LOC109538536 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109538536
Entrez Gene ID	109538536
Full Name	cysteine protease ATG4B
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019905796.1	XP_019761355.1	cysteine protease ATG4B

Pathways from BioSystems
Gene Ontology

The following LOC109538536 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109538536 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe42279	XM_019905796.1 Latest version!	Dendroctonus ponderosae cysteine protease ATG4B (LOC109538536), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109538536 ( XM_019905796.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019761355.1 Dendroctonus ponderosae cysteine protease ATG4B (LOC109538536), mRNA.

Price & Availability↑

CloneID	ODe42279
Clone ID Related Accession (Same CDS sequence)	XM_019905796.1
Accession Version	XM_019905796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	cysteine protease ATG4B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852073.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAACG CAACAAGGGA CATAATGGAT TGCATGTTCG AGGCTGTGCT CGACTCCACT 
CAAGACCCCG ATGACATTCC CCAGAGCACT GAGCCCGTTT GGCTCTTAGG AAAGAAATAT 
CATGCAATTA ATGAACTAAA CACCATAAGG CAAGACATCG TATCAAAGCT ATGGTTTACA 
TATAGAAAAG ATTTTGTCCC CATCGGGGGC AGCGATGGGA AAACTTCAGA TAAAGGCTGG 
GGCTGTATGC TGAGGTGTGG CCAAATGGTG CTAGGACAGG CTTTGATGAG TATACATTTA 
GGCAGAGATT GGCAATGGAA TCCCACCACC AGAGATGCAA CGTATTTAAG TATTTTAAAG 
AAGTTTGAAG ACTCTAGGAA AGCCCCATTT TCAATTCACC AGATCGCTAG CATGGGGATA 
TCGGAAGGGA AGGAAGTTGG GCAGTGGTTT GGCCCCAACA CTGTTGCCCA AGTTTTGAAA 
AAATTAGTAA AATTCGATGA AGGCAACGAC GTTGCCATTC ACGTAGCTCT GGACAATGTA 
GTTATTATAA GTGAAATTAG AGACCTTTGC CTCAGTAAAG AAACAGCGGA TGTTTCCACA 
CCGCATTGGA AACCACTCTT GCTTATCGTG CCCTTAAGAT TGGGCCTCAC GCAGATGAAC 
AGCATTTACT TGGGAGGCCT AAAGCAATGT TTTCAATTCA AACAAAGTTT AGGGATAATA 
GGCGGCAAAC CAAACTCGGC CCTCTATTTT ATCGGTTACG TTGGAAATGA GGTCATCTAT 
TTCGATCCAC ATACAACGCA AAAAGCTGGA TCAGTAGGGA ACAAAGACAC CAGCGAAGAA 
AAGGACGTTG ATCTGAGCTA CCATTGTAAA CATGCTTCTA GAATGAGTAT GCTAGGCATG 
GATCCCTCAG TCGCTGTGTG CTTCCTTTGC AGATCTGAGG CCGATTTCAA CGACCTTTGC 
CAAAACATCA AGGACCAACT GATAAAGACT GAGTCGCAGC CTCTCTTCGA GGTTTCGTTT 
GAGAGAACCG AGGAGTGGCT GTCTACCGTT GAAGAGCTAA ACCTGTCGGG TCTAAGCGAC 
CAGTCCGATC AAGAGGAATT TGAGATTTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019761355.1
CDS	156..1268
Translation	MCNATRDIMDCMFEAVLDSTQDPDDIPQSTEPVWLLGKKYHAINELNTIRQDIVSKLWFTYRKDFVPIGGSDGKTSDKGWGCMLRCGQMVLGQALMSIHLGRDWQWNPTTRDATYLSILKKFEDSRKAPFSIHQIASMGISEGKEVGQWFGPNTVAQVLKKLVKFDEGNDVAIHVALDNVVIISEIRDLCLSKETADVSTPHWKPLLLIVPLRLGLTQMNSIYLGGLKQCFQFKQSLGIIGGKPNSALYFIGYVGNEVIYFDPHTTQKAGSVGNKDTSEEKDVDLSYHCKHASRMSMLGMDPSVAVCFLCRSEADFNDLCQNIKDQLIKTESQPLFEVSFERTEEWLSTVEELNLSGLSDQSDQEEFEIL

Target ORF information:

RefSeq Version	XM_019905796.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae cysteine protease ATG4B (LOC109538536), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019905796.1

ORF Insert Sequence:

ATGTGCAACG CAACAAGGGA CATAATGGAT TGCATGTTCG AGGCTGTGCT CGACTCCACT 
CAAGACCCCG ATGACATTCC CCAGAGCACT GAGCCCGTTT GGCTCTTAGG AAAGAAATAT 
CATGCAATTA ATGAACTAAA CACCATAAGG CAAGACATCG TATCAAAGCT ATGGTTTACA 
TATAGAAAAG ATTTTGTCCC CATCGGGGGC AGCGATGGGA AAACTTCAGA TAAAGGCTGG 
GGCTGTATGC TGAGGTGTGG CCAAATGGTG CTAGGACAGG CTTTGATGAG TATACATTTA 
GGCAGAGATT GGCAATGGAA TCCCACCACC AGAGATGCAA CGTATTTAAG TATTTTAAAG 
AAGTTTGAAG ACTCTAGGAA AGCCCCATTT TCAATTCACC AGATCGCTAG CATGGGGATA 
TCGGAAGGGA AGGAAGTTGG GCAGTGGTTT GGCCCCAACA CTGTTGCCCA AGTTTTGAAA 
AAATTAGTAA AATTCGATGA AGGCAACGAC GTTGCCATTC ACGTAGCTCT GGACAATGTA 
GTTATTATAA GTGAAATTAG AGACCTTTGC CTCAGTAAAG AAACAGCGGA TGTTTCCACA 
CCGCATTGGA AACCACTCTT GCTTATCGTG CCCTTAAGAT TGGGCCTCAC GCAGATGAAC 
AGCATTTACT TGGGAGGCCT AAAGCAATGT TTTCAATTCA AACAAAGTTT AGGGATAATA 
GGCGGCAAAC CAAACTCGGC CCTCTATTTT ATCGGTTACG TTGGAAATGA GGTCATCTAT 
TTCGATCCAC ATACAACGCA AAAAGCTGGA TCAGTAGGGA ACAAAGACAC CAGCGAAGAA 
AAGGACGTTG ATCTGAGCTA CCATTGTAAA CATGCTTCTA GAATGAGTAT GCTAGGCATG 
GATCCCTCAG TCGCTGTGTG CTTCCTTTGC AGATCTGAGG CCGATTTCAA CGACCTTTGC 
CAAAACATCA AGGACCAACT GATAAAGACT GAGTCGCAGC CTCTCTTCGA GGTTTCGTTT 
GAGAGAACCG AGGAGTGGCT GTCTACCGTT GAAGAGCTAA ACCTGTCGGG TCTAAGCGAC 
CAGTCCGATC AAGAGGAATT TGAGATTTTA TAG