sema5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema5a
Entrez Gene ID	561295
Full Name	sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms	wu:fa95e03,wu:fi41a04
General protein information	Preferred Names sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A Names semaphorin-5A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353840.1	XP_017209329.1	semaphorin-5A isoform X1
XM_021470194.1	XP_021325869.1	semaphorin-5A isoform X2
XM_005162619.3	XP_005162676.1	semaphorin-5A isoform X1
NM_001271670.1	NP_001258599.1	semaphorin-5A
XM_005162619.4	XP_005162676.1	semaphorin-5A isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SEMA5A	XP_005639731.1
Rattus norvegicus (Norway rat)	Sema5a	NP_001101129.1
Bos taurus (cattle)	SEMA5A	XP_005221710.1
Gallus gallus (chicken)	SEMA5A	XP_419016.3
Danio rerio (zebrafish)	sema5a	NP_001258599.1
Pan troglodytes (chimpanzee)	SEMA5A	XP_517627.2
Mus musculus (house mouse)	Sema5a	NP_033180.2
Homo sapiens (human)	SEMA5A	NP_003957.2
	SEMA5A	XP_002804352.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

G-quadruplexes as novel cis-elements controlling transcription during embryonic development.
David AP, Margarit E, Domizi P, Banchio C, Armas P, Calcaterra NB
Nucleic acids research44(9)4163-73(2016 05)

Modulation of tissue repair by regeneration enhancer elements.
Kang J, Hu J, Karra R, Dickson AL, Tornini VA, Nachtrab G, Gemberling M, Goldman JA, Black BL, Poss KD
Nature532(7598)201-6(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Semaphorin 5A is a bifunctional axon guidance cue for axial motoneurons in vivo.
Hilario JD, Rodino-Klapac LR, Wang C, Beattie CE
Developmental biology326(1)190-200(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sema5A is a bifunctional axon guidance cue for vertebrate motor axons in vivo. The TSR domain promotes growth of developing motor axons into the ventral myotome whereas the sema domain mediates repulsion
Title: Semaphorin 5A is a bifunctional axon guidance cue for axial motoneurons in vivo.

The following sema5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa43475	XM_021470194.1 Latest version!	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa35434	NM_001271670.1 Latest version!	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa28490	XM_017353840.1 Latest version!	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa28490	XM_005162619.4 Latest version!	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa28490	XM_005162619.3	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema5a ( XM_021470194.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325869.1 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43475
Clone ID Related Accession (Same CDS sequence)	XM_021470194.1
Accession Version	XM_021470194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-5A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA 
GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT 
GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG 
GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA 
GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG 
GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA 
ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT 
GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC 
ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA 
GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA 
TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC 
TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC 
GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG 
AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG 
GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC 
AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC 
GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC 
CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT 
CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG 
CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC 
AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG 
CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC 
CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT 
GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT 
AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA 
TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT 
CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT 
AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT 
CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC 
TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG 
ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC 
GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG 
CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA 
ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA 
TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA 
TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC 
TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG 
AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT 
GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG 
TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA 
CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC 
ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG 
ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT 
GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT 
CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT 
CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT 
GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA 
AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG 
AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC 
CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC 
AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGCCT 
GATGTTTCCG TTCTCTTGCC TTTGGACGAG CACCACATTT GCCAGCCTTT GTCTTCTAAC 
ACGGGTGTAG CGCGAGGCCT GGAGATTTTC TACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325869.1
CDS	465..3680
Translation	MAEGRPLARGLVLLHSLLLLMPRGVPCRGAIRALESPCHRAEHPLISHTDIEPWIHRFRAEGTVDYSQLTFDPGQNELIVGARNHLFRLHLEDLSLIQEAEWHCDEFTKGACFSRGKSEEECQNYIRVLLVNGDRLFTCGTNAFTPICTNRTLTNLTEVHDQISGMARCPYNPLHNSTALITSSGELYAATAMDFSGRDPAIYRSLGGLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGRTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEIPFNYNELQGTFFLPELELLYGIFTTNVNSIAASAVCAFNLSAITQVFSGPFKYQENSRSAWLPYPNPNPDFQCGTIDFGSYVNLTERNLQDAQKFILMHEVVQPVVPVPYFMEDNVRFSHVAVDVVQGKDMLYHIIYLATDYGTIKKVLSPLNQTTGSCLLDEIELFPLKKRQPIRSLLILHSHSELFVGVREQVIKIPLMRCNFHKSREACVGARDPYCGWDLVLKKCTTLEESVSMSQWEQSITRCPVRNVTVDGHYGAWSGWKTCSHSDGGSVGSCQCRTRACDSPSPQCGGQPCQGISVEVANCSRNGAWTPWTAWAPCSTSCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLPCPPHVYWSAWSPWERCTVPCGGGIQSRRRTCENGNECPGCSTEFQSCNTLPCPDLKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARVPEPGLLEVGRQRIEMRYCSSDGSTGCSTDGDLLRSGRITGLTVNGGWSVWGPWSQCSRDCSSGVRNRKRTCSNPKPKYGGITCLGPAQEFQECNTTPCPVDGSWSCWSPWSKCSVTCGGGHYMRTRSCNNPPPAYGGDICLGLHTEEALCNIQPCPESWSDWSEWSPCDSSGSQVRLRHCDVLFPTGNQCSGNSSETRACLPISNFIPETSVARSSQEERWCGDFNVFHMIAVGLSSSILGCLVTLLVYTYCQRYQQQSHDATVIHPISAAPLNTSISNHINKLDKYDSVEAIKPDVSVLLPLDEHHICQPLSSNTGVARGLEIFY

Target ORF information:

RefSeq Version	XM_021470194.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470194.1

ORF Insert Sequence:

ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA 
GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT 
GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG 
GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA 
GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG 
GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA 
ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT 
GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC 
ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA 
GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA 
TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC 
TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC 
GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG 
AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG 
GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC 
AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC 
GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC 
CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT 
CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG 
CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC 
AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG 
CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC 
CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT 
GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT 
AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA 
TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT 
CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT 
AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT 
CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC 
TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG 
ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC 
GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG 
CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA 
ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA 
TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA 
TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC 
TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG 
AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT 
GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG 
TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA 
CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC 
ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG 
ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT 
GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT 
CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT 
CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT 
GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA 
AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG 
AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC 
CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC 
AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGCCT 
GATGTTTCCG TTCTCTTGCC TTTGGACGAG CACCACATTT GCCAGCCTTT GTCTTCTAAC 
ACGGGTGTAG CGCGAGGCCT GGAGATTTTC TACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema5a ( NM_001271670.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001258599.1 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), mRNA.

Price & Availability↑

CloneID	ODa35434
Clone ID Related Accession (Same CDS sequence)	NM_001271670.1
Accession Version	NM_001271670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	semaphorin-5A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX323577.16, CR354550.8 and CT573359.10. On Nov 4, 2012 this sequence version replaced XM_001922538.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGTCCTGG TGCAGATTCC TTGTCCTCAT CTATTCTTGA ATGCTGCCCG TTTGTGTCAG 
GATCCAGTTC ACCTGGCTAA GGATGCAACA AAAACAGGCT CAATCTCCAG AATGCAAACC 
ATTCTGGTGG AAAGAAGGTG TCCGATGGTG CAGTCAAACT CTGGTTCAAA ACCAAAAAAA 
GAACCATCAA CAATATCCAG AGAGGAGGCA GAATGGCATT GTGATGAGTT TACCAAAGGA 
GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG GAGTGCCAGA ATTACATCCG TGTCCTCCTC 
GTCAATGGTG ACAGGCTGTT TACCTGCGGA ACAAATGCAT TCACTCCTAT TTGCACCAAT 
CGCACGCTGA CTAACCTGAC TGAGGTCCAT GATCAAATCA GTGGGATGGC ACGGTGCCCC 
TATAACCCTC TGCATAATTC CACCGCCCTC ATCACTTCCA GTGGAGAACT GTATGCTGCA 
ACTGCAATGG ACTTTTCAGG CAGAGACCCA GCCATCTACC GCAGCTTGGG AGGGCTTCCA 
CCTCTGCGTA CTGCTCAGTA CAACTCCAAA TGGCTCAATG AGCCCAACTT CGTCTCCTCC 
TATGACATCG GCAACTTCAC GTACTTCTTC TTCCGTGAAA ATGCTGTGGA GCACGACTGC 
GGCAGGACTG TTTTCTCTCG GGCTGCCCGC GTCTGCAAGA ATGATATCGG GGGCCGTTTC 
CTTCTGGAGG ACACCTGGAC TACCTTTATG AAAGCCCGGC TCAACTGCTC ACGGCCTGGC 
GAGATCCCAT TCAACTACAA TGAGTTGCAG GGAACCTTCT TTCTGCCTGA GCTCGAGCTC 
CTCTATGGGA TTTTCACCAC TAATGTTAAC AGTATTGCGG CCTCAGCAGT GTGTGCCTTC 
AATCTGAGCG CTATTACCCA AGTGTTCAGC GGCCCTTTCA AGTACCAAGA GAACTCGCGC 
TCTGCTTGGC TTCCTTACCC CAATCCTAAC CCCGACTTCC AGTGTGGTAC TATAGATTTT 
GGCTCGTATG TGAACTTAAC GGAGAGGAAT CTGCAGGATG CTCAGAAGTT CATCCTGATG 
CATGAGGTGG TGCAGCCTGT GGTTCCTGTG CCGTATTTCA TGGAGGACAA TGTGCGCTTC 
TCTCATGTGG CTGTGGACGT GGTGCAGGGC AAAGACATGC TTTACCACAT CATTTATCTG 
GCAACAGATT ACGGCACCAT TAAGAAGGTG CTCTCCCCTC TCAACCAGAC CACGGGCAGC 
TGCTTGCTGG ACGAGATTGA GCTTTTCCCC CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG 
CTCATCCTTC ACAGCCACAG TGAGCTATTT GTAGGAGTCA GAGAGCAGGT CATCAAAATC 
CCCCTGATGC GCTGCAACTT CCACAAGTCT AGAGAAGCCT GTGTGGGGGC CAGAGATCCA 
TACTGCGGCT GGGATCTGGT GCTCAAGAAA TGCACCACGC TGGAAGAGAG CGTCAGCATG 
AGCCAATGGG AGCAGAGCAT TACACGCTGT CCTGTGAGAA ACGTGACTGT AGATGGTCAT 
TATGGAGCCT GGTCAGGGTG GAAAACTTGT AGTCACAGTG ATGGTGGCAG TGTGGGTTCG 
TGCCAGTGTC GAACCCGAGC GTGTGACAGT CCAAGTCCTC AATGTGGCGG ACAGCCTTGC 
CAAGGAATCA GTGTTGAAGT GGCAAACTGC TCCAGAAATG GGGCATGGAC ACCGTGGACT 
GCCTGGGCTC CATGCAGCAC CAGCTGTGGG ATTGGATTCC AGGTTCGGCA GCGTTCCTGC 
AGTAACCCCA CTCCCAGACA TGGAGGACGC GTCTGTGTGG GCCAGAACCG AGAGGAGAGA 
TACTGCAATG AACACCTGCC GTGTCCTCCG CATGTCTATT GGTCAGCTTG GTCTCCTTGG 
GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT 
GGAAATGAAT GCCCTGGATG CAGCACTGAA TTCCAGTCCT GTAACACCCT CCCCTGTCCG 
GATCTGAAGA AGACCACGCC CTGGACCCCA TGGACCCCAG TGAATATCTC AGACAATGGT 
GGCCACTACG AGCAGCGCTT CAGATACACC TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG 
CTGGAAGTTG GACGACAGCG AATCGAAATG AGGTACTGCT CCAGCGACGG CTCCACGGGC 
TGCTCCACTG ACGGAGATTT GCTGCGTTCT GGACGTATCA CTGGATTGAC AGTAAATGGA 
GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA 
AATCGCAAAC GCACCTGCTC CAACCCCAAA CCCAAGTATG GAGGAATTAC CTGCCTCGGG 
CCAGCCCAAG AGTTCCAGGA GTGCAATACC ACACCATGTC CAGTGGACGG GAGCTGGTCG 
TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG ACATGCGGCG GAGGCCATTA CATGCGCACA 
CGCTCCTGTA ACAACCCTCC ACCTGCCTAT GGAGGTGACA TCTGCCTGGG TTTGCATACT 
GAAGAGGCCC TCTGCAACAT CCAGCCCTGT CCTGAGAGCT GGTCTGATTG GTCAGAATGG 
TCCCCCTGTG ACTCATCAGG GTCTCAGGTT CGCCTCCGGC ATTGTGACGT CTTGTTTCCC 
ACCGGCAACC AGTGCTCTGG CAACAGCAGT GAAACTCGTG CCTGTCTGCC CATCTCCAAC 
TTCATCCCAG AAACGTCTGT TGCACGTTCA AGTCAGGAGG AAAGATGGTG TGGAGACTTC 
AATGTGTTCC ACATGATCGC CGTGGGTCTG AGCAGCTCCA TACTCGGCTG CTTGGTCACT 
TTACTGGTGT ACACATACTG CCAGAGATAC CAGCAGCAAT CCCATGATGC CACAGTCATC 
CATCCTATCT CTGCTGCCCC GCTCAACACC AGCATCAGCA ACCATATCAA CAAACTGGAC 
AAGTATGACT CAGTTGAAGC CATTAAGGCT TTCAATAAGA ATAACTTGAT CCTGGAGGAA 
AGAAATAAGT ATTTCAACCC TCAGCTAGCA GGAAAAACAT ATACCAACGC ATATTTTACT 
GACGTCAACA CATACGACGA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258599.1
CDS	265..3411
Translation	MVLVQIPCPHLFLNAARLCQDPVHLAKDATKTGSISRMQTILVERRCPMVQSNSGSKPKKEPSTISREEAEWHCDEFTKGACFSRGKSEEECQNYIRVLLVNGDRLFTCGTNAFTPICTNRTLTNLTEVHDQISGMARCPYNPLHNSTALITSSGELYAATAMDFSGRDPAIYRSLGGLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGRTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEIPFNYNELQGTFFLPELELLYGIFTTNVNSIAASAVCAFNLSAITQVFSGPFKYQENSRSAWLPYPNPNPDFQCGTIDFGSYVNLTERNLQDAQKFILMHEVVQPVVPVPYFMEDNVRFSHVAVDVVQGKDMLYHIIYLATDYGTIKKVLSPLNQTTGSCLLDEIELFPLKKRQPIRSLLILHSHSELFVGVREQVIKIPLMRCNFHKSREACVGARDPYCGWDLVLKKCTTLEESVSMSQWEQSITRCPVRNVTVDGHYGAWSGWKTCSHSDGGSVGSCQCRTRACDSPSPQCGGQPCQGISVEVANCSRNGAWTPWTAWAPCSTSCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLPCPPHVYWSAWSPWERCTVPCGGGIQSRRRTCENGNECPGCSTEFQSCNTLPCPDLKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARVPEPGLLEVGRQRIEMRYCSSDGSTGCSTDGDLLRSGRITGLTVNGGWSVWGPWSQCSRDCSSGVRNRKRTCSNPKPKYGGITCLGPAQEFQECNTTPCPVDGSWSCWSPWSKCSVTCGGGHYMRTRSCNNPPPAYGGDICLGLHTEEALCNIQPCPESWSDWSEWSPCDSSGSQVRLRHCDVLFPTGNQCSGNSSETRACLPISNFIPETSVARSSQEERWCGDFNVFHMIAVGLSSSILGCLVTLLVYTYCQRYQQQSHDATVIHPISAAPLNTSISNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPQLAGKTYTNAYFTDVNTYDDF

Target ORF information:

RefSeq Version	NM_001271670.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271670.1

ORF Insert Sequence:

ATGGTCCTGG TGCAGATTCC TTGTCCTCAT CTATTCTTGA ATGCTGCCCG TTTGTGTCAG 
GATCCAGTTC ACCTGGCTAA GGATGCAACA AAAACAGGCT CAATCTCCAG AATGCAAACC 
ATTCTGGTGG AAAGAAGGTG TCCGATGGTG CAGTCAAACT CTGGTTCAAA ACCAAAAAAA 
GAACCATCAA CAATATCCAG AGAGGAGGCA GAATGGCATT GTGATGAGTT TACCAAAGGA 
GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG GAGTGCCAGA ATTACATCCG TGTCCTCCTC 
GTCAATGGTG ACAGGCTGTT TACCTGCGGA ACAAATGCAT TCACTCCTAT TTGCACCAAT 
CGCACGCTGA CTAACCTGAC TGAGGTCCAT GATCAAATCA GTGGGATGGC ACGGTGCCCC 
TATAACCCTC TGCATAATTC CACCGCCCTC ATCACTTCCA GTGGAGAACT GTATGCTGCA 
ACTGCAATGG ACTTTTCAGG CAGAGACCCA GCCATCTACC GCAGCTTGGG AGGGCTTCCA 
CCTCTGCGTA CTGCTCAGTA CAACTCCAAA TGGCTCAATG AGCCCAACTT CGTCTCCTCC 
TATGACATCG GCAACTTCAC GTACTTCTTC TTCCGTGAAA ATGCTGTGGA GCACGACTGC 
GGCAGGACTG TTTTCTCTCG GGCTGCCCGC GTCTGCAAGA ATGATATCGG GGGCCGTTTC 
CTTCTGGAGG ACACCTGGAC TACCTTTATG AAAGCCCGGC TCAACTGCTC ACGGCCTGGC 
GAGATCCCAT TCAACTACAA TGAGTTGCAG GGAACCTTCT TTCTGCCTGA GCTCGAGCTC 
CTCTATGGGA TTTTCACCAC TAATGTTAAC AGTATTGCGG CCTCAGCAGT GTGTGCCTTC 
AATCTGAGCG CTATTACCCA AGTGTTCAGC GGCCCTTTCA AGTACCAAGA GAACTCGCGC 
TCTGCTTGGC TTCCTTACCC CAATCCTAAC CCCGACTTCC AGTGTGGTAC TATAGATTTT 
GGCTCGTATG TGAACTTAAC GGAGAGGAAT CTGCAGGATG CTCAGAAGTT CATCCTGATG 
CATGAGGTGG TGCAGCCTGT GGTTCCTGTG CCGTATTTCA TGGAGGACAA TGTGCGCTTC 
TCTCATGTGG CTGTGGACGT GGTGCAGGGC AAAGACATGC TTTACCACAT CATTTATCTG 
GCAACAGATT ACGGCACCAT TAAGAAGGTG CTCTCCCCTC TCAACCAGAC CACGGGCAGC 
TGCTTGCTGG ACGAGATTGA GCTTTTCCCC CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG 
CTCATCCTTC ACAGCCACAG TGAGCTATTT GTAGGAGTCA GAGAGCAGGT CATCAAAATC 
CCCCTGATGC GCTGCAACTT CCACAAGTCT AGAGAAGCCT GTGTGGGGGC CAGAGATCCA 
TACTGCGGCT GGGATCTGGT GCTCAAGAAA TGCACCACGC TGGAAGAGAG CGTCAGCATG 
AGCCAATGGG AGCAGAGCAT TACACGCTGT CCTGTGAGAA ACGTGACTGT AGATGGTCAT 
TATGGAGCCT GGTCAGGGTG GAAAACTTGT AGTCACAGTG ATGGTGGCAG TGTGGGTTCG 
TGCCAGTGTC GAACCCGAGC GTGTGACAGT CCAAGTCCTC AATGTGGCGG ACAGCCTTGC 
CAAGGAATCA GTGTTGAAGT GGCAAACTGC TCCAGAAATG GGGCATGGAC ACCGTGGACT 
GCCTGGGCTC CATGCAGCAC CAGCTGTGGG ATTGGATTCC AGGTTCGGCA GCGTTCCTGC 
AGTAACCCCA CTCCCAGACA TGGAGGACGC GTCTGTGTGG GCCAGAACCG AGAGGAGAGA 
TACTGCAATG AACACCTGCC GTGTCCTCCG CATGTCTATT GGTCAGCTTG GTCTCCTTGG 
GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT 
GGAAATGAAT GCCCTGGATG CAGCACTGAA TTCCAGTCCT GTAACACCCT CCCCTGTCCG 
GATCTGAAGA AGACCACGCC CTGGACCCCA TGGACCCCAG TGAATATCTC AGACAATGGT 
GGCCACTACG AGCAGCGCTT CAGATACACC TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG 
CTGGAAGTTG GACGACAGCG AATCGAAATG AGGTACTGCT CCAGCGACGG CTCCACGGGC 
TGCTCCACTG ACGGAGATTT GCTGCGTTCT GGACGTATCA CTGGATTGAC AGTAAATGGA 
GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA 
AATCGCAAAC GCACCTGCTC CAACCCCAAA CCCAAGTATG GAGGAATTAC CTGCCTCGGG 
CCAGCCCAAG AGTTCCAGGA GTGCAATACC ACACCATGTC CAGTGGACGG GAGCTGGTCG 
TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG ACATGCGGCG GAGGCCATTA CATGCGCACA 
CGCTCCTGTA ACAACCCTCC ACCTGCCTAT GGAGGTGACA TCTGCCTGGG TTTGCATACT 
GAAGAGGCCC TCTGCAACAT CCAGCCCTGT CCTGAGAGCT GGTCTGATTG GTCAGAATGG 
TCCCCCTGTG ACTCATCAGG GTCTCAGGTT CGCCTCCGGC ATTGTGACGT CTTGTTTCCC 
ACCGGCAACC AGTGCTCTGG CAACAGCAGT GAAACTCGTG CCTGTCTGCC CATCTCCAAC 
TTCATCCCAG AAACGTCTGT TGCACGTTCA AGTCAGGAGG AAAGATGGTG TGGAGACTTC 
AATGTGTTCC ACATGATCGC CGTGGGTCTG AGCAGCTCCA TACTCGGCTG CTTGGTCACT 
TTACTGGTGT ACACATACTG CCAGAGATAC CAGCAGCAAT CCCATGATGC CACAGTCATC 
CATCCTATCT CTGCTGCCCC GCTCAACACC AGCATCAGCA ACCATATCAA CAAACTGGAC 
AAGTATGACT CAGTTGAAGC CATTAAGGCT TTCAATAAGA ATAACTTGAT CCTGGAGGAA 
AGAAATAAGT ATTTCAACCC TCAGCTAGCA GGAAAAACAT ATACCAACGC ATATTTTACT 
GACGTCAACA CATACGACGA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema5a ( XM_017353840.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209329.1 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa28490
Clone ID Related Accession (Same CDS sequence)	XM_017353840.1 , XM_005162619.3 , XM_005162619.4
Accession Version	XM_017353840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-5A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA 
GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT 
GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG 
GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA 
GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG 
GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA 
ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT 
GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC 
ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA 
GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA 
TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC 
TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC 
GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG 
AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG 
GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC 
AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC 
GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC 
CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT 
CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG 
CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC 
AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG 
CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC 
CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT 
GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT 
AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA 
TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT 
CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT 
AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT 
CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC 
TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG 
ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC 
GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG 
CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA 
ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA 
TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA 
TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC 
TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG 
AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT 
GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG 
TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA 
CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC 
ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG 
ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT 
GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT 
CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT 
CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT 
GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA 
AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG 
AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC 
CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC 
AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT 
TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA 
GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209329.1
CDS	112..3348
Translation	MAEGRPLARGLVLLHSLLLLMPRGVPCRGAIRALESPCHRAEHPLISHTDIEPWIHRFRAEGTVDYSQLTFDPGQNELIVGARNHLFRLHLEDLSLIQEAEWHCDEFTKGACFSRGKSEEECQNYIRVLLVNGDRLFTCGTNAFTPICTNRTLTNLTEVHDQISGMARCPYNPLHNSTALITSSGELYAATAMDFSGRDPAIYRSLGGLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGRTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEIPFNYNELQGTFFLPELELLYGIFTTNVNSIAASAVCAFNLSAITQVFSGPFKYQENSRSAWLPYPNPNPDFQCGTIDFGSYVNLTERNLQDAQKFILMHEVVQPVVPVPYFMEDNVRFSHVAVDVVQGKDMLYHIIYLATDYGTIKKVLSPLNQTTGSCLLDEIELFPLKKRQPIRSLLILHSHSELFVGVREQVIKIPLMRCNFHKSREACVGARDPYCGWDLVLKKCTTLEESVSMSQWEQSITRCPVRNVTVDGHYGAWSGWKTCSHSDGGSVGSCQCRTRACDSPSPQCGGQPCQGISVEVANCSRNGAWTPWTAWAPCSTSCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLPCPPHVYWSAWSPWERCTVPCGGGIQSRRRTCENGNECPGCSTEFQSCNTLPCPDLKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARVPEPGLLEVGRQRIEMRYCSSDGSTGCSTDGDLLRSGRITGLTVNGGWSVWGPWSQCSRDCSSGVRNRKRTCSNPKPKYGGITCLGPAQEFQECNTTPCPVDGSWSCWSPWSKCSVTCGGGHYMRTRSCNNPPPAYGGDICLGLHTEEALCNIQPCPESWSDWSEWSPCDSSGSQVRLRHCDVLFPTGNQCSGNSSETRACLPISNFIPETSVARSSQEERWCGDFNVFHMIAVGLSSSILGCLVTLLVYTYCQRYQQQSHDATVIHPISAAPLNTSISNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPQLAGKTYTNAYFTDVNTYDDF

Target ORF information:

RefSeq Version	XM_017353840.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353840.1

ORF Insert Sequence:

ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA 
GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT 
GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG 
GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA 
GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG 
GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA 
ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT 
GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC 
ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA 
GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA 
TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC 
TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC 
GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG 
AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG 
GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC 
AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC 
GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC 
CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT 
CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG 
CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC 
AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG 
CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC 
CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT 
GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT 
AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA 
TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT 
CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT 
AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT 
CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC 
TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG 
ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC 
GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG 
CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA 
ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA 
TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA 
TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC 
TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG 
AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT 
GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG 
TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA 
CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC 
ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG 
ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT 
GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT 
CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT 
CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT 
GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA 
AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG 
AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC 
CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC 
AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT 
TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA 
GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema5a ( XM_005162619.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162676.1 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28490
Clone ID Related Accession (Same CDS sequence)	XM_017353840.1 , XM_005162619.3 , XM_005162619.4
Accession Version	XM_005162619.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-5A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162619.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA 
GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT 
GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG 
GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA 
GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG 
GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA 
ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT 
GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC 
ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA 
GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA 
TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC 
TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC 
GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG 
AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG 
GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC 
AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC 
GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC 
CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT 
CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG 
CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC 
AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG 
CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC 
CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT 
GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT 
AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA 
TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT 
CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT 
AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT 
CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC 
TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG 
ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC 
GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG 
CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA 
ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA 
TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA 
TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC 
TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG 
AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT 
GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG 
TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA 
CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC 
ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG 
ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT 
GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT 
CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT 
CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT 
GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA 
AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG 
AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC 
CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC 
AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT 
TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA 
GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162676.1
CDS	442..3678
Translation	MAEGRPLARGLVLLHSLLLLMPRGVPCRGAIRALESPCHRAEHPLISHTDIEPWIHRFRAEGTVDYSQLTFDPGQNELIVGARNHLFRLHLEDLSLIQEAEWHCDEFTKGACFSRGKSEEECQNYIRVLLVNGDRLFTCGTNAFTPICTNRTLTNLTEVHDQISGMARCPYNPLHNSTALITSSGELYAATAMDFSGRDPAIYRSLGGLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGRTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEIPFNYNELQGTFFLPELELLYGIFTTNVNSIAASAVCAFNLSAITQVFSGPFKYQENSRSAWLPYPNPNPDFQCGTIDFGSYVNLTERNLQDAQKFILMHEVVQPVVPVPYFMEDNVRFSHVAVDVVQGKDMLYHIIYLATDYGTIKKVLSPLNQTTGSCLLDEIELFPLKKRQPIRSLLILHSHSELFVGVREQVIKIPLMRCNFHKSREACVGARDPYCGWDLVLKKCTTLEESVSMSQWEQSITRCPVRNVTVDGHYGAWSGWKTCSHSDGGSVGSCQCRTRACDSPSPQCGGQPCQGISVEVANCSRNGAWTPWTAWAPCSTSCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLPCPPHVYWSAWSPWERCTVPCGGGIQSRRRTCENGNECPGCSTEFQSCNTLPCPDLKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARVPEPGLLEVGRQRIEMRYCSSDGSTGCSTDGDLLRSGRITGLTVNGGWSVWGPWSQCSRDCSSGVRNRKRTCSNPKPKYGGITCLGPAQEFQECNTTPCPVDGSWSCWSPWSKCSVTCGGGHYMRTRSCNNPPPAYGGDICLGLHTEEALCNIQPCPESWSDWSEWSPCDSSGSQVRLRHCDVLFPTGNQCSGNSSETRACLPISNFIPETSVARSSQEERWCGDFNVFHMIAVGLSSSILGCLVTLLVYTYCQRYQQQSHDATVIHPISAAPLNTSISNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPQLAGKTYTNAYFTDVNTYDDF

Target ORF information:

RefSeq Version	XM_005162619.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162619.3

ORF Insert Sequence:

ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA 
GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT 
GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG 
GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA 
GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG 
GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA 
ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT 
GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC 
ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA 
GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA 
TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC 
TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC 
GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG 
AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG 
GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC 
AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC 
GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC 
CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT 
CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG 
CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC 
AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG 
CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC 
CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT 
GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT 
AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA 
TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT 
CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT 
AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT 
CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC 
TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG 
ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC 
GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG 
CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA 
ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA 
TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA 
TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC 
TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG 
AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT 
GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG 
TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA 
CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC 
ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG 
ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT 
GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT 
CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT 
CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT 
GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA 
AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG 
AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC 
CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC 
AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT 
TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA 
GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema5a ( XM_005162619.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162676.1 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X1, mRNA.

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CloneID	ODa28490
Clone ID Related Accession (Same CDS sequence)	XM_017353840.1 , XM_005162619.3 , XM_005162619.4
Accession Version	XM_005162619.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162619.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA 
GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT 
GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG 
GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA 
GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG 
GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA 
ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT 
GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC 
ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA 
GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA 
TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC 
TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC 
GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG 
AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG 
GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC 
AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC 
GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC 
CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT 
CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG 
CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC 
AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG 
CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC 
CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT 
GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT 
AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA 
TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT 
CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT 
AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT 
CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC 
TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG 
ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC 
GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG 
CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA 
ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA 
TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA 
TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC 
TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG 
AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT 
GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG 
TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA 
CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC 
ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG 
ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT 
GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT 
CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT 
CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT 
GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA 
AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG 
AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC 
CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC 
AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT 
TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA 
GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162676.1
CDS	463..3699
Translation	MAEGRPLARGLVLLHSLLLLMPRGVPCRGAIRALESPCHRAEHPLISHTDIEPWIHRFRAEGTVDYSQLTFDPGQNELIVGARNHLFRLHLEDLSLIQEAEWHCDEFTKGACFSRGKSEEECQNYIRVLLVNGDRLFTCGTNAFTPICTNRTLTNLTEVHDQISGMARCPYNPLHNSTALITSSGELYAATAMDFSGRDPAIYRSLGGLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGRTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEIPFNYNELQGTFFLPELELLYGIFTTNVNSIAASAVCAFNLSAITQVFSGPFKYQENSRSAWLPYPNPNPDFQCGTIDFGSYVNLTERNLQDAQKFILMHEVVQPVVPVPYFMEDNVRFSHVAVDVVQGKDMLYHIIYLATDYGTIKKVLSPLNQTTGSCLLDEIELFPLKKRQPIRSLLILHSHSELFVGVREQVIKIPLMRCNFHKSREACVGARDPYCGWDLVLKKCTTLEESVSMSQWEQSITRCPVRNVTVDGHYGAWSGWKTCSHSDGGSVGSCQCRTRACDSPSPQCGGQPCQGISVEVANCSRNGAWTPWTAWAPCSTSCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLPCPPHVYWSAWSPWERCTVPCGGGIQSRRRTCENGNECPGCSTEFQSCNTLPCPDLKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARVPEPGLLEVGRQRIEMRYCSSDGSTGCSTDGDLLRSGRITGLTVNGGWSVWGPWSQCSRDCSSGVRNRKRTCSNPKPKYGGITCLGPAQEFQECNTTPCPVDGSWSCWSPWSKCSVTCGGGHYMRTRSCNNPPPAYGGDICLGLHTEEALCNIQPCPESWSDWSEWSPCDSSGSQVRLRHCDVLFPTGNQCSGNSSETRACLPISNFIPETSVARSSQEERWCGDFNVFHMIAVGLSSSILGCLVTLLVYTYCQRYQQQSHDATVIHPISAAPLNTSISNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPQLAGKTYTNAYFTDVNTYDDF

Target ORF information:

RefSeq Version	XM_005162619.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (sema5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162619.4

ORF Insert Sequence:

ATGGCGGAGG GAAGGCCCCT GGCCCGCGGC CTCGTCCTGC TGCATAGCCT GCTGCTGCTG 
ATGCCCAGAG GAGTGCCCTG CCGGGGGGCC ATACGGGCCC TGGAGTCCCC CTGCCACAGA 
GCTGAACACC CGCTCATCTC TCACACAGAT ATTGAGCCGT GGATCCACCG GTTCAGAGCT 
GAAGGCACTG TCGATTATTC ACAGCTGACC TTTGACCCTG GCCAAAATGA ACTCATCGTG 
GGTGCAAGAA ATCATCTCTT CAGGCTACAT TTAGAGGATC TTTCACTGAT CCAGGAGGCA 
GAATGGCATT GTGATGAGTT TACCAAAGGA GCCTGTTTCA GTCGTGGGAA ATCAGAGGAG 
GAGTGCCAGA ATTACATCCG TGTCCTCCTC GTCAATGGTG ACAGGCTGTT TACCTGCGGA 
ACAAATGCAT TCACTCCTAT TTGCACCAAT CGCACGCTGA CTAACCTGAC TGAGGTCCAT 
GATCAAATCA GTGGGATGGC ACGGTGCCCC TATAACCCTC TGCATAATTC CACCGCCCTC 
ATCACTTCCA GTGGAGAACT GTATGCTGCA ACTGCAATGG ACTTTTCAGG CAGAGACCCA 
GCCATCTACC GCAGCTTGGG AGGGCTTCCA CCTCTGCGTA CTGCTCAGTA CAACTCCAAA 
TGGCTCAATG AGCCCAACTT CGTCTCCTCC TATGACATCG GCAACTTCAC GTACTTCTTC 
TTCCGTGAAA ATGCTGTGGA GCACGACTGC GGCAGGACTG TTTTCTCTCG GGCTGCCCGC 
GTCTGCAAGA ATGATATCGG GGGCCGTTTC CTTCTGGAGG ACACCTGGAC TACCTTTATG 
AAAGCCCGGC TCAACTGCTC ACGGCCTGGC GAGATCCCAT TCAACTACAA TGAGTTGCAG 
GGAACCTTCT TTCTGCCTGA GCTCGAGCTC CTCTATGGGA TTTTCACCAC TAATGTTAAC 
AGTATTGCGG CCTCAGCAGT GTGTGCCTTC AATCTGAGCG CTATTACCCA AGTGTTCAGC 
GGCCCTTTCA AGTACCAAGA GAACTCGCGC TCTGCTTGGC TTCCTTACCC CAATCCTAAC 
CCCGACTTCC AGTGTGGTAC TATAGATTTT GGCTCGTATG TGAACTTAAC GGAGAGGAAT 
CTGCAGGATG CTCAGAAGTT CATCCTGATG CATGAGGTGG TGCAGCCTGT GGTTCCTGTG 
CCGTATTTCA TGGAGGACAA TGTGCGCTTC TCTCATGTGG CTGTGGACGT GGTGCAGGGC 
AAAGACATGC TTTACCACAT CATTTATCTG GCAACAGATT ACGGCACCAT TAAGAAGGTG 
CTCTCCCCTC TCAACCAGAC CACGGGCAGC TGCTTGCTGG ACGAGATTGA GCTTTTCCCC 
CTGAAGAAGA GGCAGCCAAT TCGTAGCCTG CTCATCCTTC ACAGCCACAG TGAGCTATTT 
GTAGGAGTCA GAGAGCAGGT CATCAAAATC CCCCTGATGC GCTGCAACTT CCACAAGTCT 
AGAGAAGCCT GTGTGGGGGC CAGAGATCCA TACTGCGGCT GGGATCTGGT GCTCAAGAAA 
TGCACCACGC TGGAAGAGAG CGTCAGCATG AGCCAATGGG AGCAGAGCAT TACACGCTGT 
CCTGTGAGAA ACGTGACTGT AGATGGTCAT TATGGAGCCT GGTCAGGGTG GAAAACTTGT 
AGTCACAGTG ATGGTGGCAG TGTGGGTTCG TGCCAGTGTC GAACCCGAGC GTGTGACAGT 
CCAAGTCCTC AATGTGGCGG ACAGCCTTGC CAAGGAATCA GTGTTGAAGT GGCAAACTGC 
TCCAGAAATG GGGCATGGAC ACCGTGGACT GCCTGGGCTC CATGCAGCAC CAGCTGTGGG 
ATTGGATTCC AGGTTCGGCA GCGTTCCTGC AGTAACCCCA CTCCCAGACA TGGAGGACGC 
GTCTGTGTGG GCCAGAACCG AGAGGAGAGA TACTGCAATG AACACCTGCC GTGTCCTCCG 
CATGTCTATT GGTCAGCTTG GTCTCCTTGG GAGCGCTGCA CAGTTCCTTG CGGTGGAGGA 
ATCCAGTCAC GGCGAAGAAC GTGTGAGAAT GGAAATGAAT GCCCTGGATG CAGCACTGAA 
TTCCAGTCCT GTAACACCCT CCCCTGTCCG GATCTGAAGA AGACCACGCC CTGGACCCCA 
TGGACCCCAG TGAATATCTC AGACAATGGT GGCCACTACG AGCAGCGCTT CAGATACACC 
TGTAAAGCTC GAGTCCCAGA GCCCGGCCTG CTGGAAGTTG GACGACAGCG AATCGAAATG 
AGGTACTGCT CCAGCGACGG CTCCACGGGC TGCTCCACTG ACGGAGATTT GCTGCGTTCT 
GGACGTATCA CTGGATTGAC AGTAAATGGA GGCTGGTCCG TCTGGGGCCC CTGGAGCCAG 
TGTAGTCGAG ACTGTAGCTC AGGTGTCCGA AATCGCAAAC GCACCTGCTC CAACCCCAAA 
CCCAAGTATG GAGGAATTAC CTGCCTCGGG CCAGCCCAAG AGTTCCAGGA GTGCAATACC 
ACACCATGTC CAGTGGACGG GAGCTGGTCG TGCTGGTCAC CTTGGTCGAA ATGCTCAGTG 
ACATGCGGCG GAGGCCATTA CATGCGCACA CGCTCCTGTA ACAACCCTCC ACCTGCCTAT 
GGAGGTGACA TCTGCCTGGG TTTGCATACT GAAGAGGCCC TCTGCAACAT CCAGCCCTGT 
CCTGAGAGCT GGTCTGATTG GTCAGAATGG TCCCCCTGTG ACTCATCAGG GTCTCAGGTT 
CGCCTCCGGC ATTGTGACGT CTTGTTTCCC ACCGGCAACC AGTGCTCTGG CAACAGCAGT 
GAAACTCGTG CCTGTCTGCC CATCTCCAAC TTCATCCCAG AAACGTCTGT TGCACGTTCA 
AGTCAGGAGG AAAGATGGTG TGGAGACTTC AATGTGTTCC ACATGATCGC CGTGGGTCTG 
AGCAGCTCCA TACTCGGCTG CTTGGTCACT TTACTGGTGT ACACATACTG CCAGAGATAC 
CAGCAGCAAT CCCATGATGC CACAGTCATC CATCCTATCT CTGCTGCCCC GCTCAACACC 
AGCATCAGCA ACCATATCAA CAAACTGGAC AAGTATGACT CAGTTGAAGC CATTAAGGCT 
TTCAATAAGA ATAACTTGAT CCTGGAGGAA AGAAATAAGT ATTTCAACCC TCAGCTAGCA 
GGAAAAACAT ATACCAACGC ATATTTTACT GACGTCAACA CATACGACGA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

G-quadruplexes as novel cis-elements controlling transcription during embryonic development.
Nucleic acids research44(9)4163-73(2016 05)
David AP,Margarit E,Domizi P,Banchio C,Armas P,Calcaterra NB

Modulation of tissue repair by regeneration enhancer elements.
Nature532(7598)201-6(2016 Apr)
Kang J,Hu J,Karra R,Dickson AL,Tornini VA,Nachtrab G,Gemberling M,Goldman JA,Black BL,Poss KD

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Semaphorin 5A is a bifunctional axon guidance cue for axial motoneurons in vivo.
Developmental biology326(1)190-200(2009 Feb)
Hilario JD,Rodino-Klapac LR,Wang C,Beattie CE

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sema5a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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