OPN5 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	OPN5
Entrez Gene ID	564181
Full Name	opsin 5
Synonyms	Opn5m,si:ch211-105n9.2
General protein information	Preferred Names opsin 5 Names opsin-5 neuropsin teleost neuropsin
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009294767.2	XP_009293042.1	opsin-5 isoform X2
NM_001200046.1	NP_001186975.1	opsin-5
XM_009294766.3	XP_009293041.1	opsin-5 isoform X1
XM_009294766.2	XP_009293041.1	opsin-5 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Opn5	NP_861437.1
Homo sapiens (human)	OPN5	NP_859528.1
	OPN5	XP_001104044.1
Bos taurus (cattle)	OPN5	NP_001193009.1
Canis lupus familiaris (dog)	OPN5	XP_003639476.1
Mus musculus (house mouse)	Opn5	NP_861418.2
Gallus gallus (chicken)	OPN5	NP_001124215.1
Danio rerio (zebrafish)	opn5	NP_001186975.1
Xenopus tropicalis (tropical clawed frog)	opn5	XP_002936036.1
Pan troglodytes (chimpanzee)	OPN5	XP_001146167.1

Gene Ontology
Bibliography

Related articles in PubMed

The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons.
Braasch I, Gehrke AR, Smith JJ, Kawasaki K, Manousaki T, Pasquier J, Amores A, Desvignes T, Batzel P, Catchen J, Berlin AM, Campbell MS, Barrell D, Martin KJ, Mulley JF, Ravi V, Lee AP, Nakamura T, Chalopin D, Fan S, Wcisel D, Ca?estro C, Sydes J, Beaudry FE, Sun Y, Hertel J, Beam MJ, Fasold M, Ishiyama M, Johnson J, Kehr S, Lara M, Letaw JH, Litman GW, Litman RT, Mikami M, Ota T, Saha NR, Williams L, Stadler PF, Wang H, Taylor JS, Fontenot Q, Ferrara A, Searle SM, Aken B, Yandell M, Schneider I, Yoder JA, Volff JN, Meyer A, Amemiya CT, Venkatesh B, Holland PW, Guiguen Y, Bobe J, Shubin NH, Di Palma F, Alf?ldi J, Lindblad-Toh K, Postlethwait JH
Nature genetics48(4)427-37(2016 Apr)

Two UV-Sensitive Photoreceptor Proteins, Opn5m and Opn5m2 in Ray-Finned Fish with Distinct Molecular Properties and Broad Distribution in the Retina and Brain.
Sato K, Yamashita T, Haruki Y, Ohuchi H, Kinoshita M, Shichida Y
PloS one11(5)e0155339(2016)

An extended family of novel vertebrate photopigments is widely expressed and displays a diversity of function.
Davies WI, Tamai TK, Zheng L, Fu JK, Rihel J, Foster RG, Whitmore D, Hankins MW
Genome research25(11)1666-79(2015 Nov)

Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation.
Yamashita T, Ono K, Ohuchi H, Yumoto A, Gotoh H, Tomonari S, Sakai K, Fujita H, Imamoto Y, Noji S, Nakamura K, Shichida Y
The Journal of biological chemistry289(7)3991-4000(2014 Feb)

Light-induced body color change in developing zebrafish.
Shiraki T, Kojima D, Fukada Y
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology9(11)1498-504(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel opsin isoform, Opn5m2, exhibits UV light-sensitivity when binding to 11-cis-retinal. It displays distinct molecular properties and broad distribution in the retina and brain.
Title: Two UV-Sensitive Photoreceptor Proteins, Opn5m and Opn5m2 in Ray-Finned Fish with Distinct Molecular Properties and Broad Distribution in the Retina and Brain.

Opn5m proteins in zebrafish, Xenopus tropicalis, mouse, and human are also UV-sensitive pigments.
Title: Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation.

The following OPN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OPN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01484	NM_001200046.1 Latest version!	Danio rerio opsin 5 (opn5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa25022	XM_009294766.3 Latest version!	Danio rerio opsin 5 (opn5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa44256	XM_009294767.2 Latest version!	Danio rerio opsin 5 (opn5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
ODa25022	XM_009294766.2	Danio rerio opsin 5 (opn5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OPN5 ( NM_001200046.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001186975.1 Danio rerio opsin 5 (opn5), mRNA.

Price & Availability↑

CloneID	ODa01484
Clone ID Related Accession (Same CDS sequence)	NM_001200046.1
Accession Version	NM_001200046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-09
Organism	Danio rerio(zebrafish)
Product	opsin-5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY493740.1. On Jan 16, 2011 this sequence version replaced XM_687530.1. ##Evidence-Data-START## Transcript exon combination :: AY493740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705, SAMN06350654 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAATG AGACCTCCAT CTCCTCGGGA TACATCCCAC ATTACCTCCT TCGAGGGGAT 
CCCTTTGCCT CCAAGTTGTC CAAGGAGGCA GACATAGTGG CAGCGTTCTA CATCCTTGTC 
ATCGGTATCT TGTCAGCCAC TGGAAATGGC TATGTCATGT ACATGACCTT CAAGCGAAAA 
ACAAAGTTAA AGCCACCAGA GATCATGACA CTCAATCTAG CTATATTCGA CTTTGGCATA 
TCAGTGAGTG GAAAACCGTT CTTCATAGTG TCTAGTTTTT CTCACCGCTG GCTGTTCGGC 
TGGCAGGGCT GCCGGTACTA TGGCTGGGCC GGATTCTTCT TTGGATGTGG AAGTCTGATT 
ACTATGACTG TTGTCAGCTT TGACCGCTAC TTGAAAATTT GTCATTTAAG ATATGGCACT 
TGGTTGAAGC GTCATCATGC TTTCCTCTCG GTGGTTTTCA TTTGGGCTTA TGCTGCATTC 
TGGGCCACCA TGCCAGTGGT GGGCTGGGGT AACTACGCAC CTGAGCCGTT TGGCACCTCT 
TGCACACTGG ACTGGTGGTT GACACAGGCC TCAGTGTCTG GCCAGAGCTT TGTGATGTGC 
ATGCTGTTCT TCTGTCTCAT CTTCCCCACC GTCATCATCG TCTTCTCCTA CGTCATGATC 
ATCTTCAAGG TGAAATCCTC AGCCAAAGAA GTCTCTCACT TCGACACACG AAATAAGAAC 
AACCACAGTC TAGAGATGAA GCTGACTAAG GTGGCGATGT TGATCTGTGC AGGGTTTTTG 
ATAGCCTGGA TCCCGTATGC TGTGGTGTCA GTGATGTCTG CTTTTGGAGA GCCTGATTCA 
GTGCCTATTC CTGTCTCTGT TGTGCCAACT TTGCTGGCCA AATCCTCTGC CATGTACAAT 
CCGATCATAT ATCAGGTCAT TGACTGTAAA AAGAAGTGTG TAAAATCATG CTGTTTTCAA 
GCTTGGAGAA AAAAGAAACC ATCCAAGACC TCAAGGTTCT ACACCATCTC TGGCTCCATC 
AAACAGAGAC CAGCCGATGA AGCTTCTATT GAGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186975.1
CDS	1..1059
Translation	MENETSISSGYIPHYLLRGDPFASKLSKEADIVAAFYILVIGILSATGNGYVMYMTFKRKTKLKPPEIMTLNLAIFDFGISVSGKPFFIVSSFSHRWLFGWQGCRYYGWAGFFFGCGSLITMTVVSFDRYLKICHLRYGTWLKRHHAFLSVVFIWAYAAFWATMPVVGWGNYAPEPFGTSCTLDWWLTQASVSGQSFVMCMLFFCLIFPTVIIVFSYVMIIFKVKSSAKEVSHFDTRNKNNHSLEMKLTKVAMLICAGFLIAWIPYAVVSVMSAFGEPDSVPIPVSVVPTLLAKSSAMYNPIIYQVIDCKKKCVKSCCFQAWRKKKPSKTSRFYTISGSIKQRPADEASIEI

Target ORF information:

RefSeq Version	NM_001200046.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio opsin 5 (opn5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200046.1

ORF Insert Sequence:

ATGGAGAATG AGACCTCCAT CTCCTCGGGA TACATCCCAC ATTACCTCCT TCGAGGGGAT 
CCCTTTGCCT CCAAGTTGTC CAAGGAGGCA GACATAGTGG CAGCGTTCTA CATCCTTGTC 
ATCGGTATCT TGTCAGCCAC TGGAAATGGC TATGTCATGT ACATGACCTT CAAGCGAAAA 
ACAAAGTTAA AGCCACCAGA GATCATGACA CTCAATCTAG CTATATTCGA CTTTGGCATA 
TCAGTGAGTG GAAAACCGTT CTTCATAGTG TCTAGTTTTT CTCACCGCTG GCTGTTCGGC 
TGGCAGGGCT GCCGGTACTA TGGCTGGGCC GGATTCTTCT TTGGATGTGG AAGTCTGATT 
ACTATGACTG TTGTCAGCTT TGACCGCTAC TTGAAAATTT GTCATTTAAG ATATGGCACT 
TGGTTGAAGC GTCATCATGC TTTCCTCTCG GTGGTTTTCA TTTGGGCTTA TGCTGCATTC 
TGGGCCACCA TGCCAGTGGT GGGCTGGGGT AACTACGCAC CTGAGCCGTT TGGCACCTCT 
TGCACACTGG ACTGGTGGTT GACACAGGCC TCAGTGTCTG GCCAGAGCTT TGTGATGTGC 
ATGCTGTTCT TCTGTCTCAT CTTCCCCACC GTCATCATCG TCTTCTCCTA CGTCATGATC 
ATCTTCAAGG TGAAATCCTC AGCCAAAGAA GTCTCTCACT TCGACACACG AAATAAGAAC 
AACCACAGTC TAGAGATGAA GCTGACTAAG GTGGCGATGT TGATCTGTGC AGGGTTTTTG 
ATAGCCTGGA TCCCGTATGC TGTGGTGTCA GTGATGTCTG CTTTTGGAGA GCCTGATTCA 
GTGCCTATTC CTGTCTCTGT TGTGCCAACT TTGCTGGCCA AATCCTCTGC CATGTACAAT 
CCGATCATAT ATCAGGTCAT TGACTGTAAA AAGAAGTGTG TAAAATCATG CTGTTTTCAA 
GCTTGGAGAA AAAAGAAACC ATCCAAGACC TCAAGGTTCT ACACCATCTC TGGCTCCATC 
AAACAGAGAC CAGCCGATGA AGCTTCTATT GAGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OPN5 ( XM_009294766.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009293041.1 Danio rerio opsin 5 (opn5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25022
Clone ID Related Accession (Same CDS sequence)	XM_009294766.3 , XM_009294766.2
Accession Version	XM_009294766.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	opsin-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATG AGACCTCCAT CTCCTCGGGA TACATCCCAC ATTACCTCCT TCGAGGGGAT 
CCCTTTGCCT CCAAGTTGTC CAAGGAGGCA GACATAGTGG CAGCATTCTA CATCCTTGTC 
ATCGGTATCT TGTCAGCCAC TGGAAATGGC TATGTCATGT ACATGACCTT CAAGCGAAAA 
ACAAAGTTAA AGCCACCAGA GATCATGACA CTCAATCTAG CTATATTCGA CTTTGGCATA 
TCAGTGAGTG GAAAACCGTT CTTCATAGTG TCTAGTTTTT CTCACCGCTG GCTGTTCGGC 
TGGCAGGGCT GCCGGTACTA TGGCTGGGCC GGATTCTTCT TTGGATGTGG AAGTCTGATT 
ACTATGACTG TTGTCAGCTT TGACCGCTAC TTGAAAATTT GTCATTTAAG ATATGGCACT 
TGGTTGAAGC GTCATCATGC TTTCCTCTCG GTGGTTTTCA TTTGGGCTTA TGCTGCATTC 
TGGGCCACCA TGCCAGTGGT GGGCTGGGGT AACTACGCAC CTGAGCCGTT TGGCACCTCT 
TGCACACTGG ACTGGTGGTT GACACAGGCC TCAGTGTCTG GCCAGAGCTT TGTGATGTGC 
ATGCTGTTCT TCTGTCTCAT CTTCCCCACC GTCATCATCG TCTTCTCCTA CGTCATGATC 
ATCTTCAAGG TGAAATCCTC AGCCAAAGAA GTCTCTCACT TCGACACACG AAATAAGAAC 
AACCACAGTC TAGAGATGAA GCTGACTAAG GTGGCGATGT TGATCTGTGC AGGGTTTTTG 
ATAGCCTGGA TCCCGTATGC TGTGGTGTCA GTGATGTCTG CTTTTGGAGA GCCTGATTCA 
GTGCCTATTC CTGTCTCTGT TGTGCCAACT TTGCTGGCCA AATCCTCTGC CATGTACAAT 
CCGATCATAT ATCAGGTCAT TGACTGTAAA AAGAAGTGTG TAAAATCATG CTGTTTTCAA 
GCTTGGAGAA AAAAGAAACC ATCCAAGACC TCAAGGTTCT ACACCATCTC TGGCTCCATC 
AAACAGAGAC CAGGCGATGA AGCTTCTATT GAGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293041.1
CDS	459..1517
Translation	MENETSISSGYIPHYLLRGDPFASKLSKEADIVAAFYILVIGILSATGNGYVMYMTFKRKTKLKPPEIMTLNLAIFDFGISVSGKPFFIVSSFSHRWLFGWQGCRYYGWAGFFFGCGSLITMTVVSFDRYLKICHLRYGTWLKRHHAFLSVVFIWAYAAFWATMPVVGWGNYAPEPFGTSCTLDWWLTQASVSGQSFVMCMLFFCLIFPTVIIVFSYVMIIFKVKSSAKEVSHFDTRNKNNHSLEMKLTKVAMLICAGFLIAWIPYAVVSVMSAFGEPDSVPIPVSVVPTLLAKSSAMYNPIIYQVIDCKKKCVKSCCFQAWRKKKPSKTSRFYTISGSIKQRPGDEASIEI

Target ORF information:

RefSeq Version	XM_009294766.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio opsin 5 (opn5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294766.3

ORF Insert Sequence:

ATGGAGAATG AGACCTCCAT CTCCTCGGGA TACATCCCAC ATTACCTCCT TCGAGGGGAT 
CCCTTTGCCT CCAAGTTGTC CAAGGAGGCA GACATAGTGG CAGCATTCTA CATCCTTGTC 
ATCGGTATCT TGTCAGCCAC TGGAAATGGC TATGTCATGT ACATGACCTT CAAGCGAAAA 
ACAAAGTTAA AGCCACCAGA GATCATGACA CTCAATCTAG CTATATTCGA CTTTGGCATA 
TCAGTGAGTG GAAAACCGTT CTTCATAGTG TCTAGTTTTT CTCACCGCTG GCTGTTCGGC 
TGGCAGGGCT GCCGGTACTA TGGCTGGGCC GGATTCTTCT TTGGATGTGG AAGTCTGATT 
ACTATGACTG TTGTCAGCTT TGACCGCTAC TTGAAAATTT GTCATTTAAG ATATGGCACT 
TGGTTGAAGC GTCATCATGC TTTCCTCTCG GTGGTTTTCA TTTGGGCTTA TGCTGCATTC 
TGGGCCACCA TGCCAGTGGT GGGCTGGGGT AACTACGCAC CTGAGCCGTT TGGCACCTCT 
TGCACACTGG ACTGGTGGTT GACACAGGCC TCAGTGTCTG GCCAGAGCTT TGTGATGTGC 
ATGCTGTTCT TCTGTCTCAT CTTCCCCACC GTCATCATCG TCTTCTCCTA CGTCATGATC 
ATCTTCAAGG TGAAATCCTC AGCCAAAGAA GTCTCTCACT TCGACACACG AAATAAGAAC 
AACCACAGTC TAGAGATGAA GCTGACTAAG GTGGCGATGT TGATCTGTGC AGGGTTTTTG 
ATAGCCTGGA TCCCGTATGC TGTGGTGTCA GTGATGTCTG CTTTTGGAGA GCCTGATTCA 
GTGCCTATTC CTGTCTCTGT TGTGCCAACT TTGCTGGCCA AATCCTCTGC CATGTACAAT 
CCGATCATAT ATCAGGTCAT TGACTGTAAA AAGAAGTGTG TAAAATCATG CTGTTTTCAA 
GCTTGGAGAA AAAAGAAACC ATCCAAGACC TCAAGGTTCT ACACCATCTC TGGCTCCATC 
AAACAGAGAC CAGGCGATGA AGCTTCTATT GAGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OPN5 ( XM_009294766.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009293041.1 Danio rerio opsin 5 (opn5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25022
Clone ID Related Accession (Same CDS sequence)	XM_009294766.3 , XM_009294766.2
Accession Version	XM_009294766.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	opsin-5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATG AGACCTCCAT CTCCTCGGGA TACATCCCAC ATTACCTCCT TCGAGGGGAT 
CCCTTTGCCT CCAAGTTGTC CAAGGAGGCA GACATAGTGG CAGCATTCTA CATCCTTGTC 
ATCGGTATCT TGTCAGCCAC TGGAAATGGC TATGTCATGT ACATGACCTT CAAGCGAAAA 
ACAAAGTTAA AGCCACCAGA GATCATGACA CTCAATCTAG CTATATTCGA CTTTGGCATA 
TCAGTGAGTG GAAAACCGTT CTTCATAGTG TCTAGTTTTT CTCACCGCTG GCTGTTCGGC 
TGGCAGGGCT GCCGGTACTA TGGCTGGGCC GGATTCTTCT TTGGATGTGG AAGTCTGATT 
ACTATGACTG TTGTCAGCTT TGACCGCTAC TTGAAAATTT GTCATTTAAG ATATGGCACT 
TGGTTGAAGC GTCATCATGC TTTCCTCTCG GTGGTTTTCA TTTGGGCTTA TGCTGCATTC 
TGGGCCACCA TGCCAGTGGT GGGCTGGGGT AACTACGCAC CTGAGCCGTT TGGCACCTCT 
TGCACACTGG ACTGGTGGTT GACACAGGCC TCAGTGTCTG GCCAGAGCTT TGTGATGTGC 
ATGCTGTTCT TCTGTCTCAT CTTCCCCACC GTCATCATCG TCTTCTCCTA CGTCATGATC 
ATCTTCAAGG TGAAATCCTC AGCCAAAGAA GTCTCTCACT TCGACACACG AAATAAGAAC 
AACCACAGTC TAGAGATGAA GCTGACTAAG GTGGCGATGT TGATCTGTGC AGGGTTTTTG 
ATAGCCTGGA TCCCGTATGC TGTGGTGTCA GTGATGTCTG CTTTTGGAGA GCCTGATTCA 
GTGCCTATTC CTGTCTCTGT TGTGCCAACT TTGCTGGCCA AATCCTCTGC CATGTACAAT 
CCGATCATAT ATCAGGTCAT TGACTGTAAA AAGAAGTGTG TAAAATCATG CTGTTTTCAA 
GCTTGGAGAA AAAAGAAACC ATCCAAGACC TCAAGGTTCT ACACCATCTC TGGCTCCATC 
AAACAGAGAC CAGGCGATGA AGCTTCTATT GAGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293041.1
CDS	401..1459
Translation	MENETSISSGYIPHYLLRGDPFASKLSKEADIVAAFYILVIGILSATGNGYVMYMTFKRKTKLKPPEIMTLNLAIFDFGISVSGKPFFIVSSFSHRWLFGWQGCRYYGWAGFFFGCGSLITMTVVSFDRYLKICHLRYGTWLKRHHAFLSVVFIWAYAAFWATMPVVGWGNYAPEPFGTSCTLDWWLTQASVSGQSFVMCMLFFCLIFPTVIIVFSYVMIIFKVKSSAKEVSHFDTRNKNNHSLEMKLTKVAMLICAGFLIAWIPYAVVSVMSAFGEPDSVPIPVSVVPTLLAKSSAMYNPIIYQVIDCKKKCVKSCCFQAWRKKKPSKTSRFYTISGSIKQRPGDEASIEI

Target ORF information:

RefSeq Version	XM_009294766.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio opsin 5 (opn5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294766.2

ORF Insert Sequence:

ATGGAGAATG AGACCTCCAT CTCCTCGGGA TACATCCCAC ATTACCTCCT TCGAGGGGAT 
CCCTTTGCCT CCAAGTTGTC CAAGGAGGCA GACATAGTGG CAGCATTCTA CATCCTTGTC 
ATCGGTATCT TGTCAGCCAC TGGAAATGGC TATGTCATGT ACATGACCTT CAAGCGAAAA 
ACAAAGTTAA AGCCACCAGA GATCATGACA CTCAATCTAG CTATATTCGA CTTTGGCATA 
TCAGTGAGTG GAAAACCGTT CTTCATAGTG TCTAGTTTTT CTCACCGCTG GCTGTTCGGC 
TGGCAGGGCT GCCGGTACTA TGGCTGGGCC GGATTCTTCT TTGGATGTGG AAGTCTGATT 
ACTATGACTG TTGTCAGCTT TGACCGCTAC TTGAAAATTT GTCATTTAAG ATATGGCACT 
TGGTTGAAGC GTCATCATGC TTTCCTCTCG GTGGTTTTCA TTTGGGCTTA TGCTGCATTC 
TGGGCCACCA TGCCAGTGGT GGGCTGGGGT AACTACGCAC CTGAGCCGTT TGGCACCTCT 
TGCACACTGG ACTGGTGGTT GACACAGGCC TCAGTGTCTG GCCAGAGCTT TGTGATGTGC 
ATGCTGTTCT TCTGTCTCAT CTTCCCCACC GTCATCATCG TCTTCTCCTA CGTCATGATC 
ATCTTCAAGG TGAAATCCTC AGCCAAAGAA GTCTCTCACT TCGACACACG AAATAAGAAC 
AACCACAGTC TAGAGATGAA GCTGACTAAG GTGGCGATGT TGATCTGTGC AGGGTTTTTG 
ATAGCCTGGA TCCCGTATGC TGTGGTGTCA GTGATGTCTG CTTTTGGAGA GCCTGATTCA 
GTGCCTATTC CTGTCTCTGT TGTGCCAACT TTGCTGGCCA AATCCTCTGC CATGTACAAT 
CCGATCATAT ATCAGGTCAT TGACTGTAAA AAGAAGTGTG TAAAATCATG CTGTTTTCAA 
GCTTGGAGAA AAAAGAAACC ATCCAAGACC TCAAGGTTCT ACACCATCTC TGGCTCCATC 
AAACAGAGAC CAGGCGATGA AGCTTCTATT GAGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OPN5 ( XM_009294767.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009293042.1 Danio rerio opsin 5 (opn5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44256
Clone ID Related Accession (Same CDS sequence)	XM_009294767.2
Accession Version	XM_009294767.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	opsin-5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATG AGACCTCCAT CTCCTCGGGA TACATCCCAC ATTACCTCCT TCGAGGGGAT 
CCCTTTGCCT CCAAGTTGTC CAAGGAGGCA GACATAGTGG CAGCATTCTA CATCCTTGTC 
ATCGGTATCT TGTCAGCCAC TGGAAATGGC TATGTCATGT ACATGACCTT CAAGCGAAAA 
ACAAAGTTAA AGCCACCAGA GATCATGACA CTCAATCTAG CTATATTCGA CTTTGGCATA 
TCAGGCACTT GGTTGAAGCG TCATCATGCT TTCCTCTCGG TGGTTTTCAT TTGGGCTTAT 
GCTGCATTCT GGGCCACCAT GCCAGTGGTG GGCTGGGGTA ACTACGCACC TGAGCCGTTT 
GGCACCTCTT GCACACTGGA CTGGTGGTTG ACACAGGCCT CAGTGTCTGG CCAGAGCTTT 
GTGATGTGCA TGCTGTTCTT CTGTCTCATC TTCCCCACCG TCATCATCGT CTTCTCCTAC 
GTCATGATCA TCTTCAAGGT GAAATCCTCA GCCAAAGAAG TCTCTCACTT CGACACACGA 
AATAAGAACA ACCACAGTCT AGAGATGAAG CTGACTAAGG TGGCGATGTT GATCTGTGCA 
GGGTTTTTGA TAGCCTGGAT CCCGTATGCT GTGGTGTCAG TGATGTCTGC TTTTGGAGAG 
CCTGATTCAG TGCCTATTCC TGTCTCTGTT GTGCCAACTT TGCTGGCCAA ATCCTCTGCC 
ATGTACAATC CGATCATATA TCAGGTCATT GACTGTAAAA AGAAGTGTGT AAAATCATGC 
TGTTTTCAAG CTTGGAGAAA AAAGAAACCA TCCAAGACCT CAAGGTTCTA CACCATCTCT 
GGCTCCATCA AACAGAGACC AGGCGATGAA GCTTCTATTG AGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293042.1
CDS	401..1288
Translation	MENETSISSGYIPHYLLRGDPFASKLSKEADIVAAFYILVIGILSATGNGYVMYMTFKRKTKLKPPEIMTLNLAIFDFGISGTWLKRHHAFLSVVFIWAYAAFWATMPVVGWGNYAPEPFGTSCTLDWWLTQASVSGQSFVMCMLFFCLIFPTVIIVFSYVMIIFKVKSSAKEVSHFDTRNKNNHSLEMKLTKVAMLICAGFLIAWIPYAVVSVMSAFGEPDSVPIPVSVVPTLLAKSSAMYNPIIYQVIDCKKKCVKSCCFQAWRKKKPSKTSRFYTISGSIKQRPGDEASIEI

Target ORF information:

RefSeq Version	XM_009294767.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio opsin 5 (opn5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294767.2

ORF Insert Sequence:

ATGGAGAATG AGACCTCCAT CTCCTCGGGA TACATCCCAC ATTACCTCCT TCGAGGGGAT 
CCCTTTGCCT CCAAGTTGTC CAAGGAGGCA GACATAGTGG CAGCATTCTA CATCCTTGTC 
ATCGGTATCT TGTCAGCCAC TGGAAATGGC TATGTCATGT ACATGACCTT CAAGCGAAAA 
ACAAAGTTAA AGCCACCAGA GATCATGACA CTCAATCTAG CTATATTCGA CTTTGGCATA 
TCAGGCACTT GGTTGAAGCG TCATCATGCT TTCCTCTCGG TGGTTTTCAT TTGGGCTTAT 
GCTGCATTCT GGGCCACCAT GCCAGTGGTG GGCTGGGGTA ACTACGCACC TGAGCCGTTT 
GGCACCTCTT GCACACTGGA CTGGTGGTTG ACACAGGCCT CAGTGTCTGG CCAGAGCTTT 
GTGATGTGCA TGCTGTTCTT CTGTCTCATC TTCCCCACCG TCATCATCGT CTTCTCCTAC 
GTCATGATCA TCTTCAAGGT GAAATCCTCA GCCAAAGAAG TCTCTCACTT CGACACACGA 
AATAAGAACA ACCACAGTCT AGAGATGAAG CTGACTAAGG TGGCGATGTT GATCTGTGCA 
GGGTTTTTGA TAGCCTGGAT CCCGTATGCT GTGGTGTCAG TGATGTCTGC TTTTGGAGAG 
CCTGATTCAG TGCCTATTCC TGTCTCTGTT GTGCCAACTT TGCTGGCCAA ATCCTCTGCC 
ATGTACAATC CGATCATATA TCAGGTCATT GACTGTAAAA AGAAGTGTGT AAAATCATGC 
TGTTTTCAAG CTTGGAGAAA AAAGAAACCA TCCAAGACCT CAAGGTTCTA CACCATCTCT 
GGCTCCATCA AACAGAGACC AGGCGATGAA GCTTCTATTG AGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons.
Nature genetics48(4)427-37(2016 Apr)
Braasch I,Gehrke AR,Smith JJ,Kawasaki K,Manousaki T,Pasquier J,Amores A,Desvignes T,Batzel P,Catchen J,Berlin AM,Campbell MS,Barrell D,Martin KJ,Mulley JF,Ravi V,Lee AP,Nakamura T,Chalopin D,Fan S,Wcisel D,Ca?estro C,Sydes J,Beaudry FE,Sun Y,Hertel J,Beam MJ,Fasold M,Ishiyama M,Johnson J,Kehr S,Lara M,Letaw JH,Litman GW,Litman RT,Mikami M,Ota T,Saha NR,Williams L,Stadler PF,Wang H,Taylor JS,Fontenot Q,Ferrara A,Searle SM,Aken B,Yandell M,Schneider I,Yoder JA,Volff JN,Meyer A,Amemiya CT,Venkatesh B,Holland PW,Guiguen Y,Bobe J,Shubin NH,Di Palma F,Alf?ldi J,Lindblad-Toh K,Postlethwait JH

Two UV-Sensitive Photoreceptor Proteins, Opn5m and Opn5m2 in Ray-Finned Fish with Distinct Molecular Properties and Broad Distribution in the Retina and Brain.
PloS one11(5)e0155339(2016)
Sato K,Yamashita T,Haruki Y,Ohuchi H,Kinoshita M,Shichida Y

An extended family of novel vertebrate photopigments is widely expressed and displays a diversity of function.
Genome research25(11)1666-79(2015 Nov)
Davies WI,Tamai TK,Zheng L,Fu JK,Rihel J,Foster RG,Whitmore D,Hankins MW

Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation.
The Journal of biological chemistry289(7)3991-4000(2014 Feb)
Yamashita T,Ono K,Ohuchi H,Yumoto A,Gotoh H,Tomonari S,Sakai K,Fujita H,Imamoto Y,Noji S,Nakamura K,Shichida Y

Light-induced body color change in developing zebrafish.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology9(11)1498-504(2010 Nov)
Shiraki T,Kojima D,Fukada Y

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OPN5 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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