afap1 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_691682.6	XP_696774.4	actin filament-associated protein 1 isoform X2
XM_005156228.3	XP_005156285.1	actin filament-associated protein 1 isoform X3
XM_005156227.4	XP_005156284.1	actin filament-associated protein 1 isoform X1
XM_005156227.3	XP_005156284.1	actin filament-associated protein 1 isoform X1
XM_005156228.4	XP_005156285.1	actin filament-associated protein 1 isoform X3
XM_021472533.1	XP_021328208.1	actin filament-associated protein 1 isoform X1
XM_021472535.1	XP_021328210.1	actin filament-associated protein 1 isoform X3
XM_021472534.1	XP_021328209.1	actin filament-associated protein 1 isoform X2
XM_691682.7	XP_696774.4	actin filament-associated protein 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17897	XM_005156227.4 Latest version!	Danio rerio actin filament associated protein 1 (afap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17898	XM_691682.7 Latest version!	Danio rerio actin filament associated protein 1 (afap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17899	XM_005156228.4 Latest version!	Danio rerio actin filament associated protein 1 (afap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa145364	XM_021472533.1 Latest version!	Danio rerio actin filament associated protein 1 (afap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa145365	XM_021472534.1 Latest version!	Danio rerio actin filament associated protein 1 (afap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa145366	XM_021472535.1 Latest version!	Danio rerio actin filament associated protein 1 (afap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa17897	XM_005156227.3	Danio rerio actin filament associated protein 1 (afap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17899	XM_005156228.3	Danio rerio actin filament associated protein 1 (afap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17898	XM_691682.6	Danio rerio actin filament associated protein 1 (afap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

afap1 ( XM_005156227.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156284.1 Danio rerio actin filament associated protein 1 (afap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17897
Clone ID Related Accession (Same CDS sequence)	XM_005156227.4 , XM_005156227.3
Accession Version	XM_005156227.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	actin filament-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156227.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGATTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAAAAG 
AAGCATGAAC TGAAGATCGT TCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GCGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATCCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATACCAGA TGCACACTAC 
TCCTCCCCAG CTTCAGGGGG CCGCCGCTGG AAAGACGAAT CTCATTATGA AAATACAGAT 
CTTTATGAAA ACATGCCCTC CCCCAAACTA GCAGCTCGCT ACTCTCCCAA GTCCACCCGC 
ACATCATCTT CCTCGGGGCA TTACAGCACA CCAAACTCCA CCAAATCTCT GTCTTCTTCC 
TCTGCTGCTA ATACTAACAC TAACCAGATG AAAGGGAAGA AAGGCTTGAT CACCAATGGT 
TTCGGTGCAA AGAAGTTGGA CAAGAATCAA CCCAAAAAGG CCAATGGCAT TAGCAGCACT 
CTGCCAGTGA AGCGTAACAA CTCCAGTGTA GATCAGTATA AGTACGGGAA AAACAGAGTG 
GAGGCAGATG CCAAGAAACT CCAAGCCAAA GAGGAGGAGC TGATGAGGAA GAAACAGGAA 
ATCCGAAACC GCCTGACCCA GCTGAAAAAA GACAGAAAGG ACCTCCGCAC TGCTTTAGAG 
AACAACACAG CTAAGCGTTC CCAGGCATCT TTGACAGAGA GGCTTAAGAA AGTGGAGGAT 
GAGTGCAAAC TGAAAGAGGA AGAGCGAGTC AGTCTGGAGC TGGAGTTGAC GGAGGTCAAG 
GAGAGTCTGA AGAAAGCTCT GAATGGTGGT GTCACCCTGG GCCTGACCAT TGAACCCAAA 
ACAGGCAGCT CCAGCCCACA GTCTCCAGTA TTAATGAGGC GAACGGTGGA CAACTCGCCC 
ATTTCCAGCT GTAACACCAG TGACACGGAG ACCTGCTCTC TGCCGGTCAA CAGTGCGTCT 
CTGCTGCGCC GCCAGACACA ACAGAAAGCC TCACCTGTGC GAGGTCACGT CCTCAGAAAA 
GCTAAGGAAT GGGAGATGAA ATCTGGCACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156284.1
CDS	388..2820
Translation	MRSDPILFASRAMEELLAELRVFLELLDREYLTAGVREKKQQILNILHRVLATREPSCKTEIHTSLPAPPQMPLPEIPHPWMPPDNGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDQTPQMELLLSGCSITHIPKDGKKKKHELKIVHQGADALVLAVQSKEQAEQWLKVMKEVCSNGNGVVDCDGAGSGSTVHKAELEKKLSCDRPSSDGEPCHENGISDGKDPAKGKKNSKSEQKGTVGRVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCQLKDNQLFLHKDRADLKTHMASLPLRGCEVIPGLDSKHPFAFRLLRNGQEVAVLEASSSESMGRWLGVLLAETGSTTDPAALHYDYIDVETTANVIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGVALHYDDVPCINGTIPDAHYSSPASGGRRWKDESHYENTDLYENMPSPKLAARYSPKSTRTSSSSGHYSTPNSTKSLSSSSAANTNTNQMKGKKGLITNGFGAKKLDKNQPKKANGISSTLPVKRNNSSVDQYKYGKNRVEADAKKLQAKEEELMRKKQEIRNRLTQLKKDRKDLRTALENNTAKRSQASLTERLKKVEDECKLKEEERVSLELELTEVKESLKKALNGGVTLGLTIEPKTGSSSPQSPVLMRRTVDNSPISSCNTSDTETCSLPVNSASLLRRQTQQKASPVRGHVLRKAKEWEMKSGT

Target ORF information:

RefSeq Version	XM_005156227.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin filament associated protein 1 (afap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156227.4

ORF Insert Sequence:

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGATTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAAAAG 
AAGCATGAAC TGAAGATCGT TCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GCGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATCCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATACCAGA TGCACACTAC 
TCCTCCCCAG CTTCAGGGGG CCGCCGCTGG AAAGACGAAT CTCATTATGA AAATACAGAT 
CTTTATGAAA ACATGCCCTC CCCCAAACTA GCAGCTCGCT ACTCTCCCAA GTCCACCCGC 
ACATCATCTT CCTCGGGGCA TTACAGCACA CCAAACTCCA CCAAATCTCT GTCTTCTTCC 
TCTGCTGCTA ATACTAACAC TAACCAGATG AAAGGGAAGA AAGGCTTGAT CACCAATGGT 
TTCGGTGCAA AGAAGTTGGA CAAGAATCAA CCCAAAAAGG CCAATGGCAT TAGCAGCACT 
CTGCCAGTGA AGCGTAACAA CTCCAGTGTA GATCAGTATA AGTACGGGAA AAACAGAGTG 
GAGGCAGATG CCAAGAAACT CCAAGCCAAA GAGGAGGAGC TGATGAGGAA GAAACAGGAA 
ATCCGAAACC GCCTGACCCA GCTGAAAAAA GACAGAAAGG ACCTCCGCAC TGCTTTAGAG 
AACAACACAG CTAAGCGTTC CCAGGCATCT TTGACAGAGA GGCTTAAGAA AGTGGAGGAT 
GAGTGCAAAC TGAAAGAGGA AGAGCGAGTC AGTCTGGAGC TGGAGTTGAC GGAGGTCAAG 
GAGAGTCTGA AGAAAGCTCT GAATGGTGGT GTCACCCTGG GCCTGACCAT TGAACCCAAA 
ACAGGCAGCT CCAGCCCACA GTCTCCAGTA TTAATGAGGC GAACGGTGGA CAACTCGCCC 
ATTTCCAGCT GTAACACCAG TGACACGGAG ACCTGCTCTC TGCCGGTCAA CAGTGCGTCT 
CTGCTGCGCC GCCAGACACA ACAGAAAGCC TCACCTGTGC GAGGTCACGT CCTCAGAAAA 
GCTAAGGAAT GGGAGATGAA ATCTGGCACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_005156227.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156284.1 Danio rerio actin filament associated protein 1 (afap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17897
Clone ID Related Accession (Same CDS sequence)	XM_005156227.4 , XM_005156227.3
Accession Version	XM_005156227.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	actin filament-associated protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156227.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGATTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAAAAG 
AAGCATGAAC TGAAGATCGT TCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GCGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATCCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATACCAGA TGCACACTAC 
TCCTCCCCAG CTTCAGGGGG CCGCCGCTGG AAAGACGAAT CTCATTATGA AAATACAGAT 
CTTTATGAAA ACATGCCCTC CCCCAAACTA GCAGCTCGCT ACTCTCCCAA GTCCACCCGC 
ACATCATCTT CCTCGGGGCA TTACAGCACA CCAAACTCCA CCAAATCTCT GTCTTCTTCC 
TCTGCTGCTA ATACTAACAC TAACCAGATG AAAGGGAAGA AAGGCTTGAT CACCAATGGT 
TTCGGTGCAA AGAAGTTGGA CAAGAATCAA CCCAAAAAGG CCAATGGCAT TAGCAGCACT 
CTGCCAGTGA AGCGTAACAA CTCCAGTGTA GATCAGTATA AGTACGGGAA AAACAGAGTG 
GAGGCAGATG CCAAGAAACT CCAAGCCAAA GAGGAGGAGC TGATGAGGAA GAAACAGGAA 
ATCCGAAACC GCCTGACCCA GCTGAAAAAA GACAGAAAGG ACCTCCGCAC TGCTTTAGAG 
AACAACACAG CTAAGCGTTC CCAGGCATCT TTGACAGAGA GGCTTAAGAA AGTGGAGGAT 
GAGTGCAAAC TGAAAGAGGA AGAGCGAGTC AGTCTGGAGC TGGAGTTGAC GGAGGTCAAG 
GAGAGTCTGA AGAAAGCTCT GAATGGTGGT GTCACCCTGG GCCTGACCAT TGAACCCAAA 
ACAGGCAGCT CCAGCCCACA GTCTCCAGTA TTAATGAGGC GAACGGTGGA CAACTCGCCC 
ATTTCCAGCT GTAACACCAG TGACACGGAG ACCTGCTCTC TGCCGGTCAA CAGTGCGTCT 
CTGCTGCGCC GCCAGACACA ACAGAAAGCC TCACCTGTGC GAGGTCACGT CCTCAGAAAA 
GCTAAGGAAT GGGAGATGAA ATCTGGCACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156284.1
CDS	451..2883
Translation	MRSDPILFASRAMEELLAELRVFLELLDREYLTAGVREKKQQILNILHRVLATREPSCKTEIHTSLPAPPQMPLPEIPHPWMPPDNGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDQTPQMELLLSGCSITHIPKDGKKKKHELKIVHQGADALVLAVQSKEQAEQWLKVMKEVCSNGNGVVDCDGAGSGSTVHKAELEKKLSCDRPSSDGEPCHENGISDGKDPAKGKKNSKSEQKGTVGRVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCQLKDNQLFLHKDRADLKTHMASLPLRGCEVIPGLDSKHPFAFRLLRNGQEVAVLEASSSESMGRWLGVLLAETGSTTDPAALHYDYIDVETTANVIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGVALHYDDVPCINGTIPDAHYSSPASGGRRWKDESHYENTDLYENMPSPKLAARYSPKSTRTSSSSGHYSTPNSTKSLSSSSAANTNTNQMKGKKGLITNGFGAKKLDKNQPKKANGISSTLPVKRNNSSVDQYKYGKNRVEADAKKLQAKEEELMRKKQEIRNRLTQLKKDRKDLRTALENNTAKRSQASLTERLKKVEDECKLKEEERVSLELELTEVKESLKKALNGGVTLGLTIEPKTGSSSPQSPVLMRRTVDNSPISSCNTSDTETCSLPVNSASLLRRQTQQKASPVRGHVLRKAKEWEMKSGT

Target ORF information:

RefSeq Version	XM_005156227.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio actin filament associated protein 1 (afap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156227.3

ORF Insert Sequence:

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGATTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAAAAG 
AAGCATGAAC TGAAGATCGT TCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GCGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATCCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATACCAGA TGCACACTAC 
TCCTCCCCAG CTTCAGGGGG CCGCCGCTGG AAAGACGAAT CTCATTATGA AAATACAGAT 
CTTTATGAAA ACATGCCCTC CCCCAAACTA GCAGCTCGCT ACTCTCCCAA GTCCACCCGC 
ACATCATCTT CCTCGGGGCA TTACAGCACA CCAAACTCCA CCAAATCTCT GTCTTCTTCC 
TCTGCTGCTA ATACTAACAC TAACCAGATG AAAGGGAAGA AAGGCTTGAT CACCAATGGT 
TTCGGTGCAA AGAAGTTGGA CAAGAATCAA CCCAAAAAGG CCAATGGCAT TAGCAGCACT 
CTGCCAGTGA AGCGTAACAA CTCCAGTGTA GATCAGTATA AGTACGGGAA AAACAGAGTG 
GAGGCAGATG CCAAGAAACT CCAAGCCAAA GAGGAGGAGC TGATGAGGAA GAAACAGGAA 
ATCCGAAACC GCCTGACCCA GCTGAAAAAA GACAGAAAGG ACCTCCGCAC TGCTTTAGAG 
AACAACACAG CTAAGCGTTC CCAGGCATCT TTGACAGAGA GGCTTAAGAA AGTGGAGGAT 
GAGTGCAAAC TGAAAGAGGA AGAGCGAGTC AGTCTGGAGC TGGAGTTGAC GGAGGTCAAG 
GAGAGTCTGA AGAAAGCTCT GAATGGTGGT GTCACCCTGG GCCTGACCAT TGAACCCAAA 
ACAGGCAGCT CCAGCCCACA GTCTCCAGTA TTAATGAGGC GAACGGTGGA CAACTCGCCC 
ATTTCCAGCT GTAACACCAG TGACACGGAG ACCTGCTCTC TGCCGGTCAA CAGTGCGTCT 
CTGCTGCGCC GCCAGACACA ACAGAAAGCC TCACCTGTGC GAGGTCACGT CCTCAGAAAA 
GCTAAGGAAT GGGAGATGAA ATCTGGCACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_691682.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_696774.4 Danio rerio actin filament associated protein 1 (afap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17898
Clone ID Related Accession (Same CDS sequence)	XM_691682.6 , XM_691682.7
Accession Version	XM_691682.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	actin filament-associated protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_691682.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAA GCAACGAAGA AGCGATGGAG GAGTTGTTGG CAGAGTTACG AGTGTTTCTG 
GAGTTGTTGG ACAGAGAGTA TTTGACTGCT GGAGTGAGAG AGAAGAAACA GCAAATATTA 
AACATCCTGC ACAGAGTTCT GGCCACTAGA GAACCCTCAT GTAAGACAGA GATACACACA 
TCTCTCCCGG CCCCTCCCCA GATGCCCCTA CCAGAGATTC CTCACCCCTG GATGCCTCCA 
GACAATGGGC CTCCTCCTTT ACCGAGCTCC TCTCTTCCTG AGGGTTATTA TGAGGAAGCG 
GTTCCACTGG GTCCTGGGAA AGCACCAGAG TACATCACCT CTAATTATGA CTCGGACGCC 
ATGAGCAGTT CGTATGAGTC ATATGATGAG GAAGAGGAGG ATGGGAAGGG TCAGAAGATG 
CGTCACCAGT GGCCATCTGA GGAGGCCTCC ATGGATTTGG TAAAAGATGC ACGTATCTGC 
GCATTCCTGT TGCGAAAGAA ACGCTTTGGT CAGTGGACCA AACTTCTATG TGTCATAAAG 
GACAACAAAC TGTTGTGCTA CAAGTCCTCT AAAGACCAGA CTCCACAGAT GGAGCTCCTT 
CTGTCCGGCT GCAGTATCAC ACACATCCCC AAAGATGGCA AGAAAAAGAA GCATGAACTG 
AAGATCGTTC ATCAGGGAGC TGATGCTCTA GTGCTTGCTG TACAGAGCAA AGAACAGGCC 
GAACAATGGC TAAAGGTGAT GAAAGAAGTC TGCTCGAATG GAAATGGAGT GGTGGATTGC 
GACGGAGCTG GCTCTGGATC CACTGTTCAC AAGGCAGAGC TGGAAAAGAA GCTGTCCTGC 
GATCGGCCCA GTTCAGATGG AGAGCCCTGC CATGAAAACG GCATCTCAGA TGGCAAAGAT 
CCAGCTAAAG GCAAGAAGAA CTCTAAATCA GAGCAGAAAG GCACTGTGGG ACGTGTGACT 
GGAAAGAAAA TCACAAAAAT CATCAGCCTA GGCAAGAAGA AACCATCAAC AGATGAACAG 
ACCTCATCAG CGGAGGAAGA TGTCCCAACA TGTGGCTATC TTAACGTGCT GTCCAACAAC 
CGCTGGAGAG AGCGCTGGTG CCAGCTAAAA GACAACCAGC TGTTTCTGCA CAAAGATCGA 
GCCGATCTGA AGACGCACAT GGCCTCTCTG CCGCTGCGGG GCTGTGAGGT CATTCCAGGC 
CTGGACTCCA AACATCCCTT CGCCTTCCGT CTACTGCGCA ATGGACAGGA GGTGGCTGTT 
CTGGAGGCCT CCTCCTCTGA GAGTATGGGA AGGTGGTTGG GGGTCTTGCT GGCTGAGACG 
GGCTCCACAA CAGATCCCGC TGCACTCCAC TACGATTACA TTGACGTAGA GACCACTGCT 
AACGTCATTC AGCTGGCAAA GCAGTCATTT TGTTTCACCA GTAAGCGTGC AGTTTCTCCT 
AACCCATACC TGGATAATCC GGTCAATGGT TATGCCTGTC CCACTGGGGT CGCTCTGCAT 
TATGACGATG TGCCCTGCAT CAATGGAACG ATACCAGATG CACACTACTC CTCCCCAGCT 
TCAGGGGGCC GCCGCTGGAA AGACGAATCT CATTATGAAA ATACAGATCT TTATGAAAAC 
ATGCCCTCCC CCAAACTAGC AGCTCGCTAC TCTCCCAAGT CCACCCGCAC ATCATCTTCC 
TCGGGGCATT ACAGCACACC AAACTCCACC AAATCTCTGT CTTCTTCCTC TGCTGCTAAT 
ACTAACACTA ACCAGATGAA AGGGAAGAAA GGCTTGATCA CCAATGGTTT CGGTGCAAAG 
AAGTTGGACA AGAATCAACC CAAAAAGGCC AATGGCATTA GCAGCACTCT GCCAGTGAAG 
CGTAACAACT CCAGTGTAGA TCAGTATAAG TACGGGAAAA ACAGAGTGGA GGCAGATGCC 
AAGAAACTCC AAGCCAAAGA GGAGGAGCTG ATGAGGAAGA AACAGGAAAT CCGAAACCGC 
CTGACCCAGC TGAAAAAAGA CAGAAAGGAC CTCCGCACTG CTTTAGAGAA CAACACAGCT 
AAGCGTTCCC AGGCATCTTT GACAGAGAGG CTTAAGAAAG TGGAGGATGA GTGCAAACTG 
AAAGAGGAAG AGCGAGTCAG TCTGGAGCTG GAGTTGACGG AGGTCAAGGA GAGTCTGAAG 
AAAGCTCTGA ATGGTGGTGT CACCCTGGGC CTGACCATTG AACCCAAAAC AGGCAGCTCC 
AGCCCACAGT CTCCAGTATT AATGAGGCGA ACGGTGGACA ACTCGCCCAT TTCCAGCTGT 
AACACCAGTG ACACGGAGAC CTGCTCTCTG CCGGTCAACA GTGCGTCTCT GCTGCGCCGC 
CAGACACAAC AGAAAGCCTC ACCTGTGCGA GGTCACGTCC TCAGAAAAGC TAAGGAATGG 
GAGATGAAAT CTGGCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_696774.4
CDS	120..2540
Translation	MTESNEEAMEELLAELRVFLELLDREYLTAGVREKKQQILNILHRVLATREPSCKTEIHTSLPAPPQMPLPEIPHPWMPPDNGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDQTPQMELLLSGCSITHIPKDGKKKKHELKIVHQGADALVLAVQSKEQAEQWLKVMKEVCSNGNGVVDCDGAGSGSTVHKAELEKKLSCDRPSSDGEPCHENGISDGKDPAKGKKNSKSEQKGTVGRVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCQLKDNQLFLHKDRADLKTHMASLPLRGCEVIPGLDSKHPFAFRLLRNGQEVAVLEASSSESMGRWLGVLLAETGSTTDPAALHYDYIDVETTANVIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGVALHYDDVPCINGTIPDAHYSSPASGGRRWKDESHYENTDLYENMPSPKLAARYSPKSTRTSSSSGHYSTPNSTKSLSSSSAANTNTNQMKGKKGLITNGFGAKKLDKNQPKKANGISSTLPVKRNNSSVDQYKYGKNRVEADAKKLQAKEEELMRKKQEIRNRLTQLKKDRKDLRTALENNTAKRSQASLTERLKKVEDECKLKEEERVSLELELTEVKESLKKALNGGVTLGLTIEPKTGSSSPQSPVLMRRTVDNSPISSCNTSDTETCSLPVNSASLLRRQTQQKASPVRGHVLRKAKEWEMKSGT

Target ORF information:

RefSeq Version	XM_691682.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio actin filament associated protein 1 (afap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_691682.6

ORF Insert Sequence:

ATGACGGAAA GCAACGAAGA AGCGATGGAG GAGTTGTTGG CAGAGTTACG AGTGTTTCTG 
GAGTTGTTGG ACAGAGAGTA TTTGACTGCT GGAGTGAGAG AGAAGAAACA GCAAATATTA 
AACATCCTGC ACAGAGTTCT GGCCACTAGA GAACCCTCAT GTAAGACAGA GATACACACA 
TCTCTCCCGG CCCCTCCCCA GATGCCCCTA CCAGAGATTC CTCACCCCTG GATGCCTCCA 
GACAATGGGC CTCCTCCTTT ACCGAGCTCC TCTCTTCCTG AGGGTTATTA TGAGGAAGCG 
GTTCCACTGG GTCCTGGGAA AGCACCAGAG TACATCACCT CTAATTATGA CTCGGACGCC 
ATGAGCAGTT CGTATGAGTC ATATGATGAG GAAGAGGAGG ATGGGAAGGG TCAGAAGATG 
CGTCACCAGT GGCCATCTGA GGAGGCCTCC ATGGATTTGG TAAAAGATGC ACGTATCTGC 
GCATTCCTGT TGCGAAAGAA ACGCTTTGGT CAGTGGACCA AACTTCTATG TGTCATAAAG 
GACAACAAAC TGTTGTGCTA CAAGTCCTCT AAAGACCAGA CTCCACAGAT GGAGCTCCTT 
CTGTCCGGCT GCAGTATCAC ACACATCCCC AAAGATGGCA AGAAAAAGAA GCATGAACTG 
AAGATCGTTC ATCAGGGAGC TGATGCTCTA GTGCTTGCTG TACAGAGCAA AGAACAGGCC 
GAACAATGGC TAAAGGTGAT GAAAGAAGTC TGCTCGAATG GAAATGGAGT GGTGGATTGC 
GACGGAGCTG GCTCTGGATC CACTGTTCAC AAGGCAGAGC TGGAAAAGAA GCTGTCCTGC 
GATCGGCCCA GTTCAGATGG AGAGCCCTGC CATGAAAACG GCATCTCAGA TGGCAAAGAT 
CCAGCTAAAG GCAAGAAGAA CTCTAAATCA GAGCAGAAAG GCACTGTGGG ACGTGTGACT 
GGAAAGAAAA TCACAAAAAT CATCAGCCTA GGCAAGAAGA AACCATCAAC AGATGAACAG 
ACCTCATCAG CGGAGGAAGA TGTCCCAACA TGTGGCTATC TTAACGTGCT GTCCAACAAC 
CGCTGGAGAG AGCGCTGGTG CCAGCTAAAA GACAACCAGC TGTTTCTGCA CAAAGATCGA 
GCCGATCTGA AGACGCACAT GGCCTCTCTG CCGCTGCGGG GCTGTGAGGT CATTCCAGGC 
CTGGACTCCA AACATCCCTT CGCCTTCCGT CTACTGCGCA ATGGACAGGA GGTGGCTGTT 
CTGGAGGCCT CCTCCTCTGA GAGTATGGGA AGGTGGTTGG GGGTCTTGCT GGCTGAGACG 
GGCTCCACAA CAGATCCCGC TGCACTCCAC TACGATTACA TTGACGTAGA GACCACTGCT 
AACGTCATTC AGCTGGCAAA GCAGTCATTT TGTTTCACCA GTAAGCGTGC AGTTTCTCCT 
AACCCATACC TGGATAATCC GGTCAATGGT TATGCCTGTC CCACTGGGGT CGCTCTGCAT 
TATGACGATG TGCCCTGCAT CAATGGAACG ATACCAGATG CACACTACTC CTCCCCAGCT 
TCAGGGGGCC GCCGCTGGAA AGACGAATCT CATTATGAAA ATACAGATCT TTATGAAAAC 
ATGCCCTCCC CCAAACTAGC AGCTCGCTAC TCTCCCAAGT CCACCCGCAC ATCATCTTCC 
TCGGGGCATT ACAGCACACC AAACTCCACC AAATCTCTGT CTTCTTCCTC TGCTGCTAAT 
ACTAACACTA ACCAGATGAA AGGGAAGAAA GGCTTGATCA CCAATGGTTT CGGTGCAAAG 
AAGTTGGACA AGAATCAACC CAAAAAGGCC AATGGCATTA GCAGCACTCT GCCAGTGAAG 
CGTAACAACT CCAGTGTAGA TCAGTATAAG TACGGGAAAA ACAGAGTGGA GGCAGATGCC 
AAGAAACTCC AAGCCAAAGA GGAGGAGCTG ATGAGGAAGA AACAGGAAAT CCGAAACCGC 
CTGACCCAGC TGAAAAAAGA CAGAAAGGAC CTCCGCACTG CTTTAGAGAA CAACACAGCT 
AAGCGTTCCC AGGCATCTTT GACAGAGAGG CTTAAGAAAG TGGAGGATGA GTGCAAACTG 
AAAGAGGAAG AGCGAGTCAG TCTGGAGCTG GAGTTGACGG AGGTCAAGGA GAGTCTGAAG 
AAAGCTCTGA ATGGTGGTGT CACCCTGGGC CTGACCATTG AACCCAAAAC AGGCAGCTCC 
AGCCCACAGT CTCCAGTATT AATGAGGCGA ACGGTGGACA ACTCGCCCAT TTCCAGCTGT 
AACACCAGTG ACACGGAGAC CTGCTCTCTG CCGGTCAACA GTGCGTCTCT GCTGCGCCGC 
CAGACACAAC AGAAAGCCTC ACCTGTGCGA GGTCACGTCC TCAGAAAAGC TAAGGAATGG 
GAGATGAAAT CTGGCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_691682.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_696774.4 Danio rerio actin filament associated protein 1 (afap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17898
Clone ID Related Accession (Same CDS sequence)	XM_691682.6 , XM_691682.7
Accession Version	XM_691682.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	actin filament-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_691682.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAA GCAACGAAGA AGCGATGGAG GAGTTGTTGG CAGAGTTACG AGTGTTTCTG 
GAGTTGTTGG ACAGAGAGTA TTTGACTGCT GGAGTGAGAG AGAAGAAACA GCAAATATTA 
AACATCCTGC ACAGAGTTCT GGCCACTAGA GAACCCTCAT GTAAGACAGA GATACACACA 
TCTCTCCCGG CCCCTCCCCA GATGCCCCTA CCAGAGATTC CTCACCCCTG GATGCCTCCA 
GACAATGGGC CTCCTCCTTT ACCGAGCTCC TCTCTTCCTG AGGGTTATTA TGAGGAAGCG 
GTTCCACTGG GTCCTGGGAA AGCACCAGAG TACATCACCT CTAATTATGA CTCGGACGCC 
ATGAGCAGTT CGTATGAGTC ATATGATGAG GAAGAGGAGG ATGGGAAGGG TCAGAAGATG 
CGTCACCAGT GGCCATCTGA GGAGGCCTCC ATGGATTTGG TAAAAGATGC ACGTATCTGC 
GCATTCCTGT TGCGAAAGAA ACGCTTTGGT CAGTGGACCA AACTTCTATG TGTCATAAAG 
GACAACAAAC TGTTGTGCTA CAAGTCCTCT AAAGACCAGA CTCCACAGAT GGAGCTCCTT 
CTGTCCGGCT GCAGTATCAC ACACATCCCC AAAGATGGCA AGAAAAAGAA GCATGAACTG 
AAGATCGTTC ATCAGGGAGC TGATGCTCTA GTGCTTGCTG TACAGAGCAA AGAACAGGCC 
GAACAATGGC TAAAGGTGAT GAAAGAAGTC TGCTCGAATG GAAATGGAGT GGTGGATTGC 
GACGGAGCTG GCTCTGGATC CACTGTTCAC AAGGCAGAGC TGGAAAAGAA GCTGTCCTGC 
GATCGGCCCA GTTCAGATGG AGAGCCCTGC CATGAAAACG GCATCTCAGA TGGCAAAGAT 
CCAGCTAAAG GCAAGAAGAA CTCTAAATCA GAGCAGAAAG GCACTGTGGG ACGTGTGACT 
GGAAAGAAAA TCACAAAAAT CATCAGCCTA GGCAAGAAGA AACCATCAAC AGATGAACAG 
ACCTCATCAG CGGAGGAAGA TGTCCCAACA TGTGGCTATC TTAACGTGCT GTCCAACAAC 
CGCTGGAGAG AGCGCTGGTG CCAGCTAAAA GACAACCAGC TGTTTCTGCA CAAAGATCGA 
GCCGATCTGA AGACGCACAT GGCCTCTCTG CCGCTGCGGG GCTGTGAGGT CATTCCAGGC 
CTGGACTCCA AACATCCCTT CGCCTTCCGT CTACTGCGCA ATGGACAGGA GGTGGCTGTT 
CTGGAGGCCT CCTCCTCTGA GAGTATGGGA AGGTGGTTGG GGGTCTTGCT GGCTGAGACG 
GGCTCCACAA CAGATCCCGC TGCACTCCAC TACGATTACA TTGACGTAGA GACCACTGCT 
AACGTCATTC AGCTGGCAAA GCAGTCATTT TGTTTCACCA GTAAGCGTGC AGTTTCTCCT 
AACCCATACC TGGATAATCC GGTCAATGGT TATGCCTGTC CCACTGGGGT CGCTCTGCAT 
TATGACGATG TGCCCTGCAT CAATGGAACG ATACCAGATG CACACTACTC CTCCCCAGCT 
TCAGGGGGCC GCCGCTGGAA AGACGAATCT CATTATGAAA ATACAGATCT TTATGAAAAC 
ATGCCCTCCC CCAAACTAGC AGCTCGCTAC TCTCCCAAGT CCACCCGCAC ATCATCTTCC 
TCGGGGCATT ACAGCACACC AAACTCCACC AAATCTCTGT CTTCTTCCTC TGCTGCTAAT 
ACTAACACTA ACCAGATGAA AGGGAAGAAA GGCTTGATCA CCAATGGTTT CGGTGCAAAG 
AAGTTGGACA AGAATCAACC CAAAAAGGCC AATGGCATTA GCAGCACTCT GCCAGTGAAG 
CGTAACAACT CCAGTGTAGA TCAGTATAAG TACGGGAAAA ACAGAGTGGA GGCAGATGCC 
AAGAAACTCC AAGCCAAAGA GGAGGAGCTG ATGAGGAAGA AACAGGAAAT CCGAAACCGC 
CTGACCCAGC TGAAAAAAGA CAGAAAGGAC CTCCGCACTG CTTTAGAGAA CAACACAGCT 
AAGCGTTCCC AGGCATCTTT GACAGAGAGG CTTAAGAAAG TGGAGGATGA GTGCAAACTG 
AAAGAGGAAG AGCGAGTCAG TCTGGAGCTG GAGTTGACGG AGGTCAAGGA GAGTCTGAAG 
AAAGCTCTGA ATGGTGGTGT CACCCTGGGC CTGACCATTG AACCCAAAAC AGGCAGCTCC 
AGCCCACAGT CTCCAGTATT AATGAGGCGA ACGGTGGACA ACTCGCCCAT TTCCAGCTGT 
AACACCAGTG ACACGGAGAC CTGCTCTCTG CCGGTCAACA GTGCGTCTCT GCTGCGCCGC 
CAGACACAAC AGAAAGCCTC ACCTGTGCGA GGTCACGTCC TCAGAAAAGC TAAGGAATGG 
GAGATGAAAT CTGGCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_696774.4
CDS	130..2550
Translation	MTESNEEAMEELLAELRVFLELLDREYLTAGVREKKQQILNILHRVLATREPSCKTEIHTSLPAPPQMPLPEIPHPWMPPDNGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDQTPQMELLLSGCSITHIPKDGKKKKHELKIVHQGADALVLAVQSKEQAEQWLKVMKEVCSNGNGVVDCDGAGSGSTVHKAELEKKLSCDRPSSDGEPCHENGISDGKDPAKGKKNSKSEQKGTVGRVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCQLKDNQLFLHKDRADLKTHMASLPLRGCEVIPGLDSKHPFAFRLLRNGQEVAVLEASSSESMGRWLGVLLAETGSTTDPAALHYDYIDVETTANVIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGVALHYDDVPCINGTIPDAHYSSPASGGRRWKDESHYENTDLYENMPSPKLAARYSPKSTRTSSSSGHYSTPNSTKSLSSSSAANTNTNQMKGKKGLITNGFGAKKLDKNQPKKANGISSTLPVKRNNSSVDQYKYGKNRVEADAKKLQAKEEELMRKKQEIRNRLTQLKKDRKDLRTALENNTAKRSQASLTERLKKVEDECKLKEEERVSLELELTEVKESLKKALNGGVTLGLTIEPKTGSSSPQSPVLMRRTVDNSPISSCNTSDTETCSLPVNSASLLRRQTQQKASPVRGHVLRKAKEWEMKSGT

Target ORF information:

RefSeq Version	XM_691682.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin filament associated protein 1 (afap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_691682.7

ORF Insert Sequence:

ATGACGGAAA GCAACGAAGA AGCGATGGAG GAGTTGTTGG CAGAGTTACG AGTGTTTCTG 
GAGTTGTTGG ACAGAGAGTA TTTGACTGCT GGAGTGAGAG AGAAGAAACA GCAAATATTA 
AACATCCTGC ACAGAGTTCT GGCCACTAGA GAACCCTCAT GTAAGACAGA GATACACACA 
TCTCTCCCGG CCCCTCCCCA GATGCCCCTA CCAGAGATTC CTCACCCCTG GATGCCTCCA 
GACAATGGGC CTCCTCCTTT ACCGAGCTCC TCTCTTCCTG AGGGTTATTA TGAGGAAGCG 
GTTCCACTGG GTCCTGGGAA AGCACCAGAG TACATCACCT CTAATTATGA CTCGGACGCC 
ATGAGCAGTT CGTATGAGTC ATATGATGAG GAAGAGGAGG ATGGGAAGGG TCAGAAGATG 
CGTCACCAGT GGCCATCTGA GGAGGCCTCC ATGGATTTGG TAAAAGATGC ACGTATCTGC 
GCATTCCTGT TGCGAAAGAA ACGCTTTGGT CAGTGGACCA AACTTCTATG TGTCATAAAG 
GACAACAAAC TGTTGTGCTA CAAGTCCTCT AAAGACCAGA CTCCACAGAT GGAGCTCCTT 
CTGTCCGGCT GCAGTATCAC ACACATCCCC AAAGATGGCA AGAAAAAGAA GCATGAACTG 
AAGATCGTTC ATCAGGGAGC TGATGCTCTA GTGCTTGCTG TACAGAGCAA AGAACAGGCC 
GAACAATGGC TAAAGGTGAT GAAAGAAGTC TGCTCGAATG GAAATGGAGT GGTGGATTGC 
GACGGAGCTG GCTCTGGATC CACTGTTCAC AAGGCAGAGC TGGAAAAGAA GCTGTCCTGC 
GATCGGCCCA GTTCAGATGG AGAGCCCTGC CATGAAAACG GCATCTCAGA TGGCAAAGAT 
CCAGCTAAAG GCAAGAAGAA CTCTAAATCA GAGCAGAAAG GCACTGTGGG ACGTGTGACT 
GGAAAGAAAA TCACAAAAAT CATCAGCCTA GGCAAGAAGA AACCATCAAC AGATGAACAG 
ACCTCATCAG CGGAGGAAGA TGTCCCAACA TGTGGCTATC TTAACGTGCT GTCCAACAAC 
CGCTGGAGAG AGCGCTGGTG CCAGCTAAAA GACAACCAGC TGTTTCTGCA CAAAGATCGA 
GCCGATCTGA AGACGCACAT GGCCTCTCTG CCGCTGCGGG GCTGTGAGGT CATTCCAGGC 
CTGGACTCCA AACATCCCTT CGCCTTCCGT CTACTGCGCA ATGGACAGGA GGTGGCTGTT 
CTGGAGGCCT CCTCCTCTGA GAGTATGGGA AGGTGGTTGG GGGTCTTGCT GGCTGAGACG 
GGCTCCACAA CAGATCCCGC TGCACTCCAC TACGATTACA TTGACGTAGA GACCACTGCT 
AACGTCATTC AGCTGGCAAA GCAGTCATTT TGTTTCACCA GTAAGCGTGC AGTTTCTCCT 
AACCCATACC TGGATAATCC GGTCAATGGT TATGCCTGTC CCACTGGGGT CGCTCTGCAT 
TATGACGATG TGCCCTGCAT CAATGGAACG ATACCAGATG CACACTACTC CTCCCCAGCT 
TCAGGGGGCC GCCGCTGGAA AGACGAATCT CATTATGAAA ATACAGATCT TTATGAAAAC 
ATGCCCTCCC CCAAACTAGC AGCTCGCTAC TCTCCCAAGT CCACCCGCAC ATCATCTTCC 
TCGGGGCATT ACAGCACACC AAACTCCACC AAATCTCTGT CTTCTTCCTC TGCTGCTAAT 
ACTAACACTA ACCAGATGAA AGGGAAGAAA GGCTTGATCA CCAATGGTTT CGGTGCAAAG 
AAGTTGGACA AGAATCAACC CAAAAAGGCC AATGGCATTA GCAGCACTCT GCCAGTGAAG 
CGTAACAACT CCAGTGTAGA TCAGTATAAG TACGGGAAAA ACAGAGTGGA GGCAGATGCC 
AAGAAACTCC AAGCCAAAGA GGAGGAGCTG ATGAGGAAGA AACAGGAAAT CCGAAACCGC 
CTGACCCAGC TGAAAAAAGA CAGAAAGGAC CTCCGCACTG CTTTAGAGAA CAACACAGCT 
AAGCGTTCCC AGGCATCTTT GACAGAGAGG CTTAAGAAAG TGGAGGATGA GTGCAAACTG 
AAAGAGGAAG AGCGAGTCAG TCTGGAGCTG GAGTTGACGG AGGTCAAGGA GAGTCTGAAG 
AAAGCTCTGA ATGGTGGTGT CACCCTGGGC CTGACCATTG AACCCAAAAC AGGCAGCTCC 
AGCCCACAGT CTCCAGTATT AATGAGGCGA ACGGTGGACA ACTCGCCCAT TTCCAGCTGT 
AACACCAGTG ACACGGAGAC CTGCTCTCTG CCGGTCAACA GTGCGTCTCT GCTGCGCCGC 
CAGACACAAC AGAAAGCCTC ACCTGTGCGA GGTCACGTCC TCAGAAAAGC TAAGGAATGG 
GAGATGAAAT CTGGCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_005156228.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156285.1 Danio rerio actin filament associated protein 1 (afap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa17899
Clone ID Related Accession (Same CDS sequence)	XM_005156228.3 , XM_005156228.4
Accession Version	XM_005156228.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	actin filament-associated protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156228.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGATTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAAAAG 
AAGCATGAAC TGAAGATCGT TCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GCGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATCCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATGAAAGG GAAGAAAGGC 
TTGATCACCA ATGGTTTCGG TGCAAAGAAG TTGGACAAGA ATCAACCCAA AAAGGCCAAT 
GGCATTAGCA GCACTCTGCC AGTGAAGCGT AACAACTCCA GTGTAGATCA GTATAAGTAC 
GGGAAAAACA GAGTGGAGGC AGATGCCAAG AAACTCCAAG CCAAAGAGGA GGAGCTGATG 
AGGAAGAAAC AGGAAATCCG AAACCGCCTG ACCCAGCTGA AAAAAGACAG AAAGGACCTC 
CGCACTGCTT TAGAGAACAA CACAGCTAAG CGTTCCCAGG CATCTTTGAC AGAGAGGCTT 
AAGAAAGTGG AGGATGAGTG CAAACTGAAA GAGGAAGAGC GAGTCAGTCT GGAGCTGGAG 
TTGACGGAGG TCAAGGAGAG TCTGAAGAAA GCTCTGAATG GTGGTGTCAC CCTGGGCCTG 
ACCATTGAAC CCAAAACAGG CAGCTCCAGC CCACAGTCTC CAGTATTAAT GAGGCGAACG 
GTGGACAACT CGCCCATTTC CAGCTGTAAC ACCAGTGACA CGGAGACCTG CTCTCTGCCG 
GTCAACAGTG CGTCTCTGCT GCGCCGCCAG ACACAACAGA AAGCCTCACC TGTGCGAGGT 
CACGTCCTCA GAAAAGCTAA GGAATGGGAG ATGAAATCTG GCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156285.1
CDS	451..2658
Translation	MRSDPILFASRAMEELLAELRVFLELLDREYLTAGVREKKQQILNILHRVLATREPSCKTEIHTSLPAPPQMPLPEIPHPWMPPDNGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDQTPQMELLLSGCSITHIPKDGKKKKHELKIVHQGADALVLAVQSKEQAEQWLKVMKEVCSNGNGVVDCDGAGSGSTVHKAELEKKLSCDRPSSDGEPCHENGISDGKDPAKGKKNSKSEQKGTVGRVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCQLKDNQLFLHKDRADLKTHMASLPLRGCEVIPGLDSKHPFAFRLLRNGQEVAVLEASSSESMGRWLGVLLAETGSTTDPAALHYDYIDVETTANVIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGVALHYDDVPCINGTMKGKKGLITNGFGAKKLDKNQPKKANGISSTLPVKRNNSSVDQYKYGKNRVEADAKKLQAKEEELMRKKQEIRNRLTQLKKDRKDLRTALENNTAKRSQASLTERLKKVEDECKLKEEERVSLELELTEVKESLKKALNGGVTLGLTIEPKTGSSSPQSPVLMRRTVDNSPISSCNTSDTETCSLPVNSASLLRRQTQQKASPVRGHVLRKAKEWEMKSGT

Target ORF information:

RefSeq Version	XM_005156228.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio actin filament associated protein 1 (afap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156228.3

ORF Insert Sequence:

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGATTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAAAAG 
AAGCATGAAC TGAAGATCGT TCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GCGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATCCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATGAAAGG GAAGAAAGGC 
TTGATCACCA ATGGTTTCGG TGCAAAGAAG TTGGACAAGA ATCAACCCAA AAAGGCCAAT 
GGCATTAGCA GCACTCTGCC AGTGAAGCGT AACAACTCCA GTGTAGATCA GTATAAGTAC 
GGGAAAAACA GAGTGGAGGC AGATGCCAAG AAACTCCAAG CCAAAGAGGA GGAGCTGATG 
AGGAAGAAAC AGGAAATCCG AAACCGCCTG ACCCAGCTGA AAAAAGACAG AAAGGACCTC 
CGCACTGCTT TAGAGAACAA CACAGCTAAG CGTTCCCAGG CATCTTTGAC AGAGAGGCTT 
AAGAAAGTGG AGGATGAGTG CAAACTGAAA GAGGAAGAGC GAGTCAGTCT GGAGCTGGAG 
TTGACGGAGG TCAAGGAGAG TCTGAAGAAA GCTCTGAATG GTGGTGTCAC CCTGGGCCTG 
ACCATTGAAC CCAAAACAGG CAGCTCCAGC CCACAGTCTC CAGTATTAAT GAGGCGAACG 
GTGGACAACT CGCCCATTTC CAGCTGTAAC ACCAGTGACA CGGAGACCTG CTCTCTGCCG 
GTCAACAGTG CGTCTCTGCT GCGCCGCCAG ACACAACAGA AAGCCTCACC TGTGCGAGGT 
CACGTCCTCA GAAAAGCTAA GGAATGGGAG ATGAAATCTG GCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_005156228.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156285.1 Danio rerio actin filament associated protein 1 (afap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa17899
Clone ID Related Accession (Same CDS sequence)	XM_005156228.3 , XM_005156228.4
Accession Version	XM_005156228.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	actin filament-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156228.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGATTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAAAAG 
AAGCATGAAC TGAAGATCGT TCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GCGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATCCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATGAAAGG GAAGAAAGGC 
TTGATCACCA ATGGTTTCGG TGCAAAGAAG TTGGACAAGA ATCAACCCAA AAAGGCCAAT 
GGCATTAGCA GCACTCTGCC AGTGAAGCGT AACAACTCCA GTGTAGATCA GTATAAGTAC 
GGGAAAAACA GAGTGGAGGC AGATGCCAAG AAACTCCAAG CCAAAGAGGA GGAGCTGATG 
AGGAAGAAAC AGGAAATCCG AAACCGCCTG ACCCAGCTGA AAAAAGACAG AAAGGACCTC 
CGCACTGCTT TAGAGAACAA CACAGCTAAG CGTTCCCAGG CATCTTTGAC AGAGAGGCTT 
AAGAAAGTGG AGGATGAGTG CAAACTGAAA GAGGAAGAGC GAGTCAGTCT GGAGCTGGAG 
TTGACGGAGG TCAAGGAGAG TCTGAAGAAA GCTCTGAATG GTGGTGTCAC CCTGGGCCTG 
ACCATTGAAC CCAAAACAGG CAGCTCCAGC CCACAGTCTC CAGTATTAAT GAGGCGAACG 
GTGGACAACT CGCCCATTTC CAGCTGTAAC ACCAGTGACA CGGAGACCTG CTCTCTGCCG 
GTCAACAGTG CGTCTCTGCT GCGCCGCCAG ACACAACAGA AAGCCTCACC TGTGCGAGGT 
CACGTCCTCA GAAAAGCTAA GGAATGGGAG ATGAAATCTG GCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156285.1
CDS	386..2593
Translation	MRSDPILFASRAMEELLAELRVFLELLDREYLTAGVREKKQQILNILHRVLATREPSCKTEIHTSLPAPPQMPLPEIPHPWMPPDNGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDQTPQMELLLSGCSITHIPKDGKKKKHELKIVHQGADALVLAVQSKEQAEQWLKVMKEVCSNGNGVVDCDGAGSGSTVHKAELEKKLSCDRPSSDGEPCHENGISDGKDPAKGKKNSKSEQKGTVGRVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCQLKDNQLFLHKDRADLKTHMASLPLRGCEVIPGLDSKHPFAFRLLRNGQEVAVLEASSSESMGRWLGVLLAETGSTTDPAALHYDYIDVETTANVIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGVALHYDDVPCINGTMKGKKGLITNGFGAKKLDKNQPKKANGISSTLPVKRNNSSVDQYKYGKNRVEADAKKLQAKEEELMRKKQEIRNRLTQLKKDRKDLRTALENNTAKRSQASLTERLKKVEDECKLKEEERVSLELELTEVKESLKKALNGGVTLGLTIEPKTGSSSPQSPVLMRRTVDNSPISSCNTSDTETCSLPVNSASLLRRQTQQKASPVRGHVLRKAKEWEMKSGT

Target ORF information:

RefSeq Version	XM_005156228.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin filament associated protein 1 (afap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156228.4

ORF Insert Sequence:

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGATTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAAAAG 
AAGCATGAAC TGAAGATCGT TCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GCGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATCCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATGAAAGG GAAGAAAGGC 
TTGATCACCA ATGGTTTCGG TGCAAAGAAG TTGGACAAGA ATCAACCCAA AAAGGCCAAT 
GGCATTAGCA GCACTCTGCC AGTGAAGCGT AACAACTCCA GTGTAGATCA GTATAAGTAC 
GGGAAAAACA GAGTGGAGGC AGATGCCAAG AAACTCCAAG CCAAAGAGGA GGAGCTGATG 
AGGAAGAAAC AGGAAATCCG AAACCGCCTG ACCCAGCTGA AAAAAGACAG AAAGGACCTC 
CGCACTGCTT TAGAGAACAA CACAGCTAAG CGTTCCCAGG CATCTTTGAC AGAGAGGCTT 
AAGAAAGTGG AGGATGAGTG CAAACTGAAA GAGGAAGAGC GAGTCAGTCT GGAGCTGGAG 
TTGACGGAGG TCAAGGAGAG TCTGAAGAAA GCTCTGAATG GTGGTGTCAC CCTGGGCCTG 
ACCATTGAAC CCAAAACAGG CAGCTCCAGC CCACAGTCTC CAGTATTAAT GAGGCGAACG 
GTGGACAACT CGCCCATTTC CAGCTGTAAC ACCAGTGACA CGGAGACCTG CTCTCTGCCG 
GTCAACAGTG CGTCTCTGCT GCGCCGCCAG ACACAACAGA AAGCCTCACC TGTGCGAGGT 
CACGTCCTCA GAAAAGCTAA GGAATGGGAG ATGAAATCTG GCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_021472533.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328208.1 Danio rerio actin filament associated protein 1 (afap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa145364
Clone ID Related Accession (Same CDS sequence)	XM_021472533.1
Accession Version	XM_021472533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	actin filament-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394840.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGACTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAGAAG 
AAGCATGAAC TGAAGATCGT CCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GTGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATTCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATACCAGA TGCACACTAC 
TCCTCCCCAG CTTCAGGGGG CCGCCGCTGG AAAGACGAAT CTCATTATGA AAATACAGAT 
CTTTATGAAA ACATGCCCTC CCCCAAACTA GCAGCTCGCT ACTCTCCCAA GTCCACCCGC 
ACATCATCTT CCTCGGGGCA TTACAGCACA CCAAACTCCA CCAAATCTCT GTCTTCTTCC 
TCTGCTGCTA ATACTAACAC TAACCAGATG AAAGGGAAGA AAGGCTTGAT CACCAATGGT 
TTCGGTGCAA AGAAGTTGGA CAAGAATCAA CCCAAAAAGG CCAATGGCAT TAGCAGCACT 
CTGCCAGTGA AGCGTAACAA CTCCAGTGTA GATCAGTATA AGTACGGGAA AAACAGAGTG 
GAGGCAGATG CCAAGAAACT CCAAGCCAAA GAGGAGGAGC TGATGAGGAA GAAACAGGAA 
ATCCGAAACC GCCTGACCCA GCTGAAAAAA GACAGAAAGG ACCTCCGCAC TGCTTTAGAG 
AACAACACAG CTAAGCGTTC CCAGGCATCT TTGACAGAGA GGCTTAAGAA AGTGGAGGAT 
GAGTGCAAAC TGAAAGAGGA AGAGCGAGTC AGTCTGGAGC TGGAGTTGAC GGAGGTCAAG 
GAGAGTCTGA AGAAAGCTCT GAATGGTGGT GTCACCCTGG GCCTGACCAT TGAACCCAAA 
ACAGGCAGCT CCAGCCCACA GTCTCCAGTA TTAATGAGGC GAACGGTGGA CAACTCGCCC 
ATTTCCAGCT GTAACACCAG TGACACGGAG ACCTGCTCTC TGCCGGTCAA CAGTGCGTCT 
CTGCTGCGCC GCCAGACACA ACAGAAAGCC TCACCTGTGC GAGGTCACGT CCTCAGAAAA 
GCTAAGGAAT GGGAGATGAA ATCTGGCACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328208.1
CDS	349..2781
Translation	MRSDPILFASRAMEELLAELRVFLELLDREYLTAGVREKKQQILNILHRVLATREPSCKTEIHTSLPAPPQMPLPEIPHPWMPPDNGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDQTPQMELLLSGCSITHIPKDGKKKKHELKIVHQGADALVLAVQSKEQAEQWLKVMKEVCSNGNGVVDCDGAGSGSTVHKAELEKKLSCDRPSSDGEPCHENGISDGKDSAKGKKNSKSEQKGTVGRVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCQLKDNQLFLHKDRADLKTHMASLPLRGCEVIPGLDSKHPFAFRLLRNGQEVAVLEASSSESMGRWLGVLLAETGSTTDPAALHYDYIDVETTANVIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGVALHYDDVPCINGTIPDAHYSSPASGGRRWKDESHYENTDLYENMPSPKLAARYSPKSTRTSSSSGHYSTPNSTKSLSSSSAANTNTNQMKGKKGLITNGFGAKKLDKNQPKKANGISSTLPVKRNNSSVDQYKYGKNRVEADAKKLQAKEEELMRKKQEIRNRLTQLKKDRKDLRTALENNTAKRSQASLTERLKKVEDECKLKEEERVSLELELTEVKESLKKALNGGVTLGLTIEPKTGSSSPQSPVLMRRTVDNSPISSCNTSDTETCSLPVNSASLLRRQTQQKASPVRGHVLRKAKEWEMKSGT

Target ORF information:

RefSeq Version	XM_021472533.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin filament associated protein 1 (afap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472533.1

ORF Insert Sequence:

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGACTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAGAAG 
AAGCATGAAC TGAAGATCGT CCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GTGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATTCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATACCAGA TGCACACTAC 
TCCTCCCCAG CTTCAGGGGG CCGCCGCTGG AAAGACGAAT CTCATTATGA AAATACAGAT 
CTTTATGAAA ACATGCCCTC CCCCAAACTA GCAGCTCGCT ACTCTCCCAA GTCCACCCGC 
ACATCATCTT CCTCGGGGCA TTACAGCACA CCAAACTCCA CCAAATCTCT GTCTTCTTCC 
TCTGCTGCTA ATACTAACAC TAACCAGATG AAAGGGAAGA AAGGCTTGAT CACCAATGGT 
TTCGGTGCAA AGAAGTTGGA CAAGAATCAA CCCAAAAAGG CCAATGGCAT TAGCAGCACT 
CTGCCAGTGA AGCGTAACAA CTCCAGTGTA GATCAGTATA AGTACGGGAA AAACAGAGTG 
GAGGCAGATG CCAAGAAACT CCAAGCCAAA GAGGAGGAGC TGATGAGGAA GAAACAGGAA 
ATCCGAAACC GCCTGACCCA GCTGAAAAAA GACAGAAAGG ACCTCCGCAC TGCTTTAGAG 
AACAACACAG CTAAGCGTTC CCAGGCATCT TTGACAGAGA GGCTTAAGAA AGTGGAGGAT 
GAGTGCAAAC TGAAAGAGGA AGAGCGAGTC AGTCTGGAGC TGGAGTTGAC GGAGGTCAAG 
GAGAGTCTGA AGAAAGCTCT GAATGGTGGT GTCACCCTGG GCCTGACCAT TGAACCCAAA 
ACAGGCAGCT CCAGCCCACA GTCTCCAGTA TTAATGAGGC GAACGGTGGA CAACTCGCCC 
ATTTCCAGCT GTAACACCAG TGACACGGAG ACCTGCTCTC TGCCGGTCAA CAGTGCGTCT 
CTGCTGCGCC GCCAGACACA ACAGAAAGCC TCACCTGTGC GAGGTCACGT CCTCAGAAAA 
GCTAAGGAAT GGGAGATGAA ATCTGGCACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_021472534.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328209.1 Danio rerio actin filament associated protein 1 (afap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa145365
Clone ID Related Accession (Same CDS sequence)	XM_021472534.1
Accession Version	XM_021472534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	actin filament-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394840.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAA GCAACGAAGA AGCGATGGAG GAGTTGTTGG CAGAGTTACG AGTGTTTCTG 
GAGTTGTTGG ACAGAGAGTA TTTGACTGCT GGAGTGAGAG AGAAGAAACA GCAAATATTA 
AACATCCTGC ACAGAGTTCT GGCCACTAGA GAACCCTCAT GTAAGACAGA GATACACACA 
TCTCTCCCGG CCCCTCCCCA GATGCCCCTA CCAGAGATTC CTCACCCCTG GATGCCTCCA 
GACAATGGGC CTCCTCCTTT ACCGAGCTCC TCTCTTCCTG AGGGTTATTA TGAGGAAGCG 
GTTCCACTGG GTCCTGGGAA AGCACCAGAG TACATCACCT CTAATTATGA CTCGGACGCC 
ATGAGCAGTT CGTATGAGTC ATATGATGAG GAAGAGGAGG ATGGGAAGGG TCAGAAGATG 
CGTCACCAGT GGCCATCTGA GGAGGCCTCC ATGGACTTGG TAAAAGATGC ACGTATCTGC 
GCATTCCTGT TGCGAAAGAA ACGCTTTGGT CAGTGGACCA AACTTCTATG TGTCATAAAG 
GACAACAAAC TGTTGTGCTA CAAGTCCTCT AAAGACCAGA CTCCACAGAT GGAGCTCCTT 
CTGTCCGGCT GCAGTATCAC ACACATCCCC AAAGATGGCA AGAAGAAGAA GCATGAACTG 
AAGATCGTCC ATCAGGGAGC TGATGCTCTA GTGCTTGCTG TACAGAGCAA AGAACAGGCC 
GAACAATGGC TAAAGGTGAT GAAAGAAGTC TGCTCGAATG GAAATGGAGT GGTGGATTGC 
GACGGAGCTG GCTCTGGATC CACTGTTCAC AAGGCAGAGC TGGAAAAGAA GCTGTCCTGT 
GATCGGCCCA GTTCAGATGG AGAGCCCTGC CATGAAAACG GCATCTCAGA TGGCAAAGAT 
TCAGCTAAAG GCAAGAAGAA CTCTAAATCA GAGCAGAAAG GCACTGTGGG ACGTGTGACT 
GGAAAGAAAA TCACAAAAAT CATCAGCCTA GGCAAGAAGA AACCATCAAC AGATGAACAG 
ACCTCATCAG CGGAGGAAGA TGTCCCAACA TGTGGCTATC TTAACGTGCT GTCCAACAAC 
CGCTGGAGAG AGCGCTGGTG CCAGCTAAAA GACAACCAGC TGTTTCTGCA CAAAGATCGA 
GCCGATCTGA AGACGCACAT GGCCTCTCTG CCGCTGCGGG GCTGTGAGGT CATTCCAGGC 
CTGGACTCCA AACATCCCTT CGCCTTCCGT CTACTGCGCA ATGGACAGGA GGTGGCTGTT 
CTGGAGGCCT CCTCCTCTGA GAGTATGGGA AGGTGGTTGG GGGTCTTGCT GGCTGAGACG 
GGCTCCACAA CAGATCCCGC TGCACTCCAC TACGATTACA TTGACGTAGA GACCACTGCT 
AACGTCATTC AGCTGGCAAA GCAGTCATTT TGTTTCACCA GTAAGCGTGC AGTTTCTCCT 
AACCCATACC TGGATAATCC GGTCAATGGT TATGCCTGTC CCACTGGGGT CGCTCTGCAT 
TATGACGATG TGCCCTGCAT CAATGGAACG ATACCAGATG CACACTACTC CTCCCCAGCT 
TCAGGGGGCC GCCGCTGGAA AGACGAATCT CATTATGAAA ATACAGATCT TTATGAAAAC 
ATGCCCTCCC CCAAACTAGC AGCTCGCTAC TCTCCCAAGT CCACCCGCAC ATCATCTTCC 
TCGGGGCATT ACAGCACACC AAACTCCACC AAATCTCTGT CTTCTTCCTC TGCTGCTAAT 
ACTAACACTA ACCAGATGAA AGGGAAGAAA GGCTTGATCA CCAATGGTTT CGGTGCAAAG 
AAGTTGGACA AGAATCAACC CAAAAAGGCC AATGGCATTA GCAGCACTCT GCCAGTGAAG 
CGTAACAACT CCAGTGTAGA TCAGTATAAG TACGGGAAAA ACAGAGTGGA GGCAGATGCC 
AAGAAACTCC AAGCCAAAGA GGAGGAGCTG ATGAGGAAGA AACAGGAAAT CCGAAACCGC 
CTGACCCAGC TGAAAAAAGA CAGAAAGGAC CTCCGCACTG CTTTAGAGAA CAACACAGCT 
AAGCGTTCCC AGGCATCTTT GACAGAGAGG CTTAAGAAAG TGGAGGATGA GTGCAAACTG 
AAAGAGGAAG AGCGAGTCAG TCTGGAGCTG GAGTTGACGG AGGTCAAGGA GAGTCTGAAG 
AAAGCTCTGA ATGGTGGTGT CACCCTGGGC CTGACCATTG AACCCAAAAC AGGCAGCTCC 
AGCCCACAGT CTCCAGTATT AATGAGGCGA ACGGTGGACA ACTCGCCCAT TTCCAGCTGT 
AACACCAGTG ACACGGAGAC CTGCTCTCTG CCGGTCAACA GTGCGTCTCT GCTGCGCCGC 
CAGACACAAC AGAAAGCCTC ACCTGTGCGA GGTCACGTCC TCAGAAAAGC TAAGGAATGG 
GAGATGAAAT CTGGCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328209.1
CDS	118..2538
Translation	MTESNEEAMEELLAELRVFLELLDREYLTAGVREKKQQILNILHRVLATREPSCKTEIHTSLPAPPQMPLPEIPHPWMPPDNGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDQTPQMELLLSGCSITHIPKDGKKKKHELKIVHQGADALVLAVQSKEQAEQWLKVMKEVCSNGNGVVDCDGAGSGSTVHKAELEKKLSCDRPSSDGEPCHENGISDGKDSAKGKKNSKSEQKGTVGRVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCQLKDNQLFLHKDRADLKTHMASLPLRGCEVIPGLDSKHPFAFRLLRNGQEVAVLEASSSESMGRWLGVLLAETGSTTDPAALHYDYIDVETTANVIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGVALHYDDVPCINGTIPDAHYSSPASGGRRWKDESHYENTDLYENMPSPKLAARYSPKSTRTSSSSGHYSTPNSTKSLSSSSAANTNTNQMKGKKGLITNGFGAKKLDKNQPKKANGISSTLPVKRNNSSVDQYKYGKNRVEADAKKLQAKEEELMRKKQEIRNRLTQLKKDRKDLRTALENNTAKRSQASLTERLKKVEDECKLKEEERVSLELELTEVKESLKKALNGGVTLGLTIEPKTGSSSPQSPVLMRRTVDNSPISSCNTSDTETCSLPVNSASLLRRQTQQKASPVRGHVLRKAKEWEMKSGT

Target ORF information:

RefSeq Version	XM_021472534.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin filament associated protein 1 (afap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472534.1

ORF Insert Sequence:

ATGACGGAAA GCAACGAAGA AGCGATGGAG GAGTTGTTGG CAGAGTTACG AGTGTTTCTG 
GAGTTGTTGG ACAGAGAGTA TTTGACTGCT GGAGTGAGAG AGAAGAAACA GCAAATATTA 
AACATCCTGC ACAGAGTTCT GGCCACTAGA GAACCCTCAT GTAAGACAGA GATACACACA 
TCTCTCCCGG CCCCTCCCCA GATGCCCCTA CCAGAGATTC CTCACCCCTG GATGCCTCCA 
GACAATGGGC CTCCTCCTTT ACCGAGCTCC TCTCTTCCTG AGGGTTATTA TGAGGAAGCG 
GTTCCACTGG GTCCTGGGAA AGCACCAGAG TACATCACCT CTAATTATGA CTCGGACGCC 
ATGAGCAGTT CGTATGAGTC ATATGATGAG GAAGAGGAGG ATGGGAAGGG TCAGAAGATG 
CGTCACCAGT GGCCATCTGA GGAGGCCTCC ATGGACTTGG TAAAAGATGC ACGTATCTGC 
GCATTCCTGT TGCGAAAGAA ACGCTTTGGT CAGTGGACCA AACTTCTATG TGTCATAAAG 
GACAACAAAC TGTTGTGCTA CAAGTCCTCT AAAGACCAGA CTCCACAGAT GGAGCTCCTT 
CTGTCCGGCT GCAGTATCAC ACACATCCCC AAAGATGGCA AGAAGAAGAA GCATGAACTG 
AAGATCGTCC ATCAGGGAGC TGATGCTCTA GTGCTTGCTG TACAGAGCAA AGAACAGGCC 
GAACAATGGC TAAAGGTGAT GAAAGAAGTC TGCTCGAATG GAAATGGAGT GGTGGATTGC 
GACGGAGCTG GCTCTGGATC CACTGTTCAC AAGGCAGAGC TGGAAAAGAA GCTGTCCTGT 
GATCGGCCCA GTTCAGATGG AGAGCCCTGC CATGAAAACG GCATCTCAGA TGGCAAAGAT 
TCAGCTAAAG GCAAGAAGAA CTCTAAATCA GAGCAGAAAG GCACTGTGGG ACGTGTGACT 
GGAAAGAAAA TCACAAAAAT CATCAGCCTA GGCAAGAAGA AACCATCAAC AGATGAACAG 
ACCTCATCAG CGGAGGAAGA TGTCCCAACA TGTGGCTATC TTAACGTGCT GTCCAACAAC 
CGCTGGAGAG AGCGCTGGTG CCAGCTAAAA GACAACCAGC TGTTTCTGCA CAAAGATCGA 
GCCGATCTGA AGACGCACAT GGCCTCTCTG CCGCTGCGGG GCTGTGAGGT CATTCCAGGC 
CTGGACTCCA AACATCCCTT CGCCTTCCGT CTACTGCGCA ATGGACAGGA GGTGGCTGTT 
CTGGAGGCCT CCTCCTCTGA GAGTATGGGA AGGTGGTTGG GGGTCTTGCT GGCTGAGACG 
GGCTCCACAA CAGATCCCGC TGCACTCCAC TACGATTACA TTGACGTAGA GACCACTGCT 
AACGTCATTC AGCTGGCAAA GCAGTCATTT TGTTTCACCA GTAAGCGTGC AGTTTCTCCT 
AACCCATACC TGGATAATCC GGTCAATGGT TATGCCTGTC CCACTGGGGT CGCTCTGCAT 
TATGACGATG TGCCCTGCAT CAATGGAACG ATACCAGATG CACACTACTC CTCCCCAGCT 
TCAGGGGGCC GCCGCTGGAA AGACGAATCT CATTATGAAA ATACAGATCT TTATGAAAAC 
ATGCCCTCCC CCAAACTAGC AGCTCGCTAC TCTCCCAAGT CCACCCGCAC ATCATCTTCC 
TCGGGGCATT ACAGCACACC AAACTCCACC AAATCTCTGT CTTCTTCCTC TGCTGCTAAT 
ACTAACACTA ACCAGATGAA AGGGAAGAAA GGCTTGATCA CCAATGGTTT CGGTGCAAAG 
AAGTTGGACA AGAATCAACC CAAAAAGGCC AATGGCATTA GCAGCACTCT GCCAGTGAAG 
CGTAACAACT CCAGTGTAGA TCAGTATAAG TACGGGAAAA ACAGAGTGGA GGCAGATGCC 
AAGAAACTCC AAGCCAAAGA GGAGGAGCTG ATGAGGAAGA AACAGGAAAT CCGAAACCGC 
CTGACCCAGC TGAAAAAAGA CAGAAAGGAC CTCCGCACTG CTTTAGAGAA CAACACAGCT 
AAGCGTTCCC AGGCATCTTT GACAGAGAGG CTTAAGAAAG TGGAGGATGA GTGCAAACTG 
AAAGAGGAAG AGCGAGTCAG TCTGGAGCTG GAGTTGACGG AGGTCAAGGA GAGTCTGAAG 
AAAGCTCTGA ATGGTGGTGT CACCCTGGGC CTGACCATTG AACCCAAAAC AGGCAGCTCC 
AGCCCACAGT CTCCAGTATT AATGAGGCGA ACGGTGGACA ACTCGCCCAT TTCCAGCTGT 
AACACCAGTG ACACGGAGAC CTGCTCTCTG CCGGTCAACA GTGCGTCTCT GCTGCGCCGC 
CAGACACAAC AGAAAGCCTC ACCTGTGCGA GGTCACGTCC TCAGAAAAGC TAAGGAATGG 
GAGATGAAAT CTGGCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_021472535.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328210.1 Danio rerio actin filament associated protein 1 (afap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa145366
Clone ID Related Accession (Same CDS sequence)	XM_021472535.1
Accession Version	XM_021472535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	actin filament-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394840.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGACTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAGAAG 
AAGCATGAAC TGAAGATCGT CCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GTGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATTCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATGAAAGG GAAGAAAGGC 
TTGATCACCA ATGGTTTCGG TGCAAAGAAG TTGGACAAGA ATCAACCCAA AAAGGCCAAT 
GGCATTAGCA GCACTCTGCC AGTGAAGCGT AACAACTCCA GTGTAGATCA GTATAAGTAC 
GGGAAAAACA GAGTGGAGGC AGATGCCAAG AAACTCCAAG CCAAAGAGGA GGAGCTGATG 
AGGAAGAAAC AGGAAATCCG AAACCGCCTG ACCCAGCTGA AAAAAGACAG AAAGGACCTC 
CGCACTGCTT TAGAGAACAA CACAGCTAAG CGTTCCCAGG CATCTTTGAC AGAGAGGCTT 
AAGAAAGTGG AGGATGAGTG CAAACTGAAA GAGGAAGAGC GAGTCAGTCT GGAGCTGGAG 
TTGACGGAGG TCAAGGAGAG TCTGAAGAAA GCTCTGAATG GTGGTGTCAC CCTGGGCCTG 
ACCATTGAAC CCAAAACAGG CAGCTCCAGC CCACAGTCTC CAGTATTAAT GAGGCGAACG 
GTGGACAACT CGCCCATTTC CAGCTGTAAC ACCAGTGACA CGGAGACCTG CTCTCTGCCG 
GTCAACAGTG CGTCTCTGCT GCGCCGCCAG ACACAACAGA AAGCCTCACC TGTGCGAGGT 
CACGTCCTCA GAAAAGCTAA GGAATGGGAG ATGAAATCTG GCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328210.1
CDS	347..2554
Translation	MRSDPILFASRAMEELLAELRVFLELLDREYLTAGVREKKQQILNILHRVLATREPSCKTEIHTSLPAPPQMPLPEIPHPWMPPDNGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDQTPQMELLLSGCSITHIPKDGKKKKHELKIVHQGADALVLAVQSKEQAEQWLKVMKEVCSNGNGVVDCDGAGSGSTVHKAELEKKLSCDRPSSDGEPCHENGISDGKDSAKGKKNSKSEQKGTVGRVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCQLKDNQLFLHKDRADLKTHMASLPLRGCEVIPGLDSKHPFAFRLLRNGQEVAVLEASSSESMGRWLGVLLAETGSTTDPAALHYDYIDVETTANVIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGVALHYDDVPCINGTMKGKKGLITNGFGAKKLDKNQPKKANGISSTLPVKRNNSSVDQYKYGKNRVEADAKKLQAKEEELMRKKQEIRNRLTQLKKDRKDLRTALENNTAKRSQASLTERLKKVEDECKLKEEERVSLELELTEVKESLKKALNGGVTLGLTIEPKTGSSSPQSPVLMRRTVDNSPISSCNTSDTETCSLPVNSASLLRRQTQQKASPVRGHVLRKAKEWEMKSGT

Target ORF information:

RefSeq Version	XM_021472535.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin filament associated protein 1 (afap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472535.1

ORF Insert Sequence:

ATGCGGAGCG ACCCCATACT GTTCGCCTCG AGGGCGATGG AGGAGTTGTT GGCAGAGTTA 
CGAGTGTTTC TGGAGTTGTT GGACAGAGAG TATTTGACTG CTGGAGTGAG AGAGAAGAAA 
CAGCAAATAT TAAACATCCT GCACAGAGTT CTGGCCACTA GAGAACCCTC ATGTAAGACA 
GAGATACACA CATCTCTCCC GGCCCCTCCC CAGATGCCCC TACCAGAGAT TCCTCACCCC 
TGGATGCCTC CAGACAATGG GCCTCCTCCT TTACCGAGCT CCTCTCTTCC TGAGGGTTAT 
TATGAGGAAG CGGTTCCACT GGGTCCTGGG AAAGCACCAG AGTACATCAC CTCTAATTAT 
GACTCGGACG CCATGAGCAG TTCGTATGAG TCATATGATG AGGAAGAGGA GGATGGGAAG 
GGTCAGAAGA TGCGTCACCA GTGGCCATCT GAGGAGGCCT CCATGGACTT GGTAAAAGAT 
GCACGTATCT GCGCATTCCT GTTGCGAAAG AAACGCTTTG GTCAGTGGAC CAAACTTCTA 
TGTGTCATAA AGGACAACAA ACTGTTGTGC TACAAGTCCT CTAAAGACCA GACTCCACAG 
ATGGAGCTCC TTCTGTCCGG CTGCAGTATC ACACACATCC CCAAAGATGG CAAGAAGAAG 
AAGCATGAAC TGAAGATCGT CCATCAGGGA GCTGATGCTC TAGTGCTTGC TGTACAGAGC 
AAAGAACAGG CCGAACAATG GCTAAAGGTG ATGAAAGAAG TCTGCTCGAA TGGAAATGGA 
GTGGTGGATT GCGACGGAGC TGGCTCTGGA TCCACTGTTC ACAAGGCAGA GCTGGAAAAG 
AAGCTGTCCT GTGATCGGCC CAGTTCAGAT GGAGAGCCCT GCCATGAAAA CGGCATCTCA 
GATGGCAAAG ATTCAGCTAA AGGCAAGAAG AACTCTAAAT CAGAGCAGAA AGGCACTGTG 
GGACGTGTGA CTGGAAAGAA AATCACAAAA ATCATCAGCC TAGGCAAGAA GAAACCATCA 
ACAGATGAAC AGACCTCATC AGCGGAGGAA GATGTCCCAA CATGTGGCTA TCTTAACGTG 
CTGTCCAACA ACCGCTGGAG AGAGCGCTGG TGCCAGCTAA AAGACAACCA GCTGTTTCTG 
CACAAAGATC GAGCCGATCT GAAGACGCAC ATGGCCTCTC TGCCGCTGCG GGGCTGTGAG 
GTCATTCCAG GCCTGGACTC CAAACATCCC TTCGCCTTCC GTCTACTGCG CAATGGACAG 
GAGGTGGCTG TTCTGGAGGC CTCCTCCTCT GAGAGTATGG GAAGGTGGTT GGGGGTCTTG 
CTGGCTGAGA CGGGCTCCAC AACAGATCCC GCTGCACTCC ACTACGATTA CATTGACGTA 
GAGACCACTG CTAACGTCAT TCAGCTGGCA AAGCAGTCAT TTTGTTTCAC CAGTAAGCGT 
GCAGTTTCTC CTAACCCATA CCTGGATAAT CCGGTCAATG GTTATGCCTG TCCCACTGGG 
GTCGCTCTGC ATTATGACGA TGTGCCCTGC ATCAATGGAA CGATGAAAGG GAAGAAAGGC 
TTGATCACCA ATGGTTTCGG TGCAAAGAAG TTGGACAAGA ATCAACCCAA AAAGGCCAAT 
GGCATTAGCA GCACTCTGCC AGTGAAGCGT AACAACTCCA GTGTAGATCA GTATAAGTAC 
GGGAAAAACA GAGTGGAGGC AGATGCCAAG AAACTCCAAG CCAAAGAGGA GGAGCTGATG 
AGGAAGAAAC AGGAAATCCG AAACCGCCTG ACCCAGCTGA AAAAAGACAG AAAGGACCTC 
CGCACTGCTT TAGAGAACAA CACAGCTAAG CGTTCCCAGG CATCTTTGAC AGAGAGGCTT 
AAGAAAGTGG AGGATGAGTG CAAACTGAAA GAGGAAGAGC GAGTCAGTCT GGAGCTGGAG 
TTGACGGAGG TCAAGGAGAG TCTGAAGAAA GCTCTGAATG GTGGTGTCAC CCTGGGCCTG 
ACCATTGAAC CCAAAACAGG CAGCTCCAGC CCACAGTCTC CAGTATTAAT GAGGCGAACG 
GTGGACAACT CGCCCATTTC CAGCTGTAAC ACCAGTGACA CGGAGACCTG CTCTCTGCCG 
GTCAACAGTG CGTCTCTGCT GCGCCGCCAG ACACAACAGA AAGCCTCACC TGTGCGAGGT 
CACGTCCTCA GAAAAGCTAA GGAATGGGAG ATGAAATCTG GCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	afap1
Entrez Gene ID	568356
Full Name	actin filament associated protein 1
Synonyms	im:7148276
General protein information	Preferred Names actin filament associated protein 1 Names actin filament-associated protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

	LOC709419	XP_002804046.1
Xenopus tropicalis (tropical clawed frog)	afap1	XP_004911301.1
Mus musculus (house mouse)	Afap1	NP_081649.1
Gallus gallus (chicken)	AFAP1	NP_989536.1
Bos taurus (cattle)	AFAP1	NP_001192973.1
Rattus norvegicus (Norway rat)	Afap1	NP_543176.1
Danio rerio (zebrafish)	afap1	XP_005156284.1
Homo sapiens (human)	AFAP1	NP_001128119.1
Pan troglodytes (chimpanzee)	AFAP1	XP_003310294.1
Canis lupus familiaris (dog)	AFAP1	XP_005618516.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

afap1 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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