Rbcn-3A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rbcn-3A
Entrez Gene ID	31557
Full Name	Rabconnectin-3A
Synonyms	CG3585,DmX,Dmel\CG3585,Dmx,FV10,X,anon-X
General protein information	Preferred Names Rabconnectin-3A Names CG3585 gene product from transcript CG3585-RB CG3585-PB Rabconnectin-3alpha Rbcn-3A-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5E1-5E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_132074.3	NP_572302.2	Rabconnectin-3A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DMXL2	XP_510406.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010490	XP_558026.3
Xenopus tropicalis (tropical clawed frog)	dmxl2	NP_001120527.2
Rattus norvegicus (Norway rat)	Dmxl2	XP_006243160.1
Homo sapiens (human)	DMXL2	NP_001167587.1
Bos taurus (cattle)	DMXL2	XP_002691013.2
Drosophila melanogaster (fruit fly)	Rbcn-3A	NP_572302.2
Caenorhabditis elegans (roundworm)	rbc-1	NP_001257246.1
Canis lupus familiaris (dog)	DMXL2	XP_005638465.1
Mus musculus (house mouse)	Dmxl2	NP_766359.2
Gallus gallus (chicken)	DMXL2	XP_003641895.1
Danio rerio (zebrafish)	LOC100536133	XP_003200528.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vacuolar ATPase (V-ATPase) and the V-ATPase regulator Rabconnectin-3 are required for subcellular tube formation, probably in a step resolving the intermediate compartment into a mature lumen.
Title: Intracellular lumen formation in Drosophila proceeds via a novel subcellular compartment.

Rabconnectin-3alpha and beta (Rbcn-3A, CG3585 and Rbcn-3B, CG17766) affects Notch signaling and trafficking through regulating V-ATPase function
Title: The vacuolar proton pump, V-ATPase, is required for notch signaling and endosomal trafficking in Drosophila.

The following Rbcn-3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rbcn-3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03116	NM_132074.3 Latest version!	Drosophila melanogaster Rabconnectin-3A (Rbcn-3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rbcn-3A ( NM_132074.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572302.2 Drosophila melanogaster Rabconnectin-3A (Rbcn-3A), mRNA.

Price & Availability↑

CloneID	OFa03116
Clone ID Related Accession (Same CDS sequence)	NM_132074.3
Accession Version	NM_132074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rabconnectin-3A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132074.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTGTC ATCAGATTTT GAGCGGCGCT TGCAATGCAG GCGACCGGTG CTTCGCCATT 
GGCTCCGTGG AAGGAATACC ATTTACAGCC TACGCCGCCG GCTGCAATGT GGTCATCCTG 
GCAAGCACTT TCGAGCGCGT CCAGATCATT CCGGGCGCCA ACCATGGCTA TGTCAAGATA 
TCCGCCCTGG ACTGCAGCAC CGATACCGGA AAAATAGCAG CAGCCTATGA GAATAAGATA 
TGCATTTTTG AGCCCACGCC GGTGATTGAG TCGAGCAAAC ACAATACCCA CTTTCTGGAT 
TACAGATGGG TGCAAACGGG CTCCTTCACC AGCCACTCGA ACGTGGTGAC CCTGTCCTGG 
AATCTGGAGG GCACCCGCCT CCTCACTGGC GGCACCAACA TCCAGCTGTG GAAGAGCAAG 
TCGCCCACTC ACCAGCAGGA GGAGGAGCAC ACGGGTGTCA AGTTCGAGAT TGGCGAGAGC 
AAGGAGCCCA AGCAGACGAG CAACGCCCAA TCGGAGGATG TGTCCACGTG GGAGAACATT 
TGGAGTTGCC AAACGGCCAT ACCCATTTAC CACATGGCAT TTAGTCCGGA TGGAACGCTC 
TTTGCCACCT GCGGACGCAA CGATCGGCTG GTAAAGATCT GGTATGAGAA CAAACAGGTT 
CTATTCCCGG CCAAGGGTTC CTCCAATGCC GGACCCACGC CATCATGTCG CCACGGAGTG 
GACGATGGTA CCACCTCAAG TTCTGGCGGT GAGCTAAACT TCACCTACGT GTACATCGCC 
CATCCGCGAG CCGTGTCCAA TATTGTGTGG CGCAAGACGA GCAAGTATAT GCCCAAGGGT 
TCGGTTGCCA ATATGCTGGT CACGTCCTGT CTGGACAACA TATGTCGCAT CTGGATCGAG 
ACGGTGCTGC CGGATGACGG AATGGTGAAC ATGACCCAGT TCGATCCGAT GGCATCGCAG 
AACCCCAAGT TCCGTACGCA TCGGCACAAG CATCGGTTCA TGCAGAGGCT GAAGCACATG 
AAGACCTGCT TCCATATCCG TCGGCACATG AAGGCCGGAG CGAGTGCCGT GCCGGGTGCA 
GCCAGTGCAG GCGCTGCCAG TGGCGGTGGA ACCGCCGGAG CAATGGGCGG CATGGGTAGC 
TCCCCGGCTA GCTATACAAA CTACCTGGGA CCAATACCCT CGCTACCATC CTCGTGCAGC 
GTCCACGATT TTCACTCTTA CGGATTCCAG GGATCGGGCG TTACACCCGG TATGCACTTC 
CACCTGGCCG CGTCGATAAA CGCGGAAACG GACATCCCGC TGGTGCCATC GATGCAGACC 
AGCGATCCGG CCAATCAGAA TGTCTTTATC CTGCACTGGC TGAACAACAA GGAGATGCAT 
TTCACGCTGC AGGCGGAGGC TATACTCCAG GAGCTCACCA AGAAGGTGAT CGACGAGGAA 
AACGGTCATG GGAATGCCTC CAGCGGCGGC GTCGATCTAC CGGACGATGC CCACGGTTCC 
CATTCGCAGG CACATGCGCA CAAGAAGTAC CTGCGCTCAT CCAAATCGGT GTCCGTGGAC 
GATAGCGGCG AGGAGTTTAG CCGGTATTCG CAGCACACGG CGGGCACGGG AATGGGTGGC 
GGAGGTGGCG GCGCCGGGCT GCATCTGAAC TCCATGTCCA ATACGACGTC ACAGAACTCG 
CTCAACAATG AGCCGGCCCA TCCGATTACG CAGCTAGTGG ATTCGTTGGA CAATAAGATC 
GAGTGTTTGT TGCGCGACTG GCACCAGAGC CCGGATCTGC TCTTCGCCAT TCATCCGGTG 
GACGGGAGCT ACCTGATCTG GGTGATCGAG TGGCTGGATG ACTACTATCC GGGCTCGTTC 
AGGCAGGCGC AGGTCTCCTT CTCCACTCGG ATTCCTTCGG CCTTCCCTCT GGGCGACGCC 
ATGTCCATGT CCACCACGGT CAGCCTCTTC AACACGGGCA CGCATCCGCT GGCCTTCCGC 
GACATAGCGA ACAATGTGCG CAGCAAGGAT GAGTTTCACA AGGGACACGC GGATGATTCC 
TCCCTGAAGA GTGACCAGCA TTCGGGGCAG ACCTTCGAGA GGCACGACGA AGAGCAGGAG 
GATGCTGCCG ATGACGATGG AGATGGAGAT CGCGAAGGCG ACGCAGATCA AGATGAGGGT 
GGTGGCGATC GCAGTGCTGC GGCTAGTGGA GGCGGAGCAG CCGAGCACAG CAGTAGCTCG 
CAAGATTCGG CTGCAACTGG AGCCAGTGCA TTGCCCCTGA ACGTTTCTCC CTCGGTTTCC 
ATGGTGACCA AGCACTCAAA TGGAACCCTT AACCTTTGGC AGCTGACGTT TGCCTTCAAG 
ACGAAGTTTA CCCAAGTATT AAGCATTGGC CACGTAAGTC GTGCCTCCGG CCATCGGTTC 
CGGGTGAACG ACATCACCTG CCACCCGGTG TTACCGCTGC TCGTCTCCAC CTCGCACCAC 
AACCTGCCCG ATTCGGAGGC CAAGTCACCA ATGTCACTGT TTGAGCAACC CCTGAGTGGT 
GGACTTCCCT CGGGCGGCGG GACTGGAGCC GGTCCCTCTG TTGGTTCCAA TGGAGACAAA 
GATGTATTTA CGCCCACGGG CTTTTGTTCG GAGTTAATCC TCTGGCGTGT GGACTCCGTG 
GGACCGCTGT GCCATTCGGG CGGTGTCAGC GAGCTGGCGA GGATTAACTC GCCGGAGATC 
TCGGCCTTCA GCAATGTAGC CTGGATTCCC ACCCTCCTGC CGAGCACCAC ACTGGGCAGC 
TACAGTAACT CCCCGTCCGC CTGCTTCGTG GCCAGCGATG GCGAAAATCT GCGCGTCTAT 
CAGGCAGTGA TCGATGCCCG CACCCTGCTG GCGGAGATCT CCTGCTCGGA GAACCTAAAC 
ACGCTGCACA AATCGCATTC GCTGTCGTCG ATGATCTCGA ATGCTTCGTC TACGATGCGG 
GCCCAGCGAT CGGCGCTGCA CGACAAGCTC AAGGTTGTGT CGCAACAGTC GACGGCGAGA 
CCCGGTTGCA TCTTGCAGCT GGACGCCATC TCGGATGCCA AGCACGACTG GCAAAATACG 
CAGTTCCTGC ACGTGTTCCA GGCGCAGTTG ATAACGGGTG GCCAGAGGAC GCCCCAGGCG 
GATCCCCATG ATTTGGAGGA GCCACGTCTG AACGCTCTGC TCGAGTCGGA CATGGATGCC 
ATCGTCGATT TGCAGCGGAA TGCGGACTTC GAGGAGCCCT TCTACATTGT GAACATCGAG 
AAAACCCTGC GCGGCAGCAC CATACACATG TGGCGCATAG TCATCAGCTC GAAGCAGCAG 
AGCTCGTTCC TTTCCGAGAC GGCCATGTAT GTGCCGGATA GTAATCTTAC CCAGGAGCCA 
GATGAGGATG GCGCCGGTGG TAATCCTAAT CCCAGGCGAA TGTCGCAGGG AGCGGAAACA 
CTGACGGGAG CCGAGCACTT TGGTCCCCAA GTGGAGGCGC CTCACATTTC GATTACCACC 
AAGAAGGTGT GCACTCAAGA GCTGCCACTG CCCGAGGGCG TGGACGTCAT CCATGCCTCT 
CCGGCCGCCG GTCATCTGAG CAGCTCCAGC ATTTACCCAG CCTGCTTTGC GCCGTACATC 
ATTGTGACCG CCTGCTCGGA TTCCATTTCG CGTTTCTGGA AGTGCGAGCC CATCGGTGAT 
GAGGATGATC CGAATCCGGA AGGCGATGCC TATCACTGGA GCGAGTGGAA GATGTTTAGT 
CGCATTCAGC ATTCGGCCAT CGAGATTCCG GGCCAGCCGC TGAACATCAG TGCCGCCTAT 
TCCGGCCGGA TAGCCTGTGC CTACAAGTAC GGCAAGAGTT TTACCCGACC CAATAAGGGA 
CCCGATCCGG ATTCGCGTTA CGTTAACCTC TGCGTGGCCA TCTACGAGTG CGAGAGCTCC 
GGCGGCAGTG AGTGGGTGCT GGAAGACACC ATTCACCTGA AGAATGTCCA TTTGCCGCGA 
ATCAATATTG GTCATGGCAT CGATCTGAGT TACCTGCACG ACAGTCGCCT GCTGGCGAAG 
AAGCAACGGC TGAACCAGGT CCTGCACACC TTCGCACACG ATCCGGAAAG CAGATCGCCG 
CGGAGTGGCG AGACCACACC CGAGTTGGCC GTCAATAGGC AACCGTCGGC CGCTGCATCC 
GGATTGCTAA CCGTGCCCAG CTTCAGTACG CTGCAATCTC TTCGCAAGAG CATAGCCGAA 
AATGGGAACA CCTGTCCGCT GACCCAGAAG CATTTGGTCC AACTGGACTG GGTGAGCAAG 
GAGGATGGTT CACACATCCT TACCGTTGCG GTGGGCAGCA AGATTCTGCT GTACACCCCA 
GTGAGCTCGG ACATTGCCCA GGCGAACATC AAGGCGATGA AGGAGTCGCG TTCAGTGAAT 
CGTCCCATCC TGCGAAAGGC TAGTTCGCTG GCGCAGCCCA ATTTTGTAGA CGAGATCCGT 
TGGATGAAGT TGCGGCAGAT AGACCTCCAG ACCGCCGATG GTCTGCCGCC GCTGCCCATG 
CAGATCTCTT GGGTGCGCGA TGGCATTCTA GTGGTGGCCA TGGACTCCGA GATGCACGTT 
TACTCACAGT GGAAGCCCCA TTATTCCTCG CACTTTGCCG CTGCAGCGGC TGCTGCTGGC 
GAAGATGTAC CGGATACTCG TAACCTGAGG GACGAAGATC TGCGTTCGCT GGCCAACGAG 
TCCACGCAGC TGCGCCTCAA GAACGTGGCG TCCATGCCGC TAATCAGCAA GGTGAGCACG 
GCCAATCTGC AGCTGCTGTC GCACGACAAG AAGCGTCGTC TGGGCAACAC CAGCATGGCC 
AATATGAACG GAGCCGGCGG TGCAGGAGGA ACTGGAACGT ATGGCGGTGG CGCTGATGAC 
GGCGGCAACG ATGACTACAT GACGGACTTC GGTCTGTTCC AGGCCTCTCG CATCGCATGT 
CCAGTGTTGC CGCAATACCA TCCCAAGCAG CTGATGGAGC TGCTTAACTG TGGCAAGATC 
CGTTGGGTGA AGGCCATCCT GGCGCATCTG GTGCGCTGCA TGAGTGGCAA TAGTTCGAGT 
ACAGTGAACC AGGACGATGA TGGCTATGCC AGACAGCGCA GCTGGTCGAG ATCTCGTACC 
CTGAGCATTA GCTACACAGC GGATCAGAAC ATCCAGGCGG AGCATCGCGG CAGCACCACC 
CAAATTCCCG AGGAGCTGAT GCTGGACTAC GCAGAGATCA ATTCGATTCC GCCATTACCC 
TTGTGGGTGC TCCTCAATGC GGATCGTGAG ACGCCGGGTC AGAGCAACAA CTATGCTGAG 
GGCGACAAGG ACTACGACGA GCTCTTTGAC ATGCACAACT CGGAGGACAA CCTGGATGAG 
CTGCTCAGCG AGGGTGATGA GAAGAAGCGC CAGGAGCGCC GTCTCTCTCT GCCCGAGAAG 
CACTCCATTT CGCACTTTGG TCCGCGGCAG GGACAGCTTC TTTCGCGCCT GCTGACCCAC 
ACCCATTTGC CGGGACTCTC CTCGCTGGAC CAGATGCATC TGCTGGCTCT GGCCGATACG 
GTGGCCACGT GCAATACGGA CTTTTCCGAC AAGTTTGCCG CCGATCAGGG CAAGTCGGGC 
GGAATGGCCG GCGGAGCTGG ATCGGGAGCC GTTAAGGACG GTAGCACCAG TACTGATTCC 
CTAGATGATT GCGGTCTACG CTTTCTGCTG GCCATGAAGC ACTTCACCTA CCTGCTGCGA 
TGCCTGCCGC TCCAGCAGAG GGCGCAGTTC CAGCGCCAGG GCGTGGGCAC CTCCAACATC 
GTGTGGGCCT TCCACTCGGA GAGCGAGGAG GAGCTGCTTA ACCTCATTCC TAGCTACACC 
AAAGGCGATC TCCGCTGGGC AACGCTGCGG GATCTGGGCG TCGGCTACTG GCTGAAGAAC 
ATTAACACGC TGAGGAGATG CGTCGAGAAG CTGGCCAAGT GCGCCTACCA GCAGAAGCAG 
GAGCCACTGG ACGCGGCCAT CTATTACCTG GCCATGAAGA AGAAGTCCCT GGTCTGGGGT 
CTGTTCCGTT CACGTCGGGA CGAGAAGATG ACCGCCTTCT TTGGCAACAA TTTTGCCGAG 
GATCGCTGGC GCAAGGCGGC ATTGAAGAAT GCCTTTGTGC TATTGGGCAA GCAGAGATTC 
GAGCACGCGG TGGCCTTCTT CCTGCTGGCC AATAGCCTCA ACGACGCCAT TGAAGTGTGC 
ATGAACAAGC TGGAGGACTT CCAACTGGCC CTGATCATAG CTCGACTCTA TGAGGGCGAC 
GGCGAGGGCT GCTACTACCA CCACTTGCTG CACGAGCACA TTTTGGGCAC GGATGCGGCA 
ACTGGGCGCT GTGATCTCAG TCGCGCGCAT CCCGATCCCT TCTTGCGCTC GATGACCTAC 
TGGACGATCA AGAAGTATCA GGAAAGCTTG AACACCCTGC TCCTGAACAA CGTGGGCAGC 
CTGCACAGCA GCTACAGGGA GGAGGATCTA CTGCGTCCCG AACAACAGGC CACAAATCCC 
AATGTCTTTA ACTTTTACAT CTATTTGCGC ACCCATCCGC TGCTCATCCG GCAGAACATC 
GCTTTGTCGG CGCAGGAGAA GCGCATTGCG CATGTGGTCT TGTCCGGTTT CAATTATGCC 
GGCGATGCTG CTCCCAGTGC CGTGGCCTGT GACAAACAGT TGCAACTGGA GGATTCAATA 
ACGCCCATTG AACGGCAATT GTACTTCACC ACCGCCCATG GGCACTTCAA GAGCGGGTGC 
CCGGCGCTGG CGCTCGAGGT GCTCAACAAG CTGCCCCAGA AGATCAGCGA CGATTCTGGT 
GGCAGTGTGG CCGGCAGCCA GGCGCAGGAG CGTGCCCAGC AGAACAAGGA GGAGCTGATC 
AACACGGGCA TTATGGACCA GTGGGGAGCA ACTCCTGCCG CTGCGTCAAA CACTGCGGAT 
GCCTTCGACT GGGGCGCACC CGTGTCCAGC GAGCCGGAAG CCAAGTTTGA AATAAAATGG 
GATGATGAGG ACGAGGAAGA AGACCCAGAT TTGGAGCCAG ACGAACCGGA GCTAGCCACG 
CCACAGCCGC AGGCCCCAAT TCTTGGCAGA GGCAAATCGA ATGGCAACGA CACAAAGATG 
GACATCATGG CGCAGCAGCT TAAGTTCGTC GCCTGTCTGA AGATTCTTAT GGAGGAGTTG 
TCTACGCTGG CCACCGGTTT TGAGGTGGAT GGCGGCCAGT TGCGCTATCA GCTGTACATG 
TGGCTGGAGC GGGAGGTGGA GGCACTTAAG CAGCTGTGCA ACTACTGCAG TCAAGCGGAG 
CAGTCGAGTC ACGAATCTGG TGATGCTGAT GCGGAGGCCA GGGACAAGGA GATGAACGCC 
AGCATCTATC CCAGCACCGA GCGACCTACG CTTCACGAGA TCCTCATGCA GGACAAGCAA 
GACTTTGAGG CCAAGGTGAT GCGGGCGGCC AAACGCAAGC GATGGCTCAA GGCAAATGAA 
ACCCTACTCC GCACACTGCT GAGTTATTGT TCCCTACACG GCGCCAGTGG TGGTGGCCTG 
GCATCCGTGC GCATGGAGCT GGTACTGCTC CTGCAGGAGC TCCAACAGGA GAAGACCCAA 
CAGCAGCTTC TGAGTCCGCT ACCCTTCCCA ACCACTTTGC CTCTGCTAAG CGCCTGTGTG 
GCGGGCAACA AGACGGTGAT CGCCGATCCC ATCAAGTACC TGCAGTCCCA GACGGTGGAC 
ATGCTGCAGA GCATCATTAA GGTGCTGCCC TTCCCAGGAA TCGAGCCCAG TGCTTTGAGC 
GAGATCTTTG TTTTGCGGGA TCTTGCCGTG GCGCTGTCAT CCTGCATTTA CCAGTCGCTC 
TGCGATTCCG AGAGCTTTGT GGTAAACAAC TCGGCCTTCA ACGGTTACCC CAGTCCGGGA 
ATGGAGAACA TAGCTAAGAT AAACTCATCG TTCGAGTGCA GCTATTTGGT GGGCAGCCGG 
AATGCCTATG GCCGCAGGCG CAAGTACTCT ACGGATGAGC CAGCAGGCGT GTGCACCACG 
CCTTCCAAGT GGCCGGGTGT AACCAATCTC CGTGCGCTTT TGGCGCGGGA AAAGGATGAG 
GATGTGCCTA AGCTAAATGT GCTGCTGCTG GAGTCCTTTG TTTCCACCTA TATGGCGCTC 
TTCATTTACT CGCTATCCAC CTGCGATAGT CGTTTGTTGT ACCGCCTGAG TGGCCAGAGT 
TTCAACAACG AGACCTGGTC CACTCTTTTC GGCGGCGGCA TGAAGAAGTT GCTTATAAAG 
CCCGCTGCTG CACCGCCTCC CACCCAAATG GTGACCGGAG GAGCAGGAGC TGGCGGAGCA 
AGTGGTGGTA CTGGTTCCAC ATCCGAAGAG CCGACCGATG AGAATAGCGT TTGGAACACG 
GTTACTTCCA TTACAAAGCA ACGCATGAAA CTGAACATGA AAATTCTAGG CAGCTTTAGC 
CAGAACAGCA CTTCAAGCAA CATGAAAGAG GACAAGCCGA CATACCGGGA GCAGTTTATG 
CCACCGGAGA CGTCCATGCT ATCTTACTTC CTTACCAAAC CGCCCACAAC CGAATGCGAT 
GACTACGATA CGGACGACTC GCACGAGTCG GACAACGAGG AGGAGGAGGA GGACGACGAT 
GATGTGAATG TGAGTCGATC GCAGCTGAAG GCCAAGGATA ACACGGAGCA CACTAATCCC 
ACGTCGTACT CGTGGAGTAT CCTGCGCCTG GCGCTCATCA AGATCAGCAC GCACAAGATC 
CAGGAGCTGG TCAAGGTGGC CGGCATCGAA CTGCAGGATT TGCCTGTGAT CAGTCCACTC 
TCCCACGAGG TGCTGAGGAC CCTCAATCGC TGGCAGGACT TTGCCCTGAG TGACCTCATA 
GCTCGTGGGC CACCGTCCCG TAATTATATA CCAGGCTGCT ATGCGGAGAG CGGCATCAAC 
GGATTGGCCA TCCACAAGTA CCGCTCGCTC CTGAATAAGG ACAACACACC ATTCGTTTCC 
GGTTCATCGG CTGGACCCAT AAGGCGACTG TGGAACTATT TGGTGCGCCA GGAGCCTGCC 
CAGGAGGTCT TCATCAAGAG CATCTTTGTC AAGAACATGG AGCCCAGCAC GCGAGGATCG 
CACCACACCA ACAACGACAA CGAGACGGAT GAGGGTCAAT CTCATTCGAC CAACGAGAAT 
ACCGACCACA TCCGCATCAT TCACAAGGAC CACGAGTCCA TATTGTCCTT CTGTTTGAAG 
AACAACAGCT CCTCAATGAT TGCCTTTGCG AATCCGCGTG AGATCCAGGA GTTCGACATC 
TCACTGCTGC TGGAGTCGCC GAATTGGTAC GAGGATGAGT GCGACTACGA CATGATGAAC 
TTGTCCAAGG ACGCGGACAC CAACAGCTCG TCCTTCCTGA TCATTCAAAC GCAAGAGCAA 
AACCAATTTG GAAGCAATAG CAATGCCTAC AGCGAGTCCA ATGCTAGCAC ACAAAGTGCT 
TCGCAATCGG GACGCGGCAC ATCCATGGTG CTGCGCCACA AGGTGGACAA TGTGAAGCGC 
ATGAGCGCCC ATCCGCTGAT GCCGCTGTAT CTTACTGGAG GTCAGGACGG ATCCGTGCAG 
ATCTGGGAGT GGGGTCACCA GCAGCCTGTC TGCTCGCCCC GGACTTCGGG AACCTTTGCC 
AAGGTGACGC GCTGCCGCTT CTCCGAGCAG GGCAATAAGT TCGGCATCGG CGATGGCGAC 
GGAAAGCTCA GTCTCTGGCA GGCGGGCATC GCCTCGCAGA ATAATCGTTC GTTTATAAGC 
TACCAGTGCC ACAACAAGGC GTTGTCCGAT TTCGTGTTCC TCGGCTCGTG CAGTCTCCTG 
GCTAGCGCTG GTCAAAGTTC CGAGAACAAG AACATCAACA TCTGGGACAC ATTGCTTCCC 
CACAAAAAGT CCTGTGTATC GGCCTTCACC TGCCACGACC AGGGCTCGTC CTGTCTGGTG 
TTTGCTCCGC AGCACCAGGT GCTCATCTCG TGCGGCAAAC GCGGCGATGT TTGCGTCTTC 
GATGTGAGAC AACGTACGCT GCGTCATCGC TATCAGGCCC ATGATAGCAC CATCAAGTGC 
ATTGCTCTGG ATCCGCACGA GGAGTTCTTT GTCACTGGCT CCATTGAGGG TGACATTAAG 
ATCTGGGACA TGAATCAGTT CATGCTGATC AATACGTTCC CGCATGAGCA TGCCAAGAAC 
GGATTCTTTA AGCACACTGG CCAGGGCGTC AGCCAGGTGT ACGTGGATGC CTTCGGACGG 
CTCTTCTCGT GCGGCTCCGA TGGCTGCATG AAGGTCCGTC TGCTGGTGGA GAAAGACAAC 
ATTGTTCACT CCGTGTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572302.2
CDS	140..10420
Translation	MNCHQILSGACNAGDRCFAIGSVEGIPFTAYAAGCNVVILASTFERVQIIPGANHGYVKISALDCSTDTGKIAAAYENKICIFEPTPVIESSKHNTHFLDYRWVQTGSFTSHSNVVTLSWNLEGTRLLTGGTNIQLWKSKSPTHQQEEEHTGVKFEIGESKEPKQTSNAQSEDVSTWENIWSCQTAIPIYHMAFSPDGTLFATCGRNDRLVKIWYENKQVLFPAKGSSNAGPTPSCRHGVDDGTTSSSGGELNFTYVYIAHPRAVSNIVWRKTSKYMPKGSVANMLVTSCLDNICRIWIETVLPDDGMVNMTQFDPMASQNPKFRTHRHKHRFMQRLKHMKTCFHIRRHMKAGASAVPGAASAGAASGGGTAGAMGGMGSSPASYTNYLGPIPSLPSSCSVHDFHSYGFQGSGVTPGMHFHLAASINAETDIPLVPSMQTSDPANQNVFILHWLNNKEMHFTLQAEAILQELTKKVIDEENGHGNASSGGVDLPDDAHGSHSQAHAHKKYLRSSKSVSVDDSGEEFSRYSQHTAGTGMGGGGGGAGLHLNSMSNTTSQNSLNNEPAHPITQLVDSLDNKIECLLRDWHQSPDLLFAIHPVDGSYLIWVIEWLDDYYPGSFRQAQVSFSTRIPSAFPLGDAMSMSTTVSLFNTGTHPLAFRDIANNVRSKDEFHKGHADDSSLKSDQHSGQTFERHDEEQEDAADDDGDGDREGDADQDEGGGDRSAAASGGGAAEHSSSSQDSAATGASALPLNVSPSVSMVTKHSNGTLNLWQLTFAFKTKFTQVLSIGHVSRASGHRFRVNDITCHPVLPLLVSTSHHNLPDSEAKSPMSLFEQPLSGGLPSGGGTGAGPSVGSNGDKDVFTPTGFCSELILWRVDSVGPLCHSGGVSELARINSPEISAFSNVAWIPTLLPSTTLGSYSNSPSACFVASDGENLRVYQAVIDARTLLAEISCSENLNTLHKSHSLSSMISNASSTMRAQRSALHDKLKVVSQQSTARPGCILQLDAISDAKHDWQNTQFLHVFQAQLITGGQRTPQADPHDLEEPRLNALLESDMDAIVDLQRNADFEEPFYIVNIEKTLRGSTIHMWRIVISSKQQSSFLSETAMYVPDSNLTQEPDEDGAGGNPNPRRMSQGAETLTGAEHFGPQVEAPHISITTKKVCTQELPLPEGVDVIHASPAAGHLSSSSIYPACFAPYIIVTACSDSISRFWKCEPIGDEDDPNPEGDAYHWSEWKMFSRIQHSAIEIPGQPLNISAAYSGRIACAYKYGKSFTRPNKGPDPDSRYVNLCVAIYECESSGGSEWVLEDTIHLKNVHLPRINIGHGIDLSYLHDSRLLAKKQRLNQVLHTFAHDPESRSPRSGETTPELAVNRQPSAAASGLLTVPSFSTLQSLRKSIAENGNTCPLTQKHLVQLDWVSKEDGSHILTVAVGSKILLYTPVSSDIAQANIKAMKESRSVNRPILRKASSLAQPNFVDEIRWMKLRQIDLQTADGLPPLPMQISWVRDGILVVAMDSEMHVYSQWKPHYSSHFAAAAAAAGEDVPDTRNLRDEDLRSLANESTQLRLKNVASMPLISKVSTANLQLLSHDKKRRLGNTSMANMNGAGGAGGTGTYGGGADDGGNDDYMTDFGLFQASRIACPVLPQYHPKQLMELLNCGKIRWVKAILAHLVRCMSGNSSSTVNQDDDGYARQRSWSRSRTLSISYTADQNIQAEHRGSTTQIPEELMLDYAEINSIPPLPLWVLLNADRETPGQSNNYAEGDKDYDELFDMHNSEDNLDELLSEGDEKKRQERRLSLPEKHSISHFGPRQGQLLSRLLTHTHLPGLSSLDQMHLLALADTVATCNTDFSDKFAADQGKSGGMAGGAGSGAVKDGSTSTDSLDDCGLRFLLAMKHFTYLLRCLPLQQRAQFQRQGVGTSNIVWAFHSESEEELLNLIPSYTKGDLRWATLRDLGVGYWLKNINTLRRCVEKLAKCAYQQKQEPLDAAIYYLAMKKKSLVWGLFRSRRDEKMTAFFGNNFAEDRWRKAALKNAFVLLGKQRFEHAVAFFLLANSLNDAIEVCMNKLEDFQLALIIARLYEGDGEGCYYHHLLHEHILGTDAATGRCDLSRAHPDPFLRSMTYWTIKKYQESLNTLLLNNVGSLHSSYREEDLLRPEQQATNPNVFNFYIYLRTHPLLIRQNIALSAQEKRIAHVVLSGFNYAGDAAPSAVACDKQLQLEDSITPIERQLYFTTAHGHFKSGCPALALEVLNKLPQKISDDSGGSVAGSQAQERAQQNKEELINTGIMDQWGATPAAASNTADAFDWGAPVSSEPEAKFEIKWDDEDEEEDPDLEPDEPELATPQPQAPILGRGKSNGNDTKMDIMAQQLKFVACLKILMEELSTLATGFEVDGGQLRYQLYMWLEREVEALKQLCNYCSQAEQSSHESGDADAEARDKEMNASIYPSTERPTLHEILMQDKQDFEAKVMRAAKRKRWLKANETLLRTLLSYCSLHGASGGGLASVRMELVLLLQELQQEKTQQQLLSPLPFPTTLPLLSACVAGNKTVIADPIKYLQSQTVDMLQSIIKVLPFPGIEPSALSEIFVLRDLAVALSSCIYQSLCDSESFVVNNSAFNGYPSPGMENIAKINSSFECSYLVGSRNAYGRRRKYSTDEPAGVCTTPSKWPGVTNLRALLAREKDEDVPKLNVLLLESFVSTYMALFIYSLSTCDSRLLYRLSGQSFNNETWSTLFGGGMKKLLIKPAAAPPPTQMVTGGAGAGGASGGTGSTSEEPTDENSVWNTVTSITKQRMKLNMKILGSFSQNSTSSNMKEDKPTYREQFMPPETSMLSYFLTKPPTTECDDYDTDDSHESDNEEEEEDDDDVNVSRSQLKAKDNTEHTNPTSYSWSILRLALIKISTHKIQELVKVAGIELQDLPVISPLSHEVLRTLNRWQDFALSDLIARGPPSRNYIPGCYAESGINGLAIHKYRSLLNKDNTPFVSGSSAGPIRRLWNYLVRQEPAQEVFIKSIFVKNMEPSTRGSHHTNNDNETDEGQSHSTNENTDHIRIIHKDHESILSFCLKNNSSSMIAFANPREIQEFDISLLLESPNWYEDECDYDMMNLSKDADTNSSSFLIIQTQEQNQFGSNSNAYSESNASTQSASQSGRGTSMVLRHKVDNVKRMSAHPLMPLYLTGGQDGSVQIWEWGHQQPVCSPRTSGTFAKVTRCRFSEQGNKFGIGDGDGKLSLWQAGIASQNNRSFISYQCHNKALSDFVFLGSCSLLASAGQSSENKNINIWDTLLPHKKSCVSAFTCHDQGSSCLVFAPQHQVLISCGKRGDVCVFDVRQRTLRHRYQAHDSTIKCIALDPHEEFFVTGSIEGDIKIWDMNQFMLINTFPHEHAKNGFFKHTGQGVSQVYVDAFGRLFSCGSDGCMKVRLLVEKDNIVHSVY

Target ORF information:

RefSeq Version	NM_132074.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rabconnectin-3A (Rbcn-3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132074.3

ORF Insert Sequence:

ATGAATTGTC ATCAGATTTT GAGCGGCGCT TGCAATGCAG GCGACCGGTG CTTCGCCATT 
GGCTCCGTGG AAGGAATACC ATTTACAGCC TACGCCGCCG GCTGCAATGT GGTCATCCTG 
GCAAGCACTT TCGAGCGCGT CCAGATCATT CCGGGCGCCA ACCATGGCTA TGTCAAGATA 
TCCGCCCTGG ACTGCAGCAC CGATACCGGA AAAATAGCAG CAGCCTATGA GAATAAGATA 
TGCATTTTTG AGCCCACGCC GGTGATTGAG TCGAGCAAAC ACAATACCCA CTTTCTGGAT 
TACAGATGGG TGCAAACGGG CTCCTTCACC AGCCACTCGA ACGTGGTGAC CCTGTCCTGG 
AATCTGGAGG GCACCCGCCT CCTCACTGGC GGCACCAACA TCCAGCTGTG GAAGAGCAAG 
TCGCCCACTC ACCAGCAGGA GGAGGAGCAC ACGGGTGTCA AGTTCGAGAT TGGCGAGAGC 
AAGGAGCCCA AGCAGACGAG CAACGCCCAA TCGGAGGATG TGTCCACGTG GGAGAACATT 
TGGAGTTGCC AAACGGCCAT ACCCATTTAC CACATGGCAT TTAGTCCGGA TGGAACGCTC 
TTTGCCACCT GCGGACGCAA CGATCGGCTG GTAAAGATCT GGTATGAGAA CAAACAGGTT 
CTATTCCCGG CCAAGGGTTC CTCCAATGCC GGACCCACGC CATCATGTCG CCACGGAGTG 
GACGATGGTA CCACCTCAAG TTCTGGCGGT GAGCTAAACT TCACCTACGT GTACATCGCC 
CATCCGCGAG CCGTGTCCAA TATTGTGTGG CGCAAGACGA GCAAGTATAT GCCCAAGGGT 
TCGGTTGCCA ATATGCTGGT CACGTCCTGT CTGGACAACA TATGTCGCAT CTGGATCGAG 
ACGGTGCTGC CGGATGACGG AATGGTGAAC ATGACCCAGT TCGATCCGAT GGCATCGCAG 
AACCCCAAGT TCCGTACGCA TCGGCACAAG CATCGGTTCA TGCAGAGGCT GAAGCACATG 
AAGACCTGCT TCCATATCCG TCGGCACATG AAGGCCGGAG CGAGTGCCGT GCCGGGTGCA 
GCCAGTGCAG GCGCTGCCAG TGGCGGTGGA ACCGCCGGAG CAATGGGCGG CATGGGTAGC 
TCCCCGGCTA GCTATACAAA CTACCTGGGA CCAATACCCT CGCTACCATC CTCGTGCAGC 
GTCCACGATT TTCACTCTTA CGGATTCCAG GGATCGGGCG TTACACCCGG TATGCACTTC 
CACCTGGCCG CGTCGATAAA CGCGGAAACG GACATCCCGC TGGTGCCATC GATGCAGACC 
AGCGATCCGG CCAATCAGAA TGTCTTTATC CTGCACTGGC TGAACAACAA GGAGATGCAT 
TTCACGCTGC AGGCGGAGGC TATACTCCAG GAGCTCACCA AGAAGGTGAT CGACGAGGAA 
AACGGTCATG GGAATGCCTC CAGCGGCGGC GTCGATCTAC CGGACGATGC CCACGGTTCC 
CATTCGCAGG CACATGCGCA CAAGAAGTAC CTGCGCTCAT CCAAATCGGT GTCCGTGGAC 
GATAGCGGCG AGGAGTTTAG CCGGTATTCG CAGCACACGG CGGGCACGGG AATGGGTGGC 
GGAGGTGGCG GCGCCGGGCT GCATCTGAAC TCCATGTCCA ATACGACGTC ACAGAACTCG 
CTCAACAATG AGCCGGCCCA TCCGATTACG CAGCTAGTGG ATTCGTTGGA CAATAAGATC 
GAGTGTTTGT TGCGCGACTG GCACCAGAGC CCGGATCTGC TCTTCGCCAT TCATCCGGTG 
GACGGGAGCT ACCTGATCTG GGTGATCGAG TGGCTGGATG ACTACTATCC GGGCTCGTTC 
AGGCAGGCGC AGGTCTCCTT CTCCACTCGG ATTCCTTCGG CCTTCCCTCT GGGCGACGCC 
ATGTCCATGT CCACCACGGT CAGCCTCTTC AACACGGGCA CGCATCCGCT GGCCTTCCGC 
GACATAGCGA ACAATGTGCG CAGCAAGGAT GAGTTTCACA AGGGACACGC GGATGATTCC 
TCCCTGAAGA GTGACCAGCA TTCGGGGCAG ACCTTCGAGA GGCACGACGA AGAGCAGGAG 
GATGCTGCCG ATGACGATGG AGATGGAGAT CGCGAAGGCG ACGCAGATCA AGATGAGGGT 
GGTGGCGATC GCAGTGCTGC GGCTAGTGGA GGCGGAGCAG CCGAGCACAG CAGTAGCTCG 
CAAGATTCGG CTGCAACTGG AGCCAGTGCA TTGCCCCTGA ACGTTTCTCC CTCGGTTTCC 
ATGGTGACCA AGCACTCAAA TGGAACCCTT AACCTTTGGC AGCTGACGTT TGCCTTCAAG 
ACGAAGTTTA CCCAAGTATT AAGCATTGGC CACGTAAGTC GTGCCTCCGG CCATCGGTTC 
CGGGTGAACG ACATCACCTG CCACCCGGTG TTACCGCTGC TCGTCTCCAC CTCGCACCAC 
AACCTGCCCG ATTCGGAGGC CAAGTCACCA ATGTCACTGT TTGAGCAACC CCTGAGTGGT 
GGACTTCCCT CGGGCGGCGG GACTGGAGCC GGTCCCTCTG TTGGTTCCAA TGGAGACAAA 
GATGTATTTA CGCCCACGGG CTTTTGTTCG GAGTTAATCC TCTGGCGTGT GGACTCCGTG 
GGACCGCTGT GCCATTCGGG CGGTGTCAGC GAGCTGGCGA GGATTAACTC GCCGGAGATC 
TCGGCCTTCA GCAATGTAGC CTGGATTCCC ACCCTCCTGC CGAGCACCAC ACTGGGCAGC 
TACAGTAACT CCCCGTCCGC CTGCTTCGTG GCCAGCGATG GCGAAAATCT GCGCGTCTAT 
CAGGCAGTGA TCGATGCCCG CACCCTGCTG GCGGAGATCT CCTGCTCGGA GAACCTAAAC 
ACGCTGCACA AATCGCATTC GCTGTCGTCG ATGATCTCGA ATGCTTCGTC TACGATGCGG 
GCCCAGCGAT CGGCGCTGCA CGACAAGCTC AAGGTTGTGT CGCAACAGTC GACGGCGAGA 
CCCGGTTGCA TCTTGCAGCT GGACGCCATC TCGGATGCCA AGCACGACTG GCAAAATACG 
CAGTTCCTGC ACGTGTTCCA GGCGCAGTTG ATAACGGGTG GCCAGAGGAC GCCCCAGGCG 
GATCCCCATG ATTTGGAGGA GCCACGTCTG AACGCTCTGC TCGAGTCGGA CATGGATGCC 
ATCGTCGATT TGCAGCGGAA TGCGGACTTC GAGGAGCCCT TCTACATTGT GAACATCGAG 
AAAACCCTGC GCGGCAGCAC CATACACATG TGGCGCATAG TCATCAGCTC GAAGCAGCAG 
AGCTCGTTCC TTTCCGAGAC GGCCATGTAT GTGCCGGATA GTAATCTTAC CCAGGAGCCA 
GATGAGGATG GCGCCGGTGG TAATCCTAAT CCCAGGCGAA TGTCGCAGGG AGCGGAAACA 
CTGACGGGAG CCGAGCACTT TGGTCCCCAA GTGGAGGCGC CTCACATTTC GATTACCACC 
AAGAAGGTGT GCACTCAAGA GCTGCCACTG CCCGAGGGCG TGGACGTCAT CCATGCCTCT 
CCGGCCGCCG GTCATCTGAG CAGCTCCAGC ATTTACCCAG CCTGCTTTGC GCCGTACATC 
ATTGTGACCG CCTGCTCGGA TTCCATTTCG CGTTTCTGGA AGTGCGAGCC CATCGGTGAT 
GAGGATGATC CGAATCCGGA AGGCGATGCC TATCACTGGA GCGAGTGGAA GATGTTTAGT 
CGCATTCAGC ATTCGGCCAT CGAGATTCCG GGCCAGCCGC TGAACATCAG TGCCGCCTAT 
TCCGGCCGGA TAGCCTGTGC CTACAAGTAC GGCAAGAGTT TTACCCGACC CAATAAGGGA 
CCCGATCCGG ATTCGCGTTA CGTTAACCTC TGCGTGGCCA TCTACGAGTG CGAGAGCTCC 
GGCGGCAGTG AGTGGGTGCT GGAAGACACC ATTCACCTGA AGAATGTCCA TTTGCCGCGA 
ATCAATATTG GTCATGGCAT CGATCTGAGT TACCTGCACG ACAGTCGCCT GCTGGCGAAG 
AAGCAACGGC TGAACCAGGT CCTGCACACC TTCGCACACG ATCCGGAAAG CAGATCGCCG 
CGGAGTGGCG AGACCACACC CGAGTTGGCC GTCAATAGGC AACCGTCGGC CGCTGCATCC 
GGATTGCTAA CCGTGCCCAG CTTCAGTACG CTGCAATCTC TTCGCAAGAG CATAGCCGAA 
AATGGGAACA CCTGTCCGCT GACCCAGAAG CATTTGGTCC AACTGGACTG GGTGAGCAAG 
GAGGATGGTT CACACATCCT TACCGTTGCG GTGGGCAGCA AGATTCTGCT GTACACCCCA 
GTGAGCTCGG ACATTGCCCA GGCGAACATC AAGGCGATGA AGGAGTCGCG TTCAGTGAAT 
CGTCCCATCC TGCGAAAGGC TAGTTCGCTG GCGCAGCCCA ATTTTGTAGA CGAGATCCGT 
TGGATGAAGT TGCGGCAGAT AGACCTCCAG ACCGCCGATG GTCTGCCGCC GCTGCCCATG 
CAGATCTCTT GGGTGCGCGA TGGCATTCTA GTGGTGGCCA TGGACTCCGA GATGCACGTT 
TACTCACAGT GGAAGCCCCA TTATTCCTCG CACTTTGCCG CTGCAGCGGC TGCTGCTGGC 
GAAGATGTAC CGGATACTCG TAACCTGAGG GACGAAGATC TGCGTTCGCT GGCCAACGAG 
TCCACGCAGC TGCGCCTCAA GAACGTGGCG TCCATGCCGC TAATCAGCAA GGTGAGCACG 
GCCAATCTGC AGCTGCTGTC GCACGACAAG AAGCGTCGTC TGGGCAACAC CAGCATGGCC 
AATATGAACG GAGCCGGCGG TGCAGGAGGA ACTGGAACGT ATGGCGGTGG CGCTGATGAC 
GGCGGCAACG ATGACTACAT GACGGACTTC GGTCTGTTCC AGGCCTCTCG CATCGCATGT 
CCAGTGTTGC CGCAATACCA TCCCAAGCAG CTGATGGAGC TGCTTAACTG TGGCAAGATC 
CGTTGGGTGA AGGCCATCCT GGCGCATCTG GTGCGCTGCA TGAGTGGCAA TAGTTCGAGT 
ACAGTGAACC AGGACGATGA TGGCTATGCC AGACAGCGCA GCTGGTCGAG ATCTCGTACC 
CTGAGCATTA GCTACACAGC GGATCAGAAC ATCCAGGCGG AGCATCGCGG CAGCACCACC 
CAAATTCCCG AGGAGCTGAT GCTGGACTAC GCAGAGATCA ATTCGATTCC GCCATTACCC 
TTGTGGGTGC TCCTCAATGC GGATCGTGAG ACGCCGGGTC AGAGCAACAA CTATGCTGAG 
GGCGACAAGG ACTACGACGA GCTCTTTGAC ATGCACAACT CGGAGGACAA CCTGGATGAG 
CTGCTCAGCG AGGGTGATGA GAAGAAGCGC CAGGAGCGCC GTCTCTCTCT GCCCGAGAAG 
CACTCCATTT CGCACTTTGG TCCGCGGCAG GGACAGCTTC TTTCGCGCCT GCTGACCCAC 
ACCCATTTGC CGGGACTCTC CTCGCTGGAC CAGATGCATC TGCTGGCTCT GGCCGATACG 
GTGGCCACGT GCAATACGGA CTTTTCCGAC AAGTTTGCCG CCGATCAGGG CAAGTCGGGC 
GGAATGGCCG GCGGAGCTGG ATCGGGAGCC GTTAAGGACG GTAGCACCAG TACTGATTCC 
CTAGATGATT GCGGTCTACG CTTTCTGCTG GCCATGAAGC ACTTCACCTA CCTGCTGCGA 
TGCCTGCCGC TCCAGCAGAG GGCGCAGTTC CAGCGCCAGG GCGTGGGCAC CTCCAACATC 
GTGTGGGCCT TCCACTCGGA GAGCGAGGAG GAGCTGCTTA ACCTCATTCC TAGCTACACC 
AAAGGCGATC TCCGCTGGGC AACGCTGCGG GATCTGGGCG TCGGCTACTG GCTGAAGAAC 
ATTAACACGC TGAGGAGATG CGTCGAGAAG CTGGCCAAGT GCGCCTACCA GCAGAAGCAG 
GAGCCACTGG ACGCGGCCAT CTATTACCTG GCCATGAAGA AGAAGTCCCT GGTCTGGGGT 
CTGTTCCGTT CACGTCGGGA CGAGAAGATG ACCGCCTTCT TTGGCAACAA TTTTGCCGAG 
GATCGCTGGC GCAAGGCGGC ATTGAAGAAT GCCTTTGTGC TATTGGGCAA GCAGAGATTC 
GAGCACGCGG TGGCCTTCTT CCTGCTGGCC AATAGCCTCA ACGACGCCAT TGAAGTGTGC 
ATGAACAAGC TGGAGGACTT CCAACTGGCC CTGATCATAG CTCGACTCTA TGAGGGCGAC 
GGCGAGGGCT GCTACTACCA CCACTTGCTG CACGAGCACA TTTTGGGCAC GGATGCGGCA 
ACTGGGCGCT GTGATCTCAG TCGCGCGCAT CCCGATCCCT TCTTGCGCTC GATGACCTAC 
TGGACGATCA AGAAGTATCA GGAAAGCTTG AACACCCTGC TCCTGAACAA CGTGGGCAGC 
CTGCACAGCA GCTACAGGGA GGAGGATCTA CTGCGTCCCG AACAACAGGC CACAAATCCC 
AATGTCTTTA ACTTTTACAT CTATTTGCGC ACCCATCCGC TGCTCATCCG GCAGAACATC 
GCTTTGTCGG CGCAGGAGAA GCGCATTGCG CATGTGGTCT TGTCCGGTTT CAATTATGCC 
GGCGATGCTG CTCCCAGTGC CGTGGCCTGT GACAAACAGT TGCAACTGGA GGATTCAATA 
ACGCCCATTG AACGGCAATT GTACTTCACC ACCGCCCATG GGCACTTCAA GAGCGGGTGC 
CCGGCGCTGG CGCTCGAGGT GCTCAACAAG CTGCCCCAGA AGATCAGCGA CGATTCTGGT 
GGCAGTGTGG CCGGCAGCCA GGCGCAGGAG CGTGCCCAGC AGAACAAGGA GGAGCTGATC 
AACACGGGCA TTATGGACCA GTGGGGAGCA ACTCCTGCCG CTGCGTCAAA CACTGCGGAT 
GCCTTCGACT GGGGCGCACC CGTGTCCAGC GAGCCGGAAG CCAAGTTTGA AATAAAATGG 
GATGATGAGG ACGAGGAAGA AGACCCAGAT TTGGAGCCAG ACGAACCGGA GCTAGCCACG 
CCACAGCCGC AGGCCCCAAT TCTTGGCAGA GGCAAATCGA ATGGCAACGA CACAAAGATG 
GACATCATGG CGCAGCAGCT TAAGTTCGTC GCCTGTCTGA AGATTCTTAT GGAGGAGTTG 
TCTACGCTGG CCACCGGTTT TGAGGTGGAT GGCGGCCAGT TGCGCTATCA GCTGTACATG 
TGGCTGGAGC GGGAGGTGGA GGCACTTAAG CAGCTGTGCA ACTACTGCAG TCAAGCGGAG 
CAGTCGAGTC ACGAATCTGG TGATGCTGAT GCGGAGGCCA GGGACAAGGA GATGAACGCC 
AGCATCTATC CCAGCACCGA GCGACCTACG CTTCACGAGA TCCTCATGCA GGACAAGCAA 
GACTTTGAGG CCAAGGTGAT GCGGGCGGCC AAACGCAAGC GATGGCTCAA GGCAAATGAA 
ACCCTACTCC GCACACTGCT GAGTTATTGT TCCCTACACG GCGCCAGTGG TGGTGGCCTG 
GCATCCGTGC GCATGGAGCT GGTACTGCTC CTGCAGGAGC TCCAACAGGA GAAGACCCAA 
CAGCAGCTTC TGAGTCCGCT ACCCTTCCCA ACCACTTTGC CTCTGCTAAG CGCCTGTGTG 
GCGGGCAACA AGACGGTGAT CGCCGATCCC ATCAAGTACC TGCAGTCCCA GACGGTGGAC 
ATGCTGCAGA GCATCATTAA GGTGCTGCCC TTCCCAGGAA TCGAGCCCAG TGCTTTGAGC 
GAGATCTTTG TTTTGCGGGA TCTTGCCGTG GCGCTGTCAT CCTGCATTTA CCAGTCGCTC 
TGCGATTCCG AGAGCTTTGT GGTAAACAAC TCGGCCTTCA ACGGTTACCC CAGTCCGGGA 
ATGGAGAACA TAGCTAAGAT AAACTCATCG TTCGAGTGCA GCTATTTGGT GGGCAGCCGG 
AATGCCTATG GCCGCAGGCG CAAGTACTCT ACGGATGAGC CAGCAGGCGT GTGCACCACG 
CCTTCCAAGT GGCCGGGTGT AACCAATCTC CGTGCGCTTT TGGCGCGGGA AAAGGATGAG 
GATGTGCCTA AGCTAAATGT GCTGCTGCTG GAGTCCTTTG TTTCCACCTA TATGGCGCTC 
TTCATTTACT CGCTATCCAC CTGCGATAGT CGTTTGTTGT ACCGCCTGAG TGGCCAGAGT 
TTCAACAACG AGACCTGGTC CACTCTTTTC GGCGGCGGCA TGAAGAAGTT GCTTATAAAG 
CCCGCTGCTG CACCGCCTCC CACCCAAATG GTGACCGGAG GAGCAGGAGC TGGCGGAGCA 
AGTGGTGGTA CTGGTTCCAC ATCCGAAGAG CCGACCGATG AGAATAGCGT TTGGAACACG 
GTTACTTCCA TTACAAAGCA ACGCATGAAA CTGAACATGA AAATTCTAGG CAGCTTTAGC 
CAGAACAGCA CTTCAAGCAA CATGAAAGAG GACAAGCCGA CATACCGGGA GCAGTTTATG 
CCACCGGAGA CGTCCATGCT ATCTTACTTC CTTACCAAAC CGCCCACAAC CGAATGCGAT 
GACTACGATA CGGACGACTC GCACGAGTCG GACAACGAGG AGGAGGAGGA GGACGACGAT 
GATGTGAATG TGAGTCGATC GCAGCTGAAG GCCAAGGATA ACACGGAGCA CACTAATCCC 
ACGTCGTACT CGTGGAGTAT CCTGCGCCTG GCGCTCATCA AGATCAGCAC GCACAAGATC 
CAGGAGCTGG TCAAGGTGGC CGGCATCGAA CTGCAGGATT TGCCTGTGAT CAGTCCACTC 
TCCCACGAGG TGCTGAGGAC CCTCAATCGC TGGCAGGACT TTGCCCTGAG TGACCTCATA 
GCTCGTGGGC CACCGTCCCG TAATTATATA CCAGGCTGCT ATGCGGAGAG CGGCATCAAC 
GGATTGGCCA TCCACAAGTA CCGCTCGCTC CTGAATAAGG ACAACACACC ATTCGTTTCC 
GGTTCATCGG CTGGACCCAT AAGGCGACTG TGGAACTATT TGGTGCGCCA GGAGCCTGCC 
CAGGAGGTCT TCATCAAGAG CATCTTTGTC AAGAACATGG AGCCCAGCAC GCGAGGATCG 
CACCACACCA ACAACGACAA CGAGACGGAT GAGGGTCAAT CTCATTCGAC CAACGAGAAT 
ACCGACCACA TCCGCATCAT TCACAAGGAC CACGAGTCCA TATTGTCCTT CTGTTTGAAG 
AACAACAGCT CCTCAATGAT TGCCTTTGCG AATCCGCGTG AGATCCAGGA GTTCGACATC 
TCACTGCTGC TGGAGTCGCC GAATTGGTAC GAGGATGAGT GCGACTACGA CATGATGAAC 
TTGTCCAAGG ACGCGGACAC CAACAGCTCG TCCTTCCTGA TCATTCAAAC GCAAGAGCAA 
AACCAATTTG GAAGCAATAG CAATGCCTAC AGCGAGTCCA ATGCTAGCAC ACAAAGTGCT 
TCGCAATCGG GACGCGGCAC ATCCATGGTG CTGCGCCACA AGGTGGACAA TGTGAAGCGC 
ATGAGCGCCC ATCCGCTGAT GCCGCTGTAT CTTACTGGAG GTCAGGACGG ATCCGTGCAG 
ATCTGGGAGT GGGGTCACCA GCAGCCTGTC TGCTCGCCCC GGACTTCGGG AACCTTTGCC 
AAGGTGACGC GCTGCCGCTT CTCCGAGCAG GGCAATAAGT TCGGCATCGG CGATGGCGAC 
GGAAAGCTCA GTCTCTGGCA GGCGGGCATC GCCTCGCAGA ATAATCGTTC GTTTATAAGC 
TACCAGTGCC ACAACAAGGC GTTGTCCGAT TTCGTGTTCC TCGGCTCGTG CAGTCTCCTG 
GCTAGCGCTG GTCAAAGTTC CGAGAACAAG AACATCAACA TCTGGGACAC ATTGCTTCCC 
CACAAAAAGT CCTGTGTATC GGCCTTCACC TGCCACGACC AGGGCTCGTC CTGTCTGGTG 
TTTGCTCCGC AGCACCAGGT GCTCATCTCG TGCGGCAAAC GCGGCGATGT TTGCGTCTTC 
GATGTGAGAC AACGTACGCT GCGTCATCGC TATCAGGCCC ATGATAGCAC CATCAAGTGC 
ATTGCTCTGG ATCCGCACGA GGAGTTCTTT GTCACTGGCT CCATTGAGGG TGACATTAAG 
ATCTGGGACA TGAATCAGTT CATGCTGATC AATACGTTCC CGCATGAGCA TGCCAAGAAC 
GGATTCTTTA AGCACACTGG CCAGGGCGTC AGCCAGGTGT ACGTGGATGC CTTCGGACGG 
CTCTTCTCGT GCGGCTCCGA TGGCTGCATG AAGGTCCGTC TGCTGGTGGA GAAAGACAAC 
ATTGTTCACT CCGTGTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rbcn-3A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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