il10ra cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	il10ra
Entrez Gene ID	777651
Full Name	interleukin 10 receptor, alpha
Synonyms	crfb7
General protein information	Preferred Names interleukin 10 receptor, alpha Names interleukin 10 receptor, alpha IL10R1 cytokine receptor family member b7
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077625.1	NP_001071093.1	interleukin 10 receptor, alpha precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Polystyrene microplastics induce microbiota dysbiosis and inflammation in the gut of adult zebrafish.
Jin Y, Xia J, Pan Z, Yang J, Wang W, Fu Z
Environmental pollution (Barking, Essex : 1987)235322-329(2018 Jan)

Cloning, identification of the two cytokine receptor family B subunits CRFB1 and CRFB5 from grass carp (Ctenopharyngodon idella).
Chen H, Liu W, Wang B, Mao H, Sun Z, Hou Q, Mi Y, Fan L, Hu C
Fish & shellfish immunology45(2)211-20(2015 Aug)

Koi herpesvirus encodes and expresses a functional interleukin-10.
Sunarto A, Liongue C, McColl KA, Adams MM, Bulach D, Crane MS, Schat KA, Slobedman B, Barnes AC, Ward AC, Walker PJ
Journal of virology86(21)11512-20(2012 Nov)

Identification and molecular characterization of the interleukin-10 receptor 1 of the zebrafish (Danio rerio) and the goldfish (Carassius auratus L.).
Grayfer L, Belosevic M
Developmental and comparative immunology36(2)408-17(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following il10ra gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il10ra cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33362	NM_001077625.1 Latest version!	Danio rerio interleukin 10 receptor, alpha (il10ra), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il10ra ( NM_001077625.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001071093.1 Danio rerio interleukin 10 receptor, alpha (il10ra), mRNA.

Price & Availability↑

CloneID	ODa33362
Clone ID Related Accession (Same CDS sequence)	NM_001077625.1
Accession Version	NM_001077625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	interleukin 10 receptor, alpha precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF014957.1. ##Evidence-Data-START## Transcript exon combination :: EF014957.1, BC142763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGATTGGA CCTTGTGGAT TTGTTTTCTA GTCTGCTGTA CCCCAGGATT GATAGCAGGT 
GAGCAGAAGA AATTAAATCT GACGTTCGAC ATATGGGAGG GAAATGTAAC TGTTCTGTGG 
GATCCCCCCA ATAAAGCCCC CGTACCTGCC TTTTATCAAG TAAAACTAGC ACTAGACACT 
GGTCCTCATG CTAAATGGAA CATTGTGCAA GAATGTGACA TGATTACAGA AACTAAATGT 
AATCTTGGAA ACTTTATATT CAAACCTACA ATGAAAGTAA AAATTGAACT GATAAGTGGA 
AATAACGAAT CTTCGTGGTT GGTGATAAGA AGGATTCGTT TAGATTATAT TAAGCTGTTG 
TCTCCAGACT TTGACCTGTT CTCAGGGCCA AATACGGTGA AGGTTAAGAT TCACAGAAAG 
CCATTTCTCA AAGAGCTTTT TCCATATGGA CTAAGTTACT CTGCCATTCT GTATCCGAGA 
GGACAGGAGA GTAAGGCTAA AACATTGAGT GATGATGATG ATGAAGATGG TGAAGTGGAG 
TTTACTGATT TATCATATTG GCAAACGTAT TGTGTTCGTG TGAGATTGGA GATCACATCT 
ATTGCAGTGA GCAACACCTC ATTGCCACGC TGCATACATC CACCAACAGA TTTGTCAGTG 
GTGATCTCCA TCGGAGTTAT TGGTGTAGTC GGAGTCATAA CCTTATTCAT GTTATTTGTC 
TGTTTCTTCC TGAGGCGTCC TGGTAAAATG CCCGCTGTTC TGAAATTGGC TGTGAATGGC 
TGGCTCCCAA TGAATGTAGG ACAAACCGAG GTGGAATCGG TCACTCAGAA GGGATGGCTA 
CTGAACAGCA ACAAAATCGC AGAAAAGACC AAAGCATTCG ATGAAATAGA GGAGCTTTCT 
GAGGATGAAA AAGAGAGAAG GGAAAGCACT GACAGCGGCG TGAGCATCGG CCAGCAGGAC 
TCGATCAAAA ACAGACCCCA AAGAGAGGAG GACAGCGGCT GTGGAAGTCT CACAGGAACC 
GAGGACAGCC TTAGCAGTGG AAGAAGAAGC CTTGAGGAGC TTCCTTTCCT GGATGGAGGA 
GGAAACAGCA GTAGTGTTGA AGGAACAAGA GAGGACAGTG GATTGGGAAT TCAAACCCAA 
GATATTTCTG ACAAACCCAA GCCTATGCAT GATGATCTCT TATCTGAGAT TGTAGTAATT 
GGGGATGGGT ATCGTAGTCA GAGTCCTTCA GCTGAGGCTG AAACCGAAAC AACCATTCAA 
TGTGATGAAG ACGCTAATAT GGTTAGTCGC ACCAATGGCT ATCGATCTGG TCAAGTCACA 
TGCTTGTGTT CTGACTCCGA AACTTGCATG TGGTGCAAAA CCAGGAAGCA CCTTACAGAC 
TGTGATTCAT TTTCTCACAA ACAAACTGTT AATGACAATG ATAGATCAAG CTATTTGAAA 
AAATCTCCTT TAGAGACTGT GAATATGTTT GGACTGGATG ATCTATCCTG CCATTCAGAC 
AAAACAGAGG AGTCGTCACT TTTCATTACA TGTCCACTAC TTTTAAAAGA GCCTTACAAG 
CTGGACACTT TGCCTCTTAC ATTAGGAGAC GTAGAGCTGA CATTTACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071093.1
CDS	129..1739
Translation	MDWTLWICFLVCCTPGLIAGEQKKLNLTFDIWEGNVTVLWDPPNKAPVPAFYQVKLALDTGPHAKWNIVQECDMITETKCNLGNFIFKPTMKVKIELISGNNESSWLVIRRIRLDYIKLLSPDFDLFSGPNTVKVKIHRKPFLKELFPYGLSYSAILYPRGQESKAKTLSDDDDEDGEVEFTDLSYWQTYCVRVRLEITSIAVSNTSLPRCIHPPTDLSVVISIGVIGVVGVITLFMLFVCFFLRRPGKMPAVLKLAVNGWLPMNVGQTEVESVTQKGWLLNSNKIAEKTKAFDEIEELSEDEKERRESTDSGVSIGQQDSIKNRPQREEDSGCGSLTGTEDSLSSGRRSLEELPFLDGGGNSSSVEGTREDSGLGIQTQDISDKPKPMHDDLLSEIVVIGDGYRSQSPSAEAETETTIQCDEDANMVSRTNGYRSGQVTCLCSDSETCMWCKTRKHLTDCDSFSHKQTVNDNDRSSYLKKSPLETVNMFGLDDLSCHSDKTEESSLFITCPLLLKEPYKLDTLPLTLGDVELTFT

Target ORF information:

RefSeq Version	NM_001077625.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interleukin 10 receptor, alpha (il10ra), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077625.1

ORF Insert Sequence:

ATGGATTGGA CCTTGTGGAT TTGTTTTCTA GTCTGCTGTA CCCCAGGATT GATAGCAGGT 
GAGCAGAAGA AATTAAATCT GACGTTCGAC ATATGGGAGG GAAATGTAAC TGTTCTGTGG 
GATCCCCCCA ATAAAGCCCC CGTACCTGCC TTTTATCAAG TAAAACTAGC ACTAGACACT 
GGTCCTCATG CTAAATGGAA CATTGTGCAA GAATGTGACA TGATTACAGA AACTAAATGT 
AATCTTGGAA ACTTTATATT CAAACCTACA ATGAAAGTAA AAATTGAACT GATAAGTGGA 
AATAACGAAT CTTCGTGGTT GGTGATAAGA AGGATTCGTT TAGATTATAT TAAGCTGTTG 
TCTCCAGACT TTGACCTGTT CTCAGGGCCA AATACGGTGA AGGTTAAGAT TCACAGAAAG 
CCATTTCTCA AAGAGCTTTT TCCATATGGA CTAAGTTACT CTGCCATTCT GTATCCGAGA 
GGACAGGAGA GTAAGGCTAA AACATTGAGT GATGATGATG ATGAAGATGG TGAAGTGGAG 
TTTACTGATT TATCATATTG GCAAACGTAT TGTGTTCGTG TGAGATTGGA GATCACATCT 
ATTGCAGTGA GCAACACCTC ATTGCCACGC TGCATACATC CACCAACAGA TTTGTCAGTG 
GTGATCTCCA TCGGAGTTAT TGGTGTAGTC GGAGTCATAA CCTTATTCAT GTTATTTGTC 
TGTTTCTTCC TGAGGCGTCC TGGTAAAATG CCCGCTGTTC TGAAATTGGC TGTGAATGGC 
TGGCTCCCAA TGAATGTAGG ACAAACCGAG GTGGAATCGG TCACTCAGAA GGGATGGCTA 
CTGAACAGCA ACAAAATCGC AGAAAAGACC AAAGCATTCG ATGAAATAGA GGAGCTTTCT 
GAGGATGAAA AAGAGAGAAG GGAAAGCACT GACAGCGGCG TGAGCATCGG CCAGCAGGAC 
TCGATCAAAA ACAGACCCCA AAGAGAGGAG GACAGCGGCT GTGGAAGTCT CACAGGAACC 
GAGGACAGCC TTAGCAGTGG AAGAAGAAGC CTTGAGGAGC TTCCTTTCCT GGATGGAGGA 
GGAAACAGCA GTAGTGTTGA AGGAACAAGA GAGGACAGTG GATTGGGAAT TCAAACCCAA 
GATATTTCTG ACAAACCCAA GCCTATGCAT GATGATCTCT TATCTGAGAT TGTAGTAATT 
GGGGATGGGT ATCGTAGTCA GAGTCCTTCA GCTGAGGCTG AAACCGAAAC AACCATTCAA 
TGTGATGAAG ACGCTAATAT GGTTAGTCGC ACCAATGGCT ATCGATCTGG TCAAGTCACA 
TGCTTGTGTT CTGACTCCGA AACTTGCATG TGGTGCAAAA CCAGGAAGCA CCTTACAGAC 
TGTGATTCAT TTTCTCACAA ACAAACTGTT AATGACAATG ATAGATCAAG CTATTTGAAA 
AAATCTCCTT TAGAGACTGT GAATATGTTT GGACTGGATG ATCTATCCTG CCATTCAGAC 
AAAACAGAGG AGTCGTCACT TTTCATTACA TGTCCACTAC TTTTAAAAGA GCCTTACAAG 
CTGGACACTT TGCCTCTTAC ATTAGGAGAC GTAGAGCTGA CATTTACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polystyrene microplastics induce microbiota dysbiosis and inflammation in the gut of adult zebrafish.
Environmental pollution (Barking, Essex : 1987)235322-329(2018 Jan)
Jin Y,Xia J,Pan Z,Yang J,Wang W,Fu Z

Cloning, identification of the two cytokine receptor family B subunits CRFB1 and CRFB5 from grass carp (Ctenopharyngodon idella).
Fish & shellfish immunology45(2)211-20(2015 Aug)
Chen H,Liu W,Wang B,Mao H,Sun Z,Hou Q,Mi Y,Fan L,Hu C

Koi herpesvirus encodes and expresses a functional interleukin-10.
Journal of virology86(21)11512-20(2012 Nov)
Sunarto A,Liongue C,McColl KA,Adams MM,Bulach D,Crane MS,Schat KA,Slobedman B,Barnes AC,Ward AC,Walker PJ

Identification and molecular characterization of the interleukin-10 receptor 1 of the zebrafish (Danio rerio) and the goldfish (Carassius auratus L.).
Developmental and comparative immunology36(2)408-17(2012 Feb)
Grayfer L,Belosevic M