tsen2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tsen2
Entrez Gene ID	767713
Full Name	TSEN2 tRNA splicing endonuclease subunit
Synonyms	zgc:154067
General protein information	Preferred Names TSEN2 tRNA splicing endonuclease subunit Names tRNA-splicing endonuclease subunit Sen2 tRNA splicing endonuclease 2 homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001076651.1	NP_001070119.1	tRNA-splicing endonuclease subunit Sen2

Pathways from BioSystems

Homologies

	TSEN2	XP_001086382.2
Mus musculus (house mouse)	Tsen2	NP_950198.1
Danio rerio (zebrafish)	tsen2	NP_001070119.1
Pan troglodytes (chimpanzee)	TSEN2	XP_003950080.1
Gallus gallus (chicken)	TSEN2	NP_001025765.1
Homo sapiens (human)	TSEN2	NP_001138864.1
Canis lupus familiaris (dog)	TSEN2	XP_541765.1
Bos taurus (cattle)	TSEN2	NP_001035942.1
Rattus norvegicus (Norway rat)	Tsen2	NP_001014079.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Differential expression of synapsin genes during early zebrafish development.
Garbarino G, Costa S, Pestarino M, Candiani S
Neuroscience280351-67(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tsen2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tsen2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03213	NM_001076651.1 Latest version!	Danio rerio TSEN2 tRNA splicing endonuclease subunit (tsen2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tsen2 ( NM_001076651.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070119.1 Danio rerio TSEN2 tRNA splicing endonuclease subunit (tsen2), mRNA.

Price & Availability↑

CloneID	ODa03213
Clone ID Related Accession (Same CDS sequence)	NM_001076651.1
Accession Version	NM_001076651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	tRNA-splicing endonuclease subunit Sen2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124530.1. ##Evidence-Data-START## Transcript exon combination :: BC124530.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA4476740, SAMEA898401 [ECO:0000350] ##Evidence-Data-END##

ATGACGGACG CTGTGTTTCA GGCGCCTAAG CGGCGGGCGA GGGTTTATGA GTGTTTTGAA 
GCGGCTCTTC CTGTCCCGCT GAACAGCGCA GATGATGGAG ATCATACACT CCTATGCAGA 
GCAGAGATTA TCGGTCATCA TGTGTCCGTC ACAGACCCTG CTCATATACA ACAGCTGTAC 
CACAGGGGTT ACTTTGGTAA AGGGGTTTTC TCCAGATCCA GACCTGAGCA TATGATCTCT 
CCACAGTGGA AATATGTTGG TGACAGATGT CTACCTGTGA TTTCCTTATC AGAGTATCAG 
AAGCGGCTCA GCTTGGCTCG TGCAGCTCTG GTGATGCAGG ATCTGGAGGA GGAATCAGTC 
AATAAAGCAC TGCAGATCCT CACCGAACCC GTGGAGTTTA ACACTGAAGA ACAGCAAGAG 
CCAATATCAA AATCCAACTG TGATGAGTCA CTAATGGACC AAACCATGGA CACTGATTTA 
ATCCCGGATC AGACTGAGTC TGCAAAGCCT CAGCGGCAGG GAAACCCACT GTATGATCCT 
CTGGCTGAAA TATATCCCGA GAAACTGGAC CAGAGTTTAA AAGCCGATGA GAAATGCAGA 
GATCACAATG ACTGGATCTC TCAGTGTGGC TGCAGAGCGA ATGAGGAGCG AATGAACAGA 
ACCATGCACA AATCTCAAAC CTATGAATAT GTCCTGGTGG AGGAGTCTGA AGATGAGCGC 
AGTCAGCACA TTACCCAGGA GAACAGCTCT ACTGATCAGC CGGCTGAGCT GGTGTGCAGA 
ATCAACCCGT TCCCCATGCT GGAGTACCTG CAGCTCAGTT ATGAAGAGGC TTTTTTTCTG 
GTCTATGCTC TTGGATGCTT GTCCATTTAT TATAATGGGG AGGCTCTGTC TGTCGCTCAG 
CTGTGGACGA TGTTTCGCTC GCTGCAGCCA AACTTCTGCT TCTCCTACGC GGCGTATCAT 
TATTACCGCA GTAAAGGCTG GGTGCCGAAG TCTGGAGTTA AATACGGCAC CGACTTAATG 
CTCTACAGAA AAGGACCGCC GTTTTATCAT GCAAGTTATT CAGTGGTTGT GGATACAGTA 
GACGCGTCTT TCAGAAGATC ATCTCTGCGT CCCTTCAGCT GGAGATCTCT GGCCACTCTC 
AGCAGAACCA CTGGAAACGT CTCCAAGGAA CTGATGCTCT GCTTCATCAT CACTCCATCA 
GATGTGACTG GAGATCTGCT GGTTTCTCCT CAGTGTCTCA AACGCTTCTC AGTGCAGGAG 
GTGCTGATGA GCAGATGGGT TTCCTCCAAA GAGCGAACCG AGCAGGAGGA AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070119.1
CDS	124..1440
Translation	MTDAVFQAPKRRARVYECFEAALPVPLNSADDGDHTLLCRAEIIGHHVSVTDPAHIQQLYHRGYFGKGVFSRSRPEHMISPQWKYVGDRCLPVISLSEYQKRLSLARAALVMQDLEEESVNKALQILTEPVEFNTEEQQEPISKSNCDESLMDQTMDTDLIPDQTESAKPQRQGNPLYDPLAEIYPEKLDQSLKADEKCRDHNDWISQCGCRANEERMNRTMHKSQTYEYVLVEESEDERSQHITQENSSTDQPAELVCRINPFPMLEYLQLSYEEAFFLVYALGCLSIYYNGEALSVAQLWTMFRSLQPNFCFSYAAYHYYRSKGWVPKSGVKYGTDLMLYRKGPPFYHASYSVVVDTVDASFRRSSLRPFSWRSLATLSRTTGNVSKELMLCFIITPSDVTGDLLVSPQCLKRFSVQEVLMSRWVSSKERTEQEEI

Target ORF information:

RefSeq Version	NM_001076651.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TSEN2 tRNA splicing endonuclease subunit (tsen2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076651.1

ORF Insert Sequence:

ATGACGGACG CTGTGTTTCA GGCGCCTAAG CGGCGGGCGA GGGTTTATGA GTGTTTTGAA 
GCGGCTCTTC CTGTCCCGCT GAACAGCGCA GATGATGGAG ATCATACACT CCTATGCAGA 
GCAGAGATTA TCGGTCATCA TGTGTCCGTC ACAGACCCTG CTCATATACA ACAGCTGTAC 
CACAGGGGTT ACTTTGGTAA AGGGGTTTTC TCCAGATCCA GACCTGAGCA TATGATCTCT 
CCACAGTGGA AATATGTTGG TGACAGATGT CTACCTGTGA TTTCCTTATC AGAGTATCAG 
AAGCGGCTCA GCTTGGCTCG TGCAGCTCTG GTGATGCAGG ATCTGGAGGA GGAATCAGTC 
AATAAAGCAC TGCAGATCCT CACCGAACCC GTGGAGTTTA ACACTGAAGA ACAGCAAGAG 
CCAATATCAA AATCCAACTG TGATGAGTCA CTAATGGACC AAACCATGGA CACTGATTTA 
ATCCCGGATC AGACTGAGTC TGCAAAGCCT CAGCGGCAGG GAAACCCACT GTATGATCCT 
CTGGCTGAAA TATATCCCGA GAAACTGGAC CAGAGTTTAA AAGCCGATGA GAAATGCAGA 
GATCACAATG ACTGGATCTC TCAGTGTGGC TGCAGAGCGA ATGAGGAGCG AATGAACAGA 
ACCATGCACA AATCTCAAAC CTATGAATAT GTCCTGGTGG AGGAGTCTGA AGATGAGCGC 
AGTCAGCACA TTACCCAGGA GAACAGCTCT ACTGATCAGC CGGCTGAGCT GGTGTGCAGA 
ATCAACCCGT TCCCCATGCT GGAGTACCTG CAGCTCAGTT ATGAAGAGGC TTTTTTTCTG 
GTCTATGCTC TTGGATGCTT GTCCATTTAT TATAATGGGG AGGCTCTGTC TGTCGCTCAG 
CTGTGGACGA TGTTTCGCTC GCTGCAGCCA AACTTCTGCT TCTCCTACGC GGCGTATCAT 
TATTACCGCA GTAAAGGCTG GGTGCCGAAG TCTGGAGTTA AATACGGCAC CGACTTAATG 
CTCTACAGAA AAGGACCGCC GTTTTATCAT GCAAGTTATT CAGTGGTTGT GGATACAGTA 
GACGCGTCTT TCAGAAGATC ATCTCTGCGT CCCTTCAGCT GGAGATCTCT GGCCACTCTC 
AGCAGAACCA CTGGAAACGT CTCCAAGGAA CTGATGCTCT GCTTCATCAT CACTCCATCA 
GATGTGACTG GAGATCTGCT GGTTTCTCCT CAGTGTCTCA AACGCTTCTC AGTGCAGGAG 
GTGCTGATGA GCAGATGGGT TTCCTCCAAA GAGCGAACCG AGCAGGAGGA AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Differential expression of synapsin genes during early zebrafish development.
Neuroscience280351-67(2014 Nov)
Garbarino G,Costa S,Pestarino M,Candiani S