naa25 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	naa25
Entrez Gene ID	568789
Full Name	N(alpha)-acetyltransferase 25, NatB auxiliary subunit
Synonyms	im:7143469,im:7156224,si:ch73-149o12.1
General protein information	Preferred Names N(alpha)-acetyltransferase 25, NatB auxiliary subunit Names N-alpha-acetyltransferase 25, NatB auxiliary subunit
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005172598.3	XP_005172655.1	N-alpha-acetyltransferase 25, NatB auxiliary subunit
XM_005172598.4	XP_005172655.1	N-alpha-acetyltransferase 25, NatB auxiliary subunit

Pathways from BioSystems

Homologies

	NAA25	XP_001102995.2
Bos taurus (cattle)	NAA25	NP_001193056.1
Danio rerio (zebrafish)	naa25	XP_005172656.1
Anopheles gambiae (African malaria mosquito)	NAA25_ANOGA	XP_321109.5
Caenorhabditis elegans (roundworm)	cra-1	NP_498494.2
Schizosaccharomyces pombe (fission yeast)	naa25	NP_595632.1
Xenopus tropicalis (tropical clawed frog)	naa25	XP_004910562.1
Pan troglodytes (chimpanzee)	NAA25	XP_509384.2
Canis lupus familiaris (dog)	NAA25	XP_543400.1
Drosophila melanogaster (fruit fly)	psidin	NP_650858.1
Homo sapiens (human)	NAA25	NP_079229.2
Mus musculus (house mouse)	Naa25	NP_766310.2
Rattus norvegicus (Norway rat)	Naa25	NP_001041365.1
Gallus gallus (chicken)	NAA25	NP_001025835.2

Gene Ontology

The following naa25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the naa25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa15709	XM_005172598.4 Latest version!	Danio rerio N(alpha)-acetyltransferase 25, NatB auxiliary subunit (naa25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa15709	XM_005172598.3	Danio rerio N(alpha)-acetyltransferase 25, NatB auxiliary subunit (naa25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

naa25 ( XM_005172598.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172655.1 Danio rerio N(alpha)-acetyltransferase 25, NatB auxiliary subunit (naa25), mRNA.

Price & Availability↑

CloneID	ODa15709
Clone ID Related Accession (Same CDS sequence)	XM_005172598.3 , XM_005172598.4
Accession Version	XM_005172598.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	N-alpha-acetyltransferase 25, NatB auxiliary subunit
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172598.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA GGGGCCATGT GCAAGACCCT AACGACAGGA GGCTACGACC TATCTACGAT 
TACCTGGACA ATGGAAATAA CAAGATGGCA ATCCAGCAGG CCGACAAACT GCTCAAGAAG 
CACAAAGATC TCCACTGCGC AAAGGTCCTG AAGGCAATCG GTCTGCAGCG AACAGGAAAA 
CAGGATGAAG CCTTTACGTT AGCACAGGAA GTGGCGGTTC TCGAACCCAC TGATGACAAC 
TCTCTACAGG CACTGACCAT CCTCTACAGA GAGATGCACC GACCCGAGTT AGTGACCAAG 
CTTTATGAAG CTGCAGTTCG GAAGGTTCCA GCAAGCGAAG AGTACCACTC TCACCTCTTC 
ATGGCCTATG CTCGGGTTGG AGAATACAAG AAGATGCAAC AGGCTGGAAT GGCACTCTAT 
AAAATTGTCC CCAAAAACCC CTATTACTTC TGGTCAGTCA TGAGCTTGGT GATGCAGGCC 
ATCTCAGCGC AAGACGAGAA GCTCTCTCAG ACCATGTTCC TGCCATTAGC AGAGCGTATG 
GTGGAGAAAA TGGTCAAGGA GGAAAAAATC GAGGCAGAAG CTGAGGTTCA GCTGTACTTC 
ATGATCTTGG AGCGCTTGGG AAAATATGTG GAGGCGCTGG AGGTGGTTCA GGGGCCACTC 
GGAGAGAAGT TGACCAGTGA ACTACAGAGC CGTGAGAACA AATGTATGAA GTTGTACCGG 
CGTCTGGAGC GCTGGCCCGA GTGCAACGCT TTGTCCAGCA AACTGCTCCT GAAAAACCCT 
GATGACTGGC AGTTCTACTT GTTGTATTTT GACTCGTTGT TCCACCTCAT CGATCAAAGC 
TGGACGCCGC CACAGGAAGG AGCTCACTGT GCAGAAGGTG AAGTTCATGC CTCTGTAGCT 
CAGTCCATCA GCTTCATGGA GGAGCGTCTG GCCACAGAAG ACGCAAAGGA GTCGAAACAT 
CTGAGAGGAC CATACCTGGC CCATCTGGAG CTCATCAGAC GTCTCCGAGA GCGCAGCTGT 
CCTGAAGCAC AGCAGCTCGG GGATCCTCTT GAGCTTATGT TCCAGTTCTT CGTGAAGTTT 
GGAGACAAGC CATGTTGCAT CACGGACCTG AAGATCTTTC TGGACCTTCT CATCCCTGAC 
CAACATGTGC AGTTCATCAA CAGGCTAATG GAGGCTGTGC CACTGGGTGC TCCAGGAGAG 
GATGGTTTCG CCTTCCCAGA AGACACTCGG GCTCTGCAGA GACACATGTG TGTGACGCAG 
CTCAGCCGCG GTCTGGGTCT ACAGCATTCC CTCAACACTG ACGGCAAACT GGGGGTCATT 
AAAGAGCTGA AGGCACACTA CCGGCACGGC TTACAGTTCG GGAAGTCCTG TTTAAAAACG 
GAGCTGCAGT TCTCGGATAT GTACTGTCTC ATGGCAGCGC ATGTCTATAT AGACCTGTGG 
ATTGAGACGG ATGATCAGAA CATGTTGTGG CAGTGTCTGG GGATGTTAGA AGAAGGTCTG 
TCCCACAGTT CCTCCAACGC TCAATTCAAA CTGCTGCTTT TGCTTCTGTA CTGCCGTCTT 
GGGGCTTTTG AGCCGGTGGT AGATCTTTAC TCCAGTTTGG ATGCTAAACA CGTCCAGCAT 
GACACCATTG GGTATTTATT GACCCGCTAT GCAGAGTCTT TGGGCCAGTT TGCTGCTGCT 
TCCCAATCTT GTAACTTCTC CCTCAGGTTT TTTCACTCGA ACCAGAAAGA TACCTCAGAA 
TACATTATTC AAGCATATAA GTACGGTGCT TTTGAGAAAA TCCCGGAGTT CATCGCCTTC 
AGGAACCGAC TCAATCACTC GCTGCACTTT GCTCAAGTGC GCACGGAGCG GATGCTGCTG 
GACCTCTTCC TAGAAGCCGA CATATCTTCA ACTCTAGACG AGAGCGTAAA ATCGATGTCT 
TTGTGTCCGG AGGAAGATGA TATCCCATGG GACAATTTAA GGGACAACCG TGACCTGACT 
GTCTTTGTCA GCTGGGACCC TAAAGACAAG CAGCTGAAGG AGGAGCACAA GCTGCGCTCT 
CTAGAAGACG AGACCCTGTG GTTGAAGCTG CGGTCGCTCA CTCTGCGTCT GATTGGCTGC 
GTTTCTGGAC TCACTCACTC GTCGACGGCA CGCAACTCTG AAAAGACAAC TGAAAACGGA 
GTCACGGCCA AACCATCTTC TCTACAATCT CTGCTCTCCC AGCTAGAAAA CACACTAAAC 
CAGGCCTCGC AGTTCACGGA AAAACAGCTT CAGCAGCAGT ACCCGTTTTT GGGTCCCGTG 
TCGTCTCGTT TGTCTCAGGC TGTGTCCAGT GGCTGCTGTC AATGTCAGCT GTCGTCTCTT 
CAGCTTCCTC TGCACCTGCA AGAGCTGGAA ACTATTGACC TAGATGAATC AACAGAACTT 
CAAACACAAA TATCAAATCT TTTCAAGTCT TTAGCAGTAC AACTTCAAGA AATGTTGGAA 
AAATGTAAAG GAGATCTATT AGAGGTGAAA GACGGTCAAA CCAAGACGCA GCACTTTTTA 
CTGGAGCATC TAGTCTACTT TGTTGAAACT GTTTGTATCG TCCTATGGAT ATCTAGTTAC 
TGTGCAAGTG TCCTCCGGCC CTTAAAGTCC AGTTTACAGA AGAAGAAGAA AAAGAAAAAA 
GAAGCTAGCA TCGTGACGCC GGCGGTGATT TTGGCCTTTC AGGAGTTTAG CAGCAGCTTG 
CAGAGCCTCC TCAACCAGGC CCTGGAGCAC ATCAAGAGCC AGGAGATCAG CCTGACCGCA 
CTGAAGCTGG AGGCCCTCAG TCTGGAGGGA CAAACCCAGA CCGAGGCGGA AGGGACCTTC 
ACAAAGACCG CGATGGAGAA GGTGCAGAGC AGCTACCTGC GTTCGCTGCA AGAGATCGGG 
GAACTGTTGA AGAAGAGAGC GGACTCGTTG AAATCCCTCA AAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172655.1
CDS	29..2956
Translation	MAARGHVQDPNDRRLRPIYDYLDNGNNKMAIQQADKLLKKHKDLHCAKVLKAIGLQRTGKQDEAFTLAQEVAVLEPTDDNSLQALTILYREMHRPELVTKLYEAAVRKVPASEEYHSHLFMAYARVGEYKKMQQAGMALYKIVPKNPYYFWSVMSLVMQAISAQDEKLSQTMFLPLAERMVEKMVKEEKIEAEAEVQLYFMILERLGKYVEALEVVQGPLGEKLTSELQSRENKCMKLYRRLERWPECNALSSKLLLKNPDDWQFYLLYFDSLFHLIDQSWTPPQEGAHCAEGEVHASVAQSISFMEERLATEDAKESKHLRGPYLAHLELIRRLRERSCPEAQQLGDPLELMFQFFVKFGDKPCCITDLKIFLDLLIPDQHVQFINRLMEAVPLGAPGEDGFAFPEDTRALQRHMCVTQLSRGLGLQHSLNTDGKLGVIKELKAHYRHGLQFGKSCLKTELQFSDMYCLMAAHVYIDLWIETDDQNMLWQCLGMLEEGLSHSSSNAQFKLLLLLLYCRLGAFEPVVDLYSSLDAKHVQHDTIGYLLTRYAESLGQFAAASQSCNFSLRFFHSNQKDTSEYIIQAYKYGAFEKIPEFIAFRNRLNHSLHFAQVRTERMLLDLFLEADISSTLDESVKSMSLCPEEDDIPWDNLRDNRDLTVFVSWDPKDKQLKEEHKLRSLEDETLWLKLRSLTLRLIGCVSGLTHSSTARNSEKTTENGVTAKPSSLQSLLSQLENTLNQASQFTEKQLQQQYPFLGPVSSRLSQAVSSGCCQCQLSSLQLPLHLQELETIDLDESTELQTQISNLFKSLAVQLQEMLEKCKGDLLEVKDGQTKTQHFLLEHLVYFVETVCIVLWISSYCASVLRPLKSSLQKKKKKKKEASIVTPAVILAFQEFSSSLQSLLNQALEHIKSQEISLTALKLEALSLEGQTQTEAEGTFTKTAMEKVQSSYLRSLQEIGELLKKRADSLKSLKI

Target ORF information:

RefSeq Version	XM_005172598.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio N(alpha)-acetyltransferase 25, NatB auxiliary subunit (naa25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172598.3

ORF Insert Sequence:

ATGGCGGCGA GGGGCCATGT GCAAGACCCT AACGACAGGA GGCTACGACC TATCTACGAT 
TACCTGGACA ATGGAAATAA CAAGATGGCA ATCCAGCAGG CCGACAAACT GCTCAAGAAG 
CACAAAGATC TCCACTGCGC AAAGGTCCTG AAGGCAATCG GTCTGCAGCG AACAGGAAAA 
CAGGATGAAG CCTTTACGTT AGCACAGGAA GTGGCGGTTC TCGAACCCAC TGATGACAAC 
TCTCTACAGG CACTGACCAT CCTCTACAGA GAGATGCACC GACCCGAGTT AGTGACCAAG 
CTTTATGAAG CTGCAGTTCG GAAGGTTCCA GCAAGCGAAG AGTACCACTC TCACCTCTTC 
ATGGCCTATG CTCGGGTTGG AGAATACAAG AAGATGCAAC AGGCTGGAAT GGCACTCTAT 
AAAATTGTCC CCAAAAACCC CTATTACTTC TGGTCAGTCA TGAGCTTGGT GATGCAGGCC 
ATCTCAGCGC AAGACGAGAA GCTCTCTCAG ACCATGTTCC TGCCATTAGC AGAGCGTATG 
GTGGAGAAAA TGGTCAAGGA GGAAAAAATC GAGGCAGAAG CTGAGGTTCA GCTGTACTTC 
ATGATCTTGG AGCGCTTGGG AAAATATGTG GAGGCGCTGG AGGTGGTTCA GGGGCCACTC 
GGAGAGAAGT TGACCAGTGA ACTACAGAGC CGTGAGAACA AATGTATGAA GTTGTACCGG 
CGTCTGGAGC GCTGGCCCGA GTGCAACGCT TTGTCCAGCA AACTGCTCCT GAAAAACCCT 
GATGACTGGC AGTTCTACTT GTTGTATTTT GACTCGTTGT TCCACCTCAT CGATCAAAGC 
TGGACGCCGC CACAGGAAGG AGCTCACTGT GCAGAAGGTG AAGTTCATGC CTCTGTAGCT 
CAGTCCATCA GCTTCATGGA GGAGCGTCTG GCCACAGAAG ACGCAAAGGA GTCGAAACAT 
CTGAGAGGAC CATACCTGGC CCATCTGGAG CTCATCAGAC GTCTCCGAGA GCGCAGCTGT 
CCTGAAGCAC AGCAGCTCGG GGATCCTCTT GAGCTTATGT TCCAGTTCTT CGTGAAGTTT 
GGAGACAAGC CATGTTGCAT CACGGACCTG AAGATCTTTC TGGACCTTCT CATCCCTGAC 
CAACATGTGC AGTTCATCAA CAGGCTAATG GAGGCTGTGC CACTGGGTGC TCCAGGAGAG 
GATGGTTTCG CCTTCCCAGA AGACACTCGG GCTCTGCAGA GACACATGTG TGTGACGCAG 
CTCAGCCGCG GTCTGGGTCT ACAGCATTCC CTCAACACTG ACGGCAAACT GGGGGTCATT 
AAAGAGCTGA AGGCACACTA CCGGCACGGC TTACAGTTCG GGAAGTCCTG TTTAAAAACG 
GAGCTGCAGT TCTCGGATAT GTACTGTCTC ATGGCAGCGC ATGTCTATAT AGACCTGTGG 
ATTGAGACGG ATGATCAGAA CATGTTGTGG CAGTGTCTGG GGATGTTAGA AGAAGGTCTG 
TCCCACAGTT CCTCCAACGC TCAATTCAAA CTGCTGCTTT TGCTTCTGTA CTGCCGTCTT 
GGGGCTTTTG AGCCGGTGGT AGATCTTTAC TCCAGTTTGG ATGCTAAACA CGTCCAGCAT 
GACACCATTG GGTATTTATT GACCCGCTAT GCAGAGTCTT TGGGCCAGTT TGCTGCTGCT 
TCCCAATCTT GTAACTTCTC CCTCAGGTTT TTTCACTCGA ACCAGAAAGA TACCTCAGAA 
TACATTATTC AAGCATATAA GTACGGTGCT TTTGAGAAAA TCCCGGAGTT CATCGCCTTC 
AGGAACCGAC TCAATCACTC GCTGCACTTT GCTCAAGTGC GCACGGAGCG GATGCTGCTG 
GACCTCTTCC TAGAAGCCGA CATATCTTCA ACTCTAGACG AGAGCGTAAA ATCGATGTCT 
TTGTGTCCGG AGGAAGATGA TATCCCATGG GACAATTTAA GGGACAACCG TGACCTGACT 
GTCTTTGTCA GCTGGGACCC TAAAGACAAG CAGCTGAAGG AGGAGCACAA GCTGCGCTCT 
CTAGAAGACG AGACCCTGTG GTTGAAGCTG CGGTCGCTCA CTCTGCGTCT GATTGGCTGC 
GTTTCTGGAC TCACTCACTC GTCGACGGCA CGCAACTCTG AAAAGACAAC TGAAAACGGA 
GTCACGGCCA AACCATCTTC TCTACAATCT CTGCTCTCCC AGCTAGAAAA CACACTAAAC 
CAGGCCTCGC AGTTCACGGA AAAACAGCTT CAGCAGCAGT ACCCGTTTTT GGGTCCCGTG 
TCGTCTCGTT TGTCTCAGGC TGTGTCCAGT GGCTGCTGTC AATGTCAGCT GTCGTCTCTT 
CAGCTTCCTC TGCACCTGCA AGAGCTGGAA ACTATTGACC TAGATGAATC AACAGAACTT 
CAAACACAAA TATCAAATCT TTTCAAGTCT TTAGCAGTAC AACTTCAAGA AATGTTGGAA 
AAATGTAAAG GAGATCTATT AGAGGTGAAA GACGGTCAAA CCAAGACGCA GCACTTTTTA 
CTGGAGCATC TAGTCTACTT TGTTGAAACT GTTTGTATCG TCCTATGGAT ATCTAGTTAC 
TGTGCAAGTG TCCTCCGGCC CTTAAAGTCC AGTTTACAGA AGAAGAAGAA AAAGAAAAAA 
GAAGCTAGCA TCGTGACGCC GGCGGTGATT TTGGCCTTTC AGGAGTTTAG CAGCAGCTTG 
CAGAGCCTCC TCAACCAGGC CCTGGAGCAC ATCAAGAGCC AGGAGATCAG CCTGACCGCA 
CTGAAGCTGG AGGCCCTCAG TCTGGAGGGA CAAACCCAGA CCGAGGCGGA AGGGACCTTC 
ACAAAGACCG CGATGGAGAA GGTGCAGAGC AGCTACCTGC GTTCGCTGCA AGAGATCGGG 
GAACTGTTGA AGAAGAGAGC GGACTCGTTG AAATCCCTCA AAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

naa25 ( XM_005172598.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172655.1 Danio rerio N(alpha)-acetyltransferase 25, NatB auxiliary subunit (naa25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15709
Clone ID Related Accession (Same CDS sequence)	XM_005172598.3 , XM_005172598.4
Accession Version	XM_005172598.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	N-alpha-acetyltransferase 25, NatB auxiliary subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172598.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA GGGGCCATGT GCAAGACCCT AACGACAGGA GGCTACGACC TATCTACGAT 
TACCTGGACA ATGGAAATAA CAAGATGGCA ATCCAGCAGG CCGACAAACT GCTCAAGAAG 
CACAAAGATC TCCACTGCGC AAAGGTCCTG AAGGCAATCG GTCTGCAGCG AACAGGAAAA 
CAGGATGAAG CCTTTACGTT AGCACAGGAA GTGGCGGTTC TCGAACCCAC TGATGACAAC 
TCTCTACAGG CACTGACCAT CCTCTACAGA GAGATGCACC GACCCGAGTT AGTGACCAAG 
CTTTATGAAG CTGCAGTTCG GAAGGTTCCA GCAAGCGAAG AGTACCACTC TCACCTCTTC 
ATGGCCTATG CTCGGGTTGG AGAATACAAG AAGATGCAAC AGGCTGGAAT GGCACTCTAT 
AAAATTGTCC CCAAAAACCC CTATTACTTC TGGTCAGTCA TGAGCTTGGT GATGCAGGCC 
ATCTCAGCGC AAGACGAGAA GCTCTCTCAG ACCATGTTCC TGCCATTAGC AGAGCGTATG 
GTGGAGAAAA TGGTCAAGGA GGAAAAAATC GAGGCAGAAG CTGAGGTTCA GCTGTACTTC 
ATGATCTTGG AGCGCTTGGG AAAATATGTG GAGGCGCTGG AGGTGGTTCA GGGGCCACTC 
GGAGAGAAGT TGACCAGTGA ACTACAGAGC CGTGAGAACA AATGTATGAA GTTGTACCGG 
CGTCTGGAGC GCTGGCCCGA GTGCAACGCT TTGTCCAGCA AACTGCTCCT GAAAAACCCT 
GATGACTGGC AGTTCTACTT GTTGTATTTT GACTCGTTGT TCCACCTCAT CGATCAAAGC 
TGGACGCCGC CACAGGAAGG AGCTCACTGT GCAGAAGGTG AAGTTCATGC CTCTGTAGCT 
CAGTCCATCA GCTTCATGGA GGAGCGTCTG GCCACAGAAG ACGCAAAGGA GTCGAAACAT 
CTGAGAGGAC CATACCTGGC CCATCTGGAG CTCATCAGAC GTCTCCGAGA GCGCAGCTGT 
CCTGAAGCAC AGCAGCTCGG GGATCCTCTT GAGCTTATGT TCCAGTTCTT CGTGAAGTTT 
GGAGACAAGC CATGTTGCAT CACGGACCTG AAGATCTTTC TGGACCTTCT CATCCCTGAC 
CAACATGTGC AGTTCATCAA CAGGCTAATG GAGGCTGTGC CACTGGGTGC TCCAGGAGAG 
GATGGTTTCG CCTTCCCAGA AGACACTCGG GCTCTGCAGA GACACATGTG TGTGACGCAG 
CTCAGCCGCG GTCTGGGTCT ACAGCATTCC CTCAACACTG ACGGCAAACT GGGGGTCATT 
AAAGAGCTGA AGGCACACTA CCGGCACGGC TTACAGTTCG GGAAGTCCTG TTTAAAAACG 
GAGCTGCAGT TCTCGGATAT GTACTGTCTC ATGGCAGCGC ATGTCTATAT AGACCTGTGG 
ATTGAGACGG ATGATCAGAA CATGTTGTGG CAGTGTCTGG GGATGTTAGA AGAAGGTCTG 
TCCCACAGTT CCTCCAACGC TCAATTCAAA CTGCTGCTTT TGCTTCTGTA CTGCCGTCTT 
GGGGCTTTTG AGCCGGTGGT AGATCTTTAC TCCAGTTTGG ATGCTAAACA CGTCCAGCAT 
GACACCATTG GGTATTTATT GACCCGCTAT GCAGAGTCTT TGGGCCAGTT TGCTGCTGCT 
TCCCAATCTT GTAACTTCTC CCTCAGGTTT TTTCACTCGA ACCAGAAAGA TACCTCAGAA 
TACATTATTC AAGCATATAA GTACGGTGCT TTTGAGAAAA TCCCGGAGTT CATCGCCTTC 
AGGAACCGAC TCAATCACTC GCTGCACTTT GCTCAAGTGC GCACGGAGCG GATGCTGCTG 
GACCTCTTCC TAGAAGCCGA CATATCTTCA ACTCTAGACG AGAGCGTAAA ATCGATGTCT 
TTGTGTCCGG AGGAAGATGA TATCCCATGG GACAATTTAA GGGACAACCG TGACCTGACT 
GTCTTTGTCA GCTGGGACCC TAAAGACAAG CAGCTGAAGG AGGAGCACAA GCTGCGCTCT 
CTAGAAGACG AGACCCTGTG GTTGAAGCTG CGGTCGCTCA CTCTGCGTCT GATTGGCTGC 
GTTTCTGGAC TCACTCACTC GTCGACGGCA CGCAACTCTG AAAAGACAAC TGAAAACGGA 
GTCACGGCCA AACCATCTTC TCTACAATCT CTGCTCTCCC AGCTAGAAAA CACACTAAAC 
CAGGCCTCGC AGTTCACGGA AAAACAGCTT CAGCAGCAGT ACCCGTTTTT GGGTCCCGTG 
TCGTCTCGTT TGTCTCAGGC TGTGTCCAGT GGCTGCTGTC AATGTCAGCT GTCGTCTCTT 
CAGCTTCCTC TGCACCTGCA AGAGCTGGAA ACTATTGACC TAGATGAATC AACAGAACTT 
CAAACACAAA TATCAAATCT TTTCAAGTCT TTAGCAGTAC AACTTCAAGA AATGTTGGAA 
AAATGTAAAG GAGATCTATT AGAGGTGAAA GACGGTCAAA CCAAGACGCA GCACTTTTTA 
CTGGAGCATC TAGTCTACTT TGTTGAAACT GTTTGTATCG TCCTATGGAT ATCTAGTTAC 
TGTGCAAGTG TCCTCCGGCC CTTAAAGTCC AGTTTACAGA AGAAGAAGAA AAAGAAAAAA 
GAAGCTAGCA TCGTGACGCC GGCGGTGATT TTGGCCTTTC AGGAGTTTAG CAGCAGCTTG 
CAGAGCCTCC TCAACCAGGC CCTGGAGCAC ATCAAGAGCC AGGAGATCAG CCTGACCGCA 
CTGAAGCTGG AGGCCCTCAG TCTGGAGGGA CAAACCCAGA CCGAGGCGGA AGGGACCTTC 
ACAAAGACCG CGATGGAGAA GGTGCAGAGC AGCTACCTGC GTTCGCTGCA AGAGATCGGG 
GAACTGTTGA AGAAGAGAGC GGACTCGTTG AAATCCCTCA AAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172655.1
CDS	43..2970
Translation	MAARGHVQDPNDRRLRPIYDYLDNGNNKMAIQQADKLLKKHKDLHCAKVLKAIGLQRTGKQDEAFTLAQEVAVLEPTDDNSLQALTILYREMHRPELVTKLYEAAVRKVPASEEYHSHLFMAYARVGEYKKMQQAGMALYKIVPKNPYYFWSVMSLVMQAISAQDEKLSQTMFLPLAERMVEKMVKEEKIEAEAEVQLYFMILERLGKYVEALEVVQGPLGEKLTSELQSRENKCMKLYRRLERWPECNALSSKLLLKNPDDWQFYLLYFDSLFHLIDQSWTPPQEGAHCAEGEVHASVAQSISFMEERLATEDAKESKHLRGPYLAHLELIRRLRERSCPEAQQLGDPLELMFQFFVKFGDKPCCITDLKIFLDLLIPDQHVQFINRLMEAVPLGAPGEDGFAFPEDTRALQRHMCVTQLSRGLGLQHSLNTDGKLGVIKELKAHYRHGLQFGKSCLKTELQFSDMYCLMAAHVYIDLWIETDDQNMLWQCLGMLEEGLSHSSSNAQFKLLLLLLYCRLGAFEPVVDLYSSLDAKHVQHDTIGYLLTRYAESLGQFAAASQSCNFSLRFFHSNQKDTSEYIIQAYKYGAFEKIPEFIAFRNRLNHSLHFAQVRTERMLLDLFLEADISSTLDESVKSMSLCPEEDDIPWDNLRDNRDLTVFVSWDPKDKQLKEEHKLRSLEDETLWLKLRSLTLRLIGCVSGLTHSSTARNSEKTTENGVTAKPSSLQSLLSQLENTLNQASQFTEKQLQQQYPFLGPVSSRLSQAVSSGCCQCQLSSLQLPLHLQELETIDLDESTELQTQISNLFKSLAVQLQEMLEKCKGDLLEVKDGQTKTQHFLLEHLVYFVETVCIVLWISSYCASVLRPLKSSLQKKKKKKKEASIVTPAVILAFQEFSSSLQSLLNQALEHIKSQEISLTALKLEALSLEGQTQTEAEGTFTKTAMEKVQSSYLRSLQEIGELLKKRADSLKSLKI

Target ORF information:

RefSeq Version	XM_005172598.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N(alpha)-acetyltransferase 25, NatB auxiliary subunit (naa25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172598.4

ORF Insert Sequence:

ATGGCGGCGA GGGGCCATGT GCAAGACCCT AACGACAGGA GGCTACGACC TATCTACGAT 
TACCTGGACA ATGGAAATAA CAAGATGGCA ATCCAGCAGG CCGACAAACT GCTCAAGAAG 
CACAAAGATC TCCACTGCGC AAAGGTCCTG AAGGCAATCG GTCTGCAGCG AACAGGAAAA 
CAGGATGAAG CCTTTACGTT AGCACAGGAA GTGGCGGTTC TCGAACCCAC TGATGACAAC 
TCTCTACAGG CACTGACCAT CCTCTACAGA GAGATGCACC GACCCGAGTT AGTGACCAAG 
CTTTATGAAG CTGCAGTTCG GAAGGTTCCA GCAAGCGAAG AGTACCACTC TCACCTCTTC 
ATGGCCTATG CTCGGGTTGG AGAATACAAG AAGATGCAAC AGGCTGGAAT GGCACTCTAT 
AAAATTGTCC CCAAAAACCC CTATTACTTC TGGTCAGTCA TGAGCTTGGT GATGCAGGCC 
ATCTCAGCGC AAGACGAGAA GCTCTCTCAG ACCATGTTCC TGCCATTAGC AGAGCGTATG 
GTGGAGAAAA TGGTCAAGGA GGAAAAAATC GAGGCAGAAG CTGAGGTTCA GCTGTACTTC 
ATGATCTTGG AGCGCTTGGG AAAATATGTG GAGGCGCTGG AGGTGGTTCA GGGGCCACTC 
GGAGAGAAGT TGACCAGTGA ACTACAGAGC CGTGAGAACA AATGTATGAA GTTGTACCGG 
CGTCTGGAGC GCTGGCCCGA GTGCAACGCT TTGTCCAGCA AACTGCTCCT GAAAAACCCT 
GATGACTGGC AGTTCTACTT GTTGTATTTT GACTCGTTGT TCCACCTCAT CGATCAAAGC 
TGGACGCCGC CACAGGAAGG AGCTCACTGT GCAGAAGGTG AAGTTCATGC CTCTGTAGCT 
CAGTCCATCA GCTTCATGGA GGAGCGTCTG GCCACAGAAG ACGCAAAGGA GTCGAAACAT 
CTGAGAGGAC CATACCTGGC CCATCTGGAG CTCATCAGAC GTCTCCGAGA GCGCAGCTGT 
CCTGAAGCAC AGCAGCTCGG GGATCCTCTT GAGCTTATGT TCCAGTTCTT CGTGAAGTTT 
GGAGACAAGC CATGTTGCAT CACGGACCTG AAGATCTTTC TGGACCTTCT CATCCCTGAC 
CAACATGTGC AGTTCATCAA CAGGCTAATG GAGGCTGTGC CACTGGGTGC TCCAGGAGAG 
GATGGTTTCG CCTTCCCAGA AGACACTCGG GCTCTGCAGA GACACATGTG TGTGACGCAG 
CTCAGCCGCG GTCTGGGTCT ACAGCATTCC CTCAACACTG ACGGCAAACT GGGGGTCATT 
AAAGAGCTGA AGGCACACTA CCGGCACGGC TTACAGTTCG GGAAGTCCTG TTTAAAAACG 
GAGCTGCAGT TCTCGGATAT GTACTGTCTC ATGGCAGCGC ATGTCTATAT AGACCTGTGG 
ATTGAGACGG ATGATCAGAA CATGTTGTGG CAGTGTCTGG GGATGTTAGA AGAAGGTCTG 
TCCCACAGTT CCTCCAACGC TCAATTCAAA CTGCTGCTTT TGCTTCTGTA CTGCCGTCTT 
GGGGCTTTTG AGCCGGTGGT AGATCTTTAC TCCAGTTTGG ATGCTAAACA CGTCCAGCAT 
GACACCATTG GGTATTTATT GACCCGCTAT GCAGAGTCTT TGGGCCAGTT TGCTGCTGCT 
TCCCAATCTT GTAACTTCTC CCTCAGGTTT TTTCACTCGA ACCAGAAAGA TACCTCAGAA 
TACATTATTC AAGCATATAA GTACGGTGCT TTTGAGAAAA TCCCGGAGTT CATCGCCTTC 
AGGAACCGAC TCAATCACTC GCTGCACTTT GCTCAAGTGC GCACGGAGCG GATGCTGCTG 
GACCTCTTCC TAGAAGCCGA CATATCTTCA ACTCTAGACG AGAGCGTAAA ATCGATGTCT 
TTGTGTCCGG AGGAAGATGA TATCCCATGG GACAATTTAA GGGACAACCG TGACCTGACT 
GTCTTTGTCA GCTGGGACCC TAAAGACAAG CAGCTGAAGG AGGAGCACAA GCTGCGCTCT 
CTAGAAGACG AGACCCTGTG GTTGAAGCTG CGGTCGCTCA CTCTGCGTCT GATTGGCTGC 
GTTTCTGGAC TCACTCACTC GTCGACGGCA CGCAACTCTG AAAAGACAAC TGAAAACGGA 
GTCACGGCCA AACCATCTTC TCTACAATCT CTGCTCTCCC AGCTAGAAAA CACACTAAAC 
CAGGCCTCGC AGTTCACGGA AAAACAGCTT CAGCAGCAGT ACCCGTTTTT GGGTCCCGTG 
TCGTCTCGTT TGTCTCAGGC TGTGTCCAGT GGCTGCTGTC AATGTCAGCT GTCGTCTCTT 
CAGCTTCCTC TGCACCTGCA AGAGCTGGAA ACTATTGACC TAGATGAATC AACAGAACTT 
CAAACACAAA TATCAAATCT TTTCAAGTCT TTAGCAGTAC AACTTCAAGA AATGTTGGAA 
AAATGTAAAG GAGATCTATT AGAGGTGAAA GACGGTCAAA CCAAGACGCA GCACTTTTTA 
CTGGAGCATC TAGTCTACTT TGTTGAAACT GTTTGTATCG TCCTATGGAT ATCTAGTTAC 
TGTGCAAGTG TCCTCCGGCC CTTAAAGTCC AGTTTACAGA AGAAGAAGAA AAAGAAAAAA 
GAAGCTAGCA TCGTGACGCC GGCGGTGATT TTGGCCTTTC AGGAGTTTAG CAGCAGCTTG 
CAGAGCCTCC TCAACCAGGC CCTGGAGCAC ATCAAGAGCC AGGAGATCAG CCTGACCGCA 
CTGAAGCTGG AGGCCCTCAG TCTGGAGGGA CAAACCCAGA CCGAGGCGGA AGGGACCTTC 
ACAAAGACCG CGATGGAGAA GGTGCAGAGC AGCTACCTGC GTTCGCTGCA AGAGATCGGG 
GAACTGTTGA AGAAGAGAGC GGACTCGTTG AAATCCCTCA AAATCTGA