retsatl cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	retsatl
Entrez Gene ID	677660
Full Name	retinol saturase (all-trans-retinol 13,14-reductase) like
Synonyms	wu:fi35b01,zgc:123334
General protein information	Preferred Names retinol saturase (all-trans-retinol 13,14-reductase) like Names retinol saturase-like zRetSat B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040004.2	NP_001035093.2	inactive all-trans-retinol 13,14-reductase precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	retsatl	NP_001035093.2
Danio rerio (zebrafish)	LOC563241	XP_003199610.1
Xenopus tropicalis (tropical clawed frog)	LOC100488563	XP_002935591.2
Arabidopsis thaliana (thale cress)	CRTISO	NP_172167.2
Oryza sativa (rice)	Os11g0572700	NP_001068127.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Early depletion of primordial germ cells in zebrafish promotes testis formation.
Tzung KW, Goto R, Saju JM, Sreenivasan R, Saito T, Arai K, Yamaha E, Hossain MS, Calvert ME, Orb?n L
Stem cell reports4(1)61-73(2015 Jan)

The earliest transcribed zygotic genes are short, newly evolved, and different across species.
Heyn P, Kircher M, Dahl A, Kelso J, Tomancak P, Kalinka AT, Neugebauer KM
Cell reports6(2)285-92(2014 Jan)

Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.
Sreenivasan R, Cai M, Bartfai R, Wang X, Christoffels A, Orban L
PloS one3(3)e1791(2008 Mar)

Specificity of zebrafish retinol saturase: formation of all-trans-13,14-dihydroretinol and all-trans-7,8- dihydroretinol.
Moise AR, Isken A, Dom?nguez M, de Lera AR, von Lintig J, Palczewski K
Biochemistry46(7)1811-20(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following retsatl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the retsatl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01896	NM_001040004.2 Latest version!	Danio rerio retinol saturase (all-trans-retinol 13,14-reductase) like (retsatl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

retsatl ( NM_001040004.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001035093.2 Danio rerio retinol saturase (all-trans-retinol 13,14-reductase) like (retsatl), mRNA.

Price & Availability↑

CloneID	ODa01896
Clone ID Related Accession (Same CDS sequence)	NM_001040004.2
Accession Version	NM_001040004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Danio rerio(zebrafish)
Product	inactive all-trans-retinol 13,14-reductase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC107980.1. On Aug 23, 2007 this sequence version replaced NM_001040004.1. Sequence Note: The sequence BC107980.1 is from a chimeric clone. Only the retinol saturase-like containing sequence was propagated into this RefSeq record. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA708832, SAMEA898399 [ECO:0000348] ##Evidence-Data-END##

ATGTGGTGGA TTTTGCTGTT CTTAGAATGG TTTGTTGACT GGGCTCGTGG CACATTCTGG 
TACCTGTTTG GCAGGAGAAG TGGCCTGTGC AAGGGTACCA TCAGTCCTCA GGGCCCACTA 
GTAGTCGACC AGGAGAAAAA AGACAAAGTG CTTCAAAAAG AATATGCTTT AAATGAAGTA 
CCCGATAACT TGGATGTCAT TGTGATTGGT AGTGGCATTG GTGGCCTGAC TGCAGCTGCA 
GTTTTAGCGA GGCTCGGAAA GAAAGTCCTA GTGCTGGAGC AAGACAAGCA GGCAGGAGGC 
CTTTGTAAGA CTTTCACTGA GAAGGGCTTT GAGTTTGACT GTGGCTTCTA TTATGTTGGT 
CAACTTCATG AGAACAGCTT CCTGAAGATT GCTTTGGATT TGATTACTGA TGGCCAAGTG 
CACTTTGCTG AGCAAGGTTC ACATGTGGAA ACTGTTGTTA TTGGTAAAGG GCCTGAGTGT 
AAAGAATACA CTATCTATAA TGGTAAAAAG CAGATGGAGG CTCGTCTGAA GAAACAGTTC 
CCCAATGATG CTAAAGCAGT AGAGGAATTC TTTAAAATAA TGAAGATTTG TTCTGAGAAG 
GTCCGTCTGC TTTGTATGCT GAAGATGGTC CCACTCTGGT TTGCTCGCTT CATATTGAGG 
ACTGGAATTG CTGACTTTAT TTCCCCAATT TTAAAATACT CCCGCACCAG TATATCAGAG 
GTGGTCAAGT CTCTCACCAG CAACCAGGAT CTCCTCACAG TGTTCTCTAA AACATTTTGT 
GGTGTTCCAC CCAAAAACTC CAGCTGCATG ATTGATGCCC TCATACTACA CCACTCAAAG 
CGTGGTGTGT ACTATCCTCA GGGTGGTGCT AGTGAAATCC CGTATCATAT CATCCAGGTT 
TTGGAGAAAC ATGGTGGAAA AGTCCTGGTC AATGCTCCAG TTTCTAGAGT TCTAGTTGAT 
GAGCAACAAA ATGCATACGG TGTGGCTGTG AAGACTGGAG ATGAAGATAT TGAAATCAAG 
GCTCCAGTGG TTGTTTCAAA TGCTGGTGTG TTCACTACTT TCCAGAAGCT CCTGACTCCA 
GAGATCCAGG CAGATCCCCA GGTCCAGGAA TATCTTAAAG CTCTGAAACC AGGCAAAGGC 
TTTTTTCAAG TATTTGCTGG CTTCAATGCC ACAATGGAAG AACTAGGCAT CTCTTCAACT 
GACATGCGGC TCTATAAGGG CAACAACGTT GATGAGATGA TGGAGGAGTA CTTTGCTTCA 
GACAAGCAAG ATGCACCTGA TAATGTCCCC ATGATGTATC TTTCCTTCCC CTCTGCTAAG 
GATCCCACCT CAATCACTCG TTTCCCAGGG CAATCACGTA TGGTGATCCA TACTTTGGTC 
AATCCAAAGT GGTTTGAGCA GTGGGAAAAC GTTAATGAGG CTGAAAGAGG TGAAGAATAT 
GAGAACTACA AGATGAGATT TGCAAATCAT CTGTTTGACT GGGCTTGTGT CCGTTTTCCC 
CAGTCGAAAG AAAAGGTTGC ATTGCTGCAT GCTGTCACTC CCATCAATAT GCATGGCCTG 
GGAGCCTCCT ATTGTTCCAT GTCTGCGGAG CACAATCTGG AGAGATACCA GCCTCTGAAC 
ATTGCCACCA TCAGGTGCAA TACACCAGTC AAGAACCTCT ACCTGTCAGG ACAAGATATC 
TTCACTGCTG GTTATTCTGG AGCTCTGCAT GGTGGCTTTC TCTGTGCTTC AACTGTTATG 
GATCGCTGTC TGCATATTGA CCTTCTGCTC CAACAAAAGA AGCTCAAGAG CAAGTCTGTC 
AAGAAACTAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035093.2
CDS	125..1939
Translation	MWWILLFLEWFVDWARGTFWYLFGRRSGLCKGTISPQGPLVVDQEKKDKVLQKEYALNEVPDNLDVIVIGSGIGGLTAAAVLARLGKKVLVLEQDKQAGGLCKTFTEKGFEFDCGFYYVGQLHENSFLKIALDLITDGQVHFAEQGSHVETVVIGKGPECKEYTIYNGKKQMEARLKKQFPNDAKAVEEFFKIMKICSEKVRLLCMLKMVPLWFARFILRTGIADFISPILKYSRTSISEVVKSLTSNQDLLTVFSKTFCGVPPKNSSCMIDALILHHSKRGVYYPQGGASEIPYHIIQVLEKHGGKVLVNAPVSRVLVDEQQNAYGVAVKTGDEDIEIKAPVVVSNAGVFTTFQKLLTPEIQADPQVQEYLKALKPGKGFFQVFAGFNATMEELGISSTDMRLYKGNNVDEMMEEYFASDKQDAPDNVPMMYLSFPSAKDPTSITRFPGQSRMVIHTLVNPKWFEQWENVNEAERGEEYENYKMRFANHLFDWACVRFPQSKEKVALLHAVTPINMHGLGASYCSMSAEHNLERYQPLNIATIRCNTPVKNLYLSGQDIFTAGYSGALHGGFLCASTVMDRCLHIDLLLQQKKLKSKSVKKLE

Target ORF information:

RefSeq Version	NM_001040004.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio retinol saturase (all-trans-retinol 13,14-reductase) like (retsatl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040004.2

ORF Insert Sequence:

ATGTGGTGGA TTTTGCTGTT CTTAGAATGG TTTGTTGACT GGGCTCGTGG CACATTCTGG 
TACCTGTTTG GCAGGAGAAG TGGCCTGTGC AAGGGTACCA TCAGTCCTCA GGGCCCACTA 
GTAGTCGACC AGGAGAAAAA AGACAAAGTG CTTCAAAAAG AATATGCTTT AAATGAAGTA 
CCCGATAACT TGGATGTCAT TGTGATTGGT AGTGGCATTG GTGGCCTGAC TGCAGCTGCA 
GTTTTAGCGA GGCTCGGAAA GAAAGTCCTA GTGCTGGAGC AAGACAAGCA GGCAGGAGGC 
CTTTGTAAGA CTTTCACTGA GAAGGGCTTT GAGTTTGACT GTGGCTTCTA TTATGTTGGT 
CAACTTCATG AGAACAGCTT CCTGAAGATT GCTTTGGATT TGATTACTGA TGGCCAAGTG 
CACTTTGCTG AGCAAGGTTC ACATGTGGAA ACTGTTGTTA TTGGTAAAGG GCCTGAGTGT 
AAAGAATACA CTATCTATAA TGGTAAAAAG CAGATGGAGG CTCGTCTGAA GAAACAGTTC 
CCCAATGATG CTAAAGCAGT AGAGGAATTC TTTAAAATAA TGAAGATTTG TTCTGAGAAG 
GTCCGTCTGC TTTGTATGCT GAAGATGGTC CCACTCTGGT TTGCTCGCTT CATATTGAGG 
ACTGGAATTG CTGACTTTAT TTCCCCAATT TTAAAATACT CCCGCACCAG TATATCAGAG 
GTGGTCAAGT CTCTCACCAG CAACCAGGAT CTCCTCACAG TGTTCTCTAA AACATTTTGT 
GGTGTTCCAC CCAAAAACTC CAGCTGCATG ATTGATGCCC TCATACTACA CCACTCAAAG 
CGTGGTGTGT ACTATCCTCA GGGTGGTGCT AGTGAAATCC CGTATCATAT CATCCAGGTT 
TTGGAGAAAC ATGGTGGAAA AGTCCTGGTC AATGCTCCAG TTTCTAGAGT TCTAGTTGAT 
GAGCAACAAA ATGCATACGG TGTGGCTGTG AAGACTGGAG ATGAAGATAT TGAAATCAAG 
GCTCCAGTGG TTGTTTCAAA TGCTGGTGTG TTCACTACTT TCCAGAAGCT CCTGACTCCA 
GAGATCCAGG CAGATCCCCA GGTCCAGGAA TATCTTAAAG CTCTGAAACC AGGCAAAGGC 
TTTTTTCAAG TATTTGCTGG CTTCAATGCC ACAATGGAAG AACTAGGCAT CTCTTCAACT 
GACATGCGGC TCTATAAGGG CAACAACGTT GATGAGATGA TGGAGGAGTA CTTTGCTTCA 
GACAAGCAAG ATGCACCTGA TAATGTCCCC ATGATGTATC TTTCCTTCCC CTCTGCTAAG 
GATCCCACCT CAATCACTCG TTTCCCAGGG CAATCACGTA TGGTGATCCA TACTTTGGTC 
AATCCAAAGT GGTTTGAGCA GTGGGAAAAC GTTAATGAGG CTGAAAGAGG TGAAGAATAT 
GAGAACTACA AGATGAGATT TGCAAATCAT CTGTTTGACT GGGCTTGTGT CCGTTTTCCC 
CAGTCGAAAG AAAAGGTTGC ATTGCTGCAT GCTGTCACTC CCATCAATAT GCATGGCCTG 
GGAGCCTCCT ATTGTTCCAT GTCTGCGGAG CACAATCTGG AGAGATACCA GCCTCTGAAC 
ATTGCCACCA TCAGGTGCAA TACACCAGTC AAGAACCTCT ACCTGTCAGG ACAAGATATC 
TTCACTGCTG GTTATTCTGG AGCTCTGCAT GGTGGCTTTC TCTGTGCTTC AACTGTTATG 
GATCGCTGTC TGCATATTGA CCTTCTGCTC CAACAAAAGA AGCTCAAGAG CAAGTCTGTC 
AAGAAACTAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Early depletion of primordial germ cells in zebrafish promotes testis formation.
Stem cell reports4(1)61-73(2015 Jan)
Tzung KW,Goto R,Saju JM,Sreenivasan R,Saito T,Arai K,Yamaha E,Hossain MS,Calvert ME,Orb?n L

The earliest transcribed zygotic genes are short, newly evolved, and different across species.
Cell reports6(2)285-92(2014 Jan)
Heyn P,Kircher M,Dahl A,Kelso J,Tomancak P,Kalinka AT,Neugebauer KM

Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.
PloS one3(3)e1791(2008 Mar)
Sreenivasan R,Cai M,Bartfai R,Wang X,Christoffels A,Orban L

Specificity of zebrafish retinol saturase: formation of all-trans-13,14-dihydroretinol and all-trans-7,8- dihydroretinol.
Biochemistry46(7)1811-20(2007 Feb)
Moise AR,Isken A,Dom?nguez M,de Lera AR,von Lintig J,Palczewski K