The following SPNS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPNS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ODa08104 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005163832.4 , NM_153663.2 , XM_005163832.3 | |
| Accession Version | NM_153663.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-06-30 | |
| Organism | Danio rerio(zebrafish) | |
| Product | protein spinster homolog 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH595239.1, CK029141.1, EH585608.1, CK027241.1, BX784026.17, CF999294.1 and EH478886.1. On Sep 3, 2015 this sequence version replaced NM_153663.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF465772.1, BC048024.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_705949.2 |
| CDS | 241..1755 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_153663.2 |
1 | ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa08104 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005163832.4 , NM_153663.2 , XM_005163832.3 | |
| Accession Version | XM_005163832.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | protein spinster homolog 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163832.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005163889.1 |
| CDS | 255..1769 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005163832.4 |
1 | ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa08104 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005163832.4 , NM_153663.2 , XM_005163832.3 | |
| Accession Version | XM_005163832.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-23 | |
| Organism | Danio rerio(Zebrafish) | |
| Product | protein spinster homolog 1 isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005163889.1 |
| CDS | 253..1767 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005163832.3 |
1 | ATGTCACAAG CAGATGCAGA CATCACGCCC TTCTTCGCTG ATGATAATGA AGGGGAGGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Autolysosome biogenesis and developmental senescence are regulated by both Spns1 and v-ATPase. |
Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database. |
Aberrant autolysosomal regulation is linked to the induction of embryonic senescence: differential roles of Beclin 1 and p53 in vertebrate Spns1 deficiency. |
The zebrafish gene map defines ancestral vertebrate chromosomes. |
Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis. |
