map3k10 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	map3k10
Entrez Gene ID	560932
Full Name	mitogen-activated protein kinase kinase kinase 10
Synonyms	si:ch211-238g23.2
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 10 Names mitogen-activated protein kinase kinase kinase 10
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_684332.7	XP_689424.4	mitogen-activated protein kinase kinase kinase 10
XM_684332.8	XP_689424.4	mitogen-activated protein kinase kinase kinase 10

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAP3K10	NP_002437.2
	MAP3K10	XP_001091736.2
Mus musculus (house mouse)	Map3k10	NP_001074761.1
Canis lupus familiaris (dog)	MAP3K10	XP_005616941.1
Rattus norvegicus (Norway rat)	Map3k10	NP_001178550.1
Xenopus tropicalis (tropical clawed frog)	map3k10	XP_002938858.2
Danio rerio (zebrafish)	map3k10	XP_689424.4

Gene Ontology

The following map3k10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map3k10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23506	XM_684332.8 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa23506	XM_684332.7	Danio rerio mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

map3k10 ( XM_684332.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_689424.4 Danio rerio mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.

Price & Availability↑

CloneID	ODa23506
Clone ID Related Accession (Same CDS sequence)	XM_684332.7 , XM_684332.8
Accession Version	XM_684332.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitogen-activated protein kinase kinase kinase 10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_684332.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAC ATACGAGGCT GAGTGGAGTA GGAGCTGGAG GTGGTGGCAG TGCTGCCCCA 
CTACCTTGCA TCAGCAGTGG GGGGTCCTGT GGACACCTGG TTCCCCCTGA TACTCAAGCA 
AATACAAATT CAACACCTTT CCTGATAAGC ATCAACAGCA GCCGGAACTC CTCTACTATT 
CCCTCCTCAG TGGGCTCCCC AGATTCCCAC CTACCCCATC ATCATGAAAG CAGCAACACA 
TACTGGACCG CTGTCTTCGA TTATGAGGCT ACAGCAGACG AGGAGCTGAC CTTGCGGCGT 
GGCGACCTAC TGGAGGTGCT GTCCAAAGAC TCCAAAGTGT CTGGAGATGA GGGATGGTGG 
ACTGGAAAGA TCCAAGACAA GGTGGGCATA TTCCCTTGTA ATTATGTAAC CCAACGGGAT 
TCCAGCTATC CAGCGTTACC ACCGGAAGCT CTGGTTGGTG GAGATTCACC ACTGGAAATT 
GATTTCTTAG AGCTGCGATT GGAGGAAGTG ATTGGTGCTG GCGGCTTTGG AAAGGTGTAT 
AAGGGTGTAT GGCGAGCTGA GGAAGTGGCT GTCAAAGCAG CTCGTCAGGA CCCAGATGAG 
GACATCAGTG CCACGGCAGA GAATGTGAGG CAGGAGGCCA GACTCTTCTG GATGCTGCGT 
CACCGCAACA TCATCGCTCT ACGAGGTGTT TGTCTGCGGG AGCCAAATCT TTGCCTTGTT 
ATGGAATATG CCCGTGGTGG GGCGCTTAAC CGGGCACTTG CTGGCAAGAA GGTGCCTCCT 
CGGGTACTGG TGAATTGGGC TGTGCAGATT GCAACTGGAA TGGACTACTT GCACAACCAG 
ACCTTTGTGC CAATCATCCA TCGAGACCTC AAATCCAGCA ACATTCTGAT TCTGGAGCCT 
GTGGAGAGAG ATGACCTGAG TGGAAAGACA CTGAAGATCA CAGACTTTGG TCTTGCTCGA 
GAATGGCATC GCACCACCAA GATGAGCGCA GCGGGCACGT ATGCCTGGAT GGCTCCAGAG 
GTCATCAAGC TCTCCCTGTT CTCCAAAAGC AGCGATGTGT GGAGTTTTGG AGTGTTATTG 
TGGGAGCTGC TGACTGGTGA AGTGCCATAC CGTGAGATCG ACGCTTTGGC TGTGGCCTAT 
GGAGTTGCCA TGAACAAGTT GACTCTTCCC ATCCCCTCCA CCTGCCCTGA AGCCTTTGCC 
CAGCTGCTGG GAGAGTGTTG GTGTCCTAAC CCACGTGGAC GACCTGCTTT TGGGAGTATC 
CTGAAGAGGC TGGAGGACAT CGAGCAGTCG GCCATGTTCC AGATGCCCCT GGAGTCTTTC 
CATTCCCTGC AGGAGGACTG GAGGCTGGAG ATTCAGCAAA TGTTTGATGA ACTACGAGCA 
AAGGAGAAGG AGTTGCGATC CTGGGAGGAA GCTCTGGCCC GGGCAGCGGA GGAGCAGAGG 
GAGCAGGAGG AGCATCTGAG GAGACGCGAG CAGGAGTTAG CAGAGCGAGA GATCGACATA 
GTGGAGAGAG AGCTCAACAT CATCATCCAT CAGATGTACC AAGAGAAACC GCAGGTCAAA 
AAGCGCAAAG GCAACTTCAA AAAGAGCCGT CTGCTCAAGC TCGGCAGAGA CAGCAACTGC 
ATCAGTCTGC CATCTGGTTT TGAGCATAAG ATCACAGTGC AAGCTTCGCC CAGTGTGGAC 
AAGAGGAAAT GTCAGAGTAG TGAAAGCACC ACTCCTCCAG CCAGCCCAGG GGTCATTCCT 
CGCCTCAGAG CCTTTCGCTT GACACCCAGT GATGGGAGGA AGACGTGGGG CCGGACGGCA 
GTGTGTAAGA AAGAGGAGCC GGGGACCAAA AAGAAGGGCC GAACATGGGG TCCGAGCTCC 
ACACATCAGC GTGAGAGAGT CGGTGGAGAG GACAGGCTAA AGTCTCTTGG GGAGGGCAAA 
GTGTGGTCCT CCAGTGCTCC AAATCTGGGG AAGTCCCCAA AACATGCTCC TATGACAGCT 
GGATTCTCAA GCCTAAATGA AATGGAGGAG CACTGTGAGT TTGATGACAG TTCTCCAGGG 
CGCCTGGCTC CTGATCTGGG CAGTAACGGT GCGCTGGAAG ACGTGGGCTC GGGATCAGCA 
GGTTTAGGGT CAGGAATAGG CTCTCAGGAT TCAGTTTGCC GCTGCAGCCA GAGGAAGAAG 
AGTGACCTGC TGTTGCTGGG CTGTGCCTCT CTACTGGCCG TTGTGGGACT CGGTTCTGAC 
ATCCTCCAGT TGGGACGACA ACAGATGAAT CAAGATGAGC AGGACGTGCG AGAGGAGCAG 
CGCAAGAAAA AAGAGGGCCT GTTCCAGCGC ACGGGTCGAT TCAGACGCAG CACTTCTCCA 
CCCAGCCGCA CACTCTCCCT TTCCCTGTCC CACAACCGCC ATCACGACTC CATCTTGCCC 
AGCATGGACC CGTCTCCATC AGTCACCCTC CTGTCCCTGT CCTCCCTCTC TGACTGTAAC 
TCCACCAAAT CCCTGTTGCC TTCTGATCCT GATGACTTTG CCCCGACCCC TAGTAATGCC 
CCTCCGGTGG TGTCACCCGC ACCGGCATTA AACCCTCTGC TGGACCTGAG AGCGGAGAGC 
TTTAAAAGAG AGCCCAATCA GTCCCTCACT CCCACCCATG TCTCTGCAAC CATGGCATTA 
AACCGCGGGC ACCGGCGGAC GCCCTCTGAT GGAGCCATTC GTCCTCGAGC GCAGACGGTT 
GTGGGGCATC GCCGGACCCC TTCTGATGGG AGTATGCCCT TCCCGCCTGC TCATGCCCCT 
ACTATTACAC CATATACAGG TTCTCGAGAA ACTCTTGACA TTCCACGTCT GCCTGACCCG 
TCTATGATGT TCCCCTCAGC ACTTCGGCGC AAACCTGCAC CTCCCATCCC CACACCTGAG 
CCAGATAGCT ATACGGGCAC TCTAGAGCGT CCCAAAACCC TGGAGTTTGC TCCTCGTCCC 
CGTCCCACCC CAGCCCGTGC CCGTCCAGAC CCCTGGAAAC TGTCTTCTCT GTCCCAGACA 
CTCAGCTCTT CTCCGGGCAG CAGCTGCGAC AGTCCGCTGG GTTCGGGTGA CAGCGGTGTC 
AGTGCCGGCA CCGGCGGTGT CCATCAGAGC CTCCTGGACA TGGACATGGA GGGCCAGAGC 
CAGGACAGCA CGGTCCCCCT GTGCGGACAA CGCGCAAAGG CCACTCTCTG CGCTCAGCAT 
TCCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_689424.4
CDS	1823..5011
Translation	MDPHTRLSGVGAGGGGSAAPLPCISSGGSCGHLVPPDTQANTNSTPFLISINSSRNSSTIPSSVGSPDSHLPHHHESSNTYWTAVFDYEATADEELTLRRGDLLEVLSKDSKVSGDEGWWTGKIQDKVGIFPCNYVTQRDSSYPALPPEALVGGDSPLEIDFLELRLEEVIGAGGFGKVYKGVWRAEEVAVKAARQDPDEDISATAENVRQEARLFWMLRHRNIIALRGVCLREPNLCLVMEYARGGALNRALAGKKVPPRVLVNWAVQIATGMDYLHNQTFVPIIHRDLKSSNILILEPVERDDLSGKTLKITDFGLAREWHRTTKMSAAGTYAWMAPEVIKLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEAFAQLLGECWCPNPRGRPAFGSILKRLEDIEQSAMFQMPLESFHSLQEDWRLEIQQMFDELRAKEKELRSWEEALARAAEEQREQEEHLRRREQELAEREIDIVERELNIIIHQMYQEKPQVKKRKGNFKKSRLLKLGRDSNCISLPSGFEHKITVQASPSVDKRKCQSSESTTPPASPGVIPRLRAFRLTPSDGRKTWGRTAVCKKEEPGTKKKGRTWGPSSTHQRERVGGEDRLKSLGEGKVWSSSAPNLGKSPKHAPMTAGFSSLNEMEEHCEFDDSSPGRLAPDLGSNGALEDVGSGSAGLGSGIGSQDSVCRCSQRKKSDLLLLGCASLLAVVGLGSDILQLGRQQMNQDEQDVREEQRKKKEGLFQRTGRFRRSTSPPSRTLSLSLSHNRHHDSILPSMDPSPSVTLLSLSSLSDCNSTKSLLPSDPDDFAPTPSNAPPVVSPAPALNPLLDLRAESFKREPNQSLTPTHVSATMALNRGHRRTPSDGAIRPRAQTVVGHRRTPSDGSMPFPPAHAPTITPYTGSRETLDIPRLPDPSMMFPSALRRKPAPPIPTPEPDSYTGTLERPKTLEFAPRPRPTPARARPDPWKLSSLSQTLSSSPGSSCDSPLGSGDSGVSAGTGGVHQSLLDMDMEGQSQDSTVPLCGQRAKATLCAQHSS

Target ORF information:

RefSeq Version	XM_684332.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_684332.7

ORF Insert Sequence:

ATGGATCCAC ATACGAGGCT GAGTGGAGTA GGAGCTGGAG GTGGTGGCAG TGCTGCCCCA 
CTACCTTGCA TCAGCAGTGG GGGGTCCTGT GGACACCTGG TTCCCCCTGA TACTCAAGCA 
AATACAAATT CAACACCTTT CCTGATAAGC ATCAACAGCA GCCGGAACTC CTCTACTATT 
CCCTCCTCAG TGGGCTCCCC AGATTCCCAC CTACCCCATC ATCATGAAAG CAGCAACACA 
TACTGGACCG CTGTCTTCGA TTATGAGGCT ACAGCAGACG AGGAGCTGAC CTTGCGGCGT 
GGCGACCTAC TGGAGGTGCT GTCCAAAGAC TCCAAAGTGT CTGGAGATGA GGGATGGTGG 
ACTGGAAAGA TCCAAGACAA GGTGGGCATA TTCCCTTGTA ATTATGTAAC CCAACGGGAT 
TCCAGCTATC CAGCGTTACC ACCGGAAGCT CTGGTTGGTG GAGATTCACC ACTGGAAATT 
GATTTCTTAG AGCTGCGATT GGAGGAAGTG ATTGGTGCTG GCGGCTTTGG AAAGGTGTAT 
AAGGGTGTAT GGCGAGCTGA GGAAGTGGCT GTCAAAGCAG CTCGTCAGGA CCCAGATGAG 
GACATCAGTG CCACGGCAGA GAATGTGAGG CAGGAGGCCA GACTCTTCTG GATGCTGCGT 
CACCGCAACA TCATCGCTCT ACGAGGTGTT TGTCTGCGGG AGCCAAATCT TTGCCTTGTT 
ATGGAATATG CCCGTGGTGG GGCGCTTAAC CGGGCACTTG CTGGCAAGAA GGTGCCTCCT 
CGGGTACTGG TGAATTGGGC TGTGCAGATT GCAACTGGAA TGGACTACTT GCACAACCAG 
ACCTTTGTGC CAATCATCCA TCGAGACCTC AAATCCAGCA ACATTCTGAT TCTGGAGCCT 
GTGGAGAGAG ATGACCTGAG TGGAAAGACA CTGAAGATCA CAGACTTTGG TCTTGCTCGA 
GAATGGCATC GCACCACCAA GATGAGCGCA GCGGGCACGT ATGCCTGGAT GGCTCCAGAG 
GTCATCAAGC TCTCCCTGTT CTCCAAAAGC AGCGATGTGT GGAGTTTTGG AGTGTTATTG 
TGGGAGCTGC TGACTGGTGA AGTGCCATAC CGTGAGATCG ACGCTTTGGC TGTGGCCTAT 
GGAGTTGCCA TGAACAAGTT GACTCTTCCC ATCCCCTCCA CCTGCCCTGA AGCCTTTGCC 
CAGCTGCTGG GAGAGTGTTG GTGTCCTAAC CCACGTGGAC GACCTGCTTT TGGGAGTATC 
CTGAAGAGGC TGGAGGACAT CGAGCAGTCG GCCATGTTCC AGATGCCCCT GGAGTCTTTC 
CATTCCCTGC AGGAGGACTG GAGGCTGGAG ATTCAGCAAA TGTTTGATGA ACTACGAGCA 
AAGGAGAAGG AGTTGCGATC CTGGGAGGAA GCTCTGGCCC GGGCAGCGGA GGAGCAGAGG 
GAGCAGGAGG AGCATCTGAG GAGACGCGAG CAGGAGTTAG CAGAGCGAGA GATCGACATA 
GTGGAGAGAG AGCTCAACAT CATCATCCAT CAGATGTACC AAGAGAAACC GCAGGTCAAA 
AAGCGCAAAG GCAACTTCAA AAAGAGCCGT CTGCTCAAGC TCGGCAGAGA CAGCAACTGC 
ATCAGTCTGC CATCTGGTTT TGAGCATAAG ATCACAGTGC AAGCTTCGCC CAGTGTGGAC 
AAGAGGAAAT GTCAGAGTAG TGAAAGCACC ACTCCTCCAG CCAGCCCAGG GGTCATTCCT 
CGCCTCAGAG CCTTTCGCTT GACACCCAGT GATGGGAGGA AGACGTGGGG CCGGACGGCA 
GTGTGTAAGA AAGAGGAGCC GGGGACCAAA AAGAAGGGCC GAACATGGGG TCCGAGCTCC 
ACACATCAGC GTGAGAGAGT CGGTGGAGAG GACAGGCTAA AGTCTCTTGG GGAGGGCAAA 
GTGTGGTCCT CCAGTGCTCC AAATCTGGGG AAGTCCCCAA AACATGCTCC TATGACAGCT 
GGATTCTCAA GCCTAAATGA AATGGAGGAG CACTGTGAGT TTGATGACAG TTCTCCAGGG 
CGCCTGGCTC CTGATCTGGG CAGTAACGGT GCGCTGGAAG ACGTGGGCTC GGGATCAGCA 
GGTTTAGGGT CAGGAATAGG CTCTCAGGAT TCAGTTTGCC GCTGCAGCCA GAGGAAGAAG 
AGTGACCTGC TGTTGCTGGG CTGTGCCTCT CTACTGGCCG TTGTGGGACT CGGTTCTGAC 
ATCCTCCAGT TGGGACGACA ACAGATGAAT CAAGATGAGC AGGACGTGCG AGAGGAGCAG 
CGCAAGAAAA AAGAGGGCCT GTTCCAGCGC ACGGGTCGAT TCAGACGCAG CACTTCTCCA 
CCCAGCCGCA CACTCTCCCT TTCCCTGTCC CACAACCGCC ATCACGACTC CATCTTGCCC 
AGCATGGACC CGTCTCCATC AGTCACCCTC CTGTCCCTGT CCTCCCTCTC TGACTGTAAC 
TCCACCAAAT CCCTGTTGCC TTCTGATCCT GATGACTTTG CCCCGACCCC TAGTAATGCC 
CCTCCGGTGG TGTCACCCGC ACCGGCATTA AACCCTCTGC TGGACCTGAG AGCGGAGAGC 
TTTAAAAGAG AGCCCAATCA GTCCCTCACT CCCACCCATG TCTCTGCAAC CATGGCATTA 
AACCGCGGGC ACCGGCGGAC GCCCTCTGAT GGAGCCATTC GTCCTCGAGC GCAGACGGTT 
GTGGGGCATC GCCGGACCCC TTCTGATGGG AGTATGCCCT TCCCGCCTGC TCATGCCCCT 
ACTATTACAC CATATACAGG TTCTCGAGAA ACTCTTGACA TTCCACGTCT GCCTGACCCG 
TCTATGATGT TCCCCTCAGC ACTTCGGCGC AAACCTGCAC CTCCCATCCC CACACCTGAG 
CCAGATAGCT ATACGGGCAC TCTAGAGCGT CCCAAAACCC TGGAGTTTGC TCCTCGTCCC 
CGTCCCACCC CAGCCCGTGC CCGTCCAGAC CCCTGGAAAC TGTCTTCTCT GTCCCAGACA 
CTCAGCTCTT CTCCGGGCAG CAGCTGCGAC AGTCCGCTGG GTTCGGGTGA CAGCGGTGTC 
AGTGCCGGCA CCGGCGGTGT CCATCAGAGC CTCCTGGACA TGGACATGGA GGGCCAGAGC 
CAGGACAGCA CGGTCCCCCT GTGCGGACAA CGCGCAAAGG CCACTCTCTG CGCTCAGCAT 
TCCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k10 ( XM_684332.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_689424.4 Danio rerio mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.

Price & Availability↑

CloneID	ODa23506
Clone ID Related Accession (Same CDS sequence)	XM_684332.7 , XM_684332.8
Accession Version	XM_684332.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase 10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_684332.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAC ATACGAGGCT GAGTGGAGTA GGAGCTGGAG GTGGTGGCAG TGCTGCCCCA 
CTACCTTGCA TCAGCAGTGG GGGGTCCTGT GGACACCTGG TTCCCCCTGA TACTCAAGCA 
AATACAAATT CAACACCTTT CCTGATAAGC ATCAACAGCA GCCGGAACTC CTCTACTATT 
CCCTCCTCAG TGGGCTCCCC AGATTCCCAC CTACCCCATC ATCATGAAAG CAGCAACACA 
TACTGGACCG CTGTCTTCGA TTATGAGGCT ACAGCAGACG AGGAGCTGAC CTTGCGGCGT 
GGCGACCTAC TGGAGGTGCT GTCCAAAGAC TCCAAAGTGT CTGGAGATGA GGGATGGTGG 
ACTGGAAAGA TCCAAGACAA GGTGGGCATA TTCCCTTGTA ATTATGTAAC CCAACGGGAT 
TCCAGCTATC CAGCGTTACC ACCGGAAGCT CTGGTTGGTG GAGATTCACC ACTGGAAATT 
GATTTCTTAG AGCTGCGATT GGAGGAAGTG ATTGGTGCTG GCGGCTTTGG AAAGGTGTAT 
AAGGGTGTAT GGCGAGCTGA GGAAGTGGCT GTCAAAGCAG CTCGTCAGGA CCCAGATGAG 
GACATCAGTG CCACGGCAGA GAATGTGAGG CAGGAGGCCA GACTCTTCTG GATGCTGCGT 
CACCGCAACA TCATCGCTCT ACGAGGTGTT TGTCTGCGGG AGCCAAATCT TTGCCTTGTT 
ATGGAATATG CCCGTGGTGG GGCGCTTAAC CGGGCACTTG CTGGCAAGAA GGTGCCTCCT 
CGGGTACTGG TGAATTGGGC TGTGCAGATT GCAACTGGAA TGGACTACTT GCACAACCAG 
ACCTTTGTGC CAATCATCCA TCGAGACCTC AAATCCAGCA ACATTCTGAT TCTGGAGCCT 
GTGGAGAGAG ATGACCTGAG TGGAAAGACA CTGAAGATCA CAGACTTTGG TCTTGCTCGA 
GAATGGCATC GCACCACCAA GATGAGCGCA GCGGGCACGT ATGCCTGGAT GGCTCCAGAG 
GTCATCAAGC TCTCCCTGTT CTCCAAAAGC AGCGATGTGT GGAGTTTTGG AGTGTTATTG 
TGGGAGCTGC TGACTGGTGA AGTGCCATAC CGTGAGATCG ACGCTTTGGC TGTGGCCTAT 
GGAGTTGCCA TGAACAAGTT GACTCTTCCC ATCCCCTCCA CCTGCCCTGA AGCCTTTGCC 
CAGCTGCTGG GAGAGTGTTG GTGTCCTAAC CCACGTGGAC GACCTGCTTT TGGGAGTATC 
CTGAAGAGGC TGGAGGACAT CGAGCAGTCG GCCATGTTCC AGATGCCCCT GGAGTCTTTC 
CATTCCCTGC AGGAGGACTG GAGGCTGGAG ATTCAGCAAA TGTTTGATGA ACTACGAGCA 
AAGGAGAAGG AGTTGCGATC CTGGGAGGAA GCTCTGGCCC GGGCAGCGGA GGAGCAGAGG 
GAGCAGGAGG AGCATCTGAG GAGACGCGAG CAGGAGTTAG CAGAGCGAGA GATCGACATA 
GTGGAGAGAG AGCTCAACAT CATCATCCAT CAGATGTACC AAGAGAAACC GCAGGTCAAA 
AAGCGCAAAG GCAACTTCAA AAAGAGCCGT CTGCTCAAGC TCGGCAGAGA CAGCAACTGC 
ATCAGTCTGC CATCTGGTTT TGAGCATAAG ATCACAGTGC AAGCTTCGCC CAGTGTGGAC 
AAGAGGAAAT GTCAGAGTAG TGAAAGCACC ACTCCTCCAG CCAGCCCAGG GGTCATTCCT 
CGCCTCAGAG CCTTTCGCTT GACACCCAGT GATGGGAGGA AGACGTGGGG CCGGACGGCA 
GTGTGTAAGA AAGAGGAGCC GGGGACCAAA AAGAAGGGCC GAACATGGGG TCCGAGCTCC 
ACACATCAGC GTGAGAGAGT CGGTGGAGAG GACAGGCTAA AGTCTCTTGG GGAGGGCAAA 
GTGTGGTCCT CCAGTGCTCC AAATCTGGGG AAGTCCCCAA AACATGCTCC TATGACAGCT 
GGATTCTCAA GCCTAAATGA AATGGAGGAG CACTGTGAGT TTGATGACAG TTCTCCAGGG 
CGCCTGGCTC CTGATCTGGG CAGTAACGGT GCGCTGGAAG ACGTGGGCTC GGGATCAGCA 
GGTTTAGGGT CAGGAATAGG CTCTCAGGAT TCAGTTTGCC GCTGCAGCCA GAGGAAGAAG 
AGTGACCTGC TGTTGCTGGG CTGTGCCTCT CTACTGGCCG TTGTGGGACT CGGTTCTGAC 
ATCCTCCAGT TGGGACGACA ACAGATGAAT CAAGATGAGC AGGACGTGCG AGAGGAGCAG 
CGCAAGAAAA AAGAGGGCCT GTTCCAGCGC ACGGGTCGAT TCAGACGCAG CACTTCTCCA 
CCCAGCCGCA CACTCTCCCT TTCCCTGTCC CACAACCGCC ATCACGACTC CATCTTGCCC 
AGCATGGACC CGTCTCCATC AGTCACCCTC CTGTCCCTGT CCTCCCTCTC TGACTGTAAC 
TCCACCAAAT CCCTGTTGCC TTCTGATCCT GATGACTTTG CCCCGACCCC TAGTAATGCC 
CCTCCGGTGG TGTCACCCGC ACCGGCATTA AACCCTCTGC TGGACCTGAG AGCGGAGAGC 
TTTAAAAGAG AGCCCAATCA GTCCCTCACT CCCACCCATG TCTCTGCAAC CATGGCATTA 
AACCGCGGGC ACCGGCGGAC GCCCTCTGAT GGAGCCATTC GTCCTCGAGC GCAGACGGTT 
GTGGGGCATC GCCGGACCCC TTCTGATGGG AGTATGCCCT TCCCGCCTGC TCATGCCCCT 
ACTATTACAC CATATACAGG TTCTCGAGAA ACTCTTGACA TTCCACGTCT GCCTGACCCG 
TCTATGATGT TCCCCTCAGC ACTTCGGCGC AAACCTGCAC CTCCCATCCC CACACCTGAG 
CCAGATAGCT ATACGGGCAC TCTAGAGCGT CCCAAAACCC TGGAGTTTGC TCCTCGTCCC 
CGTCCCACCC CAGCCCGTGC CCGTCCAGAC CCCTGGAAAC TGTCTTCTCT GTCCCAGACA 
CTCAGCTCTT CTCCGGGCAG CAGCTGCGAC AGTCCGCTGG GTTCGGGTGA CAGCGGTGTC 
AGTGCCGGCA CCGGCGGTGT CCATCAGAGC CTCCTGGACA TGGACATGGA GGGCCAGAGC 
CAGGACAGCA CGGTCCCCCT GTGCGGACAA CGCGCAAAGG CCACTCTCTG CGCTCAGCAT 
TCCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_689424.4
CDS	1837..5025
Translation	MDPHTRLSGVGAGGGGSAAPLPCISSGGSCGHLVPPDTQANTNSTPFLISINSSRNSSTIPSSVGSPDSHLPHHHESSNTYWTAVFDYEATADEELTLRRGDLLEVLSKDSKVSGDEGWWTGKIQDKVGIFPCNYVTQRDSSYPALPPEALVGGDSPLEIDFLELRLEEVIGAGGFGKVYKGVWRAEEVAVKAARQDPDEDISATAENVRQEARLFWMLRHRNIIALRGVCLREPNLCLVMEYARGGALNRALAGKKVPPRVLVNWAVQIATGMDYLHNQTFVPIIHRDLKSSNILILEPVERDDLSGKTLKITDFGLAREWHRTTKMSAAGTYAWMAPEVIKLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEAFAQLLGECWCPNPRGRPAFGSILKRLEDIEQSAMFQMPLESFHSLQEDWRLEIQQMFDELRAKEKELRSWEEALARAAEEQREQEEHLRRREQELAEREIDIVERELNIIIHQMYQEKPQVKKRKGNFKKSRLLKLGRDSNCISLPSGFEHKITVQASPSVDKRKCQSSESTTPPASPGVIPRLRAFRLTPSDGRKTWGRTAVCKKEEPGTKKKGRTWGPSSTHQRERVGGEDRLKSLGEGKVWSSSAPNLGKSPKHAPMTAGFSSLNEMEEHCEFDDSSPGRLAPDLGSNGALEDVGSGSAGLGSGIGSQDSVCRCSQRKKSDLLLLGCASLLAVVGLGSDILQLGRQQMNQDEQDVREEQRKKKEGLFQRTGRFRRSTSPPSRTLSLSLSHNRHHDSILPSMDPSPSVTLLSLSSLSDCNSTKSLLPSDPDDFAPTPSNAPPVVSPAPALNPLLDLRAESFKREPNQSLTPTHVSATMALNRGHRRTPSDGAIRPRAQTVVGHRRTPSDGSMPFPPAHAPTITPYTGSRETLDIPRLPDPSMMFPSALRRKPAPPIPTPEPDSYTGTLERPKTLEFAPRPRPTPARARPDPWKLSSLSQTLSSSPGSSCDSPLGSGDSGVSAGTGGVHQSLLDMDMEGQSQDSTVPLCGQRAKATLCAQHSS

Target ORF information:

RefSeq Version	XM_684332.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_684332.8

ORF Insert Sequence:

ATGGATCCAC ATACGAGGCT GAGTGGAGTA GGAGCTGGAG GTGGTGGCAG TGCTGCCCCA 
CTACCTTGCA TCAGCAGTGG GGGGTCCTGT GGACACCTGG TTCCCCCTGA TACTCAAGCA 
AATACAAATT CAACACCTTT CCTGATAAGC ATCAACAGCA GCCGGAACTC CTCTACTATT 
CCCTCCTCAG TGGGCTCCCC AGATTCCCAC CTACCCCATC ATCATGAAAG CAGCAACACA 
TACTGGACCG CTGTCTTCGA TTATGAGGCT ACAGCAGACG AGGAGCTGAC CTTGCGGCGT 
GGCGACCTAC TGGAGGTGCT GTCCAAAGAC TCCAAAGTGT CTGGAGATGA GGGATGGTGG 
ACTGGAAAGA TCCAAGACAA GGTGGGCATA TTCCCTTGTA ATTATGTAAC CCAACGGGAT 
TCCAGCTATC CAGCGTTACC ACCGGAAGCT CTGGTTGGTG GAGATTCACC ACTGGAAATT 
GATTTCTTAG AGCTGCGATT GGAGGAAGTG ATTGGTGCTG GCGGCTTTGG AAAGGTGTAT 
AAGGGTGTAT GGCGAGCTGA GGAAGTGGCT GTCAAAGCAG CTCGTCAGGA CCCAGATGAG 
GACATCAGTG CCACGGCAGA GAATGTGAGG CAGGAGGCCA GACTCTTCTG GATGCTGCGT 
CACCGCAACA TCATCGCTCT ACGAGGTGTT TGTCTGCGGG AGCCAAATCT TTGCCTTGTT 
ATGGAATATG CCCGTGGTGG GGCGCTTAAC CGGGCACTTG CTGGCAAGAA GGTGCCTCCT 
CGGGTACTGG TGAATTGGGC TGTGCAGATT GCAACTGGAA TGGACTACTT GCACAACCAG 
ACCTTTGTGC CAATCATCCA TCGAGACCTC AAATCCAGCA ACATTCTGAT TCTGGAGCCT 
GTGGAGAGAG ATGACCTGAG TGGAAAGACA CTGAAGATCA CAGACTTTGG TCTTGCTCGA 
GAATGGCATC GCACCACCAA GATGAGCGCA GCGGGCACGT ATGCCTGGAT GGCTCCAGAG 
GTCATCAAGC TCTCCCTGTT CTCCAAAAGC AGCGATGTGT GGAGTTTTGG AGTGTTATTG 
TGGGAGCTGC TGACTGGTGA AGTGCCATAC CGTGAGATCG ACGCTTTGGC TGTGGCCTAT 
GGAGTTGCCA TGAACAAGTT GACTCTTCCC ATCCCCTCCA CCTGCCCTGA AGCCTTTGCC 
CAGCTGCTGG GAGAGTGTTG GTGTCCTAAC CCACGTGGAC GACCTGCTTT TGGGAGTATC 
CTGAAGAGGC TGGAGGACAT CGAGCAGTCG GCCATGTTCC AGATGCCCCT GGAGTCTTTC 
CATTCCCTGC AGGAGGACTG GAGGCTGGAG ATTCAGCAAA TGTTTGATGA ACTACGAGCA 
AAGGAGAAGG AGTTGCGATC CTGGGAGGAA GCTCTGGCCC GGGCAGCGGA GGAGCAGAGG 
GAGCAGGAGG AGCATCTGAG GAGACGCGAG CAGGAGTTAG CAGAGCGAGA GATCGACATA 
GTGGAGAGAG AGCTCAACAT CATCATCCAT CAGATGTACC AAGAGAAACC GCAGGTCAAA 
AAGCGCAAAG GCAACTTCAA AAAGAGCCGT CTGCTCAAGC TCGGCAGAGA CAGCAACTGC 
ATCAGTCTGC CATCTGGTTT TGAGCATAAG ATCACAGTGC AAGCTTCGCC CAGTGTGGAC 
AAGAGGAAAT GTCAGAGTAG TGAAAGCACC ACTCCTCCAG CCAGCCCAGG GGTCATTCCT 
CGCCTCAGAG CCTTTCGCTT GACACCCAGT GATGGGAGGA AGACGTGGGG CCGGACGGCA 
GTGTGTAAGA AAGAGGAGCC GGGGACCAAA AAGAAGGGCC GAACATGGGG TCCGAGCTCC 
ACACATCAGC GTGAGAGAGT CGGTGGAGAG GACAGGCTAA AGTCTCTTGG GGAGGGCAAA 
GTGTGGTCCT CCAGTGCTCC AAATCTGGGG AAGTCCCCAA AACATGCTCC TATGACAGCT 
GGATTCTCAA GCCTAAATGA AATGGAGGAG CACTGTGAGT TTGATGACAG TTCTCCAGGG 
CGCCTGGCTC CTGATCTGGG CAGTAACGGT GCGCTGGAAG ACGTGGGCTC GGGATCAGCA 
GGTTTAGGGT CAGGAATAGG CTCTCAGGAT TCAGTTTGCC GCTGCAGCCA GAGGAAGAAG 
AGTGACCTGC TGTTGCTGGG CTGTGCCTCT CTACTGGCCG TTGTGGGACT CGGTTCTGAC 
ATCCTCCAGT TGGGACGACA ACAGATGAAT CAAGATGAGC AGGACGTGCG AGAGGAGCAG 
CGCAAGAAAA AAGAGGGCCT GTTCCAGCGC ACGGGTCGAT TCAGACGCAG CACTTCTCCA 
CCCAGCCGCA CACTCTCCCT TTCCCTGTCC CACAACCGCC ATCACGACTC CATCTTGCCC 
AGCATGGACC CGTCTCCATC AGTCACCCTC CTGTCCCTGT CCTCCCTCTC TGACTGTAAC 
TCCACCAAAT CCCTGTTGCC TTCTGATCCT GATGACTTTG CCCCGACCCC TAGTAATGCC 
CCTCCGGTGG TGTCACCCGC ACCGGCATTA AACCCTCTGC TGGACCTGAG AGCGGAGAGC 
TTTAAAAGAG AGCCCAATCA GTCCCTCACT CCCACCCATG TCTCTGCAAC CATGGCATTA 
AACCGCGGGC ACCGGCGGAC GCCCTCTGAT GGAGCCATTC GTCCTCGAGC GCAGACGGTT 
GTGGGGCATC GCCGGACCCC TTCTGATGGG AGTATGCCCT TCCCGCCTGC TCATGCCCCT 
ACTATTACAC CATATACAGG TTCTCGAGAA ACTCTTGACA TTCCACGTCT GCCTGACCCG 
TCTATGATGT TCCCCTCAGC ACTTCGGCGC AAACCTGCAC CTCCCATCCC CACACCTGAG 
CCAGATAGCT ATACGGGCAC TCTAGAGCGT CCCAAAACCC TGGAGTTTGC TCCTCGTCCC 
CGTCCCACCC CAGCCCGTGC CCGTCCAGAC CCCTGGAAAC TGTCTTCTCT GTCCCAGACA 
CTCAGCTCTT CTCCGGGCAG CAGCTGCGAC AGTCCGCTGG GTTCGGGTGA CAGCGGTGTC 
AGTGCCGGCA CCGGCGGTGT CCATCAGAGC CTCCTGGACA TGGACATGGA GGGCCAGAGC 
CAGGACAGCA CGGTCCCCCT GTGCGGACAA CGCGCAAAGG CCACTCTCTG CGCTCAGCAT 
TCCTCATAG