fgf16 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fgf16
Entrez Gene ID	692065
Full Name	fibroblast growth factor 16
General protein information	Preferred Names fibroblast growth factor 16 Names fibroblast growth factor 16 fibroblast growh factor 16
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040407.1	NP_001035497.1	fibroblast growth factor 16

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FGF16	NP_001179706.1
	FGF16	XP_001098950.1
Canis lupus familiaris (dog)	FGF16	XP_549094.2
Mus musculus (house mouse)	Fgf16	NP_085117.2
Danio rerio (zebrafish)	fgf16	NP_001035497.1
Xenopus tropicalis (tropical clawed frog)	fgf16	XP_002931859.1
Homo sapiens (human)	FGF16	NP_003859.1
Pan troglodytes (chimpanzee)	FGF16	XP_529049.2
Rattus norvegicus (Norway rat)	Fgf16	NP_068639.1
Gallus gallus (chicken)	FGF16	NP_001038115.1

Gene Ontology
Bibliography

Related articles in PubMed

Dissection of zebrafish
Sugimoto K, Hui SP, Sheng DZ, Kikuchi K
eLife6(2017 May)

Fgf signalling controls diverse aspects of fin regeneration.
Shibata E, Yokota Y, Horita N, Kudo A, Abe G, Kawakami K, Kawakami A
Development (Cambridge, England)143(16)2920-9(2016 Aug)

Nipbl and mediator cooperatively regulate gene expression to control limb development.
Muto A, Ikeda S, Lopez-Burks ME, Kikuchi Y, Calof AL, Lander AD, Schilling TF
PLoS genetics10(9)e1004671(2014 Sep)

Identification of three novel FGF16 mutations in X-linked recessive fusion of the fourth and fifth metacarpals and possible correlation with heart disease.
Laurell T, Nilsson D, Hofmeister W, Lindstrand A, Ahituv N, Vandermeer J, Amilon A, Anner?n G, Arner M, Pettersson M, J?ntti N, Rosberg HE, Cattini PA, Nordenskj?ld A, M?kitie O, Grigelioniene G, Nordgren A
Molecular genetics & genomic medicine2(5)402-11(2014 Sep)

Fgf16 is required for specification of GABAergic neurons and oligodendrocytes in the zebrafish forebrain.
Miyake A, Chitose T, Kamei E, Murakami A, Nakayama Y, Konishi M, Itoh N
PloS one9(10)e110836(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of the present study indicate that Fgf16 signaling, which is regulated by the downstream pathways of Hh-Fgf19 in the forebrain, is involved in forebrain development.
Title: Fgf16 is required for specification of GABAergic neurons and oligodendrocytes in the zebrafish forebrain.

These findings have revealed that Fgf16, a newly identified AER factor, plays a crucial role in pectoral fin bud outgrowth by mediating the interactions of AER-mesenchyme and AER-ZPA.
Title: Fgf16 is essential for pectoral fin bud formation in zebrafish.

The following fgf16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fgf16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31078	NM_001040407.1 Latest version!	Danio rerio fibroblast growth factor 16 (fgf16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fgf16 ( NM_001040407.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001035497.1 Danio rerio fibroblast growth factor 16 (fgf16), mRNA.

Price & Availability↑

CloneID	ODa31078
Clone ID Related Accession (Same CDS sequence)	NM_001040407.1
Accession Version	NM_001040407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Danio rerio(zebrafish)
Product	fibroblast growth factor 16
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB201764.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB201764.1, BC163741.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAGG TGGCTGGATT TCTCGGGAGC ATCGATCTGG ATTTGCAGGC TTTTCCAGCA 
TTGGGGAACG TGCCTCTGTC AGATGGTTTG AATGAAAGAT TGGGTCTGAT TGAGGGCAGA 
CTGCAGCGCG GATCGCTCAC GGATTTTGGC CACCTGAAAG GCATCCTGCG CAGGAGACAG 
CTCTACTGCC GCACCGGCTT CCAGCTGGAG ATATTTCCCA ACGGGACGGT GCACGGGACT 
AGACAGGACC ACAGCAGATT CGGTATTCTG GAGTTTATCA GTCTGGCCGT CGGGTTGGTG 
AGCATACGAG GCGTGGATGC TGGTCTCTAT CTGGGGATGA ATGAAAAAGG TGAACTCTAT 
GGGTCGAAGA AGCTGACGGC TGAGTGTGTC TTCCGTGAGC AGTTTGAAGA GAACTGGTAC 
AACACGTATG CGTCGACACT CTTCAAGCAT GCAGACACTG GGAGGTACTA TTACGTGGCT 
CTAAACAAGG ACGGCTCGCC TCGAGAAGGC AGCAGGACTA AAAAGCATCA GAAGTTGACT 
CACTTCCTGC CCAGGCCCGT CGAGATAGAA AAGATACCCC AAGCTTACAG AGAATTGTTC 
CAGCATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035497.1
CDS	1..612
Translation	MAEVAGFLGSIDLDLQAFPALGNVPLSDGLNERLGLIEGRLQRGSLTDFGHLKGILRRRQLYCRTGFQLEIFPNGTVHGTRQDHSRFGILEFISLAVGLVSIRGVDAGLYLGMNEKGELYGSKKLTAECVFREQFEENWYNTYASTLFKHADTGRYYYVALNKDGSPREGSRTKKHQKLTHFLPRPVEIEKIPQAYRELFQHR

Target ORF information:

RefSeq Version	NM_001040407.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fibroblast growth factor 16 (fgf16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040407.1

ORF Insert Sequence:

ATGGCAGAGG TGGCTGGATT TCTCGGGAGC ATCGATCTGG ATTTGCAGGC TTTTCCAGCA 
TTGGGGAACG TGCCTCTGTC AGATGGTTTG AATGAAAGAT TGGGTCTGAT TGAGGGCAGA 
CTGCAGCGCG GATCGCTCAC GGATTTTGGC CACCTGAAAG GCATCCTGCG CAGGAGACAG 
CTCTACTGCC GCACCGGCTT CCAGCTGGAG ATATTTCCCA ACGGGACGGT GCACGGGACT 
AGACAGGACC ACAGCAGATT CGGTATTCTG GAGTTTATCA GTCTGGCCGT CGGGTTGGTG 
AGCATACGAG GCGTGGATGC TGGTCTCTAT CTGGGGATGA ATGAAAAAGG TGAACTCTAT 
GGGTCGAAGA AGCTGACGGC TGAGTGTGTC TTCCGTGAGC AGTTTGAAGA GAACTGGTAC 
AACACGTATG CGTCGACACT CTTCAAGCAT GCAGACACTG GGAGGTACTA TTACGTGGCT 
CTAAACAAGG ACGGCTCGCC TCGAGAAGGC AGCAGGACTA AAAAGCATCA GAAGTTGACT 
CACTTCCTGC CCAGGCCCGT CGAGATAGAA AAGATACCCC AAGCTTACAG AGAATTGTTC 
CAGCATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissection of zebrafish
eLife6(2017 May)
Sugimoto K,Hui SP,Sheng DZ,Kikuchi K

Fgf signalling controls diverse aspects of fin regeneration.
Development (Cambridge, England)143(16)2920-9(2016 Aug)
Shibata E,Yokota Y,Horita N,Kudo A,Abe G,Kawakami K,Kawakami A

Nipbl and mediator cooperatively regulate gene expression to control limb development.
PLoS genetics10(9)e1004671(2014 Sep)
Muto A,Ikeda S,Lopez-Burks ME,Kikuchi Y,Calof AL,Lander AD,Schilling TF

Identification of three novel FGF16 mutations in X-linked recessive fusion of the fourth and fifth metacarpals and possible correlation with heart disease.
Molecular genetics & genomic medicine2(5)402-11(2014 Sep)
Laurell T,Nilsson D,Hofmeister W,Lindstrand A,Ahituv N,Vandermeer J,Amilon A,Anner?n G,Arner M,Pettersson M,J?ntti N,Rosberg HE,Cattini PA,Nordenskj?ld A,M?kitie O,Grigelioniene G,Nordgren A

Fgf16 is required for specification of GABAergic neurons and oligodendrocytes in the zebrafish forebrain.
PloS one9(10)e110836(2014)
Miyake A,Chitose T,Kamei E,Murakami A,Nakayama Y,Konishi M,Itoh N