plekha5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	plekha5
Entrez Gene ID	567300
Full Name	pleckstrin homology domain containing, family A member 5
Synonyms	si:ch211-239h19.1,wu:fb16e08
General protein information	Preferred Names pleckstrin homology domain containing, family A member 5 Names pleckstrin homology domain-containing family A member 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021475078.1	XP_021330753.1	pleckstrin homology domain-containing family A member 5 isoform X2
XM_021475084.1	XP_021330759.1	pleckstrin homology domain-containing family A member 5 isoform X9
XM_021475086.1	XP_021330761.1	pleckstrin homology domain-containing family A member 5 isoform X11
XM_021475087.1	XP_021330762.1	pleckstrin homology domain-containing family A member 5 isoform X12
XM_009300003.2	XP_009298278.1	pleckstrin homology domain-containing family A member 5 isoform X13
XM_021475091.1	XP_021330766.1	pleckstrin homology domain-containing family A member 5 isoform X18
XM_009300000.2	XP_009298275.1	pleckstrin homology domain-containing family A member 5 isoform X4
XM_009300011.2	XP_009298286.1	pleckstrin homology domain-containing family A member 5 isoform X15
XM_009299999.2	XP_009298274.1	pleckstrin homology domain-containing family A member 5 isoform X3
XM_009300009.2	XP_009298284.1	pleckstrin homology domain-containing family A member 5 isoform X13
XM_009300010.2	XP_009298285.1	pleckstrin homology domain-containing family A member 5 isoform X14
XM_021475082.1	XP_021330757.1	pleckstrin homology domain-containing family A member 5 isoform X7
XM_021475089.1	XP_021330764.1	pleckstrin homology domain-containing family A member 5 isoform X15
XM_005164479.4	XP_005164536.1	pleckstrin homology domain-containing family A member 5 isoform X17
XM_009300008.2	XP_009298283.1	pleckstrin homology domain-containing family A member 5 isoform X11
XM_005164474.3	XP_005164531.1	pleckstrin homology domain-containing family A member 5 isoform X1
XM_009299998.2	XP_009298273.1	pleckstrin homology domain-containing family A member 5 isoform X2
XM_021475077.1	XP_021330752.1	pleckstrin homology domain-containing family A member 5 isoform X1
XM_021475079.1	XP_021330754.1	pleckstrin homology domain-containing family A member 5 isoform X3
XM_021475088.1	XP_021330763.1	pleckstrin homology domain-containing family A member 5 isoform X14
XM_009300010.3	XP_009298285.1	pleckstrin homology domain-containing family A member 5 isoform X19
XM_009300002.2	XP_009298277.1	pleckstrin homology domain-containing family A member 5 isoform X6
XM_009300005.2	XP_009298280.1	pleckstrin homology domain-containing family A member 5 isoform X9
XM_009300006.2	XP_009298281.1	pleckstrin homology domain-containing family A member 5 isoform X10
XM_005164479.3	XP_005164536.1	pleckstrin homology domain-containing family A member 5 isoform X12
XM_005164474.4	XP_005164531.1	pleckstrin homology domain-containing family A member 5 isoform X4
XM_021475081.1	XP_021330756.1	pleckstrin homology domain-containing family A member 5 isoform X6
XM_021475085.1	XP_021330760.1	pleckstrin homology domain-containing family A member 5 isoform X10
XM_021475090.1	XP_021330765.1	pleckstrin homology domain-containing family A member 5 isoform X16
XM_009300011.3	XP_009298286.1	pleckstrin homology domain-containing family A member 5 isoform X21
XM_021475093.1	XP_021330768.1	pleckstrin homology domain-containing family A member 5 isoform X22
XM_009300001.2	XP_009298276.1	pleckstrin homology domain-containing family A member 5 isoform X5
XM_009300003.1	XP_009298278.1	pleckstrin homology domain-containing family A member 5 isoform X7
XM_009300004.2	XP_009298279.1	pleckstrin homology domain-containing family A member 5 isoform X8
XM_009300012.2	XP_009298287.1	pleckstrin homology domain-containing family A member 5 isoform X16
NM_001327786.1	NP_001314715.1	pleckstrin homology domain-containing family A member 5
XM_021475080.1	XP_021330755.1	pleckstrin homology domain-containing family A member 5 isoform X5
XM_021475083.1	XP_021330758.1	pleckstrin homology domain-containing family A member 5 isoform X8
XM_009299999.3	XP_009298274.1	pleckstrin homology domain-containing family A member 5 isoform X20

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Plekha5	NP_659169.3
Canis lupus familiaris (dog)	PLEKHA5	XP_005637143.1
Pan troglodytes (chimpanzee)	PLEKHA5	XP_520779.3
Bos taurus (cattle)	PLEKHA5	XP_002687800.3
Rattus norvegicus (Norway rat)	Plekha5	NP_647556.1
Gallus gallus (chicken)	PLEKHA5	XP_004938029.1
Danio rerio (zebrafish)	plekha5	XP_005164532.1
Homo sapiens (human)	PLEKHA5	NP_001243399.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.
Ma Z, Zhu P, Pang M, Guo L, Chang N, Zheng J, Zhu X, Gao C, Huang H, Cui Z, Xiong JW, Peng J, Chen J
Scientific reports7(1)10381(2017 Sep)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A novel neuropeptide in suppressing luteinizing hormone release in goldfish, Carassius auratus.
Liu Y, Li S, Qi X, Zhou W, Liu X, Lin H, Zhang Y, Cheng CH
Molecular and cellular endocrinology374(1-2)65-72(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following plekha5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plekha5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa09067	NM_001327786.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa09061	XM_005164474.4 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa09066	XM_005164479.4 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142020	XM_021475077.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa142021	XM_021475078.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa142022	XM_021475079.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa142023	XM_021475080.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa142024	XM_021475081.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa142025	XM_021475082.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa142026	XM_021475083.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa142027	XM_021475084.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa142028	XM_021475085.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142029	XM_021475086.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142030	XM_021475087.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142031	XM_021475088.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142032	XM_021475089.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142033	XM_021475090.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142034	XM_021475091.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa142035	XM_021475093.1 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa45107	XM_009299998.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa45108	XM_009299999.3 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa45109	XM_009300000.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa45110	XM_009300001.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa45111	XM_009300002.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45112	XM_009300003.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45113	XM_009300004.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45114	XM_009300005.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45115	XM_009300006.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45116	XM_009300008.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45117	XM_009300009.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45118	XM_009300010.3 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45119	XM_009300011.3 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45120	XM_009300012.2 Latest version!	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
ODa45118	XM_009300010.2	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa09066	XM_005164479.3	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa09061	XM_005164474.3	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa45119	XM_009300011.2	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45112	XM_009300003.1	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45108	XM_009299999.2	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plekha5 ( NM_001327786.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001314715.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), mRNA.

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CloneID	ODa09067
Clone ID Related Accession (Same CDS sequence)	NM_001327786.1
Accession Version	NM_001327786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from LO018342.1, BX088564.10 and BX914207.15. On May 25, 2016 this sequence version replaced XM_690591.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC155854.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG 
TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA 
GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG 
ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC 
CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG 
CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC 
GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG 
ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT 
GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT 
GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGGCCCGG ATTACAGGCT GTATAAGAGT 
GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG 
CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG 
GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC 
TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC 
GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG 
CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT 
GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA 
CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT 
GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT 
AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC 
TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG 
GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG 
ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG 
AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA 
CTGATCTCAG TGAACAGTGT GTCGACTCCT CCTCAATCTC CAAGCTCCTC CTCCTCTCCT 
CCGCCTCCAC CCTCCCCTCC ACAGCTGACG GACGGGTCAC ACTTCATGTG TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314715.1
CDS	21..3737
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISVNSVSTPPQSPSSSSSPPPPPSPPQLTDGSHFMCV

Target ORF information:

RefSeq Version	NM_001327786.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001327786.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG 
TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA 
GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG 
ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC 
CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG 
CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC 
GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG 
ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT 
GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT 
GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGGCCCGG ATTACAGGCT GTATAAGAGT 
GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG 
CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG 
GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC 
TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC 
GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG 
CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT 
GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA 
CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT 
GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT 
AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC 
TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG 
GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG 
ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG 
AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA 
CTGATCTCAG TGAACAGTGT GTCGACTCCT CCTCAATCTC CAAGCTCCTC CTCCTCTCCT 
CCGCCTCCAC CCTCCCCTCC ACAGCTGACG GACGGGTCAC ACTTCATGTG TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_005164474.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164531.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X1, mRNA.

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CloneID	ODa09061
Clone ID Related Accession (Same CDS sequence)	XM_005164474.3 , XM_005164474.4
Accession Version	XM_005164474.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164474.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA 
GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC 
TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA 
AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA 
AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG 
AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT 
GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC 
CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG 
TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA 
CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC 
TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA 
CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG 
GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG 
AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA 
GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA 
GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC 
ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC 
AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG 
GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA 
GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG 
GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG 
CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC 
ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT 
CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG 
CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG 
CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG 
CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC 
ACACTGATCT CAGCATCATG TGACAGGAAG AACACATCAT CACCATCGTC ATCATCAGAC 
TGGAAGTCTC GCCCCCTCCT TCATCAGCTG CCCAACACAA ACACAACAGC GTCCAGGCCT 
CAGTTCAACA GCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164531.1
CDS	44..4201
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_005164474.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164474.3

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA 
GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC 
TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA 
AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA 
AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG 
AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT 
GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC 
CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG 
TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA 
CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC 
TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA 
CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG 
GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG 
AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA 
GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA 
GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC 
ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC 
AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG 
GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA 
GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG 
GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG 
CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC 
ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT 
CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG 
CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG 
CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG 
CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC 
ACACTGATCT CAGCATCATG TGACAGGAAG AACACATCAT CACCATCGTC ATCATCAGAC 
TGGAAGTCTC GCCCCCTCCT TCATCAGCTG CCCAACACAA ACACAACAGC GTCCAGGCCT 
CAGTTCAACA GCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_005164474.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164531.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa09061
Clone ID Related Accession (Same CDS sequence)	XM_005164474.3 , XM_005164474.4
Accession Version	XM_005164474.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164474.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA 
GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC 
TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA 
AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA 
AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG 
AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT 
GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC 
CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG 
TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA 
CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC 
TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA 
CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG 
GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG 
AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA 
GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA 
GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC 
ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC 
AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG 
GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA 
GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG 
GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG 
CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC 
ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT 
CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG 
CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG 
CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG 
CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC 
ACACTGATCT CAGCATCATG TGACAGGAAG AACACATCAT CACCATCGTC ATCATCAGAC 
TGGAAGTCTC GCCCCCTCCT TCATCAGCTG CCCAACACAA ACACAACAGC GTCCAGGCCT 
CAGTTCAACA GCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164531.1
CDS	42..4199
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_005164474.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164474.4

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA 
GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC 
TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA 
AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA 
AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG 
AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT 
GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC 
CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG 
TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA 
CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC 
TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA 
CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG 
GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG 
AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA 
GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA 
GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC 
ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC 
AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG 
GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA 
GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG 
GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG 
CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC 
ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT 
CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG 
CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG 
CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG 
CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC 
ACACTGATCT CAGCATCATG TGACAGGAAG AACACATCAT CACCATCGTC ATCATCAGAC 
TGGAAGTCTC GCCCCCTCCT TCATCAGCTG CCCAACACAA ACACAACAGC GTCCAGGCCT 
CAGTTCAACA GCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_005164479.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164536.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X19, mRNA.

Price & Availability↑

CloneID	ODa09066
Clone ID Related Accession (Same CDS sequence)	XM_005164479.4 , XM_005164479.3
Accession Version	XM_005164479.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164479.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAAG CCTCAAACTA CACCGGCGGC TCCGACTACA CCACACATCC CAGCAGCCCC 
ACAACCAGAC CATCCCGGTC CTCAAAGAAA GTGCACAACT TTGGGAAAAG GTCCAACTCC 
ATCAAGAGGA ACCCCAATGC ACCTGTGATC AAAAACGGCT GGCTTCACAA ACAGGACAGC 
ACTGGGATGA AGATGTGGAA GAAGAGATGG TTCGTTCTGT CTGATATGTG CCTCTTCTAC 
TACAGAGATG AGAAGGAAGA GGGGATTCTG GGAAGCATCC TCCTGCCTAG CTTTCACATC 
TCCATGCTGT CCGTGGACGA CCATATTACC CGGAAATACG CCTTCAAGGC GACGCATCCA 
AACATGCGGA CGTATTATTT CAGCACAGAC ACAGCCAAAG ATATGGAGTC CTGGATGAAA 
GTGATGTCGG ATGCGGCGAT GGCTCACAGC GAGCCAATCA GAAGGCTTGA GAAGGTAAAG 
CTGGACTCTC GCGTTCCTCA GGAGATGAAC AACATGCTGA ACCACCGCGT CTTGACACGA 
CCCGAGATCC AAAACAACGA GAGGAACCGC GAGACGCTCC GCCAAGAGGA CAAGAAGAGC 
AAACCATCAG AGAAACCTCA GAAAGACAAA GACAGAGTCA GCATGCAGAA AGACGGAGAG 
CGCTTCAGTG AAAACTACAC TCACCGGCAG AAAGACGGCG AGCGCTACAT GCTGCAGAAA 
GACCTGGAGA AATTTGTCCT GCAGAAAGAC GGAGAGAAGT ACGCCGTGCA GAAAGAAGGC 
GACAAATATC TCCTGCAGAA AGACGGACAC AAATACACAG TGCACAAAGA CGGAGACAAA 
TACACGCTTC AGAGAGAACT CGAGATGTAC AATCCACCAA AAGACGCGGA GAAGATCCCG 
CAGAAAGATG CAAGGTTTAG TCTGGTGCAG AAGGAGCTGG AGAAACCCGT CCCGCCACCG 
CGATCTGAGG AAAAATACGG CTTTCAGAAA GAGGGCACCA TTGAGCGTCC GCTAACTAAA 
ATAAATAGCA TTAAACTGCA GCCGGCTCAA GCAGCCGCCA TCGCTGCAGC CGTTTCATCC 
TCTAGGATGA TGCAGAAACA GGTTAATGGC TCCGGGGAAC GAGCGGGAGA CAGAAGTCCT 
GGAGATTTGA GTACTGTGAG GACGAGCGTC CCGCCACCAA CGCAGCCGGC TCCAGAACCA 
GAGCGAAGCC TCAGCAGAAC CAGCTCAATG CAGCAGCTGG AGCAGTGGGT TCGCACACAC 
CGCACACGAG CTCCAGACGA CGACACCAGG AGCATTACGT CCTACCAGAC CCTGCCAAGG 
AACATGCCGA GCCACCGGGC GCAGATTGTC CCTCGCTACC CTGAGGGCTA CCGCACTCTG 
CCCCGAAACA TGCTGCGTCC GGACAGCATC TGCAGTGTAG CAGGATCAGT GTACGACCGA 
GCGCTTCAGC CCAGCACGCA GGAAAAGCGG CGCTCCATCC GAGATGACAC CATGTGGCAG 
CTGTACGAGT GGCAGCAGCG GCAGGCGCAC AGTCGGCTTG GGTACGGGAC GCTGCCCAGT 
CCCAAAACTA TGGGGCAGAT CGCGGAGTCC ATCCCCACGT CCCCATCCCA TGGCTCTCTG 
GCCGGATATC ACACCTTCTC CCCGAACAGA CCGCTCAACC CTGACTCGCG CTCGGAGCTG 
TCTTCGCCTG TGTTTCGTGG AGACGTGACC ATCGAGCGAC GCCACCGACC ACACCTGTCC 
AAGTACTCCT ATCCTGCGGA ACGCAGAGCT GCTCCTCCCG CACAGAACAT CACTGCACAG 
TCTCTTCAAG GCAAAACGCC AGAGGAACTG ACCCTGCTGC TCATCAAACT GCGGCGGCAG 
CAGGCCGAGC TCAACAGCCT CCGTGAGCAC ACACTCGCGC AGCTCATGCA GCTGAACCTC 
GACGCCACCA ACCCAAAGAG TGAAATCCTC TCTCATCACC TGCAGAGGAA TCTCATGTAC 
TTGGACAGTC AGATGAAGGA GAACGAACCC ATAATCTTCA TGATTCACAC TATGATTGAG 
AACTCGGCGC CCCGGCCGCA GCTCTACCAG CAACAGATGA GTCCTGAAGA GTACAGAGAA 
AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT 
GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG 
CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC 
CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC 
ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT 
CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC 
GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG 
AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC 
AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC 
TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG 
CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC 
CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT 
GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG 
CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG 
GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC 
TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC 
GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG 
CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT 
GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA 
CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT 
GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT 
AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC 
TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG 
GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG 
ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG 
AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA 
CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG 
AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG 
TTCAACAGCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164536.1
CDS	329..4123
Translation	MSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_005164479.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164479.4

ORF Insert Sequence:

ATGAGCGAAG CCTCAAACTA CACCGGCGGC TCCGACTACA CCACACATCC CAGCAGCCCC 
ACAACCAGAC CATCCCGGTC CTCAAAGAAA GTGCACAACT TTGGGAAAAG GTCCAACTCC 
ATCAAGAGGA ACCCCAATGC ACCTGTGATC AAAAACGGCT GGCTTCACAA ACAGGACAGC 
ACTGGGATGA AGATGTGGAA GAAGAGATGG TTCGTTCTGT CTGATATGTG CCTCTTCTAC 
TACAGAGATG AGAAGGAAGA GGGGATTCTG GGAAGCATCC TCCTGCCTAG CTTTCACATC 
TCCATGCTGT CCGTGGACGA CCATATTACC CGGAAATACG CCTTCAAGGC GACGCATCCA 
AACATGCGGA CGTATTATTT CAGCACAGAC ACAGCCAAAG ATATGGAGTC CTGGATGAAA 
GTGATGTCGG ATGCGGCGAT GGCTCACAGC GAGCCAATCA GAAGGCTTGA GAAGGTAAAG 
CTGGACTCTC GCGTTCCTCA GGAGATGAAC AACATGCTGA ACCACCGCGT CTTGACACGA 
CCCGAGATCC AAAACAACGA GAGGAACCGC GAGACGCTCC GCCAAGAGGA CAAGAAGAGC 
AAACCATCAG AGAAACCTCA GAAAGACAAA GACAGAGTCA GCATGCAGAA AGACGGAGAG 
CGCTTCAGTG AAAACTACAC TCACCGGCAG AAAGACGGCG AGCGCTACAT GCTGCAGAAA 
GACCTGGAGA AATTTGTCCT GCAGAAAGAC GGAGAGAAGT ACGCCGTGCA GAAAGAAGGC 
GACAAATATC TCCTGCAGAA AGACGGACAC AAATACACAG TGCACAAAGA CGGAGACAAA 
TACACGCTTC AGAGAGAACT CGAGATGTAC AATCCACCAA AAGACGCGGA GAAGATCCCG 
CAGAAAGATG CAAGGTTTAG TCTGGTGCAG AAGGAGCTGG AGAAACCCGT CCCGCCACCG 
CGATCTGAGG AAAAATACGG CTTTCAGAAA GAGGGCACCA TTGAGCGTCC GCTAACTAAA 
ATAAATAGCA TTAAACTGCA GCCGGCTCAA GCAGCCGCCA TCGCTGCAGC CGTTTCATCC 
TCTAGGATGA TGCAGAAACA GGTTAATGGC TCCGGGGAAC GAGCGGGAGA CAGAAGTCCT 
GGAGATTTGA GTACTGTGAG GACGAGCGTC CCGCCACCAA CGCAGCCGGC TCCAGAACCA 
GAGCGAAGCC TCAGCAGAAC CAGCTCAATG CAGCAGCTGG AGCAGTGGGT TCGCACACAC 
CGCACACGAG CTCCAGACGA CGACACCAGG AGCATTACGT CCTACCAGAC CCTGCCAAGG 
AACATGCCGA GCCACCGGGC GCAGATTGTC CCTCGCTACC CTGAGGGCTA CCGCACTCTG 
CCCCGAAACA TGCTGCGTCC GGACAGCATC TGCAGTGTAG CAGGATCAGT GTACGACCGA 
GCGCTTCAGC CCAGCACGCA GGAAAAGCGG CGCTCCATCC GAGATGACAC CATGTGGCAG 
CTGTACGAGT GGCAGCAGCG GCAGGCGCAC AGTCGGCTTG GGTACGGGAC GCTGCCCAGT 
CCCAAAACTA TGGGGCAGAT CGCGGAGTCC ATCCCCACGT CCCCATCCCA TGGCTCTCTG 
GCCGGATATC ACACCTTCTC CCCGAACAGA CCGCTCAACC CTGACTCGCG CTCGGAGCTG 
TCTTCGCCTG TGTTTCGTGG AGACGTGACC ATCGAGCGAC GCCACCGACC ACACCTGTCC 
AAGTACTCCT ATCCTGCGGA ACGCAGAGCT GCTCCTCCCG CACAGAACAT CACTGCACAG 
TCTCTTCAAG GCAAAACGCC AGAGGAACTG ACCCTGCTGC TCATCAAACT GCGGCGGCAG 
CAGGCCGAGC TCAACAGCCT CCGTGAGCAC ACACTCGCGC AGCTCATGCA GCTGAACCTC 
GACGCCACCA ACCCAAAGAG TGAAATCCTC TCTCATCACC TGCAGAGGAA TCTCATGTAC 
TTGGACAGTC AGATGAAGGA GAACGAACCC ATAATCTTCA TGATTCACAC TATGATTGAG 
AACTCGGCGC CCCGGCCGCA GCTCTACCAG CAACAGATGA GTCCTGAAGA GTACAGAGAA 
AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT 
GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG 
CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC 
CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC 
ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT 
CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC 
GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG 
AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC 
AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC 
TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG 
CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC 
CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT 
GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG 
CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG 
GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC 
TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC 
GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG 
CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT 
GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA 
CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT 
GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT 
AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC 
TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG 
GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG 
ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG 
AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA 
CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG 
AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG 
TTCAACAGCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_005164479.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164536.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X12, mRNA.

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CloneID	ODa09066
Clone ID Related Accession (Same CDS sequence)	XM_005164479.4 , XM_005164479.3
Accession Version	XM_005164479.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164479.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAAG CCTCAAACTA CACCGGCGGC TCCGACTACA CCACACATCC CAGCAGCCCC 
ACAACCAGAC CATCCCGGTC CTCAAAGAAA GTGCACAACT TTGGGAAAAG GTCCAACTCC 
ATCAAGAGGA ACCCCAATGC ACCTGTGATC AAAAACGGCT GGCTTCACAA ACAGGACAGC 
ACTGGGATGA AGATGTGGAA GAAGAGATGG TTCGTTCTGT CTGATATGTG CCTCTTCTAC 
TACAGAGATG AGAAGGAAGA GGGGATTCTG GGAAGCATCC TCCTGCCTAG CTTTCACATC 
TCCATGCTGT CCGTGGACGA CCATATTACC CGGAAATACG CCTTCAAGGC GACGCATCCA 
AACATGCGGA CGTATTATTT CAGCACAGAC ACAGCCAAAG ATATGGAGTC CTGGATGAAA 
GTGATGTCGG ATGCGGCGAT GGCTCACAGC GAGCCAATCA GAAGGCTTGA GAAGGTAAAG 
CTGGACTCTC GCGTTCCTCA GGAGATGAAC AACATGCTGA ACCACCGCGT CTTGACACGA 
CCCGAGATCC AAAACAACGA GAGGAACCGC GAGACGCTCC GCCAAGAGGA CAAGAAGAGC 
AAACCATCAG AGAAACCTCA GAAAGACAAA GACAGAGTCA GCATGCAGAA AGACGGAGAG 
CGCTTCAGTG AAAACTACAC TCACCGGCAG AAAGACGGCG AGCGCTACAT GCTGCAGAAA 
GACCTGGAGA AATTTGTCCT GCAGAAAGAC GGAGAGAAGT ACGCCGTGCA GAAAGAAGGC 
GACAAATATC TCCTGCAGAA AGACGGACAC AAATACACAG TGCACAAAGA CGGAGACAAA 
TACACGCTTC AGAGAGAACT CGAGATGTAC AATCCACCAA AAGACGCGGA GAAGATCCCG 
CAGAAAGATG CAAGGTTTAG TCTGGTGCAG AAGGAGCTGG AGAAACCCGT CCCGCCACCG 
CGATCTGAGG AAAAATACGG CTTTCAGAAA GAGGGCACCA TTGAGCGTCC GCTAACTAAA 
ATAAATAGCA TTAAACTGCA GCCGGCTCAA GCAGCCGCCA TCGCTGCAGC CGTTTCATCC 
TCTAGGATGA TGCAGAAACA GGTTAATGGC TCCGGGGAAC GAGCGGGAGA CAGAAGTCCT 
GGAGATTTGA GTACTGTGAG GACGAGCGTC CCGCCACCAA CGCAGCCGGC TCCAGAACCA 
GAGCGAAGCC TCAGCAGAAC CAGCTCAATG CAGCAGCTGG AGCAGTGGGT TCGCACACAC 
CGCACACGAG CTCCAGACGA CGACACCAGG AGCATTACGT CCTACCAGAC CCTGCCAAGG 
AACATGCCGA GCCACCGGGC GCAGATTGTC CCTCGCTACC CTGAGGGCTA CCGCACTCTG 
CCCCGAAACA TGCTGCGTCC GGACAGCATC TGCAGTGTAG CAGGATCAGT GTACGACCGA 
GCGCTTCAGC CCAGCACGCA GGAAAAGCGG CGCTCCATCC GAGATGACAC CATGTGGCAG 
CTGTACGAGT GGCAGCAGCG GCAGGCGCAC AGTCGGCTTG GGTACGGGAC GCTGCCCAGT 
CCCAAAACTA TGGGGCAGAT CGCGGAGTCC ATCCCCACGT CCCCATCCCA TGGCTCTCTG 
GCCGGATATC ACACCTTCTC CCCGAACAGA CCGCTCAACC CTGACTCGCG CTCGGAGCTG 
TCTTCGCCTG TGTTTCGTGG AGACGTGACC ATCGAGCGAC GCCACCGACC ACACCTGTCC 
AAGTACTCCT ATCCTGCGGA ACGCAGAGCT GCTCCTCCCG CACAGAACAT CACTGCACAG 
TCTCTTCAAG GCAAAACGCC AGAGGAACTG ACCCTGCTGC TCATCAAACT GCGGCGGCAG 
CAGGCCGAGC TCAACAGCCT CCGTGAGCAC ACACTCGCGC AGCTCATGCA GCTGAACCTC 
GACGCCACCA ACCCAAAGAG TGAAATCCTC TCTCATCACC TGCAGAGGAA TCTCATGTAC 
TTGGACAGTC AGATGAAGGA GAACGAACCC ATAATCTTCA TGATTCACAC TATGATTGAG 
AACTCGGCGC CCCGGCCGCA GCTCTACCAG CAACAGATGA GTCCTGAAGA GTACAGAGAA 
AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT 
GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG 
CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC 
CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC 
ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT 
CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC 
GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG 
AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC 
AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC 
TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG 
CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC 
CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT 
GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG 
CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG 
GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC 
TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC 
GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG 
CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT 
GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA 
CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT 
GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT 
AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC 
TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG 
GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG 
ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG 
AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA 
CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG 
AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG 
TTCAACAGCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164536.1
CDS	332..4126
Translation	MSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_005164479.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164479.3

ORF Insert Sequence:

ATGAGCGAAG CCTCAAACTA CACCGGCGGC TCCGACTACA CCACACATCC CAGCAGCCCC 
ACAACCAGAC CATCCCGGTC CTCAAAGAAA GTGCACAACT TTGGGAAAAG GTCCAACTCC 
ATCAAGAGGA ACCCCAATGC ACCTGTGATC AAAAACGGCT GGCTTCACAA ACAGGACAGC 
ACTGGGATGA AGATGTGGAA GAAGAGATGG TTCGTTCTGT CTGATATGTG CCTCTTCTAC 
TACAGAGATG AGAAGGAAGA GGGGATTCTG GGAAGCATCC TCCTGCCTAG CTTTCACATC 
TCCATGCTGT CCGTGGACGA CCATATTACC CGGAAATACG CCTTCAAGGC GACGCATCCA 
AACATGCGGA CGTATTATTT CAGCACAGAC ACAGCCAAAG ATATGGAGTC CTGGATGAAA 
GTGATGTCGG ATGCGGCGAT GGCTCACAGC GAGCCAATCA GAAGGCTTGA GAAGGTAAAG 
CTGGACTCTC GCGTTCCTCA GGAGATGAAC AACATGCTGA ACCACCGCGT CTTGACACGA 
CCCGAGATCC AAAACAACGA GAGGAACCGC GAGACGCTCC GCCAAGAGGA CAAGAAGAGC 
AAACCATCAG AGAAACCTCA GAAAGACAAA GACAGAGTCA GCATGCAGAA AGACGGAGAG 
CGCTTCAGTG AAAACTACAC TCACCGGCAG AAAGACGGCG AGCGCTACAT GCTGCAGAAA 
GACCTGGAGA AATTTGTCCT GCAGAAAGAC GGAGAGAAGT ACGCCGTGCA GAAAGAAGGC 
GACAAATATC TCCTGCAGAA AGACGGACAC AAATACACAG TGCACAAAGA CGGAGACAAA 
TACACGCTTC AGAGAGAACT CGAGATGTAC AATCCACCAA AAGACGCGGA GAAGATCCCG 
CAGAAAGATG CAAGGTTTAG TCTGGTGCAG AAGGAGCTGG AGAAACCCGT CCCGCCACCG 
CGATCTGAGG AAAAATACGG CTTTCAGAAA GAGGGCACCA TTGAGCGTCC GCTAACTAAA 
ATAAATAGCA TTAAACTGCA GCCGGCTCAA GCAGCCGCCA TCGCTGCAGC CGTTTCATCC 
TCTAGGATGA TGCAGAAACA GGTTAATGGC TCCGGGGAAC GAGCGGGAGA CAGAAGTCCT 
GGAGATTTGA GTACTGTGAG GACGAGCGTC CCGCCACCAA CGCAGCCGGC TCCAGAACCA 
GAGCGAAGCC TCAGCAGAAC CAGCTCAATG CAGCAGCTGG AGCAGTGGGT TCGCACACAC 
CGCACACGAG CTCCAGACGA CGACACCAGG AGCATTACGT CCTACCAGAC CCTGCCAAGG 
AACATGCCGA GCCACCGGGC GCAGATTGTC CCTCGCTACC CTGAGGGCTA CCGCACTCTG 
CCCCGAAACA TGCTGCGTCC GGACAGCATC TGCAGTGTAG CAGGATCAGT GTACGACCGA 
GCGCTTCAGC CCAGCACGCA GGAAAAGCGG CGCTCCATCC GAGATGACAC CATGTGGCAG 
CTGTACGAGT GGCAGCAGCG GCAGGCGCAC AGTCGGCTTG GGTACGGGAC GCTGCCCAGT 
CCCAAAACTA TGGGGCAGAT CGCGGAGTCC ATCCCCACGT CCCCATCCCA TGGCTCTCTG 
GCCGGATATC ACACCTTCTC CCCGAACAGA CCGCTCAACC CTGACTCGCG CTCGGAGCTG 
TCTTCGCCTG TGTTTCGTGG AGACGTGACC ATCGAGCGAC GCCACCGACC ACACCTGTCC 
AAGTACTCCT ATCCTGCGGA ACGCAGAGCT GCTCCTCCCG CACAGAACAT CACTGCACAG 
TCTCTTCAAG GCAAAACGCC AGAGGAACTG ACCCTGCTGC TCATCAAACT GCGGCGGCAG 
CAGGCCGAGC TCAACAGCCT CCGTGAGCAC ACACTCGCGC AGCTCATGCA GCTGAACCTC 
GACGCCACCA ACCCAAAGAG TGAAATCCTC TCTCATCACC TGCAGAGGAA TCTCATGTAC 
TTGGACAGTC AGATGAAGGA GAACGAACCC ATAATCTTCA TGATTCACAC TATGATTGAG 
AACTCGGCGC CCCGGCCGCA GCTCTACCAG CAACAGATGA GTCCTGAAGA GTACAGAGAA 
AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT 
GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG 
CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC 
CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC 
ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT 
CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC 
GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG 
AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC 
AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC 
TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG 
CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC 
CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT 
GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG 
CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG 
GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC 
TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC 
GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG 
CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT 
GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA 
CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT 
GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT 
AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC 
TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG 
GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG 
ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG 
AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA 
CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG 
AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG 
TTCAACAGCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475077.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330752.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa142020
Clone ID Related Accession (Same CDS sequence)	XM_021475077.1
Accession Version	XM_021475077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG 
CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG 
CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG 
CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC 
GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC 
CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT 
ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG 
CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG 
GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC 
CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT 
CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT 
CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC 
AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA 
GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG 
CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA 
TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA 
CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC 
AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA 
CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC 
CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG 
ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA 
CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA 
CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG 
TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA 
CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT 
GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG 
CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT 
GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT 
CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330752.1
CDS	42..4238
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475077.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475077.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG 
CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG 
CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG 
CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC 
GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC 
CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT 
ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG 
CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG 
GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC 
CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT 
CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT 
CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC 
AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA 
GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG 
CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA 
TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA 
CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC 
AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA 
CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC 
CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG 
ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA 
CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA 
CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG 
TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA 
CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT 
GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG 
CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT 
GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT 
CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475078.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330753.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa142021
Clone ID Related Accession (Same CDS sequence)	XM_021475078.1
Accession Version	XM_021475078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAAATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT 
CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA 
CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC 
GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA 
GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG 
CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA 
CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT 
GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT 
GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA 
CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG 
TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC 
TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC 
ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC 
ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT 
CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG 
GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG 
GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG 
GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGC ATCATGTGAC 
AGGAAGAACA CATCATCACC ATCGTCATCA TCAGACTGGA AGTCTCGCCC CCTCCTTCAT 
CAGCTGCCCA ACACAAACAC AACAGCGTCC AGGCCTCAGT TCAACAGCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330753.1
CDS	42..4235
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475078.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475078.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAAATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT 
CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA 
CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC 
GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA 
GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG 
CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA 
CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT 
GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT 
GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA 
CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG 
TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC 
TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC 
ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC 
ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT 
CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG 
GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG 
GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG 
GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGC ATCATGTGAC 
AGGAAGAACA CATCATCACC ATCGTCATCA TCAGACTGGA AGTCTCGCCC CCTCCTTCAT 
CAGCTGCCCA ACACAAACAC AACAGCGTCC AGGCCTCAGT TCAACAGCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475079.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330754.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X3, mRNA.

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CloneID	ODa142022
Clone ID Related Accession (Same CDS sequence)	XM_021475079.1
Accession Version	XM_021475079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG 
CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG 
CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG 
CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC 
GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC 
CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT 
ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG 
CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG 
GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC 
CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT 
CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT 
CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC 
AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA 
GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG 
CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA 
TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA 
CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC 
AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA 
CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC 
CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG 
ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA 
CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA 
CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG 
TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA 
CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT 
GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG 
CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGTGAACAGT 
GTGTCGACTC CTCCTCAATC TCCAAGCTCC TCCTCCTCTC CTCCGCCTCC ACCCTCCCCT 
CCACAGCTGA CGGACGGGTC ACACTTCATG TGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330754.1
CDS	42..4220
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISVNSVSTPPQSPSSSSSPPPPPSPPQLTDGSHFMCV

Target ORF information:

RefSeq Version	XM_021475079.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475079.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG 
CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG 
CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG 
CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC 
GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC 
CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT 
ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG 
CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG 
GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC 
CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT 
CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT 
CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC 
AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA 
GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG 
CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA 
TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA 
CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC 
AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA 
CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC 
CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG 
ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA 
CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA 
CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG 
TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA 
CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT 
GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG 
CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGTGAACAGT 
GTGTCGACTC CTCCTCAATC TCCAAGCTCC TCCTCCTCTC CTCCGCCTCC ACCCTCCCCT 
CCACAGCTGA CGGACGGGTC ACACTTCATG TGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475080.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330755.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa142023
Clone ID Related Accession (Same CDS sequence)	XM_021475080.1
Accession Version	XM_021475080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGCAGATG 
AGTCCTGAAG AGTACAGAGA AAACACTTAC ATGTACAGGC CGGAGGAGCT CGATGTCGAC 
GCGAAGTTAA GTCGGCTCTG TGAGCAGGAC AAAGTAGTGC GAACTCAAGA AGAGAAACTG 
CAGCAGCTCC ACAGAGAAAA GCACACACTG GAGACGGCGC TGCTCTCCGC CAGTCAGGAG 
ATCGAGATGA GCTCTGAAAA CCCAGCGGCG GTCCAGAGCG TGGTCCAGCA GAGGGACGTC 
CTGCAGAGTG GCCTGCTGAG CACCTGCAGA GAGCTGAGCC GCGTCAGCAC TGAGCTGGAG 
CGAAGCTGGA GAGAATATGA TCGACTAGAG GCCGATGTTA CACTTGCCAA AACTAACCTG 
CTGGAGCAGC TGGAGGCCCT CGGCAGCCCA CAGACGGAGC CGCCCAGTCA AAAGCATGTT 
CAGATCCAGA AAGAGTTGTG GAGAATTCAG GATGTGATGG AGGCGCTGAG CAAGAACAAA 
CCCAAGAGAA ACCCTGAACC CAGTTTTCCT GGTGCAAACC CTCTGTCAAC CCTGCATAAA 
AGTGAAGACT CTGTAGACTC CTCGTGTGTT CCTCCTCGTC CTCCTCTGCC GTACTCATAT 
GAAGGAGGCG ATCGACCACC GCCCCTCCCC AACCCCGGTC AACCGCACAC CACCCGCACA 
ACACCACACC GACCAGAGGA CCGCAAAACT GCCCAACGCA ACGGCACGCA CACCGGCCCG 
GATTACAGGC TGTATAAGAG TGAGCCGGAG CTGACCACAG TAGCCGAAGT CGACGAGAGT 
AACGGTGAAG ACAAATCAGA GCAGACAGCA GAGAAAGAGC CGCCCGGCGT CACTAAAGGT 
ATAGTTTACC CGGTGGGAGT GGTCAGTCCC AGAACTAAAT CACCCATGCC CGAGTCCTCC 
ACCATCGCCT CCTACGTCAC CTTGAGGAAG ACCAAAAAAC CTGATCCCAG AACATGTCAG 
CCGCAGGACC GTCCGCGCAG CGCTGTGGAG CAGATGAGCA GTGGTGCTGA AGTGGGCCGA 
ACACGCATGA GTGTGGAGGA GCAGATGGAG AGAATCAGAC GACACCAGCA GGGGGCGCTG 
AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA TCCCGCAGCC TGTCCTTCAC TAAGGACAGC 
ACATACTACA CACTGCAGGC ACGTAAGAAG AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC 
GAGCGCAGAG ACATGACGCC TGTGGAGCGA GACACAAAAC ACACAAAACA CCCTGACGCA 
GAGGAAAACT GCAGAGACAC TAAGGAGCAG CTGCCAGCAC ACACCCACCT GGAGATTAAG 
GACAAAGCAT CTGTGACTCG CTCGGTCTCC ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT 
CCCAAACCCG TGCTGAAGCA GGACGAATCT GAGTGTAAAC TGGAGTACGA CAGAAAGCTG 
GCCACTCAGA ACAGCCTGCA GACGGCAGTG ATGGTGCGTG TGGGCACTGA GGAGGATGAA 
GAGGATGAAG AAGAGGAGCA GAAAGACTCA GTTCAGCAGC AGGAGGACTC TTATGAGCTC 
AGCAACAGCA AACACAGCAC ACTGATCTCA GCATCATGTG ACAGGAAGAA CACATCATCA 
CCATCGTCAT CATCAGACTG GAAGTCTCGC CCCCTCCTTC ATCAGCTGCC CAACACAAAC 
ACAACAGCGT CCAGGCCTCA GTTCAACAGC GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330755.1
CDS	42..4157
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475080.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475080.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGCAGATG 
AGTCCTGAAG AGTACAGAGA AAACACTTAC ATGTACAGGC CGGAGGAGCT CGATGTCGAC 
GCGAAGTTAA GTCGGCTCTG TGAGCAGGAC AAAGTAGTGC GAACTCAAGA AGAGAAACTG 
CAGCAGCTCC ACAGAGAAAA GCACACACTG GAGACGGCGC TGCTCTCCGC CAGTCAGGAG 
ATCGAGATGA GCTCTGAAAA CCCAGCGGCG GTCCAGAGCG TGGTCCAGCA GAGGGACGTC 
CTGCAGAGTG GCCTGCTGAG CACCTGCAGA GAGCTGAGCC GCGTCAGCAC TGAGCTGGAG 
CGAAGCTGGA GAGAATATGA TCGACTAGAG GCCGATGTTA CACTTGCCAA AACTAACCTG 
CTGGAGCAGC TGGAGGCCCT CGGCAGCCCA CAGACGGAGC CGCCCAGTCA AAAGCATGTT 
CAGATCCAGA AAGAGTTGTG GAGAATTCAG GATGTGATGG AGGCGCTGAG CAAGAACAAA 
CCCAAGAGAA ACCCTGAACC CAGTTTTCCT GGTGCAAACC CTCTGTCAAC CCTGCATAAA 
AGTGAAGACT CTGTAGACTC CTCGTGTGTT CCTCCTCGTC CTCCTCTGCC GTACTCATAT 
GAAGGAGGCG ATCGACCACC GCCCCTCCCC AACCCCGGTC AACCGCACAC CACCCGCACA 
ACACCACACC GACCAGAGGA CCGCAAAACT GCCCAACGCA ACGGCACGCA CACCGGCCCG 
GATTACAGGC TGTATAAGAG TGAGCCGGAG CTGACCACAG TAGCCGAAGT CGACGAGAGT 
AACGGTGAAG ACAAATCAGA GCAGACAGCA GAGAAAGAGC CGCCCGGCGT CACTAAAGGT 
ATAGTTTACC CGGTGGGAGT GGTCAGTCCC AGAACTAAAT CACCCATGCC CGAGTCCTCC 
ACCATCGCCT CCTACGTCAC CTTGAGGAAG ACCAAAAAAC CTGATCCCAG AACATGTCAG 
CCGCAGGACC GTCCGCGCAG CGCTGTGGAG CAGATGAGCA GTGGTGCTGA AGTGGGCCGA 
ACACGCATGA GTGTGGAGGA GCAGATGGAG AGAATCAGAC GACACCAGCA GGGGGCGCTG 
AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA TCCCGCAGCC TGTCCTTCAC TAAGGACAGC 
ACATACTACA CACTGCAGGC ACGTAAGAAG AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC 
GAGCGCAGAG ACATGACGCC TGTGGAGCGA GACACAAAAC ACACAAAACA CCCTGACGCA 
GAGGAAAACT GCAGAGACAC TAAGGAGCAG CTGCCAGCAC ACACCCACCT GGAGATTAAG 
GACAAAGCAT CTGTGACTCG CTCGGTCTCC ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT 
CCCAAACCCG TGCTGAAGCA GGACGAATCT GAGTGTAAAC TGGAGTACGA CAGAAAGCTG 
GCCACTCAGA ACAGCCTGCA GACGGCAGTG ATGGTGCGTG TGGGCACTGA GGAGGATGAA 
GAGGATGAAG AAGAGGAGCA GAAAGACTCA GTTCAGCAGC AGGAGGACTC TTATGAGCTC 
AGCAACAGCA AACACAGCAC ACTGATCTCA GCATCATGTG ACAGGAAGAA CACATCATCA 
CCATCGTCAT CATCAGACTG GAAGTCTCGC CCCCTCCTTC ATCAGCTGCC CAACACAAAC 
ACAACAGCGT CCAGGCCTCA GTTCAACAGC GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475081.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330756.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa142024
Clone ID Related Accession (Same CDS sequence)	XM_021475081.1
Accession Version	XM_021475081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT 
CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG 
AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG 
CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC 
GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG 
CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA 
AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG 
GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG 
ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC 
AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT 
GAAGACTCTG TAGACTCCTC GTGTGTTCCT CCTCGTCCTC CTCTGCCGTA CTCATATGAA 
GGAGGCGATC GACCACCGCC CCTCCCCAAC CCCGGTCAAC CGCACACCAC CCGCACAACA 
CCACACCGAC CAGAGGACCG CAAAACTGCC CAACGCAACG GCACGCACAC CGGCCCGGAT 
TACAGGCTGT ATAAGAGTGA GCCGGAGCTG ACCACAGTAG CCGAAGTCGA CGAGAGTAAC 
GGTGAAGACA AATCAGAGCA GACAGCAGAG AAAGAGCCGC CCGGCGTCAC TAAAGGTATA 
GTTTACCCGG TGGGAGTGGT CAGTCCCAGA ACTAAATCAC CCATGCCCGA GTCCTCCACC 
ATCGCCTCCT ACGTCACCTT GAGGAAGACC AAAAAACCTG ATCCCAGAAC ATGTCAGCCG 
CAGGACCGTC CGCGCAGCGC TGTGGAGCAG ATGAGCAGTG GTGCTGAAGT GGGCCGAACA 
CGCATGAGTG TGGAGGAGCA GATGGAGAGA ATCAGACGAC ACCAGCAGGG GGCGCTGAGA 
GAGCGCAGGA GAGAAGATGG CTCTCTATCC CGCAGCCTGT CCTTCACTAA GGACAGCACA 
TACTACACAC TGCAGGCACG TAAGAAGAGT CCGGTGATCT CTCCAGACGA GCAGGACGAG 
CGCAGAGACA TGACGCCTGT GGAGCGAGAC ACAAAACACA CAAAACACCC TGACGCAGAG 
GAAAACTGCA GAGACACTAA GGAGCAGCTG CCAGCACACA CCCACCTGGA GATTAAGGAC 
AAAGCATCTG TGACTCGCTC GGTCTCCATG AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC 
AAACCCGTGC TGAAGCAGGA CGAATCTGAG TGTAAACTGG AGTACGACAG AAAGCTGGCC 
ACTCAGAACA GCCTGCAGAC GGCAGTGATG GTGCGTGTGG GCACTGAGGA GGATGAAGAG 
GATGAAGAAG AGGAGCAGAA AGACTCAGTT CAGCAGCAGG AGGACTCTTA TGAGCTCAGC 
AACAGCAAAC ACAGCACACT GATCTCAGCA TCATGTGACA GGAAGAACAC ATCATCACCA 
TCGTCATCAT CAGACTGGAA GTCTCGCCCC CTCCTTCATC AGCTGCCCAA CACAAACACA 
ACAGCGTCCA GGCCTCAGTT CAACAGCGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330756.1
CDS	42..4154
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475081.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475081.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT 
CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG 
AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG 
CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC 
GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG 
CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA 
AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG 
GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG 
ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC 
AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT 
GAAGACTCTG TAGACTCCTC GTGTGTTCCT CCTCGTCCTC CTCTGCCGTA CTCATATGAA 
GGAGGCGATC GACCACCGCC CCTCCCCAAC CCCGGTCAAC CGCACACCAC CCGCACAACA 
CCACACCGAC CAGAGGACCG CAAAACTGCC CAACGCAACG GCACGCACAC CGGCCCGGAT 
TACAGGCTGT ATAAGAGTGA GCCGGAGCTG ACCACAGTAG CCGAAGTCGA CGAGAGTAAC 
GGTGAAGACA AATCAGAGCA GACAGCAGAG AAAGAGCCGC CCGGCGTCAC TAAAGGTATA 
GTTTACCCGG TGGGAGTGGT CAGTCCCAGA ACTAAATCAC CCATGCCCGA GTCCTCCACC 
ATCGCCTCCT ACGTCACCTT GAGGAAGACC AAAAAACCTG ATCCCAGAAC ATGTCAGCCG 
CAGGACCGTC CGCGCAGCGC TGTGGAGCAG ATGAGCAGTG GTGCTGAAGT GGGCCGAACA 
CGCATGAGTG TGGAGGAGCA GATGGAGAGA ATCAGACGAC ACCAGCAGGG GGCGCTGAGA 
GAGCGCAGGA GAGAAGATGG CTCTCTATCC CGCAGCCTGT CCTTCACTAA GGACAGCACA 
TACTACACAC TGCAGGCACG TAAGAAGAGT CCGGTGATCT CTCCAGACGA GCAGGACGAG 
CGCAGAGACA TGACGCCTGT GGAGCGAGAC ACAAAACACA CAAAACACCC TGACGCAGAG 
GAAAACTGCA GAGACACTAA GGAGCAGCTG CCAGCACACA CCCACCTGGA GATTAAGGAC 
AAAGCATCTG TGACTCGCTC GGTCTCCATG AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC 
AAACCCGTGC TGAAGCAGGA CGAATCTGAG TGTAAACTGG AGTACGACAG AAAGCTGGCC 
ACTCAGAACA GCCTGCAGAC GGCAGTGATG GTGCGTGTGG GCACTGAGGA GGATGAAGAG 
GATGAAGAAG AGGAGCAGAA AGACTCAGTT CAGCAGCAGG AGGACTCTTA TGAGCTCAGC 
AACAGCAAAC ACAGCACACT GATCTCAGCA TCATGTGACA GGAAGAACAC ATCATCACCA 
TCGTCATCAT CAGACTGGAA GTCTCGCCCC CTCCTTCATC AGCTGCCCAA CACAAACACA 
ACAGCGTCCA GGCCTCAGTT CAACAGCGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475082.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330757.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X7, mRNA.

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CloneID	ODa142025
Clone ID Related Accession (Same CDS sequence)	XM_021475082.1
Accession Version	XM_021475082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG 
CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG 
CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG 
CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC 
GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC 
CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT 
ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG 
CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG 
GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC 
CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT 
CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT 
CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC 
AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA 
GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG 
CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA 
TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA 
CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC 
AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA 
CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC 
CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG 
ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA 
CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA 
CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG 
TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA 
CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT 
GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG 
CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT 
GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT 
CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330757.1
CDS	42..4118
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475082.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475082.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG 
CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG 
CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG 
CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC 
GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC 
CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT 
ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG 
CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG 
GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC 
CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT 
CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT 
CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC 
AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA 
GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG 
CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA 
TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA 
CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC 
AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA 
CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC 
CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG 
ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA 
CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA 
CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG 
TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA 
CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT 
GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG 
CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT 
GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT 
CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475083.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330758.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X8, mRNA.

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CloneID	ODa142026
Clone ID Related Accession (Same CDS sequence)	XM_021475083.1
Accession Version	XM_021475083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG 
CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG 
CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG 
CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC 
GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC 
CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT 
ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG 
CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG 
GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC 
CCTCTGTCAA CCCTGCATAA AAGTGAAGGC CCGGATTACA GGCTGTATAA GAGTGAGCCG 
GAGCTGACCA CAGTAGCCGA AGTCGACGAG AGTAACGGTG AAGACAAATC AGAGCAGACA 
GCAGAGAAAG AGCCGCCCGG CGTCACTAAA GGTATAGTTT ACCCGGTGGG AGTGGTCAGT 
CCCAGAACTA AATCACCCAT GCCCGAGTCC TCCACCATCG CCTCCTACGT CACCTTGAGG 
AAGACCAAAA AACCTGATCC CAGAACATGT CAGCCGCAGG ACCGTCCGCG CAGCGCTGTG 
GAGCAGATGA GCAGTGGTGC TGAAGTGGGC CGAACACGCA TGAGTGTGGA GGAGCAGATG 
GAGAGAATCA GACGACACCA GCAGGGGGCG CTGAGAGAGC GCAGGAGAGA AGATGGCTCT 
CTATCCCGCA GCCTGTCCTT CACTAAGGAC AGCACATACT ACACACTGCA GGCACGTAAG 
AAGAGTCCGG TGATCTCTCC AGACGAGCAG GACGAGCGCA GAGACATGAC GCCTGTGGAG 
CGAGACACAA AACACACAAA ACACCCTGAC GCAGAGGAAA ACTGCAGAGA CACTAAGGAG 
CAGCTGCCAG CACACACCCA CCTGGAGATT AAGGACAAAG CATCTGTGAC TCGCTCGGTC 
TCCATGAAAG AGTCTCTCCT GAAGAGTGTG AGTCCCAAAC CCGTGCTGAA GCAGGACGAA 
TCTGAGTGTA AACTGGAGTA CGACAGAAAG CTGGCCACTC AGAACAGCCT GCAGACGGCA 
GTGATGGTGC GTGTGGGCAC TGAGGAGGAT GAAGAGGATG AAGAAGAGGA GCAGAAAGAC 
TCAGTTCAGC AGCAGGAGGA CTCTTATGAG CTCAGCAACA GCAAACACAG CACACTGATC 
TCAGCATCAT GTGACAGGAA GAACACATCA TCACCATCGT CATCATCAGA CTGGAAGTCT 
CGCCCCCTCC TTCATCAGCT GCCCAACACA AACACAACAG CGTCCAGGCC TCAGTTCAAC 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330758.1
CDS	42..4070
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475083.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475083.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG 
CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG 
CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG 
CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC 
GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC 
CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT 
ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG 
CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG 
GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC 
CCTCTGTCAA CCCTGCATAA AAGTGAAGGC CCGGATTACA GGCTGTATAA GAGTGAGCCG 
GAGCTGACCA CAGTAGCCGA AGTCGACGAG AGTAACGGTG AAGACAAATC AGAGCAGACA 
GCAGAGAAAG AGCCGCCCGG CGTCACTAAA GGTATAGTTT ACCCGGTGGG AGTGGTCAGT 
CCCAGAACTA AATCACCCAT GCCCGAGTCC TCCACCATCG CCTCCTACGT CACCTTGAGG 
AAGACCAAAA AACCTGATCC CAGAACATGT CAGCCGCAGG ACCGTCCGCG CAGCGCTGTG 
GAGCAGATGA GCAGTGGTGC TGAAGTGGGC CGAACACGCA TGAGTGTGGA GGAGCAGATG 
GAGAGAATCA GACGACACCA GCAGGGGGCG CTGAGAGAGC GCAGGAGAGA AGATGGCTCT 
CTATCCCGCA GCCTGTCCTT CACTAAGGAC AGCACATACT ACACACTGCA GGCACGTAAG 
AAGAGTCCGG TGATCTCTCC AGACGAGCAG GACGAGCGCA GAGACATGAC GCCTGTGGAG 
CGAGACACAA AACACACAAA ACACCCTGAC GCAGAGGAAA ACTGCAGAGA CACTAAGGAG 
CAGCTGCCAG CACACACCCA CCTGGAGATT AAGGACAAAG CATCTGTGAC TCGCTCGGTC 
TCCATGAAAG AGTCTCTCCT GAAGAGTGTG AGTCCCAAAC CCGTGCTGAA GCAGGACGAA 
TCTGAGTGTA AACTGGAGTA CGACAGAAAG CTGGCCACTC AGAACAGCCT GCAGACGGCA 
GTGATGGTGC GTGTGGGCAC TGAGGAGGAT GAAGAGGATG AAGAAGAGGA GCAGAAAGAC 
TCAGTTCAGC AGCAGGAGGA CTCTTATGAG CTCAGCAACA GCAAACACAG CACACTGATC 
TCAGCATCAT GTGACAGGAA GAACACATCA TCACCATCGT CATCATCAGA CTGGAAGTCT 
CGCCCCCTCC TTCATCAGCT GCCCAACACA AACACAACAG CGTCCAGGCC TCAGTTCAAC 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475084.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330759.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa142027
Clone ID Related Accession (Same CDS sequence)	XM_021475084.1
Accession Version	XM_021475084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACACCTG 
AATTGTGGGA ATCTTGTCCA TCCTGTTTTA AGCAGACCAG CGACCAAAGC ACCAGAGGCG 
GAGAAGAAGG AGCGTCCCAC CAGCACCATG AGCGAAGCCT CAAACTACAC CGGCGGCTCC 
GACTACACCA CACATCCCAG CAGCCCCACA ACCAGACCAT CCCGGTCCTC AAAGAAAGTG 
CACAACTTTG GGAAAAGGTC CAACTCCATC AAGAGGAACC CCAATGCACC TGTGATCAAA 
AACGGCTGGC TTCACAAACA GGACAGCACT GGGATGAAGA TGTGGAAGAA GAGATGGTTC 
GTTCTGTCTG ATATGTGCCT CTTCTACTAC AGAGATGAGA AGGAAGAGGG GATTCTGGGA 
AGCATCCTCC TGCCTAGCTT TCACATCTCC ATGCTGTCCG TGGACGACCA TATTACCCGG 
AAATACGCCT TCAAGGCGAC GCATCCAAAC ATGCGGACGT ATTATTTCAG CACAGACACA 
GCCAAAGATA TGGAGTCCTG GATGAAAGTG ATGTCGGATG CGGCGATGGC TCACAGCGAG 
CCAATCAGAA GGCTTGAGAA GGTAAAGCTG GACTCTCGCG TTCCTCAGGA GATGAACAAC 
ATGCTGAACC ACCGCGTCTT GACACGACCC GAGATCCAAA ACAACGAGAG GAACCGCGAG 
ACGCTCCGCC AAGAGGACAA GAAGAGCAAA CCATCAGAGA AACCTCAGAA AGACAAAGAC 
AGAGTCAGCA TGCAGAAAGA CGGAGAGCGC TTCAGTGAAA ACTACACTCA CCGGCAGAAA 
GACGGCGAGC GCTACATGCT GCAGAAAGAC CTGGAGAAAT TTGTCCTGCA GAAAGACGGA 
GAGAAGTACG CCGTGCAGAA AGAAGGCGAC AAATATCTCC TGCAGAAAGA CGGACACAAA 
TACACAGTGC ACAAAGACGG AGACAAATAC ACGCTTCAGA GAGAACTCGA GATGTACAAT 
CCACCAAAAG ACGCGGAGAA GATCCCGCAG AAAGATGCAA GGTTTAGTCT GGTGCAGAAG 
GAGCTGGAGA AACCCGTCCC GCCACCGCGA TCTGAGGAAA AATACGGCTT TCAGAAAGAG 
GGCACCATTG AGCGTCCGCT AACTAAAATA AATAGCATTA AACTGCAGCC GGCTCAAGCA 
GCCGCCATCG CTGCAGCCGT TTCATCCTCT AGGATGATGC AGAAACAGGT TAATGGCTCC 
GGGGAACGAG CGGGAGACAG AAGTCCTGGA GATTTGAGTA CTGTGAGGAC GAGCGTCCCG 
CCACCAACGC AGCCGGCTCC AGAACCAGAG CGAAGCCTCA GCAGAACCAG CTCAATGCAG 
CAGCTGGAGC AGTGGGTTCG CACACACCGC ACACGAGCTC CAGACGACGA CACCAGGAGC 
ATTACGTCCT ACCAGACCCT GCCAAGGAAC ATGCCGAGCC ACCGGGCGCA GATTGTCCCT 
CGCTACCCTG AGGGCTACCG CACTCTGCCC CGAAACATGC TGCGTCCGGA CAGCATCTGC 
AGTGTAGCAG GATCAGTGTA CGACCGAGCG CTTCAGCCCA GCACGCAGGA AAAGCGGCGC 
TCCATCCGAG ATGACACCAT GTGGCAGCTG TACGAGTGGC AGCAGCGGCA GGCGCACAGT 
CGGCTTGGGT ACGGGACGCT GCCCAGTCCC AAAACTATGG GGCAGATCGC GGAGTCCATC 
CCCACGTCCC CATCCCATGG CTCTCTGGCC GGATATCACA CCTTCTCCCC GAACAGACCG 
CTCAACCCTG ACTCGCGCTC GGAGCTGTCT TCGCCTGTGT TTCGTGGAGA CGTGACCATC 
GAGCGACGCC ACCGACCACA CCTGTCCAAG TACTCCTATC CTGCGGAACG CAGAGCTGCT 
CCTCCCGCAC AGAACATCAC TGCACAGTCT CTTCAAGGCA AAACGCCAGA GGAACTGACC 
CTGCTGCTCA TCAAACTGCG GCGGCAGCAG GCCGAGCTCA ACAGCCTCCG TGAGCACACA 
CTCGCGCAGC TCATGCAGCT GAACCTCGAC GCCACCAACC CAAAGAGTGA AATCCTCTCT 
CATCACCTGC AGAGGAATCT CATGTACTTG GACAGTCAGA TGAAGGAGAA CGAACCCATA 
ATCTTCATGA TTCACACTAT GATTGAGAAC TCGGCGCCCC GGCCGCAGCT CTACCAGCAA 
CAGATGAGTC CTGAAGAGTA CAGAGAAAAC ACTTACATGT ACAGGCCGGA GGAGCTCGAT 
GTCGACGCGA AGTTAAGTCG GCTCTGTGAG CAGGACAAAG TAGTGCGAAC TCAAGAAGAG 
AAACTGCAGC AGCTCCACAG AGAAAAGCAC ACACTGGAGA CGGCGCTGCT CTCCGCCAGT 
CAGGAGATCG AGATGAGCTC TGAAAACCCA GCGGCGGTCC AGAGCGTGGT CCAGCAGAGG 
GACGTCCTGC AGAGTGGCCT GCTGAGCACC TGCAGAGAGC TGAGCCGCGT CAGCACTGAG 
CTGGAGCGAA GCTGGAGAGA ATATGATCGA CTAGAGGCCG ATGTTACACT TGCCAAAACT 
AACCTGCTGG AGCAGCTGGA GGCCCTCGGC AGCCCACAGA CGGAGCCGCC CAGTCAAAAG 
CATGTTCAGA TCCAGAAAGA GTTGTGGAGA ATTCAGGATG TGATGGAGGC GCTGAGCAAG 
AACAAACCCA AGAGAAACCC TGAACCCAGT TTTCCTGGTG CAAACCCTCT GTCAACCCTG 
CATAAAAGTG AAGACTCTGT AGACTCCTCG TGTGTTCCTC CTCGTCCTCC TCTGCCGTAC 
TCATATGAAG GAGGCGATCG ACCACCGCCC CTCCCCAACC CCGGTCAACC GCACACCACC 
CGCACAACAC CACACCGACC AGAGGACCGC AAAACTGCCC AACGCAACGG CACGCACACC 
GGCCCGGATT ACAGGCTGTA TAAGAGTGAG CCGGAGCTGA CCACAGTAGC CGAAGTCGAC 
GAGAGTAACG GTGAAGACAA ATCAGAGCAG ACAGCAGAGA AAGAGCCGCC CGGCGTCACT 
AAAGGTATAG TTTACCCGGT GGGAGTGGTC AGTCCCAGAA CTAAATCACC CATGCCCGAG 
TCCTCCACCA TCGCCTCCTA CGTCACCTTG AGGAAGACCA AAAAACCTGA TCCCAGAACA 
TGTCAGCCGC AGGACCGTCC GCGCAGCGCT GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG 
GGCCGAACAC GCATGAGTGT GGAGGAGCAG ATGGAGAGAA TCAGACGACA CCAGCAGGGG 
GCGCTGAGAG AGCGCAGGAG AGAAGATGGC TCTCTATCCC GCAGCCTGTC CTTCACTAAG 
GACAGCACAT ACTACACACT GCAGGCACGT AAGAAGAGTC CGGTGATCTC TCCAGACGAG 
CAGGACGAGC GCAGAGACAT GACGCCTGTG GAGCGAGACA CAAAACACAC AAAACACCCT 
GACGCAGAGG AAAACTGCAG AGACACTAAG GAGCAGCTGC CAGCACACAC CCACCTGGAG 
ATTAAGGACA AAGCATCTGT GACTCGCTCG GTCTCCATGA AAGAGTCTCT CCTGAAGAGT 
GTGAGTCCCA AACCCGTGCT GAAGCAGGAC GAATCTGAGT GTAAACTGGA GTACGACAGA 
AAGCTGGCCA CTCAGAACAG CCTGCAGACG GCAGTGATGG TGCGTGTGGG CACTGAGGAG 
GATGAAGAGG ATGAAGAAGA GGAGCAGAAA GACTCAGTTC AGCAGCAGGA GGACTCTTAT 
GAGCTCAGCA ACAGCAAACA CAGCACACTG ATCTCAGCAT CATGTGACAG GAAGAACACA 
TCATCACCAT CGTCATCATC AGACTGGAAG TCTCGCCCCC TCCTTCATCA GCTGCCCAAC 
ACAAACACAA CAGCGTCCAG GCCTCAGTTC AACAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330759.1
CDS	75..4076
Translation	MLKFCTTSRSSHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475084.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475084.1

ORF Insert Sequence:

ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACACCTG 
AATTGTGGGA ATCTTGTCCA TCCTGTTTTA AGCAGACCAG CGACCAAAGC ACCAGAGGCG 
GAGAAGAAGG AGCGTCCCAC CAGCACCATG AGCGAAGCCT CAAACTACAC CGGCGGCTCC 
GACTACACCA CACATCCCAG CAGCCCCACA ACCAGACCAT CCCGGTCCTC AAAGAAAGTG 
CACAACTTTG GGAAAAGGTC CAACTCCATC AAGAGGAACC CCAATGCACC TGTGATCAAA 
AACGGCTGGC TTCACAAACA GGACAGCACT GGGATGAAGA TGTGGAAGAA GAGATGGTTC 
GTTCTGTCTG ATATGTGCCT CTTCTACTAC AGAGATGAGA AGGAAGAGGG GATTCTGGGA 
AGCATCCTCC TGCCTAGCTT TCACATCTCC ATGCTGTCCG TGGACGACCA TATTACCCGG 
AAATACGCCT TCAAGGCGAC GCATCCAAAC ATGCGGACGT ATTATTTCAG CACAGACACA 
GCCAAAGATA TGGAGTCCTG GATGAAAGTG ATGTCGGATG CGGCGATGGC TCACAGCGAG 
CCAATCAGAA GGCTTGAGAA GGTAAAGCTG GACTCTCGCG TTCCTCAGGA GATGAACAAC 
ATGCTGAACC ACCGCGTCTT GACACGACCC GAGATCCAAA ACAACGAGAG GAACCGCGAG 
ACGCTCCGCC AAGAGGACAA GAAGAGCAAA CCATCAGAGA AACCTCAGAA AGACAAAGAC 
AGAGTCAGCA TGCAGAAAGA CGGAGAGCGC TTCAGTGAAA ACTACACTCA CCGGCAGAAA 
GACGGCGAGC GCTACATGCT GCAGAAAGAC CTGGAGAAAT TTGTCCTGCA GAAAGACGGA 
GAGAAGTACG CCGTGCAGAA AGAAGGCGAC AAATATCTCC TGCAGAAAGA CGGACACAAA 
TACACAGTGC ACAAAGACGG AGACAAATAC ACGCTTCAGA GAGAACTCGA GATGTACAAT 
CCACCAAAAG ACGCGGAGAA GATCCCGCAG AAAGATGCAA GGTTTAGTCT GGTGCAGAAG 
GAGCTGGAGA AACCCGTCCC GCCACCGCGA TCTGAGGAAA AATACGGCTT TCAGAAAGAG 
GGCACCATTG AGCGTCCGCT AACTAAAATA AATAGCATTA AACTGCAGCC GGCTCAAGCA 
GCCGCCATCG CTGCAGCCGT TTCATCCTCT AGGATGATGC AGAAACAGGT TAATGGCTCC 
GGGGAACGAG CGGGAGACAG AAGTCCTGGA GATTTGAGTA CTGTGAGGAC GAGCGTCCCG 
CCACCAACGC AGCCGGCTCC AGAACCAGAG CGAAGCCTCA GCAGAACCAG CTCAATGCAG 
CAGCTGGAGC AGTGGGTTCG CACACACCGC ACACGAGCTC CAGACGACGA CACCAGGAGC 
ATTACGTCCT ACCAGACCCT GCCAAGGAAC ATGCCGAGCC ACCGGGCGCA GATTGTCCCT 
CGCTACCCTG AGGGCTACCG CACTCTGCCC CGAAACATGC TGCGTCCGGA CAGCATCTGC 
AGTGTAGCAG GATCAGTGTA CGACCGAGCG CTTCAGCCCA GCACGCAGGA AAAGCGGCGC 
TCCATCCGAG ATGACACCAT GTGGCAGCTG TACGAGTGGC AGCAGCGGCA GGCGCACAGT 
CGGCTTGGGT ACGGGACGCT GCCCAGTCCC AAAACTATGG GGCAGATCGC GGAGTCCATC 
CCCACGTCCC CATCCCATGG CTCTCTGGCC GGATATCACA CCTTCTCCCC GAACAGACCG 
CTCAACCCTG ACTCGCGCTC GGAGCTGTCT TCGCCTGTGT TTCGTGGAGA CGTGACCATC 
GAGCGACGCC ACCGACCACA CCTGTCCAAG TACTCCTATC CTGCGGAACG CAGAGCTGCT 
CCTCCCGCAC AGAACATCAC TGCACAGTCT CTTCAAGGCA AAACGCCAGA GGAACTGACC 
CTGCTGCTCA TCAAACTGCG GCGGCAGCAG GCCGAGCTCA ACAGCCTCCG TGAGCACACA 
CTCGCGCAGC TCATGCAGCT GAACCTCGAC GCCACCAACC CAAAGAGTGA AATCCTCTCT 
CATCACCTGC AGAGGAATCT CATGTACTTG GACAGTCAGA TGAAGGAGAA CGAACCCATA 
ATCTTCATGA TTCACACTAT GATTGAGAAC TCGGCGCCCC GGCCGCAGCT CTACCAGCAA 
CAGATGAGTC CTGAAGAGTA CAGAGAAAAC ACTTACATGT ACAGGCCGGA GGAGCTCGAT 
GTCGACGCGA AGTTAAGTCG GCTCTGTGAG CAGGACAAAG TAGTGCGAAC TCAAGAAGAG 
AAACTGCAGC AGCTCCACAG AGAAAAGCAC ACACTGGAGA CGGCGCTGCT CTCCGCCAGT 
CAGGAGATCG AGATGAGCTC TGAAAACCCA GCGGCGGTCC AGAGCGTGGT CCAGCAGAGG 
GACGTCCTGC AGAGTGGCCT GCTGAGCACC TGCAGAGAGC TGAGCCGCGT CAGCACTGAG 
CTGGAGCGAA GCTGGAGAGA ATATGATCGA CTAGAGGCCG ATGTTACACT TGCCAAAACT 
AACCTGCTGG AGCAGCTGGA GGCCCTCGGC AGCCCACAGA CGGAGCCGCC CAGTCAAAAG 
CATGTTCAGA TCCAGAAAGA GTTGTGGAGA ATTCAGGATG TGATGGAGGC GCTGAGCAAG 
AACAAACCCA AGAGAAACCC TGAACCCAGT TTTCCTGGTG CAAACCCTCT GTCAACCCTG 
CATAAAAGTG AAGACTCTGT AGACTCCTCG TGTGTTCCTC CTCGTCCTCC TCTGCCGTAC 
TCATATGAAG GAGGCGATCG ACCACCGCCC CTCCCCAACC CCGGTCAACC GCACACCACC 
CGCACAACAC CACACCGACC AGAGGACCGC AAAACTGCCC AACGCAACGG CACGCACACC 
GGCCCGGATT ACAGGCTGTA TAAGAGTGAG CCGGAGCTGA CCACAGTAGC CGAAGTCGAC 
GAGAGTAACG GTGAAGACAA ATCAGAGCAG ACAGCAGAGA AAGAGCCGCC CGGCGTCACT 
AAAGGTATAG TTTACCCGGT GGGAGTGGTC AGTCCCAGAA CTAAATCACC CATGCCCGAG 
TCCTCCACCA TCGCCTCCTA CGTCACCTTG AGGAAGACCA AAAAACCTGA TCCCAGAACA 
TGTCAGCCGC AGGACCGTCC GCGCAGCGCT GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG 
GGCCGAACAC GCATGAGTGT GGAGGAGCAG ATGGAGAGAA TCAGACGACA CCAGCAGGGG 
GCGCTGAGAG AGCGCAGGAG AGAAGATGGC TCTCTATCCC GCAGCCTGTC CTTCACTAAG 
GACAGCACAT ACTACACACT GCAGGCACGT AAGAAGAGTC CGGTGATCTC TCCAGACGAG 
CAGGACGAGC GCAGAGACAT GACGCCTGTG GAGCGAGACA CAAAACACAC AAAACACCCT 
GACGCAGAGG AAAACTGCAG AGACACTAAG GAGCAGCTGC CAGCACACAC CCACCTGGAG 
ATTAAGGACA AAGCATCTGT GACTCGCTCG GTCTCCATGA AAGAGTCTCT CCTGAAGAGT 
GTGAGTCCCA AACCCGTGCT GAAGCAGGAC GAATCTGAGT GTAAACTGGA GTACGACAGA 
AAGCTGGCCA CTCAGAACAG CCTGCAGACG GCAGTGATGG TGCGTGTGGG CACTGAGGAG 
GATGAAGAGG ATGAAGAAGA GGAGCAGAAA GACTCAGTTC AGCAGCAGGA GGACTCTTAT 
GAGCTCAGCA ACAGCAAACA CAGCACACTG ATCTCAGCAT CATGTGACAG GAAGAACACA 
TCATCACCAT CGTCATCATC AGACTGGAAG TCTCGCCCCC TCCTTCATCA GCTGCCCAAC 
ACAAACACAA CAGCGTCCAG GCCTCAGTTC AACAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475085.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330760.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa142028
Clone ID Related Accession (Same CDS sequence)	XM_021475085.1
Accession Version	XM_021475085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGCAGATG 
AGTCCTGAAG AGTACAGAGA AAACACTTAC ATGTACAGGC CGGAGGAGCT CGATGTCGAC 
GCGAAGTTAA GTCGGCTCTG TGAGCAGGAC AAAGTAGTGC GAACTCAAGA AGAGAAACTG 
CAGCAGCTCC ACAGAGAAAA GCACACACTG GAGACGGCGC TGCTCTCCGC CAGTCAGGAG 
ATCGAGATGA GCTCTGAAAA CCCAGCGGCG GTCCAGAGCG TGGTCCAGCA GAGGGACGTC 
CTGCAGAGTG GCCTGCTGAG CACCTGCAGA GAGCTGAGCC GCGTCAGCAC TGAGCTGGAG 
CGAAGCTGGA GAGAATATGA TCGACTAGAG GCCGATGTTA CACTTGCCAA AACTAACCTG 
CTGGAGCAGC TGGAGGCCCT CGGCAGCCCA CAGACGGAGC CGCCCAGTCA AAAGCATGTT 
CAGATCCAGA AAGAGTTGTG GAGAATTCAG GATGTGATGG AGGCGCTGAG CAAGAACAAA 
CCCAAGAGAA ACCCTGAACC CAGTTTTCCT GGTGCAAACC CTCTGTCAAC CCTGCATAAA 
AGTGAAGACT CTGTAGACTC CTCGTGTGTT CCTCCTCGTC CTCCTCTGCC GTACTCATAT 
GAAGGAGGCG ATCGACCACC GCCCCTCCCC AACCCCGGTC AACCGCACAC CACCCGCACA 
ACACCACACC GACCAGAGGA CCGCAAAACT GCCCAACGCA ACGGCACGCA CACCGGCCCG 
GATTACAGGC TGTATAAGAG TGAGCCGGAG CTGACCACAG TAGCCGAAGT CGACGAGAGT 
AACGGTGAAG ACAAATCAGA GCAGACAGCA GAGAAAGAGC CGCCCGGCGT CACTAAAGGT 
ATAGTTTACC CGGTGGGAGT GGTCAGTCCC AGAACTAAAT CACCCATGCC CGAGTCCTCC 
ACCATCGCCT CCTACGTCAC CTTGAGGAAG ACCAAAAAAC CTGATCCCAG AACATGTCAG 
CCGCAGGACC GTCCGCGCAG CGCTGTGGAG CAGATGAGCA GTGGTGCTGA AGTGGGCCGA 
ACACGCATGA GTGTGGAGGA GCAGATGGAG AGAATCAGAC GACACCAGCA GGGGGCGCTG 
AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA TCCCGCAGCC TGTCCTTCAC TAAGGACAGC 
ACATACTACA CACTGCAGGC ACGTAAGAAG AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC 
GAGCGCAGAG ACATGACGCC TGTGGAGCGA GACACAAAAC ACACAAAACA CCCTGACGCA 
GAGGAAAACT GCAGAGACAC TAAGGAGCAG CTGCCAGCAC ACACCCACCT GGAGATTAAG 
GACAAAGCAT CTGTGACTCG CTCGGTCTCC ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT 
CCCAAACCCG TGCTGAAGCA GGACGAATCT GAGTGTAAAC TGGAGTACGA CAGAAAGCTG 
GCCACTCAGA ACAGCCTGCA GACGGCAGTG ATGGTGCGTG TGGGCACTGA GGAGGATGAA 
GAGGATGAAG AAGAGGAGCA GAAAGACTCA GTTCAGCAGC AGGAGGACTC TTATGAGCTC 
AGCAACAGCA AACACAGCAC ACTGATCTCA GCATCATGTG ACAGGAAGAA CACATCATCA 
CCATCGTCAT CATCAGACTG GAAGTCTCGC CCCCTCCTTC ATCAGCTGCC CAACACAAAC 
ACAACAGCGT CCAGGCCTCA GTTCAACAGC GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330760.1
CDS	42..4037
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTSEILSHHLQRNLMYLDSQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475085.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475085.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGCAGATG 
AGTCCTGAAG AGTACAGAGA AAACACTTAC ATGTACAGGC CGGAGGAGCT CGATGTCGAC 
GCGAAGTTAA GTCGGCTCTG TGAGCAGGAC AAAGTAGTGC GAACTCAAGA AGAGAAACTG 
CAGCAGCTCC ACAGAGAAAA GCACACACTG GAGACGGCGC TGCTCTCCGC CAGTCAGGAG 
ATCGAGATGA GCTCTGAAAA CCCAGCGGCG GTCCAGAGCG TGGTCCAGCA GAGGGACGTC 
CTGCAGAGTG GCCTGCTGAG CACCTGCAGA GAGCTGAGCC GCGTCAGCAC TGAGCTGGAG 
CGAAGCTGGA GAGAATATGA TCGACTAGAG GCCGATGTTA CACTTGCCAA AACTAACCTG 
CTGGAGCAGC TGGAGGCCCT CGGCAGCCCA CAGACGGAGC CGCCCAGTCA AAAGCATGTT 
CAGATCCAGA AAGAGTTGTG GAGAATTCAG GATGTGATGG AGGCGCTGAG CAAGAACAAA 
CCCAAGAGAA ACCCTGAACC CAGTTTTCCT GGTGCAAACC CTCTGTCAAC CCTGCATAAA 
AGTGAAGACT CTGTAGACTC CTCGTGTGTT CCTCCTCGTC CTCCTCTGCC GTACTCATAT 
GAAGGAGGCG ATCGACCACC GCCCCTCCCC AACCCCGGTC AACCGCACAC CACCCGCACA 
ACACCACACC GACCAGAGGA CCGCAAAACT GCCCAACGCA ACGGCACGCA CACCGGCCCG 
GATTACAGGC TGTATAAGAG TGAGCCGGAG CTGACCACAG TAGCCGAAGT CGACGAGAGT 
AACGGTGAAG ACAAATCAGA GCAGACAGCA GAGAAAGAGC CGCCCGGCGT CACTAAAGGT 
ATAGTTTACC CGGTGGGAGT GGTCAGTCCC AGAACTAAAT CACCCATGCC CGAGTCCTCC 
ACCATCGCCT CCTACGTCAC CTTGAGGAAG ACCAAAAAAC CTGATCCCAG AACATGTCAG 
CCGCAGGACC GTCCGCGCAG CGCTGTGGAG CAGATGAGCA GTGGTGCTGA AGTGGGCCGA 
ACACGCATGA GTGTGGAGGA GCAGATGGAG AGAATCAGAC GACACCAGCA GGGGGCGCTG 
AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA TCCCGCAGCC TGTCCTTCAC TAAGGACAGC 
ACATACTACA CACTGCAGGC ACGTAAGAAG AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC 
GAGCGCAGAG ACATGACGCC TGTGGAGCGA GACACAAAAC ACACAAAACA CCCTGACGCA 
GAGGAAAACT GCAGAGACAC TAAGGAGCAG CTGCCAGCAC ACACCCACCT GGAGATTAAG 
GACAAAGCAT CTGTGACTCG CTCGGTCTCC ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT 
CCCAAACCCG TGCTGAAGCA GGACGAATCT GAGTGTAAAC TGGAGTACGA CAGAAAGCTG 
GCCACTCAGA ACAGCCTGCA GACGGCAGTG ATGGTGCGTG TGGGCACTGA GGAGGATGAA 
GAGGATGAAG AAGAGGAGCA GAAAGACTCA GTTCAGCAGC AGGAGGACTC TTATGAGCTC 
AGCAACAGCA AACACAGCAC ACTGATCTCA GCATCATGTG ACAGGAAGAA CACATCATCA 
CCATCGTCAT CATCAGACTG GAAGTCTCGC CCCCTCCTTC ATCAGCTGCC CAACACAAAC 
ACAACAGCGT CCAGGCCTCA GTTCAACAGC GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475086.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330761.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X11, mRNA.

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CloneID	ODa142029
Clone ID Related Accession (Same CDS sequence)	XM_021475086.1
Accession Version	XM_021475086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT 
CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG 
AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG 
CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC 
GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG 
CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA 
AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG 
GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG 
ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC 
AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT 
GAAGACTCTG TAGACTCCTC GTGTGTTCCT CCTCGTCCTC CTCTGCCGTA CTCATATGAA 
GGAGGCGATC GACCACCGCC CCTCCCCAAC CCCGGTCAAC CGCACACCAC CCGCACAACA 
CCACACCGAC CAGAGGACCG CAAAACTGCC CAACGCAACG GCACGCACAC CGGCCCGGAT 
TACAGGCTGT ATAAGAGTGA GCCGGAGCTG ACCACAGTAG CCGAAGTCGA CGAGAGTAAC 
GGTGAAGACA AATCAGAGCA GACAGCAGAG AAAGAGCCGC CCGGCGTCAC TAAAGGTATA 
GTTTACCCGG TGGGAGTGGT CAGTCCCAGA ACTAAATCAC CCATGCCCGA GTCCTCCACC 
ATCGCCTCCT ACGTCACCTT GAGGAAGACC AAAAAACCTG ATCCCAGAAC ATGTCAGCCG 
CAGGACCGTC CGCGCAGCGC TGTGGAGCAG ATGAGCAGTG GTGCTGAAGT GGGCCGAACA 
CGCATGAGTG TGGAGGAGCA GATGGAGAGA ATCAGACGAC ACCAGCAGGG GGCGCTGAGA 
GAGCGCAGGA GAGAAGATGG CTCTCTATCC CGCAGCCTGT CCTTCACTAA GGACAGCACA 
TACTACACAC TGCAGGCACG TAAGAAGAGT CCGGTGATCT CTCCAGACGA GCAGGACGAG 
CGCAGAGACA TGACGCCTGT GGAGCGAGAC ACAAAACACA CAAAACACCC TGACGCAGAG 
GAAAACTGCA GAGACACTAA GGAGCAGCTG CCAGCACACA CCCACCTGGA GATTAAGGAC 
AAAGCATCTG TGACTCGCTC GGTCTCCATG AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC 
AAACCCGTGC TGAAGCAGGA CGAATCTGAG TGTAAACTGG AGTACGACAG AAAGCTGGCC 
ACTCAGAACA GCCTGCAGAC GGCAGTGATG GTGCGTGTGG GCACTGAGGA GGATGAAGAG 
GATGAAGAAG AGGAGCAGAA AGACTCAGTT CAGCAGCAGG AGGACTCTTA TGAGCTCAGC 
AACAGCAAAC ACAGCACACT GATCTCAGCA TCATGTGACA GGAAGAACAC ATCATCACCA 
TCGTCATCAT CAGACTGGAA GTCTCGCCCC CTCCTTCATC AGCTGCCCAA CACAAACACA 
ACAGCGTCCA GGCCTCAGTT CAACAGCGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330761.1
CDS	42..4034
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTSEILSHHLQRNLMYLDSQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475086.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475086.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT 
CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG 
AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG 
CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC 
GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG 
CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA 
AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG 
GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG 
ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC 
AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT 
GAAGACTCTG TAGACTCCTC GTGTGTTCCT CCTCGTCCTC CTCTGCCGTA CTCATATGAA 
GGAGGCGATC GACCACCGCC CCTCCCCAAC CCCGGTCAAC CGCACACCAC CCGCACAACA 
CCACACCGAC CAGAGGACCG CAAAACTGCC CAACGCAACG GCACGCACAC CGGCCCGGAT 
TACAGGCTGT ATAAGAGTGA GCCGGAGCTG ACCACAGTAG CCGAAGTCGA CGAGAGTAAC 
GGTGAAGACA AATCAGAGCA GACAGCAGAG AAAGAGCCGC CCGGCGTCAC TAAAGGTATA 
GTTTACCCGG TGGGAGTGGT CAGTCCCAGA ACTAAATCAC CCATGCCCGA GTCCTCCACC 
ATCGCCTCCT ACGTCACCTT GAGGAAGACC AAAAAACCTG ATCCCAGAAC ATGTCAGCCG 
CAGGACCGTC CGCGCAGCGC TGTGGAGCAG ATGAGCAGTG GTGCTGAAGT GGGCCGAACA 
CGCATGAGTG TGGAGGAGCA GATGGAGAGA ATCAGACGAC ACCAGCAGGG GGCGCTGAGA 
GAGCGCAGGA GAGAAGATGG CTCTCTATCC CGCAGCCTGT CCTTCACTAA GGACAGCACA 
TACTACACAC TGCAGGCACG TAAGAAGAGT CCGGTGATCT CTCCAGACGA GCAGGACGAG 
CGCAGAGACA TGACGCCTGT GGAGCGAGAC ACAAAACACA CAAAACACCC TGACGCAGAG 
GAAAACTGCA GAGACACTAA GGAGCAGCTG CCAGCACACA CCCACCTGGA GATTAAGGAC 
AAAGCATCTG TGACTCGCTC GGTCTCCATG AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC 
AAACCCGTGC TGAAGCAGGA CGAATCTGAG TGTAAACTGG AGTACGACAG AAAGCTGGCC 
ACTCAGAACA GCCTGCAGAC GGCAGTGATG GTGCGTGTGG GCACTGAGGA GGATGAAGAG 
GATGAAGAAG AGGAGCAGAA AGACTCAGTT CAGCAGCAGG AGGACTCTTA TGAGCTCAGC 
AACAGCAAAC ACAGCACACT GATCTCAGCA TCATGTGACA GGAAGAACAC ATCATCACCA 
TCGTCATCAT CAGACTGGAA GTCTCGCCCC CTCCTTCATC AGCTGCCCAA CACAAACACA 
ACAGCGTCCA GGCCTCAGTT CAACAGCGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475087.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330762.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa142030
Clone ID Related Accession (Same CDS sequence)	XM_021475087.1
Accession Version	XM_021475087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAGA TGTGCTGCCA CAATGAGCGA AAGGTGACCT GCAAGCATCC CGTCAGCGGC 
GTCCCGTCAC AAGACAACTG CATCTTTGTG GTCAACGAAC ACCTGAATTG TGGGAATCTT 
GTCCATCCTG TTTTAAGCAG ACCAGCGACC AAAGCACCAG AGGCGGAGAA GAAGGAGCGT 
CCCACCAGCA CCATGAGCGA AGCCTCAAAC TACACCGGCG GCTCCGACTA CACCACACAT 
CCCAGCAGCC CCACAACCAG ACCATCCCGG TCCTCAAAGA AAGTGCACAA CTTTGGGAAA 
AGGTCCAACT CCATCAAGAG GAACCCCAAT GCACCTGTGA TCAAAAACGG CTGGCTTCAC 
AAACAGGACA GCACTGGGAT GAAGATGTGG AAGAAGAGAT GGTTCGTTCT GTCTGATATG 
TGCCTCTTCT ACTACAGAGA TGAGAAGGAA GAGGGGATTC TGGGAAGCAT CCTCCTGCCT 
AGCTTTCACA TCTCCATGCT GTCCGTGGAC GACCATATTA CCCGGAAATA CGCCTTCAAG 
GCGACGCATC CAAACATGCG GACGTATTAT TTCAGCACAG ACACAGCCAA AGATATGGAG 
TCCTGGATGA AAGTGATGTC GGATGCGGCG ATGGCTCACA GCGAGCCAAT CAGAAGGCTT 
GAGAAGGTAA AGCTGGACTC TCGCGTTCCT CAGGAGATGA ACAACATGCT GAACCACCGC 
GTCTTGACAC GACCCGAGAT CCAAAACAAC GAGAGGAACC GCGAGACGCT CCGCCAAGAG 
GACAAGAAGA GCAAACCATC AGAGAAACCT CAGAAAGACA AAGACAGAGT CAGCATGCAG 
AAAGACGGAG AGCGCTTCAG TGAAAACTAC ACTCACCGGC AGAAAGACGG CGAGCGCTAC 
ATGCTGCAGA AAGACCTGGA GAAATTTGTC CTGCAGAAAG ACGGAGAGAA GTACGCCGTG 
CAGAAAGAAG GCGACAAATA TCTCCTGCAG AAAGACGGAC ACAAATACAC AGTGCACAAA 
GACGGAGACA AATACACGCT TCAGAGAGAA CTCGAGATGT ACAATCCACC AAAAGACGCG 
GAGAAGATCC CGCAGAAAGA TGCAAGGTTT AGTCTGGTGC AGAAGGAGCT GGAGAAACCC 
GTCCCGCCAC CGCGATCTGA GGAAAAATAC GGCTTTCAGA AAGAGGGCAC CATTGAGCGT 
CCGCTAACTA AAATAAATAG CATTAAACTG CAGCCGGCTC AAGCAGCCGC CATCGCTGCA 
GCCGTTTCAT CCTCTAGGAT GATGCAGAAA CAGGTTAATG GCTCCGGGGA ACGAGCGGGA 
GACAGAAGTC CTGGAGATTT GAGTACTGTG AGGACGAGCG TCCCGCCACC AACGCAGCCG 
GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG 
GTTCGCACAC ACCGCACACG AGCTCCAGAC GACGACACCA GGAGCATTAC GTCCTACCAG 
ACCCTGCCAA GGAACATGCC GAGCCACCGG GCGCAGATTG TCCCTCGCTA CCCTGAGGGC 
TACCGCACTC TGCCCCGAAA CATGCTGCGT CCGGACAGCA TCTGCAGTGT AGCAGGATCA 
GTGTACGACC GAGCGCTTCA GCCCAGCACG CAGGAAAAGC GGCGCTCCAT CCGAGATGAC 
ACCATGTGGC AGCTGTACGA GTGGCAGCAG CGGCAGGCGC ACAGTCGGCT TGGGTACGGG 
ACGCTGCCCA GTCCCAAAAC TATGGGGCAG ATCGCGGAGT CCATCCCCAC GTCCCCATCC 
CATGGCTCTC TGGCCGGATA TCACACCTTC TCCCCGAACA GACCGCTCAA CCCTGACTCG 
CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT GGAGACGTGA CCATCGAGCG ACGCCACCGA 
CCACACCTGT CCAAGTACTC CTATCCTGCG GAACGCAGAG CTGCTCCTCC CGCACAGAAC 
ATCACTGCAC AGTCTCTTCA AGGCAAAACG CCAGAGGAAC TGACCCTGCT GCTCATCAAA 
CTGCGGCGGC AGCAGGCCGA GCTCAACAGC CTCCGTGAGC ACACACTCGC GCAGCTCATG 
CAGCTGAACC TCGACGCCAC CAACCCAAAG AGTGAAATCC TCTCTCATCA CCTGCAGAGG 
AATCTCATGT ACTTGGACAG TCAGATGAAG GAGAACGAAC CCATAATCTT CATGATTCAC 
ACTATGATTG AGAACTCGGC GCCCCGGCCG CAGCTCTACC AGCAACAGAT GAGTCCTGAA 
GAGTACAGAG AAAACACTTA CATGTACAGG CCGGAGGAGC TCGATGTCGA CGCGAAGTTA 
AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG CGAACTCAAG AAGAGAAACT GCAGCAGCTC 
CACAGAGAAA AGCACACACT GGAGACGGCG CTGCTCTCCG CCAGTCAGGA GATCGAGATG 
AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC GTGGTCCAGC AGAGGGACGT CCTGCAGAGT 
GGCCTGCTGA GCACCTGCAG AGAGCTGAGC CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG 
AGAGAATATG ATCGACTAGA GGCCGATGTT ACACTTGCCA AAACTAACCT GCTGGAGCAG 
CTGGAGGCCC TCGGCAGCCC ACAGACGGAG CCGCCCAGTC AAAAGCATGT TCAGATCCAG 
AAAGAGTTGT GGAGAATTCA GGATGTGATG GAGGCGCTGA GCAAGAACAA ACCCAAGAGA 
AACCCTGAAC CCAGTTTTCC TGGTGCAAAC CCTCTGTCAA CCCTGCATAA AAGTGAAGAC 
TCTGTAGACT CCTCGTGTGT TCCTCCTCGT CCTCCTCTGC CGTACTCATA TGAAGGAGGC 
GATCGACCAC CGCCCCTCCC CAACCCCGGT CAACCGCACA CCACCCGCAC AACACCACAC 
CGACCAGAGG ACCGCAAAAC TGCCCAACGC AACGGCACGC ACACCGGCCC GGATTACAGG 
CTGTATAAGA GTGAGCCGGA GCTGACCACA GTAGCCGAAG TCGACGAGAG TAACGGTGAA 
GACAAATCAG AGCAGACAGC AGAGAAAGAG CCGCCCGGCG TCACTAAAGG TATAGTTTAC 
CCGGTGGGAG TGGTCAGTCC CAGAACTAAA TCACCCATGC CCGAGTCCTC CACCATCGCC 
TCCTACGTCA CCTTGAGGAA GACCAAAAAA CCTGATCCCA GAACATGTCA GCCGCAGGAC 
CGTCCGCGCA GCGCTGTGGA GCAGATGAGC AGTGGTGCTG AAGTGGGCCG AACACGCATG 
AGTGTGGAGG AGCAGATGGA GAGAATCAGA CGACACCAGC AGGGGGCGCT GAGAGAGCGC 
AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC CTGTCCTTCA CTAAGGACAG CACATACTAC 
ACACTGCAGG CACGTAAGAA GAGTCCGGTG ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA 
GACATGACGC CTGTGGAGCG AGACACAAAA CACACAAAAC ACCCTGACGC AGAGGAAAAC 
TGCAGAGACA CTAAGGAGCA GCTGCCAGCA CACACCCACC TGGAGATTAA GGACAAAGCA 
TCTGTGACTC GCTCGGTCTC CATGAAAGAG TCTCTCCTGA AGAGTGTGAG TCCCAAACCC 
GTGCTGAAGC AGGACGAATC TGAGTGTAAA CTGGAGTACG ACAGAAAGCT GGCCACTCAG 
AACAGCCTGC AGACGGCAGT GATGGTGCGT GTGGGCACTG AGGAGGATGA AGAGGATGAA 
GAAGAGGAGC AGAAAGACTC AGTTCAGCAG CAGGAGGACT CTTATGAGCT CAGCAACAGC 
AAACACAGCA CACTGATCTC AGCATCATGT GACAGGAAGA ACACATCATC ACCATCGTCA 
TCATCAGACT GGAAGTCTCG CCCCCTCCTT CATCAGCTGC CCAACACAAA CACAACAGCG 
TCCAGGCCTC AGTTCAACAG CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330762.1
CDS	117..4103
Translation	MKQMCCHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475087.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475087.1

ORF Insert Sequence:

ATGAAGCAGA TGTGCTGCCA CAATGAGCGA AAGGTGACCT GCAAGCATCC CGTCAGCGGC 
GTCCCGTCAC AAGACAACTG CATCTTTGTG GTCAACGAAC ACCTGAATTG TGGGAATCTT 
GTCCATCCTG TTTTAAGCAG ACCAGCGACC AAAGCACCAG AGGCGGAGAA GAAGGAGCGT 
CCCACCAGCA CCATGAGCGA AGCCTCAAAC TACACCGGCG GCTCCGACTA CACCACACAT 
CCCAGCAGCC CCACAACCAG ACCATCCCGG TCCTCAAAGA AAGTGCACAA CTTTGGGAAA 
AGGTCCAACT CCATCAAGAG GAACCCCAAT GCACCTGTGA TCAAAAACGG CTGGCTTCAC 
AAACAGGACA GCACTGGGAT GAAGATGTGG AAGAAGAGAT GGTTCGTTCT GTCTGATATG 
TGCCTCTTCT ACTACAGAGA TGAGAAGGAA GAGGGGATTC TGGGAAGCAT CCTCCTGCCT 
AGCTTTCACA TCTCCATGCT GTCCGTGGAC GACCATATTA CCCGGAAATA CGCCTTCAAG 
GCGACGCATC CAAACATGCG GACGTATTAT TTCAGCACAG ACACAGCCAA AGATATGGAG 
TCCTGGATGA AAGTGATGTC GGATGCGGCG ATGGCTCACA GCGAGCCAAT CAGAAGGCTT 
GAGAAGGTAA AGCTGGACTC TCGCGTTCCT CAGGAGATGA ACAACATGCT GAACCACCGC 
GTCTTGACAC GACCCGAGAT CCAAAACAAC GAGAGGAACC GCGAGACGCT CCGCCAAGAG 
GACAAGAAGA GCAAACCATC AGAGAAACCT CAGAAAGACA AAGACAGAGT CAGCATGCAG 
AAAGACGGAG AGCGCTTCAG TGAAAACTAC ACTCACCGGC AGAAAGACGG CGAGCGCTAC 
ATGCTGCAGA AAGACCTGGA GAAATTTGTC CTGCAGAAAG ACGGAGAGAA GTACGCCGTG 
CAGAAAGAAG GCGACAAATA TCTCCTGCAG AAAGACGGAC ACAAATACAC AGTGCACAAA 
GACGGAGACA AATACACGCT TCAGAGAGAA CTCGAGATGT ACAATCCACC AAAAGACGCG 
GAGAAGATCC CGCAGAAAGA TGCAAGGTTT AGTCTGGTGC AGAAGGAGCT GGAGAAACCC 
GTCCCGCCAC CGCGATCTGA GGAAAAATAC GGCTTTCAGA AAGAGGGCAC CATTGAGCGT 
CCGCTAACTA AAATAAATAG CATTAAACTG CAGCCGGCTC AAGCAGCCGC CATCGCTGCA 
GCCGTTTCAT CCTCTAGGAT GATGCAGAAA CAGGTTAATG GCTCCGGGGA ACGAGCGGGA 
GACAGAAGTC CTGGAGATTT GAGTACTGTG AGGACGAGCG TCCCGCCACC AACGCAGCCG 
GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG 
GTTCGCACAC ACCGCACACG AGCTCCAGAC GACGACACCA GGAGCATTAC GTCCTACCAG 
ACCCTGCCAA GGAACATGCC GAGCCACCGG GCGCAGATTG TCCCTCGCTA CCCTGAGGGC 
TACCGCACTC TGCCCCGAAA CATGCTGCGT CCGGACAGCA TCTGCAGTGT AGCAGGATCA 
GTGTACGACC GAGCGCTTCA GCCCAGCACG CAGGAAAAGC GGCGCTCCAT CCGAGATGAC 
ACCATGTGGC AGCTGTACGA GTGGCAGCAG CGGCAGGCGC ACAGTCGGCT TGGGTACGGG 
ACGCTGCCCA GTCCCAAAAC TATGGGGCAG ATCGCGGAGT CCATCCCCAC GTCCCCATCC 
CATGGCTCTC TGGCCGGATA TCACACCTTC TCCCCGAACA GACCGCTCAA CCCTGACTCG 
CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT GGAGACGTGA CCATCGAGCG ACGCCACCGA 
CCACACCTGT CCAAGTACTC CTATCCTGCG GAACGCAGAG CTGCTCCTCC CGCACAGAAC 
ATCACTGCAC AGTCTCTTCA AGGCAAAACG CCAGAGGAAC TGACCCTGCT GCTCATCAAA 
CTGCGGCGGC AGCAGGCCGA GCTCAACAGC CTCCGTGAGC ACACACTCGC GCAGCTCATG 
CAGCTGAACC TCGACGCCAC CAACCCAAAG AGTGAAATCC TCTCTCATCA CCTGCAGAGG 
AATCTCATGT ACTTGGACAG TCAGATGAAG GAGAACGAAC CCATAATCTT CATGATTCAC 
ACTATGATTG AGAACTCGGC GCCCCGGCCG CAGCTCTACC AGCAACAGAT GAGTCCTGAA 
GAGTACAGAG AAAACACTTA CATGTACAGG CCGGAGGAGC TCGATGTCGA CGCGAAGTTA 
AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG CGAACTCAAG AAGAGAAACT GCAGCAGCTC 
CACAGAGAAA AGCACACACT GGAGACGGCG CTGCTCTCCG CCAGTCAGGA GATCGAGATG 
AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC GTGGTCCAGC AGAGGGACGT CCTGCAGAGT 
GGCCTGCTGA GCACCTGCAG AGAGCTGAGC CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG 
AGAGAATATG ATCGACTAGA GGCCGATGTT ACACTTGCCA AAACTAACCT GCTGGAGCAG 
CTGGAGGCCC TCGGCAGCCC ACAGACGGAG CCGCCCAGTC AAAAGCATGT TCAGATCCAG 
AAAGAGTTGT GGAGAATTCA GGATGTGATG GAGGCGCTGA GCAAGAACAA ACCCAAGAGA 
AACCCTGAAC CCAGTTTTCC TGGTGCAAAC CCTCTGTCAA CCCTGCATAA AAGTGAAGAC 
TCTGTAGACT CCTCGTGTGT TCCTCCTCGT CCTCCTCTGC CGTACTCATA TGAAGGAGGC 
GATCGACCAC CGCCCCTCCC CAACCCCGGT CAACCGCACA CCACCCGCAC AACACCACAC 
CGACCAGAGG ACCGCAAAAC TGCCCAACGC AACGGCACGC ACACCGGCCC GGATTACAGG 
CTGTATAAGA GTGAGCCGGA GCTGACCACA GTAGCCGAAG TCGACGAGAG TAACGGTGAA 
GACAAATCAG AGCAGACAGC AGAGAAAGAG CCGCCCGGCG TCACTAAAGG TATAGTTTAC 
CCGGTGGGAG TGGTCAGTCC CAGAACTAAA TCACCCATGC CCGAGTCCTC CACCATCGCC 
TCCTACGTCA CCTTGAGGAA GACCAAAAAA CCTGATCCCA GAACATGTCA GCCGCAGGAC 
CGTCCGCGCA GCGCTGTGGA GCAGATGAGC AGTGGTGCTG AAGTGGGCCG AACACGCATG 
AGTGTGGAGG AGCAGATGGA GAGAATCAGA CGACACCAGC AGGGGGCGCT GAGAGAGCGC 
AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC CTGTCCTTCA CTAAGGACAG CACATACTAC 
ACACTGCAGG CACGTAAGAA GAGTCCGGTG ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA 
GACATGACGC CTGTGGAGCG AGACACAAAA CACACAAAAC ACCCTGACGC AGAGGAAAAC 
TGCAGAGACA CTAAGGAGCA GCTGCCAGCA CACACCCACC TGGAGATTAA GGACAAAGCA 
TCTGTGACTC GCTCGGTCTC CATGAAAGAG TCTCTCCTGA AGAGTGTGAG TCCCAAACCC 
GTGCTGAAGC AGGACGAATC TGAGTGTAAA CTGGAGTACG ACAGAAAGCT GGCCACTCAG 
AACAGCCTGC AGACGGCAGT GATGGTGCGT GTGGGCACTG AGGAGGATGA AGAGGATGAA 
GAAGAGGAGC AGAAAGACTC AGTTCAGCAG CAGGAGGACT CTTATGAGCT CAGCAACAGC 
AAACACAGCA CACTGATCTC AGCATCATGT GACAGGAAGA ACACATCATC ACCATCGTCA 
TCATCAGACT GGAAGTCTCG CCCCCTCCTT CATCAGCTGC CCAACACAAA CACAACAGCG 
TCCAGGCCTC AGTTCAACAG CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475088.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330763.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa142031
Clone ID Related Accession (Same CDS sequence)	XM_021475088.1
Accession Version	XM_021475088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT 
CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG 
AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG 
CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC 
GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG 
CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA 
AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG 
GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG 
ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC 
AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT 
GAAGGCCCGG ATTACAGGCT GTATAAGAGT GAGCCGGAGC TGACCACAGT AGCCGAAGTC 
GACGAGAGTA ACGGTGAAGA CAAATCAGAG CAGACAGCAG AGAAAGAGCC GCCCGGCGTC 
ACTAAAGGTA TAGTTTACCC GGTGGGAGTG GTCAGTCCCA GAACTAAATC ACCCATGCCC 
GAGTCCTCCA CCATCGCCTC CTACGTCACC TTGAGGAAGA CCAAAAAACC TGATCCCAGA 
ACATGTCAGC CGCAGGACCG TCCGCGCAGC GCTGTGGAGC AGATGAGCAG TGGTGCTGAA 
GTGGGCCGAA CACGCATGAG TGTGGAGGAG CAGATGGAGA GAATCAGACG ACACCAGCAG 
GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT GGCTCTCTAT CCCGCAGCCT GTCCTTCACT 
AAGGACAGCA CATACTACAC ACTGCAGGCA CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC 
GAGCAGGACG AGCGCAGAGA CATGACGCCT GTGGAGCGAG ACACAAAACA CACAAAACAC 
CCTGACGCAG AGGAAAACTG CAGAGACACT AAGGAGCAGC TGCCAGCACA CACCCACCTG 
GAGATTAAGG ACAAAGCATC TGTGACTCGC TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG 
AGTGTGAGTC CCAAACCCGT GCTGAAGCAG GACGAATCTG AGTGTAAACT GGAGTACGAC 
AGAAAGCTGG CCACTCAGAA CAGCCTGCAG ACGGCAGTGA TGGTGCGTGT GGGCACTGAG 
GAGGATGAAG AGGATGAAGA AGAGGAGCAG AAAGACTCAG TTCAGCAGCA GGAGGACTCT 
TATGAGCTCA GCAACAGCAA ACACAGCACA CTGATCTCAG CATCATGTGA CAGGAAGAAC 
ACATCATCAC CATCGTCATC ATCAGACTGG AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC 
AACACAAACA CAACAGCGTC CAGGCCTCAG TTCAACAGCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330763.1
CDS	42..3986
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475088.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475088.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT 
CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG 
AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG 
CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC 
GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG 
CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA 
AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG 
GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG 
ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC 
AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT 
GAAGGCCCGG ATTACAGGCT GTATAAGAGT GAGCCGGAGC TGACCACAGT AGCCGAAGTC 
GACGAGAGTA ACGGTGAAGA CAAATCAGAG CAGACAGCAG AGAAAGAGCC GCCCGGCGTC 
ACTAAAGGTA TAGTTTACCC GGTGGGAGTG GTCAGTCCCA GAACTAAATC ACCCATGCCC 
GAGTCCTCCA CCATCGCCTC CTACGTCACC TTGAGGAAGA CCAAAAAACC TGATCCCAGA 
ACATGTCAGC CGCAGGACCG TCCGCGCAGC GCTGTGGAGC AGATGAGCAG TGGTGCTGAA 
GTGGGCCGAA CACGCATGAG TGTGGAGGAG CAGATGGAGA GAATCAGACG ACACCAGCAG 
GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT GGCTCTCTAT CCCGCAGCCT GTCCTTCACT 
AAGGACAGCA CATACTACAC ACTGCAGGCA CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC 
GAGCAGGACG AGCGCAGAGA CATGACGCCT GTGGAGCGAG ACACAAAACA CACAAAACAC 
CCTGACGCAG AGGAAAACTG CAGAGACACT AAGGAGCAGC TGCCAGCACA CACCCACCTG 
GAGATTAAGG ACAAAGCATC TGTGACTCGC TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG 
AGTGTGAGTC CCAAACCCGT GCTGAAGCAG GACGAATCTG AGTGTAAACT GGAGTACGAC 
AGAAAGCTGG CCACTCAGAA CAGCCTGCAG ACGGCAGTGA TGGTGCGTGT GGGCACTGAG 
GAGGATGAAG AGGATGAAGA AGAGGAGCAG AAAGACTCAG TTCAGCAGCA GGAGGACTCT 
TATGAGCTCA GCAACAGCAA ACACAGCACA CTGATCTCAG CATCATGTGA CAGGAAGAAC 
ACATCATCAC CATCGTCATC ATCAGACTGG AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC 
AACACAAACA CAACAGCGTC CAGGCCTCAG TTCAACAGCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475089.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330764.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X15, mRNA.

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CloneID	ODa142032
Clone ID Related Accession (Same CDS sequence)	XM_021475089.1
Accession Version	XM_021475089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CAGATGAGTC CTGAAGAGTA CAGAGAAAAC ACTTACATGT ACAGGCCGGA GGAGCTCGAT 
GTCGACGCGA AGTTAAGTCG GCTCTGTGAG CAGGACAAAG TAGTGCGAAC TCAAGAAGAG 
AAACTGCAGC AGCTCCACAG AGAAAAGCAC ACACTGGAGA CGGCGCTGCT CTCCGCCAGT 
CAGGAGATCG AGATGAGCTC TGAAAACCCA GCGGCGGTCC AGAGCGTGGT CCAGCAGAGG 
GACGTCCTGC AGAGTGGCCT GCTGAGCACC TGCAGAGAGC TGAGCCGCGT CAGCACTGAG 
CTGGAGCGAA GCTGGAGAGA ATATGATCGA CTAGAGGCCG ATGTTACACT TGCCAAAACT 
AACCTGCTGG AGCAGCTGGA GGCCCTCGGC AGCCCACAGA CGGAGCCGCC CAGTCAAAAG 
CATGTTCAGA TCCAGAAAGA GTTGTGGAGA ATTCAGGATG TGATGGAGGC GCTGAGCAAG 
AACAAACCCA AGAGAAACCC TGAACCCAGT TTTCCTGGTG CAAACCCTCT GTCAACCCTG 
CATAAAAGTG AAGACTCTGT AGACTCCTCG TGTGTTCCTC CTCGTCCTCC TCTGCCGTAC 
TCATATGAAG GAGGCGATCG ACCACCGCCC CTCCCCAACC CCGGTCAACC GCACACCACC 
CGCACAACAC CACACCGACC AGAGGACCGC AAAACTGCCC AACGCAACGG CACGCACACC 
GGCCCGGATT ACAGGCTGTA TAAGAGTGAG CCGGAGCTGA CCACAGTAGC CGAAGTCGAC 
GAGAGTAACG GTGAAGACAA ATCAGAGCAG ACAGCAGAGA AAGAGCCGCC CGGCGTCACT 
AAAGGTATAG TTTACCCGGT GGGAGTGGTC AGTCCCAGAA CTAAATCACC CATGCCCGAG 
TCCTCCACCA TCGCCTCCTA CGTCACCTTG AGGAAGACCA AAAAACCTGA TCCCAGAACA 
TGTCAGCCGC AGGACCGTCC GCGCAGCGCT GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG 
GGCCGAACAC GCATGAGTGT GGAGGAGCAG ATGGAGAGAA TCAGACGACA CCAGCAGGGG 
GCGCTGAGAG AGCGCAGGAG AGAAGATGGC TCTCTATCCC GCAGCCTGTC CTTCACTAAG 
GACAGCACAT ACTACACACT GCAGGCACGT AAGAAGAGTC CGGTGATCTC TCCAGACGAG 
CAGGACGAGC GCAGAGACAT GACGCCTGTG GAGCGAGACA CAAAACACAC AAAACACCCT 
GACGCAGAGG AAAACTGCAG AGACACTAAG GAGCAGCTGC CAGCACACAC CCACCTGGAG 
ATTAAGGACA AAGCATCTGT GACTCGCTCG GTCTCCATGA AAGAGTCTCT CCTGAAGAGT 
GTGAGTCCCA AACCCGTGCT GAAGCAGGAC GAATCTGAGT GTAAACTGGA GTACGACAGA 
AAGCTGGCCA CTCAGAACAG CCTGCAGACG GCAGTGATGG TGCGTGTGGG CACTGAGGAG 
GATGAAGAGG ATGAAGAAGA GGAGCAGAAA GACTCAGTTC AGCAGCAGGA GGACTCTTAT 
GAGCTCAGCA ACAGCAAACA CAGCACACTG ATCTCAGCAT CATGTGACAG GAAGAACACA 
TCATCACCAT CGTCATCATC AGACTGGAAG TCTCGCCCCC TCCTTCATCA GCTGCCCAAC 
ACAAACACAA CAGCGTCCAG GCCTCAGTTC AACAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330764.1
CDS	42..3983
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475089.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475089.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CAGATGAGTC CTGAAGAGTA CAGAGAAAAC ACTTACATGT ACAGGCCGGA GGAGCTCGAT 
GTCGACGCGA AGTTAAGTCG GCTCTGTGAG CAGGACAAAG TAGTGCGAAC TCAAGAAGAG 
AAACTGCAGC AGCTCCACAG AGAAAAGCAC ACACTGGAGA CGGCGCTGCT CTCCGCCAGT 
CAGGAGATCG AGATGAGCTC TGAAAACCCA GCGGCGGTCC AGAGCGTGGT CCAGCAGAGG 
GACGTCCTGC AGAGTGGCCT GCTGAGCACC TGCAGAGAGC TGAGCCGCGT CAGCACTGAG 
CTGGAGCGAA GCTGGAGAGA ATATGATCGA CTAGAGGCCG ATGTTACACT TGCCAAAACT 
AACCTGCTGG AGCAGCTGGA GGCCCTCGGC AGCCCACAGA CGGAGCCGCC CAGTCAAAAG 
CATGTTCAGA TCCAGAAAGA GTTGTGGAGA ATTCAGGATG TGATGGAGGC GCTGAGCAAG 
AACAAACCCA AGAGAAACCC TGAACCCAGT TTTCCTGGTG CAAACCCTCT GTCAACCCTG 
CATAAAAGTG AAGACTCTGT AGACTCCTCG TGTGTTCCTC CTCGTCCTCC TCTGCCGTAC 
TCATATGAAG GAGGCGATCG ACCACCGCCC CTCCCCAACC CCGGTCAACC GCACACCACC 
CGCACAACAC CACACCGACC AGAGGACCGC AAAACTGCCC AACGCAACGG CACGCACACC 
GGCCCGGATT ACAGGCTGTA TAAGAGTGAG CCGGAGCTGA CCACAGTAGC CGAAGTCGAC 
GAGAGTAACG GTGAAGACAA ATCAGAGCAG ACAGCAGAGA AAGAGCCGCC CGGCGTCACT 
AAAGGTATAG TTTACCCGGT GGGAGTGGTC AGTCCCAGAA CTAAATCACC CATGCCCGAG 
TCCTCCACCA TCGCCTCCTA CGTCACCTTG AGGAAGACCA AAAAACCTGA TCCCAGAACA 
TGTCAGCCGC AGGACCGTCC GCGCAGCGCT GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG 
GGCCGAACAC GCATGAGTGT GGAGGAGCAG ATGGAGAGAA TCAGACGACA CCAGCAGGGG 
GCGCTGAGAG AGCGCAGGAG AGAAGATGGC TCTCTATCCC GCAGCCTGTC CTTCACTAAG 
GACAGCACAT ACTACACACT GCAGGCACGT AAGAAGAGTC CGGTGATCTC TCCAGACGAG 
CAGGACGAGC GCAGAGACAT GACGCCTGTG GAGCGAGACA CAAAACACAC AAAACACCCT 
GACGCAGAGG AAAACTGCAG AGACACTAAG GAGCAGCTGC CAGCACACAC CCACCTGGAG 
ATTAAGGACA AAGCATCTGT GACTCGCTCG GTCTCCATGA AAGAGTCTCT CCTGAAGAGT 
GTGAGTCCCA AACCCGTGCT GAAGCAGGAC GAATCTGAGT GTAAACTGGA GTACGACAGA 
AAGCTGGCCA CTCAGAACAG CCTGCAGACG GCAGTGATGG TGCGTGTGGG CACTGAGGAG 
GATGAAGAGG ATGAAGAAGA GGAGCAGAAA GACTCAGTTC AGCAGCAGGA GGACTCTTAT 
GAGCTCAGCA ACAGCAAACA CAGCACACTG ATCTCAGCAT CATGTGACAG GAAGAACACA 
TCATCACCAT CGTCATCATC AGACTGGAAG TCTCGCCCCC TCCTTCATCA GCTGCCCAAC 
ACAAACACAA CAGCGTCCAG GCCTCAGTTC AACAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475090.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330765.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ODa142033
Clone ID Related Accession (Same CDS sequence)	XM_021475090.1
Accession Version	XM_021475090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
ATGAGTCCTG AAGAGTACAG AGAAAACACT TACATGTACA GGCCGGAGGA GCTCGATGTC 
GACGCGAAGT TAAGTCGGCT CTGTGAGCAG GACAAAGTAG TGCGAACTCA AGAAGAGAAA 
CTGCAGCAGC TCCACAGAGA AAAGCACACA CTGGAGACGG CGCTGCTCTC CGCCAGTCAG 
GAGATCGAGA TGAGCTCTGA AAACCCAGCG GCGGTCCAGA GCGTGGTCCA GCAGAGGGAC 
GTCCTGCAGA GTGGCCTGCT GAGCACCTGC AGAGAGCTGA GCCGCGTCAG CACTGAGCTG 
GAGCGAAGCT GGAGAGAATA TGATCGACTA GAGGCCGATG TTACACTTGC CAAAACTAAC 
CTGCTGGAGC AGCTGGAGGC CCTCGGCAGC CCACAGACGG AGCCGCCCAG TCAAAAGCAT 
GTTCAGATCC AGAAAGAGTT GTGGAGAATT CAGGATGTGA TGGAGGCGCT GAGCAAGAAC 
AAACCCAAGA GAAACCCTGA ACCCAGTTTT CCTGGTGCAA ACCCTCTGTC AACCCTGCAT 
AAAAGTGAAG ACTCTGTAGA CTCCTCGTGT GTTCCTCCTC GTCCTCCTCT GCCGTACTCA 
TATGAAGGAG GCGATCGACC ACCGCCCCTC CCCAACCCCG GTCAACCGCA CACCACCCGC 
ACAACACCAC ACCGACCAGA GGACCGCAAA ACTGCCCAAC GCAACGGCAC GCACACCGGC 
CCGGATTACA GGCTGTATAA GAGTGAGCCG GAGCTGACCA CAGTAGCCGA AGTCGACGAG 
AGTAACGGTG AAGACAAATC AGAGCAGACA GCAGAGAAAG AGCCGCCCGG CGTCACTAAA 
GGTATAGTTT ACCCGGTGGG AGTGGTCAGT CCCAGAACTA AATCACCCAT GCCCGAGTCC 
TCCACCATCG CCTCCTACGT CACCTTGAGG AAGACCAAAA AACCTGATCC CAGAACATGT 
CAGCCGCAGG ACCGTCCGCG CAGCGCTGTG GAGCAGATGA GCAGTGGTGC TGAAGTGGGC 
CGAACACGCA TGAGTGTGGA GGAGCAGATG GAGAGAATCA GACGACACCA GCAGGGGGCG 
CTGAGAGAGC GCAGGAGAGA AGATGGCTCT CTATCCCGCA GCCTGTCCTT CACTAAGGAC 
AGCACATACT ACACACTGCA GGCACGTAAG AAGAGTCCGG TGATCTCTCC AGACGAGCAG 
GACGAGCGCA GAGACATGAC GCCTGTGGAG CGAGACACAA AACACACAAA ACACCCTGAC 
GCAGAGGAAA ACTGCAGAGA CACTAAGGAG CAGCTGCCAG CACACACCCA CCTGGAGATT 
AAGGACAAAG CATCTGTGAC TCGCTCGGTC TCCATGAAAG AGTCTCTCCT GAAGAGTGTG 
AGTCCCAAAC CCGTGCTGAA GCAGGACGAA TCTGAGTGTA AACTGGAGTA CGACAGAAAG 
CTGGCCACTC AGAACAGCCT GCAGACGGCA GTGATGGTGC GTGTGGGCAC TGAGGAGGAT 
GAAGAGGATG AAGAAGAGGA GCAGAAAGAC TCAGTTCAGC AGCAGGAGGA CTCTTATGAG 
CTCAGCAACA GCAAACACAG CACACTGATC TCAGCATCAT GTGACAGGAA GAACACATCA 
TCACCATCGT CATCATCAGA CTGGAAGTCT CGCCCCCTCC TTCATCAGCT GCCCAACACA 
AACACAACAG CGTCCAGGCC TCAGTTCAAC AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330765.1
CDS	42..3980
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475090.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475090.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
ATGAGTCCTG AAGAGTACAG AGAAAACACT TACATGTACA GGCCGGAGGA GCTCGATGTC 
GACGCGAAGT TAAGTCGGCT CTGTGAGCAG GACAAAGTAG TGCGAACTCA AGAAGAGAAA 
CTGCAGCAGC TCCACAGAGA AAAGCACACA CTGGAGACGG CGCTGCTCTC CGCCAGTCAG 
GAGATCGAGA TGAGCTCTGA AAACCCAGCG GCGGTCCAGA GCGTGGTCCA GCAGAGGGAC 
GTCCTGCAGA GTGGCCTGCT GAGCACCTGC AGAGAGCTGA GCCGCGTCAG CACTGAGCTG 
GAGCGAAGCT GGAGAGAATA TGATCGACTA GAGGCCGATG TTACACTTGC CAAAACTAAC 
CTGCTGGAGC AGCTGGAGGC CCTCGGCAGC CCACAGACGG AGCCGCCCAG TCAAAAGCAT 
GTTCAGATCC AGAAAGAGTT GTGGAGAATT CAGGATGTGA TGGAGGCGCT GAGCAAGAAC 
AAACCCAAGA GAAACCCTGA ACCCAGTTTT CCTGGTGCAA ACCCTCTGTC AACCCTGCAT 
AAAAGTGAAG ACTCTGTAGA CTCCTCGTGT GTTCCTCCTC GTCCTCCTCT GCCGTACTCA 
TATGAAGGAG GCGATCGACC ACCGCCCCTC CCCAACCCCG GTCAACCGCA CACCACCCGC 
ACAACACCAC ACCGACCAGA GGACCGCAAA ACTGCCCAAC GCAACGGCAC GCACACCGGC 
CCGGATTACA GGCTGTATAA GAGTGAGCCG GAGCTGACCA CAGTAGCCGA AGTCGACGAG 
AGTAACGGTG AAGACAAATC AGAGCAGACA GCAGAGAAAG AGCCGCCCGG CGTCACTAAA 
GGTATAGTTT ACCCGGTGGG AGTGGTCAGT CCCAGAACTA AATCACCCAT GCCCGAGTCC 
TCCACCATCG CCTCCTACGT CACCTTGAGG AAGACCAAAA AACCTGATCC CAGAACATGT 
CAGCCGCAGG ACCGTCCGCG CAGCGCTGTG GAGCAGATGA GCAGTGGTGC TGAAGTGGGC 
CGAACACGCA TGAGTGTGGA GGAGCAGATG GAGAGAATCA GACGACACCA GCAGGGGGCG 
CTGAGAGAGC GCAGGAGAGA AGATGGCTCT CTATCCCGCA GCCTGTCCTT CACTAAGGAC 
AGCACATACT ACACACTGCA GGCACGTAAG AAGAGTCCGG TGATCTCTCC AGACGAGCAG 
GACGAGCGCA GAGACATGAC GCCTGTGGAG CGAGACACAA AACACACAAA ACACCCTGAC 
GCAGAGGAAA ACTGCAGAGA CACTAAGGAG CAGCTGCCAG CACACACCCA CCTGGAGATT 
AAGGACAAAG CATCTGTGAC TCGCTCGGTC TCCATGAAAG AGTCTCTCCT GAAGAGTGTG 
AGTCCCAAAC CCGTGCTGAA GCAGGACGAA TCTGAGTGTA AACTGGAGTA CGACAGAAAG 
CTGGCCACTC AGAACAGCCT GCAGACGGCA GTGATGGTGC GTGTGGGCAC TGAGGAGGAT 
GAAGAGGATG AAGAAGAGGA GCAGAAAGAC TCAGTTCAGC AGCAGGAGGA CTCTTATGAG 
CTCAGCAACA GCAAACACAG CACACTGATC TCAGCATCAT GTGACAGGAA GAACACATCA 
TCACCATCGT CATCATCAGA CTGGAAGTCT CGCCCCCTCC TTCATCAGCT GCCCAACACA 
AACACAACAG CGTCCAGGCC TCAGTTCAAC AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475091.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330766.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X20, mRNA.

Price & Availability↑

CloneID	ODa142034
Clone ID Related Accession (Same CDS sequence)	XM_021475091.1
Accession Version	XM_021475091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
ATGAGTCCTG AAGAGTACAG AGAAAACACT TACATGTACA GGCCGGAGGA GCTCGATGTC 
GACGCGAAGT TAAGTCGGCT CTGTGAGCAG GACAAAGTAG TGCGAACTCA AGAAGAGAAA 
CTGCAGCAGC TCCACAGAGA AAAGCACACA CTGGAGACGG CGCTGCTCTC CGCCAGTCAG 
GAGATCGAGA TGAGCTCTGA AAACCCAGCG GCGGTCCAGA GCGTGGTCCA GCAGAGGGAC 
GTCCTGCAGA GTGGCCTGCT GAGCACCTGC AGAGAGCTGA GCCGCGTCAG CACTGAGCTG 
GAGCGAAGCT GGAGAGAATA TGATCGACTA GAGGCCGATG TTACACTTGC CAAAACTAAC 
CTGCTGGAGC AGCTGGAGGC CCTCGGCAGC CCACAGACGG AGCCGCCCAG TCAAAAGCAT 
GTTCAGATCC AGAAAGAGTT GTGGAGAATT CAGGATGTGA TGGAGGCGCT GAGCAAGAAC 
AAACCCAAGA GAAACCCTGA ACCCAGTTTT CCTGGTGCAA ACCCTCTGTC AACCCTGCAT 
AAAAGTGAAG GCCCGGATTA CAGGCTGTAT AAGAGTGAGC CGGAGCTGAC CACAGTAGCC 
GAAGTCGACG AGAGTAACGG TGAAGACAAA TCAGAGCAGA CAGCAGAGAA AGAGCCGCCC 
GGCGTCACTA AAGGTATAGT TTACCCGGTG GGAGTGGTCA GTCCCAGAAC TAAATCACCC 
ATGCCCGAGT CCTCCACCAT CGCCTCCTAC GTCACCTTGA GGAAGACCAA AAAACCTGAT 
CCCAGAACAT GTCAGCCGCA GGACCGTCCG CGCAGCGCTG TGGAGCAGAT GAGCAGTGGT 
GCTGAAGTGG GCCGAACACG CATGAGTGTG GAGGAGCAGA TGGAGAGAAT CAGACGACAC 
CAGCAGGGGG CGCTGAGAGA GCGCAGGAGA GAAGATGGCT CTCTATCCCG CAGCCTGTCC 
TTCACTAAGG ACAGCACATA CTACACACTG CAGGCACGTA AGAAGAGTCC GGTGATCTCT 
CCAGACGAGC AGGACGAGCG CAGAGACATG ACGCCTGTGG AGCGAGACAC AAAACACACA 
AAACACCCTG ACGCAGAGGA AAACTGCAGA GACACTAAGG AGCAGCTGCC AGCACACACC 
CACCTGGAGA TTAAGGACAA AGCATCTGTG ACTCGCTCGG TCTCCATGAA AGAGTCTCTC 
CTGAAGAGTG TGAGTCCCAA ACCCGTGCTG AAGCAGGACG AATCTGAGTG TAAACTGGAG 
TACGACAGAA AGCTGGCCAC TCAGAACAGC CTGCAGACGG CAGTGATGGT GCGTGTGGGC 
ACTGAGGAGG ATGAAGAGGA TGAAGAAGAG GAGCAGAAAG ACTCAGTTCA GCAGCAGGAG 
GACTCTTATG AGCTCAGCAA CAGCAAACAC AGCACACTGA TCTCAGCATC ATGTGACAGG 
AAGAACACAT CATCACCATC GTCATCATCA GACTGGAAGT CTCGCCCCCT CCTTCATCAG 
CTGCCCAACA CAAACACAAC AGCGTCCAGG CCTCAGTTCA ACAGCGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330766.1
CDS	42..3812
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_021475091.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475091.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
ATGAGTCCTG AAGAGTACAG AGAAAACACT TACATGTACA GGCCGGAGGA GCTCGATGTC 
GACGCGAAGT TAAGTCGGCT CTGTGAGCAG GACAAAGTAG TGCGAACTCA AGAAGAGAAA 
CTGCAGCAGC TCCACAGAGA AAAGCACACA CTGGAGACGG CGCTGCTCTC CGCCAGTCAG 
GAGATCGAGA TGAGCTCTGA AAACCCAGCG GCGGTCCAGA GCGTGGTCCA GCAGAGGGAC 
GTCCTGCAGA GTGGCCTGCT GAGCACCTGC AGAGAGCTGA GCCGCGTCAG CACTGAGCTG 
GAGCGAAGCT GGAGAGAATA TGATCGACTA GAGGCCGATG TTACACTTGC CAAAACTAAC 
CTGCTGGAGC AGCTGGAGGC CCTCGGCAGC CCACAGACGG AGCCGCCCAG TCAAAAGCAT 
GTTCAGATCC AGAAAGAGTT GTGGAGAATT CAGGATGTGA TGGAGGCGCT GAGCAAGAAC 
AAACCCAAGA GAAACCCTGA ACCCAGTTTT CCTGGTGCAA ACCCTCTGTC AACCCTGCAT 
AAAAGTGAAG GCCCGGATTA CAGGCTGTAT AAGAGTGAGC CGGAGCTGAC CACAGTAGCC 
GAAGTCGACG AGAGTAACGG TGAAGACAAA TCAGAGCAGA CAGCAGAGAA AGAGCCGCCC 
GGCGTCACTA AAGGTATAGT TTACCCGGTG GGAGTGGTCA GTCCCAGAAC TAAATCACCC 
ATGCCCGAGT CCTCCACCAT CGCCTCCTAC GTCACCTTGA GGAAGACCAA AAAACCTGAT 
CCCAGAACAT GTCAGCCGCA GGACCGTCCG CGCAGCGCTG TGGAGCAGAT GAGCAGTGGT 
GCTGAAGTGG GCCGAACACG CATGAGTGTG GAGGAGCAGA TGGAGAGAAT CAGACGACAC 
CAGCAGGGGG CGCTGAGAGA GCGCAGGAGA GAAGATGGCT CTCTATCCCG CAGCCTGTCC 
TTCACTAAGG ACAGCACATA CTACACACTG CAGGCACGTA AGAAGAGTCC GGTGATCTCT 
CCAGACGAGC AGGACGAGCG CAGAGACATG ACGCCTGTGG AGCGAGACAC AAAACACACA 
AAACACCCTG ACGCAGAGGA AAACTGCAGA GACACTAAGG AGCAGCTGCC AGCACACACC 
CACCTGGAGA TTAAGGACAA AGCATCTGTG ACTCGCTCGG TCTCCATGAA AGAGTCTCTC 
CTGAAGAGTG TGAGTCCCAA ACCCGTGCTG AAGCAGGACG AATCTGAGTG TAAACTGGAG 
TACGACAGAA AGCTGGCCAC TCAGAACAGC CTGCAGACGG CAGTGATGGT GCGTGTGGGC 
ACTGAGGAGG ATGAAGAGGA TGAAGAAGAG GAGCAGAAAG ACTCAGTTCA GCAGCAGGAG 
GACTCTTATG AGCTCAGCAA CAGCAAACAC AGCACACTGA TCTCAGCATC ATGTGACAGG 
AAGAACACAT CATCACCATC GTCATCATCA GACTGGAAGT CTCGCCCCCT CCTTCATCAG 
CTGCCCAACA CAAACACAAC AGCGTCCAGG CCTCAGTTCA ACAGCGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_021475093.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330768.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X24, mRNA.

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CloneID	ODa142035
Clone ID Related Accession (Same CDS sequence)	XM_021475093.1
Accession Version	XM_021475093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAAATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT 
CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA 
CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC 
GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA 
GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG 
CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA 
CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT 
GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT 
GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA 
CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG 
TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC 
TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC 
ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC 
ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT 
CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG 
GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG 
GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG 
GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGT GAACAGTGTG 
TCGACTCCTC CTCAATCTCC AAGCTCCTCC TCCTCTCCTC CGCCTCCACC CTCCCCTCCA 
CAGCTGACGG ACGGGTCACA CTTCATGTGT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330768.1
CDS	42..4217
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEHLNCGNLVHPVLSRPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISVNSVSTPPQSPSSSSSPPPPPSPPQLTDGSHFMCV

Target ORF information:

RefSeq Version	XM_021475093.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475093.1

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC 
AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC 
TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG 
TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT 
GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG 
AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA 
GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC 
GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT 
TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG 
ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT 
CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC 
GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT 
CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC 
ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC 
CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG 
AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA 
CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT 
AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC 
GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG 
CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA 
CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG 
AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA 
ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC 
GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG 
GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT 
CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG 
CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG 
CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG 
ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC 
TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT 
GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG 
GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG 
CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC 
CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG 
AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG 
GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG 
CAGCTCTACC AGCAAATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT 
CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA 
CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC 
GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA 
GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG 
CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA 
CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT 
GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT 
GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA 
CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG 
TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC 
TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC 
ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC 
ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT 
CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG 
GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG 
GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG 
GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGT GAACAGTGTG 
TCGACTCCTC CTCAATCTCC AAGCTCCTCC TCCTCTCCTC CGCCTCCACC CTCCCCTCCA 
CAGCTGACGG ACGGGTCACA CTTCATGTGT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009299998.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298273.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X2, mRNA.

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CloneID	ODa45107
Clone ID Related Accession (Same CDS sequence)	XM_009299998.2
Accession Version	XM_009299998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAAATGA GTCCTGAAGA GTACAGAGAA 
AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT 
GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG 
CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC 
CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC 
ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT 
CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC 
GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG 
AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC 
AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC 
TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG 
CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC 
CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT 
GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG 
CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG 
GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC 
TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC 
GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG 
CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT 
GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA 
CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT 
GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT 
AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC 
TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG 
GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG 
ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG 
AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA 
CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG 
AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG 
TTCAACAGCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298273.1
CDS	44..4198
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009299998.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299998.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAAATGA GTCCTGAAGA GTACAGAGAA 
AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT 
GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG 
CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC 
CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC 
ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT 
CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC 
GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG 
AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC 
AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC 
TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG 
CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC 
CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT 
GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG 
CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG 
GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC 
TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC 
GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG 
CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT 
GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA 
CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT 
GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT 
AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC 
TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG 
GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG 
ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG 
AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA 
CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG 
AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG 
TTCAACAGCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009299999.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298274.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa45108
Clone ID Related Accession (Same CDS sequence)	XM_009299999.2 , XM_009299999.3
Accession Version	XM_009299999.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA 
GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC 
TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA 
AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA 
AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG 
AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT 
GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC 
CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG 
TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA 
CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC 
TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA 
CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG 
GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG 
AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA 
GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA 
GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC 
ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC 
AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG 
GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA 
GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG 
GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG 
CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC 
ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT 
CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG 
CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG 
CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG 
CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC 
ACACTGATCT CAGTGAACAG TGTGTCGACT CCTCCTCAAT CTCCAAGCTC CTCCTCCTCT 
CCTCCGCCTC CACCCTCCCC TCCACAGCTG ACGGACGGGT CACACTTCAT GTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298274.1
CDS	44..4183
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISVNSVSTPPQSPSSSSSPPPPPSPPQLTDGSHFMCV

Target ORF information:

RefSeq Version	XM_009299999.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299999.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA 
GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC 
TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA 
AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA 
AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG 
AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT 
GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC 
CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG 
TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA 
CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC 
TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA 
CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG 
GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG 
AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA 
GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA 
GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC 
ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC 
AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG 
GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA 
GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG 
GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG 
CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC 
ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT 
CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG 
CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG 
CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG 
CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC 
ACACTGATCT CAGTGAACAG TGTGTCGACT CCTCCTCAAT CTCCAAGCTC CTCCTCCTCT 
CCTCCGCCTC CACCCTCCCC TCCACAGCTG ACGGACGGGT CACACTTCAT GTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009299999.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298274.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X22, mRNA.

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CloneID	ODa45108
Clone ID Related Accession (Same CDS sequence)	XM_009299999.2 , XM_009299999.3
Accession Version	XM_009299999.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA 
GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC 
TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA 
AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA 
AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG 
AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT 
GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC 
CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG 
TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA 
CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC 
TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA 
CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG 
GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG 
AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA 
GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA 
GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC 
ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC 
AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG 
GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA 
GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG 
GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG 
CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC 
ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT 
CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG 
CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG 
CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG 
CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC 
ACACTGATCT CAGTGAACAG TGTGTCGACT CCTCCTCAAT CTCCAAGCTC CTCCTCCTCT 
CCTCCGCCTC CACCCTCCCC TCCACAGCTG ACGGACGGGT CACACTTCAT GTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298274.1
CDS	42..4181
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISVNSVSTPPQSPSSSSSPPPPPSPPQLTDGSHFMCV

Target ORF information:

RefSeq Version	XM_009299999.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299999.3

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA 
GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC 
TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA 
AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA 
AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG 
AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT 
GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC 
CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG 
TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA 
CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC 
TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA 
CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG 
GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG 
AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA 
GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA 
GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC 
ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC 
AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG 
GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA 
GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG 
GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG 
CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC 
ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT 
CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG 
CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG 
CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG 
CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC 
ACACTGATCT CAGTGAACAG TGTGTCGACT CCTCCTCAAT CTCCAAGCTC CTCCTCCTCT 
CCTCCGCCTC CACCCTCCCC TCCACAGCTG ACGGACGGGT CACACTTCAT GTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300000.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298275.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X4, mRNA.

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CloneID	ODa45109
Clone ID Related Accession (Same CDS sequence)	XM_009300000.2
Accession Version	XM_009300000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGCAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG 
CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG 
CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG 
CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC 
GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC 
CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT 
ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG 
CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG 
GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC 
CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT 
CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT 
CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC 
AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA 
GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG 
CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA 
TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA 
CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC 
AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA 
CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC 
CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG 
ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA 
CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA 
CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG 
TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA 
CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT 
GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG 
CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT 
GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT 
CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298275.1
CDS	44..4120
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009300000.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300000.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGCAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG 
CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG 
CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG 
CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC 
GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC 
CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT 
ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG 
CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG 
GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC 
CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT 
CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT 
CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC 
AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA 
GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG 
CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA 
TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA 
CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC 
AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA 
CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC 
CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG 
ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA 
CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA 
CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG 
TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA 
CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT 
GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG 
CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT 
GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT 
CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300001.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298276.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X5, mRNA.

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CloneID	ODa45110
Clone ID Related Accession (Same CDS sequence)	XM_009300001.2
Accession Version	XM_009300001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT 
CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA 
CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC 
GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA 
GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG 
CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA 
CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT 
GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT 
GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA 
CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG 
TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC 
TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC 
ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC 
ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT 
CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG 
GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG 
GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG 
GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGC ATCATGTGAC 
AGGAAGAACA CATCATCACC ATCGTCATCA TCAGACTGGA AGTCTCGCCC CCTCCTTCAT 
CAGCTGCCCA ACACAAACAC AACAGCGTCC AGGCCTCAGT TCAACAGCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298276.1
CDS	44..4117
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009300001.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300001.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT 
CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA 
CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC 
GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA 
GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG 
CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA 
CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT 
GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT 
GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA 
CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG 
TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC 
TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC 
ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC 
ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT 
CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG 
GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG 
GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG 
GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGC ATCATGTGAC 
AGGAAGAACA CATCATCACC ATCGTCATCA TCAGACTGGA AGTCTCGCCC CCTCCTTCAT 
CAGCTGCCCA ACACAAACAC AACAGCGTCC AGGCCTCAGT TCAACAGCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300002.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298277.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa45111
Clone ID Related Accession (Same CDS sequence)	XM_009300002.2
Accession Version	XM_009300002.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA 
GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC 
TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA 
AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA 
AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG 
AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT 
GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC 
CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG 
TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA 
CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGG CCCGGATTAC 
AGGCTGTATA AGAGTGAGCC GGAGCTGACC ACAGTAGCCG AAGTCGACGA GAGTAACGGT 
GAAGACAAAT CAGAGCAGAC AGCAGAGAAA GAGCCGCCCG GCGTCACTAA AGGTATAGTT 
TACCCGGTGG GAGTGGTCAG TCCCAGAACT AAATCACCCA TGCCCGAGTC CTCCACCATC 
GCCTCCTACG TCACCTTGAG GAAGACCAAA AAACCTGATC CCAGAACATG TCAGCCGCAG 
GACCGTCCGC GCAGCGCTGT GGAGCAGATG AGCAGTGGTG CTGAAGTGGG CCGAACACGC 
ATGAGTGTGG AGGAGCAGAT GGAGAGAATC AGACGACACC AGCAGGGGGC GCTGAGAGAG 
CGCAGGAGAG AAGATGGCTC TCTATCCCGC AGCCTGTCCT TCACTAAGGA CAGCACATAC 
TACACACTGC AGGCACGTAA GAAGAGTCCG GTGATCTCTC CAGACGAGCA GGACGAGCGC 
AGAGACATGA CGCCTGTGGA GCGAGACACA AAACACACAA AACACCCTGA CGCAGAGGAA 
AACTGCAGAG ACACTAAGGA GCAGCTGCCA GCACACACCC ACCTGGAGAT TAAGGACAAA 
GCATCTGTGA CTCGCTCGGT CTCCATGAAA GAGTCTCTCC TGAAGAGTGT GAGTCCCAAA 
CCCGTGCTGA AGCAGGACGA ATCTGAGTGT AAACTGGAGT ACGACAGAAA GCTGGCCACT 
CAGAACAGCC TGCAGACGGC AGTGATGGTG CGTGTGGGCA CTGAGGAGGA TGAAGAGGAT 
GAAGAAGAGG AGCAGAAAGA CTCAGTTCAG CAGCAGGAGG ACTCTTATGA GCTCAGCAAC 
AGCAAACACA GCACACTGAT CTCAGCATCA TGTGACAGGA AGAACACATC ATCACCATCG 
TCATCATCAG ACTGGAAGTC TCGCCCCCTC CTTCATCAGC TGCCCAACAC AAACACAACA 
GCGTCCAGGC CTCAGTTCAA CAGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298277.1
CDS	43..4032
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009300002.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300002.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA 
GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC 
TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA 
AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA 
AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG 
AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT 
GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC 
CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG 
TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA 
CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGG CCCGGATTAC 
AGGCTGTATA AGAGTGAGCC GGAGCTGACC ACAGTAGCCG AAGTCGACGA GAGTAACGGT 
GAAGACAAAT CAGAGCAGAC AGCAGAGAAA GAGCCGCCCG GCGTCACTAA AGGTATAGTT 
TACCCGGTGG GAGTGGTCAG TCCCAGAACT AAATCACCCA TGCCCGAGTC CTCCACCATC 
GCCTCCTACG TCACCTTGAG GAAGACCAAA AAACCTGATC CCAGAACATG TCAGCCGCAG 
GACCGTCCGC GCAGCGCTGT GGAGCAGATG AGCAGTGGTG CTGAAGTGGG CCGAACACGC 
ATGAGTGTGG AGGAGCAGAT GGAGAGAATC AGACGACACC AGCAGGGGGC GCTGAGAGAG 
CGCAGGAGAG AAGATGGCTC TCTATCCCGC AGCCTGTCCT TCACTAAGGA CAGCACATAC 
TACACACTGC AGGCACGTAA GAAGAGTCCG GTGATCTCTC CAGACGAGCA GGACGAGCGC 
AGAGACATGA CGCCTGTGGA GCGAGACACA AAACACACAA AACACCCTGA CGCAGAGGAA 
AACTGCAGAG ACACTAAGGA GCAGCTGCCA GCACACACCC ACCTGGAGAT TAAGGACAAA 
GCATCTGTGA CTCGCTCGGT CTCCATGAAA GAGTCTCTCC TGAAGAGTGT GAGTCCCAAA 
CCCGTGCTGA AGCAGGACGA ATCTGAGTGT AAACTGGAGT ACGACAGAAA GCTGGCCACT 
CAGAACAGCC TGCAGACGGC AGTGATGGTG CGTGTGGGCA CTGAGGAGGA TGAAGAGGAT 
GAAGAAGAGG AGCAGAAAGA CTCAGTTCAG CAGCAGGAGG ACTCTTATGA GCTCAGCAAC 
AGCAAACACA GCACACTGAT CTCAGCATCA TGTGACAGGA AGAACACATC ATCACCATCG 
TCATCATCAG ACTGGAAGTC TCGCCCCCTC CTTCATCAGC TGCCCAACAC AAACACAACA 
GCGTCCAGGC CTCAGTTCAA CAGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300003.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298278.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X13, mRNA.

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CloneID	ODa45112
Clone ID Related Accession (Same CDS sequence)	XM_009300003.2 , XM_009300003.1
Accession Version	XM_009300003.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACAACCA 
GCGACCAAAG CACCAGAGGC GGAGAAGAAG GAGCGTCCCA CCAGCACCAT GAGCGAAGCC 
TCAAACTACA CCGGCGGCTC CGACTACACC ACACATCCCA GCAGCCCCAC AACCAGACCA 
TCCCGGTCCT CAAAGAAAGT GCACAACTTT GGGAAAAGGT CCAACTCCAT CAAGAGGAAC 
CCCAATGCAC CTGTGATCAA AAACGGCTGG CTTCACAAAC AGGACAGCAC TGGGATGAAG 
ATGTGGAAGA AGAGATGGTT CGTTCTGTCT GATATGTGCC TCTTCTACTA CAGAGATGAG 
AAGGAAGAGG GGATTCTGGG AAGCATCCTC CTGCCTAGCT TTCACATCTC CATGCTGTCC 
GTGGACGACC ATATTACCCG GAAATACGCC TTCAAGGCGA CGCATCCAAA CATGCGGACG 
TATTATTTCA GCACAGACAC AGCCAAAGAT ATGGAGTCCT GGATGAAAGT GATGTCGGAT 
GCGGCGATGG CTCACAGCGA GCCAATCAGA AGGCTTGAGA AGGTAAAGCT GGACTCTCGC 
GTTCCTCAGG AGATGAACAA CATGCTGAAC CACCGCGTCT TGACACGACC CGAGATCCAA 
AACAACGAGA GGAACCGCGA GACGCTCCGC CAAGAGGACA AGAAGAGCAA ACCATCAGAG 
AAACCTCAGA AAGACAAAGA CAGAGTCAGC ATGCAGAAAG ACGGAGAGCG CTTCAGTGAA 
AACTACACTC ACCGGCAGAA AGACGGCGAG CGCTACATGC TGCAGAAAGA CCTGGAGAAA 
TTTGTCCTGC AGAAAGACGG AGAGAAGTAC GCCGTGCAGA AAGAAGGCGA CAAATATCTC 
CTGCAGAAAG ACGGACACAA ATACACAGTG CACAAAGACG GAGACAAATA CACGCTTCAG 
AGAGAACTCG AGATGTACAA TCCACCAAAA GACGCGGAGA AGATCCCGCA GAAAGATGCA 
AGGTTTAGTC TGGTGCAGAA GGAGCTGGAG AAACCCGTCC CGCCACCGCG ATCTGAGGAA 
AAATACGGCT TTCAGAAAGA GGGCACCATT GAGCGTCCGC TAACTAAAAT AAATAGCATT 
AAACTGCAGC CGGCTCAAGC AGCCGCCATC GCTGCAGCCG TTTCATCCTC TAGGATGATG 
CAGAAACAGG TTAATGGCTC CGGGGAACGA GCGGGAGACA GAAGTCCTGG AGATTTGAGT 
ACTGTGAGGA CGAGCGTCCC GCCACCAACG CAGCCGGCTC CAGAACCAGA GCGAAGCCTC 
AGCAGAACCA GCTCAATGCA GCAGCTGGAG CAGTGGGTTC GCACACACCG CACACGAGCT 
CCAGACGACG ACACCAGGAG CATTACGTCC TACCAGACCC TGCCAAGGAA CATGCCGAGC 
CACCGGGCGC AGATTGTCCC TCGCTACCCT GAGGGCTACC GCACTCTGCC CCGAAACATG 
CTGCGTCCGG ACAGCATCTG CAGTGTAGCA GGATCAGTGT ACGACCGAGC GCTTCAGCCC 
AGCACGCAGG AAAAGCGGCG CTCCATCCGA GATGACACCA TGTGGCAGCT GTACGAGTGG 
CAGCAGCGGC AGGCGCACAG TCGGCTTGGG TACGGGACGC TGCCCAGTCC CAAAACTATG 
GGGCAGATCG CGGAGTCCAT CCCCACGTCC CCATCCCATG GCTCTCTGGC CGGATATCAC 
ACCTTCTCCC CGAACAGACC GCTCAACCCT GACTCGCGCT CGGAGCTGTC TTCGCCTGTG 
TTTCGTGGAG ACGTGACCAT CGAGCGACGC CACCGACCAC ACCTGTCCAA GTACTCCTAT 
CCTGCGGAAC GCAGAGCTGC TCCTCCCGCA CAGAACATCA CTGCACAGTC TCTTCAAGGC 
AAAACGCCAG AGGAACTGAC CCTGCTGCTC ATCAAACTGC GGCGGCAGCA GGCCGAGCTC 
AACAGCCTCC GTGAGCACAC ACTCGCGCAG CTCATGCAGC TGAACCTCGA CGCCACCAAC 
CCAAAGAGTG AAATCCTCTC TCATCACCTG CAGAGGAATC TCATGTACTT GGACAGTCAG 
ATGAAGGAGA ACGAACCCAT AATCTTCATG ATTCACACTA TGATTGAGAA CTCGGCGCCC 
CGGCCGCAGC TCTACCAGCA ACAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG 
TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA 
GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG 
ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC 
CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG 
CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC 
GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG 
ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT 
GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT 
GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT 
CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC 
CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC 
CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG 
ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG 
AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA 
ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC 
AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG 
ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA 
ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC 
CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT 
CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC 
ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG 
CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG 
AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG 
TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG 
GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT 
CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA 
TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC 
CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298278.1
CDS	74..4036
Translation	MLKFCTTSRSSHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009300003.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300003.2

ORF Insert Sequence:

ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACAACCA 
GCGACCAAAG CACCAGAGGC GGAGAAGAAG GAGCGTCCCA CCAGCACCAT GAGCGAAGCC 
TCAAACTACA CCGGCGGCTC CGACTACACC ACACATCCCA GCAGCCCCAC AACCAGACCA 
TCCCGGTCCT CAAAGAAAGT GCACAACTTT GGGAAAAGGT CCAACTCCAT CAAGAGGAAC 
CCCAATGCAC CTGTGATCAA AAACGGCTGG CTTCACAAAC AGGACAGCAC TGGGATGAAG 
ATGTGGAAGA AGAGATGGTT CGTTCTGTCT GATATGTGCC TCTTCTACTA CAGAGATGAG 
AAGGAAGAGG GGATTCTGGG AAGCATCCTC CTGCCTAGCT TTCACATCTC CATGCTGTCC 
GTGGACGACC ATATTACCCG GAAATACGCC TTCAAGGCGA CGCATCCAAA CATGCGGACG 
TATTATTTCA GCACAGACAC AGCCAAAGAT ATGGAGTCCT GGATGAAAGT GATGTCGGAT 
GCGGCGATGG CTCACAGCGA GCCAATCAGA AGGCTTGAGA AGGTAAAGCT GGACTCTCGC 
GTTCCTCAGG AGATGAACAA CATGCTGAAC CACCGCGTCT TGACACGACC CGAGATCCAA 
AACAACGAGA GGAACCGCGA GACGCTCCGC CAAGAGGACA AGAAGAGCAA ACCATCAGAG 
AAACCTCAGA AAGACAAAGA CAGAGTCAGC ATGCAGAAAG ACGGAGAGCG CTTCAGTGAA 
AACTACACTC ACCGGCAGAA AGACGGCGAG CGCTACATGC TGCAGAAAGA CCTGGAGAAA 
TTTGTCCTGC AGAAAGACGG AGAGAAGTAC GCCGTGCAGA AAGAAGGCGA CAAATATCTC 
CTGCAGAAAG ACGGACACAA ATACACAGTG CACAAAGACG GAGACAAATA CACGCTTCAG 
AGAGAACTCG AGATGTACAA TCCACCAAAA GACGCGGAGA AGATCCCGCA GAAAGATGCA 
AGGTTTAGTC TGGTGCAGAA GGAGCTGGAG AAACCCGTCC CGCCACCGCG ATCTGAGGAA 
AAATACGGCT TTCAGAAAGA GGGCACCATT GAGCGTCCGC TAACTAAAAT AAATAGCATT 
AAACTGCAGC CGGCTCAAGC AGCCGCCATC GCTGCAGCCG TTTCATCCTC TAGGATGATG 
CAGAAACAGG TTAATGGCTC CGGGGAACGA GCGGGAGACA GAAGTCCTGG AGATTTGAGT 
ACTGTGAGGA CGAGCGTCCC GCCACCAACG CAGCCGGCTC CAGAACCAGA GCGAAGCCTC 
AGCAGAACCA GCTCAATGCA GCAGCTGGAG CAGTGGGTTC GCACACACCG CACACGAGCT 
CCAGACGACG ACACCAGGAG CATTACGTCC TACCAGACCC TGCCAAGGAA CATGCCGAGC 
CACCGGGCGC AGATTGTCCC TCGCTACCCT GAGGGCTACC GCACTCTGCC CCGAAACATG 
CTGCGTCCGG ACAGCATCTG CAGTGTAGCA GGATCAGTGT ACGACCGAGC GCTTCAGCCC 
AGCACGCAGG AAAAGCGGCG CTCCATCCGA GATGACACCA TGTGGCAGCT GTACGAGTGG 
CAGCAGCGGC AGGCGCACAG TCGGCTTGGG TACGGGACGC TGCCCAGTCC CAAAACTATG 
GGGCAGATCG CGGAGTCCAT CCCCACGTCC CCATCCCATG GCTCTCTGGC CGGATATCAC 
ACCTTCTCCC CGAACAGACC GCTCAACCCT GACTCGCGCT CGGAGCTGTC TTCGCCTGTG 
TTTCGTGGAG ACGTGACCAT CGAGCGACGC CACCGACCAC ACCTGTCCAA GTACTCCTAT 
CCTGCGGAAC GCAGAGCTGC TCCTCCCGCA CAGAACATCA CTGCACAGTC TCTTCAAGGC 
AAAACGCCAG AGGAACTGAC CCTGCTGCTC ATCAAACTGC GGCGGCAGCA GGCCGAGCTC 
AACAGCCTCC GTGAGCACAC ACTCGCGCAG CTCATGCAGC TGAACCTCGA CGCCACCAAC 
CCAAAGAGTG AAATCCTCTC TCATCACCTG CAGAGGAATC TCATGTACTT GGACAGTCAG 
ATGAAGGAGA ACGAACCCAT AATCTTCATG ATTCACACTA TGATTGAGAA CTCGGCGCCC 
CGGCCGCAGC TCTACCAGCA ACAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG 
TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA 
GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG 
ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC 
CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG 
CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC 
GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG 
ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT 
GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT 
GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT 
CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC 
CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC 
CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG 
ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG 
AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA 
ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC 
AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG 
ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA 
ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC 
CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT 
CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC 
ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG 
CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG 
AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG 
TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG 
GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT 
CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA 
TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC 
CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300003.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298278.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa45112
Clone ID Related Accession (Same CDS sequence)	XM_009300003.2 , XM_009300003.1
Accession Version	XM_009300003.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACAACCA 
GCGACCAAAG CACCAGAGGC GGAGAAGAAG GAGCGTCCCA CCAGCACCAT GAGCGAAGCC 
TCAAACTACA CCGGCGGCTC CGACTACACC ACACATCCCA GCAGCCCCAC AACCAGACCA 
TCCCGGTCCT CAAAGAAAGT GCACAACTTT GGGAAAAGGT CCAACTCCAT CAAGAGGAAC 
CCCAATGCAC CTGTGATCAA AAACGGCTGG CTTCACAAAC AGGACAGCAC TGGGATGAAG 
ATGTGGAAGA AGAGATGGTT CGTTCTGTCT GATATGTGCC TCTTCTACTA CAGAGATGAG 
AAGGAAGAGG GGATTCTGGG AAGCATCCTC CTGCCTAGCT TTCACATCTC CATGCTGTCC 
GTGGACGACC ATATTACCCG GAAATACGCC TTCAAGGCGA CGCATCCAAA CATGCGGACG 
TATTATTTCA GCACAGACAC AGCCAAAGAT ATGGAGTCCT GGATGAAAGT GATGTCGGAT 
GCGGCGATGG CTCACAGCGA GCCAATCAGA AGGCTTGAGA AGGTAAAGCT GGACTCTCGC 
GTTCCTCAGG AGATGAACAA CATGCTGAAC CACCGCGTCT TGACACGACC CGAGATCCAA 
AACAACGAGA GGAACCGCGA GACGCTCCGC CAAGAGGACA AGAAGAGCAA ACCATCAGAG 
AAACCTCAGA AAGACAAAGA CAGAGTCAGC ATGCAGAAAG ACGGAGAGCG CTTCAGTGAA 
AACTACACTC ACCGGCAGAA AGACGGCGAG CGCTACATGC TGCAGAAAGA CCTGGAGAAA 
TTTGTCCTGC AGAAAGACGG AGAGAAGTAC GCCGTGCAGA AAGAAGGCGA CAAATATCTC 
CTGCAGAAAG ACGGACACAA ATACACAGTG CACAAAGACG GAGACAAATA CACGCTTCAG 
AGAGAACTCG AGATGTACAA TCCACCAAAA GACGCGGAGA AGATCCCGCA GAAAGATGCA 
AGGTTTAGTC TGGTGCAGAA GGAGCTGGAG AAACCCGTCC CGCCACCGCG ATCTGAGGAA 
AAATACGGCT TTCAGAAAGA GGGCACCATT GAGCGTCCGC TAACTAAAAT AAATAGCATT 
AAACTGCAGC CGGCTCAAGC AGCCGCCATC GCTGCAGCCG TTTCATCCTC TAGGATGATG 
CAGAAACAGG TTAATGGCTC CGGGGAACGA GCGGGAGACA GAAGTCCTGG AGATTTGAGT 
ACTGTGAGGA CGAGCGTCCC GCCACCAACG CAGCCGGCTC CAGAACCAGA GCGAAGCCTC 
AGCAGAACCA GCTCAATGCA GCAGCTGGAG CAGTGGGTTC GCACACACCG CACACGAGCT 
CCAGACGACG ACACCAGGAG CATTACGTCC TACCAGACCC TGCCAAGGAA CATGCCGAGC 
CACCGGGCGC AGATTGTCCC TCGCTACCCT GAGGGCTACC GCACTCTGCC CCGAAACATG 
CTGCGTCCGG ACAGCATCTG CAGTGTAGCA GGATCAGTGT ACGACCGAGC GCTTCAGCCC 
AGCACGCAGG AAAAGCGGCG CTCCATCCGA GATGACACCA TGTGGCAGCT GTACGAGTGG 
CAGCAGCGGC AGGCGCACAG TCGGCTTGGG TACGGGACGC TGCCCAGTCC CAAAACTATG 
GGGCAGATCG CGGAGTCCAT CCCCACGTCC CCATCCCATG GCTCTCTGGC CGGATATCAC 
ACCTTCTCCC CGAACAGACC GCTCAACCCT GACTCGCGCT CGGAGCTGTC TTCGCCTGTG 
TTTCGTGGAG ACGTGACCAT CGAGCGACGC CACCGACCAC ACCTGTCCAA GTACTCCTAT 
CCTGCGGAAC GCAGAGCTGC TCCTCCCGCA CAGAACATCA CTGCACAGTC TCTTCAAGGC 
AAAACGCCAG AGGAACTGAC CCTGCTGCTC ATCAAACTGC GGCGGCAGCA GGCCGAGCTC 
AACAGCCTCC GTGAGCACAC ACTCGCGCAG CTCATGCAGC TGAACCTCGA CGCCACCAAC 
CCAAAGAGTG AAATCCTCTC TCATCACCTG CAGAGGAATC TCATGTACTT GGACAGTCAG 
ATGAAGGAGA ACGAACCCAT AATCTTCATG ATTCACACTA TGATTGAGAA CTCGGCGCCC 
CGGCCGCAGC TCTACCAGCA ACAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG 
TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA 
GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG 
ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC 
CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG 
CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC 
GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG 
ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT 
GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT 
GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT 
CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC 
CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC 
CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG 
ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG 
AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA 
ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC 
AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG 
ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA 
ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC 
CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT 
CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC 
ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG 
CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG 
AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG 
TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG 
GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT 
CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA 
TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC 
CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298278.1
CDS	33..3995
Translation	MLKFCTTSRSSHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009300003.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300003.1

ORF Insert Sequence:

ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACAACCA 
GCGACCAAAG CACCAGAGGC GGAGAAGAAG GAGCGTCCCA CCAGCACCAT GAGCGAAGCC 
TCAAACTACA CCGGCGGCTC CGACTACACC ACACATCCCA GCAGCCCCAC AACCAGACCA 
TCCCGGTCCT CAAAGAAAGT GCACAACTTT GGGAAAAGGT CCAACTCCAT CAAGAGGAAC 
CCCAATGCAC CTGTGATCAA AAACGGCTGG CTTCACAAAC AGGACAGCAC TGGGATGAAG 
ATGTGGAAGA AGAGATGGTT CGTTCTGTCT GATATGTGCC TCTTCTACTA CAGAGATGAG 
AAGGAAGAGG GGATTCTGGG AAGCATCCTC CTGCCTAGCT TTCACATCTC CATGCTGTCC 
GTGGACGACC ATATTACCCG GAAATACGCC TTCAAGGCGA CGCATCCAAA CATGCGGACG 
TATTATTTCA GCACAGACAC AGCCAAAGAT ATGGAGTCCT GGATGAAAGT GATGTCGGAT 
GCGGCGATGG CTCACAGCGA GCCAATCAGA AGGCTTGAGA AGGTAAAGCT GGACTCTCGC 
GTTCCTCAGG AGATGAACAA CATGCTGAAC CACCGCGTCT TGACACGACC CGAGATCCAA 
AACAACGAGA GGAACCGCGA GACGCTCCGC CAAGAGGACA AGAAGAGCAA ACCATCAGAG 
AAACCTCAGA AAGACAAAGA CAGAGTCAGC ATGCAGAAAG ACGGAGAGCG CTTCAGTGAA 
AACTACACTC ACCGGCAGAA AGACGGCGAG CGCTACATGC TGCAGAAAGA CCTGGAGAAA 
TTTGTCCTGC AGAAAGACGG AGAGAAGTAC GCCGTGCAGA AAGAAGGCGA CAAATATCTC 
CTGCAGAAAG ACGGACACAA ATACACAGTG CACAAAGACG GAGACAAATA CACGCTTCAG 
AGAGAACTCG AGATGTACAA TCCACCAAAA GACGCGGAGA AGATCCCGCA GAAAGATGCA 
AGGTTTAGTC TGGTGCAGAA GGAGCTGGAG AAACCCGTCC CGCCACCGCG ATCTGAGGAA 
AAATACGGCT TTCAGAAAGA GGGCACCATT GAGCGTCCGC TAACTAAAAT AAATAGCATT 
AAACTGCAGC CGGCTCAAGC AGCCGCCATC GCTGCAGCCG TTTCATCCTC TAGGATGATG 
CAGAAACAGG TTAATGGCTC CGGGGAACGA GCGGGAGACA GAAGTCCTGG AGATTTGAGT 
ACTGTGAGGA CGAGCGTCCC GCCACCAACG CAGCCGGCTC CAGAACCAGA GCGAAGCCTC 
AGCAGAACCA GCTCAATGCA GCAGCTGGAG CAGTGGGTTC GCACACACCG CACACGAGCT 
CCAGACGACG ACACCAGGAG CATTACGTCC TACCAGACCC TGCCAAGGAA CATGCCGAGC 
CACCGGGCGC AGATTGTCCC TCGCTACCCT GAGGGCTACC GCACTCTGCC CCGAAACATG 
CTGCGTCCGG ACAGCATCTG CAGTGTAGCA GGATCAGTGT ACGACCGAGC GCTTCAGCCC 
AGCACGCAGG AAAAGCGGCG CTCCATCCGA GATGACACCA TGTGGCAGCT GTACGAGTGG 
CAGCAGCGGC AGGCGCACAG TCGGCTTGGG TACGGGACGC TGCCCAGTCC CAAAACTATG 
GGGCAGATCG CGGAGTCCAT CCCCACGTCC CCATCCCATG GCTCTCTGGC CGGATATCAC 
ACCTTCTCCC CGAACAGACC GCTCAACCCT GACTCGCGCT CGGAGCTGTC TTCGCCTGTG 
TTTCGTGGAG ACGTGACCAT CGAGCGACGC CACCGACCAC ACCTGTCCAA GTACTCCTAT 
CCTGCGGAAC GCAGAGCTGC TCCTCCCGCA CAGAACATCA CTGCACAGTC TCTTCAAGGC 
AAAACGCCAG AGGAACTGAC CCTGCTGCTC ATCAAACTGC GGCGGCAGCA GGCCGAGCTC 
AACAGCCTCC GTGAGCACAC ACTCGCGCAG CTCATGCAGC TGAACCTCGA CGCCACCAAC 
CCAAAGAGTG AAATCCTCTC TCATCACCTG CAGAGGAATC TCATGTACTT GGACAGTCAG 
ATGAAGGAGA ACGAACCCAT AATCTTCATG ATTCACACTA TGATTGAGAA CTCGGCGCCC 
CGGCCGCAGC TCTACCAGCA ACAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG 
TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA 
GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG 
ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC 
CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG 
CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC 
GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG 
ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT 
GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT 
GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT 
CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC 
CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC 
CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG 
ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG 
AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA 
ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC 
AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG 
ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA 
ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC 
CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT 
CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC 
ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG 
CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG 
AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG 
TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG 
GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT 
CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA 
TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC 
CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300004.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298279.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa45113
Clone ID Related Accession (Same CDS sequence)	XM_009300004.2
Accession Version	XM_009300004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAGA TGTGCTGCCA CAATGAGCGA AAGGTGACCT GCAAGCATCC CGTCAGCGGC 
GTCCCGTCAC AAGACAACTG CATCTTTGTG GTCAACGAAC AACCAGCGAC CAAAGCACCA 
GAGGCGGAGA AGAAGGAGCG TCCCACCAGC ACCATGAGCG AAGCCTCAAA CTACACCGGC 
GGCTCCGACT ACACCACACA TCCCAGCAGC CCCACAACCA GACCATCCCG GTCCTCAAAG 
AAAGTGCACA ACTTTGGGAA AAGGTCCAAC TCCATCAAGA GGAACCCCAA TGCACCTGTG 
ATCAAAAACG GCTGGCTTCA CAAACAGGAC AGCACTGGGA TGAAGATGTG GAAGAAGAGA 
TGGTTCGTTC TGTCTGATAT GTGCCTCTTC TACTACAGAG ATGAGAAGGA AGAGGGGATT 
CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC ATCTCCATGC TGTCCGTGGA CGACCATATT 
ACCCGGAAAT ACGCCTTCAA GGCGACGCAT CCAAACATGC GGACGTATTA TTTCAGCACA 
GACACAGCCA AAGATATGGA GTCCTGGATG AAAGTGATGT CGGATGCGGC GATGGCTCAC 
AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA AAGCTGGACT CTCGCGTTCC TCAGGAGATG 
AACAACATGC TGAACCACCG CGTCTTGACA CGACCCGAGA TCCAAAACAA CGAGAGGAAC 
CGCGAGACGC TCCGCCAAGA GGACAAGAAG AGCAAACCAT CAGAGAAACC TCAGAAAGAC 
AAAGACAGAG TCAGCATGCA GAAAGACGGA GAGCGCTTCA GTGAAAACTA CACTCACCGG 
CAGAAAGACG GCGAGCGCTA CATGCTGCAG AAAGACCTGG AGAAATTTGT CCTGCAGAAA 
GACGGAGAGA AGTACGCCGT GCAGAAAGAA GGCGACAAAT ATCTCCTGCA GAAAGACGGA 
CACAAATACA CAGTGCACAA AGACGGAGAC AAATACACGC TTCAGAGAGA ACTCGAGATG 
TACAATCCAC CAAAAGACGC GGAGAAGATC CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG 
CAGAAGGAGC TGGAGAAACC CGTCCCGCCA CCGCGATCTG AGGAAAAATA CGGCTTTCAG 
AAAGAGGGCA CCATTGAGCG TCCGCTAACT AAAATAAATA GCATTAAACT GCAGCCGGCT 
CAAGCAGCCG CCATCGCTGC AGCCGTTTCA TCCTCTAGGA TGATGCAGAA ACAGGTTAAT 
GGCTCCGGGG AACGAGCGGG AGACAGAAGT CCTGGAGATT TGAGTACTGT GAGGACGAGC 
GTCCCGCCAC CAACGCAGCC GGCTCCAGAA CCAGAGCGAA GCCTCAGCAG AACCAGCTCA 
ATGCAGCAGC TGGAGCAGTG GGTTCGCACA CACCGCACAC GAGCTCCAGA CGACGACACC 
AGGAGCATTA CGTCCTACCA GACCCTGCCA AGGAACATGC CGAGCCACCG GGCGCAGATT 
GTCCCTCGCT ACCCTGAGGG CTACCGCACT CTGCCCCGAA ACATGCTGCG TCCGGACAGC 
ATCTGCAGTG TAGCAGGATC AGTGTACGAC CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG 
CGGCGCTCCA TCCGAGATGA CACCATGTGG CAGCTGTACG AGTGGCAGCA GCGGCAGGCG 
CACAGTCGGC TTGGGTACGG GACGCTGCCC AGTCCCAAAA CTATGGGGCA GATCGCGGAG 
TCCATCCCCA CGTCCCCATC CCATGGCTCT CTGGCCGGAT ATCACACCTT CTCCCCGAAC 
AGACCGCTCA ACCCTGACTC GCGCTCGGAG CTGTCTTCGC CTGTGTTTCG TGGAGACGTG 
ACCATCGAGC GACGCCACCG ACCACACCTG TCCAAGTACT CCTATCCTGC GGAACGCAGA 
GCTGCTCCTC CCGCACAGAA CATCACTGCA CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA 
CTGACCCTGC TGCTCATCAA ACTGCGGCGG CAGCAGGCCG AGCTCAACAG CCTCCGTGAG 
CACACACTCG CGCAGCTCAT GCAGCTGAAC CTCGACGCCA CCAACCCAAA GAGTGAAATC 
CTCTCTCATC ACCTGCAGAG GAATCTCATG TACTTGGACA GTCAGATGAA GGAGAACGAA 
CCCATAATCT TCATGATTCA CACTATGATT GAGAACTCGG CGCCCCGGCC GCAGCTCTAC 
CAGCAACAGA TGAGTCCTGA AGAGTACAGA GAAAACACTT ACATGTACAG GCCGGAGGAG 
CTCGATGTCG ACGCGAAGTT AAGTCGGCTC TGTGAGCAGG ACAAAGTAGT GCGAACTCAA 
GAAGAGAAAC TGCAGCAGCT CCACAGAGAA AAGCACACAC TGGAGACGGC GCTGCTCTCC 
GCCAGTCAGG AGATCGAGAT GAGCTCTGAA AACCCAGCGG CGGTCCAGAG CGTGGTCCAG 
CAGAGGGACG TCCTGCAGAG TGGCCTGCTG AGCACCTGCA GAGAGCTGAG CCGCGTCAGC 
ACTGAGCTGG AGCGAAGCTG GAGAGAATAT GATCGACTAG AGGCCGATGT TACACTTGCC 
AAAACTAACC TGCTGGAGCA GCTGGAGGCC CTCGGCAGCC CACAGACGGA GCCGCCCAGT 
CAAAAGCATG TTCAGATCCA GAAAGAGTTG TGGAGAATTC AGGATGTGAT GGAGGCGCTG 
AGCAAGAACA AACCCAAGAG AAACCCTGAA CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA 
ACCCTGCATA AAAGTGAAGA CTCTGTAGAC TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG 
CCGTACTCAT ATGAAGGAGG CGATCGACCA CCGCCCCTCC CCAACCCCGG TCAACCGCAC 
ACCACCCGCA CAACACCACA CCGACCAGAG GACCGCAAAA CTGCCCAACG CAACGGCACG 
CACACCGGCC CGGATTACAG GCTGTATAAG AGTGAGCCGG AGCTGACCAC AGTAGCCGAA 
GTCGACGAGA GTAACGGTGA AGACAAATCA GAGCAGACAG CAGAGAAAGA GCCGCCCGGC 
GTCACTAAAG GTATAGTTTA CCCGGTGGGA GTGGTCAGTC CCAGAACTAA ATCACCCATG 
CCCGAGTCCT CCACCATCGC CTCCTACGTC ACCTTGAGGA AGACCAAAAA ACCTGATCCC 
AGAACATGTC AGCCGCAGGA CCGTCCGCGC AGCGCTGTGG AGCAGATGAG CAGTGGTGCT 
GAAGTGGGCC GAACACGCAT GAGTGTGGAG GAGCAGATGG AGAGAATCAG ACGACACCAG 
CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA GATGGCTCTC TATCCCGCAG CCTGTCCTTC 
ACTAAGGACA GCACATACTA CACACTGCAG GCACGTAAGA AGAGTCCGGT GATCTCTCCA 
GACGAGCAGG ACGAGCGCAG AGACATGACG CCTGTGGAGC GAGACACAAA ACACACAAAA 
CACCCTGACG CAGAGGAAAA CTGCAGAGAC ACTAAGGAGC AGCTGCCAGC ACACACCCAC 
CTGGAGATTA AGGACAAAGC ATCTGTGACT CGCTCGGTCT CCATGAAAGA GTCTCTCCTG 
AAGAGTGTGA GTCCCAAACC CGTGCTGAAG CAGGACGAAT CTGAGTGTAA ACTGGAGTAC 
GACAGAAAGC TGGCCACTCA GAACAGCCTG CAGACGGCAG TGATGGTGCG TGTGGGCACT 
GAGGAGGATG AAGAGGATGA AGAAGAGGAG CAGAAAGACT CAGTTCAGCA GCAGGAGGAC 
TCTTATGAGC TCAGCAACAG CAAACACAGC ACACTGATCT CAGCATCATG TGACAGGAAG 
AACACATCAT CACCATCGTC ATCATCAGAC TGGAAGTCTC GCCCCCTCCT TCATCAGCTG 
CCCAACACAA ACACAACAGC GTCCAGGCCT CAGTTCAACA GCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298279.1
CDS	123..4070
Translation	MKQMCCHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009300004.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300004.2

ORF Insert Sequence:

ATGAAGCAGA TGTGCTGCCA CAATGAGCGA AAGGTGACCT GCAAGCATCC CGTCAGCGGC 
GTCCCGTCAC AAGACAACTG CATCTTTGTG GTCAACGAAC AACCAGCGAC CAAAGCACCA 
GAGGCGGAGA AGAAGGAGCG TCCCACCAGC ACCATGAGCG AAGCCTCAAA CTACACCGGC 
GGCTCCGACT ACACCACACA TCCCAGCAGC CCCACAACCA GACCATCCCG GTCCTCAAAG 
AAAGTGCACA ACTTTGGGAA AAGGTCCAAC TCCATCAAGA GGAACCCCAA TGCACCTGTG 
ATCAAAAACG GCTGGCTTCA CAAACAGGAC AGCACTGGGA TGAAGATGTG GAAGAAGAGA 
TGGTTCGTTC TGTCTGATAT GTGCCTCTTC TACTACAGAG ATGAGAAGGA AGAGGGGATT 
CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC ATCTCCATGC TGTCCGTGGA CGACCATATT 
ACCCGGAAAT ACGCCTTCAA GGCGACGCAT CCAAACATGC GGACGTATTA TTTCAGCACA 
GACACAGCCA AAGATATGGA GTCCTGGATG AAAGTGATGT CGGATGCGGC GATGGCTCAC 
AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA AAGCTGGACT CTCGCGTTCC TCAGGAGATG 
AACAACATGC TGAACCACCG CGTCTTGACA CGACCCGAGA TCCAAAACAA CGAGAGGAAC 
CGCGAGACGC TCCGCCAAGA GGACAAGAAG AGCAAACCAT CAGAGAAACC TCAGAAAGAC 
AAAGACAGAG TCAGCATGCA GAAAGACGGA GAGCGCTTCA GTGAAAACTA CACTCACCGG 
CAGAAAGACG GCGAGCGCTA CATGCTGCAG AAAGACCTGG AGAAATTTGT CCTGCAGAAA 
GACGGAGAGA AGTACGCCGT GCAGAAAGAA GGCGACAAAT ATCTCCTGCA GAAAGACGGA 
CACAAATACA CAGTGCACAA AGACGGAGAC AAATACACGC TTCAGAGAGA ACTCGAGATG 
TACAATCCAC CAAAAGACGC GGAGAAGATC CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG 
CAGAAGGAGC TGGAGAAACC CGTCCCGCCA CCGCGATCTG AGGAAAAATA CGGCTTTCAG 
AAAGAGGGCA CCATTGAGCG TCCGCTAACT AAAATAAATA GCATTAAACT GCAGCCGGCT 
CAAGCAGCCG CCATCGCTGC AGCCGTTTCA TCCTCTAGGA TGATGCAGAA ACAGGTTAAT 
GGCTCCGGGG AACGAGCGGG AGACAGAAGT CCTGGAGATT TGAGTACTGT GAGGACGAGC 
GTCCCGCCAC CAACGCAGCC GGCTCCAGAA CCAGAGCGAA GCCTCAGCAG AACCAGCTCA 
ATGCAGCAGC TGGAGCAGTG GGTTCGCACA CACCGCACAC GAGCTCCAGA CGACGACACC 
AGGAGCATTA CGTCCTACCA GACCCTGCCA AGGAACATGC CGAGCCACCG GGCGCAGATT 
GTCCCTCGCT ACCCTGAGGG CTACCGCACT CTGCCCCGAA ACATGCTGCG TCCGGACAGC 
ATCTGCAGTG TAGCAGGATC AGTGTACGAC CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG 
CGGCGCTCCA TCCGAGATGA CACCATGTGG CAGCTGTACG AGTGGCAGCA GCGGCAGGCG 
CACAGTCGGC TTGGGTACGG GACGCTGCCC AGTCCCAAAA CTATGGGGCA GATCGCGGAG 
TCCATCCCCA CGTCCCCATC CCATGGCTCT CTGGCCGGAT ATCACACCTT CTCCCCGAAC 
AGACCGCTCA ACCCTGACTC GCGCTCGGAG CTGTCTTCGC CTGTGTTTCG TGGAGACGTG 
ACCATCGAGC GACGCCACCG ACCACACCTG TCCAAGTACT CCTATCCTGC GGAACGCAGA 
GCTGCTCCTC CCGCACAGAA CATCACTGCA CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA 
CTGACCCTGC TGCTCATCAA ACTGCGGCGG CAGCAGGCCG AGCTCAACAG CCTCCGTGAG 
CACACACTCG CGCAGCTCAT GCAGCTGAAC CTCGACGCCA CCAACCCAAA GAGTGAAATC 
CTCTCTCATC ACCTGCAGAG GAATCTCATG TACTTGGACA GTCAGATGAA GGAGAACGAA 
CCCATAATCT TCATGATTCA CACTATGATT GAGAACTCGG CGCCCCGGCC GCAGCTCTAC 
CAGCAACAGA TGAGTCCTGA AGAGTACAGA GAAAACACTT ACATGTACAG GCCGGAGGAG 
CTCGATGTCG ACGCGAAGTT AAGTCGGCTC TGTGAGCAGG ACAAAGTAGT GCGAACTCAA 
GAAGAGAAAC TGCAGCAGCT CCACAGAGAA AAGCACACAC TGGAGACGGC GCTGCTCTCC 
GCCAGTCAGG AGATCGAGAT GAGCTCTGAA AACCCAGCGG CGGTCCAGAG CGTGGTCCAG 
CAGAGGGACG TCCTGCAGAG TGGCCTGCTG AGCACCTGCA GAGAGCTGAG CCGCGTCAGC 
ACTGAGCTGG AGCGAAGCTG GAGAGAATAT GATCGACTAG AGGCCGATGT TACACTTGCC 
AAAACTAACC TGCTGGAGCA GCTGGAGGCC CTCGGCAGCC CACAGACGGA GCCGCCCAGT 
CAAAAGCATG TTCAGATCCA GAAAGAGTTG TGGAGAATTC AGGATGTGAT GGAGGCGCTG 
AGCAAGAACA AACCCAAGAG AAACCCTGAA CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA 
ACCCTGCATA AAAGTGAAGA CTCTGTAGAC TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG 
CCGTACTCAT ATGAAGGAGG CGATCGACCA CCGCCCCTCC CCAACCCCGG TCAACCGCAC 
ACCACCCGCA CAACACCACA CCGACCAGAG GACCGCAAAA CTGCCCAACG CAACGGCACG 
CACACCGGCC CGGATTACAG GCTGTATAAG AGTGAGCCGG AGCTGACCAC AGTAGCCGAA 
GTCGACGAGA GTAACGGTGA AGACAAATCA GAGCAGACAG CAGAGAAAGA GCCGCCCGGC 
GTCACTAAAG GTATAGTTTA CCCGGTGGGA GTGGTCAGTC CCAGAACTAA ATCACCCATG 
CCCGAGTCCT CCACCATCGC CTCCTACGTC ACCTTGAGGA AGACCAAAAA ACCTGATCCC 
AGAACATGTC AGCCGCAGGA CCGTCCGCGC AGCGCTGTGG AGCAGATGAG CAGTGGTGCT 
GAAGTGGGCC GAACACGCAT GAGTGTGGAG GAGCAGATGG AGAGAATCAG ACGACACCAG 
CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA GATGGCTCTC TATCCCGCAG CCTGTCCTTC 
ACTAAGGACA GCACATACTA CACACTGCAG GCACGTAAGA AGAGTCCGGT GATCTCTCCA 
GACGAGCAGG ACGAGCGCAG AGACATGACG CCTGTGGAGC GAGACACAAA ACACACAAAA 
CACCCTGACG CAGAGGAAAA CTGCAGAGAC ACTAAGGAGC AGCTGCCAGC ACACACCCAC 
CTGGAGATTA AGGACAAAGC ATCTGTGACT CGCTCGGTCT CCATGAAAGA GTCTCTCCTG 
AAGAGTGTGA GTCCCAAACC CGTGCTGAAG CAGGACGAAT CTGAGTGTAA ACTGGAGTAC 
GACAGAAAGC TGGCCACTCA GAACAGCCTG CAGACGGCAG TGATGGTGCG TGTGGGCACT 
GAGGAGGATG AAGAGGATGA AGAAGAGGAG CAGAAAGACT CAGTTCAGCA GCAGGAGGAC 
TCTTATGAGC TCAGCAACAG CAAACACAGC ACACTGATCT CAGCATCATG TGACAGGAAG 
AACACATCAT CACCATCGTC ATCATCAGAC TGGAAGTCTC GCCCCCTCCT TCATCAGCTG 
CCCAACACAA ACACAACAGC GTCCAGGCCT CAGTTCAACA GCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300005.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298280.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X9, mRNA.

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CloneID	ODa45114
Clone ID Related Accession (Same CDS sequence)	XM_009300005.2
Accession Version	XM_009300005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG 
CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA 
GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC 
AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG 
ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG 
CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG 
AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA 
TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG 
AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA 
GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG 
CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC 
ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT 
GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG 
ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA 
GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA 
GCATCATGTG ACAGGAAGAA CACATCATCA CCATCGTCAT CATCAGACTG GAAGTCTCGC 
CCCCTCCTTC ATCAGCTGCC CAACACAAAC ACAACAGCGT CCAGGCCTCA GTTCAACAGC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298280.1
CDS	43..3948
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009300005.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300005.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG 
CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA 
GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC 
AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG 
ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG 
CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG 
AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA 
TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG 
AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA 
GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG 
CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC 
ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT 
GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG 
ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA 
GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA 
GCATCATGTG ACAGGAAGAA CACATCATCA CCATCGTCAT CATCAGACTG GAAGTCTCGC 
CCCCTCCTTC ATCAGCTGCC CAACACAAAC ACAACAGCGT CCAGGCCTCA GTTCAACAGC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300006.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298281.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa45115
Clone ID Related Accession (Same CDS sequence)	XM_009300006.2
Accession Version	XM_009300006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG 
TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA 
GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG 
ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC 
CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG 
CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC 
GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG 
ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT 
GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT 
GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT 
CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC 
CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC 
CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG 
ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG 
AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA 
ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC 
AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG 
ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA 
ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC 
CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT 
CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC 
ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG 
CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG 
AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG 
TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG 
GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT 
CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA 
TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC 
CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298281.1
CDS	43..3945
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009300006.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300006.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG 
TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA 
GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG 
ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC 
CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG 
CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC 
GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG 
ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT 
GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT 
GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT 
CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC 
CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC 
CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG 
ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG 
AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA 
ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC 
AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG 
ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA 
ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC 
CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT 
CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC 
ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG 
CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG 
AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG 
TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG 
GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT 
CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA 
TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC 
CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300008.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298283.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa45116
Clone ID Related Accession (Same CDS sequence)	XM_009300008.2
Accession Version	XM_009300008.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC 
AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA 
GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG 
GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG 
AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG 
AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT 
GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG 
GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG 
ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA 
AACCCTCTGT CAACCCTGCA TAAAAGTGAA GACTCTGTAG ACTCCTCGTG TGTTCCTCCT 
CGTCCTCCTC TGCCGTACTC ATATGAAGGA GGCGATCGAC CACCGCCCCT CCCCAACCCC 
GGTCAACCGC ACACCACCCG CACAACACCA CACCGACCAG AGGACCGCAA AACTGCCCAA 
CGCAACGGCA CGCACACCGG CCCGGATTAC AGGCTGTATA AGAGTGAGCC GGAGCTGACC 
ACAGTAGCCG AAGTCGACGA GAGTAACGGT GAAGACAAAT CAGAGCAGAC AGCAGAGAAA 
GAGCCGCCCG GCGTCACTAA AGGTATAGTT TACCCGGTGG GAGTGGTCAG TCCCAGAACT 
AAATCACCCA TGCCCGAGTC CTCCACCATC GCCTCCTACG TCACCTTGAG GAAGACCAAA 
AAACCTGATC CCAGAACATG TCAGCCGCAG GACCGTCCGC GCAGCGCTGT GGAGCAGATG 
AGCAGTGGTG CTGAAGTGGG CCGAACACGC ATGAGTGTGG AGGAGCAGAT GGAGAGAATC 
AGACGACACC AGCAGGGGGC GCTGAGAGAG CGCAGGAGAG AAGATGGCTC TCTATCCCGC 
AGCCTGTCCT TCACTAAGGA CAGCACATAC TACACACTGC AGGCACGTAA GAAGAGTCCG 
GTGATCTCTC CAGACGAGCA GGACGAGCGC AGAGACATGA CGCCTGTGGA GCGAGACACA 
AAACACACAA AACACCCTGA CGCAGAGGAA AACTGCAGAG ACACTAAGGA GCAGCTGCCA 
GCACACACCC ACCTGGAGAT TAAGGACAAA GCATCTGTGA CTCGCTCGGT CTCCATGAAA 
GAGTCTCTCC TGAAGAGTGT GAGTCCCAAA CCCGTGCTGA AGCAGGACGA ATCTGAGTGT 
AAACTGGAGT ACGACAGAAA GCTGGCCACT CAGAACAGCC TGCAGACGGC AGTGATGGTG 
CGTGTGGGCA CTGAGGAGGA TGAAGAGGAT GAAGAAGAGG AGCAGAAAGA CTCAGTTCAG 
CAGCAGGAGG ACTCTTATGA GCTCAGCAAC AGCAAACACA GCACACTGAT CTCAGCATCA 
TGTGACAGGA AGAACACATC ATCACCATCG TCATCATCAG ACTGGAAGTC TCGCCCCCTC 
CTTCATCAGC TGCCCAACAC AAACACAACA GCGTCCAGGC CTCAGTTCAA CAGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298283.1
CDS	43..3942
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009300008.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300008.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC 
AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA 
GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG 
GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG 
AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG 
AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT 
GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG 
GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG 
ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA 
AACCCTCTGT CAACCCTGCA TAAAAGTGAA GACTCTGTAG ACTCCTCGTG TGTTCCTCCT 
CGTCCTCCTC TGCCGTACTC ATATGAAGGA GGCGATCGAC CACCGCCCCT CCCCAACCCC 
GGTCAACCGC ACACCACCCG CACAACACCA CACCGACCAG AGGACCGCAA AACTGCCCAA 
CGCAACGGCA CGCACACCGG CCCGGATTAC AGGCTGTATA AGAGTGAGCC GGAGCTGACC 
ACAGTAGCCG AAGTCGACGA GAGTAACGGT GAAGACAAAT CAGAGCAGAC AGCAGAGAAA 
GAGCCGCCCG GCGTCACTAA AGGTATAGTT TACCCGGTGG GAGTGGTCAG TCCCAGAACT 
AAATCACCCA TGCCCGAGTC CTCCACCATC GCCTCCTACG TCACCTTGAG GAAGACCAAA 
AAACCTGATC CCAGAACATG TCAGCCGCAG GACCGTCCGC GCAGCGCTGT GGAGCAGATG 
AGCAGTGGTG CTGAAGTGGG CCGAACACGC ATGAGTGTGG AGGAGCAGAT GGAGAGAATC 
AGACGACACC AGCAGGGGGC GCTGAGAGAG CGCAGGAGAG AAGATGGCTC TCTATCCCGC 
AGCCTGTCCT TCACTAAGGA CAGCACATAC TACACACTGC AGGCACGTAA GAAGAGTCCG 
GTGATCTCTC CAGACGAGCA GGACGAGCGC AGAGACATGA CGCCTGTGGA GCGAGACACA 
AAACACACAA AACACCCTGA CGCAGAGGAA AACTGCAGAG ACACTAAGGA GCAGCTGCCA 
GCACACACCC ACCTGGAGAT TAAGGACAAA GCATCTGTGA CTCGCTCGGT CTCCATGAAA 
GAGTCTCTCC TGAAGAGTGT GAGTCCCAAA CCCGTGCTGA AGCAGGACGA ATCTGAGTGT 
AAACTGGAGT ACGACAGAAA GCTGGCCACT CAGAACAGCC TGCAGACGGC AGTGATGGTG 
CGTGTGGGCA CTGAGGAGGA TGAAGAGGAT GAAGAAGAGG AGCAGAAAGA CTCAGTTCAG 
CAGCAGGAGG ACTCTTATGA GCTCAGCAAC AGCAAACACA GCACACTGAT CTCAGCATCA 
TGTGACAGGA AGAACACATC ATCACCATCG TCATCATCAG ACTGGAAGTC TCGCCCCCTC 
CTTCATCAGC TGCCCAACAC AAACACAACA GCGTCCAGGC CTCAGTTCAA CAGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300009.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298284.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X13, mRNA.

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CloneID	ODa45117
Clone ID Related Accession (Same CDS sequence)	XM_009300009.2
Accession Version	XM_009300009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC 
AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA 
GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG 
GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG 
AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG 
AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT 
GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG 
GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG 
ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA 
AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG 
CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG 
ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC 
AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG 
AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT 
GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG 
ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC 
TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT 
AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG 
GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG 
GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG 
GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC 
GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG 
GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA 
GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG 
ATCTCAGCAT CATGTGACAG GAAGAACACA TCATCACCAT CGTCATCATC AGACTGGAAG 
TCTCGCCCCC TCCTTCATCA GCTGCCCAAC ACAAACACAA CAGCGTCCAG GCCTCAGTTC 
AACAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298284.1
CDS	43..3774
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISASCDRKNTSSPSSSSDWKSRPLLHQLPNTNTTASRPQFNSD

Target ORF information:

RefSeq Version	XM_009300009.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300009.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC 
AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA 
GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG 
GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG 
AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG 
AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT 
GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG 
GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG 
ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA 
AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG 
CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG 
ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC 
AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG 
AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT 
GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG 
ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC 
TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT 
AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG 
GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG 
GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG 
GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC 
GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG 
GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA 
GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG 
ATCTCAGCAT CATGTGACAG GAAGAACACA TCATCACCAT CGTCATCATC AGACTGGAAG 
TCTCGCCCCC TCCTTCATCA GCTGCCCAAC ACAAACACAA CAGCGTCCAG GCCTCAGTTC 
AACAGCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300010.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298285.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa45118
Clone ID Related Accession (Same CDS sequence)	XM_009300010.2 , XM_009300010.3
Accession Version	XM_009300010.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG 
CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA 
GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC 
AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG 
ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG 
CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG 
AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA 
TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG 
AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA 
GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG 
CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC 
ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT 
GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG 
ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA 
GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA 
GTGAACAGTG TGTCGACTCC TCCTCAATCT CCAAGCTCCT CCTCCTCTCC TCCGCCTCCA 
CCCTCCCCTC CACAGCTGAC GGACGGGTCA CACTTCATGT GTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298285.1
CDS	43..3930
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISVNSVSTPPQSPSSSSSPPPPPSPPQLTDGSHFMCV

Target ORF information:

RefSeq Version	XM_009300010.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300010.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG 
CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA 
GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC 
AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG 
ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG 
CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG 
AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA 
TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG 
AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA 
GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG 
CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC 
ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT 
GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG 
ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA 
GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA 
GTGAACAGTG TGTCGACTCC TCCTCAATCT CCAAGCTCCT CCTCCTCTCC TCCGCCTCCA 
CCCTCCCCTC CACAGCTGAC GGACGGGTCA CACTTCATGT GTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300010.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298285.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X21, mRNA.

Price & Availability↑

CloneID	ODa45118
Clone ID Related Accession (Same CDS sequence)	XM_009300010.2 , XM_009300010.3
Accession Version	XM_009300010.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG 
CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA 
GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC 
AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG 
ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG 
CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG 
AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA 
TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG 
AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA 
GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG 
CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC 
ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT 
GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG 
ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA 
GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA 
GTGAACAGTG TGTCGACTCC TCCTCAATCT CCAAGCTCCT CCTCCTCTCC TCCGCCTCCA 
CCCTCCCCTC CACAGCTGAC GGACGGGTCA CACTTCATGT GTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298285.1
CDS	42..3929
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISVNSVSTPPQSPSSSSSPPPPPSPPQLTDGSHFMCV

Target ORF information:

RefSeq Version	XM_009300010.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300010.3

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG 
GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA 
ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG 
CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG 
GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC 
GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA 
CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG 
CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG 
GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT 
CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG 
CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA 
GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC 
AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG 
ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG 
CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG 
AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA 
TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG 
AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA 
GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG 
CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC 
ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT 
GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG 
ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA 
GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA 
GTGAACAGTG TGTCGACTCC TCCTCAATCT CCAAGCTCCT CCTCCTCTCC TCCGCCTCCA 
CCCTCCCCTC CACAGCTGAC GGACGGGTCA CACTTCATGT GTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300011.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298286.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X15, mRNA.

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CloneID	ODa45119
Clone ID Related Accession (Same CDS sequence)	XM_009300011.2 , XM_009300011.3
Accession Version	XM_009300011.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC 
AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA 
GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG 
GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG 
AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG 
AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT 
GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG 
GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG 
ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA 
AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG 
CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG 
ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC 
AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG 
AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT 
GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG 
ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC 
TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT 
AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG 
GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG 
GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG 
GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC 
GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG 
GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA 
GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG 
ATCTCAGTGA ACAGTGTGTC GACTCCTCCT CAATCTCCAA GCTCCTCCTC CTCTCCTCCG 
CCTCCACCCT CCCCTCCACA GCTGACGGAC GGGTCACACT TCATGTGTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298286.1
CDS	43..3756
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISVNSVSTPPQSPSSSSSPPPPPSPPQLTDGSHFMCV

Target ORF information:

RefSeq Version	XM_009300011.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300011.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC 
AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA 
GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG 
GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG 
AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG 
AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT 
GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG 
GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG 
ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA 
AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG 
CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG 
ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC 
AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG 
AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT 
GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG 
ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC 
TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT 
AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG 
GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG 
GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG 
GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC 
GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG 
GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA 
GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG 
ATCTCAGTGA ACAGTGTGTC GACTCCTCCT CAATCTCCAA GCTCCTCCTC CTCTCCTCCG 
CCTCCACCCT CCCCTCCACA GCTGACGGAC GGGTCACACT TCATGTGTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300011.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298286.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X23, mRNA.

Price & Availability↑

CloneID	ODa45119
Clone ID Related Accession (Same CDS sequence)	XM_009300011.2 , XM_009300011.3
Accession Version	XM_009300011.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300011.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC 
AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA 
GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG 
GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG 
AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG 
AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT 
GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG 
GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG 
ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA 
AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG 
CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG 
ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC 
AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG 
AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT 
GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG 
ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC 
TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT 
AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG 
GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG 
GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG 
GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC 
GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG 
GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA 
GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG 
ATCTCAGTGA ACAGTGTGTC GACTCCTCCT CAATCTCCAA GCTCCTCCTC CTCTCCTCCG 
CCTCCACCCT CCCCTCCACA GCTGACGGAC GGGTCACACT TCATGTGTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298286.1
CDS	42..3755
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISVNSVSTPPQSPSSSSSPPPPPSPPQLTDGSHFMCV

Target ORF information:

RefSeq Version	XM_009300011.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300011.3

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC 
AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA 
GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG 
GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG 
AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG 
AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT 
GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG 
GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG 
ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA 
AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG 
CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG 
ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC 
AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG 
AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT 
GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG 
ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC 
TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT 
AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG 
GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG 
GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG 
GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC 
GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG 
GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA 
GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG 
ATCTCAGTGA ACAGTGTGTC GACTCCTCCT CAATCTCCAA GCTCCTCCTC CTCTCCTCCG 
CCTCCACCCT CCCCTCCACA GCTGACGGAC GGGTCACACT TCATGTGTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekha5 ( XM_009300012.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298287.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ODa45120
Clone ID Related Accession (Same CDS sequence)	XM_009300012.2
Accession Version	XM_009300012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pleckstrin homology domain-containing family A member 5 isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAAATGA GTCCTGAAGA GTACAGAGAA 
AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT 
GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG 
CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC 
CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC 
ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT 
CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC 
GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG 
AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC 
AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC 
TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG 
CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC 
CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT 
GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG 
CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG 
GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC 
TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC 
GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG 
CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT 
GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA 
CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT 
GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT 
AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC 
TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG 
GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG 
ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG 
AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA 
CTGATCTCAG TGAACAGTGT GTCGACTCCT CCTCAATCTC CAAGCTCCTC CTCCTCTCCT 
CCGCCTCCAC CCTCCCCTCC ACAGCTGACG GACGGGTCAC ACTTCATGTG TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298287.1
CDS	44..4180
Translation	MAADLNPDWLSCLPSSWSYGVTRDGRVFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFINHNERKVTCKHPVSGVPSQDNCIFVVNEQPATKAPEAEKKERPTSTMSEASNYTGGSDYTTHPSSPTTRPSRSSKKVHNFGKRSNSIKRNPNAPVIKNGWLHKQDSTGMKMWKKRWFVLSDMCLFYYRDEKEEGILGSILLPSFHISMLSVDDHITRKYAFKATHPNMRTYYFSTDTAKDMESWMKVMSDAAMAHSEPIRRLEKVKLDSRVPQEMNNMLNHRVLTRPEIQNNERNRETLRQEDKKSKPSEKPQKDKDRVSMQKDGERFSENYTHRQKDGERYMLQKDLEKFVLQKDGEKYAVQKEGDKYLLQKDGHKYTVHKDGDKYTLQRELEMYNPPKDAEKIPQKDARFSLVQKELEKPVPPPRSEEKYGFQKEGTIERPLTKINSIKLQPAQAAAIAAAVSSSRMMQKQVNGSGERAGDRSPGDLSTVRTSVPPPTQPAPEPERSLSRTSSMQQLEQWVRTHRTRAPDDDTRSITSYQTLPRNMPSHRAQIVPRYPEGYRTLPRNMLRPDSICSVAGSVYDRALQPSTQEKRRSIRDDTMWQLYEWQQRQAHSRLGYGTLPSPKTMGQIAESIPTSPSHGSLAGYHTFSPNRPLNPDSRSELSSPVFRGDVTIERRHRPHLSKYSYPAERRAAPPAQNITAQSLQGKTPEELTLLLIKLRRQQAELNSLREHTLAQLMQLNLDATNPKSEILSHHLQRNLMYLDSQMKENEPIIFMIHTMIENSAPRPQLYQQMSPEEYRENTYMYRPEELDVDAKLSRLCEQDKVVRTQEEKLQQLHREKHTLETALLSASQEIEMSSENPAAVQSVVQQRDVLQSGLLSTCRELSRVSTELERSWREYDRLEADVTLAKTNLLEQLEALGSPQTEPPSQKHVQIQKELWRIQDVMEALSKNKPKRNPEPSFPGANPLSTLHKSEDSVDSSCVPPRPPLPYSYEGGDRPPPLPNPGQPHTTRTTPHRPEDRKTAQRNGTHTGPDYRLYKSEPELTTVAEVDESNGEDKSEQTAEKEPPGVTKGIVYPVGVVSPRTKSPMPESSTIASYVTLRKTKKPDPRTCQPQDRPRSAVEQMSSGAEVGRTRMSVEEQMERIRRHQQGALRERRREDGSLSRSLSFTKDSTYYTLQARKKSPVISPDEQDERRDMTPVERDTKHTKHPDAEENCRDTKEQLPAHTHLEIKDKASVTRSVSMKESLLKSVSPKPVLKQDESECKLEYDRKLATQNSLQTAVMVRVGTEEDEEDEEEEQKDSVQQQEDSYELSNSKHSTLISVNSVSTPPQSPSSSSSPPPPPSPPQLTDGSHFMCV

Target ORF information:

RefSeq Version	XM_009300012.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300012.2

ORF Insert Sequence:

ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG 
CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC 
GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA 
AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG 
GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC 
ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC 
CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC 
TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC 
AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC 
TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC 
ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT 
CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG 
AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA 
AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA 
CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG 
AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA 
GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG 
AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA 
GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC 
AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC 
CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA 
CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT 
AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA 
TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT 
CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA 
CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA 
CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA 
AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT 
CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC 
CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG 
CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC 
AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT 
CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG 
CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG 
TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA 
CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG 
CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC 
CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG 
TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT 
GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAAATGA GTCCTGAAGA GTACAGAGAA 
AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT 
GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG 
CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC 
CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC 
ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT 
CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC 
GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG 
AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC 
AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC 
TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG 
CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC 
CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT 
GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG 
CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG 
GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC 
TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC 
GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG 
CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT 
GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA 
CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT 
GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT 
AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC 
TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG 
GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG 
ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG 
AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA 
CTGATCTCAG TGAACAGTGT GTCGACTCCT CCTCAATCTC CAAGCTCCTC CTCCTCTCCT 
CCGCCTCCAC CCTCCCCTCC ACAGCTGACG GACGGGTCAC ACTTCATGTG TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.
Scientific reports7(1)10381(2017 Sep)
Ma Z,Zhu P,Pang M,Guo L,Chang N,Zheng J,Zhu X,Gao C,Huang H,Cui Z,Xiong JW,Peng J,Chen J

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A novel neuropeptide in suppressing luteinizing hormone release in goldfish, Carassius auratus.
Molecular and cellular endocrinology374(1-2)65-72(2013 Jul)
Liu Y,Li S,Qi X,Zhou W,Liu X,Lin H,Zhang Y,Cheng CH

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plekha5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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