGC TTAGCTTTGG CATTAGAAGA TGTACATCTG 
CCACAAAGAC CCATACTGCA GGATATTTTA TACTATATGG ACAGAATATT TACGGTTATA 
TTCTTCTTGG AAATGTTAAT CAAGTGGTTG GCGCTCGGCT TCAAAGTGTA CTTCACCAAC 
GCGTGGTGTT GGCTCGATTT CGTGATTGTC ATGGTATCGC TTATCAACTT CGTTGCTTCA 
CTTGTTGGAG CTGGTGGTAT TCAAGCCTTC AAGACTATGC GAACGTTAAG AGCACTGAGA 
CCACTACGTG CCATGTCCCG TATGCAGGGC ATGAGGGTCG TCGTTAATGC GCTGGTACAA 
GCTATACCGT CCATCTTCAA TGTGCTATTG GTGTGTCTAA TATTTTGGCT AATTTTTGCC 
ATAATGGGTG TACAGCTTTT TGCTGGAAAA TATTTTAAGT GCGAGGACAT GAATGGCACG 
AAGCTCAGCC ACGAGATCAT ACCAAATCGC AATGCCTGCG AGAGCGAGAA CTACACGTGG 
GTGAATTCAG CAATGAATTT CGATCATGTA GGTAACGCGT ATCTGTGCCT TTTCCAAGTG 
GCCACCTTCA AAGGCTGGAT ACAAATCATG AACGATGCTA TCGATTCACG AGAGGTGGAC 
AAGCAACCAA TTCGTGAAAC GAACATCTAC ATGTATTTAT ATTTCGTATT CTTCATCATA 
TTTGGATCAT TTTTCACACT CAATCTGTTC ATTGGTGTTA TCATTGATAA TTTTAATGAG 
CAAAAGAAAA AAGCAGGTGG ATCATTAGAA ATGTTCATGA CAGAAGATCA GAAAAAGTAC 
TATAATGCTA TGAAAAAGAT GGGCTCTAAA AAACCATTAA AAGCCATTCC AAGACCAAGG 
TGGCGACCAC AAGCAATAGT CTTTGAAATA GTAACCGATA AGAAATTCGA TATAATCATT 
ATGTTATTCA TTGGTCTGAA CATGTTCACC ATGACCCTCG ATCGTTACGA TGCGTCGGAC 
ACGTATAACG CGGTCCTAGA CTATCTCAAT GCGATATTCG TAGTTATTTT CAGTTCCGAA 
TGTCTATTAA AAATATTCGC TTTACGATAT CACTATTTTA TTGAGCCATG GAATTTATTT 
GATGTAGTAG TTGTCATTTT ATCCATCTTA GGTCTTGTAC TTAGCGATAT TATCGAGAAG 
TACTTCGTGT CGCCGACCCT GCTCCGAGTG GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT 
CGACTGGTGA AGGGAGCCAA GGGCATTCGG ACACTGCTCT TCGCGTTGGC CATGTCGCTG 
CCGGCCCTGT TCAACATCTG CCTGCTGCTG TTCCTGGTCA TGTTCATCTT TGCCATTTTC 
GGCATGTCGT TCTTCATGCA CGTGAAGGAG AAGAGCGGCA TCAACGACGT CTACAACTTC 
AAGACCTTTG GCCAGAGCAT GATCCTGCTC TTTCAGATGT CGACGTCAGC CGGTTGGGAT 
GGTGTACTGG ACGCCATTAT CAATGAGGAA GCATGCGATC CACCCGACAA CGACAAAGGC 
TATCCGGGCA ATTGTGGTTC AGCGACCGTT GGAATAACGT TTCTCCTCTC ATACCTAGTT 
ATAAGCTTTT TGATAGTTAT TAATATGTAC ATTGCTGTCA TTCTCGAGAA CTATAGTCAG 
GCCACCGAGG ACGTGCAAGA GGGTCTAACC GACGACGACT ACGACATGTA CTATGAGATC 
TGGCAGCAAT TCGATCCGGA GGGCACCCAG TACATACGCT ATGATCAGCT GTCCGAATTC 
CTGGACGTAC TGGAGCCCCC GCTGCAGATC CACAAACCGA ACAAGTACAA GATCATATCG 
ATGGACATAC CCATCTGTCG CGGTGACCTC ATGTACTGCG TCGACATCCT CGACGCCCTT 
ACGAAAGACT TCTTTGCGCG GAAGGGCAAT CCGATAGAGG AGACGGGTGA GATTGGTGAG 
ATAGCGGCCC GCCCGGATAC GGAGGGCTAC GAGCCCGTCT CATCAACGCT GTGGCGTCAG 
CGTGAGGAGT ACTGCGCCCG GCTAATCCAG CACGCCTGGC GAAAGCACAA GGCGCGCGGC 
GAGGGAGGTG GGTCCTTTGA GCCGGATACG GATCATGGCG ATGGCGGTGA TCCGGATGCC 
GGGGACCCGG CGCCCGATGA AGCAACGGAC GGCGATGCGC CCGCTGGTGG AGATGGTAGT 
GTTAACGGTA CTGCAGAAGG AGCTGCCGAT GCCGATGAGA GTAATGTAAA TAGTCCGGGT 
GAGGATGCAG CGGCGGCGGC AGCAGCAGCA GCAGCAGCGG CGGCGGCGGG CACGACGACG 
GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG CGACAGACCG CCGTTCTCGT GGAGAGCGAC 
GGGTTCGTGA CGAAGAACGG CCACAAGGTG GTCATCCACT CGCGATCGCC GAGCATCACG 
TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188630.1
CDS	269..6529
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201701.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AI, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201701.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG CAAAGGCGTT TGTCGTTGTA TATCTGCAGA GCATGGTGAC 
AACGAACTGG AGCTGGGCCA CGACGAGATC CTCGCCGACG GCCTCATCAA GAAGGGGATC 
AAGGAGCAGA CGCAACTGGA GGTGGCCATC GGGGATGGCA TGGAATTCAC GATACACGGC 
GACATGAAGA ACAACAAGCC GAAGAAATCC AAATATCTAA ATAACGCAAC GGACGACGAC 
ACTGCCAGCA TTAACTCATA TGGTAGCCAT AAGAATCGAC CATTCAAGGA CGAGAGCCAC 
AAGGGCAGCG CCGAGACGAT GGAGGGCGAG GAGAAGCGCG ACGCCAGCAA GGAGGATTTA 
GGTCTCGACG AGGAACTGGA CGAGGAGGGC GAATGCGAGG AGGGCCCGCT CGACGGTGAT 
ATCATTATTC ATGCACACGA CGAGGATATA CTCGATGAAT ATCCAGCTGA TTGCTGCCCC 
GATTCGTACT ATAAGAAATT TCCGATCTTA GCCGGTGACG ATGACTCGCC GTTCTGGCAA 
GGATGGGGCA ATTTACGACT GAAAACTTTT CAATTAATTG AAAATAAATA TTTTGAAACA 
GCTGTTATCA CTATGATTTT AATGAGTAGC TTAGCTTTGG CATTAGAAGA TGTACATCTG 
CCACAAAGAC CCATACTGCA GGATATTTTA TACTATATGG ACAGAATATT TACGGTTATA 
TTCTTCTTGG AAATGTTAAT CAAGTGGTTG GCGCTCGGCT TCAAAGTGTA CTTCACCAAC 
GCGTGGTGTT GGCTCGATTT CGTGATTGTC ATGGTATCGC TTATCAACTT CGTTGCTTCA 
CTTGTTGGAG CTGGTGGTAT TCAAGCCTTC AAGACTATGC GAACGTTAAG AGCACTGAGA 
CCACTACGTG CCATGTCCCG TATGCAGGGC ATGAGGGTCG TCGTTAATGC GCTGGTACAA 
GCTATACCGT CCATCTTCAA TGTGCTATTG GTGTGTCTAA TATTTTGGCT AATTTTTGCC 
ATAATGGGTG TACAGCTTTT TGCTGGAAAA TATTTTAAGT GCGAGGACAT GAATGGCACG 
AAGCTCAGCC ACGAGATCAT ACCAAATCGC AATGCCTGCG AGAGCGAGAA CTACACGTGG 
GTGAATTCAG CAATGAATTT CGATCATGTA GGTAACGCGT ATCTGTGCCT TTTCCAAGTG 
GCCACCTTCA AAGGCTGGAT ACAAATCATG AACGATGCTA TCGATTCACG AGAGGTGGAC 
AAGCAACCAA TTCGTGAAAC GAACATCTAC ATGTATTTAT ATTTCGTATT CTTCATCATA 
TTTGGATCAT TTTTCACACT CAATCTGTTC ATTGGTGTTA TCATTGATAA TTTTAATGAG 
CAAAAGAAAA AAGCAGGTGG ATCATTAGAA ATGTTCATGA CAGAAGATCA GAAAAAGTAC 
TATAATGCTA TGAAAAAGAT GGGCTCTAAA AAACCATTAA AAGCCATTCC AAGACCAAGG 
TGGCGACCAC AAGCAATAGT CTTTGAAATA GTAACCGATA AGAAATTCGA TATAATCATT 
ATGTTATTCA TTGGTCTGAA CATGTTCACC ATGACCCTCG ATCGTTACGA TGCGTCGGAC 
ACGTATAACG CGGTCCTAGA CTATCTCAAT GCGATATTCG TAGTTATTTT CAGTTCCGAA 
TGTCTATTAA AAATATTCGC TTTACGATAT CACTATTTTA TTGAGCCATG GAATTTATTT 
GATGTAGTAG TTGTCATTTT ATCCATCTTA GGTCTTGTAC TTAGCGATAT TATCGAGAAG 
TACTTCGTGT CGCCGACCCT GCTCCGAGTG GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT 
CGACTGGTGA AGGGAGCCAA GGGCATTCGG ACACTGCTCT TCGCGTTGGC CATGTCGCTG 
CCGGCCCTGT TCAACATCTG CCTGCTGCTG TTCCTGGTCA TGTTCATCTT TGCCATTTTC 
GGCATGTCGT TCTTCATGCA CGTGAAGGAG AAGAGCGGCA TCAACGACGT CTACAACTTC 
AAGACCTTTG GCCAGAGCAT GATCCTGCTC TTTCAGATGT CGACGTCAGC CGGTTGGGAT 
GGTGTACTGG ACGCCATTAT CAATGAGGAA GCATGCGATC CACCCGACAA CGACAAAGGC 
TATCCGGGCA ATTGTGGTTC AGCGACCGTT GGAATAACGT TTCTCCTCTC ATACCTAGTT 
ATAAGCTTTT TGATAGTTAT TAATATGTAC ATTGCTGTCA TTCTCGAGAA CTATAGTCAG 
GCCACCGAGG ACGTGCAAGA GGGTCTAACC GACGACGACT ACGACATGTA CTATGAGATC 
TGGCAGCAAT TCGATCCGGA GGGCACCCAG TACATACGCT ATGATCAGCT GTCCGAATTC 
CTGGACGTAC TGGAGCCCCC GCTGCAGATC CACAAACCGA ACAAGTACAA GATCATATCG 
ATGGACATAC CCATCTGTCG CGGTGACCTC ATGTACTGCG TCGACATCCT CGACGCCCTT 
ACGAAAGACT TCTTTGCGCG GAAGGGCAAT CCGATAGAGG AGACGGGTGA GATTGGTGAG 
ATAGCGGCCC GCCCGGATAC GGAGGGCTAC GAGCCCGTCT CATCAACGCT GTGGCGTCAG 
CGTGAGGAGT ACTGCGCCCG GCTAATCCAG CACGCCTGGC GAAAGCACAA GGCGCGCGGC 
GAGGGAGGTG GGTCCTTTGA GCCGGATACG GATCATGGCG ATGGCGGTGA TCCGGATGCC 
GGGGACCCGG CGCCCGATGA AGCAACGGAC GGCGATGCGC CCGCTGGTGG AGATGGTAGT 
GTTAACGGTA CTGCAGAAGG AGCTGCCGAT GCCGATGAGA GTAATGTAAA TAGTCCGGGT 
GAGGATGCAG CGGCGGCGGC AGCAGCAGCA GCAGCAGCGG CGGCGGCGGG CACGACGACG 
GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG CGACAGACCG CCGTTCTCGT GGAGAGCGAC 
GGGTTCGTGA CGAAGAACGG CCACAAGGTG GTCATCCACT CGCGATCGCC GAGCATCACG 
TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201696.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188625.1 Drosophila melanogaster paralytic (para), transcript variant AD, mRNA.

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CloneID	OFa03653
Clone ID Related Accession (Same CDS sequence)	NM_001201696.1
Accession Version	NM_001201696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG CAAAGGCGTT TGTCGTTGTA TATCTGCAGA GCATGGTGAC 
AACGAACTGG AGCTGGGCCA CGACGAGATC CTCGCCGACG GCCTCATCAA GAAGGGGATC 
AAGGAGCAGA CGCAACTGGA GGTGGCCATC GGGGATGGCA TGGAATTCAC GATACACGGC 
GACATGAAGA ACAACAAGCC GAAGAAATCC AAATATCTAA ATAACGCAAC GGACGACGAC 
ACTGCCAGCA TTAACTCATA TGGTAGCCAT AAGAATCGAC CATTCAAGGA CGAGAGCCAC 
AAGGGCAGCG CCGAGACGAT GGAGGGCGAG GAGAAGCGCG ACGCCAGCAA GGAGGATTTA 
GGTCTCGACG AGGAACTGGA CGAGGAGGGC GAATGCGAGG AGGGCCCGCT CGACGGTGAT 
ATCATTATTC ATGCACACGA CGAGGATATA CTCGATGAAT ATCCAGCTGA TTGCTGCCCC 
GATTCGTACT ATAAGAAATT TCCGATCTTA GCCGGTGACG ATGACTCGCC GTTCTGGCAA 
GGATGGGGCA ATTTACGACT GAAAACTTTT CAATTAATTG AAAATAAATA TTTTGAAACA 
GCTGTTATCA CTATGATTTT AATGAGTAGC TTAGCTTTGG CATTAGAAGA TGTACATCTG 
CCACAAAGAC CCATACTGCA GGATATTTTA TACTATATGG ACAGAATATT TACGGTTATA 
TTCTTCTTGG AAATGTTAAT CAAGTGGTTG GCGCTCGGCT TCAAAGTGTA CTTCACCAAC 
GCGTGGTGTT GGCTCGATTT CGTGATTGTC ATGCTGTCGC TAATTAATTT GGCCGCGGTC 
TGGTCCGGGG CCGATGATGT GCCCGCCTTT CGCTCGATGC GAACTCTGCG CGCCCTGCGA 
CCACTGCGGG CAGTTTCGCG CTGGGAGGGC ATGAAAGTCG TCGTTAATGC GCTGGTACAA 
GCTATACCGT CCATCTTCAA TGTGCTATTG GTGTGTCTAA TATTTTGGCT AATTTTTGCC 
ATAATGGGTG TACAGCTTTT TGCTGGAAAA TATTTTAAGT GCGAGGACAT GAATGGCACG 
AAGCTCAGCC ACGAGATCAT ACCAAATCGC AATGCCTGCG AGAGCGAGAA CTACACGTGG 
GTGAATTCAG CAATGAATTT CGATCATGTA GGTAACGCGT ATCTGTGCCT TTTCCAAGTG 
GCCACCTTCA AAGGCTGGAT ACAAATCATG AACGATGCTA TCGATTCACG AGAGGTGGAC 
AAGCAACCAA TTCGTGAAAC GAACATCTAC ATGTATTTAT ATTTCGTATT CTTCATCATA 
TTTGGATCAT TTTTCACACT CAATCTGTTC ATTGGTGTTA TCATTGATAA TTTTAATGAG 
CAAAAGAAAA AAGCAGGTGG ATCATTAGAA ATGTTCATGA CAGAAGATCA GAAAAAGTAC 
TATAATGCTA TGAAAAAGAT GGGCTCTAAA AAACCATTAA AAGCCATTCC AAGACCAAGG 
TGGCGACCAC AAGCAATAGT CTTTGAAATA GTAACCGATA AGAAATTCGA TATAATCATT 
ATGTTATTCA TTGGTCTGAA CATGTTCACC ATGACCCTCG ATCGTTACGA TGCGTCGGAC 
ACGTATAACG CGGTCCTAGA CTATCTCAAT GCGATATTCG TAGTTATTTT CAGTTCCGAA 
TGTCTATTAA AAATATTCGC TTTACGATAT CACTATTTTA TTGAGCCATG GAATTTATTT 
GATGTAGTAG TTGTCATTTT ATCCATCTTA GGTCTTGTAC TTAGCGATAT TATCGAGAAG 
TACTTCGTGT CGCCGACCCT GCTCCGAGTG GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT 
CGACTGGTGA AGGGAGCCAA GGGCATTCGG ACACTGCTCT TCGCGTTGGC CATGTCGCTG 
CCGGCCCTGT TCAACATCTG CCTGCTGCTG TTCCTGGTCA TGTTCATCTT TGCCATTTTC 
GGCATGTCGT TCTTCATGCA CGTGAAGGAG AAGAGCGGCA TCAACGACGT CTACAACTTC 
AAGACCTTTG GCCAGAGCAT GATCCTGCTC TTTCAGATGT CGACGTCAGC CGGTTGGGAT 
GGTGTACTGG ACGCCATTAT CAATGAGGAA GCATGCGATC CACCCGACAA CGACAAAGGC 
TATCCGGGCA ATTGTGGTTC AGCGACCGTT GGAATAACGT TTCTCCTCTC ATACCTAGTT 
ATAAGCTTTT TGATAGTTAT TAATATGTAC ATTGCTGTCA TTCTCGAGAA CTATAGTCAG 
GCCACCGAGG ACGTGCAAGA GGGTCTAACC GACGACGACT ACGACATGTA CTATGAGATC 
TGGCAGCAAT TCGATCCGGA GGGCACCCAG TACATACGCT ATGATCAGCT GTCCGAATTC 
CTGGACGTAC TGGAGCCCCC GCTGCAGATC CACAAACCGA ACAAGTACAA GATCATATCG 
ATGGACATAC CCATCTGTCG CGGTGACCTC ATGTACTGCG TCGACATCCT CGACGCCCTT 
ACGAAAGACT TCTTTGCGCG GAAGGGCAAT CCGATAGAGG AGACGGGTGA GATTGGTGAG 
ATAGCGGCCC GCCCGGATAC GGAGGGCTAC GAGCCCGTCT CATCAACGCT GTGGCGTCAG 
CGTGAGGAGT ACTGCGCCCG GCTAATCCAG CACGCCTGGC GAAAGCACAA GGCGCGCGGC 
GAGGGAGGTG GGTCCTTTGA GCCGGATACG GATCATGGCG ATGGCGGTGA TCCGGATGCC 
GGGGACCCGG CGCCCGATGA AGCAACGGAC GGCGATGCGC CCGCTGGTGG AGATGGTAGT 
GTTAACGGTA CTGCAGAAGG AGCTGCCGAT GCCGATGAGA GTAATGTAAA TAGTCCGGGT 
GAGGATGCAG CGGCGGCGGC AGCAGCAGCA GCAGCAGCGG CGGCGGCGGG CACGACGACG 
GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG CGACAGACCG CCGTTCTCGT GGAGAGCGAC 
GGGTTCGTGA CGAAGAACGG CCACAAGGTG GTCATCCACT CGCGATCGCC GAGCATCACG 
TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188625.1
CDS	269..6529
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201696.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AD, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201696.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG CAAAGGCGTT TGTCGTTGTA TATCTGCAGA GCATGGTGAC 
AACGAACTGG AGCTGGGCCA CGACGAGATC CTCGCCGACG GCCTCATCAA GAAGGGGATC 
AAGGAGCAGA CGCAACTGGA GGTGGCCATC GGGGATGGCA TGGAATTCAC GATACACGGC 
GACATGAAGA ACAACAAGCC GAAGAAATCC AAATATCTAA ATAACGCAAC GGACGACGAC 
ACTGCCAGCA TTAACTCATA TGGTAGCCAT AAGAATCGAC CATTCAAGGA CGAGAGCCAC 
AAGGGCAGCG CCGAGACGAT GGAGGGCGAG GAGAAGCGCG ACGCCAGCAA GGAGGATTTA 
GGTCTCGACG AGGAACTGGA CGAGGAGGGC GAATGCGAGG AGGGCCCGCT CGACGGTGAT 
ATCATTATTC ATGCACACGA CGAGGATATA CTCGATGAAT ATCCAGCTGA TTGCTGCCCC 
GATTCGTACT ATAAGAAATT TCCGATCTTA GCCGGTGACG ATGACTCGCC GTTCTGGCAA 
GGATGGGGCA ATTTACGACT GAAAACTTTT CAATTAATTG AAAATAAATA TTTTGAAACA 
GCTGTTATCA CTATGATTTT AATGAGTAGC TTAGCTTTGG CATTAGAAGA TGTACATCTG 
CCACAAAGAC CCATACTGCA GGATATTTTA TACTATATGG ACAGAATATT TACGGTTATA 
TTCTTCTTGG AAATGTTAAT CAAGTGGTTG GCGCTCGGCT TCAAAGTGTA CTTCACCAAC 
GCGTGGTGTT GGCTCGATTT CGTGATTGTC ATGCTGTCGC TAATTAATTT GGCCGCGGTC 
TGGTCCGGGG CCGATGATGT GCCCGCCTTT CGCTCGATGC GAACTCTGCG CGCCCTGCGA 
CCACTGCGGG CAGTTTCGCG CTGGGAGGGC ATGAAAGTCG TCGTTAATGC GCTGGTACAA 
GCTATACCGT CCATCTTCAA TGTGCTATTG GTGTGTCTAA TATTTTGGCT AATTTTTGCC 
ATAATGGGTG TACAGCTTTT TGCTGGAAAA TATTTTAAGT GCGAGGACAT GAATGGCACG 
AAGCTCAGCC ACGAGATCAT ACCAAATCGC AATGCCTGCG AGAGCGAGAA CTACACGTGG 
GTGAATTCAG CAATGAATTT CGATCATGTA GGTAACGCGT ATCTGTGCCT TTTCCAAGTG 
GCCACCTTCA AAGGCTGGAT ACAAATCATG AACGATGCTA TCGATTCACG AGAGGTGGAC 
AAGCAACCAA TTCGTGAAAC GAACATCTAC ATGTATTTAT ATTTCGTATT CTTCATCATA 
TTTGGATCAT TTTTCACACT CAATCTGTTC ATTGGTGTTA TCATTGATAA TTTTAATGAG 
CAAAAGAAAA AAGCAGGTGG ATCATTAGAA ATGTTCATGA CAGAAGATCA GAAAAAGTAC 
TATAATGCTA TGAAAAAGAT GGGCTCTAAA AAACCATTAA AAGCCATTCC AAGACCAAGG 
TGGCGACCAC AAGCAATAGT CTTTGAAATA GTAACCGATA AGAAATTCGA TATAATCATT 
ATGTTATTCA TTGGTCTGAA CATGTTCACC ATGACCCTCG ATCGTTACGA TGCGTCGGAC 
ACGTATAACG CGGTCCTAGA CTATCTCAAT GCGATATTCG TAGTTATTTT CAGTTCCGAA 
TGTCTATTAA AAATATTCGC TTTACGATAT CACTATTTTA TTGAGCCATG GAATTTATTT 
GATGTAGTAG TTGTCATTTT ATCCATCTTA GGTCTTGTAC TTAGCGATAT TATCGAGAAG 
TACTTCGTGT CGCCGACCCT GCTCCGAGTG GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT 
CGACTGGTGA AGGGAGCCAA GGGCATTCGG ACACTGCTCT TCGCGTTGGC CATGTCGCTG 
CCGGCCCTGT TCAACATCTG CCTGCTGCTG TTCCTGGTCA TGTTCATCTT TGCCATTTTC 
GGCATGTCGT TCTTCATGCA CGTGAAGGAG AAGAGCGGCA TCAACGACGT CTACAACTTC 
AAGACCTTTG GCCAGAGCAT GATCCTGCTC TTTCAGATGT CGACGTCAGC CGGTTGGGAT 
GGTGTACTGG ACGCCATTAT CAATGAGGAA GCATGCGATC CACCCGACAA CGACAAAGGC 
TATCCGGGCA ATTGTGGTTC AGCGACCGTT GGAATAACGT TTCTCCTCTC ATACCTAGTT 
ATAAGCTTTT TGATAGTTAT TAATATGTAC ATTGCTGTCA TTCTCGAGAA CTATAGTCAG 
GCCACCGAGG ACGTGCAAGA GGGTCTAACC GACGACGACT ACGACATGTA CTATGAGATC 
TGGCAGCAAT TCGATCCGGA GGGCACCCAG TACATACGCT ATGATCAGCT GTCCGAATTC 
CTGGACGTAC TGGAGCCCCC GCTGCAGATC CACAAACCGA ACAAGTACAA GATCATATCG 
ATGGACATAC CCATCTGTCG CGGTGACCTC ATGTACTGCG TCGACATCCT CGACGCCCTT 
ACGAAAGACT TCTTTGCGCG GAAGGGCAAT CCGATAGAGG AGACGGGTGA GATTGGTGAG 
ATAGCGGCCC GCCCGGATAC GGAGGGCTAC GAGCCCGTCT CATCAACGCT GTGGCGTCAG 
CGTGAGGAGT ACTGCGCCCG GCTAATCCAG CACGCCTGGC GAAAGCACAA GGCGCGCGGC 
GAGGGAGGTG GGTCCTTTGA GCCGGATACG GATCATGGCG ATGGCGGTGA TCCGGATGCC 
GGGGACCCGG CGCCCGATGA AGCAACGGAC GGCGATGCGC CCGCTGGTGG AGATGGTAGT 
GTTAACGGTA CTGCAGAAGG AGCTGCCGAT GCCGATGAGA GTAATGTAAA TAGTCCGGGT 
GAGGATGCAG CGGCGGCGGC AGCAGCAGCA GCAGCAGCGG CGGCGGCGGG CACGACGACG 
GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG CGACAGACCG CCGTTCTCGT GGAGAGCGAC 
GGGTTCGTGA CGAAGAACGG CCACAAGGTG GTCATCCACT CGCGATCGCC GAGCATCACG 
TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201704.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188633.1 Drosophila melanogaster paralytic (para), transcript variant AL, mRNA.

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CloneID	OFa03654
Clone ID Related Accession (Same CDS sequence)	NM_001201704.1
Accession Version	NM_001201704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AL
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG CAAAGGCGTT TGTCGTTGTA TATCTGCAGA GCATGGTGAC 
AACGAACTGG AGCTGGGCCA CGACGAGATC CTCGCCGACG GCCTCATCAA GAAGGGGATC 
AAGGAGCAGA CGCAACTGGA GGTGGCCATC GGGGATGGCA TGGAATTCAC GATACACGGC 
GACATGAAGA ACAACAAGCC GAAGAAATCC AAATATCTAA ATAACGCAAC GATGATTGGC 
AACTCAATTA ACCACCAAGA CAATAGACTG GAACACGAGC TAAACCATAG AGGTTTGTCC 
TTACAGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGGT ATCGCTTATC 
AACTTCGTTG CTTCACTTGT TGGAGCTGGT GGTATTCAAG CCTTCAAGAC TATGCGAACG 
TTAAGAGCAC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGCATGAG GGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188633.1
CDS	269..6604
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATMIGNSINHQDNRLEHELNHRGLSLQDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201704.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AL, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201704.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG CAAAGGCGTT TGTCGTTGTA TATCTGCAGA GCATGGTGAC 
AACGAACTGG AGCTGGGCCA CGACGAGATC CTCGCCGACG GCCTCATCAA GAAGGGGATC 
AAGGAGCAGA CGCAACTGGA GGTGGCCATC GGGGATGGCA TGGAATTCAC GATACACGGC 
GACATGAAGA ACAACAAGCC GAAGAAATCC AAATATCTAA ATAACGCAAC GATGATTGGC 
AACTCAATTA ACCACCAAGA CAATAGACTG GAACACGAGC TAAACCATAG AGGTTTGTCC 
TTACAGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGGT ATCGCTTATC 
AACTTCGTTG CTTCACTTGT TGGAGCTGGT GGTATTCAAG CCTTCAAGAC TATGCGAACG 
TTAAGAGCAC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGCATGAG GGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201718.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188647.1 Drosophila melanogaster paralytic (para), transcript variant AZ, mRNA.

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CloneID	OFa03655
Clone ID Related Accession (Same CDS sequence)	NM_001201718.1
Accession Version	NM_001201718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AZ
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG CAAAGGCGTT TGTCGTTGTA TATCTGCAGA GCATGGTGAC 
AACGAACTGG AGCTGGGCCA CGACGAGATC CTCGCCGACG GCCTCATCAA GAAGGGGATC 
AAGGAGCAGA CGCAACTGGA GGTGGCCATC GGGGATGGCA TGGAATTCAC GATACACGGC 
GACATGAAGA ACAACAAGCC GAAGAAATCC AAATATCTAA ATAACGCAAC GATGATTGGC 
AACTCAATTA ACCACCAAGA CAATAGACTG GAACACGAGC TAAACCATAG AGGTTTGTCC 
TTACAGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGCT GTCGCTAATT 
AATTTGGCCG CGGTCTGGTC CGGGGCCGAT GATGTGCCCG CCTTTCGCTC GATGCGAACT 
CTGCGCGCCC TGCGACCACT GCGGGCAGTT TCGCGCTGGG AGGGCATGAA AGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188647.1
CDS	269..6604
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATMIGNSINHQDNRLEHELNHRGLSLQDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201718.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AZ, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201718.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG CAAAGGCGTT TGTCGTTGTA TATCTGCAGA GCATGGTGAC 
AACGAACTGG AGCTGGGCCA CGACGAGATC CTCGCCGACG GCCTCATCAA GAAGGGGATC 
AAGGAGCAGA CGCAACTGGA GGTGGCCATC GGGGATGGCA TGGAATTCAC GATACACGGC 
GACATGAAGA ACAACAAGCC GAAGAAATCC AAATATCTAA ATAACGCAAC GATGATTGGC 
AACTCAATTA ACCACCAAGA CAATAGACTG GAACACGAGC TAAACCATAG AGGTTTGTCC 
TTACAGGACG ACGACACTGC CAGCATTAAC TCATATGGTA GCCATAAGAA TCGACCATTC 
AAGGACGAGA GCCACAAGGG CAGCGCCGAG ACGATGGAGG GCGAGGAGAA GCGCGACGCC 
AGCAAGGAGG ATTTAGGTCT CGACGAGGAA CTGGACGAGG AGGGCGAATG CGAGGAGGGC 
CCGCTCGACG GTGATATCAT TATTCATGCA CACGACGAGG ATATACTCGA TGAATATCCA 
GCTGATTGCT GCCCCGATTC GTACTATAAG AAATTTCCGA TCTTAGCCGG TGACGATGAC 
TCGCCGTTCT GGCAAGGATG GGGCAATTTA CGACTGAAAA CTTTTCAATT AATTGAAAAT 
AAATATTTTG AAACAGCTGT TATCACTATG ATTTTAATGA GTAGCTTAGC TTTGGCATTA 
GAAGATGTAC ATCTGCCACA AAGACCCATA CTGCAGGATA TTTTATACTA TATGGACAGA 
ATATTTACGG TTATATTCTT CTTGGAAATG TTAATCAAGT GGTTGGCGCT CGGCTTCAAA 
GTGTACTTCA CCAACGCGTG GTGTTGGCTC GATTTCGTGA TTGTCATGCT GTCGCTAATT 
AATTTGGCCG CGGTCTGGTC CGGGGCCGAT GATGTGCCCG CCTTTCGCTC GATGCGAACT 
CTGCGCGCCC TGCGACCACT GCGGGCAGTT TCGCGCTGGG AGGGCATGAA AGTCGTCGTT 
AATGCGCTGG TACAAGCTAT ACCGTCCATC TTCAATGTGC TATTGGTGTG TCTAATATTT 
TGGCTAATTT TTGCCATAAT GGGTGTACAG CTTTTTGCTG GAAAATATTT TAAGTGCGAG 
GACATGAATG GCACGAAGCT CAGCCACGAG ATCATACCAA ATCGCAATGC CTGCGAGAGC 
GAGAACTACA CGTGGGTGAA TTCAGCAATG AATTTCGATC ATGTAGGTAA CGCGTATCTG 
TGCCTTTTCC AAGTGGCCAC CTTCAAAGGC TGGATACAAA TCATGAACGA TGCTATCGAT 
TCACGAGAGG TGGACAAGCA ACCAATTCGT GAAACGAACA TCTACATGTA TTTATATTTC 
GTATTCTTCA TCATATTTGG ATCATTTTTC ACACTCAATC TGTTCATTGG TGTTATCATT 
GATAATTTTA ATGAGCAAAA GAAAAAAGCA GGTGGATCAT TAGAAATGTT CATGACAGAA 
GATCAGAAAA AGTACTATAA TGCTATGAAA AAGATGGGCT CTAAAAAACC ATTAAAAGCC 
ATTCCAAGAC CAAGGTGGCG ACCACAAGCA ATAGTCTTTG AAATAGTAAC CGATAAGAAA 
TTCGATATAA TCATTATGTT ATTCATTGGT CTGAACATGT TCACCATGAC CCTCGATCGT 
TACGATGCGT CGGACACGTA TAACGCGGTC CTAGACTATC TCAATGCGAT ATTCGTAGTT 
ATTTTCAGTT CCGAATGTCT ATTAAAAATA TTCGCTTTAC GATATCACTA TTTTATTGAG 
CCATGGAATT TATTTGATGT AGTAGTTGTC ATTTTATCCA TCTTAGGTCT TGTACTTAGC 
GATATTATCG AGAAGTACTT CGTGTCGCCG ACCCTGCTCC GAGTGGTGCG TGTGGCGAAA 
GTGGGCCGTG TCCTTCGACT GGTGAAGGGA GCCAAGGGCA TTCGGACACT GCTCTTCGCG 
TTGGCCATGT CGCTGCCGGC CCTGTTCAAC ATCTGCCTGC TGCTGTTCCT GGTCATGTTC 
ATCTTTGCCA TTTTCGGCAT GTCGTTCTTC ATGCACGTGA AGGAGAAGAG CGGCATCAAC 
GACGTCTACA ACTTCAAGAC CTTTGGCCAG AGCATGATCC TGCTCTTTCA GATGTCGACG 
TCAGCCGGTT GGGATGGTGT ACTGGACGCC ATTATCAATG AGGAAGCATG CGATCCACCC 
GACAACGACA AAGGCTATCC GGGCAATTGT GGTTCAGCGA CCGTTGGAAT AACGTTTCTC 
CTCTCATACC TAGTTATAAG CTTTTTGATA GTTATTAATA TGTACATTGC TGTCATTCTC 
GAGAACTATA GTCAGGCCAC CGAGGACGTG CAAGAGGGTC TAACCGACGA CGACTACGAC 
ATGTACTATG AGATCTGGCA GCAATTCGAT CCGGAGGGCA CCCAGTACAT ACGCTATGAT 
CAGCTGTCCG AATTCCTGGA CGTACTGGAG CCCCCGCTGC AGATCCACAA ACCGAACAAG 
TACAAGATCA TATCGATGGA CATACCCATC TGTCGCGGTG ACCTCATGTA CTGCGTCGAC 
ATCCTCGACG CCCTTACGAA AGACTTCTTT GCGCGGAAGG GCAATCCGAT AGAGGAGACG 
GGTGAGATTG GTGAGATAGC GGCCCGCCCG GATACGGAGG GCTACGAGCC CGTCTCATCA 
ACGCTGTGGC GTCAGCGTGA GGAGTACTGC GCCCGGCTAA TCCAGCACGC CTGGCGAAAG 
CACAAGGCGC GCGGCGAGGG AGGTGGGTCC TTTGAGCCGG ATACGGATCA TGGCGATGGC 
GGTGATCCGG ATGCCGGGGA CCCGGCGCCC GATGAAGCAA CGGACGGCGA TGCGCCCGCT 
GGTGGAGATG GTAGTGTTAA CGGTACTGCA GAAGGAGCTG CCGATGCCGA TGAGAGTAAT 
GTAAATAGTC CGGGTGAGGA TGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCGGCGGCG 
GCGGGCACGA CGACGGCGGG AAGTCCCGGA GCGGGTAGCG CCGGGCGACA GACCGCCGTT 
CTCGTGGAGA GCGACGGGTT CGTGACGAAG AACGGCCACA AGGTGGTCAT CCACTCGCGA 
TCGCCGAGCA TCACGTCGCG CACGGCGGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201699.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188628.1 Drosophila melanogaster paralytic (para), transcript variant AG, mRNA.

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CloneID	OFa03656
Clone ID Related Accession (Same CDS sequence)	NM_001201699.1
Accession Version	NM_001201699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AG
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG TGAGAGGACC AACCAGATCA GTTGGATTTG GAGCGAAGAG 
CATGGTGACA ACGAACTGGA GCTGGGCCAC GACGAGATCC TCGCCGACGG CCTCATCAAG 
AAGGGGATCA AGGAGCAGAC GCAACTGGAG GTGGCCATCG GGGATGGCAT GGAATTCACG 
ATACACGGCG ACATGAAGAA CAACAAGCCG AAGAAATCCA AATATCTAAA TAACGCAACG 
GACGACGACA CTGCCAGCAT TAACTCATAT GGTAGCCATA AGAATCGACC ATTCAAGGAC 
GAGAGCCACA AGGGCAGCGC CGAGACGATG GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG 
GAGGATTTAG GTCTCGACGA GGAACTGGAC GAGGAGGGCG AATGCGAGGA GGGCCCGCTC 
GACGGTGATA TCATTATTCA TGCACACGAC GAGGATATAC TCGATGAATA TCCAGCTGAT 
TGCTGCCCCG ATTCGTACTA TAAGAAATTT CCGATCTTAG CCGGTGACGA TGACTCGCCG 
TTCTGGCAAG GATGGGGCAA TTTACGACTG AAAACTTTTC AATTAATTGA AAATAAATAT 
TTTGAAACAG CTGTTATCAC TATGATTTTA ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT 
GTACATCTGC CACAAAGACC CATACTGCAG GATATTTTAT ACTATATGGA CAGAATATTT 
ACGGTTATAT TCTTCTTGGA AATGTTAATC AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC 
TTCACCAACG CGTGGTGTTG GCTCGATTTC GTGATTGTCA TGGTATCGCT TATCAACTTC 
GTTGCTTCAC TTGTTGGAGC TGGTGGTATT CAAGCCTTCA AGACTATGCG AACGTTAAGA 
GCACTGAGAC CACTACGTGC CATGTCCCGT ATGCAGGGCA TGAGGGTCGT CGTTAATGCG 
CTGGTACAAG CTATACCGTC CATCTTCAAT GTGCTATTGG TGTGTCTAAT ATTTTGGCTA 
ATTTTTGCCA TAATGGGTGT ACAGCTTTTT GCTGGAAAAT ATTTTAAGTG CGAGGACATG 
AATGGCACGA AGCTCAGCCA CGAGATCATA CCAAATCGCA ATGCCTGCGA GAGCGAGAAC 
TACACGTGGG TGAATTCAGC AATGAATTTC GATCATGTAG GTAACGCGTA TCTGTGCCTT 
TTCCAAGTGG CCACCTTCAA AGGCTGGATA CAAATCATGA ACGATGCTAT CGATTCACGA 
GAGGTGGACA AGCAACCAAT TCGTGAAACG AACATCTACA TGTATTTATA TTTCGTATTC 
TTCATCATAT TTGGATCATT TTTCACACTC AATCTGTTCA TTGGTGTTAT CATTGATAAT 
TTTAATGAGC AAAAGAAAAA AGCAGGTGGA TCATTAGAAA TGTTCATGAC AGAAGATCAG 
AAAAAGTACT ATAATGCTAT GAAAAAGATG GGCTCTAAAA AACCATTAAA AGCCATTCCA 
AGACCAAGGT GGCGACCACA AGCAATAGTC TTTGAAATAG TAACCGATAA GAAATTCGAT 
ATAATCATTA TGTTATTCAT TGGTCTGAAC ATGTTCACCA TGACCCTCGA TCGTTACGAT 
GCGTCGGACA CGTATAACGC GGTCCTAGAC TATCTCAATG CGATATTCGT AGTTATTTTC 
AGTTCCGAAT GTCTATTAAA AATATTCGCT TTACGATATC ACTATTTTAT TGAGCCATGG 
AATTTATTTG ATGTAGTAGT TGTCATTTTA TCCATCTTAG GTCTTGTACT TAGCGATATT 
ATCGAGAAGT ACTTCGTGTC GCCGACCCTG CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC 
CGTGTCCTTC GACTGGTGAA GGGAGCCAAG GGCATTCGGA CACTGCTCTT CGCGTTGGCC 
ATGTCGCTGC CGGCCCTGTT CAACATCTGC CTGCTGCTGT TCCTGGTCAT GTTCATCTTT 
GCCATTTTCG GCATGTCGTT CTTCATGCAC GTGAAGGAGA AGAGCGGCAT CAACGACGTC 
TACAACTTCA AGACCTTTGG CCAGAGCATG ATCCTGCTCT TTCAGATGTC GACGTCAGCC 
GGTTGGGATG GTGTACTGGA CGCCATTATC AATGAGGAAG CATGCGATCC ACCCGACAAC 
GACAAAGGCT ATCCGGGCAA TTGTGGTTCA GCGACCGTTG GAATAACGTT TCTCCTCTCA 
TACCTAGTTA TAAGCTTTTT GATAGTTATT AATATGTACA TTGCTGTCAT TCTCGAGAAC 
TATAGTCAGG CCACCGAGGA CGTGCAAGAG GGTCTAACCG ACGACGACTA CGACATGTAC 
TATGAGATCT GGCAGCAATT CGATCCGGAG GGCACCCAGT ACATACGCTA TGATCAGCTG 
TCCGAATTCC TGGACGTACT GGAGCCCCCG CTGCAGATCC ACAAACCGAA CAAGTACAAG 
ATCATATCGA TGGACATACC CATCTGTCGC GGTGACCTCA TGTACTGCGT CGACATCCTC 
GACGCCCTTA CGAAAGACTT CTTTGCGCGG AAGGGCAATC CGATAGAGGA GACGGGTGAG 
ATTGGTGAGA TAGCGGCCCG CCCGGATACG GAGGGCTACG AGCCCGTCTC ATCAACGCTG 
TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG CTAATCCAGC ACGCCTGGCG AAAGCACAAG 
GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG CCGGATACGG ATCATGGCGA TGGCGGTGAT 
CCGGATGCCG GGGACCCGGC GCCCGATGAA GCAACGGACG GCGATGCGCC CGCTGGTGGA 
GATGGTAGTG TTAACGGTAC TGCAGAAGGA GCTGCCGATG CCGATGAGAG TAATGTAAAT 
AGTCCGGGTG AGGATGCAGC GGCGGCGGCA GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC 
ACGACGACGG CGGGAAGTCC CGGAGCGGGT AGCGCCGGGC GACAGACCGC CGTTCTCGTG 
GAGAGCGACG GGTTCGTGAC GAAGAACGGC CACAAGGTGG TCATCCACTC GCGATCGCCG 
AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188628.1
CDS	269..6538
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201699.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AG, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201699.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG TGAGAGGACC AACCAGATCA GTTGGATTTG GAGCGAAGAG 
CATGGTGACA ACGAACTGGA GCTGGGCCAC GACGAGATCC TCGCCGACGG CCTCATCAAG 
AAGGGGATCA AGGAGCAGAC GCAACTGGAG GTGGCCATCG GGGATGGCAT GGAATTCACG 
ATACACGGCG ACATGAAGAA CAACAAGCCG AAGAAATCCA AATATCTAAA TAACGCAACG 
GACGACGACA CTGCCAGCAT TAACTCATAT GGTAGCCATA AGAATCGACC ATTCAAGGAC 
GAGAGCCACA AGGGCAGCGC CGAGACGATG GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG 
GAGGATTTAG GTCTCGACGA GGAACTGGAC GAGGAGGGCG AATGCGAGGA GGGCCCGCTC 
GACGGTGATA TCATTATTCA TGCACACGAC GAGGATATAC TCGATGAATA TCCAGCTGAT 
TGCTGCCCCG ATTCGTACTA TAAGAAATTT CCGATCTTAG CCGGTGACGA TGACTCGCCG 
TTCTGGCAAG GATGGGGCAA TTTACGACTG AAAACTTTTC AATTAATTGA AAATAAATAT 
TTTGAAACAG CTGTTATCAC TATGATTTTA ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT 
GTACATCTGC CACAAAGACC CATACTGCAG GATATTTTAT ACTATATGGA CAGAATATTT 
ACGGTTATAT TCTTCTTGGA AATGTTAATC AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC 
TTCACCAACG CGTGGTGTTG GCTCGATTTC GTGATTGTCA TGGTATCGCT TATCAACTTC 
GTTGCTTCAC TTGTTGGAGC TGGTGGTATT CAAGCCTTCA AGACTATGCG AACGTTAAGA 
GCACTGAGAC CACTACGTGC CATGTCCCGT ATGCAGGGCA TGAGGGTCGT CGTTAATGCG 
CTGGTACAAG CTATACCGTC CATCTTCAAT GTGCTATTGG TGTGTCTAAT ATTTTGGCTA 
ATTTTTGCCA TAATGGGTGT ACAGCTTTTT GCTGGAAAAT ATTTTAAGTG CGAGGACATG 
AATGGCACGA AGCTCAGCCA CGAGATCATA CCAAATCGCA ATGCCTGCGA GAGCGAGAAC 
TACACGTGGG TGAATTCAGC AATGAATTTC GATCATGTAG GTAACGCGTA TCTGTGCCTT 
TTCCAAGTGG CCACCTTCAA AGGCTGGATA CAAATCATGA ACGATGCTAT CGATTCACGA 
GAGGTGGACA AGCAACCAAT TCGTGAAACG AACATCTACA TGTATTTATA TTTCGTATTC 
TTCATCATAT TTGGATCATT TTTCACACTC AATCTGTTCA TTGGTGTTAT CATTGATAAT 
TTTAATGAGC AAAAGAAAAA AGCAGGTGGA TCATTAGAAA TGTTCATGAC AGAAGATCAG 
AAAAAGTACT ATAATGCTAT GAAAAAGATG GGCTCTAAAA AACCATTAAA AGCCATTCCA 
AGACCAAGGT GGCGACCACA AGCAATAGTC TTTGAAATAG TAACCGATAA GAAATTCGAT 
ATAATCATTA TGTTATTCAT TGGTCTGAAC ATGTTCACCA TGACCCTCGA TCGTTACGAT 
GCGTCGGACA CGTATAACGC GGTCCTAGAC TATCTCAATG CGATATTCGT AGTTATTTTC 
AGTTCCGAAT GTCTATTAAA AATATTCGCT TTACGATATC ACTATTTTAT TGAGCCATGG 
AATTTATTTG ATGTAGTAGT TGTCATTTTA TCCATCTTAG GTCTTGTACT TAGCGATATT 
ATCGAGAAGT ACTTCGTGTC GCCGACCCTG CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC 
CGTGTCCTTC GACTGGTGAA GGGAGCCAAG GGCATTCGGA CACTGCTCTT CGCGTTGGCC 
ATGTCGCTGC CGGCCCTGTT CAACATCTGC CTGCTGCTGT TCCTGGTCAT GTTCATCTTT 
GCCATTTTCG GCATGTCGTT CTTCATGCAC GTGAAGGAGA AGAGCGGCAT CAACGACGTC 
TACAACTTCA AGACCTTTGG CCAGAGCATG ATCCTGCTCT TTCAGATGTC GACGTCAGCC 
GGTTGGGATG GTGTACTGGA CGCCATTATC AATGAGGAAG CATGCGATCC ACCCGACAAC 
GACAAAGGCT ATCCGGGCAA TTGTGGTTCA GCGACCGTTG GAATAACGTT TCTCCTCTCA 
TACCTAGTTA TAAGCTTTTT GATAGTTATT AATATGTACA TTGCTGTCAT TCTCGAGAAC 
TATAGTCAGG CCACCGAGGA CGTGCAAGAG GGTCTAACCG ACGACGACTA CGACATGTAC 
TATGAGATCT GGCAGCAATT CGATCCGGAG GGCACCCAGT ACATACGCTA TGATCAGCTG 
TCCGAATTCC TGGACGTACT GGAGCCCCCG CTGCAGATCC ACAAACCGAA CAAGTACAAG 
ATCATATCGA TGGACATACC CATCTGTCGC GGTGACCTCA TGTACTGCGT CGACATCCTC 
GACGCCCTTA CGAAAGACTT CTTTGCGCGG AAGGGCAATC CGATAGAGGA GACGGGTGAG 
ATTGGTGAGA TAGCGGCCCG CCCGGATACG GAGGGCTACG AGCCCGTCTC ATCAACGCTG 
TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG CTAATCCAGC ACGCCTGGCG AAAGCACAAG 
GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG CCGGATACGG ATCATGGCGA TGGCGGTGAT 
CCGGATGCCG GGGACCCGGC GCCCGATGAA GCAACGGACG GCGATGCGCC CGCTGGTGGA 
GATGGTAGTG TTAACGGTAC TGCAGAAGGA GCTGCCGATG CCGATGAGAG TAATGTAAAT 
AGTCCGGGTG AGGATGCAGC GGCGGCGGCA GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC 
ACGACGACGG CGGGAAGTCC CGGAGCGGGT AGCGCCGGGC GACAGACCGC CGTTCTCGTG 
GAGAGCGACG GGTTCGTGAC GAAGAACGGC CACAAGGTGG TCATCCACTC GCGATCGCCG 
AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201716.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188645.1 Drosophila melanogaster paralytic (para), transcript variant AX, mRNA.

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CloneID	OFa03658
Clone ID Related Accession (Same CDS sequence)	NM_001201716.1
Accession Version	NM_001201716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AX
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGAGGACG ATGACGAGGA TGGGCCGACG 
TTCAAAGACA AGGCACTCGA AGTGATCCTC AAAGGCATCG ATGTGTTTTG TGTGTGGGAC 
TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG TGGGTATCGC TCATCGTCTT CGATCCCTTC 
GTCGAGCTCT TCATCACGCT GTGCATTGTG GTCAACACGA TGTTCATGGC AATGGATCAC 
CACGATATGA ACAAGGAGAT GGAACGCGTG CTCAAGAGTG GCAACTATTT CTTCACCGCC 
ACCTTTGCCA TCGAGGCCAC CATGAAGCTA ATGGCCATGA GCCCCAAGTA CTATTTCCAG 
GAGGGCTGGA ACATCTTCGA CTTCATTATC GTGGCCCTAT CGCTATTGGA ACTGGGACTC 
GAGGGTGTCC AGGGTCTGTC CGTATTGCGT TCCTTTCGAT TGCTGCGTGT ATTCAAACTG 
GCCAAGTCTT GGCCCACACT TAATTTACTC ATTTCGATTA TGGGACGCAC CATGGGCGCT 
TTGGGTAATC TGACATTTGT ACTTTGCATT ATCATCTTCA TCTTTGCGGT GATGGGAATG 
CAACTGTTCG GAAAGAATTA TCATGATCAC AAGGACCGCT TTCCGGATGG CGACCTGCCG 
CGCTGGAACT TCACCGACTT TATGCACAGC TTCATGATCG TGTTCCGGGT GCTCTGCGGA 
GAATGGATCG AGTCCATGTG GGACTGCATG TACGTGGGCG ATGTCTCGTG CATTCCCTTC 
TTCTTGGCCA CCGTTGTCAT CGGCAATCTT GTGGTACTTA ACCTTTTCTT AGCCTTGCTT 
TTGTCCAATT TTGGCTCATC TAGCTTATCA GCGCCGACTG CCGATAACGA TACGAATAAA 
ATAGCCGAGG CCTTCAATCG AATTGGCCGA TTTAAAAGTT GGGTTAAGCG TAATATTGCT 
GATTGTTTCA AGTTAATACG TAACAAATTG ACAAATCAAA TAAGTGATCA ACCATCAGGC 
AAAGGCGTTT GTCGTTGTAT ATCTGCAGAG CATGGTGACA ACGAACTGGA GCTGGGCCAC 
GACGAGATCC TCGCCGACGG CCTCATCAAG AAGGGGATCA AGGAGCAGAC GCAACTGGAG 
GTGGCCATCG GGGATGGCAT GGAATTCACG ATACACGGCG ACATGAAGAA CAACAAGCCG 
AAGAAATCCA AATATCTAAA TAACGCAACG GACGACGACA CTGCCAGCAT TAACTCATAT 
GGTAGCCATA AGAATCGACC ATTCAAGGAC GAGAGCCACA AGGGCAGCGC CGAGACGATG 
GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG GAGGATTTAG GTCTCGACGA GGAACTGGAC 
GAGGAGGGCG AATGCGAGGA GGGCCCGCTC GACGGTGATA TCATTATTCA TGCACACGAC 
GAGGATATAC TCGATGAATA TCCAGCTGAT TGCTGCCCCG ATTCGTACTA TAAGAAATTT 
CCGATCTTAG CCGGTGACGA TGACTCGCCG TTCTGGCAAG GATGGGGCAA TTTACGACTG 
AAAACTTTTC AATTAATTGA AAATAAATAT TTTGAAACAG CTGTTATCAC TATGATTTTA 
ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT GTACATCTGC CACAAAGACC CATACTGCAG 
GATATTTTAT ACTATATGGA CAGAATATTT ACGGTTATAT TCTTCTTGGA AATGTTAATC 
AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC TTCACCAACG CGTGGTGTTG GCTCGATTTC 
GTGATTGTCA TGGTATCGCT TATCAACTTC GTTGCTTCAC TTGTTGGAGC TGGTGGTATT 
CAAGCCTTCA AGACTATGCG AACGTTAAGA GCACTGAGAC CACTACGTGC CATGTCCCGT 
ATGCAGGGCA TGAGGGTCGT CGTTAATGCG CTGGTACAAG CTATACCGTC CATCTTCAAT 
GTGCTATTGG TGTGTCTAAT ATTTTGGCTA ATTTTTGCCA TAATGGGTGT ACAGCTTTTT 
GCTGGAAAAT ATTTTAAGTG CGAGGACATG AATGGCACGA AGCTCAGCCA CGAGATCATA 
CCAAATCGCA ATGCCTGCGA GAGCGAGAAC TACACGTGGG TGAATTCAGC AATGAATTTC 
GATCATGTAG GTAACGCGTA TCTGTGCCTT TTCCAAGTGG CCACCTTCAA AGGCTGGATA 
CAAATCATGA ACGATGCTAT CGATTCACGA GAGGTGGACA AGCAACCAAT TCGTGAAACG 
AACATCTACA TGTATTTATA TTTCGTATTC TTCATCATAT TTGGATCATT TTTCACACTC 
AATCTGTTCA TTGGTGTTAT CATTGATAAT TTTAATGAGC AAAAGAAAAA AGCAGGTGGA 
TCATTAGAAA TGTTCATGAC AGAAGATCAG AAAAAGTACT ATAATGCTAT GAAAAAGATG 
GGCTCTAAAA AACCATTAAA AGCCATTCCA AGACCAAGGT GGCGACCACA AGCAATAGTC 
TTTGAAATAG TAACCGATAA GAAATTCGAT ATAATCATTA TGTTATTCAT TGGTCTGAAC 
ATGTTCACCA TGACCCTCGA TCGTTACGAT GCGTCGGACA CGTATAACGC GGTCCTAGAC 
TATCTCAATG CGATATTCGT AGTTATTTTC AGTTCCGAAT GTCTATTAAA AATATTCGCT 
TTACGATATC ACTATTTTAT TGAGCCATGG AATTTATTTG ATGTAGTAGT TGTCATTTTA 
TCCATCTTAG GTCTTGTACT TAGCGATATT ATCGAGAAGT ACTTCGTGTC GCCGACCCTG 
CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC CGTGTCCTTC GACTGGTGAA GGGAGCCAAG 
GGCATTCGGA CACTGCTCTT CGCGTTGGCC ATGTCGCTGC CGGCCCTGTT CAACATCTGC 
CTGCTGCTGT TCCTGGTCAT GTTCATCTTT GCCATTTTCG GCATGTCGTT CTTCATGCAC 
GTGAAGGAGA AGAGCGGCAT CAACGACGTC TACAACTTCA AGACCTTTGG CCAGAGCATG 
ATCCTGCTCT TTCAGATGTC GACGTCAGCC GGTTGGGATG GTGTACTGGA CGCCATTATC 
AATGAGGAAG CATGCGATCC ACCCGACAAC GACAAAGGCT ATCCGGGCAA TTGTGGTTCA 
GCGACCGTTG GAATAACGTT TCTCCTCTCA TACCTAGTTA TAAGCTTTTT GATAGTTATT 
AATATGTACA TTGCTGTCAT TCTCGAGAAC TATAGTCAGG CCACCGAGGA CGTGCAAGAG 
GGTCTAACCG ACGACGACTA CGACATGTAC TATGAGATCT GGCAGCAATT CGATCCGGAG 
GGCACCCAGT ACATACGCTA TGATCAGCTG TCCGAATTCC TGGACGTACT GGAGCCCCCG 
CTGCAGATCC ACAAACCGAA CAAGTACAAG ATCATATCGA TGGACATACC CATCTGTCGC 
GGTGACCTCA TGTACTGCGT CGACATCCTC GACGCCCTTA CGAAAGACTT CTTTGCGCGG 
AAGGGCAATC CGATAGAGGA GACGGGTGAG ATTGGTGAGA TAGCGGCCCG CCCGGATACG 
GAGGGCTACG AGCCCGTCTC ATCAACGCTG TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG 
CTAATCCAGC ACGCCTGGCG AAAGCACAAG GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG 
CCGGATACGG ATCATGGCGA TGGCGGTGAT CCGGATGCCG GGGACCCGGC GCCCGATGAA 
GCAACGGACG GCGATGCGCC CGCTGGTGGA GATGGTAGTG TTAACGGTAC TGCAGAAGGA 
GCTGCCGATG CCGATGAGAG TAATGTAAAT AGTCCGGGTG AGGATGCAGC GGCGGCGGCA 
GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC ACGACGACGG CGGGAAGTCC CGGAGCGGGT 
AGCGCCGGGC GACAGACCGC CGTTCTCGTG GAGAGCGACG GGTTCGTGAC GAAGAACGGC 
CACAAGGTGG TCATCCACTC GCGATCGCCG AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188645.1
CDS	269..6568
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201716.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AX, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201716.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGAGGACG ATGACGAGGA TGGGCCGACG 
TTCAAAGACA AGGCACTCGA AGTGATCCTC AAAGGCATCG ATGTGTTTTG TGTGTGGGAC 
TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG TGGGTATCGC TCATCGTCTT CGATCCCTTC 
GTCGAGCTCT TCATCACGCT GTGCATTGTG GTCAACACGA TGTTCATGGC AATGGATCAC 
CACGATATGA ACAAGGAGAT GGAACGCGTG CTCAAGAGTG GCAACTATTT CTTCACCGCC 
ACCTTTGCCA TCGAGGCCAC CATGAAGCTA ATGGCCATGA GCCCCAAGTA CTATTTCCAG 
GAGGGCTGGA ACATCTTCGA CTTCATTATC GTGGCCCTAT CGCTATTGGA ACTGGGACTC 
GAGGGTGTCC AGGGTCTGTC CGTATTGCGT TCCTTTCGAT TGCTGCGTGT ATTCAAACTG 
GCCAAGTCTT GGCCCACACT TAATTTACTC ATTTCGATTA TGGGACGCAC CATGGGCGCT 
TTGGGTAATC TGACATTTGT ACTTTGCATT ATCATCTTCA TCTTTGCGGT GATGGGAATG 
CAACTGTTCG GAAAGAATTA TCATGATCAC AAGGACCGCT TTCCGGATGG CGACCTGCCG 
CGCTGGAACT TCACCGACTT TATGCACAGC TTCATGATCG TGTTCCGGGT GCTCTGCGGA 
GAATGGATCG AGTCCATGTG GGACTGCATG TACGTGGGCG ATGTCTCGTG CATTCCCTTC 
TTCTTGGCCA CCGTTGTCAT CGGCAATCTT GTGGTACTTA ACCTTTTCTT AGCCTTGCTT 
TTGTCCAATT TTGGCTCATC TAGCTTATCA GCGCCGACTG CCGATAACGA TACGAATAAA 
ATAGCCGAGG CCTTCAATCG AATTGGCCGA TTTAAAAGTT GGGTTAAGCG TAATATTGCT 
GATTGTTTCA AGTTAATACG TAACAAATTG ACAAATCAAA TAAGTGATCA ACCATCAGGC 
AAAGGCGTTT GTCGTTGTAT ATCTGCAGAG CATGGTGACA ACGAACTGGA GCTGGGCCAC 
GACGAGATCC TCGCCGACGG CCTCATCAAG AAGGGGATCA AGGAGCAGAC GCAACTGGAG 
GTGGCCATCG GGGATGGCAT GGAATTCACG ATACACGGCG ACATGAAGAA CAACAAGCCG 
AAGAAATCCA AATATCTAAA TAACGCAACG GACGACGACA CTGCCAGCAT TAACTCATAT 
GGTAGCCATA AGAATCGACC ATTCAAGGAC GAGAGCCACA AGGGCAGCGC CGAGACGATG 
GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG GAGGATTTAG GTCTCGACGA GGAACTGGAC 
GAGGAGGGCG AATGCGAGGA GGGCCCGCTC GACGGTGATA TCATTATTCA TGCACACGAC 
GAGGATATAC TCGATGAATA TCCAGCTGAT TGCTGCCCCG ATTCGTACTA TAAGAAATTT 
CCGATCTTAG CCGGTGACGA TGACTCGCCG TTCTGGCAAG GATGGGGCAA TTTACGACTG 
AAAACTTTTC AATTAATTGA AAATAAATAT TTTGAAACAG CTGTTATCAC TATGATTTTA 
ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT GTACATCTGC CACAAAGACC CATACTGCAG 
GATATTTTAT ACTATATGGA CAGAATATTT ACGGTTATAT TCTTCTTGGA AATGTTAATC 
AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC TTCACCAACG CGTGGTGTTG GCTCGATTTC 
GTGATTGTCA TGGTATCGCT TATCAACTTC GTTGCTTCAC TTGTTGGAGC TGGTGGTATT 
CAAGCCTTCA AGACTATGCG AACGTTAAGA GCACTGAGAC CACTACGTGC CATGTCCCGT 
ATGCAGGGCA TGAGGGTCGT CGTTAATGCG CTGGTACAAG CTATACCGTC CATCTTCAAT 
GTGCTATTGG TGTGTCTAAT ATTTTGGCTA ATTTTTGCCA TAATGGGTGT ACAGCTTTTT 
GCTGGAAAAT ATTTTAAGTG CGAGGACATG AATGGCACGA AGCTCAGCCA CGAGATCATA 
CCAAATCGCA ATGCCTGCGA GAGCGAGAAC TACACGTGGG TGAATTCAGC AATGAATTTC 
GATCATGTAG GTAACGCGTA TCTGTGCCTT TTCCAAGTGG CCACCTTCAA AGGCTGGATA 
CAAATCATGA ACGATGCTAT CGATTCACGA GAGGTGGACA AGCAACCAAT TCGTGAAACG 
AACATCTACA TGTATTTATA TTTCGTATTC TTCATCATAT TTGGATCATT TTTCACACTC 
AATCTGTTCA TTGGTGTTAT CATTGATAAT TTTAATGAGC AAAAGAAAAA AGCAGGTGGA 
TCATTAGAAA TGTTCATGAC AGAAGATCAG AAAAAGTACT ATAATGCTAT GAAAAAGATG 
GGCTCTAAAA AACCATTAAA AGCCATTCCA AGACCAAGGT GGCGACCACA AGCAATAGTC 
TTTGAAATAG TAACCGATAA GAAATTCGAT ATAATCATTA TGTTATTCAT TGGTCTGAAC 
ATGTTCACCA TGACCCTCGA TCGTTACGAT GCGTCGGACA CGTATAACGC GGTCCTAGAC 
TATCTCAATG CGATATTCGT AGTTATTTTC AGTTCCGAAT GTCTATTAAA AATATTCGCT 
TTACGATATC ACTATTTTAT TGAGCCATGG AATTTATTTG ATGTAGTAGT TGTCATTTTA 
TCCATCTTAG GTCTTGTACT TAGCGATATT ATCGAGAAGT ACTTCGTGTC GCCGACCCTG 
CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC CGTGTCCTTC GACTGGTGAA GGGAGCCAAG 
GGCATTCGGA CACTGCTCTT CGCGTTGGCC ATGTCGCTGC CGGCCCTGTT CAACATCTGC 
CTGCTGCTGT TCCTGGTCAT GTTCATCTTT GCCATTTTCG GCATGTCGTT CTTCATGCAC 
GTGAAGGAGA AGAGCGGCAT CAACGACGTC TACAACTTCA AGACCTTTGG CCAGAGCATG 
ATCCTGCTCT TTCAGATGTC GACGTCAGCC GGTTGGGATG GTGTACTGGA CGCCATTATC 
AATGAGGAAG CATGCGATCC ACCCGACAAC GACAAAGGCT ATCCGGGCAA TTGTGGTTCA 
GCGACCGTTG GAATAACGTT TCTCCTCTCA TACCTAGTTA TAAGCTTTTT GATAGTTATT 
AATATGTACA TTGCTGTCAT TCTCGAGAAC TATAGTCAGG CCACCGAGGA CGTGCAAGAG 
GGTCTAACCG ACGACGACTA CGACATGTAC TATGAGATCT GGCAGCAATT CGATCCGGAG 
GGCACCCAGT ACATACGCTA TGATCAGCTG TCCGAATTCC TGGACGTACT GGAGCCCCCG 
CTGCAGATCC ACAAACCGAA CAAGTACAAG ATCATATCGA TGGACATACC CATCTGTCGC 
GGTGACCTCA TGTACTGCGT CGACATCCTC GACGCCCTTA CGAAAGACTT CTTTGCGCGG 
AAGGGCAATC CGATAGAGGA GACGGGTGAG ATTGGTGAGA TAGCGGCCCG CCCGGATACG 
GAGGGCTACG AGCCCGTCTC ATCAACGCTG TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG 
CTAATCCAGC ACGCCTGGCG AAAGCACAAG GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG 
CCGGATACGG ATCATGGCGA TGGCGGTGAT CCGGATGCCG GGGACCCGGC GCCCGATGAA 
GCAACGGACG GCGATGCGCC CGCTGGTGGA GATGGTAGTG TTAACGGTAC TGCAGAAGGA 
GCTGCCGATG CCGATGAGAG TAATGTAAAT AGTCCGGGTG AGGATGCAGC GGCGGCGGCA 
GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC ACGACGACGG CGGGAAGTCC CGGAGCGGGT 
AGCGCCGGGC GACAGACCGC CGTTCTCGTG GAGAGCGACG GGTTCGTGAC GAAGAACGGC 
CACAAGGTGG TCATCCACTC GCGATCGCCG AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201722.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188651.1 Drosophila melanogaster paralytic (para), transcript variant BD, mRNA.

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CloneID	OFa03659
Clone ID Related Accession (Same CDS sequence)	NM_001201722.1
Accession Version	NM_001201722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform BD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGAGCATGGT GACAACGAAC TGGAGCTGGG CCACGACGAG 
ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG ATCAAGGAGC AGACGCAACT GGAGGTGGCC 
ATCGGGGATG GCATGGAATT CACGATACAC GGCGACATGA AGAACAACAA GCCGAAGAAA 
TCCAAATATC TAAATAACGC AACGGACGAC GACACTGCCA GCATTAACTC ATATGGTAGC 
CATAAGAATC GACCATTCAA GGACGAGAGC CACAAGGGCA GCGCCGAGAC GATGGAGGGC 
GAGGAGAAGC GCGACGCCAG CAAGGAGGAT TTAGGTCTCG ACGAGGAACT GGACGAGGAG 
GGCGAATGCG AGGAGGGCCC GCTCGACGGT GATATCATTA TTCATGCACA CGACGAGGAT 
ATACTCGATG AATATCCAGC TGATTGCTGC CCCGATTCGT ACTATAAGAA ATTTCCGATC 
TTAGCCGGTG ACGATGACTC GCCGTTCTGG CAAGGATGGG GCAATTTACG ACTGAAAACT 
TTTCAATTAA TTGAAAATAA ATATTTTGAA ACAGCTGTTA TCACTATGAT TTTAATGAGT 
AGCTTAGCTT TGGCATTAGA AGATGTACAT CTGCCACAAA GACCCATACT GCAGGATATT 
TTATACTATA TGGACAGAAT ATTTACGGTT ATATTCTTCT TGGAAATGTT AATCAAGTGG 
TTGGCGCTCG GCTTCAAAGT GTACTTCACC AACGCGTGGT GTTGGCTCGA TTTCGTGATT 
GTCATGGTCG TCGTTAATGC GCTGGTACAA GCTATACCGT CCATCTTCAA TGTGCTATTG 
GTGTGTCTAA TATTTTGGCT AATTTTTGCC ATAATGGGTG TACAGCTTTT TGCTGGAAAA 
TATTTTAAGT GCGAGGACAT GAATGGCACG AAGCTCAGCC ACGAGATCAT ACCAAATCGC 
AATGCCTGCG AGAGCGAGAA CTACACGTGG GTGAATTCAG CAATGAATTT CGATCATGTA 
GGTAACGCGT ATCTGTGCCT TTTCCAAGTG GCCACCTTCA AAGGCTGGAT ACAAATCATG 
AACGATGCTA TCGATTCACG AGAGGTGGAC AAGCAACCAA TTCGTGAAAC GAACATCTAC 
ATGTATTTAT ATTTCGTATT CTTCATCATA TTTGGATCAT TTTTCACACT CAATCTGTTC 
ATTGGTGTTA TCATTGATAA TTTTAATGAG CAAAAGAAAA AAGCAGGTGG ATCATTAGAA 
ATGTTCATGA CAGAAGATCA GAAAAAGTAC TATAATGCTA TGAAAAAGAT GGGCTCTAAA 
AAACCATTAA AAGCCATTCC AAGACCAAGG TGGCGACCAC AAGCAATAGT CTTTGAAATA 
GTAACCGATA AGAAATTCGA TATAATCATT ATGTTATTCA TTGGTCTGAA CATGTTCACC 
ATGACCCTCG ATCGTTACGA TGCGTCGGAC ACGTATAACG CGGTCCTAGA CTATCTCAAT 
GCGATATTCG TAGTTATTTT CAGTTCCGAA TGTCTATTAA AAATATTCGC TTTACGATAT 
CACTATTTTA TTGAGCCATG GAATTTATTT GATGTAGTAG TTGTCATTTT ATCCATCTTA 
GGTCTTGTAC TTAGCGATAT TATCGAGAAG TACTTCGTGT CGCCGACCCT GCTCCGAGTG 
GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT CGACTGGTGA AGGGAGCCAA GGGCATTCGG 
ACACTGCTCT TCGCGTTGGC CATGTCGCTG CCGGCCCTGT TCAACATCTG CCTGCTGCTG 
TTCCTGGTCA TGTTCATCTT TGCCATTTTC GGCATGTCGT TCTTCATGCA CGTGAAGGAG 
AAGAGCGGCA TCAACGACGT CTACAACTTC AAGACCTTTG GCCAGAGCAT GATCCTGCTC 
TTTCAGATGT CGACGTCAGC CGGTTGGGAT GGTGTACTGG ACGCCATTAT CAATGAGGAA 
GCATGCGATC CACCCGACAA CGACAAAGGC TATCCGGGCA ATTGTGGTTC AGCGACCGTT 
GGAATAACGT TTCTCCTCTC ATACCTAGTT ATAAGCTTTT TGATAGTTAT TAATATGTAC 
ATTGCTGTCA TTCTCGAGAA CTATAGTCAG GCCACCGAGG ACGTGCAAGA GGGTCTAACC 
GACGACGACT ACGACATGTA CTATGAGATC TGGCAGCAAT TCGATCCGGA GGGCACCCAG 
TACATACGCT ATGATCAGCT GTCCGAATTC CTGGACGTAC TGGAGCCCCC GCTGCAGATC 
CACAAACCGA ACAAGTACAA GATCATATCG ATGGACATAC CCATCTGTCG CGGTGACCTC 
ATGTACTGCG TCGACATCCT CGACGCCCTT ACGAAAGACT TCTTTGCGCG GAAGGGCAAT 
CCGATAGAGG AGACGGGTGA GATTGGTGAG ATAGCGGCCC GCCCGGATAC GGAGGGCTAC 
GAGCCCGTCT CATCAACGCT GTGGCGTCAG CGTGAGGAGT ACTGCGCCCG GCTAATCCAG 
CACGCCTGGC GAAAGCACAA GGCGCGCGGC GAGGGAGGTG GGTCCTTTGA GCCGGATACG 
GATCATGGCG ATGGCGGTGA TCCGGATGCC GGGGACCCGG CGCCCGATGA AGCAACGGAC 
GGCGATGCGC CCGCTGGTGG AGATGGTAGT GTTAACGGTA CTGCAGAAGG AGCTGCCGAT 
GCCGATGAGA GTAATGTAAA TAGTCCGGGT GAGGATGCAG CGGCGGCGGC AGCAGCAGCA 
GCAGCAGCGG CGGCGGCGGG CACGACGACG GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG 
CGACAGACCG CCGTTCTCGT GGAGAGCGAC GGGTTCGTGA CGAAGAACGG CCACAAGGTG 
GTCATCCACT CGCGATCGCC GAGCATCACG TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188651.1
CDS	269..6439
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201722.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant BD, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201722.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGAGCATGGT GACAACGAAC TGGAGCTGGG CCACGACGAG 
ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG ATCAAGGAGC AGACGCAACT GGAGGTGGCC 
ATCGGGGATG GCATGGAATT CACGATACAC GGCGACATGA AGAACAACAA GCCGAAGAAA 
TCCAAATATC TAAATAACGC AACGGACGAC GACACTGCCA GCATTAACTC ATATGGTAGC 
CATAAGAATC GACCATTCAA GGACGAGAGC CACAAGGGCA GCGCCGAGAC GATGGAGGGC 
GAGGAGAAGC GCGACGCCAG CAAGGAGGAT TTAGGTCTCG ACGAGGAACT GGACGAGGAG 
GGCGAATGCG AGGAGGGCCC GCTCGACGGT GATATCATTA TTCATGCACA CGACGAGGAT 
ATACTCGATG AATATCCAGC TGATTGCTGC CCCGATTCGT ACTATAAGAA ATTTCCGATC 
TTAGCCGGTG ACGATGACTC GCCGTTCTGG CAAGGATGGG GCAATTTACG ACTGAAAACT 
TTTCAATTAA TTGAAAATAA ATATTTTGAA ACAGCTGTTA TCACTATGAT TTTAATGAGT 
AGCTTAGCTT TGGCATTAGA AGATGTACAT CTGCCACAAA GACCCATACT GCAGGATATT 
TTATACTATA TGGACAGAAT ATTTACGGTT ATATTCTTCT TGGAAATGTT AATCAAGTGG 
TTGGCGCTCG GCTTCAAAGT GTACTTCACC AACGCGTGGT GTTGGCTCGA TTTCGTGATT 
GTCATGGTCG TCGTTAATGC GCTGGTACAA GCTATACCGT CCATCTTCAA TGTGCTATTG 
GTGTGTCTAA TATTTTGGCT AATTTTTGCC ATAATGGGTG TACAGCTTTT TGCTGGAAAA 
TATTTTAAGT GCGAGGACAT GAATGGCACG AAGCTCAGCC ACGAGATCAT ACCAAATCGC 
AATGCCTGCG AGAGCGAGAA CTACACGTGG GTGAATTCAG CAATGAATTT CGATCATGTA 
GGTAACGCGT ATCTGTGCCT TTTCCAAGTG GCCACCTTCA AAGGCTGGAT ACAAATCATG 
AACGATGCTA TCGATTCACG AGAGGTGGAC AAGCAACCAA TTCGTGAAAC GAACATCTAC 
ATGTATTTAT ATTTCGTATT CTTCATCATA TTTGGATCAT TTTTCACACT CAATCTGTTC 
ATTGGTGTTA TCATTGATAA TTTTAATGAG CAAAAGAAAA AAGCAGGTGG ATCATTAGAA 
ATGTTCATGA CAGAAGATCA GAAAAAGTAC TATAATGCTA TGAAAAAGAT GGGCTCTAAA 
AAACCATTAA AAGCCATTCC AAGACCAAGG TGGCGACCAC AAGCAATAGT CTTTGAAATA 
GTAACCGATA AGAAATTCGA TATAATCATT ATGTTATTCA TTGGTCTGAA CATGTTCACC 
ATGACCCTCG ATCGTTACGA TGCGTCGGAC ACGTATAACG CGGTCCTAGA CTATCTCAAT 
GCGATATTCG TAGTTATTTT CAGTTCCGAA TGTCTATTAA AAATATTCGC TTTACGATAT 
CACTATTTTA TTGAGCCATG GAATTTATTT GATGTAGTAG TTGTCATTTT ATCCATCTTA 
GGTCTTGTAC TTAGCGATAT TATCGAGAAG TACTTCGTGT CGCCGACCCT GCTCCGAGTG 
GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT CGACTGGTGA AGGGAGCCAA GGGCATTCGG 
ACACTGCTCT TCGCGTTGGC CATGTCGCTG CCGGCCCTGT TCAACATCTG CCTGCTGCTG 
TTCCTGGTCA TGTTCATCTT TGCCATTTTC GGCATGTCGT TCTTCATGCA CGTGAAGGAG 
AAGAGCGGCA TCAACGACGT CTACAACTTC AAGACCTTTG GCCAGAGCAT GATCCTGCTC 
TTTCAGATGT CGACGTCAGC CGGTTGGGAT GGTGTACTGG ACGCCATTAT CAATGAGGAA 
GCATGCGATC CACCCGACAA CGACAAAGGC TATCCGGGCA ATTGTGGTTC AGCGACCGTT 
GGAATAACGT TTCTCCTCTC ATACCTAGTT ATAAGCTTTT TGATAGTTAT TAATATGTAC 
ATTGCTGTCA TTCTCGAGAA CTATAGTCAG GCCACCGAGG ACGTGCAAGA GGGTCTAACC 
GACGACGACT ACGACATGTA CTATGAGATC TGGCAGCAAT TCGATCCGGA GGGCACCCAG 
TACATACGCT ATGATCAGCT GTCCGAATTC CTGGACGTAC TGGAGCCCCC GCTGCAGATC 
CACAAACCGA ACAAGTACAA GATCATATCG ATGGACATAC CCATCTGTCG CGGTGACCTC 
ATGTACTGCG TCGACATCCT CGACGCCCTT ACGAAAGACT TCTTTGCGCG GAAGGGCAAT 
CCGATAGAGG AGACGGGTGA GATTGGTGAG ATAGCGGCCC GCCCGGATAC GGAGGGCTAC 
GAGCCCGTCT CATCAACGCT GTGGCGTCAG CGTGAGGAGT ACTGCGCCCG GCTAATCCAG 
CACGCCTGGC GAAAGCACAA GGCGCGCGGC GAGGGAGGTG GGTCCTTTGA GCCGGATACG 
GATCATGGCG ATGGCGGTGA TCCGGATGCC GGGGACCCGG CGCCCGATGA AGCAACGGAC 
GGCGATGCGC CCGCTGGTGG AGATGGTAGT GTTAACGGTA CTGCAGAAGG AGCTGCCGAT 
GCCGATGAGA GTAATGTAAA TAGTCCGGGT GAGGATGCAG CGGCGGCGGC AGCAGCAGCA 
GCAGCAGCGG CGGCGGCGGG CACGACGACG GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG 
CGACAGACCG CCGTTCTCGT GGAGAGCGAC GGGTTCGTGA CGAAGAACGG CCACAAGGTG 
GTCATCCACT CGCGATCGCC GAGCATCACG TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201720.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188649.1 Drosophila melanogaster paralytic (para), transcript variant BB, mRNA.

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CloneID	OFa03660
Clone ID Related Accession (Same CDS sequence)	NM_001201720.1
Accession Version	NM_001201720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform BB
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGCAAAGGC GTTTGTCGTT GTATATCTGC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGGACGAC 
GACACTGCCA GCATTAACTC ATATGGTAGC CATAAGAATC GACCATTCAA GGACGAGAGC 
CACAAGGGCA GCGCCGAGAC GATGGAGGGC GAGGAGAAGC GCGACGCCAG CAAGGAGGAT 
TTAGGTCTCG ACGAGGAACT GGACGAGGAG GGCGAATGCG AGGAGGGCCC GCTCGACGGT 
GATATCATTA TTCATGCACA CGACGAGGAT ATACTCGATG AATATCCAGC TGATTGCTGC 
CCCGATTCGT ACTATAAGAA ATTTCCGATC TTAGCCGGTG ACGATGACTC GCCGTTCTGG 
CAAGGATGGG GCAATTTACG ACTGAAAACT TTTCAATTAA TTGAAAATAA ATATTTTGAA 
ACAGCTGTTA TCACTATGAT TTTAATGAGT AGCTTAGCTT TGGCATTAGA AGATGTACAT 
CTGCCACAAA GACCCATACT GCAGGATATT TTATACTATA TGGACAGAAT ATTTACGGTT 
ATATTCTTCT TGGAAATGTT AATCAAGTGG TTGGCGCTCG GCTTCAAAGT GTACTTCACC 
AACGCGTGGT GTTGGCTCGA TTTCGTGATT GTCATGGTCG TCGTTAATGC GCTGGTACAA 
GCTATACCGT CCATCTTCAA TGTGCTATTG GTGTGTCTAA TATTTTGGCT AATTTTTGCC 
ATAATGGGTG TACAGCTTTT TGCTGGAAAA TATTTTAAGT GCGAGGACAT GAATGGCACG 
AAGCTCAGCC ACGAGATCAT ACCAAATCGC AATGCCTGCG AGAGCGAGAA CTACACGTGG 
GTGAATTCAG CAATGAATTT CGATCATGTA GGTAACGCGT ATCTGTGCCT TTTCCAAGTG 
GCCACCTTCA AAGGCTGGAT ACAAATCATG AACGATGCTA TCGATTCACG AGAGGTGGAC 
AAGCAACCAA TTCGTGAAAC GAACATCTAC ATGTATTTAT ATTTCGTATT CTTCATCATA 
TTTGGATCAT TTTTCACACT CAATCTGTTC ATTGGTGTTA TCATTGATAA TTTTAATGAG 
CAAAAGAAAA AAGCAGGTGG ATCATTAGAA ATGTTCATGA CAGAAGATCA GAAAAAGTAC 
TATAATGCTA TGAAAAAGAT GGGCTCTAAA AAACCATTAA AAGCCATTCC AAGACCAAGG 
TGGCGACCAC AAGCAATAGT CTTTGAAATA GTAACCGATA AGAAATTCGA TATAATCATT 
ATGTTATTCA TTGGTCTGAA CATGTTCACC ATGACCCTCG ATCGTTACGA TGCGTCGGAC 
ACGTATAACG CGGTCCTAGA CTATCTCAAT GCGATATTCG TAGTTATTTT CAGTTCCGAA 
TGTCTATTAA AAATATTCGC TTTACGATAT CACTATTTTA TTGAGCCATG GAATTTATTT 
GATGTAGTAG TTGTCATTTT ATCCATCTTA GGTCTTGTAC TTAGCGATAT TATCGAGAAG 
TACTTCGTGT CGCCGACCCT GCTCCGAGTG GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT 
CGACTGGTGA AGGGAGCCAA GGGCATTCGG ACACTGCTCT TCGCGTTGGC CATGTCGCTG 
CCGGCCCTGT TCAACATCTG CCTGCTGCTG TTCCTGGTCA TGTTCATCTT TGCCATTTTC 
GGCATGTCGT TCTTCATGCA CGTGAAGGAG AAGAGCGGCA TCAACGACGT CTACAACTTC 
AAGACCTTTG GCCAGAGCAT GATCCTGCTC TTTCAGATGT CGACGTCAGC CGGTTGGGAT 
GGTGTACTGG ACGCCATTAT CAATGAGGAA GCATGCGATC CACCCGACAA CGACAAAGGC 
TATCCGGGCA ATTGTGGTTC AGCGACCGTT GGAATAACGT TTCTCCTCTC ATACCTAGTT 
ATAAGCTTTT TGATAGTTAT TAATATGTAC ATTGCTGTCA TTCTCGAGAA CTATAGTCAG 
GCCACCGAGG ACGTGCAAGA GGGTCTAACC GACGACGACT ACGACATGTA CTATGAGATC 
TGGCAGCAAT TCGATCCGGA GGGCACCCAG TACATACGCT ATGATCAGCT GTCCGAATTC 
CTGGACGTAC TGGAGCCCCC GCTGCAGATC CACAAACCGA ACAAGTACAA GATCATATCG 
ATGGACATAC CCATCTGTCG CGGTGACCTC ATGTACTGCG TCGACATCCT CGACGCCCTT 
ACGAAAGACT TCTTTGCGCG GAAGGGCAAT CCGATAGAGG AGACGGGTGA GATTGGTGAG 
ATAGCGGCCC GCCCGGATAC GGAGGGCTAC GAGCCCGTCT CATCAACGCT GTGGCGTCAG 
CGTGAGGAGT ACTGCGCCCG GCTAATCCAG CACGCCTGGC GAAAGCACAA GGCGCGCGGC 
GAGGGAGGTG GGTCCTTTGA GCCGGATACG GATCATGGCG ATGGCGGTGA TCCGGATGCC 
GGGGACCCGG CGCCCGATGA AGCAACGGAC GGCGATGCGC CCGCTGGTGG AGATGGTAGT 
GTTAACGGTA CTGCAGAAGG AGCTGCCGAT GCCGATGAGA GTAATGTAAA TAGTCCGGGT 
GAGGATGCAG CGGCGGCGGC AGCAGCAGCA GCAGCAGCGG CGGCGGCGGG CACGACGACG 
GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG CGACAGACCG CCGTTCTCGT GGAGAGCGAC 
GGGTTCGTGA CGAAGAACGG CCACAAGGTG GTCATCCACT CGCGATCGCC GAGCATCACG 
TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188649.1
CDS	269..6469
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201720.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant BB, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201720.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGCAAAGGC GTTTGTCGTT GTATATCTGC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGGACGAC 
GACACTGCCA GCATTAACTC ATATGGTAGC CATAAGAATC GACCATTCAA GGACGAGAGC 
CACAAGGGCA GCGCCGAGAC GATGGAGGGC GAGGAGAAGC GCGACGCCAG CAAGGAGGAT 
TTAGGTCTCG ACGAGGAACT GGACGAGGAG GGCGAATGCG AGGAGGGCCC GCTCGACGGT 
GATATCATTA TTCATGCACA CGACGAGGAT ATACTCGATG AATATCCAGC TGATTGCTGC 
CCCGATTCGT ACTATAAGAA ATTTCCGATC TTAGCCGGTG ACGATGACTC GCCGTTCTGG 
CAAGGATGGG GCAATTTACG ACTGAAAACT TTTCAATTAA TTGAAAATAA ATATTTTGAA 
ACAGCTGTTA TCACTATGAT TTTAATGAGT AGCTTAGCTT TGGCATTAGA AGATGTACAT 
CTGCCACAAA GACCCATACT GCAGGATATT TTATACTATA TGGACAGAAT ATTTACGGTT 
ATATTCTTCT TGGAAATGTT AATCAAGTGG TTGGCGCTCG GCTTCAAAGT GTACTTCACC 
AACGCGTGGT GTTGGCTCGA TTTCGTGATT GTCATGGTCG TCGTTAATGC GCTGGTACAA 
GCTATACCGT CCATCTTCAA TGTGCTATTG GTGTGTCTAA TATTTTGGCT AATTTTTGCC 
ATAATGGGTG TACAGCTTTT TGCTGGAAAA TATTTTAAGT GCGAGGACAT GAATGGCACG 
AAGCTCAGCC ACGAGATCAT ACCAAATCGC AATGCCTGCG AGAGCGAGAA CTACACGTGG 
GTGAATTCAG CAATGAATTT CGATCATGTA GGTAACGCGT ATCTGTGCCT TTTCCAAGTG 
GCCACCTTCA AAGGCTGGAT ACAAATCATG AACGATGCTA TCGATTCACG AGAGGTGGAC 
AAGCAACCAA TTCGTGAAAC GAACATCTAC ATGTATTTAT ATTTCGTATT CTTCATCATA 
TTTGGATCAT TTTTCACACT CAATCTGTTC ATTGGTGTTA TCATTGATAA TTTTAATGAG 
CAAAAGAAAA AAGCAGGTGG ATCATTAGAA ATGTTCATGA CAGAAGATCA GAAAAAGTAC 
TATAATGCTA TGAAAAAGAT GGGCTCTAAA AAACCATTAA AAGCCATTCC AAGACCAAGG 
TGGCGACCAC AAGCAATAGT CTTTGAAATA GTAACCGATA AGAAATTCGA TATAATCATT 
ATGTTATTCA TTGGTCTGAA CATGTTCACC ATGACCCTCG ATCGTTACGA TGCGTCGGAC 
ACGTATAACG CGGTCCTAGA CTATCTCAAT GCGATATTCG TAGTTATTTT CAGTTCCGAA 
TGTCTATTAA AAATATTCGC TTTACGATAT CACTATTTTA TTGAGCCATG GAATTTATTT 
GATGTAGTAG TTGTCATTTT ATCCATCTTA GGTCTTGTAC TTAGCGATAT TATCGAGAAG 
TACTTCGTGT CGCCGACCCT GCTCCGAGTG GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT 
CGACTGGTGA AGGGAGCCAA GGGCATTCGG ACACTGCTCT TCGCGTTGGC CATGTCGCTG 
CCGGCCCTGT TCAACATCTG CCTGCTGCTG TTCCTGGTCA TGTTCATCTT TGCCATTTTC 
GGCATGTCGT TCTTCATGCA CGTGAAGGAG AAGAGCGGCA TCAACGACGT CTACAACTTC 
AAGACCTTTG GCCAGAGCAT GATCCTGCTC TTTCAGATGT CGACGTCAGC CGGTTGGGAT 
GGTGTACTGG ACGCCATTAT CAATGAGGAA GCATGCGATC CACCCGACAA CGACAAAGGC 
TATCCGGGCA ATTGTGGTTC AGCGACCGTT GGAATAACGT TTCTCCTCTC ATACCTAGTT 
ATAAGCTTTT TGATAGTTAT TAATATGTAC ATTGCTGTCA TTCTCGAGAA CTATAGTCAG 
GCCACCGAGG ACGTGCAAGA GGGTCTAACC GACGACGACT ACGACATGTA CTATGAGATC 
TGGCAGCAAT TCGATCCGGA GGGCACCCAG TACATACGCT ATGATCAGCT GTCCGAATTC 
CTGGACGTAC TGGAGCCCCC GCTGCAGATC CACAAACCGA ACAAGTACAA GATCATATCG 
ATGGACATAC CCATCTGTCG CGGTGACCTC ATGTACTGCG TCGACATCCT CGACGCCCTT 
ACGAAAGACT TCTTTGCGCG GAAGGGCAAT CCGATAGAGG AGACGGGTGA GATTGGTGAG 
ATAGCGGCCC GCCCGGATAC GGAGGGCTAC GAGCCCGTCT CATCAACGCT GTGGCGTCAG 
CGTGAGGAGT ACTGCGCCCG GCTAATCCAG CACGCCTGGC GAAAGCACAA GGCGCGCGGC 
GAGGGAGGTG GGTCCTTTGA GCCGGATACG GATCATGGCG ATGGCGGTGA TCCGGATGCC 
GGGGACCCGG CGCCCGATGA AGCAACGGAC GGCGATGCGC CCGCTGGTGG AGATGGTAGT 
GTTAACGGTA CTGCAGAAGG AGCTGCCGAT GCCGATGAGA GTAATGTAAA TAGTCCGGGT 
GAGGATGCAG CGGCGGCGGC AGCAGCAGCA GCAGCAGCGG CGGCGGCGGG CACGACGACG 
GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG CGACAGACCG CCGTTCTCGT GGAGAGCGAC 
GGGTTCGTGA CGAAGAACGG CCACAAGGTG GTCATCCACT CGCGATCGCC GAGCATCACG 
TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201700.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188629.1 Drosophila melanogaster paralytic (para), transcript variant AH, mRNA.

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CloneID	OFa03661
Clone ID Related Accession (Same CDS sequence)	NM_001201700.1
Accession Version	NM_001201700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AH
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGCAAAGGC GTTTGTCGTT GTATATCTGC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGGACGAC 
GACACTGCCA GCATTAACTC ATATGGTAGC CATAAGAATC GACCATTCAA GGACGAGAGC 
CACAAGGGCA GCGCCGAGAC GATGGAGGGC GAGGAGAAGC GCGACGCCAG CAAGGAGGAT 
TTAGGTCTCG ACGAGGAACT GGACGAGGAG GGCGAATGCG AGGAGGGCCC GCTCGACGGT 
GATATCATTA TTCATGCACA CGACGAGGAT ATACTCGATG AATATCCAGC TGATTGCTGC 
CCCGATTCGT ACTATAAGAA ATTTCCGATC TTAGCCGGTG ACGATGACTC GCCGTTCTGG 
CAAGGATGGG GCAATTTACG ACTGAAAACT TTTCAATTAA TTGAAAATAA ATATTTTGAA 
ACAGCTGTTA TCACTATGAT TTTAATGAGT AGCTTAGCTT TGGCATTAGA AGATGTACAT 
CTGCCACAAA GACCCATACT GCAGGATATT TTATACTATA TGGACAGAAT ATTTACGGTT 
ATATTCTTCT TGGAAATGTT AATCAAGTGG TTGGCGCTCG GCTTCAAAGT GTACTTCACC 
AACGCGTGGT GTTGGCTCGA TTTCGTGATT GTCATGGTAT CGCTTATCAA CTTCGTTGCT 
TCACTTGTTG GAGCTGGTGG TATTCAAGCC TTCAAGACTA TGCGAACGTT AAGAGCACTG 
AGACCACTAC GTGCCATGTC CCGTATGCAG GGCATGAGGG TCGTCGTTAA TGCGCTGGTA 
CAAGCTATAC CGTCCATCTT CAATGTGCTA TTGGTGTGTC TAATATTTTG GCTAATTTTT 
GCCATAATGG GTGTACAGCT TTTTGCTGGA AAATATTTTA AGTGCGAGGA CATGAATGGC 
ACGAAGCTCA GCCACGAGAT CATACCAAAT CGCAATGCCT GCGAGAGCGA GAACTACACG 
TGGGTGAATT CAGCAATGAA TTTCGATCAT GTAGGTAACG CGTATCTGTG CCTTTTCCAA 
GTGGCCACCT TCAAAGGCTG GATACAAATC ATGAACGATG CTATCGATTC ACGAGAGGTG 
GACAAGCAAC CAATTCGTGA AACGAACATC TACATGTATT TATATTTCGT ATTCTTCATC 
ATATTTGGAT CATTTTTCAC ACTCAATCTG TTCATTGGTG TTATCATTGA TAATTTTAAT 
GAGCAAAAGA AAAAAGCAGG TGGATCATTA GAAATGTTCA TGACAGAAGA TCAGAAAAAG 
TACTATAATG CTATGAAAAA GATGGGCTCT AAAAAACCAT TAAAAGCCAT TCCAAGACCA 
AGGTGGCGAC CACAAGCAAT AGTCTTTGAA ATAGTAACCG ATAAGAAATT CGATATAATC 
ATTATGTTAT TCATTGGTCT GAACATGTTC ACCATGACCC TCGATCGTTA CGATGCGTCG 
GACACGTATA ACGCGGTCCT AGACTATCTC AATGCGATAT TCGTAGTTAT TTTCAGTTCC 
GAATGTCTAT TAAAAATATT CGCTTTACGA TATCACTATT TTATTGAGCC ATGGAATTTA 
TTTGATGTAG TAGTTGTCAT TTTATCCATC TTAGGTCTTG TACTTAGCGA TATTATCGAG 
AAGTACTTCG TGTCGCCGAC CCTGCTCCGA GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC 
CTTCGACTGG TGAAGGGAGC CAAGGGCATT CGGACACTGC TCTTCGCGTT GGCCATGTCG 
CTGCCGGCCC TGTTCAACAT CTGCCTGCTG CTGTTCCTGG TCATGTTCAT CTTTGCCATT 
TTCGGCATGT CGTTCTTCAT GCACGTGAAG GAGAAGAGCG GCATCAACGA CGTCTACAAC 
TTCAAGACCT TTGGCCAGAG CATGATCCTG CTCTTTCAGA TGTCGACGTC AGCCGGTTGG 
GATGGTGTAC TGGACGCCAT TATCAATGAG GAAGCATGCG ATCCACCCGA CAACGACAAA 
GGCTATCCGG GCAATTGTGG TTCAGCGACC GTTGGAATAA CGTTTCTCCT CTCATACCTA 
GTTATAAGCT TTTTGATAGT TATTAATATG TACATTGCTG TCATTCTCGA GAACTATAGT 
CAGGCCACCG AGGACGTGCA AGAGGGTCTA ACCGACGACG ACTACGACAT GTACTATGAG 
ATCTGGCAGC AATTCGATCC GGAGGGCACC CAGTACATAC GCTATGATCA GCTGTCCGAA 
TTCCTGGACG TACTGGAGCC CCCGCTGCAG ATCCACAAAC CGAACAAGTA CAAGATCATA 
TCGATGGACA TACCCATCTG TCGCGGTGAC CTCATGTACT GCGTCGACAT CCTCGACGCC 
CTTACGAAAG ACTTCTTTGC GCGGAAGGGC AATCCGATAG AGGAGACGGG TGAGATTGGT 
GAGATAGCGG CCCGCCCGGA TACGGAGGGC TACGAGCCCG TCTCATCAAC GCTGTGGCGT 
CAGCGTGAGG AGTACTGCGC CCGGCTAATC CAGCACGCCT GGCGAAAGCA CAAGGCGCGC 
GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT ACGGATCATG GCGATGGCGG TGATCCGGAT 
GCCGGGGACC CGGCGCCCGA TGAAGCAACG GACGGCGATG CGCCCGCTGG TGGAGATGGT 
AGTGTTAACG GTACTGCAGA AGGAGCTGCC GATGCCGATG AGAGTAATGT AAATAGTCCG 
GGTGAGGATG CAGCGGCGGC GGCAGCAGCA GCAGCAGCAG CGGCGGCGGC GGGCACGACG 
ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC GGGCGACAGA CCGCCGTTCT CGTGGAGAGC 
GACGGGTTCG TGACGAAGAA CGGCCACAAG GTGGTCATCC ACTCGCGATC GCCGAGCATC 
ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188629.1
CDS	269..6592
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201700.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AH, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201700.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGCAAAGGC GTTTGTCGTT GTATATCTGC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGGACGAC 
GACACTGCCA GCATTAACTC ATATGGTAGC CATAAGAATC GACCATTCAA GGACGAGAGC 
CACAAGGGCA GCGCCGAGAC GATGGAGGGC GAGGAGAAGC GCGACGCCAG CAAGGAGGAT 
TTAGGTCTCG ACGAGGAACT GGACGAGGAG GGCGAATGCG AGGAGGGCCC GCTCGACGGT 
GATATCATTA TTCATGCACA CGACGAGGAT ATACTCGATG AATATCCAGC TGATTGCTGC 
CCCGATTCGT ACTATAAGAA ATTTCCGATC TTAGCCGGTG ACGATGACTC GCCGTTCTGG 
CAAGGATGGG GCAATTTACG ACTGAAAACT TTTCAATTAA TTGAAAATAA ATATTTTGAA 
ACAGCTGTTA TCACTATGAT TTTAATGAGT AGCTTAGCTT TGGCATTAGA AGATGTACAT 
CTGCCACAAA GACCCATACT GCAGGATATT TTATACTATA TGGACAGAAT ATTTACGGTT 
ATATTCTTCT TGGAAATGTT AATCAAGTGG TTGGCGCTCG GCTTCAAAGT GTACTTCACC 
AACGCGTGGT GTTGGCTCGA TTTCGTGATT GTCATGGTAT CGCTTATCAA CTTCGTTGCT 
TCACTTGTTG GAGCTGGTGG TATTCAAGCC TTCAAGACTA TGCGAACGTT AAGAGCACTG 
AGACCACTAC GTGCCATGTC CCGTATGCAG GGCATGAGGG TCGTCGTTAA TGCGCTGGTA 
CAAGCTATAC CGTCCATCTT CAATGTGCTA TTGGTGTGTC TAATATTTTG GCTAATTTTT 
GCCATAATGG GTGTACAGCT TTTTGCTGGA AAATATTTTA AGTGCGAGGA CATGAATGGC 
ACGAAGCTCA GCCACGAGAT CATACCAAAT CGCAATGCCT GCGAGAGCGA GAACTACACG 
TGGGTGAATT CAGCAATGAA TTTCGATCAT GTAGGTAACG CGTATCTGTG CCTTTTCCAA 
GTGGCCACCT TCAAAGGCTG GATACAAATC ATGAACGATG CTATCGATTC ACGAGAGGTG 
GACAAGCAAC CAATTCGTGA AACGAACATC TACATGTATT TATATTTCGT ATTCTTCATC 
ATATTTGGAT CATTTTTCAC ACTCAATCTG TTCATTGGTG TTATCATTGA TAATTTTAAT 
GAGCAAAAGA AAAAAGCAGG TGGATCATTA GAAATGTTCA TGACAGAAGA TCAGAAAAAG 
TACTATAATG CTATGAAAAA GATGGGCTCT AAAAAACCAT TAAAAGCCAT TCCAAGACCA 
AGGTGGCGAC CACAAGCAAT AGTCTTTGAA ATAGTAACCG ATAAGAAATT CGATATAATC 
ATTATGTTAT TCATTGGTCT GAACATGTTC ACCATGACCC TCGATCGTTA CGATGCGTCG 
GACACGTATA ACGCGGTCCT AGACTATCTC AATGCGATAT TCGTAGTTAT TTTCAGTTCC 
GAATGTCTAT TAAAAATATT CGCTTTACGA TATCACTATT TTATTGAGCC ATGGAATTTA 
TTTGATGTAG TAGTTGTCAT TTTATCCATC TTAGGTCTTG TACTTAGCGA TATTATCGAG 
AAGTACTTCG TGTCGCCGAC CCTGCTCCGA GTGGTGCGTG TGGCGAAAGT GGGCCGTGTC 
CTTCGACTGG TGAAGGGAGC CAAGGGCATT CGGACACTGC TCTTCGCGTT GGCCATGTCG 
CTGCCGGCCC TGTTCAACAT CTGCCTGCTG CTGTTCCTGG TCATGTTCAT CTTTGCCATT 
TTCGGCATGT CGTTCTTCAT GCACGTGAAG GAGAAGAGCG GCATCAACGA CGTCTACAAC 
TTCAAGACCT TTGGCCAGAG CATGATCCTG CTCTTTCAGA TGTCGACGTC AGCCGGTTGG 
GATGGTGTAC TGGACGCCAT TATCAATGAG GAAGCATGCG ATCCACCCGA CAACGACAAA 
GGCTATCCGG GCAATTGTGG TTCAGCGACC GTTGGAATAA CGTTTCTCCT CTCATACCTA 
GTTATAAGCT TTTTGATAGT TATTAATATG TACATTGCTG TCATTCTCGA GAACTATAGT 
CAGGCCACCG AGGACGTGCA AGAGGGTCTA ACCGACGACG ACTACGACAT GTACTATGAG 
ATCTGGCAGC AATTCGATCC GGAGGGCACC CAGTACATAC GCTATGATCA GCTGTCCGAA 
TTCCTGGACG TACTGGAGCC CCCGCTGCAG ATCCACAAAC CGAACAAGTA CAAGATCATA 
TCGATGGACA TACCCATCTG TCGCGGTGAC CTCATGTACT GCGTCGACAT CCTCGACGCC 
CTTACGAAAG ACTTCTTTGC GCGGAAGGGC AATCCGATAG AGGAGACGGG TGAGATTGGT 
GAGATAGCGG CCCGCCCGGA TACGGAGGGC TACGAGCCCG TCTCATCAAC GCTGTGGCGT 
CAGCGTGAGG AGTACTGCGC CCGGCTAATC CAGCACGCCT GGCGAAAGCA CAAGGCGCGC 
GGCGAGGGAG GTGGGTCCTT TGAGCCGGAT ACGGATCATG GCGATGGCGG TGATCCGGAT 
GCCGGGGACC CGGCGCCCGA TGAAGCAACG GACGGCGATG CGCCCGCTGG TGGAGATGGT 
AGTGTTAACG GTACTGCAGA AGGAGCTGCC GATGCCGATG AGAGTAATGT AAATAGTCCG 
GGTGAGGATG CAGCGGCGGC GGCAGCAGCA GCAGCAGCAG CGGCGGCGGC GGGCACGACG 
ACGGCGGGAA GTCCCGGAGC GGGTAGCGCC GGGCGACAGA CCGCCGTTCT CGTGGAGAGC 
GACGGGTTCG TGACGAAGAA CGGCCACAAG GTGGTCATCC ACTCGCGATC GCCGAGCATC 
ACGTCGCGCA CGGCGGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201702.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188631.1 Drosophila melanogaster paralytic (para), transcript variant AJ, mRNA.

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CloneID	OFa03662
Clone ID Related Accession (Same CDS sequence)	NM_001201702.1
Accession Version	NM_001201702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AJ
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGCAAAGGC GTTTGTCGTT GTATATCTGC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGATGATT 
GGCAACTCAA TTAACCACCA AGACAATAGA CTGGAACACG AGCTAAACCA TAGAGGTTTG 
TCCTTACAGG ACGACGACAC TGCCAGCATT AACTCATATG GTAGCCATAA GAATCGACCA 
TTCAAGGACG AGAGCCACAA GGGCAGCGCC GAGACGATGG AGGGCGAGGA GAAGCGCGAC 
GCCAGCAAGG AGGATTTAGG TCTCGACGAG GAACTGGACG AGGAGGGCGA ATGCGAGGAG 
GGCCCGCTCG ACGGTGATAT CATTATTCAT GCACACGACG AGGATATACT CGATGAATAT 
CCAGCTGATT GCTGCCCCGA TTCGTACTAT AAGAAATTTC CGATCTTAGC CGGTGACGAT 
GACTCGCCGT TCTGGCAAGG ATGGGGCAAT TTACGACTGA AAACTTTTCA ATTAATTGAA 
AATAAATATT TTGAAACAGC TGTTATCACT ATGATTTTAA TGAGTAGCTT AGCTTTGGCA 
TTAGAAGATG TACATCTGCC ACAAAGACCC ATACTGCAGG ATATTTTATA CTATATGGAC 
AGAATATTTA CGGTTATATT CTTCTTGGAA ATGTTAATCA AGTGGTTGGC GCTCGGCTTC 
AAAGTGTACT TCACCAACGC GTGGTGTTGG CTCGATTTCG TGATTGTCAT GGTATCGCTT 
ATCAACTTCG TTGCTTCACT TGTTGGAGCT GGTGGTATTC AAGCCTTCAA GACTATGCGA 
ACGTTAAGAG CACTGAGACC ACTACGTGCC ATGTCCCGTA TGCAGGGCAT GAGGGTCGTC 
GTTAATGCGC TGGTACAAGC TATACCGTCC ATCTTCAATG TGCTATTGGT GTGTCTAATA 
TTTTGGCTAA TTTTTGCCAT AATGGGTGTA CAGCTTTTTG CTGGAAAATA TTTTAAGTGC 
GAGGACATGA ATGGCACGAA GCTCAGCCAC GAGATCATAC CAAATCGCAA TGCCTGCGAG 
AGCGAGAACT ACACGTGGGT GAATTCAGCA ATGAATTTCG ATCATGTAGG TAACGCGTAT 
CTGTGCCTTT TCCAAGTGGC CACCTTCAAA GGCTGGATAC AAATCATGAA CGATGCTATC 
GATTCACGAG AGGTGGACAA GCAACCAATT CGTGAAACGA ACATCTACAT GTATTTATAT 
TTCGTATTCT TCATCATATT TGGATCATTT TTCACACTCA ATCTGTTCAT TGGTGTTATC 
ATTGATAATT TTAATGAGCA AAAGAAAAAA GCAGGTGGAT CATTAGAAAT GTTCATGACA 
GAAGATCAGA AAAAGTACTA TAATGCTATG AAAAAGATGG GCTCTAAAAA ACCATTAAAA 
GCCATTCCAA GACCAAGGTG GCGACCACAA GCAATAGTCT TTGAAATAGT AACCGATAAG 
AAATTCGATA TAATCATTAT GTTATTCATT GGTCTGAACA TGTTCACCAT GACCCTCGAT 
CGTTACGATG CGTCGGACAC GTATAACGCG GTCCTAGACT ATCTCAATGC GATATTCGTA 
GTTATTTTCA GTTCCGAATG TCTATTAAAA ATATTCGCTT TACGATATCA CTATTTTATT 
GAGCCATGGA ATTTATTTGA TGTAGTAGTT GTCATTTTAT CCATCTTAGG TCTTGTACTT 
AGCGATATTA TCGAGAAGTA CTTCGTGTCG CCGACCCTGC TCCGAGTGGT GCGTGTGGCG 
AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG GGAGCCAAGG GCATTCGGAC ACTGCTCTTC 
GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC AACATCTGCC TGCTGCTGTT CCTGGTCATG 
TTCATCTTTG CCATTTTCGG CATGTCGTTC TTCATGCACG TGAAGGAGAA GAGCGGCATC 
AACGACGTCT ACAACTTCAA GACCTTTGGC CAGAGCATGA TCCTGCTCTT TCAGATGTCG 
ACGTCAGCCG GTTGGGATGG TGTACTGGAC GCCATTATCA ATGAGGAAGC ATGCGATCCA 
CCCGACAACG ACAAAGGCTA TCCGGGCAAT TGTGGTTCAG CGACCGTTGG AATAACGTTT 
CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG ATAGTTATTA ATATGTACAT TGCTGTCATT 
CTCGAGAACT ATAGTCAGGC CACCGAGGAC GTGCAAGAGG GTCTAACCGA CGACGACTAC 
GACATGTACT ATGAGATCTG GCAGCAATTC GATCCGGAGG GCACCCAGTA CATACGCTAT 
GATCAGCTGT CCGAATTCCT GGACGTACTG GAGCCCCCGC TGCAGATCCA CAAACCGAAC 
AAGTACAAGA TCATATCGAT GGACATACCC ATCTGTCGCG GTGACCTCAT GTACTGCGTC 
GACATCCTCG ACGCCCTTAC GAAAGACTTC TTTGCGCGGA AGGGCAATCC GATAGAGGAG 
ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC CCGGATACGG AGGGCTACGA GCCCGTCTCA 
TCAACGCTGT GGCGTCAGCG TGAGGAGTAC TGCGCCCGGC TAATCCAGCA CGCCTGGCGA 
AAGCACAAGG CGCGCGGCGA GGGAGGTGGG TCCTTTGAGC CGGATACGGA TCATGGCGAT 
GGCGGTGATC CGGATGCCGG GGACCCGGCG CCCGATGAAG CAACGGACGG CGATGCGCCC 
GCTGGTGGAG ATGGTAGTGT TAACGGTACT GCAGAAGGAG CTGCCGATGC CGATGAGAGT 
AATGTAAATA GTCCGGGTGA GGATGCAGCG GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG 
GCGGCGGGCA CGACGACGGC GGGAAGTCCC GGAGCGGGTA GCGCCGGGCG ACAGACCGCC 
GTTCTCGTGG AGAGCGACGG GTTCGTGACG AAGAACGGCC ACAAGGTGGT CATCCACTCG 
CGATCGCCGA GCATCACGTC GCGCACGGCG GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188631.1
CDS	269..6667
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATMIGNSINHQDNRLEHELNHRGLSLQDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201702.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AJ, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201702.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGCAAAGGC GTTTGTCGTT GTATATCTGC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGATGATT 
GGCAACTCAA TTAACCACCA AGACAATAGA CTGGAACACG AGCTAAACCA TAGAGGTTTG 
TCCTTACAGG ACGACGACAC TGCCAGCATT AACTCATATG GTAGCCATAA GAATCGACCA 
TTCAAGGACG AGAGCCACAA GGGCAGCGCC GAGACGATGG AGGGCGAGGA GAAGCGCGAC 
GCCAGCAAGG AGGATTTAGG TCTCGACGAG GAACTGGACG AGGAGGGCGA ATGCGAGGAG 
GGCCCGCTCG ACGGTGATAT CATTATTCAT GCACACGACG AGGATATACT CGATGAATAT 
CCAGCTGATT GCTGCCCCGA TTCGTACTAT AAGAAATTTC CGATCTTAGC CGGTGACGAT 
GACTCGCCGT TCTGGCAAGG ATGGGGCAAT TTACGACTGA AAACTTTTCA ATTAATTGAA 
AATAAATATT TTGAAACAGC TGTTATCACT ATGATTTTAA TGAGTAGCTT AGCTTTGGCA 
TTAGAAGATG TACATCTGCC ACAAAGACCC ATACTGCAGG ATATTTTATA CTATATGGAC 
AGAATATTTA CGGTTATATT CTTCTTGGAA ATGTTAATCA AGTGGTTGGC GCTCGGCTTC 
AAAGTGTACT TCACCAACGC GTGGTGTTGG CTCGATTTCG TGATTGTCAT GGTATCGCTT 
ATCAACTTCG TTGCTTCACT TGTTGGAGCT GGTGGTATTC AAGCCTTCAA GACTATGCGA 
ACGTTAAGAG CACTGAGACC ACTACGTGCC ATGTCCCGTA TGCAGGGCAT GAGGGTCGTC 
GTTAATGCGC TGGTACAAGC TATACCGTCC ATCTTCAATG TGCTATTGGT GTGTCTAATA 
TTTTGGCTAA TTTTTGCCAT AATGGGTGTA CAGCTTTTTG CTGGAAAATA TTTTAAGTGC 
GAGGACATGA ATGGCACGAA GCTCAGCCAC GAGATCATAC CAAATCGCAA TGCCTGCGAG 
AGCGAGAACT ACACGTGGGT GAATTCAGCA ATGAATTTCG ATCATGTAGG TAACGCGTAT 
CTGTGCCTTT TCCAAGTGGC CACCTTCAAA GGCTGGATAC AAATCATGAA CGATGCTATC 
GATTCACGAG AGGTGGACAA GCAACCAATT CGTGAAACGA ACATCTACAT GTATTTATAT 
TTCGTATTCT TCATCATATT TGGATCATTT TTCACACTCA ATCTGTTCAT TGGTGTTATC 
ATTGATAATT TTAATGAGCA AAAGAAAAAA GCAGGTGGAT CATTAGAAAT GTTCATGACA 
GAAGATCAGA AAAAGTACTA TAATGCTATG AAAAAGATGG GCTCTAAAAA ACCATTAAAA 
GCCATTCCAA GACCAAGGTG GCGACCACAA GCAATAGTCT TTGAAATAGT AACCGATAAG 
AAATTCGATA TAATCATTAT GTTATTCATT GGTCTGAACA TGTTCACCAT GACCCTCGAT 
CGTTACGATG CGTCGGACAC GTATAACGCG GTCCTAGACT ATCTCAATGC GATATTCGTA 
GTTATTTTCA GTTCCGAATG TCTATTAAAA ATATTCGCTT TACGATATCA CTATTTTATT 
GAGCCATGGA ATTTATTTGA TGTAGTAGTT GTCATTTTAT CCATCTTAGG TCTTGTACTT 
AGCGATATTA TCGAGAAGTA CTTCGTGTCG CCGACCCTGC TCCGAGTGGT GCGTGTGGCG 
AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG GGAGCCAAGG GCATTCGGAC ACTGCTCTTC 
GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC AACATCTGCC TGCTGCTGTT CCTGGTCATG 
TTCATCTTTG CCATTTTCGG CATGTCGTTC TTCATGCACG TGAAGGAGAA GAGCGGCATC 
AACGACGTCT ACAACTTCAA GACCTTTGGC CAGAGCATGA TCCTGCTCTT TCAGATGTCG 
ACGTCAGCCG GTTGGGATGG TGTACTGGAC GCCATTATCA ATGAGGAAGC ATGCGATCCA 
CCCGACAACG ACAAAGGCTA TCCGGGCAAT TGTGGTTCAG CGACCGTTGG AATAACGTTT 
CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG ATAGTTATTA ATATGTACAT TGCTGTCATT 
CTCGAGAACT ATAGTCAGGC CACCGAGGAC GTGCAAGAGG GTCTAACCGA CGACGACTAC 
GACATGTACT ATGAGATCTG GCAGCAATTC GATCCGGAGG GCACCCAGTA CATACGCTAT 
GATCAGCTGT CCGAATTCCT GGACGTACTG GAGCCCCCGC TGCAGATCCA CAAACCGAAC 
AAGTACAAGA TCATATCGAT GGACATACCC ATCTGTCGCG GTGACCTCAT GTACTGCGTC 
GACATCCTCG ACGCCCTTAC GAAAGACTTC TTTGCGCGGA AGGGCAATCC GATAGAGGAG 
ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC CCGGATACGG AGGGCTACGA GCCCGTCTCA 
TCAACGCTGT GGCGTCAGCG TGAGGAGTAC TGCGCCCGGC TAATCCAGCA CGCCTGGCGA 
AAGCACAAGG CGCGCGGCGA GGGAGGTGGG TCCTTTGAGC CGGATACGGA TCATGGCGAT 
GGCGGTGATC CGGATGCCGG GGACCCGGCG CCCGATGAAG CAACGGACGG CGATGCGCCC 
GCTGGTGGAG ATGGTAGTGT TAACGGTACT GCAGAAGGAG CTGCCGATGC CGATGAGAGT 
AATGTAAATA GTCCGGGTGA GGATGCAGCG GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG 
GCGGCGGGCA CGACGACGGC GGGAAGTCCC GGAGCGGGTA GCGCCGGGCG ACAGACCGCC 
GTTCTCGTGG AGAGCGACGG GTTCGTGACG AAGAACGGCC ACAAGGTGGT CATCCACTCG 
CGATCGCCGA GCATCACGTC GCGCACGGCG GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201717.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188646.1 Drosophila melanogaster paralytic (para), transcript variant AY, mRNA.

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CloneID	OFa03663
Clone ID Related Accession (Same CDS sequence)	NM_001201717.1
Accession Version	NM_001201717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AY
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGCAAAGGC GTTTGTCGTT GTATATCTGC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGATGATT 
GGCAACTCAA TTAACCACCA AGACAATAGA CTGGAACACG AGCTAAACCA TAGAGGTTTG 
TCCTTACAGG ACGACGACAC TGCCAGCATT AACTCATATG GTAGCCATAA GAATCGACCA 
TTCAAGGACG AGAGCCACAA GGGCAGCGCC GAGACGATGG AGGGCGAGGA GAAGCGCGAC 
GCCAGCAAGG AGGATTTAGG TCTCGACGAG GAACTGGACG AGGAGGGCGA ATGCGAGGAG 
GGCCCGCTCG ACGGTGATAT CATTATTCAT GCACACGACG AGGATATACT CGATGAATAT 
CCAGCTGATT GCTGCCCCGA TTCGTACTAT AAGAAATTTC CGATCTTAGC CGGTGACGAT 
GACTCGCCGT TCTGGCAAGG ATGGGGCAAT TTACGACTGA AAACTTTTCA ATTAATTGAA 
AATAAATATT TTGAAACAGC TGTTATCACT ATGATTTTAA TGAGTAGCTT AGCTTTGGCA 
TTAGAAGATG TACATCTGCC ACAAAGACCC ATACTGCAGG ATATTTTATA CTATATGGAC 
AGAATATTTA CGGTTATATT CTTCTTGGAA ATGTTAATCA AGTGGTTGGC GCTCGGCTTC 
AAAGTGTACT TCACCAACGC GTGGTGTTGG CTCGATTTCG TGATTGTCAT GCTGTCGCTA 
ATTAATTTGG CCGCGGTCTG GTCCGGGGCC GATGATGTGC CCGCCTTTCG CTCGATGCGA 
ACTCTGCGCG CCCTGCGACC ACTGCGGGCA GTTTCGCGCT GGGAGGGCAT GAAAGTCGTC 
GTTAATGCGC TGGTACAAGC TATACCGTCC ATCTTCAATG TGCTATTGGT GTGTCTAATA 
TTTTGGCTAA TTTTTGCCAT AATGGGTGTA CAGCTTTTTG CTGGAAAATA TTTTAAGTGC 
GAGGACATGA ATGGCACGAA GCTCAGCCAC GAGATCATAC CAAATCGCAA TGCCTGCGAG 
AGCGAGAACT ACACGTGGGT GAATTCAGCA ATGAATTTCG ATCATGTAGG TAACGCGTAT 
CTGTGCCTTT TCCAAGTGGC CACCTTCAAA GGCTGGATAC AAATCATGAA CGATGCTATC 
GATTCACGAG AGGTGGACAA GCAACCAATT CGTGAAACGA ACATCTACAT GTATTTATAT 
TTCGTATTCT TCATCATATT TGGATCATTT TTCACACTCA ATCTGTTCAT TGGTGTTATC 
ATTGATAATT TTAATGAGCA AAAGAAAAAA GCAGGTGGAT CATTAGAAAT GTTCATGACA 
GAAGATCAGA AAAAGTACTA TAATGCTATG AAAAAGATGG GCTCTAAAAA ACCATTAAAA 
GCCATTCCAA GACCAAGGTG GCGACCACAA GCAATAGTCT TTGAAATAGT AACCGATAAG 
AAATTCGATA TAATCATTAT GTTATTCATT GGTCTGAACA TGTTCACCAT GACCCTCGAT 
CGTTACGATG CGTCGGACAC GTATAACGCG GTCCTAGACT ATCTCAATGC GATATTCGTA 
GTTATTTTCA GTTCCGAATG TCTATTAAAA ATATTCGCTT TACGATATCA CTATTTTATT 
GAGCCATGGA ATTTATTTGA TGTAGTAGTT GTCATTTTAT CCATCTTAGG TCTTGTACTT 
AGCGATATTA TCGAGAAGTA CTTCGTGTCG CCGACCCTGC TCCGAGTGGT GCGTGTGGCG 
AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG GGAGCCAAGG GCATTCGGAC ACTGCTCTTC 
GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC AACATCTGCC TGCTGCTGTT CCTGGTCATG 
TTCATCTTTG CCATTTTCGG CATGTCGTTC TTCATGCACG TGAAGGAGAA GAGCGGCATC 
AACGACGTCT ACAACTTCAA GACCTTTGGC CAGAGCATGA TCCTGCTCTT TCAGATGTCG 
ACGTCAGCCG GTTGGGATGG TGTACTGGAC GCCATTATCA ATGAGGAAGC ATGCGATCCA 
CCCGACAACG ACAAAGGCTA TCCGGGCAAT TGTGGTTCAG CGACCGTTGG AATAACGTTT 
CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG ATAGTTATTA ATATGTACAT TGCTGTCATT 
CTCGAGAACT ATAGTCAGGC CACCGAGGAC GTGCAAGAGG GTCTAACCGA CGACGACTAC 
GACATGTACT ATGAGATCTG GCAGCAATTC GATCCGGAGG GCACCCAGTA CATACGCTAT 
GATCAGCTGT CCGAATTCCT GGACGTACTG GAGCCCCCGC TGCAGATCCA CAAACCGAAC 
AAGTACAAGA TCATATCGAT GGACATACCC ATCTGTCGCG GTGACCTCAT GTACTGCGTC 
GACATCCTCG ACGCCCTTAC GAAAGACTTC TTTGCGCGGA AGGGCAATCC GATAGAGGAG 
ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC CCGGATACGG AGGGCTACGA GCCCGTCTCA 
TCAACGCTGT GGCGTCAGCG TGAGGAGTAC TGCGCCCGGC TAATCCAGCA CGCCTGGCGA 
AAGCACAAGG CGCGCGGCGA GGGAGGTGGG TCCTTTGAGC CGGATACGGA TCATGGCGAT 
GGCGGTGATC CGGATGCCGG GGACCCGGCG CCCGATGAAG CAACGGACGG CGATGCGCCC 
GCTGGTGGAG ATGGTAGTGT TAACGGTACT GCAGAAGGAG CTGCCGATGC CGATGAGAGT 
AATGTAAATA GTCCGGGTGA GGATGCAGCG GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG 
GCGGCGGGCA CGACGACGGC GGGAAGTCCC GGAGCGGGTA GCGCCGGGCG ACAGACCGCC 
GTTCTCGTGG AGAGCGACGG GTTCGTGACG AAGAACGGCC ACAAGGTGGT CATCCACTCG 
CGATCGCCGA GCATCACGTC GCGCACGGCG GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188646.1
CDS	269..6667
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATMIGNSINHQDNRLEHELNHRGLSLQDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201717.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AY, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201717.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGCAAAGGC GTTTGTCGTT GTATATCTGC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGATGATT 
GGCAACTCAA TTAACCACCA AGACAATAGA CTGGAACACG AGCTAAACCA TAGAGGTTTG 
TCCTTACAGG ACGACGACAC TGCCAGCATT AACTCATATG GTAGCCATAA GAATCGACCA 
TTCAAGGACG AGAGCCACAA GGGCAGCGCC GAGACGATGG AGGGCGAGGA GAAGCGCGAC 
GCCAGCAAGG AGGATTTAGG TCTCGACGAG GAACTGGACG AGGAGGGCGA ATGCGAGGAG 
GGCCCGCTCG ACGGTGATAT CATTATTCAT GCACACGACG AGGATATACT CGATGAATAT 
CCAGCTGATT GCTGCCCCGA TTCGTACTAT AAGAAATTTC CGATCTTAGC CGGTGACGAT 
GACTCGCCGT TCTGGCAAGG ATGGGGCAAT TTACGACTGA AAACTTTTCA ATTAATTGAA 
AATAAATATT TTGAAACAGC TGTTATCACT ATGATTTTAA TGAGTAGCTT AGCTTTGGCA 
TTAGAAGATG TACATCTGCC ACAAAGACCC ATACTGCAGG ATATTTTATA CTATATGGAC 
AGAATATTTA CGGTTATATT CTTCTTGGAA ATGTTAATCA AGTGGTTGGC GCTCGGCTTC 
AAAGTGTACT TCACCAACGC GTGGTGTTGG CTCGATTTCG TGATTGTCAT GCTGTCGCTA 
ATTAATTTGG CCGCGGTCTG GTCCGGGGCC GATGATGTGC CCGCCTTTCG CTCGATGCGA 
ACTCTGCGCG CCCTGCGACC ACTGCGGGCA GTTTCGCGCT GGGAGGGCAT GAAAGTCGTC 
GTTAATGCGC TGGTACAAGC TATACCGTCC ATCTTCAATG TGCTATTGGT GTGTCTAATA 
TTTTGGCTAA TTTTTGCCAT AATGGGTGTA CAGCTTTTTG CTGGAAAATA TTTTAAGTGC 
GAGGACATGA ATGGCACGAA GCTCAGCCAC GAGATCATAC CAAATCGCAA TGCCTGCGAG 
AGCGAGAACT ACACGTGGGT GAATTCAGCA ATGAATTTCG ATCATGTAGG TAACGCGTAT 
CTGTGCCTTT TCCAAGTGGC CACCTTCAAA GGCTGGATAC AAATCATGAA CGATGCTATC 
GATTCACGAG AGGTGGACAA GCAACCAATT CGTGAAACGA ACATCTACAT GTATTTATAT 
TTCGTATTCT TCATCATATT TGGATCATTT TTCACACTCA ATCTGTTCAT TGGTGTTATC 
ATTGATAATT TTAATGAGCA AAAGAAAAAA GCAGGTGGAT CATTAGAAAT GTTCATGACA 
GAAGATCAGA AAAAGTACTA TAATGCTATG AAAAAGATGG GCTCTAAAAA ACCATTAAAA 
GCCATTCCAA GACCAAGGTG GCGACCACAA GCAATAGTCT TTGAAATAGT AACCGATAAG 
AAATTCGATA TAATCATTAT GTTATTCATT GGTCTGAACA TGTTCACCAT GACCCTCGAT 
CGTTACGATG CGTCGGACAC GTATAACGCG GTCCTAGACT ATCTCAATGC GATATTCGTA 
GTTATTTTCA GTTCCGAATG TCTATTAAAA ATATTCGCTT TACGATATCA CTATTTTATT 
GAGCCATGGA ATTTATTTGA TGTAGTAGTT GTCATTTTAT CCATCTTAGG TCTTGTACTT 
AGCGATATTA TCGAGAAGTA CTTCGTGTCG CCGACCCTGC TCCGAGTGGT GCGTGTGGCG 
AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG GGAGCCAAGG GCATTCGGAC ACTGCTCTTC 
GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC AACATCTGCC TGCTGCTGTT CCTGGTCATG 
TTCATCTTTG CCATTTTCGG CATGTCGTTC TTCATGCACG TGAAGGAGAA GAGCGGCATC 
AACGACGTCT ACAACTTCAA GACCTTTGGC CAGAGCATGA TCCTGCTCTT TCAGATGTCG 
ACGTCAGCCG GTTGGGATGG TGTACTGGAC GCCATTATCA ATGAGGAAGC ATGCGATCCA 
CCCGACAACG ACAAAGGCTA TCCGGGCAAT TGTGGTTCAG CGACCGTTGG AATAACGTTT 
CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG ATAGTTATTA ATATGTACAT TGCTGTCATT 
CTCGAGAACT ATAGTCAGGC CACCGAGGAC GTGCAAGAGG GTCTAACCGA CGACGACTAC 
GACATGTACT ATGAGATCTG GCAGCAATTC GATCCGGAGG GCACCCAGTA CATACGCTAT 
GATCAGCTGT CCGAATTCCT GGACGTACTG GAGCCCCCGC TGCAGATCCA CAAACCGAAC 
AAGTACAAGA TCATATCGAT GGACATACCC ATCTGTCGCG GTGACCTCAT GTACTGCGTC 
GACATCCTCG ACGCCCTTAC GAAAGACTTC TTTGCGCGGA AGGGCAATCC GATAGAGGAG 
ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC CCGGATACGG AGGGCTACGA GCCCGTCTCA 
TCAACGCTGT GGCGTCAGCG TGAGGAGTAC TGCGCCCGGC TAATCCAGCA CGCCTGGCGA 
AAGCACAAGG CGCGCGGCGA GGGAGGTGGG TCCTTTGAGC CGGATACGGA TCATGGCGAT 
GGCGGTGATC CGGATGCCGG GGACCCGGCG CCCGATGAAG CAACGGACGG CGATGCGCCC 
GCTGGTGGAG ATGGTAGTGT TAACGGTACT GCAGAAGGAG CTGCCGATGC CGATGAGAGT 
AATGTAAATA GTCCGGGTGA GGATGCAGCG GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG 
GCGGCGGGCA CGACGACGGC GGGAAGTCCC GGAGCGGGTA GCGCCGGGCG ACAGACCGCC 
GTTCTCGTGG AGAGCGACGG GTTCGTGACG AAGAACGGCC ACAAGGTGGT CATCCACTCG 
CGATCGCCGA GCATCACGTC GCGCACGGCG GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201698.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188627.1 Drosophila melanogaster paralytic (para), transcript variant AF, mRNA.

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CloneID	OFa03664
Clone ID Related Accession (Same CDS sequence)	NM_001201698.1
Accession Version	NM_001201698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AF
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGTGAGAGG ACCAACCAGA TCAGTTGGAT TTGGAGCGAA 
GAGCATGGTG ACAACGAACT GGAGCTGGGC CACGACGAGA TCCTCGCCGA CGGCCTCATC 
AAGAAGGGGA TCAAGGAGCA GACGCAACTG GAGGTGGCCA TCGGGGATGG CATGGAATTC 
ACGATACACG GCGACATGAA GAACAACAAG CCGAAGAAAT CCAAATATCT AAATAACGCA 
ACGGACGACG ACACTGCCAG CATTAACTCA TATGGTAGCC ATAAGAATCG ACCATTCAAG 
GACGAGAGCC ACAAGGGCAG CGCCGAGACG ATGGAGGGCG AGGAGAAGCG CGACGCCAGC 
AAGGAGGATT TAGGTCTCGA CGAGGAACTG GACGAGGAGG GCGAATGCGA GGAGGGCCCG 
CTCGACGGTG ATATCATTAT TCATGCACAC GACGAGGATA TACTCGATGA ATATCCAGCT 
GATTGCTGCC CCGATTCGTA CTATAAGAAA TTTCCGATCT TAGCCGGTGA CGATGACTCG 
CCGTTCTGGC AAGGATGGGG CAATTTACGA CTGAAAACTT TTCAATTAAT TGAAAATAAA 
TATTTTGAAA CAGCTGTTAT CACTATGATT TTAATGAGTA GCTTAGCTTT GGCATTAGAA 
GATGTACATC TGCCACAAAG ACCCATACTG CAGGATATTT TATACTATAT GGACAGAATA 
TTTACGGTTA TATTCTTCTT GGAAATGTTA ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG 
TACTTCACCA ACGCGTGGTG TTGGCTCGAT TTCGTGATTG TCATGGTATC GCTTATCAAC 
TTCGTTGCTT CACTTGTTGG AGCTGGTGGT ATTCAAGCCT TCAAGACTAT GCGAACGTTA 
AGAGCACTGA GACCACTACG TGCCATGTCC CGTATGCAGG GCATGAGGGT CGTCGTTAAT 
GCGCTGGTAC AAGCTATACC GTCCATCTTC AATGTGCTAT TGGTGTGTCT AATATTTTGG 
CTAATTTTTG CCATAATGGG TGTACAGCTT TTTGCTGGAA AATATTTTAA GTGCGAGGAC 
ATGAATGGCA CGAAGCTCAG CCACGAGATC ATACCAAATC GCAATGCCTG CGAGAGCGAG 
AACTACACGT GGGTGAATTC AGCAATGAAT TTCGATCATG TAGGTAACGC GTATCTGTGC 
CTTTTCCAAG TGGCCACCTT CAAAGGCTGG ATACAAATCA TGAACGATGC TATCGATTCA 
CGAGAGGTGG ACAAGCAACC AATTCGTGAA ACGAACATCT ACATGTATTT ATATTTCGTA 
TTCTTCATCA TATTTGGATC ATTTTTCACA CTCAATCTGT TCATTGGTGT TATCATTGAT 
AATTTTAATG AGCAAAAGAA AAAAGCAGGT GGATCATTAG AAATGTTCAT GACAGAAGAT 
CAGAAAAAGT ACTATAATGC TATGAAAAAG ATGGGCTCTA AAAAACCATT AAAAGCCATT 
CCAAGACCAA GGTGGCGACC ACAAGCAATA GTCTTTGAAA TAGTAACCGA TAAGAAATTC 
GATATAATCA TTATGTTATT CATTGGTCTG AACATGTTCA CCATGACCCT CGATCGTTAC 
GATGCGTCGG ACACGTATAA CGCGGTCCTA GACTATCTCA ATGCGATATT CGTAGTTATT 
TTCAGTTCCG AATGTCTATT AAAAATATTC GCTTTACGAT ATCACTATTT TATTGAGCCA 
TGGAATTTAT TTGATGTAGT AGTTGTCATT TTATCCATCT TAGGTCTTGT ACTTAGCGAT 
ATTATCGAGA AGTACTTCGT GTCGCCGACC CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG 
GGCCGTGTCC TTCGACTGGT GAAGGGAGCC AAGGGCATTC GGACACTGCT CTTCGCGTTG 
GCCATGTCGC TGCCGGCCCT GTTCAACATC TGCCTGCTGC TGTTCCTGGT CATGTTCATC 
TTTGCCATTT TCGGCATGTC GTTCTTCATG CACGTGAAGG AGAAGAGCGG CATCAACGAC 
GTCTACAACT TCAAGACCTT TGGCCAGAGC ATGATCCTGC TCTTTCAGAT GTCGACGTCA 
GCCGGTTGGG ATGGTGTACT GGACGCCATT ATCAATGAGG AAGCATGCGA TCCACCCGAC 
AACGACAAAG GCTATCCGGG CAATTGTGGT TCAGCGACCG TTGGAATAAC GTTTCTCCTC 
TCATACCTAG TTATAAGCTT TTTGATAGTT ATTAATATGT ACATTGCTGT CATTCTCGAG 
AACTATAGTC AGGCCACCGA GGACGTGCAA GAGGGTCTAA CCGACGACGA CTACGACATG 
TACTATGAGA TCTGGCAGCA ATTCGATCCG GAGGGCACCC AGTACATACG CTATGATCAG 
CTGTCCGAAT TCCTGGACGT ACTGGAGCCC CCGCTGCAGA TCCACAAACC GAACAAGTAC 
AAGATCATAT CGATGGACAT ACCCATCTGT CGCGGTGACC TCATGTACTG CGTCGACATC 
CTCGACGCCC TTACGAAAGA CTTCTTTGCG CGGAAGGGCA ATCCGATAGA GGAGACGGGT 
GAGATTGGTG AGATAGCGGC CCGCCCGGAT ACGGAGGGCT ACGAGCCCGT CTCATCAACG 
CTGTGGCGTC AGCGTGAGGA GTACTGCGCC CGGCTAATCC AGCACGCCTG GCGAAAGCAC 
AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT GAGCCGGATA CGGATCATGG CGATGGCGGT 
GATCCGGATG CCGGGGACCC GGCGCCCGAT GAAGCAACGG ACGGCGATGC GCCCGCTGGT 
GGAGATGGTA GTGTTAACGG TACTGCAGAA GGAGCTGCCG ATGCCGATGA GAGTAATGTA 
AATAGTCCGG GTGAGGATGC AGCGGCGGCG GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG 
GGCACGACGA CGGCGGGAAG TCCCGGAGCG GGTAGCGCCG GGCGACAGAC CGCCGTTCTC 
GTGGAGAGCG ACGGGTTCGT GACGAAGAAC GGCCACAAGG TGGTCATCCA CTCGCGATCG 
CCGAGCATCA CGTCGCGCAC GGCGGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188627.1
CDS	269..6601
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201698.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AF, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201698.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTATTCAA ACTGGCCAAG TCTTGGCCCA CACTTAATTT ACTCATTTCG 
ATTATGGGAC GCACCATGGG CGCTTTGGGT AATCTGACAT TTGTACTTTG CATTATCATC 
TTCATCTTTG CGGTGATGGG AATGCAACTG TTCGGAAAGA ATTATCATGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGTGAGAGG ACCAACCAGA TCAGTTGGAT TTGGAGCGAA 
GAGCATGGTG ACAACGAACT GGAGCTGGGC CACGACGAGA TCCTCGCCGA CGGCCTCATC 
AAGAAGGGGA TCAAGGAGCA GACGCAACTG GAGGTGGCCA TCGGGGATGG CATGGAATTC 
ACGATACACG GCGACATGAA GAACAACAAG CCGAAGAAAT CCAAATATCT AAATAACGCA 
ACGGACGACG ACACTGCCAG CATTAACTCA TATGGTAGCC ATAAGAATCG ACCATTCAAG 
GACGAGAGCC ACAAGGGCAG CGCCGAGACG ATGGAGGGCG AGGAGAAGCG CGACGCCAGC 
AAGGAGGATT TAGGTCTCGA CGAGGAACTG GACGAGGAGG GCGAATGCGA GGAGGGCCCG 
CTCGACGGTG ATATCATTAT TCATGCACAC GACGAGGATA TACTCGATGA ATATCCAGCT 
GATTGCTGCC CCGATTCGTA CTATAAGAAA TTTCCGATCT TAGCCGGTGA CGATGACTCG 
CCGTTCTGGC AAGGATGGGG CAATTTACGA CTGAAAACTT TTCAATTAAT TGAAAATAAA 
TATTTTGAAA CAGCTGTTAT CACTATGATT TTAATGAGTA GCTTAGCTTT GGCATTAGAA 
GATGTACATC TGCCACAAAG ACCCATACTG CAGGATATTT TATACTATAT GGACAGAATA 
TTTACGGTTA TATTCTTCTT GGAAATGTTA ATCAAGTGGT TGGCGCTCGG CTTCAAAGTG 
TACTTCACCA ACGCGTGGTG TTGGCTCGAT TTCGTGATTG TCATGGTATC GCTTATCAAC 
TTCGTTGCTT CACTTGTTGG AGCTGGTGGT ATTCAAGCCT TCAAGACTAT GCGAACGTTA 
AGAGCACTGA GACCACTACG TGCCATGTCC CGTATGCAGG GCATGAGGGT CGTCGTTAAT 
GCGCTGGTAC AAGCTATACC GTCCATCTTC AATGTGCTAT TGGTGTGTCT AATATTTTGG 
CTAATTTTTG CCATAATGGG TGTACAGCTT TTTGCTGGAA AATATTTTAA GTGCGAGGAC 
ATGAATGGCA CGAAGCTCAG CCACGAGATC ATACCAAATC GCAATGCCTG CGAGAGCGAG 
AACTACACGT GGGTGAATTC AGCAATGAAT TTCGATCATG TAGGTAACGC GTATCTGTGC 
CTTTTCCAAG TGGCCACCTT CAAAGGCTGG ATACAAATCA TGAACGATGC TATCGATTCA 
CGAGAGGTGG ACAAGCAACC AATTCGTGAA ACGAACATCT ACATGTATTT ATATTTCGTA 
TTCTTCATCA TATTTGGATC ATTTTTCACA CTCAATCTGT TCATTGGTGT TATCATTGAT 
AATTTTAATG AGCAAAAGAA AAAAGCAGGT GGATCATTAG AAATGTTCAT GACAGAAGAT 
CAGAAAAAGT ACTATAATGC TATGAAAAAG ATGGGCTCTA AAAAACCATT AAAAGCCATT 
CCAAGACCAA GGTGGCGACC ACAAGCAATA GTCTTTGAAA TAGTAACCGA TAAGAAATTC 
GATATAATCA TTATGTTATT CATTGGTCTG AACATGTTCA CCATGACCCT CGATCGTTAC 
GATGCGTCGG ACACGTATAA CGCGGTCCTA GACTATCTCA ATGCGATATT CGTAGTTATT 
TTCAGTTCCG AATGTCTATT AAAAATATTC GCTTTACGAT ATCACTATTT TATTGAGCCA 
TGGAATTTAT TTGATGTAGT AGTTGTCATT TTATCCATCT TAGGTCTTGT ACTTAGCGAT 
ATTATCGAGA AGTACTTCGT GTCGCCGACC CTGCTCCGAG TGGTGCGTGT GGCGAAAGTG 
GGCCGTGTCC TTCGACTGGT GAAGGGAGCC AAGGGCATTC GGACACTGCT CTTCGCGTTG 
GCCATGTCGC TGCCGGCCCT GTTCAACATC TGCCTGCTGC TGTTCCTGGT CATGTTCATC 
TTTGCCATTT TCGGCATGTC GTTCTTCATG CACGTGAAGG AGAAGAGCGG CATCAACGAC 
GTCTACAACT TCAAGACCTT TGGCCAGAGC ATGATCCTGC TCTTTCAGAT GTCGACGTCA 
GCCGGTTGGG ATGGTGTACT GGACGCCATT ATCAATGAGG AAGCATGCGA TCCACCCGAC 
AACGACAAAG GCTATCCGGG CAATTGTGGT TCAGCGACCG TTGGAATAAC GTTTCTCCTC 
TCATACCTAG TTATAAGCTT TTTGATAGTT ATTAATATGT ACATTGCTGT CATTCTCGAG 
AACTATAGTC AGGCCACCGA GGACGTGCAA GAGGGTCTAA CCGACGACGA CTACGACATG 
TACTATGAGA TCTGGCAGCA ATTCGATCCG GAGGGCACCC AGTACATACG CTATGATCAG 
CTGTCCGAAT TCCTGGACGT ACTGGAGCCC CCGCTGCAGA TCCACAAACC GAACAAGTAC 
AAGATCATAT CGATGGACAT ACCCATCTGT CGCGGTGACC TCATGTACTG CGTCGACATC 
CTCGACGCCC TTACGAAAGA CTTCTTTGCG CGGAAGGGCA ATCCGATAGA GGAGACGGGT 
GAGATTGGTG AGATAGCGGC CCGCCCGGAT ACGGAGGGCT ACGAGCCCGT CTCATCAACG 
CTGTGGCGTC AGCGTGAGGA GTACTGCGCC CGGCTAATCC AGCACGCCTG GCGAAAGCAC 
AAGGCGCGCG GCGAGGGAGG TGGGTCCTTT GAGCCGGATA CGGATCATGG CGATGGCGGT 
GATCCGGATG CCGGGGACCC GGCGCCCGAT GAAGCAACGG ACGGCGATGC GCCCGCTGGT 
GGAGATGGTA GTGTTAACGG TACTGCAGAA GGAGCTGCCG ATGCCGATGA GAGTAATGTA 
AATAGTCCGG GTGAGGATGC AGCGGCGGCG GCAGCAGCAG CAGCAGCAGC GGCGGCGGCG 
GGCACGACGA CGGCGGGAAG TCCCGGAGCG GGTAGCGCCG GGCGACAGAC CGCCGTTCTC 
GTGGAGAGCG ACGGGTTCGT GACGAAGAAC GGCCACAAGG TGGTCATCCA CTCGCGATCG 
CCGAGCATCA CGTCGCGCAC GGCGGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201719.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188648.1 Drosophila melanogaster paralytic (para), transcript variant BA, mRNA.

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CloneID	OFa03665
Clone ID Related Accession (Same CDS sequence)	NM_001201719.1
Accession Version	NM_001201719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform BA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTTTTCAA GCTGGCCAAA TCGTGGCCGA CGCTGAATTT GCTCATATCG 
ATTATGGGCC GCACTGTCGG CGCCCTCGGC AATCTGACCT TCGTCCTGTG CATTATTATC 
TTCATATTCG CGGTCATGGG CATGCAGCTG TTTGGCAAGA ACTACACAGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGCAAAGGC GTTTGTCGTT GTATATCTGC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGATGATT 
GGCAACTCAA TTAACCACCA AGACAATAGA CTGGAACACG AGCTAAACCA TAGAGGTTTG 
TCCTTACAGG ACGACGACAC TGCCAGCATT AACTCATATG GTAGCCATAA GAATCGACCA 
TTCAAGGACG AGAGCCACAA GGGCAGCGCC GAGACGATGG AGGGCGAGGA GAAGCGCGAC 
GCCAGCAAGG AGGATTTAGG TCTCGACGAG GAACTGGACG AGGAGGGCGA ATGCGAGGAG 
GGCCCGCTCG ACGGTGATAT CATTATTCAT GCACACGACG AGGATATACT CGATGAATAT 
CCAGCTGATT GCTGCCCCGA TTCGTACTAT AAGAAATTTC CGATCTTAGC CGGTGACGAT 
GACTCGCCGT TCTGGCAAGG ATGGGGCAAT TTACGACTGA AAACTTTTCA ATTAATTGAA 
AATAAATATT TTGAAACAGC TGTTATCACT ATGATTTTAA TGAGTAGCTT AGCTTTGGCA 
TTAGAAGATG TACATCTGCC ACAAAGACCC ATACTGCAGG ATATTTTATA CTATATGGAC 
AGAATATTTA CGGTTATATT CTTCTTGGAA ATGTTAATCA AGTGGTTGGC GCTCGGCTTC 
AAAGTGTACT TCACCAACGC GTGGTGTTGG CTCGATTTCG TGATTGTCAT GGTATCGCTT 
ATCAACTTCG TTGCTTCACT TGTTGGAGCT GGTGGTATTC AAGCCTTCAA GACTATGCGA 
ACGTTAAGAG CACTGAGACC ACTACGTGCC ATGTCCCGTA TGCAGGGCAT GAGGGTCGTC 
GTTAATGCGC TGGTACAAGC TATACCGTCC ATCTTCAATG TGCTATTGGT GTGTCTAATA 
TTTTGGCTAA TTTTTGCCAT AATGGGTGTA CAGCTTTTTG CTGGAAAATA TTTTAAGTGC 
GAGGACATGA ATGGCACGAA GCTCAGCCAC GAGATCATAC CAAATCGCAA TGCCTGCGAG 
AGCGAGAACT ACACGTGGGT GAATTCAGCA ATGAATTTCG ATCATGTAGG TAACGCGTAT 
CTGTGCCTTT TCCAAGTGGC CACCTTCAAA GGCTGGATAC AAATCATGAA CGATGCTATC 
GATTCACGAG AGGTGGACAA GCAACCAATT CGTGAAACGA ACATCTACAT GTATTTATAT 
TTCGTATTCT TCATCATATT TGGATCATTT TTCACACTCA ATCTGTTCAT TGGTGTTATC 
ATTGATAATT TTAATGAGCA AAAGAAAAAA GCAGGTGGAT CATTAGAAAT GTTCATGACA 
GAAGATCAGA AAAAGTACTA TAATGCTATG AAAAAGATGG GCTCTAAAAA ACCATTAAAA 
GCCATTCCAA GACCAAGGTG GCGACCACAA GCAATAGTCT TTGAAATAGT AACCGATAAG 
AAATTCGATA TAATCATTAT GTTATTCATT GGTCTGAACA TGTTCACCAT GACCCTCGAT 
CGTTACGATG CGTCGGACAC GTATAACGCG GTCCTAGACT ATCTCAATGC GATATTCGTA 
GTTATTTTCA GTTCCGAATG TCTATTAAAA ATATTCGCTT TACGATATCA CTATTTTATT 
GAGCCATGGA ATTTATTTGA TGTAGTAGTT GTCATTTTAT CCATCTTAGG TCTTGTACTT 
AGCGATATTA TCGAGAAGTA CTTCGTGTCG CCGACCCTGC TCCGAGTGGT GCGTGTGGCG 
AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG GGAGCCAAGG GCATTCGGAC ACTGCTCTTC 
GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC AACATCTGCC TGCTGCTGTT CCTGGTCATG 
TTCATCTTTG CCATTTTCGG CATGTCGTTC TTCATGCACG TGAAGGAGAA GAGCGGCATC 
AACGACGTCT ACAACTTCAA GACCTTTGGC CAGAGCATGA TCCTGCTCTT TCAGATGTCG 
ACGTCAGCCG GTTGGGATGG TGTACTGGAC GCCATTATCA ATGAGGAAGC ATGCGATCCA 
CCCGACAACG ACAAAGGCTA TCCGGGCAAT TGTGGTTCAG CGACCGTTGG AATAACGTTT 
CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG ATAGTTATTA ATATGTACAT TGCTGTCATT 
CTCGAGAACT ATAGTCAGGC CACCGAGGAC GTGCAAGAGG GTCTAACCGA CGACGACTAC 
GACATGTACT ATGAGATCTG GCAGCAATTC GATCCGGAGG GCACCCAGTA CATACGCTAT 
GATCAGCTGT CCGAATTCCT GGACGTACTG GAGCCCCCGC TGCAGATCCA CAAACCGAAC 
AAGTACAAGA TCATATCGAT GGACATACCC ATCTGTCGCG GTGACCTCAT GTACTGCGTC 
GACATCCTCG ACGCCCTTAC GAAAGACTTC TTTGCGCGGA AGGGCAATCC GATAGAGGAG 
ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC CCGGATACGG AGGGCTACGA GCCCGTCTCA 
TCAACGCTGT GGCGTCAGCG TGAGGAGTAC TGCGCCCGGC TAATCCAGCA CGCCTGGCGA 
AAGCACAAGG CGCGCGGCGA GGGAGGTGGG TCCTTTGAGC CGGATACGGA TCATGGCGAT 
GGCGGTGATC CGGATGCCGG GGACCCGGCG CCCGATGAAG CAACGGACGG CGATGCGCCC 
GCTGGTGGAG ATGGTAGTGT TAACGGTACT GCAGAAGGAG CTGCCGATGC CGATGAGAGT 
AATGTAAATA GTCCGGGTGA GGATGCAGCG GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG 
GCGGCGGGCA CGACGACGGC GGGAAGTCCC GGAGCGGGTA GCGCCGGGCG ACAGACCGCC 
GTTCTCGTGG AGAGCGACGG GTTCGTGACG AAGAACGGCC ACAAGGTGGT CATCCACTCG 
CGATCGCCGA GCATCACGTC GCGCACGGCG GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188648.1
CDS	269..6667
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATMIGNSINHQDNRLEHELNHRGLSLQDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201719.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant BA, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201719.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG ATCCGATATG ATGACGAGGA CGAGGATGAA 
GGTCCACAAC CGGATCCTAC ACTTGAACAG GGTGTGCCAA TACCTGTTCG ATTGCAGGGC 
AGCTTCCCGC CGGAATTGGC CTCCACTCCT CTCGAGGATA TCGATCCCTA CTACAGCAAT 
GTACTGACAT TCGTAGTTGT AAGCAAAGGA AAAGATATTT TTCGCTTTTC TGCATCAAAA 
GCAATGTGGA TGCTCGATCC ATTCAATCCG ATACGTCGTG TGGCCATTTA CATTCTAGTG 
CATCCATTAT TTTCCCTATT CATCATCACC ACAATTCTCG TCAACTGCAT CCTGATGATA 
ATGCCGACAA CGCCCACGGT TGAGTCCACT GAGGTGATAT TCACCGGAAT CTACACATTT 
GAATCAGCTG TTAAAGTGAT GGCACGAGGT TTCATTTTAT GCCCGTTTAC GTATCTTAGA 
GATGCATGGA ATTGGCTGGA CTTCGTAGTA ATAGCTTTAG CTTATGTGAC CATGGGTATA 
GATTTAGGTA ATCTAGCAGC CCTGCGAACG TTTAGGGTGC TGCGAGCGCT TAAAACCGTA 
GCCATTGTGC CAGGCTTGAA GACCATCGTC GGCGCCGTCA TCGAATCGGT GAAGAATCTG 
CGCGATGTGA TTATCCTGAC CATGTTCTCC CTGTCGGTGT TCGCGTTGAT GGGCCTACAG 
ATCTATATGG GCGTGCTCAC GCAGAAGTGC ATCAAGAAGT TCCCGCTGGA CGGTTCCTGG 
GGCAATCTGA CCGACGAGAA CTGGGACTAT CACAATCGCA ATAGCTCCAA TTGGTATTCC 
GAGGACGAGG GCATCTCATT TCCGTTATGC GGCAATATAT CCGGTGCGGG GCAATGCGAC 
GACGATTACG TGTGCCTGCA GGGGTTTGGT CCGAATCCGA ATTATGGCTA CACCAGCTTC 
GATTCGTTCG GATGGGCTTT CCTGTCCGCC TTCCGGCTGA TGACACAGGA CTTCTGGGAG 
GATCTGTACC AGCTGGTGTT GCGCGCCGCC GGACCATGGC ACATGCTGTT CTTTATAGTC 
ATCATCTTCC TAGGTTCATT CTATCTTGTG AATTTGATTT TGGCCATTGT TGCCATGTCG 
TATGACGAAT TGCAAAAGAA GGCCGAAGAA GAAGAGGCTG CCGAAGAGGA GGCGATACGT 
GAAGCGGAAG AAGCTGCCGC CGCCAAAGCG GCCAAGCTGG AGGAGCGGGC CAATGCGCAG 
GCTCAGGCAG CAGCGGATGC GGCTGCCGCC GAAGAGGCTG CACTGCATCC GGAAATGGCC 
AAGAGTCCGA CGTATTCTTG CATCAGCTAT GAGCTATTTG TTGGCGGCGA GAAGGGCAAC 
GATGACAACA ACAAAGAGAA GATGTCCATT CGGAGCGTCG AGGTGGAGTC GGAGTCGGTG 
AGCGTTATAC AAAGACAACC AGCACCTACC ACAGCACACC AAGCTACCAA AGTTCGTAAA 
GTGAGCACGA CATCCTTATC CTTACCTGGT TCACCGTTTA ACATACGCAG GGGATCACGT 
AGTTCTCACA AGTACACGAT ACGGAACGGA CGTGGCCGCT TTGGTATACC CGGTAGCGAT 
CGTAAGCCAT TGGTATTGTC AACATATCAG GATGCCCAGC AGCACTTGCC CTATGCCGAC 
GACTCGAATG CCGTCACCCC GATGTCCGAA GAGAATGGGG CCATCATAGT GCCCGTGTAC 
TATGGCAATC TAGGCTCCCG ACACTCATCG TATACCTCGC ATCAGTCCCG AATATCGTAT 
ACCTCACATG GCGATCTACT CGGCGGCATG GCCGTCATGG GCGTCAGCAC AATGACCAAG 
GAGAGCAAAT TGCGCAACCG GAACACACGC AATCAATCAG TGGGCGCCAC CAATGGCGGC 
ACCACCTGTC TGGACACCAA TCACAAGCTC GATCATCGCG ACTACGAAAT TGGCCTGGAG 
TGCACGGACG AAGCTGGCAA GATTAAACAT CATGACAATC CTTTTATCGA GCCCGTCCAG 
ACACAAACGG TGGTTGATAT GAAAGATGTG ATGGTCCTGA ATGACATCAT CGAACAGGCC 
GCTGGTCGGC ACAGTCGGGC AAGCGATCGC GGTGTCTCCG TTTACTATTT CCCAACAGAG 
GACGATGACG AGGATGGGCC GACGTTCAAA GACAAGGCAC TCGAAGTGAT CCTCAAAGGC 
ATCGATGTGT TTTGTGTGTG GGACTGTTGC TGGGTTTGGT TGAAATTTCA GGAGTGGGTA 
TCGCTCATCG TCTTCGATCC CTTCGTCGAG CTCTTCATCA CGCTGTGCAT TGTGGTCAAC 
ACGATGTTCA TGGCAATGGA TCACCACGAT ATGAACAAGG AGATGGAACG CGTGCTCAAG 
AGTGGCAACT ATTTCTTCAC CGCCACCTTT GCCATCGAGG CCACCATGAA GCTAATGGCC 
ATGAGCCCCA AGTACTATTT CCAGGAGGGC TGGAACATCT TCGACTTCAT TATCGTGGCC 
CTATCGCTAT TGGAACTGGG ACTCGAGGGT GTCCAGGGTC TGTCCGTATT GCGTTCCTTT 
CGATTGCTGC GTGTTTTCAA GCTGGCCAAA TCGTGGCCGA CGCTGAATTT GCTCATATCG 
ATTATGGGCC GCACTGTCGG CGCCCTCGGC AATCTGACCT TCGTCCTGTG CATTATTATC 
TTCATATTCG CGGTCATGGG CATGCAGCTG TTTGGCAAGA ACTACACAGA TCACAAGGAC 
CGCTTTCCGG ATGGCGACCT GCCGCGCTGG AACTTCACCG ACTTTATGCA CAGCTTCATG 
ATCGTGTTCC GGGTGCTCTG CGGAGAATGG ATCGAGTCCA TGTGGGACTG CATGTACGTG 
GGCGATGTCT CGTGCATTCC CTTCTTCTTG GCCACCGTTG TCATCGGCAA TCTTGTGGTA 
CTTAACCTTT TCTTAGCCTT GCTTTTGTCC AATTTTGGCT CATCTAGCTT ATCAGCGCCG 
ACTGCCGATA ACGATACGAA TAAAATAGCC GAGGCCTTCA ATCGAATTGG CCGATTTAAA 
AGTTGGGTTA AGCGTAATAT TGCTGATTGT TTCAAGTTAA TACGTAACAA ATTGACAAAT 
CAAATAAGTG ATCAACCATC AGGCAAAGGC GTTTGTCGTT GTATATCTGC AGAGCATGGT 
GACAACGAAC TGGAGCTGGG CCACGACGAG ATCCTCGCCG ACGGCCTCAT CAAGAAGGGG 
ATCAAGGAGC AGACGCAACT GGAGGTGGCC ATCGGGGATG GCATGGAATT CACGATACAC 
GGCGACATGA AGAACAACAA GCCGAAGAAA TCCAAATATC TAAATAACGC AACGATGATT 
GGCAACTCAA TTAACCACCA AGACAATAGA CTGGAACACG AGCTAAACCA TAGAGGTTTG 
TCCTTACAGG ACGACGACAC TGCCAGCATT AACTCATATG GTAGCCATAA GAATCGACCA 
TTCAAGGACG AGAGCCACAA GGGCAGCGCC GAGACGATGG AGGGCGAGGA GAAGCGCGAC 
GCCAGCAAGG AGGATTTAGG TCTCGACGAG GAACTGGACG AGGAGGGCGA ATGCGAGGAG 
GGCCCGCTCG ACGGTGATAT CATTATTCAT GCACACGACG AGGATATACT CGATGAATAT 
CCAGCTGATT GCTGCCCCGA TTCGTACTAT AAGAAATTTC CGATCTTAGC CGGTGACGAT 
GACTCGCCGT TCTGGCAAGG ATGGGGCAAT TTACGACTGA AAACTTTTCA ATTAATTGAA 
AATAAATATT TTGAAACAGC TGTTATCACT ATGATTTTAA TGAGTAGCTT AGCTTTGGCA 
TTAGAAGATG TACATCTGCC ACAAAGACCC ATACTGCAGG ATATTTTATA CTATATGGAC 
AGAATATTTA CGGTTATATT CTTCTTGGAA ATGTTAATCA AGTGGTTGGC GCTCGGCTTC 
AAAGTGTACT TCACCAACGC GTGGTGTTGG CTCGATTTCG TGATTGTCAT GGTATCGCTT 
ATCAACTTCG TTGCTTCACT TGTTGGAGCT GGTGGTATTC AAGCCTTCAA GACTATGCGA 
ACGTTAAGAG CACTGAGACC ACTACGTGCC ATGTCCCGTA TGCAGGGCAT GAGGGTCGTC 
GTTAATGCGC TGGTACAAGC TATACCGTCC ATCTTCAATG TGCTATTGGT GTGTCTAATA 
TTTTGGCTAA TTTTTGCCAT AATGGGTGTA CAGCTTTTTG CTGGAAAATA TTTTAAGTGC 
GAGGACATGA ATGGCACGAA GCTCAGCCAC GAGATCATAC CAAATCGCAA TGCCTGCGAG 
AGCGAGAACT ACACGTGGGT GAATTCAGCA ATGAATTTCG ATCATGTAGG TAACGCGTAT 
CTGTGCCTTT TCCAAGTGGC CACCTTCAAA GGCTGGATAC AAATCATGAA CGATGCTATC 
GATTCACGAG AGGTGGACAA GCAACCAATT CGTGAAACGA ACATCTACAT GTATTTATAT 
TTCGTATTCT TCATCATATT TGGATCATTT TTCACACTCA ATCTGTTCAT TGGTGTTATC 
ATTGATAATT TTAATGAGCA AAAGAAAAAA GCAGGTGGAT CATTAGAAAT GTTCATGACA 
GAAGATCAGA AAAAGTACTA TAATGCTATG AAAAAGATGG GCTCTAAAAA ACCATTAAAA 
GCCATTCCAA GACCAAGGTG GCGACCACAA GCAATAGTCT TTGAAATAGT AACCGATAAG 
AAATTCGATA TAATCATTAT GTTATTCATT GGTCTGAACA TGTTCACCAT GACCCTCGAT 
CGTTACGATG CGTCGGACAC GTATAACGCG GTCCTAGACT ATCTCAATGC GATATTCGTA 
GTTATTTTCA GTTCCGAATG TCTATTAAAA ATATTCGCTT TACGATATCA CTATTTTATT 
GAGCCATGGA ATTTATTTGA TGTAGTAGTT GTCATTTTAT CCATCTTAGG TCTTGTACTT 
AGCGATATTA TCGAGAAGTA CTTCGTGTCG CCGACCCTGC TCCGAGTGGT GCGTGTGGCG 
AAAGTGGGCC GTGTCCTTCG ACTGGTGAAG GGAGCCAAGG GCATTCGGAC ACTGCTCTTC 
GCGTTGGCCA TGTCGCTGCC GGCCCTGTTC AACATCTGCC TGCTGCTGTT CCTGGTCATG 
TTCATCTTTG CCATTTTCGG CATGTCGTTC TTCATGCACG TGAAGGAGAA GAGCGGCATC 
AACGACGTCT ACAACTTCAA GACCTTTGGC CAGAGCATGA TCCTGCTCTT TCAGATGTCG 
ACGTCAGCCG GTTGGGATGG TGTACTGGAC GCCATTATCA ATGAGGAAGC ATGCGATCCA 
CCCGACAACG ACAAAGGCTA TCCGGGCAAT TGTGGTTCAG CGACCGTTGG AATAACGTTT 
CTCCTCTCAT ACCTAGTTAT AAGCTTTTTG ATAGTTATTA ATATGTACAT TGCTGTCATT 
CTCGAGAACT ATAGTCAGGC CACCGAGGAC GTGCAAGAGG GTCTAACCGA CGACGACTAC 
GACATGTACT ATGAGATCTG GCAGCAATTC GATCCGGAGG GCACCCAGTA CATACGCTAT 
GATCAGCTGT CCGAATTCCT GGACGTACTG GAGCCCCCGC TGCAGATCCA CAAACCGAAC 
AAGTACAAGA TCATATCGAT GGACATACCC ATCTGTCGCG GTGACCTCAT GTACTGCGTC 
GACATCCTCG ACGCCCTTAC GAAAGACTTC TTTGCGCGGA AGGGCAATCC GATAGAGGAG 
ACGGGTGAGA TTGGTGAGAT AGCGGCCCGC CCGGATACGG AGGGCTACGA GCCCGTCTCA 
TCAACGCTGT GGCGTCAGCG TGAGGAGTAC TGCGCCCGGC TAATCCAGCA CGCCTGGCGA 
AAGCACAAGG CGCGCGGCGA GGGAGGTGGG TCCTTTGAGC CGGATACGGA TCATGGCGAT 
GGCGGTGATC CGGATGCCGG GGACCCGGCG CCCGATGAAG CAACGGACGG CGATGCGCCC 
GCTGGTGGAG ATGGTAGTGT TAACGGTACT GCAGAAGGAG CTGCCGATGC CGATGAGAGT 
AATGTAAATA GTCCGGGTGA GGATGCAGCG GCGGCGGCAG CAGCAGCAGC AGCAGCGGCG 
GCGGCGGGCA CGACGACGGC GGGAAGTCCC GGAGCGGGTA GCGCCGGGCG ACAGACCGCC 
GTTCTCGTGG AGAGCGACGG GTTCGTGACG AAGAACGGCC ACAAGGTGGT CATCCACTCG 
CGATCGCCGA GCATCACGTC GCGCACGGCG GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201683.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188612.1 Drosophila melanogaster paralytic (para), transcript variant Q, mRNA.

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CloneID	OFa03666
Clone ID Related Accession (Same CDS sequence)	NM_001201683.1
Accession Version	NM_001201683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform Q
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGA GCATGGTGAC AACGAACTGG AGCTGGGCCA CGACGAGATC 
CTCGCCGACG GCCTCATCAA GAAGGGGATC AAGGAGCAGA CGCAACTGGA GGTGGCCATC 
GGGGATGGCA TGGAATTCAC GATACACGGC GACATGAAGA ACAACAAGCC GAAGAAATCC 
AAATATCTAA ATAACGCAAC GGACGACGAC ACTGCCAGCA TTAACTCATA TGGTAGCCAT 
AAGAATCGAC CATTCAAGGA CGAGAGCCAC AAGGGCAGCG CCGAGACGAT GGAGGGCGAG 
GAGAAGCGCG ACGCCAGCAA GGAGGATTTA GGTCTCGACG AGGAACTGGA CGAGGAGGGC 
GAATGCGAGG AGGGCCCGCT CGACGGTGAT ATCATTATTC ATGCACACGA CGAGGATATA 
CTCGATGAAT ATCCAGCTGA TTGCTGCCCC GATTCGTACT ATAAGAAATT TCCGATCTTA 
GCCGGTGACG ATGACTCGCC GTTCTGGCAA GGATGGGGCA ATTTACGACT GAAAACTTTT 
CAATTAATTG AAAATAAATA TTTTGAAACA GCTGTTATCA CTATGATTTT AATGAGTAGC 
TTAGCTTTGG CATTAGAAGA TGTACATCTG CCACAAAGAC CCATACTGCA GGATATTTTA 
TACTATATGG ACAGAATATT TACGGTTATA TTCTTCTTGG AAATGTTAAT CAAGTGGTTG 
GCGCTCGGCT TCAAAGTGTA CTTCACCAAC GCGTGGTGTT GGCTCGATTT CGTGATTGTC 
ATGGTATCGC TTATCAACTT CGTTGCTTCA CTTGTTGGAG CTGGTGGTAT TCAAGCCTTC 
AAGACTATGC GAACGTTAAG AGCACTGAGA CCACTACGTG CCATGTCCCG TATGCAGGGC 
ATGAGGGTCG TCGTTAATGC GCTGGTACAA GCTATACCGT CCATCTTCAA TGTGCTATTG 
GTGTGTCTAA TATTTTGGCT AATTTTTGCC ATAATGGGTG TACAGCTTTT TGCTGGAAAA 
TATTTTAAGT GCGAGGACAT GAATGGCACG AAGCTCAGCC ACGAGATCAT ACCAAATCGC 
AATGCCTGCG AGAGCGAGAA CTACACGTGG GTGAATTCAG CAATGAATTT CGATCATGTA 
GGTAACGCGT ATCTGTGCCT TTTCCAAGTG GCCACCTTCA AAGGCTGGAT ACAAATCATG 
AACGATGCTA TCGATTCACG AGAGGTGGAC AAGCAACCAA TTCGTGAAAC GAACATCTAC 
ATGTATTTAT ATTTCGTATT CTTCATCATA TTTGGATCAT TTTTCACACT CAATCTGTTC 
ATTGGTGTTA TCATTGATAA TTTTAATGAG CAAAAGAAAA AAGCAGGTGG ATCATTAGAA 
ATGTTCATGA CAGAAGATCA GAAAAAGTAC TATAATGCTA TGAAAAAGAT GGGCTCTAAA 
AAACCATTAA AAGCCATTCC AAGACCAAGG TGGCGACCAC AAGCAATAGT CTTTGAAATA 
GTAACCGATA AGAAATTCGA TATAATCATT ATGTTATTCA TTGGTCTGAA CATGTTCACC 
ATGACCCTCG ATCGTTACGA TGCGTCGGAC ACGTATAACG CGGTCCTAGA CTATCTCAAT 
GCGATATTCG TAGTTATTTT CAGTTCCGAA TGTCTATTAA AAATATTCGC TTTACGATAT 
CACTATTTTA TTGAGCCATG GAATTTATTT GATGTAGTAG TTGTCATTTT ATCCATCTTA 
GGTCTTGTAC TTAGCGATAT TATCGAGAAG TACTTCGTGT CGCCGACCCT GCTCCGAGTG 
GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT CGACTGGTGA AGGGAGCCAA GGGCATTCGG 
ACACTGCTCT TCGCGTTGGC CATGTCGCTG CCGGCCCTGT TCAACATCTG CCTGCTGCTG 
TTCCTGGTCA TGTTCATCTT TGCCATTTTC GGCATGTCGT TCTTCATGCA CGTGAAGGAG 
AAGAGCGGCA TCAACGACGT CTACAACTTC AAGACCTTTG GCCAGAGCAT GATCCTGCTC 
TTTCAGATGT CGACGTCAGC CGGTTGGGAT GGTGTACTGG ACGCCATTAT CAATGAGGAA 
GCATGCGATC CACCCGACAA CGACAAAGGC TATCCGGGCA ATTGTGGTTC AGCGACCGTT 
GGAATAACGT TTCTCCTCTC ATACCTAGTT ATAAGCTTTT TGATAGTTAT TAATATGTAC 
ATTGCTGTCA TTCTCGAGAA CTATAGTCAG GCCACCGAGG ACGTGCAAGA GGGTCTAACC 
GACGACGACT ACGACATGTA CTATGAGATC TGGCAGCAAT TCGATCCGGA GGGCACCCAG 
TACATACGCT ATGATCAGCT GTCCGAATTC CTGGACGTAC TGGAGCCCCC GCTGCAGATC 
CACAAACCGA ACAAGTACAA GATCATATCG ATGGACATAC CCATCTGTCG CGGTGACCTC 
ATGTACTGCG TCGACATCCT CGACGCCCTT ACGAAAGACT TCTTTGCGCG GAAGGGCAAT 
CCGATAGAGG AGACGGGTGA GATTGGTGAG ATAGCGGCCC GCCCGGATAC GGAGGGCTAC 
GAGCCCGTCT CATCAACGCT GTGGCGTCAG CGTGAGGAGT ACTGCGCCCG GCTAATCCAG 
CACGCCTGGC GAAAGCACAA GGCGCGCGGC GAGGGAGGTG GGTCCTTTGA GCCGGATACG 
GATCATGGCG ATGGCGGTGA TCCGGATGCC GGGGACCCGG CGCCCGATGA AGCAACGGAC 
GGCGATGCGC CCGCTGGTGG AGATGGTAGT GTTAACGGTA CTGCAGAAGG AGCTGCCGAT 
GCCGATGAGA GTAATGTAAA TAGTCCGGGT GAGGATGCAG CGGCGGCGGC AGCAGCAGCA 
GCAGCAGCGG CGGCGGCGGG CACGACGACG GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG 
CGACAGACCG CCGTTCTCGT GGAGAGCGAC GGGTTCGTGA CGAAGAACGG CCACAAGGTG 
GTCATCCACT CGCGATCGCC GAGCATCACG TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188612.1
CDS	269..6499
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201683.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant Q, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201683.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGA GCATGGTGAC AACGAACTGG AGCTGGGCCA CGACGAGATC 
CTCGCCGACG GCCTCATCAA GAAGGGGATC AAGGAGCAGA CGCAACTGGA GGTGGCCATC 
GGGGATGGCA TGGAATTCAC GATACACGGC GACATGAAGA ACAACAAGCC GAAGAAATCC 
AAATATCTAA ATAACGCAAC GGACGACGAC ACTGCCAGCA TTAACTCATA TGGTAGCCAT 
AAGAATCGAC CATTCAAGGA CGAGAGCCAC AAGGGCAGCG CCGAGACGAT GGAGGGCGAG 
GAGAAGCGCG ACGCCAGCAA GGAGGATTTA GGTCTCGACG AGGAACTGGA CGAGGAGGGC 
GAATGCGAGG AGGGCCCGCT CGACGGTGAT ATCATTATTC ATGCACACGA CGAGGATATA 
CTCGATGAAT ATCCAGCTGA TTGCTGCCCC GATTCGTACT ATAAGAAATT TCCGATCTTA 
GCCGGTGACG ATGACTCGCC GTTCTGGCAA GGATGGGGCA ATTTACGACT GAAAACTTTT 
CAATTAATTG AAAATAAATA TTTTGAAACA GCTGTTATCA CTATGATTTT AATGAGTAGC 
TTAGCTTTGG CATTAGAAGA TGTACATCTG CCACAAAGAC CCATACTGCA GGATATTTTA 
TACTATATGG ACAGAATATT TACGGTTATA TTCTTCTTGG AAATGTTAAT CAAGTGGTTG 
GCGCTCGGCT TCAAAGTGTA CTTCACCAAC GCGTGGTGTT GGCTCGATTT CGTGATTGTC 
ATGGTATCGC TTATCAACTT CGTTGCTTCA CTTGTTGGAG CTGGTGGTAT TCAAGCCTTC 
AAGACTATGC GAACGTTAAG AGCACTGAGA CCACTACGTG CCATGTCCCG TATGCAGGGC 
ATGAGGGTCG TCGTTAATGC GCTGGTACAA GCTATACCGT CCATCTTCAA TGTGCTATTG 
GTGTGTCTAA TATTTTGGCT AATTTTTGCC ATAATGGGTG TACAGCTTTT TGCTGGAAAA 
TATTTTAAGT GCGAGGACAT GAATGGCACG AAGCTCAGCC ACGAGATCAT ACCAAATCGC 
AATGCCTGCG AGAGCGAGAA CTACACGTGG GTGAATTCAG CAATGAATTT CGATCATGTA 
GGTAACGCGT ATCTGTGCCT TTTCCAAGTG GCCACCTTCA AAGGCTGGAT ACAAATCATG 
AACGATGCTA TCGATTCACG AGAGGTGGAC AAGCAACCAA TTCGTGAAAC GAACATCTAC 
ATGTATTTAT ATTTCGTATT CTTCATCATA TTTGGATCAT TTTTCACACT CAATCTGTTC 
ATTGGTGTTA TCATTGATAA TTTTAATGAG CAAAAGAAAA AAGCAGGTGG ATCATTAGAA 
ATGTTCATGA CAGAAGATCA GAAAAAGTAC TATAATGCTA TGAAAAAGAT GGGCTCTAAA 
AAACCATTAA AAGCCATTCC AAGACCAAGG TGGCGACCAC AAGCAATAGT CTTTGAAATA 
GTAACCGATA AGAAATTCGA TATAATCATT ATGTTATTCA TTGGTCTGAA CATGTTCACC 
ATGACCCTCG ATCGTTACGA TGCGTCGGAC ACGTATAACG CGGTCCTAGA CTATCTCAAT 
GCGATATTCG TAGTTATTTT CAGTTCCGAA TGTCTATTAA AAATATTCGC TTTACGATAT 
CACTATTTTA TTGAGCCATG GAATTTATTT GATGTAGTAG TTGTCATTTT ATCCATCTTA 
GGTCTTGTAC TTAGCGATAT TATCGAGAAG TACTTCGTGT CGCCGACCCT GCTCCGAGTG 
GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT CGACTGGTGA AGGGAGCCAA GGGCATTCGG 
ACACTGCTCT TCGCGTTGGC CATGTCGCTG CCGGCCCTGT TCAACATCTG CCTGCTGCTG 
TTCCTGGTCA TGTTCATCTT TGCCATTTTC GGCATGTCGT TCTTCATGCA CGTGAAGGAG 
AAGAGCGGCA TCAACGACGT CTACAACTTC AAGACCTTTG GCCAGAGCAT GATCCTGCTC 
TTTCAGATGT CGACGTCAGC CGGTTGGGAT GGTGTACTGG ACGCCATTAT CAATGAGGAA 
GCATGCGATC CACCCGACAA CGACAAAGGC TATCCGGGCA ATTGTGGTTC AGCGACCGTT 
GGAATAACGT TTCTCCTCTC ATACCTAGTT ATAAGCTTTT TGATAGTTAT TAATATGTAC 
ATTGCTGTCA TTCTCGAGAA CTATAGTCAG GCCACCGAGG ACGTGCAAGA GGGTCTAACC 
GACGACGACT ACGACATGTA CTATGAGATC TGGCAGCAAT TCGATCCGGA GGGCACCCAG 
TACATACGCT ATGATCAGCT GTCCGAATTC CTGGACGTAC TGGAGCCCCC GCTGCAGATC 
CACAAACCGA ACAAGTACAA GATCATATCG ATGGACATAC CCATCTGTCG CGGTGACCTC 
ATGTACTGCG TCGACATCCT CGACGCCCTT ACGAAAGACT TCTTTGCGCG GAAGGGCAAT 
CCGATAGAGG AGACGGGTGA GATTGGTGAG ATAGCGGCCC GCCCGGATAC GGAGGGCTAC 
GAGCCCGTCT CATCAACGCT GTGGCGTCAG CGTGAGGAGT ACTGCGCCCG GCTAATCCAG 
CACGCCTGGC GAAAGCACAA GGCGCGCGGC GAGGGAGGTG GGTCCTTTGA GCCGGATACG 
GATCATGGCG ATGGCGGTGA TCCGGATGCC GGGGACCCGG CGCCCGATGA AGCAACGGAC 
GGCGATGCGC CCGCTGGTGG AGATGGTAGT GTTAACGGTA CTGCAGAAGG AGCTGCCGAT 
GCCGATGAGA GTAATGTAAA TAGTCCGGGT GAGGATGCAG CGGCGGCGGC AGCAGCAGCA 
GCAGCAGCGG CGGCGGCGGG CACGACGACG GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG 
CGACAGACCG CCGTTCTCGT GGAGAGCGAC GGGTTCGTGA CGAAGAACGG CCACAAGGTG 
GTCATCCACT CGCGATCGCC GAGCATCACG TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201677.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188606.1 Drosophila melanogaster paralytic (para), transcript variant K, mRNA.

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CloneID	OFa03667
Clone ID Related Accession (Same CDS sequence)	NM_001201677.1
Accession Version	NM_001201677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TTTTCAAGCT GGCCAAATCG TGGCCGACGC TGAATTTGCT CATATCGATT 
ATGGGCCGCA CTGTCGGCGC CCTCGGCAAT CTGACCTTCG TCCTGTGCAT TATTATCTTC 
ATATTCGCGG TCATGGGCAT GCAGCTGTTT GGCAAGAACT ACACAGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGA GCATGGTGAC AACGAACTGG AGCTGGGCCA CGACGAGATC 
CTCGCCGACG GCCTCATCAA GAAGGGGATC AAGGAGCAGA CGCAACTGGA GGTGGCCATC 
GGGGATGGCA TGGAATTCAC GATACACGGC GACATGAAGA ACAACAAGCC GAAGAAATCC 
AAATATCTAA ATAACGCAAC GGACGACGAC ACTGCCAGCA TTAACTCATA TGGTAGCCAT 
AAGAATCGAC CATTCAAGGA CGAGAGCCAC AAGGGCAGCG CCGAGACGAT GGAGGGCGAG 
GAGAAGCGCG ACGCCAGCAA GGAGGATTTA GGTCTCGACG AGGAACTGGA CGAGGAGGGC 
GAATGCGAGG AGGGCCCGCT CGACGGTGAT ATCATTATTC ATGCACACGA CGAGGATATA 
CTCGATGAAT ATCCAGCTGA TTGCTGCCCC GATTCGTACT ATAAGAAATT TCCGATCTTA 
GCCGGTGACG ATGACTCGCC GTTCTGGCAA GGATGGGGCA ATTTACGACT GAAAACTTTT 
CAATTAATTG AAAATAAATA TTTTGAAACA GCTGTTATCA CTATGATTTT AATGAGTAGC 
TTAGCTTTGG CATTAGAAGA TGTACATCTG CCACAAAGAC CCATACTGCA GGATATTTTA 
TACTATATGG ACAGAATATT TACGGTTATA TTCTTCTTGG AAATGTTAAT CAAGTGGTTG 
GCGCTCGGCT TCAAAGTGTA CTTCACCAAC GCGTGGTGTT GGCTCGATTT CGTGATTGTC 
ATGGTATCGC TTATCAACTT CGTTGCTTCA CTTGTTGGAG CTGGTGGTAT TCAAGCCTTC 
AAGACTATGC GAACGTTAAG AGCACTGAGA CCACTACGTG CCATGTCCCG TATGCAGGGC 
ATGAGGGTCG TCGTTAATGC GCTGGTACAA GCTATACCGT CCATCTTCAA TGTGCTATTG 
GTGTGTCTAA TATTTTGGCT AATTTTTGCC ATAATGGGTG TACAGCTTTT TGCTGGAAAA 
TATTTTAAGT GCGAGGACAT GAATGGCACG AAGCTCAGCC ACGAGATCAT ACCAAATCGC 
AATGCCTGCG AGAGCGAGAA CTACACGTGG GTGAATTCAG CAATGAATTT CGATCATGTA 
GGTAACGCGT ATCTGTGCCT TTTCCAAGTG GCCACCTTCA AAGGCTGGAT ACAAATCATG 
AACGATGCTA TCGATTCACG AGAGGTGGAC AAGCAACCAA TTCGTGAAAC GAACATCTAC 
ATGTATTTAT ATTTCGTATT CTTCATCATA TTTGGATCAT TTTTCACACT CAATCTGTTC 
ATTGGTGTTA TCATTGATAA TTTTAATGAG CAAAAGAAAA AAGCAGGTGG ATCATTAGAA 
ATGTTCATGA CAGAAGATCA GAAAAAGTAC TATAATGCTA TGAAAAAGAT GGGCTCTAAA 
AAACCATTAA AAGCCATTCC AAGACCAAGG TGGCGACCAC AAGCAATAGT CTTTGAAATA 
GTAACCGATA AGAAATTCGA TATAATCATT ATGTTATTCA TTGGTCTGAA CATGTTCACC 
ATGACCCTCG ATCGTTACGA TGCGTCGGAC ACGTATAACG CGGTCCTAGA CTATCTCAAT 
GCGATATTCG TAGTTATTTT CAGTTCCGAA TGTCTATTAA AAATATTCGC TTTACGATAT 
CACTATTTTA TTGAGCCATG GAATTTATTT GATGTAGTAG TTGTCATTTT ATCCATCTTA 
GGTCTTGTAC TTAGCGATAT TATCGAGAAG TACTTCGTGT CGCCGACCCT GCTCCGAGTG 
GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT CGACTGGTGA AGGGAGCCAA GGGCATTCGG 
ACACTGCTCT TCGCGTTGGC CATGTCGCTG CCGGCCCTGT TCAACATCTG CCTGCTGCTG 
TTCCTGGTCA TGTTCATCTT TGCCATTTTC GGCATGTCGT TCTTCATGCA CGTGAAGGAG 
AAGAGCGGCA TCAACGACGT CTACAACTTC AAGACCTTTG GCCAGAGCAT GATCCTGCTC 
TTTCAGATGT CGACGTCAGC CGGTTGGGAT GGTGTACTGG ACGCCATTAT CAATGAGGAA 
GCATGCGATC CACCCGACAA CGACAAAGGC TATCCGGGCA ATTGTGGTTC AGCGACCGTT 
GGAATAACGT TTCTCCTCTC ATACCTAGTT ATAAGCTTTT TGATAGTTAT TAATATGTAC 
ATTGCTGTCA TTCTCGAGAA CTATAGTCAG GCCACCGAGG ACGTGCAAGA GGGTCTAACC 
GACGACGACT ACGACATGTA CTATGAGATC TGGCAGCAAT TCGATCCGGA GGGCACCCAG 
TACATACGCT ATGATCAGCT GTCCGAATTC CTGGACGTAC TGGAGCCCCC GCTGCAGATC 
CACAAACCGA ACAAGTACAA GATCATATCG ATGGACATAC CCATCTGTCG CGGTGACCTC 
ATGTACTGCG TCGACATCCT CGACGCCCTT ACGAAAGACT TCTTTGCGCG GAAGGGCAAT 
CCGATAGAGG AGACGGGTGA GATTGGTGAG ATAGCGGCCC GCCCGGATAC GGAGGGCTAC 
GAGCCCGTCT CATCAACGCT GTGGCGTCAG CGTGAGGAGT ACTGCGCCCG GCTAATCCAG 
CACGCCTGGC GAAAGCACAA GGCGCGCGGC GAGGGAGGTG GGTCCTTTGA GCCGGATACG 
GATCATGGCG ATGGCGGTGA TCCGGATGCC GGGGACCCGG CGCCCGATGA AGCAACGGAC 
GGCGATGCGC CCGCTGGTGG AGATGGTAGT GTTAACGGTA CTGCAGAAGG AGCTGCCGAT 
GCCGATGAGA GTAATGTAAA TAGTCCGGGT GAGGATGCAG CGGCGGCGGC AGCAGCAGCA 
GCAGCAGCGG CGGCGGCGGG CACGACGACG GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG 
CGACAGACCG CCGTTCTCGT GGAGAGCGAC GGGTTCGTGA CGAAGAACGG CCACAAGGTG 
GTCATCCACT CGCGATCGCC GAGCATCACG TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188606.1
CDS	269..6499
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201677.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant K, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201677.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TTTTCAAGCT GGCCAAATCG TGGCCGACGC TGAATTTGCT CATATCGATT 
ATGGGCCGCA CTGTCGGCGC CCTCGGCAAT CTGACCTTCG TCCTGTGCAT TATTATCTTC 
ATATTCGCGG TCATGGGCAT GCAGCTGTTT GGCAAGAACT ACACAGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGA GCATGGTGAC AACGAACTGG AGCTGGGCCA CGACGAGATC 
CTCGCCGACG GCCTCATCAA GAAGGGGATC AAGGAGCAGA CGCAACTGGA GGTGGCCATC 
GGGGATGGCA TGGAATTCAC GATACACGGC GACATGAAGA ACAACAAGCC GAAGAAATCC 
AAATATCTAA ATAACGCAAC GGACGACGAC ACTGCCAGCA TTAACTCATA TGGTAGCCAT 
AAGAATCGAC CATTCAAGGA CGAGAGCCAC AAGGGCAGCG CCGAGACGAT GGAGGGCGAG 
GAGAAGCGCG ACGCCAGCAA GGAGGATTTA GGTCTCGACG AGGAACTGGA CGAGGAGGGC 
GAATGCGAGG AGGGCCCGCT CGACGGTGAT ATCATTATTC ATGCACACGA CGAGGATATA 
CTCGATGAAT ATCCAGCTGA TTGCTGCCCC GATTCGTACT ATAAGAAATT TCCGATCTTA 
GCCGGTGACG ATGACTCGCC GTTCTGGCAA GGATGGGGCA ATTTACGACT GAAAACTTTT 
CAATTAATTG AAAATAAATA TTTTGAAACA GCTGTTATCA CTATGATTTT AATGAGTAGC 
TTAGCTTTGG CATTAGAAGA TGTACATCTG CCACAAAGAC CCATACTGCA GGATATTTTA 
TACTATATGG ACAGAATATT TACGGTTATA TTCTTCTTGG AAATGTTAAT CAAGTGGTTG 
GCGCTCGGCT TCAAAGTGTA CTTCACCAAC GCGTGGTGTT GGCTCGATTT CGTGATTGTC 
ATGGTATCGC TTATCAACTT CGTTGCTTCA CTTGTTGGAG CTGGTGGTAT TCAAGCCTTC 
AAGACTATGC GAACGTTAAG AGCACTGAGA CCACTACGTG CCATGTCCCG TATGCAGGGC 
ATGAGGGTCG TCGTTAATGC GCTGGTACAA GCTATACCGT CCATCTTCAA TGTGCTATTG 
GTGTGTCTAA TATTTTGGCT AATTTTTGCC ATAATGGGTG TACAGCTTTT TGCTGGAAAA 
TATTTTAAGT GCGAGGACAT GAATGGCACG AAGCTCAGCC ACGAGATCAT ACCAAATCGC 
AATGCCTGCG AGAGCGAGAA CTACACGTGG GTGAATTCAG CAATGAATTT CGATCATGTA 
GGTAACGCGT ATCTGTGCCT TTTCCAAGTG GCCACCTTCA AAGGCTGGAT ACAAATCATG 
AACGATGCTA TCGATTCACG AGAGGTGGAC AAGCAACCAA TTCGTGAAAC GAACATCTAC 
ATGTATTTAT ATTTCGTATT CTTCATCATA TTTGGATCAT TTTTCACACT CAATCTGTTC 
ATTGGTGTTA TCATTGATAA TTTTAATGAG CAAAAGAAAA AAGCAGGTGG ATCATTAGAA 
ATGTTCATGA CAGAAGATCA GAAAAAGTAC TATAATGCTA TGAAAAAGAT GGGCTCTAAA 
AAACCATTAA AAGCCATTCC AAGACCAAGG TGGCGACCAC AAGCAATAGT CTTTGAAATA 
GTAACCGATA AGAAATTCGA TATAATCATT ATGTTATTCA TTGGTCTGAA CATGTTCACC 
ATGACCCTCG ATCGTTACGA TGCGTCGGAC ACGTATAACG CGGTCCTAGA CTATCTCAAT 
GCGATATTCG TAGTTATTTT CAGTTCCGAA TGTCTATTAA AAATATTCGC TTTACGATAT 
CACTATTTTA TTGAGCCATG GAATTTATTT GATGTAGTAG TTGTCATTTT ATCCATCTTA 
GGTCTTGTAC TTAGCGATAT TATCGAGAAG TACTTCGTGT CGCCGACCCT GCTCCGAGTG 
GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT CGACTGGTGA AGGGAGCCAA GGGCATTCGG 
ACACTGCTCT TCGCGTTGGC CATGTCGCTG CCGGCCCTGT TCAACATCTG CCTGCTGCTG 
TTCCTGGTCA TGTTCATCTT TGCCATTTTC GGCATGTCGT TCTTCATGCA CGTGAAGGAG 
AAGAGCGGCA TCAACGACGT CTACAACTTC AAGACCTTTG GCCAGAGCAT GATCCTGCTC 
TTTCAGATGT CGACGTCAGC CGGTTGGGAT GGTGTACTGG ACGCCATTAT CAATGAGGAA 
GCATGCGATC CACCCGACAA CGACAAAGGC TATCCGGGCA ATTGTGGTTC AGCGACCGTT 
GGAATAACGT TTCTCCTCTC ATACCTAGTT ATAAGCTTTT TGATAGTTAT TAATATGTAC 
ATTGCTGTCA TTCTCGAGAA CTATAGTCAG GCCACCGAGG ACGTGCAAGA GGGTCTAACC 
GACGACGACT ACGACATGTA CTATGAGATC TGGCAGCAAT TCGATCCGGA GGGCACCCAG 
TACATACGCT ATGATCAGCT GTCCGAATTC CTGGACGTAC TGGAGCCCCC GCTGCAGATC 
CACAAACCGA ACAAGTACAA GATCATATCG ATGGACATAC CCATCTGTCG CGGTGACCTC 
ATGTACTGCG TCGACATCCT CGACGCCCTT ACGAAAGACT TCTTTGCGCG GAAGGGCAAT 
CCGATAGAGG AGACGGGTGA GATTGGTGAG ATAGCGGCCC GCCCGGATAC GGAGGGCTAC 
GAGCCCGTCT CATCAACGCT GTGGCGTCAG CGTGAGGAGT ACTGCGCCCG GCTAATCCAG 
CACGCCTGGC GAAAGCACAA GGCGCGCGGC GAGGGAGGTG GGTCCTTTGA GCCGGATACG 
GATCATGGCG ATGGCGGTGA TCCGGATGCC GGGGACCCGG CGCCCGATGA AGCAACGGAC 
GGCGATGCGC CCGCTGGTGG AGATGGTAGT GTTAACGGTA CTGCAGAAGG AGCTGCCGAT 
GCCGATGAGA GTAATGTAAA TAGTCCGGGT GAGGATGCAG CGGCGGCGGC AGCAGCAGCA 
GCAGCAGCGG CGGCGGCGGG CACGACGACG GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG 
CGACAGACCG CCGTTCTCGT GGAGAGCGAC GGGTTCGTGA CGAAGAACGG CCACAAGGTG 
GTCATCCACT CGCGATCGCC GAGCATCACG TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201694.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188623.1 Drosophila melanogaster paralytic (para), transcript variant AB, mRNA.

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CloneID	OFa03668
Clone ID Related Accession (Same CDS sequence)	NM_001201694.1
Accession Version	NM_001201694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AB
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TTTTCAAGCT GGCCAAATCG TGGCCGACGC TGAATTTGCT CATATCGATT 
ATGGGCCGCA CTGTCGGCGC CCTCGGCAAT CTGACCTTCG TCCTGTGCAT TATTATCTTC 
ATATTCGCGG TCATGGGCAT GCAGCTGTTT GGCAAGAACT ACACAGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG CAAAGGCGTT TGTCGTTGTA TATCTGCAGA GCATGGTGAC 
AACGAACTGG AGCTGGGCCA CGACGAGATC CTCGCCGACG GCCTCATCAA GAAGGGGATC 
AAGGAGCAGA CGCAACTGGA GGTGGCCATC GGGGATGGCA TGGAATTCAC GATACACGGC 
GACATGAAGA ACAACAAGCC GAAGAAATCC AAATATCTAA ATAACGCAAC GGACGACGAC 
ACTGCCAGCA TTAACTCATA TGGTAGCCAT AAGAATCGAC CATTCAAGGA CGAGAGCCAC 
AAGGGCAGCG CCGAGACGAT GGAGGGCGAG GAGAAGCGCG ACGCCAGCAA GGAGGATTTA 
GGTCTCGACG AGGAACTGGA CGAGGAGGGC GAATGCGAGG AGGGCCCGCT CGACGGTGAT 
ATCATTATTC ATGCACACGA CGAGGATATA CTCGATGAAT ATCCAGCTGA TTGCTGCCCC 
GATTCGTACT ATAAGAAATT TCCGATCTTA GCCGGTGACG ATGACTCGCC GTTCTGGCAA 
GGATGGGGCA ATTTACGACT GAAAACTTTT CAATTAATTG AAAATAAATA TTTTGAAACA 
GCTGTTATCA CTATGATTTT AATGAGTAGC TTAGCTTTGG CATTAGAAGA TGTACATCTG 
CCACAAAGAC CCATACTGCA GGATATTTTA TACTATATGG ACAGAATATT TACGGTTATA 
TTCTTCTTGG AAATGTTAAT CAAGTGGTTG GCGCTCGGCT TCAAAGTGTA CTTCACCAAC 
GCGTGGTGTT GGCTCGATTT CGTGATTGTC ATGGTATCGC TTATCAACTT CGTTGCTTCA 
CTTGTTGGAG CTGGTGGTAT TCAAGCCTTC AAGACTATGC GAACGTTAAG AGCACTGAGA 
CCACTACGTG CCATGTCCCG TATGCAGGGC ATGAGGGTCG TCGTTAATGC GCTGGTACAA 
GCTATACCGT CCATCTTCAA TGTGCTATTG GTGTGTCTAA TATTTTGGCT AATTTTTGCC 
ATAATGGGTG TACAGCTTTT TGCTGGAAAA TATTTTAAGT GCGAGGACAT GAATGGCACG 
AAGCTCAGCC ACGAGATCAT ACCAAATCGC AATGCCTGCG AGAGCGAGAA CTACACGTGG 
GTGAATTCAG CAATGAATTT CGATCATGTA GGTAACGCGT ATCTGTGCCT TTTCCAAGTG 
GCCACCTTCA AAGGCTGGAT ACAAATCATG AACGATGCTA TCGATTCACG AGAGGTGGAC 
AAGCAACCAA TTCGTGAAAC GAACATCTAC ATGTATTTAT ATTTCGTATT CTTCATCATA 
TTTGGATCAT TTTTCACACT CAATCTGTTC ATTGGTGTTA TCATTGATAA TTTTAATGAG 
CAAAAGAAAA AAGCAGGTGG ATCATTAGAA ATGTTCATGA CAGAAGATCA GAAAAAGTAC 
TATAATGCTA TGAAAAAGAT GGGCTCTAAA AAACCATTAA AAGCCATTCC AAGACCAAGG 
TGGCGACCAC AAGCAATAGT CTTTGAAATA GTAACCGATA AGAAATTCGA TATAATCATT 
ATGTTATTCA TTGGTCTGAA CATGTTCACC ATGACCCTCG ATCGTTACGA TGCGTCGGAC 
ACGTATAACG CGGTCCTAGA CTATCTCAAT GCGATATTCG TAGTTATTTT CAGTTCCGAA 
TGTCTATTAA AAATATTCGC TTTACGATAT CACTATTTTA TTGAGCCATG GAATTTATTT 
GATGTAGTAG TTGTCATTTT ATCCATCTTA GGTCTTGTAC TTAGCGATAT TATCGAGAAG 
TACTTCGTGT CGCCGACCCT GCTCCGAGTG GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT 
CGACTGGTGA AGGGAGCCAA GGGCATTCGG ACACTGCTCT TCGCGTTGGC CATGTCGCTG 
CCGGCCCTGT TCAACATCTG CCTGCTGCTG TTCCTGGTCA TGTTCATCTT TGCCATTTTC 
GGCATGTCGT TCTTCATGCA CGTGAAGGAG AAGAGCGGCA TCAACGACGT CTACAACTTC 
AAGACCTTTG GCCAGAGCAT GATCCTGCTC TTTCAGATGT CGACGTCAGC CGGTTGGGAT 
GGTGTACTGG ACGCCATTAT CAATGAGGAA GCATGCGATC CACCCGACAA CGACAAAGGC 
TATCCGGGCA ATTGTGGTTC AGCGACCGTT GGAATAACGT TTCTCCTCTC ATACCTAGTT 
ATAAGCTTTT TGATAGTTAT TAATATGTAC ATTGCTGTCA TTCTCGAGAA CTATAGTCAG 
GCCACCGAGG ACGTGCAAGA GGGTCTAACC GACGACGACT ACGACATGTA CTATGAGATC 
TGGCAGCAAT TCGATCCGGA GGGCACCCAG TACATACGCT ATGATCAGCT GTCCGAATTC 
CTGGACGTAC TGGAGCCCCC GCTGCAGATC CACAAACCGA ACAAGTACAA GATCATATCG 
ATGGACATAC CCATCTGTCG CGGTGACCTC ATGTACTGCG TCGACATCCT CGACGCCCTT 
ACGAAAGACT TCTTTGCGCG GAAGGGCAAT CCGATAGAGG AGACGGGTGA GATTGGTGAG 
ATAGCGGCCC GCCCGGATAC GGAGGGCTAC GAGCCCGTCT CATCAACGCT GTGGCGTCAG 
CGTGAGGAGT ACTGCGCCCG GCTAATCCAG CACGCCTGGC GAAAGCACAA GGCGCGCGGC 
GAGGGAGGTG GGTCCTTTGA GCCGGATACG GATCATGGCG ATGGCGGTGA TCCGGATGCC 
GGGGACCCGG CGCCCGATGA AGCAACGGAC GGCGATGCGC CCGCTGGTGG AGATGGTAGT 
GTTAACGGTA CTGCAGAAGG AGCTGCCGAT GCCGATGAGA GTAATGTAAA TAGTCCGGGT 
GAGGATGCAG CGGCGGCGGC AGCAGCAGCA GCAGCAGCGG CGGCGGCGGG CACGACGACG 
GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG CGACAGACCG CCGTTCTCGT GGAGAGCGAC 
GGGTTCGTGA CGAAGAACGG CCACAAGGTG GTCATCCACT CGCGATCGCC GAGCATCACG 
TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188623.1
CDS	269..6529
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201694.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AB, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201694.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TTTTCAAGCT GGCCAAATCG TGGCCGACGC TGAATTTGCT CATATCGATT 
ATGGGCCGCA CTGTCGGCGC CCTCGGCAAT CTGACCTTCG TCCTGTGCAT TATTATCTTC 
ATATTCGCGG TCATGGGCAT GCAGCTGTTT GGCAAGAACT ACACAGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG CAAAGGCGTT TGTCGTTGTA TATCTGCAGA GCATGGTGAC 
AACGAACTGG AGCTGGGCCA CGACGAGATC CTCGCCGACG GCCTCATCAA GAAGGGGATC 
AAGGAGCAGA CGCAACTGGA GGTGGCCATC GGGGATGGCA TGGAATTCAC GATACACGGC 
GACATGAAGA ACAACAAGCC GAAGAAATCC AAATATCTAA ATAACGCAAC GGACGACGAC 
ACTGCCAGCA TTAACTCATA TGGTAGCCAT AAGAATCGAC CATTCAAGGA CGAGAGCCAC 
AAGGGCAGCG CCGAGACGAT GGAGGGCGAG GAGAAGCGCG ACGCCAGCAA GGAGGATTTA 
GGTCTCGACG AGGAACTGGA CGAGGAGGGC GAATGCGAGG AGGGCCCGCT CGACGGTGAT 
ATCATTATTC ATGCACACGA CGAGGATATA CTCGATGAAT ATCCAGCTGA TTGCTGCCCC 
GATTCGTACT ATAAGAAATT TCCGATCTTA GCCGGTGACG ATGACTCGCC GTTCTGGCAA 
GGATGGGGCA ATTTACGACT GAAAACTTTT CAATTAATTG AAAATAAATA TTTTGAAACA 
GCTGTTATCA CTATGATTTT AATGAGTAGC TTAGCTTTGG CATTAGAAGA TGTACATCTG 
CCACAAAGAC CCATACTGCA GGATATTTTA TACTATATGG ACAGAATATT TACGGTTATA 
TTCTTCTTGG AAATGTTAAT CAAGTGGTTG GCGCTCGGCT TCAAAGTGTA CTTCACCAAC 
GCGTGGTGTT GGCTCGATTT CGTGATTGTC ATGGTATCGC TTATCAACTT CGTTGCTTCA 
CTTGTTGGAG CTGGTGGTAT TCAAGCCTTC AAGACTATGC GAACGTTAAG AGCACTGAGA 
CCACTACGTG CCATGTCCCG TATGCAGGGC ATGAGGGTCG TCGTTAATGC GCTGGTACAA 
GCTATACCGT CCATCTTCAA TGTGCTATTG GTGTGTCTAA TATTTTGGCT AATTTTTGCC 
ATAATGGGTG TACAGCTTTT TGCTGGAAAA TATTTTAAGT GCGAGGACAT GAATGGCACG 
AAGCTCAGCC ACGAGATCAT ACCAAATCGC AATGCCTGCG AGAGCGAGAA CTACACGTGG 
GTGAATTCAG CAATGAATTT CGATCATGTA GGTAACGCGT ATCTGTGCCT TTTCCAAGTG 
GCCACCTTCA AAGGCTGGAT ACAAATCATG AACGATGCTA TCGATTCACG AGAGGTGGAC 
AAGCAACCAA TTCGTGAAAC GAACATCTAC ATGTATTTAT ATTTCGTATT CTTCATCATA 
TTTGGATCAT TTTTCACACT CAATCTGTTC ATTGGTGTTA TCATTGATAA TTTTAATGAG 
CAAAAGAAAA AAGCAGGTGG ATCATTAGAA ATGTTCATGA CAGAAGATCA GAAAAAGTAC 
TATAATGCTA TGAAAAAGAT GGGCTCTAAA AAACCATTAA AAGCCATTCC AAGACCAAGG 
TGGCGACCAC AAGCAATAGT CTTTGAAATA GTAACCGATA AGAAATTCGA TATAATCATT 
ATGTTATTCA TTGGTCTGAA CATGTTCACC ATGACCCTCG ATCGTTACGA TGCGTCGGAC 
ACGTATAACG CGGTCCTAGA CTATCTCAAT GCGATATTCG TAGTTATTTT CAGTTCCGAA 
TGTCTATTAA AAATATTCGC TTTACGATAT CACTATTTTA TTGAGCCATG GAATTTATTT 
GATGTAGTAG TTGTCATTTT ATCCATCTTA GGTCTTGTAC TTAGCGATAT TATCGAGAAG 
TACTTCGTGT CGCCGACCCT GCTCCGAGTG GTGCGTGTGG CGAAAGTGGG CCGTGTCCTT 
CGACTGGTGA AGGGAGCCAA GGGCATTCGG ACACTGCTCT TCGCGTTGGC CATGTCGCTG 
CCGGCCCTGT TCAACATCTG CCTGCTGCTG TTCCTGGTCA TGTTCATCTT TGCCATTTTC 
GGCATGTCGT TCTTCATGCA CGTGAAGGAG AAGAGCGGCA TCAACGACGT CTACAACTTC 
AAGACCTTTG GCCAGAGCAT GATCCTGCTC TTTCAGATGT CGACGTCAGC CGGTTGGGAT 
GGTGTACTGG ACGCCATTAT CAATGAGGAA GCATGCGATC CACCCGACAA CGACAAAGGC 
TATCCGGGCA ATTGTGGTTC AGCGACCGTT GGAATAACGT TTCTCCTCTC ATACCTAGTT 
ATAAGCTTTT TGATAGTTAT TAATATGTAC ATTGCTGTCA TTCTCGAGAA CTATAGTCAG 
GCCACCGAGG ACGTGCAAGA GGGTCTAACC GACGACGACT ACGACATGTA CTATGAGATC 
TGGCAGCAAT TCGATCCGGA GGGCACCCAG TACATACGCT ATGATCAGCT GTCCGAATTC 
CTGGACGTAC TGGAGCCCCC GCTGCAGATC CACAAACCGA ACAAGTACAA GATCATATCG 
ATGGACATAC CCATCTGTCG CGGTGACCTC ATGTACTGCG TCGACATCCT CGACGCCCTT 
ACGAAAGACT TCTTTGCGCG GAAGGGCAAT CCGATAGAGG AGACGGGTGA GATTGGTGAG 
ATAGCGGCCC GCCCGGATAC GGAGGGCTAC GAGCCCGTCT CATCAACGCT GTGGCGTCAG 
CGTGAGGAGT ACTGCGCCCG GCTAATCCAG CACGCCTGGC GAAAGCACAA GGCGCGCGGC 
GAGGGAGGTG GGTCCTTTGA GCCGGATACG GATCATGGCG ATGGCGGTGA TCCGGATGCC 
GGGGACCCGG CGCCCGATGA AGCAACGGAC GGCGATGCGC CCGCTGGTGG AGATGGTAGT 
GTTAACGGTA CTGCAGAAGG AGCTGCCGAT GCCGATGAGA GTAATGTAAA TAGTCCGGGT 
GAGGATGCAG CGGCGGCGGC AGCAGCAGCA GCAGCAGCGG CGGCGGCGGG CACGACGACG 
GCGGGAAGTC CCGGAGCGGG TAGCGCCGGG CGACAGACCG CCGTTCTCGT GGAGAGCGAC 
GGGTTCGTGA CGAAGAACGG CCACAAGGTG GTCATCCACT CGCGATCGCC GAGCATCACG 
TCGCGCACGG CGGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201692.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188621.1 Drosophila melanogaster paralytic (para), transcript variant Z, mRNA.

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CloneID	OFa03669
Clone ID Related Accession (Same CDS sequence)	NM_001201692.1
Accession Version	NM_001201692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform Z
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TTTTCAAGCT GGCCAAATCG TGGCCGACGC TGAATTTGCT CATATCGATT 
ATGGGCCGCA CTGTCGGCGC CCTCGGCAAT CTGACCTTCG TCCTGTGCAT TATTATCTTC 
ATATTCGCGG TCATGGGCAT GCAGCTGTTT GGCAAGAACT ACACAGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG TGAGAGGACC AACCAGATCA GTTGGATTTG GAGCGAAGAG 
CATGGTGACA ACGAACTGGA GCTGGGCCAC GACGAGATCC TCGCCGACGG CCTCATCAAG 
AAGGGGATCA AGGAGCAGAC GCAACTGGAG GTGGCCATCG GGGATGGCAT GGAATTCACG 
ATACACGGCG ACATGAAGAA CAACAAGCCG AAGAAATCCA AATATCTAAA TAACGCAACG 
GACGACGACA CTGCCAGCAT TAACTCATAT GGTAGCCATA AGAATCGACC ATTCAAGGAC 
GAGAGCCACA AGGGCAGCGC CGAGACGATG GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG 
GAGGATTTAG GTCTCGACGA GGAACTGGAC GAGGAGGGCG AATGCGAGGA GGGCCCGCTC 
GACGGTGATA TCATTATTCA TGCACACGAC GAGGATATAC TCGATGAATA TCCAGCTGAT 
TGCTGCCCCG ATTCGTACTA TAAGAAATTT CCGATCTTAG CCGGTGACGA TGACTCGCCG 
TTCTGGCAAG GATGGGGCAA TTTACGACTG AAAACTTTTC AATTAATTGA AAATAAATAT 
TTTGAAACAG CTGTTATCAC TATGATTTTA ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT 
GTACATCTGC CACAAAGACC CATACTGCAG GATATTTTAT ACTATATGGA CAGAATATTT 
ACGGTTATAT TCTTCTTGGA AATGTTAATC AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC 
TTCACCAACG CGTGGTGTTG GCTCGATTTC GTGATTGTCA TGGTATCGCT TATCAACTTC 
GTTGCTTCAC TTGTTGGAGC TGGTGGTATT CAAGCCTTCA AGACTATGCG AACGTTAAGA 
GCACTGAGAC CACTACGTGC CATGTCCCGT ATGCAGGGCA TGAGGGTCGT CGTTAATGCG 
CTGGTACAAG CTATACCGTC CATCTTCAAT GTGCTATTGG TGTGTCTAAT ATTTTGGCTA 
ATTTTTGCCA TAATGGGTGT ACAGCTTTTT GCTGGAAAAT ATTTTAAGTG CGAGGACATG 
AATGGCACGA AGCTCAGCCA CGAGATCATA CCAAATCGCA ATGCCTGCGA GAGCGAGAAC 
TACACGTGGG TGAATTCAGC AATGAATTTC GATCATGTAG GTAACGCGTA TCTGTGCCTT 
TTCCAAGTGG CCACCTTCAA AGGCTGGATA CAAATCATGA ACGATGCTAT CGATTCACGA 
GAGGTGGACA AGCAACCAAT TCGTGAAACG AACATCTACA TGTATTTATA TTTCGTATTC 
TTCATCATAT TTGGATCATT TTTCACACTC AATCTGTTCA TTGGTGTTAT CATTGATAAT 
TTTAATGAGC AAAAGAAAAA AGCAGGTGGA TCATTAGAAA TGTTCATGAC AGAAGATCAG 
AAAAAGTACT ATAATGCTAT GAAAAAGATG GGCTCTAAAA AACCATTAAA AGCCATTCCA 
AGACCAAGGT GGCGACCACA AGCAATAGTC TTTGAAATAG TAACCGATAA GAAATTCGAT 
ATAATCATTA TGTTATTCAT TGGTCTGAAC ATGTTCACCA TGACCCTCGA TCGTTACGAT 
GCGTCGGACA CGTATAACGC GGTCCTAGAC TATCTCAATG CGATATTCGT AGTTATTTTC 
AGTTCCGAAT GTCTATTAAA AATATTCGCT TTACGATATC ACTATTTTAT TGAGCCATGG 
AATTTATTTG ATGTAGTAGT TGTCATTTTA TCCATCTTAG GTCTTGTACT TAGCGATATT 
ATCGAGAAGT ACTTCGTGTC GCCGACCCTG CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC 
CGTGTCCTTC GACTGGTGAA GGGAGCCAAG GGCATTCGGA CACTGCTCTT CGCGTTGGCC 
ATGTCGCTGC CGGCCCTGTT CAACATCTGC CTGCTGCTGT TCCTGGTCAT GTTCATCTTT 
GCCATTTTCG GCATGTCGTT CTTCATGCAC GTGAAGGAGA AGAGCGGCAT CAACGACGTC 
TACAACTTCA AGACCTTTGG CCAGAGCATG ATCCTGCTCT TTCAGATGTC GACGTCAGCC 
GGTTGGGATG GTGTACTGGA CGCCATTATC AATGAGGAAG CATGCGATCC ACCCGACAAC 
GACAAAGGCT ATCCGGGCAA TTGTGGTTCA GCGACCGTTG GAATAACGTT TCTCCTCTCA 
TACCTAGTTA TAAGCTTTTT GATAGTTATT AATATGTACA TTGCTGTCAT TCTCGAGAAC 
TATAGTCAGG CCACCGAGGA CGTGCAAGAG GGTCTAACCG ACGACGACTA CGACATGTAC 
TATGAGATCT GGCAGCAATT CGATCCGGAG GGCACCCAGT ACATACGCTA TGATCAGCTG 
TCCGAATTCC TGGACGTACT GGAGCCCCCG CTGCAGATCC ACAAACCGAA CAAGTACAAG 
ATCATATCGA TGGACATACC CATCTGTCGC GGTGACCTCA TGTACTGCGT CGACATCCTC 
GACGCCCTTA CGAAAGACTT CTTTGCGCGG AAGGGCAATC CGATAGAGGA GACGGGTGAG 
ATTGGTGAGA TAGCGGCCCG CCCGGATACG GAGGGCTACG AGCCCGTCTC ATCAACGCTG 
TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG CTAATCCAGC ACGCCTGGCG AAAGCACAAG 
GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG CCGGATACGG ATCATGGCGA TGGCGGTGAT 
CCGGATGCCG GGGACCCGGC GCCCGATGAA GCAACGGACG GCGATGCGCC CGCTGGTGGA 
GATGGTAGTG TTAACGGTAC TGCAGAAGGA GCTGCCGATG CCGATGAGAG TAATGTAAAT 
AGTCCGGGTG AGGATGCAGC GGCGGCGGCA GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC 
ACGACGACGG CGGGAAGTCC CGGAGCGGGT AGCGCCGGGC GACAGACCGC CGTTCTCGTG 
GAGAGCGACG GGTTCGTGAC GAAGAACGGC CACAAGGTGG TCATCCACTC GCGATCGCCG 
AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188621.1
CDS	269..6538
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGERTNQISWIWSEEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201692.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant Z, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201692.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG ACATCCTTAT CCTTACCTGG TTCACCGTTT 
AACATACGCA GGGGATCACG TAGTTCTCAC AAGTACACGA TACGGAACGG ACGTGGCCGC 
TTTGGTATAC CCGGTAGCGA TCGTAAGCCA TTGGTATTGT CAACATATCA GGATGCCCAG 
CAGCACTTGC CCTATGCCGA CGACTCGAAT GCCGTCACCC CGATGTCCGA AGAGAATGGG 
GCCATCATAG TGCCCGTGTA CTATGGCAAT CTAGGCTCCC GACACTCATC GTATACCTCG 
CATCAGTCCC GAATATCGTA TACCTCACAT GGCGATCTAC TCGGCGGCAT GGCCGTCATG 
GGCGTCAGCA CAATGACCAA GGAGAGCAAA TTGCGCAACC GGAACACACG CAATCAATCA 
GTGGGCGCCA CCAATGGCGG CACCACCTGT CTGGACACCA ATCACAAGCT CGATCATCGC 
GACTACGAAA TTGGCCTGGA GTGCACGGAC GAAGCTGGCA AGATTAAACA TCATGACAAT 
CCTTTTATCG AGCCCGTCCA GACACAAACG GTGGTTGATA TGAAAGATGT GATGGTCCTG 
AATGACATCA TCGAACAGGC CGCTGGTCGG CACAGTCGGG CAAGCGATCG CGGTGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TTTTCAAGCT GGCCAAATCG TGGCCGACGC TGAATTTGCT CATATCGATT 
ATGGGCCGCA CTGTCGGCGC CCTCGGCAAT CTGACCTTCG TCCTGTGCAT TATTATCTTC 
ATATTCGCGG TCATGGGCAT GCAGCTGTTT GGCAAGAACT ACACAGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGG TGAGAGGACC AACCAGATCA GTTGGATTTG GAGCGAAGAG 
CATGGTGACA ACGAACTGGA GCTGGGCCAC GACGAGATCC TCGCCGACGG CCTCATCAAG 
AAGGGGATCA AGGAGCAGAC GCAACTGGAG GTGGCCATCG GGGATGGCAT GGAATTCACG 
ATACACGGCG ACATGAAGAA CAACAAGCCG AAGAAATCCA AATATCTAAA TAACGCAACG 
GACGACGACA CTGCCAGCAT TAACTCATAT GGTAGCCATA AGAATCGACC ATTCAAGGAC 
GAGAGCCACA AGGGCAGCGC CGAGACGATG GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG 
GAGGATTTAG GTCTCGACGA GGAACTGGAC GAGGAGGGCG AATGCGAGGA GGGCCCGCTC 
GACGGTGATA TCATTATTCA TGCACACGAC GAGGATATAC TCGATGAATA TCCAGCTGAT 
TGCTGCCCCG ATTCGTACTA TAAGAAATTT CCGATCTTAG CCGGTGACGA TGACTCGCCG 
TTCTGGCAAG GATGGGGCAA TTTACGACTG AAAACTTTTC AATTAATTGA AAATAAATAT 
TTTGAAACAG CTGTTATCAC TATGATTTTA ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT 
GTACATCTGC CACAAAGACC CATACTGCAG GATATTTTAT ACTATATGGA CAGAATATTT 
ACGGTTATAT TCTTCTTGGA AATGTTAATC AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC 
TTCACCAACG CGTGGTGTTG GCTCGATTTC GTGATTGTCA TGGTATCGCT TATCAACTTC 
GTTGCTTCAC TTGTTGGAGC TGGTGGTATT CAAGCCTTCA AGACTATGCG AACGTTAAGA 
GCACTGAGAC CACTACGTGC CATGTCCCGT ATGCAGGGCA TGAGGGTCGT CGTTAATGCG 
CTGGTACAAG CTATACCGTC CATCTTCAAT GTGCTATTGG TGTGTCTAAT ATTTTGGCTA 
ATTTTTGCCA TAATGGGTGT ACAGCTTTTT GCTGGAAAAT ATTTTAAGTG CGAGGACATG 
AATGGCACGA AGCTCAGCCA CGAGATCATA CCAAATCGCA ATGCCTGCGA GAGCGAGAAC 
TACACGTGGG TGAATTCAGC AATGAATTTC GATCATGTAG GTAACGCGTA TCTGTGCCTT 
TTCCAAGTGG CCACCTTCAA AGGCTGGATA CAAATCATGA ACGATGCTAT CGATTCACGA 
GAGGTGGACA AGCAACCAAT TCGTGAAACG AACATCTACA TGTATTTATA TTTCGTATTC 
TTCATCATAT TTGGATCATT TTTCACACTC AATCTGTTCA TTGGTGTTAT CATTGATAAT 
TTTAATGAGC AAAAGAAAAA AGCAGGTGGA TCATTAGAAA TGTTCATGAC AGAAGATCAG 
AAAAAGTACT ATAATGCTAT GAAAAAGATG GGCTCTAAAA AACCATTAAA AGCCATTCCA 
AGACCAAGGT GGCGACCACA AGCAATAGTC TTTGAAATAG TAACCGATAA GAAATTCGAT 
ATAATCATTA TGTTATTCAT TGGTCTGAAC ATGTTCACCA TGACCCTCGA TCGTTACGAT 
GCGTCGGACA CGTATAACGC GGTCCTAGAC TATCTCAATG CGATATTCGT AGTTATTTTC 
AGTTCCGAAT GTCTATTAAA AATATTCGCT TTACGATATC ACTATTTTAT TGAGCCATGG 
AATTTATTTG ATGTAGTAGT TGTCATTTTA TCCATCTTAG GTCTTGTACT TAGCGATATT 
ATCGAGAAGT ACTTCGTGTC GCCGACCCTG CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC 
CGTGTCCTTC GACTGGTGAA GGGAGCCAAG GGCATTCGGA CACTGCTCTT CGCGTTGGCC 
ATGTCGCTGC CGGCCCTGTT CAACATCTGC CTGCTGCTGT TCCTGGTCAT GTTCATCTTT 
GCCATTTTCG GCATGTCGTT CTTCATGCAC GTGAAGGAGA AGAGCGGCAT CAACGACGTC 
TACAACTTCA AGACCTTTGG CCAGAGCATG ATCCTGCTCT TTCAGATGTC GACGTCAGCC 
GGTTGGGATG GTGTACTGGA CGCCATTATC AATGAGGAAG CATGCGATCC ACCCGACAAC 
GACAAAGGCT ATCCGGGCAA TTGTGGTTCA GCGACCGTTG GAATAACGTT TCTCCTCTCA 
TACCTAGTTA TAAGCTTTTT GATAGTTATT AATATGTACA TTGCTGTCAT TCTCGAGAAC 
TATAGTCAGG CCACCGAGGA CGTGCAAGAG GGTCTAACCG ACGACGACTA CGACATGTAC 
TATGAGATCT GGCAGCAATT CGATCCGGAG GGCACCCAGT ACATACGCTA TGATCAGCTG 
TCCGAATTCC TGGACGTACT GGAGCCCCCG CTGCAGATCC ACAAACCGAA CAAGTACAAG 
ATCATATCGA TGGACATACC CATCTGTCGC GGTGACCTCA TGTACTGCGT CGACATCCTC 
GACGCCCTTA CGAAAGACTT CTTTGCGCGG AAGGGCAATC CGATAGAGGA GACGGGTGAG 
ATTGGTGAGA TAGCGGCCCG CCCGGATACG GAGGGCTACG AGCCCGTCTC ATCAACGCTG 
TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG CTAATCCAGC ACGCCTGGCG AAAGCACAAG 
GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG CCGGATACGG ATCATGGCGA TGGCGGTGAT 
CCGGATGCCG GGGACCCGGC GCCCGATGAA GCAACGGACG GCGATGCGCC CGCTGGTGGA 
GATGGTAGTG TTAACGGTAC TGCAGAAGGA GCTGCCGATG CCGATGAGAG TAATGTAAAT 
AGTCCGGGTG AGGATGCAGC GGCGGCGGCA GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC 
ACGACGACGG CGGGAAGTCC CGGAGCGGGT AGCGCCGGGC GACAGACCGC CGTTCTCGTG 
GAGAGCGACG GGTTCGTGAC GAAGAACGGC CACAAGGTGG TCATCCACTC GCGATCGCCG 
AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201675.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188604.1 Drosophila melanogaster paralytic (para), transcript variant I, mRNA.

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CloneID	OFa03670
Clone ID Related Accession (Same CDS sequence)	NM_001201675.1
Accession Version	NM_001201675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGAGGACG ATGACGAGGA TGGGCCGACG TTCAAAGACA AGGCACTCGA AGTGATCCTC 
AAAGGCATCG ATGTGTTTTG TGTGTGGGAC TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG 
TGGGTATCGC TCATCGTCTT CGATCCCTTC GTCGAGCTCT TCATCACGCT GTGCATTGTG 
GTCAACACGA TGTTCATGGC AATGGATCAC CACGATATGA ACAAGGAGAT GGAACGCGTG 
CTCAAGAGTG GCAACTATTT CTTCACCGCC ACCTTTGCCA TCGAGGCCAC CATGAAGCTA 
ATGGCCATGA GCCCCAAGTA CTATTTCCAG GAGGGCTGGA ACATCTTCGA CTTCATTATC 
GTGGCCCTAT CGCTATTGGA ACTGGGACTC GAGGGTGTCC AGGGTCTGTC CGTATTGCGT 
TCCTTTCGAT TGCTGCGTGT ATTCAAACTG GCCAAGTCTT GGCCCACACT TAATTTACTC 
ATTTCGATTA TGGGACGCAC CATGGGCGCT TTGGGTAATC TGACATTTGT ACTTTGCATT 
ATCATCTTCA TCTTTGCGGT GATGGGAATG CAACTGTTCG GAAAGAATTA TCATGATCAC 
AAGGACCGCT TTCCGGATGG CGACCTGCCG CGCTGGAACT TCACCGACTT TATGCACAGC 
TTCATGATCG TGTTCCGGGT GCTCTGCGGA GAATGGATCG AGTCCATGTG GGACTGCATG 
TACGTGGGCG ATGTCTCGTG CATTCCCTTC TTCTTGGCCA CCGTTGTCAT CGGCAATCTT 
GTGGTACTTA ACCTTTTCTT AGCCTTGCTT TTGTCCAATT TTGGCTCATC TAGCTTATCA 
GCGCCGACTG CCGATAACGA TACGAATAAA ATAGCCGAGG CCTTCAATCG AATTGGCCGA 
TTTAAAAGTT GGGTTAAGCG TAATATTGCT GATTGTTTCA AGTTAATACG TAACAAATTG 
ACAAATCAAA TAAGTGATCA ACCATCAGAG CATGGTGACA ACGAACTGGA GCTGGGCCAC 
GACGAGATCC TCGCCGACGG CCTCATCAAG AAGGGGATCA AGGAGCAGAC GCAACTGGAG 
GTGGCCATCG GGGATGGCAT GGAATTCACG ATACACGGCG ACATGAAGAA CAACAAGCCG 
AAGAAATCCA AATATCTAAA TAACGCAACG GACGACGACA CTGCCAGCAT TAACTCATAT 
GGTAGCCATA AGAATCGACC ATTCAAGGAC GAGAGCCACA AGGGCAGCGC CGAGACGATG 
GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG GAGGATTTAG GTCTCGACGA GGAACTGGAC 
GAGGAGGGCG AATGCGAGGA GGGCCCGCTC GACGGTGATA TCATTATTCA TGCACACGAC 
GAGGATATAC TCGATGAATA TCCAGCTGAT TGCTGCCCCG ATTCGTACTA TAAGAAATTT 
CCGATCTTAG CCGGTGACGA TGACTCGCCG TTCTGGCAAG GATGGGGCAA TTTACGACTG 
AAAACTTTTC AATTAATTGA AAATAAATAT TTTGAAACAG CTGTTATCAC TATGATTTTA 
ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT GTACATCTGC CACAAAGACC CATACTGCAG 
GATATTTTAT ACTATATGGA CAGAATATTT ACGGTTATAT TCTTCTTGGA AATGTTAATC 
AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC TTCACCAACG CGTGGTGTTG GCTCGATTTC 
GTGATTGTCA TGGTATCGCT TATCAACTTC GTTGCTTCAC TTGTTGGAGC TGGTGGTATT 
CAAGCCTTCA AGACTATGCG AACGTTAAGA GCACTGAGAC CACTACGTGC CATGTCCCGT 
ATGCAGGGCA TGAGGGTCGT CGTTAATGCG CTGGTACAAG CTATACCGTC CATCTTCAAT 
GTGCTATTGG TGTGTCTAAT ATTTTGGCTA ATTTTTGCCA TAATGGGTGT ACAGCTTTTT 
GCTGGAAAAT ATTTTAAGTG CGAGGACATG AATGGCACGA AGCTCAGCCA CGAGATCATA 
CCAAATCGCA ATGCCTGCGA GAGCGAGAAC TACACGTGGG TGAATTCAGC AATGAATTTC 
GATCATGTAG GTAACGCGTA TCTGTGCCTT TTCCAAGTGG CCACCTTCAA AGGCTGGATA 
CAAATCATGA ACGATGCTAT CGATTCACGA GAGGTGGACA AGCAACCAAT TCGTGAAACG 
AACATCTACA TGTATTTATA TTTCGTATTC TTCATCATAT TTGGATCATT TTTCACACTC 
AATCTGTTCA TTGGTGTTAT CATTGATAAT TTTAATGAGC AAAAGAAAAA AGCAGGTGGA 
TCATTAGAAA TGTTCATGAC AGAAGATCAG AAAAAGTACT ATAATGCTAT GAAAAAGATG 
GGCTCTAAAA AACCATTAAA AGCCATTCCA AGACCAAGGT GGCGACCACA AGCAATAGTC 
TTTGAAATAG TAACCGATAA GAAATTCGAT ATAATCATTA TGTTATTCAT TGGTCTGAAC 
ATGTTCACCA TGACCCTCGA TCGTTACGAT GCGTCGGACA CGTATAACGC GGTCCTAGAC 
TATCTCAATG CGATATTCGT AGTTATTTTC AGTTCCGAAT GTCTATTAAA AATATTCGCT 
TTACGATATC ACTATTTTAT TGAGCCATGG AATTTATTTG ATGTAGTAGT TGTCATTTTA 
TCCATCTTAG GTCTTGTACT TAGCGATATT ATCGAGAAGT ACTTCGTGTC GCCGACCCTG 
CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC CGTGTCCTTC GACTGGTGAA GGGAGCCAAG 
GGCATTCGGA CACTGCTCTT CGCGTTGGCC ATGTCGCTGC CGGCCCTGTT CAACATCTGC 
CTGCTGCTGT TCCTGGTCAT GTTCATCTTT GCCATTTTCG GCATGTCGTT CTTCATGCAC 
GTGAAGGAGA AGAGCGGCAT CAACGACGTC TACAACTTCA AGACCTTTGG CCAGAGCATG 
ATCCTGCTCT TTCAGATGTC GACGTCAGCC GGTTGGGATG GTGTACTGGA CGCCATTATC 
AATGAGGAAG CATGCGATCC ACCCGACAAC GACAAAGGCT ATCCGGGCAA TTGTGGTTCA 
GCGACCGTTG GAATAACGTT TCTCCTCTCA TACCTAGTTA TAAGCTTTTT GATAGTTATT 
AATATGTACA TTGCTGTCAT TCTCGAGAAC TATAGTCAGG CCACCGAGGA CGTGCAAGAG 
GGTCTAACCG ACGACGACTA CGACATGTAC TATGAGATCT GGCAGCAATT CGATCCGGAG 
GGCACCCAGT ACATACGCTA TGATCAGCTG TCCGAATTCC TGGACGTACT GGAGCCCCCG 
CTGCAGATCC ACAAACCGAA CAAGTACAAG ATCATATCGA TGGACATACC CATCTGTCGC 
GGTGACCTCA TGTACTGCGT CGACATCCTC GACGCCCTTA CGAAAGACTT CTTTGCGCGG 
AAGGGCAATC CGATAGAGGA GACGGGTGAG ATTGGTGAGA TAGCGGCCCG CCCGGATACG 
GAGGGCTACG AGCCCGTCTC ATCAACGCTG TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG 
CTAATCCAGC ACGCCTGGCG AAAGCACAAG GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG 
CCGGATACGG ATCATGGCGA TGGCGGTGAT CCGGATGCCG GGGACCCGGC GCCCGATGAA 
GCAACGGACG GCGATGCGCC CGCTGGTGGA GATGGTAGTG TTAACGGTAC TGCAGAAGGA 
GCTGCCGATG CCGATGAGAG TAATGTAAAT AGTCCGGGTG AGGATGCAGC GGCGGCGGCA 
GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC ACGACGACGG CGGGAAGTCC CGGAGCGGGT 
AGCGCCGGGC GACAGACCGC CGTTCTCGTG GAGAGCGACG GGTTCGTGAC GAAGAACGGC 
CACAAGGTGG TCATCCACTC GCGATCGCCG AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188604.1
CDS	269..6508
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201675.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201675.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGAGGACG ATGACGAGGA TGGGCCGACG TTCAAAGACA AGGCACTCGA AGTGATCCTC 
AAAGGCATCG ATGTGTTTTG TGTGTGGGAC TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG 
TGGGTATCGC TCATCGTCTT CGATCCCTTC GTCGAGCTCT TCATCACGCT GTGCATTGTG 
GTCAACACGA TGTTCATGGC AATGGATCAC CACGATATGA ACAAGGAGAT GGAACGCGTG 
CTCAAGAGTG GCAACTATTT CTTCACCGCC ACCTTTGCCA TCGAGGCCAC CATGAAGCTA 
ATGGCCATGA GCCCCAAGTA CTATTTCCAG GAGGGCTGGA ACATCTTCGA CTTCATTATC 
GTGGCCCTAT CGCTATTGGA ACTGGGACTC GAGGGTGTCC AGGGTCTGTC CGTATTGCGT 
TCCTTTCGAT TGCTGCGTGT ATTCAAACTG GCCAAGTCTT GGCCCACACT TAATTTACTC 
ATTTCGATTA TGGGACGCAC CATGGGCGCT TTGGGTAATC TGACATTTGT ACTTTGCATT 
ATCATCTTCA TCTTTGCGGT GATGGGAATG CAACTGTTCG GAAAGAATTA TCATGATCAC 
AAGGACCGCT TTCCGGATGG CGACCTGCCG CGCTGGAACT TCACCGACTT TATGCACAGC 
TTCATGATCG TGTTCCGGGT GCTCTGCGGA GAATGGATCG AGTCCATGTG GGACTGCATG 
TACGTGGGCG ATGTCTCGTG CATTCCCTTC TTCTTGGCCA CCGTTGTCAT CGGCAATCTT 
GTGGTACTTA ACCTTTTCTT AGCCTTGCTT TTGTCCAATT TTGGCTCATC TAGCTTATCA 
GCGCCGACTG CCGATAACGA TACGAATAAA ATAGCCGAGG CCTTCAATCG AATTGGCCGA 
TTTAAAAGTT GGGTTAAGCG TAATATTGCT GATTGTTTCA AGTTAATACG TAACAAATTG 
ACAAATCAAA TAAGTGATCA ACCATCAGAG CATGGTGACA ACGAACTGGA GCTGGGCCAC 
GACGAGATCC TCGCCGACGG CCTCATCAAG AAGGGGATCA AGGAGCAGAC GCAACTGGAG 
GTGGCCATCG GGGATGGCAT GGAATTCACG ATACACGGCG ACATGAAGAA CAACAAGCCG 
AAGAAATCCA AATATCTAAA TAACGCAACG GACGACGACA CTGCCAGCAT TAACTCATAT 
GGTAGCCATA AGAATCGACC ATTCAAGGAC GAGAGCCACA AGGGCAGCGC CGAGACGATG 
GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG GAGGATTTAG GTCTCGACGA GGAACTGGAC 
GAGGAGGGCG AATGCGAGGA GGGCCCGCTC GACGGTGATA TCATTATTCA TGCACACGAC 
GAGGATATAC TCGATGAATA TCCAGCTGAT TGCTGCCCCG ATTCGTACTA TAAGAAATTT 
CCGATCTTAG CCGGTGACGA TGACTCGCCG TTCTGGCAAG GATGGGGCAA TTTACGACTG 
AAAACTTTTC AATTAATTGA AAATAAATAT TTTGAAACAG CTGTTATCAC TATGATTTTA 
ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT GTACATCTGC CACAAAGACC CATACTGCAG 
GATATTTTAT ACTATATGGA CAGAATATTT ACGGTTATAT TCTTCTTGGA AATGTTAATC 
AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC TTCACCAACG CGTGGTGTTG GCTCGATTTC 
GTGATTGTCA TGGTATCGCT TATCAACTTC GTTGCTTCAC TTGTTGGAGC TGGTGGTATT 
CAAGCCTTCA AGACTATGCG AACGTTAAGA GCACTGAGAC CACTACGTGC CATGTCCCGT 
ATGCAGGGCA TGAGGGTCGT CGTTAATGCG CTGGTACAAG CTATACCGTC CATCTTCAAT 
GTGCTATTGG TGTGTCTAAT ATTTTGGCTA ATTTTTGCCA TAATGGGTGT ACAGCTTTTT 
GCTGGAAAAT ATTTTAAGTG CGAGGACATG AATGGCACGA AGCTCAGCCA CGAGATCATA 
CCAAATCGCA ATGCCTGCGA GAGCGAGAAC TACACGTGGG TGAATTCAGC AATGAATTTC 
GATCATGTAG GTAACGCGTA TCTGTGCCTT TTCCAAGTGG CCACCTTCAA AGGCTGGATA 
CAAATCATGA ACGATGCTAT CGATTCACGA GAGGTGGACA AGCAACCAAT TCGTGAAACG 
AACATCTACA TGTATTTATA TTTCGTATTC TTCATCATAT TTGGATCATT TTTCACACTC 
AATCTGTTCA TTGGTGTTAT CATTGATAAT TTTAATGAGC AAAAGAAAAA AGCAGGTGGA 
TCATTAGAAA TGTTCATGAC AGAAGATCAG AAAAAGTACT ATAATGCTAT GAAAAAGATG 
GGCTCTAAAA AACCATTAAA AGCCATTCCA AGACCAAGGT GGCGACCACA AGCAATAGTC 
TTTGAAATAG TAACCGATAA GAAATTCGAT ATAATCATTA TGTTATTCAT TGGTCTGAAC 
ATGTTCACCA TGACCCTCGA TCGTTACGAT GCGTCGGACA CGTATAACGC GGTCCTAGAC 
TATCTCAATG CGATATTCGT AGTTATTTTC AGTTCCGAAT GTCTATTAAA AATATTCGCT 
TTACGATATC ACTATTTTAT TGAGCCATGG AATTTATTTG ATGTAGTAGT TGTCATTTTA 
TCCATCTTAG GTCTTGTACT TAGCGATATT ATCGAGAAGT ACTTCGTGTC GCCGACCCTG 
CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC CGTGTCCTTC GACTGGTGAA GGGAGCCAAG 
GGCATTCGGA CACTGCTCTT CGCGTTGGCC ATGTCGCTGC CGGCCCTGTT CAACATCTGC 
CTGCTGCTGT TCCTGGTCAT GTTCATCTTT GCCATTTTCG GCATGTCGTT CTTCATGCAC 
GTGAAGGAGA AGAGCGGCAT CAACGACGTC TACAACTTCA AGACCTTTGG CCAGAGCATG 
ATCCTGCTCT TTCAGATGTC GACGTCAGCC GGTTGGGATG GTGTACTGGA CGCCATTATC 
AATGAGGAAG CATGCGATCC ACCCGACAAC GACAAAGGCT ATCCGGGCAA TTGTGGTTCA 
GCGACCGTTG GAATAACGTT TCTCCTCTCA TACCTAGTTA TAAGCTTTTT GATAGTTATT 
AATATGTACA TTGCTGTCAT TCTCGAGAAC TATAGTCAGG CCACCGAGGA CGTGCAAGAG 
GGTCTAACCG ACGACGACTA CGACATGTAC TATGAGATCT GGCAGCAATT CGATCCGGAG 
GGCACCCAGT ACATACGCTA TGATCAGCTG TCCGAATTCC TGGACGTACT GGAGCCCCCG 
CTGCAGATCC ACAAACCGAA CAAGTACAAG ATCATATCGA TGGACATACC CATCTGTCGC 
GGTGACCTCA TGTACTGCGT CGACATCCTC GACGCCCTTA CGAAAGACTT CTTTGCGCGG 
AAGGGCAATC CGATAGAGGA GACGGGTGAG ATTGGTGAGA TAGCGGCCCG CCCGGATACG 
GAGGGCTACG AGCCCGTCTC ATCAACGCTG TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG 
CTAATCCAGC ACGCCTGGCG AAAGCACAAG GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG 
CCGGATACGG ATCATGGCGA TGGCGGTGAT CCGGATGCCG GGGACCCGGC GCCCGATGAA 
GCAACGGACG GCGATGCGCC CGCTGGTGGA GATGGTAGTG TTAACGGTAC TGCAGAAGGA 
GCTGCCGATG CCGATGAGAG TAATGTAAAT AGTCCGGGTG AGGATGCAGC GGCGGCGGCA 
GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC ACGACGACGG CGGGAAGTCC CGGAGCGGGT 
AGCGCCGGGC GACAGACCGC CGTTCTCGTG GAGAGCGACG GGTTCGTGAC GAAGAACGGC 
CACAAGGTGG TCATCCACTC GCGATCGCCG AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001201709.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188638.1 Drosophila melanogaster paralytic (para), transcript variant AQ, mRNA.

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CloneID	OFa03671
Clone ID Related Accession (Same CDS sequence)	NM_001201709.1
Accession Version	NM_001201709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform AQ
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGAGGACG ATGACGAGGA TGGGCCGACG TTCAAAGACA AGGCACTCGA AGTGATCCTC 
AAAGGCATCG ATGTGTTTTG TGTGTGGGAC TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG 
TGGGTATCGC TCATCGTCTT CGATCCCTTC GTCGAGCTCT TCATCACGCT GTGCATTGTG 
GTCAACACGA TGTTCATGGC AATGGATCAC CACGATATGA ACAAGGAGAT GGAACGCGTG 
CTCAAGAGTG GCAACTATTT CTTCACCGCC ACCTTTGCCA TCGAGGCCAC CATGAAGCTA 
ATGGCCATGA GCCCCAAGTA CTATTTCCAG GAGGGCTGGA ACATCTTCGA CTTCATTATC 
GTGGCCCTAT CGCTATTGGA ACTGGGACTC GAGGGTGTCC AGGGTCTGTC CGTATTGCGT 
TCCTTTCGAT TGCTGCGTGT ATTCAAACTG GCCAAGTCTT GGCCCACACT TAATTTACTC 
ATTTCGATTA TGGGACGCAC CATGGGCGCT TTGGGTAATC TGACATTTGT ACTTTGCATT 
ATCATCTTCA TCTTTGCGGT GATGGGAATG CAACTGTTCG GAAAGAATTA TCATGATCAC 
AAGGACCGCT TTCCGGATGG CGACCTGCCG CGCTGGAACT TCACCGACTT TATGCACAGC 
TTCATGATCG TGTTCCGGGT GCTCTGCGGA GAATGGATCG AGTCCATGTG GGACTGCATG 
TACGTGGGCG ATGTCTCGTG CATTCCCTTC TTCTTGGCCA CCGTTGTCAT CGGCAATCTT 
GTGGTACTTA ACCTTTTCTT AGCCTTGCTT TTGTCCAATT TTGGCTCATC TAGCTTATCA 
GCGCCGACTG CCGATAACGA TACGAATAAA ATAGCCGAGG CCTTCAATCG AATTGGCCGA 
TTTAAAAGTT GGGTTAAGCG TAATATTGCT GATTGTTTCA AGTTAATACG TAACAAATTG 
ACAAATCAAA TAAGTGATCA ACCATCAGGC AAAGGCGTTT GTCGTTGTAT ATCTGCAGAG 
CATGGTGACA ACGAACTGGA GCTGGGCCAC GACGAGATCC TCGCCGACGG CCTCATCAAG 
AAGGGGATCA AGGAGCAGAC GCAACTGGAG GTGGCCATCG GGGATGGCAT GGAATTCACG 
ATACACGGCG ACATGAAGAA CAACAAGCCG AAGAAATCCA AATATCTAAA TAACGCAACG 
GACGACGACA CTGCCAGCAT TAACTCATAT GGTAGCCATA AGAATCGACC ATTCAAGGAC 
GAGAGCCACA AGGGCAGCGC CGAGACGATG GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG 
GAGGATTTAG GTCTCGACGA GGAACTGGAC GAGGAGGGCG AATGCGAGGA GGGCCCGCTC 
GACGGTGATA TCATTATTCA TGCACACGAC GAGGATATAC TCGATGAATA TCCAGCTGAT 
TGCTGCCCCG ATTCGTACTA TAAGAAATTT CCGATCTTAG CCGGTGACGA TGACTCGCCG 
TTCTGGCAAG GATGGGGCAA TTTACGACTG AAAACTTTTC AATTAATTGA AAATAAATAT 
TTTGAAACAG CTGTTATCAC TATGATTTTA ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT 
GTACATCTGC CACAAAGACC CATACTGCAG GATATTTTAT ACTATATGGA CAGAATATTT 
ACGGTTATAT TCTTCTTGGA AATGTTAATC AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC 
TTCACCAACG CGTGGTGTTG GCTCGATTTC GTGATTGTCA TGGTATCGCT TATCAACTTC 
GTTGCTTCAC TTGTTGGAGC TGGTGGTATT CAAGCCTTCA AGACTATGCG AACGTTAAGA 
GCACTGAGAC CACTACGTGC CATGTCCCGT ATGCAGGGCA TGAGGGTCGT CGTTAATGCG 
CTGGTACAAG CTATACCGTC CATCTTCAAT GTGCTATTGG TGTGTCTAAT ATTTTGGCTA 
ATTTTTGCCA TAATGGGTGT ACAGCTTTTT GCTGGAAAAT ATTTTAAGTG CGAGGACATG 
AATGGCACGA AGCTCAGCCA CGAGATCATA CCAAATCGCA ATGCCTGCGA GAGCGAGAAC 
TACACGTGGG TGAATTCAGC AATGAATTTC GATCATGTAG GTAACGCGTA TCTGTGCCTT 
TTCCAAGTGG CCACCTTCAA AGGCTGGATA CAAATCATGA ACGATGCTAT CGATTCACGA 
GAGGTGGACA AGCAACCAAT TCGTGAAACG AACATCTACA TGTATTTATA TTTCGTATTC 
TTCATCATAT TTGGATCATT TTTCACACTC AATCTGTTCA TTGGTGTTAT CATTGATAAT 
TTTAATGAGC AAAAGAAAAA AGCAGGTGGA TCATTAGAAA TGTTCATGAC AGAAGATCAG 
AAAAAGTACT ATAATGCTAT GAAAAAGATG GGCTCTAAAA AACCATTAAA AGCCATTCCA 
AGACCAAGGT GGCGACCACA AGCAATAGTC TTTGAAATAG TAACCGATAA GAAATTCGAT 
ATAATCATTA TGTTATTCAT TGGTCTGAAC ATGTTCACCA TGACCCTCGA TCGTTACGAT 
GCGTCGGACA CGTATAACGC GGTCCTAGAC TATCTCAATG CGATATTCGT AGTTATTTTC 
AGTTCCGAAT GTCTATTAAA AATATTCGCT TTACGATATC ACTATTTTAT TGAGCCATGG 
AATTTATTTG ATGTAGTAGT TGTCATTTTA TCCATCTTAG GTCTTGTACT TAGCGATATT 
ATCGAGAAGT ACTTCGTGTC GCCGACCCTG CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC 
CGTGTCCTTC GACTGGTGAA GGGAGCCAAG GGCATTCGGA CACTGCTCTT CGCGTTGGCC 
ATGTCGCTGC CGGCCCTGTT CAACATCTGC CTGCTGCTGT TCCTGGTCAT GTTCATCTTT 
GCCATTTTCG GCATGTCGTT CTTCATGCAC GTGAAGGAGA AGAGCGGCAT CAACGACGTC 
TACAACTTCA AGACCTTTGG CCAGAGCATG ATCCTGCTCT TTCAGATGTC GACGTCAGCC 
GGTTGGGATG GTGTACTGGA CGCCATTATC AATGAGGAAG CATGCGATCC ACCCGACAAC 
GACAAAGGCT ATCCGGGCAA TTGTGGTTCA GCGACCGTTG GAATAACGTT TCTCCTCTCA 
TACCTAGTTA TAAGCTTTTT GATAGTTATT AATATGTACA TTGCTGTCAT TCTCGAGAAC 
TATAGTCAGG CCACCGAGGA CGTGCAAGAG GGTCTAACCG ACGACGACTA CGACATGTAC 
TATGAGATCT GGCAGCAATT CGATCCGGAG GGCACCCAGT ACATACGCTA TGATCAGCTG 
TCCGAATTCC TGGACGTACT GGAGCCCCCG CTGCAGATCC ACAAACCGAA CAAGTACAAG 
ATCATATCGA TGGACATACC CATCTGTCGC GGTGACCTCA TGTACTGCGT CGACATCCTC 
GACGCCCTTA CGAAAGACTT CTTTGCGCGG AAGGGCAATC CGATAGAGGA GACGGGTGAG 
ATTGGTGAGA TAGCGGCCCG CCCGGATACG GAGGGCTACG AGCCCGTCTC ATCAACGCTG 
TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG CTAATCCAGC ACGCCTGGCG AAAGCACAAG 
GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG CCGGATACGG ATCATGGCGA TGGCGGTGAT 
CCGGATGCCG GGGACCCGGC GCCCGATGAA GCAACGGACG GCGATGCGCC CGCTGGTGGA 
GATGGTAGTG TTAACGGTAC TGCAGAAGGA GCTGCCGATG CCGATGAGAG TAATGTAAAT 
AGTCCGGGTG AGGATGCAGC GGCGGCGGCA GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC 
ACGACGACGG CGGGAAGTCC CGGAGCGGGT AGCGCCGGGC GACAGACCGC CGTTCTCGTG 
GAGAGCGACG GGTTCGTGAC GAAGAACGGC CACAAGGTGG TCATCCACTC GCGATCGCCG 
AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188638.1
CDS	269..6538
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEVPQYGRKKKQKEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSGKGVCRCISAEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVSLINFVASLVGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001201709.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant AQ, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201709.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGGTG CCGCAATATG GTCGCAAGAA AAAACAAAAA 
GAAATCCGAT ATGATGACGA GGACGAGGAT GAAGGTCCAC AACCGGATCC TACACTTGAA 
CAGGGTGTGC CAATACCTGT TCGATTGCAG GGCAGCTTCC CGCCGGAATT GGCCTCCACT 
CCTCTCGAGG ATATCGATCC CTACTACAGC AATGTACTGA CATTCGTAGT TGTAAGCAAA 
GGAAAAGATA TTTTTCGCTT TTCTGCATCA AAAGCAATGT GGATGCTCGA TCCATTCAAT 
CCGATACGTC GTGTGGCCAT TTACATTCTA GTGCATCCAT TATTTTCCCT ATTCATCATC 
ACCACAATTC TCGTCAACTG CATCCTGATG ATAATGCCGA CAACGCCCAC GGTTGAGTCC 
ACTGAGGTGA TATTCACCGG AATCTACACA TTTGAATCAG CTGTTAAAGT GATGGCACGA 
GGTTTCATTT TATGCCCGTT TACGTATCTT AGAGATGCAT GGAATTGGCT GGACTTCGTA 
GTAATAGCTT TAGCTTATGT GACCATGGGT ATAGATTTAG GTAATCTAGC AGCCCTGCGA 
ACGTTTAGGG TGCTGCGAGC GCTTAAAACC GTAGCCATTG TGCCAGGCTT GAAGACCATC 
GTCGGCGCCG TCATCGAATC GGTGAAGAAT CTGCGCGATG TGATTATCCT GACCATGTTC 
TCCCTGTCGG TGTTCGCGTT GATGGGCCTA CAGATCTATA TGGGCGTGCT CACGCAGAAG 
TGCATCAAGA AGTTCCCGCT GGACGGTTCC TGGGGCAATC TGACCGACGA GAACTGGGAC 
TATCACAATC GCAATAGCTC CAATTGGTAT TCCGAGGACG AGGGCATCTC ATTTCCGTTA 
TGCGGCAATA TATCCGGTGC GGGGCAATGC GACGACGATT ACGTGTGCCT GCAGGGGTTT 
GGTCCGAATC CGAATTATGG CTACACCAGC TTCGATTCGT TCGGATGGGC TTTCCTGTCC 
GCCTTCCGGC TGATGACACA GGACTTCTGG GAGGATCTGT ACCAGCTGGT GTTGCGCGCC 
GCCGGACCAT GGCACATGCT GTTCTTTATA GTCATCATCT TCCTAGGTTC ATTCTATCTT 
GTGAATTTGA TTTTGGCCAT TGTTGCCATG TCGTATGACG AATTGCAAAA GAAGGCCGAA 
GAAGAAGAGG CTGCCGAAGA GGAGGCGATA CGTGAAGCGG AAGAAGCTGC CGCCGCCAAA 
GCGGCCAAGC TGGAGGAGCG GGCCAATGCG CAGGCTCAGG CAGCAGCGGA TGCGGCTGCC 
GCCGAAGAGG CTGCACTGCA TCCGGAAATG GCCAAGAGTC CGACGTATTC TTGCATCAGC 
TATGAGCTAT TTGTTGGCGG CGAGAAGGGC AACGATGACA ACAACAAAGA GAAGATGTCC 
ATTCGGAGCG TCGAGGTGGA GTCGGAGTCG GTGAGCGTTA TACAAAGACA ACCAGCACCT 
ACCACAGCAC ACCAAGCTAC CAAAGTTCGT AAAGTGAGCA CGTACACGAT ACGGAACGGA 
CGTGGCCGCT TTGGTATACC CGGTAGCGAT CGTAAGCCAT TGGTATTGTC AACATATCAG 
GATGCCCAGC AGCACTTGCC CTATGCCGAC GACTCGAATG CCGTCACCCC GATGTCCGAA 
GAGAATGGGG CCATCATAGT GCCCGTGTAC TATGGCAATC TAGGCTCCCG ACACTCATCG 
TATACCTCGC ATCAGTCCCG AATATCGTAT ACCTCACATG GCGATCTACT CGGCGGCATG 
GCCGTCATGG GCGTCAGCAC AATGACCAAG GAGAGCAAAT TGCGCAACCG GAACACACGC 
AATCAATCAG TGGGCGCCAC CAATGGCGGC ACCACCTGTC TGGACACCAA TCACAAGCTC 
GATCATCGCG ACTACGAAAT TGGCCTGGAG TGCACGGACG AAGCTGGCAA GATTAAACAT 
CATGACAATC CTTTTATCGA GCCCGTCCAG ACACAAACGG TGGTTGATAT GAAAGATGTG 
ATGGTCCTGA ATGACATCAT CGAACAGGCC GCTGGTCGGC ACAGTCGGGC AAGCGATCGC 
GGTGAGGACG ATGACGAGGA TGGGCCGACG TTCAAAGACA AGGCACTCGA AGTGATCCTC 
AAAGGCATCG ATGTGTTTTG TGTGTGGGAC TGTTGCTGGG TTTGGTTGAA ATTTCAGGAG 
TGGGTATCGC TCATCGTCTT CGATCCCTTC GTCGAGCTCT TCATCACGCT GTGCATTGTG 
GTCAACACGA TGTTCATGGC AATGGATCAC CACGATATGA ACAAGGAGAT GGAACGCGTG 
CTCAAGAGTG GCAACTATTT CTTCACCGCC ACCTTTGCCA TCGAGGCCAC CATGAAGCTA 
ATGGCCATGA GCCCCAAGTA CTATTTCCAG GAGGGCTGGA ACATCTTCGA CTTCATTATC 
GTGGCCCTAT CGCTATTGGA ACTGGGACTC GAGGGTGTCC AGGGTCTGTC CGTATTGCGT 
TCCTTTCGAT TGCTGCGTGT ATTCAAACTG GCCAAGTCTT GGCCCACACT TAATTTACTC 
ATTTCGATTA TGGGACGCAC CATGGGCGCT TTGGGTAATC TGACATTTGT ACTTTGCATT 
ATCATCTTCA TCTTTGCGGT GATGGGAATG CAACTGTTCG GAAAGAATTA TCATGATCAC 
AAGGACCGCT TTCCGGATGG CGACCTGCCG CGCTGGAACT TCACCGACTT TATGCACAGC 
TTCATGATCG TGTTCCGGGT GCTCTGCGGA GAATGGATCG AGTCCATGTG GGACTGCATG 
TACGTGGGCG ATGTCTCGTG CATTCCCTTC TTCTTGGCCA CCGTTGTCAT CGGCAATCTT 
GTGGTACTTA ACCTTTTCTT AGCCTTGCTT TTGTCCAATT TTGGCTCATC TAGCTTATCA 
GCGCCGACTG CCGATAACGA TACGAATAAA ATAGCCGAGG CCTTCAATCG AATTGGCCGA 
TTTAAAAGTT GGGTTAAGCG TAATATTGCT GATTGTTTCA AGTTAATACG TAACAAATTG 
ACAAATCAAA TAAGTGATCA ACCATCAGGC AAAGGCGTTT GTCGTTGTAT ATCTGCAGAG 
CATGGTGACA ACGAACTGGA GCTGGGCCAC GACGAGATCC TCGCCGACGG CCTCATCAAG 
AAGGGGATCA AGGAGCAGAC GCAACTGGAG GTGGCCATCG GGGATGGCAT GGAATTCACG 
ATACACGGCG ACATGAAGAA CAACAAGCCG AAGAAATCCA AATATCTAAA TAACGCAACG 
GACGACGACA CTGCCAGCAT TAACTCATAT GGTAGCCATA AGAATCGACC ATTCAAGGAC 
GAGAGCCACA AGGGCAGCGC CGAGACGATG GAGGGCGAGG AGAAGCGCGA CGCCAGCAAG 
GAGGATTTAG GTCTCGACGA GGAACTGGAC GAGGAGGGCG AATGCGAGGA GGGCCCGCTC 
GACGGTGATA TCATTATTCA TGCACACGAC GAGGATATAC TCGATGAATA TCCAGCTGAT 
TGCTGCCCCG ATTCGTACTA TAAGAAATTT CCGATCTTAG CCGGTGACGA TGACTCGCCG 
TTCTGGCAAG GATGGGGCAA TTTACGACTG AAAACTTTTC AATTAATTGA AAATAAATAT 
TTTGAAACAG CTGTTATCAC TATGATTTTA ATGAGTAGCT TAGCTTTGGC ATTAGAAGAT 
GTACATCTGC CACAAAGACC CATACTGCAG GATATTTTAT ACTATATGGA CAGAATATTT 
ACGGTTATAT TCTTCTTGGA AATGTTAATC AAGTGGTTGG CGCTCGGCTT CAAAGTGTAC 
TTCACCAACG CGTGGTGTTG GCTCGATTTC GTGATTGTCA TGGTATCGCT TATCAACTTC 
GTTGCTTCAC TTGTTGGAGC TGGTGGTATT CAAGCCTTCA AGACTATGCG AACGTTAAGA 
GCACTGAGAC CACTACGTGC CATGTCCCGT ATGCAGGGCA TGAGGGTCGT CGTTAATGCG 
CTGGTACAAG CTATACCGTC CATCTTCAAT GTGCTATTGG TGTGTCTAAT ATTTTGGCTA 
ATTTTTGCCA TAATGGGTGT ACAGCTTTTT GCTGGAAAAT ATTTTAAGTG CGAGGACATG 
AATGGCACGA AGCTCAGCCA CGAGATCATA CCAAATCGCA ATGCCTGCGA GAGCGAGAAC 
TACACGTGGG TGAATTCAGC AATGAATTTC GATCATGTAG GTAACGCGTA TCTGTGCCTT 
TTCCAAGTGG CCACCTTCAA AGGCTGGATA CAAATCATGA ACGATGCTAT CGATTCACGA 
GAGGTGGACA AGCAACCAAT TCGTGAAACG AACATCTACA TGTATTTATA TTTCGTATTC 
TTCATCATAT TTGGATCATT TTTCACACTC AATCTGTTCA TTGGTGTTAT CATTGATAAT 
TTTAATGAGC AAAAGAAAAA AGCAGGTGGA TCATTAGAAA TGTTCATGAC AGAAGATCAG 
AAAAAGTACT ATAATGCTAT GAAAAAGATG GGCTCTAAAA AACCATTAAA AGCCATTCCA 
AGACCAAGGT GGCGACCACA AGCAATAGTC TTTGAAATAG TAACCGATAA GAAATTCGAT 
ATAATCATTA TGTTATTCAT TGGTCTGAAC ATGTTCACCA TGACCCTCGA TCGTTACGAT 
GCGTCGGACA CGTATAACGC GGTCCTAGAC TATCTCAATG CGATATTCGT AGTTATTTTC 
AGTTCCGAAT GTCTATTAAA AATATTCGCT TTACGATATC ACTATTTTAT TGAGCCATGG 
AATTTATTTG ATGTAGTAGT TGTCATTTTA TCCATCTTAG GTCTTGTACT TAGCGATATT 
ATCGAGAAGT ACTTCGTGTC GCCGACCCTG CTCCGAGTGG TGCGTGTGGC GAAAGTGGGC 
CGTGTCCTTC GACTGGTGAA GGGAGCCAAG GGCATTCGGA CACTGCTCTT CGCGTTGGCC 
ATGTCGCTGC CGGCCCTGTT CAACATCTGC CTGCTGCTGT TCCTGGTCAT GTTCATCTTT 
GCCATTTTCG GCATGTCGTT CTTCATGCAC GTGAAGGAGA AGAGCGGCAT CAACGACGTC 
TACAACTTCA AGACCTTTGG CCAGAGCATG ATCCTGCTCT TTCAGATGTC GACGTCAGCC 
GGTTGGGATG GTGTACTGGA CGCCATTATC AATGAGGAAG CATGCGATCC ACCCGACAAC 
GACAAAGGCT ATCCGGGCAA TTGTGGTTCA GCGACCGTTG GAATAACGTT TCTCCTCTCA 
TACCTAGTTA TAAGCTTTTT GATAGTTATT AATATGTACA TTGCTGTCAT TCTCGAGAAC 
TATAGTCAGG CCACCGAGGA CGTGCAAGAG GGTCTAACCG ACGACGACTA CGACATGTAC 
TATGAGATCT GGCAGCAATT CGATCCGGAG GGCACCCAGT ACATACGCTA TGATCAGCTG 
TCCGAATTCC TGGACGTACT GGAGCCCCCG CTGCAGATCC ACAAACCGAA CAAGTACAAG 
ATCATATCGA TGGACATACC CATCTGTCGC GGTGACCTCA TGTACTGCGT CGACATCCTC 
GACGCCCTTA CGAAAGACTT CTTTGCGCGG AAGGGCAATC CGATAGAGGA GACGGGTGAG 
ATTGGTGAGA TAGCGGCCCG CCCGGATACG GAGGGCTACG AGCCCGTCTC ATCAACGCTG 
TGGCGTCAGC GTGAGGAGTA CTGCGCCCGG CTAATCCAGC ACGCCTGGCG AAAGCACAAG 
GCGCGCGGCG AGGGAGGTGG GTCCTTTGAG CCGGATACGG ATCATGGCGA TGGCGGTGAT 
CCGGATGCCG GGGACCCGGC GCCCGATGAA GCAACGGACG GCGATGCGCC CGCTGGTGGA 
GATGGTAGTG TTAACGGTAC TGCAGAAGGA GCTGCCGATG CCGATGAGAG TAATGTAAAT 
AGTCCGGGTG AGGATGCAGC GGCGGCGGCA GCAGCAGCAG CAGCAGCGGC GGCGGCGGGC 
ACGACGACGG CGGGAAGTCC CGGAGCGGGT AGCGCCGGGC GACAGACCGC CGTTCTCGTG 
GAGAGCGACG GGTTCGTGAC GAAGAACGGC CACAAGGTGG TCATCCACTC GCGATCGCCG 
AGCATCACGT CGCGCACGGC GGATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

para ( NM_001298404.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285333.1 Drosophila melanogaster paralytic (para), transcript variant BH, mRNA.

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CloneID	OFa21502
Clone ID Related Accession (Same CDS sequence)	NM_001298404.1
Accession Version	NM_001298404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paralytic, isoform BH
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGATC CGATATGATG ACGAGGACGA GGATGAAGGT 
CCACAACCGG ATCCTACACT TGAACAGGGT GTGCCAATAC CTGTTCGATT GCAGGGCAGC 
TTCCCGCCGG AATTGGCCTC CACTCCTCTC GAGGATATCG ATCCCTACTA CAGCAATGTA 
CTGACATTCG TAGTTGTAAG CAAAGGAAAA GATATTTTTC GCTTTTCTGC ATCAAAAGCA 
ATGTGGATGC TCGATCCATT CAATCCGATA CGTCGTGTGG CCATTTACAT TCTAGTGCAT 
CCATTATTTT CCCTATTCAT CATCACCACA ATTCTCGTCA ACTGCATCCT GATGATAATG 
CCGACAACGC CCACGGTTGA GTCCACTGAG GTGATATTCA CCGGAATCTA CACATTTGAA 
TCAGCTGTTA AAGTGATGGC ACGAGGTTTC ATTTTATGCC CGTTTACGTA TCTTAGAGAT 
GCATGGAATT GGCTGGACTT CGTAGTAATA GCTTTAGCTT ATGTGACCAT GGGTATAGAT 
TTAGGTAATC TAGCAGCCCT GCGAACGTTT AGGGTGCTGC GAGCGCTTAA AACCGTAGCC 
ATTGTGCCAG GCTTGAAGAC CATCGTCGGC GCCGTCATCG AATCGGTGAA GAATCTGCGC 
GATGTGATTA TCCTGACCAT GTTCTCCCTG TCGGTGTTCG CGTTGATGGG CCTACAGATC 
TATATGGGCG TGCTCACGCA GAAGTGCATC AAGAAGTTCC CGCTGGACGG TTCCTGGGGC 
AATCTGACCG ACGAGAACTG GGACTATCAC AATCGCAATA GCTCCAATTG GTATTCCGAG 
GACGAGGGCA TCTCATTTCC GTTATGCGGC AATATATCCG GTGCGGGGCA ATGCGACGAC 
GATTACGTGT GCCTGCAGGG GTTTGGTCCG AATCCGAATT ATGGCTACAC CAGCTTCGAT 
TCGTTCGGAT GGGCTTTCCT GTCCGCCTTC CGGCTGATGA CACAGGACTT CTGGGAGGAT 
CTGTACCAGC TGGTGTTGCG CGCCGCCGGA CCATGGCACA TGCTGTTCTT TATAGTCATC 
ATCTTCCTAG GTTCATTCTA TCTTGTGAAT TTGATTTTGG CCATTGTTGC CATGTCGTAT 
GACGAATTGC AAAAGAAGGC CGAAGAAGAA GAGGCTGCCG AAGAGGAGGC GATACGTGAA 
GCGGAAGAAG CTGCCGCCGC CAAAGCGGCC AAGCTGGAGG AGCGGGCCAA TGCGCAGGCT 
CAGGCAGCAG CGGATGCGGC TGCCGCCGAA GAGGCTGCAC TGCATCCGGA AATGGCCAAG 
AGTCCGACGT ATTCTTGCAT CAGCTATGAG CTATTTGTTG GCGGCGAGAA GGGCAACGAT 
GACAACAACA AAGAGAAGAT GTCCATTCGG AGCGTCGAGG TGGAGTCGGA GTCGGTGAGC 
GTTATACAAA GACAACCAGC ACCTACCACA GCACACCAAG CTACCAAAGT TCGTAAAGTG 
AGCACGACAT CCTTATCCTT ACCTGGTTCA CCGTTTAACA TACGCAGGGG ATCACGTAGT 
TCTCACAAGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGA GCATGGTGAC AACGAACTGG AGCTGGGCCA CGACGAGATC 
CTCGCCGACG GCCTCATCAA GAAGGGGATC AAGGAGCAGA CGCAACTGGA GGTGGCCATC 
GGGGATGGCA TGGAATTCAC GATACACGGC GACATGAAGA ACAACAAGCC GAAGAAATCC 
AAATATCTAA ATAACGCAAC GGACGACGAC ACTGCCAGCA TTAACTCATA TGGTAGCCAT 
AAGAATCGAC CATTCAAGGA CGAGAGCCAC AAGGGCAGCG CCGAGACGAT GGAGGGCGAG 
GAGAAGCGCG ACGCCAGCAA GGAGGATTTA GGTCTCGACG AGGAACTGGA CGAGGAGGGC 
GAATGCGAGG AGGGCCCGCT CGACGGTGAT ATCATTATTC ATGCACACGA CGAGGATATA 
CTCGATGAAT ATCCAGCTGA TTGCTGCCCC GATTCGTACT ATAAGAAATT TCCGATCTTA 
GCCGGTGACG ATGACTCGCC GTTCTGGCAA GGATGGGGCA ATTTACGACT GAAAACTTTT 
CAATTAATTG AAAATAAATA TTTTGAAACA GCTGTTATCA CTATGATTTT AATGAGTAGC 
TTAGCTTTGG CATTAGAAGA TGTACATCTG CCACAAAGAC CCATACTGCA GGATATTTTA 
TACTATATGG ACAGAATATT TACGGTTATA TTCTTCTTGG AAATGTTAAT CAAGTGGTTG 
GCGCTCGGCT TCAAAGTGTA CTTCACCAAC GCGTGGTGTT GGCTCGATTT CGTGATTGTC 
ATGGTCGTCG TTAATGCGCT GGTACAAGCT ATACCGTCCA TCTTCAATGT GCTATTGGTG 
TGTCTAATAT TTTGGCTAAT TTTTGCCATA ATGGGTGTAC AGCTTTTTGC TGGAAAATAT 
TTTAAGTGCG AGGACATGAA TGGCACGAAG CTCAGCCACG AGATCATACC AAATCGCAAT 
GCCTGCGAGA GCGAGAACTA CACGTGGGTG AATTCAGCAA TGAATTTCGA TCATGTAGGT 
AACGCGTATC TGTGCCTTTT CCAAGTGGCC ACCTTCAAAG GCTGGATACA AATCATGAAC 
GATGCTATCG ATTCACGAGA GGTGGACAAG CAACCAATTC GTGAAACGAA CATCTACATG 
TATTTATATT TCGTATTCTT CATCATATTT GGATCATTTT TCACACTCAA TCTGTTCATT 
GGTGTTATCA TTGATAATTT TAATGAGCAA AAGAAAAAAG CAGGTGGATC ATTAGAAATG 
TTCATGACAG AAGATCAGAA AAAGTACTAT AATGCTATGA AAAAGATGGG CTCTAAAAAA 
CCATTAAAAG CCATTCCAAG ACCAAGGTGG CGACCACAAG CAATAGTCTT TGAAATAGTA 
ACCGATAAGA AATTCGATAT AATCATTATG TTATTCATTG GTCTGAACAT GTTCACCATG 
ACCCTCGATC GTTACGATGC GTCGGACACG TATAACGCGG TCCTAGACTA TCTCAATGCG 
ATATTCGTAG TTATTTTCAG TTCCGAATGT CTATTAAAAA TATTCGCTTT ACGATATCAC 
TATTTTATTG AGCCATGGAA TTTATTTGAT GTAGTAGTTG TCATTTTATC CATCTTAGGT 
CTTGTACTTA GCGATATTAT CGAGAAGTAC TTCGTGTCGC CGACCCTGCT CCGAGTGGTG 
CGTGTGGCGA AAGTGGGCCG TGTCCTTCGA CTGGTGAAGG GAGCCAAGGG CATTCGGACA 
CTGCTCTTCG CGTTGGCCAT GTCGCTGCCG GCCCTGTTCA ACATCTGCCT GCTGCTGTTC 
CTGGTCATGT TCATCTTTGC CATTTTCGGC ATGTCGTTCT TCATGCACGT GAAGGAGAAG 
AGCGGCATCA ACGACGTCTA CAACTTCAAG ACCTTTGGCC AGAGCATGAT CCTGCTCTTT 
CAGATGTCGA CGTCAGCCGG TTGGGATGGT GTACTGGACG CCATTATCAA TGAGGAAGCA 
TGCGATCCAC CCGACAACGA CAAAGGCTAT CCGGGCAATT GTGGTTCAGC GACCGTTGGA 
ATAACGTTTC TCCTCTCATA CCTAGTTATA AGCTTTTTGA TAGTTATTAA TATGTACATT 
GCTGTCATTC TCGAGAACTA TAGTCAGGCC ACCGAGGACG TGCAAGAGGG TCTAACCGAC 
GACGACTACG ACATGTACTA TGAGATCTGG CAGCAATTCG ATCCGGAGGG CACCCAGTAC 
ATACGCTATG ATCAGCTGTC CGAATTCCTG GACGTACTGG AGCCCCCGCT GCAGATCCAC 
AAACCGAACA AGTACAAGAT CATATCGATG GACATACCCA TCTGTCGCGG TGACCTCATG 
TACTGCGTCG ACATCCTCGA CGCCCTTACG AAAGACTTCT TTGCGCGGAA GGGCAATCCG 
ATAGAGGAGA CGGGTGAGAT TGGTGAGATA GCGGCCCGCC CGGATACGGA GGGCTACGAG 
CCCGTCTCAT CAACGCTGTG GCGTCAGCGT GAGGAGTACT GCGCCCGGCT AATCCAGCAC 
GCCTGGCGAA AGCACAAGGC GCGCGGCGAG GGAGGTGGGT CCTTTGAGCC GGATACGGAT 
CATGGCGATG GCGGTGATCC GGATGCCGGG GACCCGGCGC CCGATGAAGC AACGGACGGC 
GATGCGCCCG CTGGTGGAGA TGGTAGTGTT AACGGTACTG CAGAAGGAGC TGCCGATGCC 
GATGAGAGTA ATGTAAATAG TCCGGGTGAG GATGCAGCGG CGGCGGCAGC AGCAGCAGCA 
GCAGCGGCGG CGGCGGGCAC GACGACGGCG GGAAGTCCCG GAGCGGGTAG CGCCGGGCGA 
CAGACCGCCG TTCTCGTGGA GAGCGACGGG TTCGTGACGA AGAACGGCCA CAAGGTGGTC 
ATCCACTCGC GATCGCCGAG CATCACGTCG CGCACGGCGG ATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285333.1
CDS	269..6436
Translation	MTEDSDSISEEERSLFRPFTRESLVQIEQRIAAEHEKQKELERKRAEGEIRYDDEDEDEGPQPDPTLEQGVPIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSEDEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKAAKLEERANAQAQAAADAAAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSIRSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFGIPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISYTSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEAGKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFKDKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNDTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQISDQPSEHGDNELELGHDEILADGLIKKGIKEQTQLEVAIGDGMEFTIHGDMKNNKPKKSKYLNNATDDDTASINSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGLDEELDEEGECEEGPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFARKGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDGDAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVLVESDGFVTKNGHKVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	NM_001298404.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paralytic (para), transcript variant BH, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298404.1

ORF Insert Sequence:

ATGACAGAAG ATTCCGACTC GATATCTGAG GAAGAACGCA GTTTGTTCCG TCCCTTTACC 
CGCGAATCAT TGGTGCAAAT CGAACAACGC ATTGCCGCTG AACATGAAAA GCAGAAGGAG 
CTGGAAAGAA AGAGAGCCGA GGGAGAGATC CGATATGATG ACGAGGACGA GGATGAAGGT 
CCACAACCGG ATCCTACACT TGAACAGGGT GTGCCAATAC CTGTTCGATT GCAGGGCAGC 
TTCCCGCCGG AATTGGCCTC CACTCCTCTC GAGGATATCG ATCCCTACTA CAGCAATGTA 
CTGACATTCG TAGTTGTAAG CAAAGGAAAA GATATTTTTC GCTTTTCTGC ATCAAAAGCA 
ATGTGGATGC TCGATCCATT CAATCCGATA CGTCGTGTGG CCATTTACAT TCTAGTGCAT 
CCATTATTTT CCCTATTCAT CATCACCACA ATTCTCGTCA ACTGCATCCT GATGATAATG 
CCGACAACGC CCACGGTTGA GTCCACTGAG GTGATATTCA CCGGAATCTA CACATTTGAA 
TCAGCTGTTA AAGTGATGGC ACGAGGTTTC ATTTTATGCC CGTTTACGTA TCTTAGAGAT 
GCATGGAATT GGCTGGACTT CGTAGTAATA GCTTTAGCTT ATGTGACCAT GGGTATAGAT 
TTAGGTAATC TAGCAGCCCT GCGAACGTTT AGGGTGCTGC GAGCGCTTAA AACCGTAGCC 
ATTGTGCCAG GCTTGAAGAC CATCGTCGGC GCCGTCATCG AATCGGTGAA GAATCTGCGC 
GATGTGATTA TCCTGACCAT GTTCTCCCTG TCGGTGTTCG CGTTGATGGG CCTACAGATC 
TATATGGGCG TGCTCACGCA GAAGTGCATC AAGAAGTTCC CGCTGGACGG TTCCTGGGGC 
AATCTGACCG ACGAGAACTG GGACTATCAC AATCGCAATA GCTCCAATTG GTATTCCGAG 
GACGAGGGCA TCTCATTTCC GTTATGCGGC AATATATCCG GTGCGGGGCA ATGCGACGAC 
GATTACGTGT GCCTGCAGGG GTTTGGTCCG AATCCGAATT ATGGCTACAC CAGCTTCGAT 
TCGTTCGGAT GGGCTTTCCT GTCCGCCTTC CGGCTGATGA CACAGGACTT CTGGGAGGAT 
CTGTACCAGC TGGTGTTGCG CGCCGCCGGA CCATGGCACA TGCTGTTCTT TATAGTCATC 
ATCTTCCTAG GTTCATTCTA TCTTGTGAAT TTGATTTTGG CCATTGTTGC CATGTCGTAT 
GACGAATTGC AAAAGAAGGC CGAAGAAGAA GAGGCTGCCG AAGAGGAGGC GATACGTGAA 
GCGGAAGAAG CTGCCGCCGC CAAAGCGGCC AAGCTGGAGG AGCGGGCCAA TGCGCAGGCT 
CAGGCAGCAG CGGATGCGGC TGCCGCCGAA GAGGCTGCAC TGCATCCGGA AATGGCCAAG 
AGTCCGACGT ATTCTTGCAT CAGCTATGAG CTATTTGTTG GCGGCGAGAA GGGCAACGAT 
GACAACAACA AAGAGAAGAT GTCCATTCGG AGCGTCGAGG TGGAGTCGGA GTCGGTGAGC 
GTTATACAAA GACAACCAGC ACCTACCACA GCACACCAAG CTACCAAAGT TCGTAAAGTG 
AGCACGACAT CCTTATCCTT ACCTGGTTCA CCGTTTAACA TACGCAGGGG ATCACGTAGT 
TCTCACAAGT ACACGATACG GAACGGACGT GGCCGCTTTG GTATACCCGG TAGCGATCGT 
AAGCCATTGG TATTGTCAAC ATATCAGGAT GCCCAGCAGC ACTTGCCCTA TGCCGACGAC 
TCGAATGCCG TCACCCCGAT GTCCGAAGAG AATGGGGCCA TCATAGTGCC CGTGTACTAT 
GGCAATCTAG GCTCCCGACA CTCATCGTAT ACCTCGCATC AGTCCCGAAT ATCGTATACC 
TCACATGGCG ATCTACTCGG CGGCATGGCC GTCATGGGCG TCAGCACAAT GACCAAGGAG 
AGCAAATTGC GCAACCGGAA CACACGCAAT CAATCAGTGG GCGCCACCAA TGGCGGCACC 
ACCTGTCTGG ACACCAATCA CAAGCTCGAT CATCGCGACT ACGAAATTGG CCTGGAGTGC 
ACGGACGAAG CTGGCAAGAT TAAACATCAT GACAATCCTT TTATCGAGCC CGTCCAGACA 
CAAACGGTGG TTGATATGAA AGATGTGATG GTCCTGAATG ACATCATCGA ACAGGCCGCT 
GGTCGGCACA GTCGGGCAAG CGATCGCGGT GTCTCCGTTT ACTATTTCCC AACAGAGGAC 
GATGACGAGG ATGGGCCGAC GTTCAAAGAC AAGGCACTCG AAGTGATCCT CAAAGGCATC 
GATGTGTTTT GTGTGTGGGA CTGTTGCTGG GTTTGGTTGA AATTTCAGGA GTGGGTATCG 
CTCATCGTCT TCGATCCCTT CGTCGAGCTC TTCATCACGC TGTGCATTGT GGTCAACACG 
ATGTTCATGG CAATGGATCA CCACGATATG AACAAGGAGA TGGAACGCGT GCTCAAGAGT 
GGCAACTATT TCTTCACCGC CACCTTTGCC ATCGAGGCCA CCATGAAGCT AATGGCCATG 
AGCCCCAAGT ACTATTTCCA GGAGGGCTGG AACATCTTCG ACTTCATTAT CGTGGCCCTA 
TCGCTATTGG AACTGGGACT CGAGGGTGTC CAGGGTCTGT CCGTATTGCG TTCCTTTCGA 
TTGCTGCGTG TATTCAAACT GGCCAAGTCT TGGCCCACAC TTAATTTACT CATTTCGATT 
ATGGGACGCA CCATGGGCGC TTTGGGTAAT CTGACATTTG TACTTTGCAT TATCATCTTC 
ATCTTTGCGG TGATGGGAAT GCAACTGTTC GGAAAGAATT ATCATGATCA CAAGGACCGC 
TTTCCGGATG GCGACCTGCC GCGCTGGAAC TTCACCGACT TTATGCACAG CTTCATGATC 
GTGTTCCGGG TGCTCTGCGG AGAATGGATC GAGTCCATGT GGGACTGCAT GTACGTGGGC 
GATGTCTCGT GCATTCCCTT CTTCTTGGCC ACCGTTGTCA TCGGCAATCT TGTGGTACTT 
AACCTTTTCT TAGCCTTGCT TTTGTCCAAT TTTGGCTCAT CTAGCTTATC AGCGCCGACT 
GCCGATAACG ATACGAATAA AATAGCCGAG GCCTTCAATC GAATTGGCCG ATTTAAAAGT 
TGGGTTAAGC GTAATATTGC TGATTGTTTC AAGTTAATAC GTAACAAATT GACAAATCAA 
ATAAGTGATC AACCATCAGA GCATGGTGAC AACGAACTGG AGCTGGGCCA CGACGAGATC 
CTCGCCGACG GCCTCATCAA GAAGGGGATC AAGGAGCAGA CGCAACTGGA GGTGGCCATC 
GGGGATGGCA TGGAATTCAC GATACACGGC GACATGAAGA ACAACAAGCC GAAGAAATCC 
AAATATCTAA ATAACGCAAC GGACGACGAC ACTGCCAGCA TTAACTCATA TGGTAGCCAT 
AAGAATCGAC CATTCAAGGA CGAGAGCCAC AAGGGCAGCG CCGAGACGAT GGAGGGCGAG 
GAGAAGCGCG ACGCCAGCAA GGAGGATTTA GGTCTCGACG AGGAACTGGA CGAGGAGGGC 
GAATGCGAGG AGGGCCCGCT CGACGGTGAT ATCATTATTC ATGCACACGA CGAGGATATA 
CTCGATGAAT ATCCAGCTGA TTGCTGCCCC GATTCGTACT ATAAGAAATT TCCGATCTTA 
GCCGGTGACG ATGACTCGCC GTTCTGGCAA GGATGGGGCA ATTTACGACT GAAAACTTTT 
CAATTAATTG AAAATAAATA TTTTGAAACA GCTGTTATCA CTATGATTTT AATGAGTAGC 
TTAGCTTTGG CATTAGAAGA TGTACATCTG CCACAAAGAC CCATACTGCA GGATATTTTA 
TACTATATGG ACAGAATATT TACGGTTATA TTCTTCTTGG AAATGTTAAT CAAGTGGTTG 
GCGCTCGGCT TCAAAGTGTA CTTCACCAAC GCGTGGTGTT GGCTCGATTT CGTGATTGTC 
ATGGTCGTCG TTAATGCGCT GGTACAAGCT ATACCGTCCA TCTTCAATGT GCTATTGGTG 
TGTCTAATAT TTTGGCTAAT TTTTGCCATA ATGGGTGTAC AGCTTTTTGC TGGAAAATAT 
TTTAAGTGCG AGGACATGAA TGGCACGAAG CTCAGCCACG AGATCATACC AAATCGCAAT 
GCCTGCGAGA GCGAGAACTA CACGTGGGTG AATTCAGCAA TGAATTTCGA TCATGTAGGT 
AACGCGTATC TGTGCCTTTT CCAAGTGGCC ACCTTCAAAG GCTGGATACA AATCATGAAC 
GATGCTATCG ATTCACGAGA GGTGGACAAG CAACCAATTC GTGAAACGAA CATCTACATG 
TATTTATATT TCGTATTCTT CATCATATTT GGATCATTTT TCACACTCAA TCTGTTCATT 
GGTGTTATCA TTGATAATTT TAATGAGCAA AAGAAAAAAG CAGGTGGATC ATTAGAAATG 
TTCATGACAG AAGATCAGAA AAAGTACTAT AATGCTATGA AAAAGATGGG CTCTAAAAAA 
CCATTAAAAG CCATTCCAAG ACCAAGGTGG CGACCACAAG CAATAGTCTT TGAAATAGTA 
ACCGATAAGA AATTCGATAT AATCATTATG TTATTCATTG GTCTGAACAT GTTCACCATG 
ACCCTCGATC GTTACGATGC GTCGGACACG TATAACGCGG TCCTAGACTA TCTCAATGCG 
ATATTCGTAG TTATTTTCAG TTCCGAATGT CTATTAAAAA TATTCGCTTT ACGATATCAC 
TATTTTATTG AGCCATGGAA TTTATTTGAT GTAGTAGTTG TCATTTTATC CATCTTAGGT 
CTTGTACTTA GCGATATTAT CGAGAAGTAC TTCGTGTCGC CGACCCTGCT CCGAGTGGTG 
CGTGTGGCGA AAGTGGGCCG TGTCCTTCGA CTGGTGAAGG GAGCCAAGGG CATTCGGACA 
CTGCTCTTCG CGTTGGCCAT GTCGCTGCCG GCCCTGTTCA ACATCTGCCT GCTGCTGTTC 
CTGGTCATGT TCATCTTTGC CATTTTCGGC ATGTCGTTCT TCATGCACGT GAAGGAGAAG 
AGCGGCATCA ACGACGTCTA CAACTTCAAG ACCTTTGGCC AGAGCATGAT CCTGCTCTTT 
CAGATGTCGA CGTCAGCCGG TTGGGATGGT GTACTGGACG CCATTATCAA TGAGGAAGCA 
TGCGATCCAC CCGACAACGA CAAAGGCTAT CCGGGCAATT GTGGTTCAGC GACCGTTGGA 
ATAACGTTTC TCCTCTCATA CCTAGTTATA AGCTTTTTGA TAGTTATTAA TATGTACATT 
GCTGTCATTC TCGAGAACTA TAGTCAGGCC ACCGAGGACG TGCAAGAGGG TCTAACCGAC 
GACGACTACG ACATGTACTA TGAGATCTGG CAGCAATTCG ATCCGGAGGG CACCCAGTAC 
ATACGCTATG ATCAGCTGTC CGAATTCCTG GACGTACTGG AGCCCCCGCT GCAGATCCAC 
AAACCGAACA AGTACAAGAT CATATCGATG GACATACCCA TCTGTCGCGG TGACCTCATG 
TACTGCGTCG ACATCCTCGA CGCCCTTACG AAAGACTTCT TTGCGCGGAA GGGCAATCCG 
ATAGAGGAGA CGGGTGAGAT TGGTGAGATA GCGGCCCGCC CGGATACGGA GGGCTACGAG 
CCCGTCTCAT CAACGCTGTG GCGTCAGCGT GAGGAGTACT GCGCCCGGCT AATCCAGCAC 
GCCTGGCGAA AGCACAAGGC GCGCGGCGAG GGAGGTGGGT CCTTTGAGCC GGATACGGAT 
CATGGCGATG GCGGTGATCC GGATGCCGGG GACCCGGCGC CCGATGAAGC AACGGACGGC 
GATGCGCCCG CTGGTGGAGA TGGTAGTGTT AACGGTACTG CAGAAGGAGC TGCCGATGCC 
GATGAGAGTA ATGTAAATAG TCCGGGTGAG GATGCAGCGG CGGCGGCAGC AGCAGCAGCA 
GCAGCGGCGG CGGCGGGCAC GACGACGGCG GGAAGTCCCG GAGCGGGTAG CGCCGGGCGA 
CAGACCGCCG TTCTCGTGGA GAGCGACGGG TTCGTGACGA AGAACGGCCA CAAGGTGGTC 
ATCCACTCGC GATCGCCGAG CATCACGTCG CGCACGGCGG ATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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para cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

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