LOC108134397 cDNA ORF clone, Drosophila bipectinata

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108134397
Entrez Gene ID	108134397
Full Name	voltage-dependent calcium channel type A subunit alpha-1
Gene Type	protein-coding
Organism	Drosophila bipectinata

mRNA and Protein(s)

mRNA	Protein	Name
XM_017254685.1	XP_017110174.1	voltage-dependent calcium channel type A subunit alpha-1 isoform X2
XM_017254684.1	XP_017110173.1	voltage-dependent calcium channel type A subunit alpha-1 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC108134397 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108134397 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODr54994	XM_017254684.1 Latest version!	Drosophila bipectinata voltage-dependent calcium channel type A subunit alpha-1 (LOC108134397), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODr55002	XM_017254685.1 Latest version!	Drosophila bipectinata voltage-dependent calcium channel type A subunit alpha-1 (LOC108134397), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108134397 ( XM_017254684.1 ) cDNA ORF clone, Drosophila bipectinata -> XP_017110173.1 Drosophila bipectinata voltage-dependent calcium channel type A subunit alpha-1 (LOC108134397), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODr54994
Clone ID Related Accession (Same CDS sequence)	XM_017254684.1
Accession Version	XM_017254684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-15
Organism	Drosophila bipectinata
Product	voltage-dependent calcium channel type A subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016088198.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Drosophila bipectinata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCTT TATTGCAAAT CGGTCTCTTG GTGTTGTTTG CAATCGTAAT ATTTGCAATC 
ATTGGACTCG AGTTTTATTC GGGCGCACTG CATAAGACTT GTTATAGCTT AGAAGATCCA 
AACAAACTAG TGAAGGAGGG CGAATCAGAG ACGCCCTGCA ACACAGACAA CGGTACGGAG 
AAGGCAGGTG GCTTTGTTTG CAACAACACC ACTAGCATGT GTCTCGAGAA GTGGGACGGA 
CCAAACCAGG GCATTACGAG TTTCGACAAC ATTGGATTCG CCATGTTAAC GGTGTTCCAA 
TGCATCACCA TGGAGGGCTG GACATCAATA CTCTATTGGA CCAACGACGC ACTAGGTTCA 
ACATTTAATT GGATATATTT CGTGCCTCTT ATAGTTATAG GCTCATTTTT TATGCTCAAC 
CTAGTTCTTG GTGTCTTGAG TGGTGAATTC GCAAAAGAAC GAGAAAAAGT AGAAAATAGA 
CAAGAGTTTC TTAAACTTAG AAGGCAGCAG CAACTAGAAA GAGAGTTAAA CGGCTATGTT 
GAATGGATTT GTAAAGCTGA GGAGGTAATA CTAGCAGAAG AGCGAACCAC AGAAGAAGAA 
AAAATGCACA TTATGGAAGC TCGAAGACGC AATGCTGCCA AGCGGAAAAA GCTCAAAAGT 
TTAGGAAAGT CCAAGTCTAC AGACACAGAG GAAGAGGAGG CCGAGGAAGA TTATGGCGAC 
GATGGTTATT TGAAAACTCG GTCGAAACCC CAAGGCAGCT GCACCGGATT CTGGCGGGCG 
GAAAAGAGAT TCCGCTTTTG GATCCGGCAT ACGGTCAAAA CACAATGGTT CTACTGGTTC 
GTGATCGTCC TTGTCTTCCT GAATACCGTG TGCGTCGCAG TCGAGCACTA CGGCCAGCCA 
TCGTTTCTGA CGGAGTTTCT ATACTATGCG GAGTTCATTT TCCTCGGTTT GTTCATGTCG 
GAGATGTTTA TCAAGATGTA CGCTCTGGGA CCCCGAATCT ACTTTGAGTC ATCATTTAAC 
CGTTTTGATT GCGTTGTCAT TAGTGGTTCG ATATTCGAGG TTATATGGTC CGAGGTCAAA 
GGCGGTTCCT TCGGACTGTC TGTCCTGCGT GCCCTGCGTC TTCTGCGCAT CTTTAAAGTA 
ACCAAGTACT GGTCGTCGCT GCGCAATCTA GTCATCTCCC TGCTGAACTC AATGAGATCC 
ATTATCTCGT TGTTGTTTCT GCTCTTCTTG TTCATACTTA TCTTTGCTCT ACTTGGCATG 
CAGTTGTTTG GCGGCCAGTT TAACTTGCCC GGAGGGACGC CCGAAACCAA TTTTAACACA 
TTCCCGATAG CACTGTTGAC CGTTTTCCAA ATCCTTACCG GCGAAGATTG GAACGAGGTT 
ATGTACCAAG GCATCATATC GCAAGGTGGT GCCCAAAAAG GAATGATTTA CTCTATATAT 
TTTATCGTTT TGGTACTCTT CGGAAATTAC ACGCTATTGA ATGTGTTCTT GGCTATCGCC 
GTTGACAATT TGGCGAATGC CCAAGAACTA ACAGCAGCCG AAGAGGAACA AGTCGAAGAG 
GATAAAGAGA AACAGCTGCA GGAGCTTGAA AAAGAGATGG AGGCGCTCCA GGGTGACGGT 
GTCCACGTAG ACAACGGCGA CGGTACCATC GCCACTACCA AGGCCAAAAA CAAGCGCAAG 
GAAGAGGAGA AGAAGGAGGA GGAGGAAGTG ACTGAAGGTC CCAAGCCAAT GCTGCCATAC 
TCATCAATGT TCATACTTTC TCCAACCAAT CCCATACGAC GCGGCGCCCA TTGGGTTGTT 
AATTTGCCAT ATTTTGATTT CTTTATTATG GTCGTCATAT CAATGTCATC TATAGCATTA 
GCAGCCGAAG ATCCCGTTCG AGAGAACTCA AGGCGAAACA AAATATTAAA TTACTTTGAT 
TATGCATTTA CCGGCGTATT TACCATAGAA ATGTTGCTGA AAATTGTAGA CTTGGGTGTG 
ATCCTGCACC CGGGAAGCTA TTTAAGAGAA TTCTGGAATA TTATGGATGC TGTGGTCGTT 
ATATGCGCTG CTGTTAGTTT CGGTTTCGAT ATGAGCGGTA GCAGCGCTGG ACAAAATTTA 
TCGACAATTA AATCACTTCG AGTATTGCGT GTACTGCGTC CATTAAAGAC TATTAAACGT 
GTTCCAAAAT TGAAAGCCGT CTTCGATTGC GTCGTGAACT CATTGAAAAA TGTTGTTAAC 
ATTCTAATCG TGTACATATT GTTTCAATTT ATATTTTCTG TTATTGGAGT ACAATTGTTC 
AATGGGAAAT TTTTTTATTG TACGGACGAA AGTAAACATA CTTCCGCAGA GTGCCAGGGC 
TCATACTTTA AGTACGAGGA GGACGAATTG CTGCCAAAAC AGGAGCTGAG AGTGTGGAAA 
CCACGAGCCT TTCACTACGA TAACGTTGCC GCCGCGATGC TGACCCTGTT CGCCGTCCAA 
ACCGGCGAGG GATGGCCACA AGTCTTGCAA CACTCTATGG CTGCCACTTA TGAAGATAGG 
GGTCCAATCC AAAATTTCCG GATCGAAATG TCCATATTTT ATATTGTGTA TTTTATTGTG 
TTTCCATTCT TCTTCGTCAA CATATTCGTG GCCTTGATTA TTATTACGTT TCAAGAGCAA 
GGCGAAGCCG AGCTACAAGA CGGTGAAATT GACAAAAATC AGAAATCCTG CATTGACTTT 
ACAATCGGCG CGCGACCCCT TGAGCGATAT ATGCCCAAAA ATCGAAACAC CTTCAAGTAT 
AAAGTTTGGC GCATTGTCGT GTCAACGCCT TTTGAGTACT TTATTATGAT GCTGATCGTA 
TTTAATACCC TCTTACTGAT GATGAAGTAT CATAATCAAG GAGACATGTA CGAAAAGTCG 
CTAAAGTACA TAAACATGGG ATTTACTGGA ATGTTCAGTG TAGAAACTGT GCTAAAAATT 
ATTGGATTCG GCGTCAAGAA CTTCTTCAAG GATCCCTGGA ACATATTTGA TCTGATCACA 
GTCCTTGGCA GCATTGTCGA CGCCTTATGG ATGGAATTTG GGGTAATTAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017110173.1
CDS	55..3108
Translation	MAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNGTEKAGGFVCNNTTSMCLEKWDGPNQGITSFDNIGFAMLTVFQCITMEGWTSILYWTNDALGSTFNWIYFVPLIVIGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIMEARRRNAAKRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVKGGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKGMIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQGDGVHVDNGDGTIATTKAKNKRKEEEKKEEEEVTEGPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGVII

Target ORF information:

RefSeq Version	XM_017254684.1
Organism	Drosophila bipectinata
Definition	Drosophila bipectinata voltage-dependent calcium channel type A subunit alpha-1 (LOC108134397), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017254684.1

ORF Insert Sequence:

ATGGCACCTT TATTGCAAAT CGGTCTCTTG GTGTTGTTTG CAATCGTAAT ATTTGCAATC 
ATTGGACTCG AGTTTTATTC GGGCGCACTG CATAAGACTT GTTATAGCTT AGAAGATCCA 
AACAAACTAG TGAAGGAGGG CGAATCAGAG ACGCCCTGCA ACACAGACAA CGGTACGGAG 
AAGGCAGGTG GCTTTGTTTG CAACAACACC ACTAGCATGT GTCTCGAGAA GTGGGACGGA 
CCAAACCAGG GCATTACGAG TTTCGACAAC ATTGGATTCG CCATGTTAAC GGTGTTCCAA 
TGCATCACCA TGGAGGGCTG GACATCAATA CTCTATTGGA CCAACGACGC ACTAGGTTCA 
ACATTTAATT GGATATATTT CGTGCCTCTT ATAGTTATAG GCTCATTTTT TATGCTCAAC 
CTAGTTCTTG GTGTCTTGAG TGGTGAATTC GCAAAAGAAC GAGAAAAAGT AGAAAATAGA 
CAAGAGTTTC TTAAACTTAG AAGGCAGCAG CAACTAGAAA GAGAGTTAAA CGGCTATGTT 
GAATGGATTT GTAAAGCTGA GGAGGTAATA CTAGCAGAAG AGCGAACCAC AGAAGAAGAA 
AAAATGCACA TTATGGAAGC TCGAAGACGC AATGCTGCCA AGCGGAAAAA GCTCAAAAGT 
TTAGGAAAGT CCAAGTCTAC AGACACAGAG GAAGAGGAGG CCGAGGAAGA TTATGGCGAC 
GATGGTTATT TGAAAACTCG GTCGAAACCC CAAGGCAGCT GCACCGGATT CTGGCGGGCG 
GAAAAGAGAT TCCGCTTTTG GATCCGGCAT ACGGTCAAAA CACAATGGTT CTACTGGTTC 
GTGATCGTCC TTGTCTTCCT GAATACCGTG TGCGTCGCAG TCGAGCACTA CGGCCAGCCA 
TCGTTTCTGA CGGAGTTTCT ATACTATGCG GAGTTCATTT TCCTCGGTTT GTTCATGTCG 
GAGATGTTTA TCAAGATGTA CGCTCTGGGA CCCCGAATCT ACTTTGAGTC ATCATTTAAC 
CGTTTTGATT GCGTTGTCAT TAGTGGTTCG ATATTCGAGG TTATATGGTC CGAGGTCAAA 
GGCGGTTCCT TCGGACTGTC TGTCCTGCGT GCCCTGCGTC TTCTGCGCAT CTTTAAAGTA 
ACCAAGTACT GGTCGTCGCT GCGCAATCTA GTCATCTCCC TGCTGAACTC AATGAGATCC 
ATTATCTCGT TGTTGTTTCT GCTCTTCTTG TTCATACTTA TCTTTGCTCT ACTTGGCATG 
CAGTTGTTTG GCGGCCAGTT TAACTTGCCC GGAGGGACGC CCGAAACCAA TTTTAACACA 
TTCCCGATAG CACTGTTGAC CGTTTTCCAA ATCCTTACCG GCGAAGATTG GAACGAGGTT 
ATGTACCAAG GCATCATATC GCAAGGTGGT GCCCAAAAAG GAATGATTTA CTCTATATAT 
TTTATCGTTT TGGTACTCTT CGGAAATTAC ACGCTATTGA ATGTGTTCTT GGCTATCGCC 
GTTGACAATT TGGCGAATGC CCAAGAACTA ACAGCAGCCG AAGAGGAACA AGTCGAAGAG 
GATAAAGAGA AACAGCTGCA GGAGCTTGAA AAAGAGATGG AGGCGCTCCA GGGTGACGGT 
GTCCACGTAG ACAACGGCGA CGGTACCATC GCCACTACCA AGGCCAAAAA CAAGCGCAAG 
GAAGAGGAGA AGAAGGAGGA GGAGGAAGTG ACTGAAGGTC CCAAGCCAAT GCTGCCATAC 
TCATCAATGT TCATACTTTC TCCAACCAAT CCCATACGAC GCGGCGCCCA TTGGGTTGTT 
AATTTGCCAT ATTTTGATTT CTTTATTATG GTCGTCATAT CAATGTCATC TATAGCATTA 
GCAGCCGAAG ATCCCGTTCG AGAGAACTCA AGGCGAAACA AAATATTAAA TTACTTTGAT 
TATGCATTTA CCGGCGTATT TACCATAGAA ATGTTGCTGA AAATTGTAGA CTTGGGTGTG 
ATCCTGCACC CGGGAAGCTA TTTAAGAGAA TTCTGGAATA TTATGGATGC TGTGGTCGTT 
ATATGCGCTG CTGTTAGTTT CGGTTTCGAT ATGAGCGGTA GCAGCGCTGG ACAAAATTTA 
TCGACAATTA AATCACTTCG AGTATTGCGT GTACTGCGTC CATTAAAGAC TATTAAACGT 
GTTCCAAAAT TGAAAGCCGT CTTCGATTGC GTCGTGAACT CATTGAAAAA TGTTGTTAAC 
ATTCTAATCG TGTACATATT GTTTCAATTT ATATTTTCTG TTATTGGAGT ACAATTGTTC 
AATGGGAAAT TTTTTTATTG TACGGACGAA AGTAAACATA CTTCCGCAGA GTGCCAGGGC 
TCATACTTTA AGTACGAGGA GGACGAATTG CTGCCAAAAC AGGAGCTGAG AGTGTGGAAA 
CCACGAGCCT TTCACTACGA TAACGTTGCC GCCGCGATGC TGACCCTGTT CGCCGTCCAA 
ACCGGCGAGG GATGGCCACA AGTCTTGCAA CACTCTATGG CTGCCACTTA TGAAGATAGG 
GGTCCAATCC AAAATTTCCG GATCGAAATG TCCATATTTT ATATTGTGTA TTTTATTGTG 
TTTCCATTCT TCTTCGTCAA CATATTCGTG GCCTTGATTA TTATTACGTT TCAAGAGCAA 
GGCGAAGCCG AGCTACAAGA CGGTGAAATT GACAAAAATC AGAAATCCTG CATTGACTTT 
ACAATCGGCG CGCGACCCCT TGAGCGATAT ATGCCCAAAA ATCGAAACAC CTTCAAGTAT 
AAAGTTTGGC GCATTGTCGT GTCAACGCCT TTTGAGTACT TTATTATGAT GCTGATCGTA 
TTTAATACCC TCTTACTGAT GATGAAGTAT CATAATCAAG GAGACATGTA CGAAAAGTCG 
CTAAAGTACA TAAACATGGG ATTTACTGGA ATGTTCAGTG TAGAAACTGT GCTAAAAATT 
ATTGGATTCG GCGTCAAGAA CTTCTTCAAG GATCCCTGGA ACATATTTGA TCTGATCACA 
GTCCTTGGCA GCATTGTCGA CGCCTTATGG ATGGAATTTG GGGTAATTAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108134397 ( XM_017254685.1 ) cDNA ORF clone, Drosophila bipectinata -> XP_017110174.1 Drosophila bipectinata voltage-dependent calcium channel type A subunit alpha-1 (LOC108134397), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODr55002
Clone ID Related Accession (Same CDS sequence)	XM_017254685.1
Accession Version	XM_017254685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-15
Organism	Drosophila bipectinata
Product	voltage-dependent calcium channel type A subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016088198.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Drosophila bipectinata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGCC CAAAAAAAGA GGAGAACCCA CCAGGTGGTG GTCCGACGTC GTTATTTATC 
CTTACTGAGG ATAACCCAAT TAGGAAGTAC ACACGATTCA TCATAGAATG GCCGCCCTTT 
GAATACGCTG TGTTATTGAC AATCATAGCC AACTGTGTTG TGTTGGCACT GGAGGAGCAC 
TTGCCCGGGG GCGACAAGAC AGTGTTGGCT CAAAAATTGG AAAAAACGGA GGCCTATTTT 
TTATGCATTT TCTGTGTAGA AGCATCGCTC AAGATCCTCG CCTTAGGGCT TGTTCTGCAT 
AAACACTCCT ATCTCAGGAA TATTTGGAAC ATCATGGATT TTTTCGTTGT AGTGACGGGA 
TTCATGACAC AGTACCCACA AATAGGGCCC GAGGTAGACC TAAGAACACT TAGAGCCATT 
CGTGTGCTAC GGCCCCTAAA ATTAGTGTCT GGAATTCCTA GTTTACAAGT AGTTTTAAAA 
TCTATTATCA AGGCGATGGC ACCTTTATTG CAAATCGGTC TCTTGGTGTT GTTTGCAATC 
GTAATATTTG CAATCATTGG ACTCGAGTTT TATTCGGGCG CACTGCATAA GACTTGTTAT 
AGCTTAGAAG ATCCAAACAA ACTAGTGAAG GAGGGCGAAT CAGAGACGCC CTGCAACACA 
GACAACGGTA CGGAGAAGGC AGGTGGCTTT GTTTGCAACA ACACCACTAG CATGTGTCTC 
GAGAAGTGGG ACGGACCAAA CCAGGGCATT ACGAGTTTCG ACAACATTGG ATTCGCCATG 
TTAACGGTGT TCCAATGCAT CACCATGGAG GGCTGGACAT CAATACTCTA TTGGACCAAC 
GACGCACTAG GTTCAACATT TAATTGGATA TATTTCGTGC CTCTTATAGT TATAGGCTCA 
TTTTTTATGC TCAACCTAGT TCTTGGTGTC TTGAGTGGTG AATTCGCAAA AGAACGAGAA 
AAAGTAGAAA ATAGACAAGA GTTTCTTAAA CTTAGAAGGC AGCAGCAACT AGAAAGAGAG 
TTAAACGGCT ATGTTGAATG GATTTGTAAA GCTGAGGAGG TAATACTAGC AGAAGAGCGA 
ACCACAGAAG AAGAAAAAAT GCACATTATG GAAGCTCGAA GACGCAATGC TGCCAAGCGG 
AAAAAGCTCA AAAGTTTAGG AAAGTCCAAG TCTACAGACA CAGAGGAAGA GGAGGCCGAG 
GAAGATTATG GCGACGATGG TTATTTGAAA ACTCGGTCGA AACCCCAAGG CAGCTGCACC 
GGATTCTGGC GGGCGGAAAA GAGATTCCGC TTTTGGATCC GGCATACGGT CAAAACACAA 
TGGTTCTACT GGTTCGTGAT CGTCCTTGTC TTCCTGAATA CCGTGTGCGT CGCAGTCGAG 
CACTACGGCC AGCCATCGTT TCTGACGGAG TTTCTATACT ATGCGGAGTT CATTTTCCTC 
GGTTTGTTCA TGTCGGAGAT GTTTATCAAG ATGTACGCTC TGGGACCCCG AATCTACTTT 
GAGTCATCAT TTAACCGTTT TGATTGCGTT GTCATTAGTG GTTCGATATT CGAGGTTATA 
TGGTCCGAGG TCAAAGGCGG TTCCTTCGGA CTGTCTGTCC TGCGTGCCCT GCGTCTTCTG 
CGCATCTTTA AAGTAACCAA GTACTGGTCG TCGCTGCGCA ATCTAGTCAT CTCCCTGCTG 
AACTCAATGA GATCCATTAT CTCGTTGTTG TTTCTGCTCT TCTTGTTCAT ACTTATCTTT 
GCTCTACTTG GCATGCAGTT GTTTGGCGGC CAGTTTAACT TGCCCGGAGG GACGCCCGAA 
ACCAATTTTA ACACATTCCC GATAGCACTG TTGACCGTTT TCCAAATCCT TACCGGCGAA 
GATTGGAACG AGGTTATGTA CCAAGGCATC ATATCGCAAG GTGGTGCCCA AAAAGGAATG 
ATTTACTCTA TATATTTTAT CGTTTTGGTA CTCTTCGGAA ATTACACGCT ATTGAATGTG 
TTCTTGGCTA TCGCCGTTGA CAATTTGGCG AATGCCCAAG AACTAACAGC AGCCGAAGAG 
GAACAAGTCG AAGAGGATAA AGAGAAACAG CTGCAGGAGC TTGAAAAAGA GATGGAGGCG 
CTCCAGGGTG ACGGTGTCCA CGTAGACAAC GGCGACGGTA CCATCGCCAC TACCAAGGCC 
AAAAACAAGC GCAAGGAAGA GGAGAAGAAG GAGGAGGAGG AAGTGACTGA AGGTCCCAAG 
CCAATGCTGC CATACTCATC AATGTTCATA CTTTCTCCAA CCAATCCCAT ACGACGCGGC 
GCCCATTGGG TTGTTAATTT GCCATATTTT GATTTCTTTA TTATGGTCGT CATATCAATG 
TCATCTATAG CATTAGCAGC CGAAGATCCC GTTCGAGAGA ACTCAAGGCG AAACAAAATA 
TTAAATTACT TTGATTATGC ATTTACCGGC GTATTTACCA TAGAAATGTT GCTGAAAATT 
GTAGACTTGG GTGTGATCCT GCACCCGGGA AGCTATTTAA GAGAATTCTG GAATATTATG 
GATGCTGTGG TCGTTATATG CGCTGCTGTT AGTTTCGGTT TCGATATGAG CGGTAGCAGC 
GCTGGACAAA ATTTATCGAC AATTAAATCA CTTCGAGTAT TGCGTGTACT GCGTCCATTA 
AAGACTATTA AACGTGTTCC AAAATTGAAA GCCGTCTTCG ATTGCGTCGT GAACTCATTG 
AAAAATGTTG TTAACATTCT AATCGTGTAC ATATTGTTTC AATTTATATT TTCTGTTATT 
GGAGTACAAT TGTTCAATGG GAAATTTTTT TATTGTACGG ACGAAAGTAA ACATACTTCC 
GCAGAGTGCC AGGGCTCATA CTTTAAGTAC GAGGAGGACG AATTGCTGCC AAAACAGGAG 
CTGAGAGTGT GGAAACCACG AGCCTTTCAC TACGATAACG TTGCCGCCGC GATGCTGACC 
CTGTTCGCCG TCCAAACCGG CGAGGGATGG CCACAAGTCT TGCAACACTC TATGGCTGCC 
ACTTATGAAG ATAGGGGTCC AATCCAAAAT TTCCGGATCG AAATGTCCAT ATTTTATATT 
GTGTATTTTA TTGTGTTTCC ATTCTTCTTC GTCAACATAT TCGTGGCCTT GATTATTATT 
ACGTTTCAAG AGCAAGGCGA AGCCGAGCTA CAAGACGGTG AAATTGACAA AAATCAGAAA 
TCCTGCATTG ACTTTACAAT CGGCGCGCGA CCCCTTGAGC GATATATGCC CAAAAATCGA 
AACACCTTCA AGTATAAAGT TTGGCGCATT GTCGTGTCAA CGCCTTTTGA GTACTTTATT 
ATGATGCTGA TCGTATTTAA TACCCTCTTA CTGATGATGA AGTATCATAA TCAAGGAGAC 
ATGTACGAAA AGTCGCTAAA GTACATAAAC ATGGGATTTA CTGGAATGTT CAGTGTAGAA 
ACTGTGCTAA AAATTATTGG ATTCGGCGTC AAGAACTTCT TCAAGGATCC CTGGAACATA 
TTTGATCTGA TCACAGTCCT TGGCAGCATT GTCGACGCCT TATGGATGGA ATTTGGGGTA 
ATTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017110174.1
CDS	96..3644
Translation	MGGPKKEENPPGGGPTSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNGTEKAGGFVCNNTTSMCLEKWDGPNQGITSFDNIGFAMLTVFQCITMEGWTSILYWTNDALGSTFNWIYFVPLIVIGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIMEARRRNAAKRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVKGGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKGMIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQGDGVHVDNGDGTIATTKAKNKRKEEEKKEEEEVTEGPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGVII

Target ORF information:

RefSeq Version	XM_017254685.1
Organism	Drosophila bipectinata
Definition	Drosophila bipectinata voltage-dependent calcium channel type A subunit alpha-1 (LOC108134397), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017254685.1

ORF Insert Sequence:

ATGGGTGGCC CAAAAAAAGA GGAGAACCCA CCAGGTGGTG GTCCGACGTC GTTATTTATC 
CTTACTGAGG ATAACCCAAT TAGGAAGTAC ACACGATTCA TCATAGAATG GCCGCCCTTT 
GAATACGCTG TGTTATTGAC AATCATAGCC AACTGTGTTG TGTTGGCACT GGAGGAGCAC 
TTGCCCGGGG GCGACAAGAC AGTGTTGGCT CAAAAATTGG AAAAAACGGA GGCCTATTTT 
TTATGCATTT TCTGTGTAGA AGCATCGCTC AAGATCCTCG CCTTAGGGCT TGTTCTGCAT 
AAACACTCCT ATCTCAGGAA TATTTGGAAC ATCATGGATT TTTTCGTTGT AGTGACGGGA 
TTCATGACAC AGTACCCACA AATAGGGCCC GAGGTAGACC TAAGAACACT TAGAGCCATT 
CGTGTGCTAC GGCCCCTAAA ATTAGTGTCT GGAATTCCTA GTTTACAAGT AGTTTTAAAA 
TCTATTATCA AGGCGATGGC ACCTTTATTG CAAATCGGTC TCTTGGTGTT GTTTGCAATC 
GTAATATTTG CAATCATTGG ACTCGAGTTT TATTCGGGCG CACTGCATAA GACTTGTTAT 
AGCTTAGAAG ATCCAAACAA ACTAGTGAAG GAGGGCGAAT CAGAGACGCC CTGCAACACA 
GACAACGGTA CGGAGAAGGC AGGTGGCTTT GTTTGCAACA ACACCACTAG CATGTGTCTC 
GAGAAGTGGG ACGGACCAAA CCAGGGCATT ACGAGTTTCG ACAACATTGG ATTCGCCATG 
TTAACGGTGT TCCAATGCAT CACCATGGAG GGCTGGACAT CAATACTCTA TTGGACCAAC 
GACGCACTAG GTTCAACATT TAATTGGATA TATTTCGTGC CTCTTATAGT TATAGGCTCA 
TTTTTTATGC TCAACCTAGT TCTTGGTGTC TTGAGTGGTG AATTCGCAAA AGAACGAGAA 
AAAGTAGAAA ATAGACAAGA GTTTCTTAAA CTTAGAAGGC AGCAGCAACT AGAAAGAGAG 
TTAAACGGCT ATGTTGAATG GATTTGTAAA GCTGAGGAGG TAATACTAGC AGAAGAGCGA 
ACCACAGAAG AAGAAAAAAT GCACATTATG GAAGCTCGAA GACGCAATGC TGCCAAGCGG 
AAAAAGCTCA AAAGTTTAGG AAAGTCCAAG TCTACAGACA CAGAGGAAGA GGAGGCCGAG 
GAAGATTATG GCGACGATGG TTATTTGAAA ACTCGGTCGA AACCCCAAGG CAGCTGCACC 
GGATTCTGGC GGGCGGAAAA GAGATTCCGC TTTTGGATCC GGCATACGGT CAAAACACAA 
TGGTTCTACT GGTTCGTGAT CGTCCTTGTC TTCCTGAATA CCGTGTGCGT CGCAGTCGAG 
CACTACGGCC AGCCATCGTT TCTGACGGAG TTTCTATACT ATGCGGAGTT CATTTTCCTC 
GGTTTGTTCA TGTCGGAGAT GTTTATCAAG ATGTACGCTC TGGGACCCCG AATCTACTTT 
GAGTCATCAT TTAACCGTTT TGATTGCGTT GTCATTAGTG GTTCGATATT CGAGGTTATA 
TGGTCCGAGG TCAAAGGCGG TTCCTTCGGA CTGTCTGTCC TGCGTGCCCT GCGTCTTCTG 
CGCATCTTTA AAGTAACCAA GTACTGGTCG TCGCTGCGCA ATCTAGTCAT CTCCCTGCTG 
AACTCAATGA GATCCATTAT CTCGTTGTTG TTTCTGCTCT TCTTGTTCAT ACTTATCTTT 
GCTCTACTTG GCATGCAGTT GTTTGGCGGC CAGTTTAACT TGCCCGGAGG GACGCCCGAA 
ACCAATTTTA ACACATTCCC GATAGCACTG TTGACCGTTT TCCAAATCCT TACCGGCGAA 
GATTGGAACG AGGTTATGTA CCAAGGCATC ATATCGCAAG GTGGTGCCCA AAAAGGAATG 
ATTTACTCTA TATATTTTAT CGTTTTGGTA CTCTTCGGAA ATTACACGCT ATTGAATGTG 
TTCTTGGCTA TCGCCGTTGA CAATTTGGCG AATGCCCAAG AACTAACAGC AGCCGAAGAG 
GAACAAGTCG AAGAGGATAA AGAGAAACAG CTGCAGGAGC TTGAAAAAGA GATGGAGGCG 
CTCCAGGGTG ACGGTGTCCA CGTAGACAAC GGCGACGGTA CCATCGCCAC TACCAAGGCC 
AAAAACAAGC GCAAGGAAGA GGAGAAGAAG GAGGAGGAGG AAGTGACTGA AGGTCCCAAG 
CCAATGCTGC CATACTCATC AATGTTCATA CTTTCTCCAA CCAATCCCAT ACGACGCGGC 
GCCCATTGGG TTGTTAATTT GCCATATTTT GATTTCTTTA TTATGGTCGT CATATCAATG 
TCATCTATAG CATTAGCAGC CGAAGATCCC GTTCGAGAGA ACTCAAGGCG AAACAAAATA 
TTAAATTACT TTGATTATGC ATTTACCGGC GTATTTACCA TAGAAATGTT GCTGAAAATT 
GTAGACTTGG GTGTGATCCT GCACCCGGGA AGCTATTTAA GAGAATTCTG GAATATTATG 
GATGCTGTGG TCGTTATATG CGCTGCTGTT AGTTTCGGTT TCGATATGAG CGGTAGCAGC 
GCTGGACAAA ATTTATCGAC AATTAAATCA CTTCGAGTAT TGCGTGTACT GCGTCCATTA 
AAGACTATTA AACGTGTTCC AAAATTGAAA GCCGTCTTCG ATTGCGTCGT GAACTCATTG 
AAAAATGTTG TTAACATTCT AATCGTGTAC ATATTGTTTC AATTTATATT TTCTGTTATT 
GGAGTACAAT TGTTCAATGG GAAATTTTTT TATTGTACGG ACGAAAGTAA ACATACTTCC 
GCAGAGTGCC AGGGCTCATA CTTTAAGTAC GAGGAGGACG AATTGCTGCC AAAACAGGAG 
CTGAGAGTGT GGAAACCACG AGCCTTTCAC TACGATAACG TTGCCGCCGC GATGCTGACC 
CTGTTCGCCG TCCAAACCGG CGAGGGATGG CCACAAGTCT TGCAACACTC TATGGCTGCC 
ACTTATGAAG ATAGGGGTCC AATCCAAAAT TTCCGGATCG AAATGTCCAT ATTTTATATT 
GTGTATTTTA TTGTGTTTCC ATTCTTCTTC GTCAACATAT TCGTGGCCTT GATTATTATT 
ACGTTTCAAG AGCAAGGCGA AGCCGAGCTA CAAGACGGTG AAATTGACAA AAATCAGAAA 
TCCTGCATTG ACTTTACAAT CGGCGCGCGA CCCCTTGAGC GATATATGCC CAAAAATCGA 
AACACCTTCA AGTATAAAGT TTGGCGCATT GTCGTGTCAA CGCCTTTTGA GTACTTTATT 
ATGATGCTGA TCGTATTTAA TACCCTCTTA CTGATGATGA AGTATCATAA TCAAGGAGAC 
ATGTACGAAA AGTCGCTAAA GTACATAAAC ATGGGATTTA CTGGAATGTT CAGTGTAGAA 
ACTGTGCTAA AAATTATTGG ATTCGGCGTC AAGAACTTCT TCAAGGATCC CTGGAACATA 
TTTGATCTGA TCACAGTCCT TGGCAGCATT GTCGACGCCT TATGGATGGA ATTTGGGGTA 
ATTATTTGA