COL6A1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL6A1
Entrez Gene ID	569755
Full Name	collagen, type VI, alpha 1
General protein information	Preferred Names collagen, type VI, alpha 1 Names collagen alpha-1(VI) chain
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306169.3	XP_009304444.1	collagen alpha-1(VI) chain
XM_009306169.2	XP_009304444.1	collagen alpha-1(VI) chain

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col6a1	XP_215375.5
Xenopus tropicalis (tropical clawed frog)	col6a1	XP_002932077.2
Canis lupus familiaris (dog)	COL6A1	XP_003434049.2
Mus musculus (house mouse)	Col6a1	NP_034063.1
Gallus gallus (chicken)	COL6A1	NP_990438.1
Danio rerio (zebrafish)	col6a1	XP_698253.3
Homo sapiens (human)	COL6A1	NP_001839.2
Pan troglodytes (chimpanzee)	COL6A1	XP_001158445.1
	LOC721855	XP_001118050.2
Bos taurus (cattle)	COL6A1	NP_001137337.1

Gene Ontology

The following COL6A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa45843	XM_009306169.3 Latest version!	Danio rerio collagen, type VI, alpha 1 (col6a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa45843	XM_009306169.2	Danio rerio collagen, type VI, alpha 1 (col6a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL6A1 ( XM_009306169.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304444.1 Danio rerio collagen, type VI, alpha 1 (col6a1), mRNA.

Price & Availability↑

CloneID	ODa45843
Clone ID Related Accession (Same CDS sequence)	XM_009306169.3 , XM_009306169.2
Accession Version	XM_009306169.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(VI) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTA ATGGATATAT ATTGACCGCT CTGTGCGCTC TTTGGGCAGG GATCTTCGCT 
CAGGATGTCT CCAGATCCTC CTTCAGAGAG TGTCCTGTGG ACCTGTTCTT TGTGCTGGAC 
ACGTCTGAAA GTGTGGCTCT CAGAGCCAAA CCTCCAGAGT TTTACATCAA CCAGATTAAG 
ACCTTCACAA AGCTGTTCAT CGATGAGCTC AAAGACCTAC GCCAGCCCTG TGATCGCGAT 
GTGACATGGA ACTCGGGAGC GCTGCACTAC AGTGATGACA CAGAGCTGGT CATGGGATTA 
GTCGACCTCA ACACAAAACG TGCTGACCTT AAAGCTGCCA TCGACAGAAT CAAATACATC 
GGCAAGGGAA CGTACACCGA CTGCGCCATC AAGGAGGGCA TCGCTGAGCT GCTCAGAGCG 
GGTTCGCATT ATCATGAGAA TAAATACATT GTGGTGGTGA CGGATGGTCA TCCTATAACC 
GGATATAAGG AACCATGCGG TGGCATTCAG GAGGCCGCCA ATGAGGCTCG ACAACATGCC 
ATTAAAGTGT TTGCTGTGGC CATCTCACCT GACCAGGAGG ACACCAGACT GTCTGTCATT 
GCCACAGATA TAAACTACAG ACAGAACTTC ACCGCTGCAG ACAACTCCCG CTCCACACAA 
ATGAGCACTA TACGCTCCAT CATCCACATG ATTACAAACG AAACAAAGGA TGTGTGCTGC 
TCCTTTGACT GCATCGCTGA TGGAGGCCCT CAAGGACCCC CTGGAGATCC AGGTCAAAGG 
GGAGAGACCG GGAGACCAGG CATGCCAGGA GAAAAAGGAG ACTTTGGGGC TCAGGGAAAT 
CCTGGTGATC CCGGTCCAGT CGGTTACCAA GGAATGAAGG GAGACCAGGG AATAAAAGGT 
GATAAGGGAG AGAGAGGTCA TAAAGGCTTC AAGGGAGACA AAGGTCATCA TGGTCTCGAT 
GGCACGGATG GACGTAAGGG CGAGCCGGGA TTCCCTGGAC TTCCAGGCTG TAAAGGGTCA 
CCAGGGCCAG ATGGTTTGCA AGGAGAATCA GGGCCTAAAG GAGACCCCGG ACAATATGGA 
CGCAAAGGAG AACGAGGAGA TCCTGGCAGA GATGGTGAGC CAGGAAGACC TGGAAACTAC 
GGACCATCTG GGGCCAAGGG AGACAGGGGT CCTCGTGGGG CTAACGGCGA TAAAGGCGAG 
AGAGGAGATG ATGGACCACC AGGACCAGAT GGCCCAAGAG GAGAAAGAGG CCAAGGTGGT 
GAGAAGGGTG AACAAGGGTC TCGAGGTAAC AGAGGTCCCA GAGGCGAGCC TGGTGAACCC 
GGGCCAAGGG GTGAGCAAGG ACGTGAAGGA TCATCCGGAG CAAATGGAGA GCCGGGTGAG 
CAAGGAAGAG CAGGGCAGCC AGGTTATCGT GGCGATGAGG GTCCTGCTGG GCCAGAGGGA 
GATAAAGGGC CCAGAGGAAT CAAAGGGGCT CCAGGAGACA GAGGCCTGAT GGGAGAGAGG 
GGTGAAGACG GACCTCCAGG AAACGGGACG GAGGGTTGTG CTGGCTTTCA GGGTTATCCT 
GGTCCAAGGG GAGATCCTGG AGCACCTGGT GGAAAAGGAA CCCCAGGACC CAAGGGAGAT 
GATGGAGAGC CGGGTGACAC TGGATTAGAT AATGACAGAC TTGGACCACC TGGGACAAAA 
GGAGCCAAAG GTCACAGAGG ACCTGAAGGA AAACCTGGAC CCCCAGGACC ACCCGGACCT 
CAAGGAGCTG ATGAATGTGA GATTCTGGAT ATCATCATGA AGATGTGCTC TTGCTGTGAA 
TGCACATGTG GACCCTTGGA TATTGCCTTT ATCGTGGACA GCTCTGAGAG TATTGGAGCT 
TCTAACTTTG CCATCTCTAA AGAATTCATC GTGACAATAA TGGACAGACT CAAGTTAAGA 
CAGTTTGGTG CCAATGAGTC TCGTATTGGT GTGGTGCAGT ACAGTGGTGA AAAATCTCAA 
GAGGTGGTAC AGCTTGGTGA CCCCAACATC AAGACCCTGA CCGACCTAAA ACAAGCTGTG 
AAAGACTTGC GCTGGCTGGC TGAGGCCACA TACACTGGAG AAGCTCTTCA GTACTCGCTA 
AGAAACATGC TCAGCAAGCT TATCACGGAG AGGAGTGTGG TCCTTGTTCT TACTGATGGA 
CGCTCAGACA CTATTAGGGA TAAAGTGCCA TTAAACGTGC TGTGCGGCAA AGGATTTAAG 
GTTGGTGGGG TTGGAGTGAC GGATTACGCA GAGAGGAAAC CCAATCCGGA ACAGCTTGAT 
GAGGTTGTGT GTAAAGATGA TCCTAGAAAA GGCTTCTCCT TTGTCCTAAA CAACTTTGGC 
CTACTTCTGG ATGACAACTT CCTGCAAAAC CTGACTGAGA AAATCTGTGA AGATAAGAAG 
TGTCCCACAT ATACTTGTCC AATTTCATTT AATGACAACA CCGATGTCCT CATCATGATG 
GACAGCTCGG CCAGCGTTGG CGCCAAGAAC TTCGAGATGA CCAAGGACTT CTCACGGATG 
CTAGCTAAGC GCTTTTTGTC AGCAGAAAGA GGAAATTTCC AGGTTCGAAT TGGTGTTGGC 
CAATACAGCA ACAATGCCAA CTTGGAGGCC GAATTTTCCA CAAACGCCAC TCAGGTGGTC 
GCTCAAATCG CAGACGCCAA GTTCCAGAAT GCAGGTACAC AGGTAACAAA CGCTCTCAAC 
TTCGCCATCG AGCGCTTCAG AGGCGGTAGG ACGAGGAAGA AGAAGCTGCT GGTGTTTTCT 
GATGGTCGTT CTCAAGGTGT TAACTCCATT CAGATTGAGA AAGCCGTGGA GCAGGTGAGC 
AACGCAGGAA TTGAGCTGTA TGTTCTTGCT GTTGGAAATC AGGTGAATGA AGCGCATTTA 
CGCACCTTAG TGAGCCGAGG CCGGCCTTAC GACAACACCT ACGCCTACCG CCATCTTTTC 
AAAGTACCCG ACTACCGCTC ATTGGTCACT GGGGTATTTT ACCAAACTGT GTCCCGCAAG 
ATTTCCCTGG AGACGAGAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304444.1
CDS	174..3197
Translation	MALNGYILTALCALWAGIFAQDVSRSSFRECPVDLFFVLDTSESVALRAKPPEFYINQIKTFTKLFIDELKDLRQPCDRDVTWNSGALHYSDDTELVMGLVDLNTKRADLKAAIDRIKYIGKGTYTDCAIKEGIAELLRAGSHYHENKYIVVVTDGHPITGYKEPCGGIQEAANEARQHAIKVFAVAISPDQEDTRLSVIATDINYRQNFTAADNSRSTQMSTIRSIIHMITNETKDVCCSFDCIADGGPQGPPGDPGQRGETGRPGMPGEKGDFGAQGNPGDPGPVGYQGMKGDQGIKGDKGERGHKGFKGDKGHHGLDGTDGRKGEPGFPGLPGCKGSPGPDGLQGESGPKGDPGQYGRKGERGDPGRDGEPGRPGNYGPSGAKGDRGPRGANGDKGERGDDGPPGPDGPRGERGQGGEKGEQGSRGNRGPRGEPGEPGPRGEQGREGSSGANGEPGEQGRAGQPGYRGDEGPAGPEGDKGPRGIKGAPGDRGLMGERGEDGPPGNGTEGCAGFQGYPGPRGDPGAPGGKGTPGPKGDDGEPGDTGLDNDRLGPPGTKGAKGHRGPEGKPGPPGPPGPQGADECEILDIIMKMCSCCECTCGPLDIAFIVDSSESIGASNFAISKEFIVTIMDRLKLRQFGANESRIGVVQYSGEKSQEVVQLGDPNIKTLTDLKQAVKDLRWLAEATYTGEALQYSLRNMLSKLITERSVVLVLTDGRSDTIRDKVPLNVLCGKGFKVGGVGVTDYAERKPNPEQLDEVVCKDDPRKGFSFVLNNFGLLLDDNFLQNLTEKICEDKKCPTYTCPISFNDNTDVLIMMDSSASVGAKNFEMTKDFSRMLAKRFLSAERGNFQVRIGVGQYSNNANLEAEFSTNATQVVAQIADAKFQNAGTQVTNALNFAIERFRGGRTRKKKLLVFSDGRSQGVNSIQIEKAVEQVSNAGIELYVLAVGNQVNEAHLRTLVSRGRPYDNTYAYRHLFKVPDYRSLVTGVFYQTVSRKISLETRG

Target ORF information:

RefSeq Version	XM_009306169.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type VI, alpha 1 (col6a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306169.3

ORF Insert Sequence:

ATGGCGCTTA ATGGATATAT ATTGACCGCT CTGTGCGCTC TTTGGGCAGG GATCTTCGCT 
CAGGATGTCT CCAGATCCTC CTTCAGAGAG TGTCCTGTGG ACCTGTTCTT TGTGCTGGAC 
ACGTCTGAAA GTGTGGCTCT CAGAGCCAAA CCTCCAGAGT TTTACATCAA CCAGATTAAG 
ACCTTCACAA AGCTGTTCAT CGATGAGCTC AAAGACCTAC GCCAGCCCTG TGATCGCGAT 
GTGACATGGA ACTCGGGAGC GCTGCACTAC AGTGATGACA CAGAGCTGGT CATGGGATTA 
GTCGACCTCA ACACAAAACG TGCTGACCTT AAAGCTGCCA TCGACAGAAT CAAATACATC 
GGCAAGGGAA CGTACACCGA CTGCGCCATC AAGGAGGGCA TCGCTGAGCT GCTCAGAGCG 
GGTTCGCATT ATCATGAGAA TAAATACATT GTGGTGGTGA CGGATGGTCA TCCTATAACC 
GGATATAAGG AACCATGCGG TGGCATTCAG GAGGCCGCCA ATGAGGCTCG ACAACATGCC 
ATTAAAGTGT TTGCTGTGGC CATCTCACCT GACCAGGAGG ACACCAGACT GTCTGTCATT 
GCCACAGATA TAAACTACAG ACAGAACTTC ACCGCTGCAG ACAACTCCCG CTCCACACAA 
ATGAGCACTA TACGCTCCAT CATCCACATG ATTACAAACG AAACAAAGGA TGTGTGCTGC 
TCCTTTGACT GCATCGCTGA TGGAGGCCCT CAAGGACCCC CTGGAGATCC AGGTCAAAGG 
GGAGAGACCG GGAGACCAGG CATGCCAGGA GAAAAAGGAG ACTTTGGGGC TCAGGGAAAT 
CCTGGTGATC CCGGTCCAGT CGGTTACCAA GGAATGAAGG GAGACCAGGG AATAAAAGGT 
GATAAGGGAG AGAGAGGTCA TAAAGGCTTC AAGGGAGACA AAGGTCATCA TGGTCTCGAT 
GGCACGGATG GACGTAAGGG CGAGCCGGGA TTCCCTGGAC TTCCAGGCTG TAAAGGGTCA 
CCAGGGCCAG ATGGTTTGCA AGGAGAATCA GGGCCTAAAG GAGACCCCGG ACAATATGGA 
CGCAAAGGAG AACGAGGAGA TCCTGGCAGA GATGGTGAGC CAGGAAGACC TGGAAACTAC 
GGACCATCTG GGGCCAAGGG AGACAGGGGT CCTCGTGGGG CTAACGGCGA TAAAGGCGAG 
AGAGGAGATG ATGGACCACC AGGACCAGAT GGCCCAAGAG GAGAAAGAGG CCAAGGTGGT 
GAGAAGGGTG AACAAGGGTC TCGAGGTAAC AGAGGTCCCA GAGGCGAGCC TGGTGAACCC 
GGGCCAAGGG GTGAGCAAGG ACGTGAAGGA TCATCCGGAG CAAATGGAGA GCCGGGTGAG 
CAAGGAAGAG CAGGGCAGCC AGGTTATCGT GGCGATGAGG GTCCTGCTGG GCCAGAGGGA 
GATAAAGGGC CCAGAGGAAT CAAAGGGGCT CCAGGAGACA GAGGCCTGAT GGGAGAGAGG 
GGTGAAGACG GACCTCCAGG AAACGGGACG GAGGGTTGTG CTGGCTTTCA GGGTTATCCT 
GGTCCAAGGG GAGATCCTGG AGCACCTGGT GGAAAAGGAA CCCCAGGACC CAAGGGAGAT 
GATGGAGAGC CGGGTGACAC TGGATTAGAT AATGACAGAC TTGGACCACC TGGGACAAAA 
GGAGCCAAAG GTCACAGAGG ACCTGAAGGA AAACCTGGAC CCCCAGGACC ACCCGGACCT 
CAAGGAGCTG ATGAATGTGA GATTCTGGAT ATCATCATGA AGATGTGCTC TTGCTGTGAA 
TGCACATGTG GACCCTTGGA TATTGCCTTT ATCGTGGACA GCTCTGAGAG TATTGGAGCT 
TCTAACTTTG CCATCTCTAA AGAATTCATC GTGACAATAA TGGACAGACT CAAGTTAAGA 
CAGTTTGGTG CCAATGAGTC TCGTATTGGT GTGGTGCAGT ACAGTGGTGA AAAATCTCAA 
GAGGTGGTAC AGCTTGGTGA CCCCAACATC AAGACCCTGA CCGACCTAAA ACAAGCTGTG 
AAAGACTTGC GCTGGCTGGC TGAGGCCACA TACACTGGAG AAGCTCTTCA GTACTCGCTA 
AGAAACATGC TCAGCAAGCT TATCACGGAG AGGAGTGTGG TCCTTGTTCT TACTGATGGA 
CGCTCAGACA CTATTAGGGA TAAAGTGCCA TTAAACGTGC TGTGCGGCAA AGGATTTAAG 
GTTGGTGGGG TTGGAGTGAC GGATTACGCA GAGAGGAAAC CCAATCCGGA ACAGCTTGAT 
GAGGTTGTGT GTAAAGATGA TCCTAGAAAA GGCTTCTCCT TTGTCCTAAA CAACTTTGGC 
CTACTTCTGG ATGACAACTT CCTGCAAAAC CTGACTGAGA AAATCTGTGA AGATAAGAAG 
TGTCCCACAT ATACTTGTCC AATTTCATTT AATGACAACA CCGATGTCCT CATCATGATG 
GACAGCTCGG CCAGCGTTGG CGCCAAGAAC TTCGAGATGA CCAAGGACTT CTCACGGATG 
CTAGCTAAGC GCTTTTTGTC AGCAGAAAGA GGAAATTTCC AGGTTCGAAT TGGTGTTGGC 
CAATACAGCA ACAATGCCAA CTTGGAGGCC GAATTTTCCA CAAACGCCAC TCAGGTGGTC 
GCTCAAATCG CAGACGCCAA GTTCCAGAAT GCAGGTACAC AGGTAACAAA CGCTCTCAAC 
TTCGCCATCG AGCGCTTCAG AGGCGGTAGG ACGAGGAAGA AGAAGCTGCT GGTGTTTTCT 
GATGGTCGTT CTCAAGGTGT TAACTCCATT CAGATTGAGA AAGCCGTGGA GCAGGTGAGC 
AACGCAGGAA TTGAGCTGTA TGTTCTTGCT GTTGGAAATC AGGTGAATGA AGCGCATTTA 
CGCACCTTAG TGAGCCGAGG CCGGCCTTAC GACAACACCT ACGCCTACCG CCATCTTTTC 
AAAGTACCCG ACTACCGCTC ATTGGTCACT GGGGTATTTT ACCAAACTGT GTCCCGCAAG 
ATTTCCCTGG AGACGAGAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A1 ( XM_009306169.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304444.1 Danio rerio collagen, type VI, alpha 1 (col6a1), mRNA.

Price & Availability↑

CloneID	ODa45843
Clone ID Related Accession (Same CDS sequence)	XM_009306169.3 , XM_009306169.2
Accession Version	XM_009306169.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(VI) chain
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTA ATGGATATAT ATTGACCGCT CTGTGCGCTC TTTGGGCAGG GATCTTCGCT 
CAGGATGTCT CCAGATCCTC CTTCAGAGAG TGTCCTGTGG ACCTGTTCTT TGTGCTGGAC 
ACGTCTGAAA GTGTGGCTCT CAGAGCCAAA CCTCCAGAGT TTTACATCAA CCAGATTAAG 
ACCTTCACAA AGCTGTTCAT CGATGAGCTC AAAGACCTAC GCCAGCCCTG TGATCGCGAT 
GTGACATGGA ACTCGGGAGC GCTGCACTAC AGTGATGACA CAGAGCTGGT CATGGGATTA 
GTCGACCTCA ACACAAAACG TGCTGACCTT AAAGCTGCCA TCGACAGAAT CAAATACATC 
GGCAAGGGAA CGTACACCGA CTGCGCCATC AAGGAGGGCA TCGCTGAGCT GCTCAGAGCG 
GGTTCGCATT ATCATGAGAA TAAATACATT GTGGTGGTGA CGGATGGTCA TCCTATAACC 
GGATATAAGG AACCATGCGG TGGCATTCAG GAGGCCGCCA ATGAGGCTCG ACAACATGCC 
ATTAAAGTGT TTGCTGTGGC CATCTCACCT GACCAGGAGG ACACCAGACT GTCTGTCATT 
GCCACAGATA TAAACTACAG ACAGAACTTC ACCGCTGCAG ACAACTCCCG CTCCACACAA 
ATGAGCACTA TACGCTCCAT CATCCACATG ATTACAAACG AAACAAAGGA TGTGTGCTGC 
TCCTTTGACT GCATCGCTGA TGGAGGCCCT CAAGGACCCC CTGGAGATCC AGGTCAAAGG 
GGAGAGACCG GGAGACCAGG CATGCCAGGA GAAAAAGGAG ACTTTGGGGC TCAGGGAAAT 
CCTGGTGATC CCGGTCCAGT CGGTTACCAA GGAATGAAGG GAGACCAGGG AATAAAAGGT 
GATAAGGGAG AGAGAGGTCA TAAAGGCTTC AAGGGAGACA AAGGTCATCA TGGTCTCGAT 
GGCACGGATG GACGTAAGGG CGAGCCGGGA TTCCCTGGAC TTCCAGGCTG TAAAGGGTCA 
CCAGGGCCAG ATGGTTTGCA AGGAGAATCA GGGCCTAAAG GAGACCCCGG ACAATATGGA 
CGCAAAGGAG AACGAGGAGA TCCTGGCAGA GATGGTGAGC CAGGAAGACC TGGAAACTAC 
GGACCATCTG GGGCCAAGGG AGACAGGGGT CCTCGTGGGG CTAACGGCGA TAAAGGCGAG 
AGAGGAGATG ATGGACCACC AGGACCAGAT GGCCCAAGAG GAGAAAGAGG CCAAGGTGGT 
GAGAAGGGTG AACAAGGGTC TCGAGGTAAC AGAGGTCCCA GAGGCGAGCC TGGTGAACCC 
GGGCCAAGGG GTGAGCAAGG ACGTGAAGGA TCATCCGGAG CAAATGGAGA GCCGGGTGAG 
CAAGGAAGAG CAGGGCAGCC AGGTTATCGT GGCGATGAGG GTCCTGCTGG GCCAGAGGGA 
GATAAAGGGC CCAGAGGAAT CAAAGGGGCT CCAGGAGACA GAGGCCTGAT GGGAGAGAGG 
GGTGAAGACG GACCTCCAGG AAACGGGACG GAGGGTTGTG CTGGCTTTCA GGGTTATCCT 
GGTCCAAGGG GAGATCCTGG AGCACCTGGT GGAAAAGGAA CCCCAGGACC CAAGGGAGAT 
GATGGAGAGC CGGGTGACAC TGGATTAGAT AATGACAGAC TTGGACCACC TGGGACAAAA 
GGAGCCAAAG GTCACAGAGG ACCTGAAGGA AAACCTGGAC CCCCAGGACC ACCCGGACCT 
CAAGGAGCTG ATGAATGTGA GATTCTGGAT ATCATCATGA AGATGTGCTC TTGCTGTGAA 
TGCACATGTG GACCCTTGGA TATTGCCTTT ATCGTGGACA GCTCTGAGAG TATTGGAGCT 
TCTAACTTTG CCATCTCTAA AGAATTCATC GTGACAATAA TGGACAGACT CAAGTTAAGA 
CAGTTTGGTG CCAATGAGTC TCGTATTGGT GTGGTGCAGT ACAGTGGTGA AAAATCTCAA 
GAGGTGGTAC AGCTTGGTGA CCCCAACATC AAGACCCTGA CCGACCTAAA ACAAGCTGTG 
AAAGACTTGC GCTGGCTGGC TGAGGCCACA TACACTGGAG AAGCTCTTCA GTACTCGCTA 
AGAAACATGC TCAGCAAGCT TATCACGGAG AGGAGTGTGG TCCTTGTTCT TACTGATGGA 
CGCTCAGACA CTATTAGGGA TAAAGTGCCA TTAAACGTGC TGTGCGGCAA AGGATTTAAG 
GTTGGTGGGG TTGGAGTGAC GGATTACGCA GAGAGGAAAC CCAATCCGGA ACAGCTTGAT 
GAGGTTGTGT GTAAAGATGA TCCTAGAAAA GGCTTCTCCT TTGTCCTAAA CAACTTTGGC 
CTACTTCTGG ATGACAACTT CCTGCAAAAC CTGACTGAGA AAATCTGTGA AGATAAGAAG 
TGTCCCACAT ATACTTGTCC AATTTCATTT AATGACAACA CCGATGTCCT CATCATGATG 
GACAGCTCGG CCAGCGTTGG CGCCAAGAAC TTCGAGATGA CCAAGGACTT CTCACGGATG 
CTAGCTAAGC GCTTTTTGTC AGCAGAAAGA GGAAATTTCC AGGTTCGAAT TGGTGTTGGC 
CAATACAGCA ACAATGCCAA CTTGGAGGCC GAATTTTCCA CAAACGCCAC TCAGGTGGTC 
GCTCAAATCG CAGACGCCAA GTTCCAGAAT GCAGGTACAC AGGTAACAAA CGCTCTCAAC 
TTCGCCATCG AGCGCTTCAG AGGCGGTAGG ACGAGGAAGA AGAAGCTGCT GGTGTTTTCT 
GATGGTCGTT CTCAAGGTGT TAACTCCATT CAGATTGAGA AAGCCGTGGA GCAGGTGAGC 
AACGCAGGAA TTGAGCTGTA TGTTCTTGCT GTTGGAAATC AGGTGAATGA AGCGCATTTA 
CGCACCTTAG TGAGCCGAGG CCGGCCTTAC GACAACACCT ACGCCTACCG CCATCTTTTC 
AAAGTACCCG ACTACCGCTC ATTGGTCACT GGGGTATTTT ACCAAACTGT GTCCCGCAAG 
ATTTCCCTGG AGACGAGAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304444.1
CDS	164..3187
Translation	MALNGYILTALCALWAGIFAQDVSRSSFRECPVDLFFVLDTSESVALRAKPPEFYINQIKTFTKLFIDELKDLRQPCDRDVTWNSGALHYSDDTELVMGLVDLNTKRADLKAAIDRIKYIGKGTYTDCAIKEGIAELLRAGSHYHENKYIVVVTDGHPITGYKEPCGGIQEAANEARQHAIKVFAVAISPDQEDTRLSVIATDINYRQNFTAADNSRSTQMSTIRSIIHMITNETKDVCCSFDCIADGGPQGPPGDPGQRGETGRPGMPGEKGDFGAQGNPGDPGPVGYQGMKGDQGIKGDKGERGHKGFKGDKGHHGLDGTDGRKGEPGFPGLPGCKGSPGPDGLQGESGPKGDPGQYGRKGERGDPGRDGEPGRPGNYGPSGAKGDRGPRGANGDKGERGDDGPPGPDGPRGERGQGGEKGEQGSRGNRGPRGEPGEPGPRGEQGREGSSGANGEPGEQGRAGQPGYRGDEGPAGPEGDKGPRGIKGAPGDRGLMGERGEDGPPGNGTEGCAGFQGYPGPRGDPGAPGGKGTPGPKGDDGEPGDTGLDNDRLGPPGTKGAKGHRGPEGKPGPPGPPGPQGADECEILDIIMKMCSCCECTCGPLDIAFIVDSSESIGASNFAISKEFIVTIMDRLKLRQFGANESRIGVVQYSGEKSQEVVQLGDPNIKTLTDLKQAVKDLRWLAEATYTGEALQYSLRNMLSKLITERSVVLVLTDGRSDTIRDKVPLNVLCGKGFKVGGVGVTDYAERKPNPEQLDEVVCKDDPRKGFSFVLNNFGLLLDDNFLQNLTEKICEDKKCPTYTCPISFNDNTDVLIMMDSSASVGAKNFEMTKDFSRMLAKRFLSAERGNFQVRIGVGQYSNNANLEAEFSTNATQVVAQIADAKFQNAGTQVTNALNFAIERFRGGRTRKKKLLVFSDGRSQGVNSIQIEKAVEQVSNAGIELYVLAVGNQVNEAHLRTLVSRGRPYDNTYAYRHLFKVPDYRSLVTGVFYQTVSRKISLETRG

Target ORF information:

RefSeq Version	XM_009306169.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type VI, alpha 1 (col6a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306169.2

ORF Insert Sequence:

ATGGCGCTTA ATGGATATAT ATTGACCGCT CTGTGCGCTC TTTGGGCAGG GATCTTCGCT 
CAGGATGTCT CCAGATCCTC CTTCAGAGAG TGTCCTGTGG ACCTGTTCTT TGTGCTGGAC 
ACGTCTGAAA GTGTGGCTCT CAGAGCCAAA CCTCCAGAGT TTTACATCAA CCAGATTAAG 
ACCTTCACAA AGCTGTTCAT CGATGAGCTC AAAGACCTAC GCCAGCCCTG TGATCGCGAT 
GTGACATGGA ACTCGGGAGC GCTGCACTAC AGTGATGACA CAGAGCTGGT CATGGGATTA 
GTCGACCTCA ACACAAAACG TGCTGACCTT AAAGCTGCCA TCGACAGAAT CAAATACATC 
GGCAAGGGAA CGTACACCGA CTGCGCCATC AAGGAGGGCA TCGCTGAGCT GCTCAGAGCG 
GGTTCGCATT ATCATGAGAA TAAATACATT GTGGTGGTGA CGGATGGTCA TCCTATAACC 
GGATATAAGG AACCATGCGG TGGCATTCAG GAGGCCGCCA ATGAGGCTCG ACAACATGCC 
ATTAAAGTGT TTGCTGTGGC CATCTCACCT GACCAGGAGG ACACCAGACT GTCTGTCATT 
GCCACAGATA TAAACTACAG ACAGAACTTC ACCGCTGCAG ACAACTCCCG CTCCACACAA 
ATGAGCACTA TACGCTCCAT CATCCACATG ATTACAAACG AAACAAAGGA TGTGTGCTGC 
TCCTTTGACT GCATCGCTGA TGGAGGCCCT CAAGGACCCC CTGGAGATCC AGGTCAAAGG 
GGAGAGACCG GGAGACCAGG CATGCCAGGA GAAAAAGGAG ACTTTGGGGC TCAGGGAAAT 
CCTGGTGATC CCGGTCCAGT CGGTTACCAA GGAATGAAGG GAGACCAGGG AATAAAAGGT 
GATAAGGGAG AGAGAGGTCA TAAAGGCTTC AAGGGAGACA AAGGTCATCA TGGTCTCGAT 
GGCACGGATG GACGTAAGGG CGAGCCGGGA TTCCCTGGAC TTCCAGGCTG TAAAGGGTCA 
CCAGGGCCAG ATGGTTTGCA AGGAGAATCA GGGCCTAAAG GAGACCCCGG ACAATATGGA 
CGCAAAGGAG AACGAGGAGA TCCTGGCAGA GATGGTGAGC CAGGAAGACC TGGAAACTAC 
GGACCATCTG GGGCCAAGGG AGACAGGGGT CCTCGTGGGG CTAACGGCGA TAAAGGCGAG 
AGAGGAGATG ATGGACCACC AGGACCAGAT GGCCCAAGAG GAGAAAGAGG CCAAGGTGGT 
GAGAAGGGTG AACAAGGGTC TCGAGGTAAC AGAGGTCCCA GAGGCGAGCC TGGTGAACCC 
GGGCCAAGGG GTGAGCAAGG ACGTGAAGGA TCATCCGGAG CAAATGGAGA GCCGGGTGAG 
CAAGGAAGAG CAGGGCAGCC AGGTTATCGT GGCGATGAGG GTCCTGCTGG GCCAGAGGGA 
GATAAAGGGC CCAGAGGAAT CAAAGGGGCT CCAGGAGACA GAGGCCTGAT GGGAGAGAGG 
GGTGAAGACG GACCTCCAGG AAACGGGACG GAGGGTTGTG CTGGCTTTCA GGGTTATCCT 
GGTCCAAGGG GAGATCCTGG AGCACCTGGT GGAAAAGGAA CCCCAGGACC CAAGGGAGAT 
GATGGAGAGC CGGGTGACAC TGGATTAGAT AATGACAGAC TTGGACCACC TGGGACAAAA 
GGAGCCAAAG GTCACAGAGG ACCTGAAGGA AAACCTGGAC CCCCAGGACC ACCCGGACCT 
CAAGGAGCTG ATGAATGTGA GATTCTGGAT ATCATCATGA AGATGTGCTC TTGCTGTGAA 
TGCACATGTG GACCCTTGGA TATTGCCTTT ATCGTGGACA GCTCTGAGAG TATTGGAGCT 
TCTAACTTTG CCATCTCTAA AGAATTCATC GTGACAATAA TGGACAGACT CAAGTTAAGA 
CAGTTTGGTG CCAATGAGTC TCGTATTGGT GTGGTGCAGT ACAGTGGTGA AAAATCTCAA 
GAGGTGGTAC AGCTTGGTGA CCCCAACATC AAGACCCTGA CCGACCTAAA ACAAGCTGTG 
AAAGACTTGC GCTGGCTGGC TGAGGCCACA TACACTGGAG AAGCTCTTCA GTACTCGCTA 
AGAAACATGC TCAGCAAGCT TATCACGGAG AGGAGTGTGG TCCTTGTTCT TACTGATGGA 
CGCTCAGACA CTATTAGGGA TAAAGTGCCA TTAAACGTGC TGTGCGGCAA AGGATTTAAG 
GTTGGTGGGG TTGGAGTGAC GGATTACGCA GAGAGGAAAC CCAATCCGGA ACAGCTTGAT 
GAGGTTGTGT GTAAAGATGA TCCTAGAAAA GGCTTCTCCT TTGTCCTAAA CAACTTTGGC 
CTACTTCTGG ATGACAACTT CCTGCAAAAC CTGACTGAGA AAATCTGTGA AGATAAGAAG 
TGTCCCACAT ATACTTGTCC AATTTCATTT AATGACAACA CCGATGTCCT CATCATGATG 
GACAGCTCGG CCAGCGTTGG CGCCAAGAAC TTCGAGATGA CCAAGGACTT CTCACGGATG 
CTAGCTAAGC GCTTTTTGTC AGCAGAAAGA GGAAATTTCC AGGTTCGAAT TGGTGTTGGC 
CAATACAGCA ACAATGCCAA CTTGGAGGCC GAATTTTCCA CAAACGCCAC TCAGGTGGTC 
GCTCAAATCG CAGACGCCAA GTTCCAGAAT GCAGGTACAC AGGTAACAAA CGCTCTCAAC 
TTCGCCATCG AGCGCTTCAG AGGCGGTAGG ACGAGGAAGA AGAAGCTGCT GGTGTTTTCT 
GATGGTCGTT CTCAAGGTGT TAACTCCATT CAGATTGAGA AAGCCGTGGA GCAGGTGAGC 
AACGCAGGAA TTGAGCTGTA TGTTCTTGCT GTTGGAAATC AGGTGAATGA AGCGCATTTA 
CGCACCTTAG TGAGCCGAGG CCGGCCTTAC GACAACACCT ACGCCTACCG CCATCTTTTC 
AAAGTACCCG ACTACCGCTC ATTGGTCACT GGGGTATTTT ACCAAACTGT GTCCCGCAAG 
ATTTCCCTGG AGACGAGAGG GTGA