SI:CH211-214P16.3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH211-214P16.3
Entrez Gene ID	560649
Full Name	si:ch211-214p16.3
General protein information	Preferred Names si:ch211-214p16.3 Names phospholipase B1, membrane-associated si:ch211-214p16.3
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017351817.2	XP_017207306.2	phospholipase B1, membrane-associated
XM_017351817.1	XP_017207306.1	phospholipase B1, membrane-associated

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PLB1	XP_005630350.1
Mus musculus (house mouse)	Plb1	NP_001074876.1
Rattus norvegicus (Norway rat)	Plb1	NP_620253.1
Homo sapiens (human)	PLB1	NP_694566.4
Gallus gallus (chicken)	PLB1	XP_004935430.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011512	XP_315274.4
Xenopus tropicalis (tropical clawed frog)	LOC100495859	XP_004914985.1
Pan troglodytes (chimpanzee)	PLB1	XP_515372.2
Xenopus tropicalis (tropical clawed frog)	LOC100492698	XP_002943699.2
Bos taurus (cattle)	PLB1	XP_002691542.2
Danio rerio (zebrafish)	si:ch211-214p16.3	XP_689138.6
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011513	XP_315272.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011511	XP_315275.3

Gene Ontology

The following SI:CH211-214P16.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH211-214P16.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa43324	XM_017351817.2 Latest version!	Danio rerio si:ch211-214p16.3 (si:ch211-214p16.3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa55725	XM_017351817.1 Latest version!	Danio rerio si:ch211-214p16.3 (si:ch211-214p16.3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH211-214P16.3 ( XM_017351817.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207306.2 Danio rerio si:ch211-214p16.3 (si:ch211-214p16.3), mRNA.

Price & Availability↑

CloneID	ODa43324
Clone ID Related Accession (Same CDS sequence)	XM_017351817.2
Accession Version	XM_017351817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phospholipase B1, membrane-associated
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCAA TAAAAGATAA AATGTTTTCC TCCTTTGCAC AGATTATCAT CCTGGCAGTT 
CTAGCAAGCT GGTGTTCAGC AGTTGTTCCT GGTCCTCTGT TTTGTGCAGA TTCACCCGCC 
TCACCCAGCA CTCCAGTTTC TGTCCATTCT CTGAGGCCAG CTGATGTATC TGTGGTATCA 
GCAATAGTTC TGTCTGAAGT GGAGAGATCT GATGAAATTA GAGCCCTGAG CATGCTGTCT 
GAAATATTGT CCACCTTTAA CCCTCATATA TCAGCTGTTC TACCTGACCA AAGGAGTTTA 
ATGGAAAAAG CAGAAGACAT GCAGTTCCTG GACCCCACTC AGTGGAAGTT GCTCCTTCTA 
TTCATACCAG TTGATGAGAT CTCTGTGTGC CCAGACCAGG ATCTCACTGC AGTCATTGAT 
GAAACAGTTA ACAGAGTTGA GCAAACACTG GACTCTCTTA ATGAGAAGTT GAAACACACA 
TTGGTTCATG TTGTGGTTTG GCGTGGACTG TCAAGTGACC GAGTCTGTCA GTTCAAATGT 
GAGCAAGGAT ATGATGAAAA CAAATGTAGA CTGGATATGT TAGTGCTTAT GGCTTCATTG 
CAGGAATCTC TCAGTGCTCT CTTAGAAAGG AGAGGCTGGT TCAACGATCA AGAGGACTTC 
AGTGTAATGC TTCAGTCCAG TCCAGTGCTG ACAGATCCAA TGACATTACC CTCGCCTGAC 
TCCTCAAATG ACATAAGCTT GGTTGTTTTA CAACTCTGGG CTAATTTGCT CCAGCCAATC 
ACAGACAACA CTGAGATGGA TGGGTGGGGC GGAGTCACCG TTCCATGTCC TACTGAGGAA 
AAGCCATTTT TGCGAACACA TCAAAATTCA GAGTCCAGAT CATCACAAAC AGCAGATTCA 
CCTCTACTTG TCGACCCAGT GATGGGGAGT GAACTCAACT GTGAGGACAT GGCTCCATCT 
CCCAGCATCC CTACCTCAGT TCATGCTTTA CGGCCAGCAG ACATCAAAGT TGTGGCAGCG 
ATAGGTGACT CTTTAACGGC AGCCAATGGG GTAGGTGCTG CTCAAAATAA CCTGCTGGGT 
GTGATCACAG AGTACCGAGG CCTGTCATGG TGCATTGGTG GAGATAAGAA CCTGTCATCT 
ATAACCACTT TACCAAACAT ACTGAAAGAG TTTAACCCTT CAATCAATGG CTATTCTGTG 
GGAAAAGGAG ATGAGGATTC AGCACAAAGC TTTCTGAACC AAGCAGTACC AGGAGCCAAA 
GCTGATGATA TGGTGAAGCA GGCTCATGCT GTTATTAAAA GAATGAAGGA AGACTCAAGA 
ATTGATTTCG AGAACGACTG GAAGATTATC ACACTCTTCA TTGGAGGAAA TGACTTATGT 
GCCCACTGCT CTGATACTCG GTACTATTCT TCTGATAACA TTGTGAAGTA TATCCGCGAG 
GCTCTGGACA TTTTGCACAA TGAGGTTCCT CGAGCTCTGG TAAACCTCGT GGAGCTGCTG 
GATATAGTTC CCCTTCGTGC ACTTCATAAG GACAAAAGCA ATGGTTGCCC TACCTGGCTG 
GTCAATATTA TGTGCCGTTG TGTTCTGCAA CCTAAAGATG AATCTCATGA ATTCCAGACA 
TTGCAGGAGT TTAACAAAGC CTATCAGCGT GGGATGTGGG AGCTGGTGGA TTCTGGCCGA 
TATGACACTC ATGATAATTT CACTGTGGTC CTGCAGCCAT TCTTGAGAAA TGTTTTTCTT 
CCCTTGATGG AGGACGGGAG ACCTGACCGA TCTTATTTCT CTCCGGACTG TTTTCATCTC 
AGTCAGAAAG CTCACACGCT CATGGCTCGG GCTCTCTGGA ACAACCTGAT GGAACCTTTA 
GGTAACAAGA CTGATAAAGA GGATTTCACT GCAGGAATCT CTCTTAAATG CCCAGAAAGA 
TCATCCCCCT ATTTGAGGAC ATTTCATAAC AGCAATTACA CATACAACAT TCCTGAACCT 
TCTCCTCCAC CCCCAACAAA CTGGGGAAGT GATTTCTCAT GTGTGGACAC AGCGCCCTCT 
AACACTTTAC CAACATCTGT TCACAGTCTG CGTCCAGGTG ACATCAAAGT AGTGGCGGCT 
CTTGGAGACT CACTCACTGC CGGATTTGGT GCAAAAGCTA AAAACATTCT CCAGCTGCCG 
GATGAGGAAC GCGGGGTTTC CTGGAGCATT GGTGGAGATG ATACATTGGA GACTGTCACC 
ACATTACCGA ACATCTTCAA GAAGTTCAAC CCAAGTGTTT TCGGCTTCTC CAAGGGGAAA 
AGTAAACGAC CAAATGGCTT TAATATGGCA GTGGGCGGTG CCAAAGCACA GAATATACCA 
GCACAAGCAA GGGATCTCAT CACAGCTTTG AAGGAAAGCA AGGACGTGGA CTTTGAGAAG 
GATTGGAAAC TGGTGACATT GTTTATTGGA GGAAATGACC TTTGCCAGTA CTGTCATGAC 
CGGGCTGCTC TGTCTTCTTC AAAGTATATC GGTCACATCA GAGACAGTCT GGACATGCTC 
TACAATGAGG TTCCCAGAGT GCTGGTGAAT TTAGTGGAAA TCTTACAGAT TGAGGATCTG 
CGAATGATAA AGAGGGACAC ACTGGGCTGC AGCCTCCTCC AAAAAAACAT GTGTCCGTGT 
TTTCTGAGTC CACGTGAAAA CTCTCCACAA ATGAATGAAA TGAAGAAGGT CAACAGAGAT 
CTGCAGGCGG AGGCTGAAAG GCTGGTGTAT GGGGGACGGT ATGATGGAAG ACATGACTTT 
GCGGTGGTGC TACAGCCCTT TTTTGAGAAC ACTGTTGTTC CAATGGACAA GGATGGCAAA 
CCAGACGTGT CTTTCTTTTC TGTGGATTGT TTTCATTTTT CTGAGCGTGG CCATGCAGAG 
ATGGCTATTG CAATTTGGAA CAATATGCTG GAGCCTGTAG GGAGTAAACA GAACTACAAT 
AATTTCACAT ATGACCGCAG TAAGATCCGC TGTCCCACAG AGGAGCATCC CTTCATCTTC 
ACACGAATAA ACAGTGGCCC CGAGGTTCCT ATGACAACAG TACCCACCAC AGTCCTACCG 
GGCACCCCTT TGCCTGCTGA TTGTGCCAAT GATGTTGTGC CAGCCTGGGC AGCTGCAGTT 
CTGGCTGTAG GAGGATTGAT GATTGGCTGG ATCGTAACCT GGATGATCTT CTACTATAGA 
GAACGACGAA ACAGAAAAAG AAACCAGATC AATGAAATGA GTGGAACAAA GTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207306.2
CDS	87..3323
Translation	MMAIKDKMFSSFAQIIILAVLASWCSAVVPGPLFCADSPASPSTPVSVHSLRPADVSVVSAIVLSEVERSDEIRALSMLSEILSTFNPHISAVLPDQRSLMEKAEDMQFLDPTQWKLLLLFIPVDEISVCPDQDLTAVIDETVNRVEQTLDSLNEKLKHTLVHVVVWRGLSSDRVCQFKCEQGYDENKCRLDMLVLMASLQESLSALLERRGWFNDQEDFSVMLQSSPVLTDPMTLPSPDSSNDISLVVLQLWANLLQPITDNTEMDGWGGVTVPCPTEEKPFLRTHQNSESRSSQTADSPLLVDPVMGSELNCEDMAPSPSIPTSVHALRPADIKVVAAIGDSLTAANGVGAAQNNLLGVITEYRGLSWCIGGDKNLSSITTLPNILKEFNPSINGYSVGKGDEDSAQSFLNQAVPGAKADDMVKQAHAVIKRMKEDSRIDFENDWKIITLFIGGNDLCAHCSDTRYYSSDNIVKYIREALDILHNEVPRALVNLVELLDIVPLRALHKDKSNGCPTWLVNIMCRCVLQPKDESHEFQTLQEFNKAYQRGMWELVDSGRYDTHDNFTVVLQPFLRNVFLPLMEDGRPDRSYFSPDCFHLSQKAHTLMARALWNNLMEPLGNKTDKEDFTAGISLKCPERSSPYLRTFHNSNYTYNIPEPSPPPPTNWGSDFSCVDTAPSNTLPTSVHSLRPGDIKVVAALGDSLTAGFGAKAKNILQLPDEERGVSWSIGGDDTLETVTTLPNIFKKFNPSVFGFSKGKSKRPNGFNMAVGGAKAQNIPAQARDLITALKESKDVDFEKDWKLVTLFIGGNDLCQYCHDRAALSSSKYIGHIRDSLDMLYNEVPRVLVNLVEILQIEDLRMIKRDTLGCSLLQKNMCPCFLSPRENSPQMNEMKKVNRDLQAEAERLVYGGRYDGRHDFAVVLQPFFENTVVPMDKDGKPDVSFFSVDCFHFSERGHAEMAIAIWNNMLEPVGSKQNYNNFTYDRSKIRCPTEEHPFIFTRINSGPEVPMTTVPTTVLPGTPLPADCANDVVPAWAAAVLAVGGLMIGWIVTWMIFYYRERRNRKRNQINEMSGTKF

Target ORF information:

RefSeq Version	XM_017351817.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-214p16.3 (si:ch211-214p16.3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351817.2

ORF Insert Sequence:

ATGATGGCAA TAAAAGATAA AATGTTTTCC TCCTTTGCAC AGATTATCAT CCTGGCAGTT 
CTAGCAAGCT GGTGTTCAGC AGTTGTTCCT GGTCCTCTGT TTTGTGCAGA TTCACCCGCC 
TCACCCAGCA CTCCAGTTTC TGTCCATTCT CTGAGGCCAG CTGATGTATC TGTGGTATCA 
GCAATAGTTC TGTCTGAAGT GGAGAGATCT GATGAAATTA GAGCCCTGAG CATGCTGTCT 
GAAATATTGT CCACCTTTAA CCCTCATATA TCAGCTGTTC TACCTGACCA AAGGAGTTTA 
ATGGAAAAAG CAGAAGACAT GCAGTTCCTG GACCCCACTC AGTGGAAGTT GCTCCTTCTA 
TTCATACCAG TTGATGAGAT CTCTGTGTGC CCAGACCAGG ATCTCACTGC AGTCATTGAT 
GAAACAGTTA ACAGAGTTGA GCAAACACTG GACTCTCTTA ATGAGAAGTT GAAACACACA 
TTGGTTCATG TTGTGGTTTG GCGTGGACTG TCAAGTGACC GAGTCTGTCA GTTCAAATGT 
GAGCAAGGAT ATGATGAAAA CAAATGTAGA CTGGATATGT TAGTGCTTAT GGCTTCATTG 
CAGGAATCTC TCAGTGCTCT CTTAGAAAGG AGAGGCTGGT TCAACGATCA AGAGGACTTC 
AGTGTAATGC TTCAGTCCAG TCCAGTGCTG ACAGATCCAA TGACATTACC CTCGCCTGAC 
TCCTCAAATG ACATAAGCTT GGTTGTTTTA CAACTCTGGG CTAATTTGCT CCAGCCAATC 
ACAGACAACA CTGAGATGGA TGGGTGGGGC GGAGTCACCG TTCCATGTCC TACTGAGGAA 
AAGCCATTTT TGCGAACACA TCAAAATTCA GAGTCCAGAT CATCACAAAC AGCAGATTCA 
CCTCTACTTG TCGACCCAGT GATGGGGAGT GAACTCAACT GTGAGGACAT GGCTCCATCT 
CCCAGCATCC CTACCTCAGT TCATGCTTTA CGGCCAGCAG ACATCAAAGT TGTGGCAGCG 
ATAGGTGACT CTTTAACGGC AGCCAATGGG GTAGGTGCTG CTCAAAATAA CCTGCTGGGT 
GTGATCACAG AGTACCGAGG CCTGTCATGG TGCATTGGTG GAGATAAGAA CCTGTCATCT 
ATAACCACTT TACCAAACAT ACTGAAAGAG TTTAACCCTT CAATCAATGG CTATTCTGTG 
GGAAAAGGAG ATGAGGATTC AGCACAAAGC TTTCTGAACC AAGCAGTACC AGGAGCCAAA 
GCTGATGATA TGGTGAAGCA GGCTCATGCT GTTATTAAAA GAATGAAGGA AGACTCAAGA 
ATTGATTTCG AGAACGACTG GAAGATTATC ACACTCTTCA TTGGAGGAAA TGACTTATGT 
GCCCACTGCT CTGATACTCG GTACTATTCT TCTGATAACA TTGTGAAGTA TATCCGCGAG 
GCTCTGGACA TTTTGCACAA TGAGGTTCCT CGAGCTCTGG TAAACCTCGT GGAGCTGCTG 
GATATAGTTC CCCTTCGTGC ACTTCATAAG GACAAAAGCA ATGGTTGCCC TACCTGGCTG 
GTCAATATTA TGTGCCGTTG TGTTCTGCAA CCTAAAGATG AATCTCATGA ATTCCAGACA 
TTGCAGGAGT TTAACAAAGC CTATCAGCGT GGGATGTGGG AGCTGGTGGA TTCTGGCCGA 
TATGACACTC ATGATAATTT CACTGTGGTC CTGCAGCCAT TCTTGAGAAA TGTTTTTCTT 
CCCTTGATGG AGGACGGGAG ACCTGACCGA TCTTATTTCT CTCCGGACTG TTTTCATCTC 
AGTCAGAAAG CTCACACGCT CATGGCTCGG GCTCTCTGGA ACAACCTGAT GGAACCTTTA 
GGTAACAAGA CTGATAAAGA GGATTTCACT GCAGGAATCT CTCTTAAATG CCCAGAAAGA 
TCATCCCCCT ATTTGAGGAC ATTTCATAAC AGCAATTACA CATACAACAT TCCTGAACCT 
TCTCCTCCAC CCCCAACAAA CTGGGGAAGT GATTTCTCAT GTGTGGACAC AGCGCCCTCT 
AACACTTTAC CAACATCTGT TCACAGTCTG CGTCCAGGTG ACATCAAAGT AGTGGCGGCT 
CTTGGAGACT CACTCACTGC CGGATTTGGT GCAAAAGCTA AAAACATTCT CCAGCTGCCG 
GATGAGGAAC GCGGGGTTTC CTGGAGCATT GGTGGAGATG ATACATTGGA GACTGTCACC 
ACATTACCGA ACATCTTCAA GAAGTTCAAC CCAAGTGTTT TCGGCTTCTC CAAGGGGAAA 
AGTAAACGAC CAAATGGCTT TAATATGGCA GTGGGCGGTG CCAAAGCACA GAATATACCA 
GCACAAGCAA GGGATCTCAT CACAGCTTTG AAGGAAAGCA AGGACGTGGA CTTTGAGAAG 
GATTGGAAAC TGGTGACATT GTTTATTGGA GGAAATGACC TTTGCCAGTA CTGTCATGAC 
CGGGCTGCTC TGTCTTCTTC AAAGTATATC GGTCACATCA GAGACAGTCT GGACATGCTC 
TACAATGAGG TTCCCAGAGT GCTGGTGAAT TTAGTGGAAA TCTTACAGAT TGAGGATCTG 
CGAATGATAA AGAGGGACAC ACTGGGCTGC AGCCTCCTCC AAAAAAACAT GTGTCCGTGT 
TTTCTGAGTC CACGTGAAAA CTCTCCACAA ATGAATGAAA TGAAGAAGGT CAACAGAGAT 
CTGCAGGCGG AGGCTGAAAG GCTGGTGTAT GGGGGACGGT ATGATGGAAG ACATGACTTT 
GCGGTGGTGC TACAGCCCTT TTTTGAGAAC ACTGTTGTTC CAATGGACAA GGATGGCAAA 
CCAGACGTGT CTTTCTTTTC TGTGGATTGT TTTCATTTTT CTGAGCGTGG CCATGCAGAG 
ATGGCTATTG CAATTTGGAA CAATATGCTG GAGCCTGTAG GGAGTAAACA GAACTACAAT 
AATTTCACAT ATGACCGCAG TAAGATCCGC TGTCCCACAG AGGAGCATCC CTTCATCTTC 
ACACGAATAA ACAGTGGCCC CGAGGTTCCT ATGACAACAG TACCCACCAC AGTCCTACCG 
GGCACCCCTT TGCCTGCTGA TTGTGCCAAT GATGTTGTGC CAGCCTGGGC AGCTGCAGTT 
CTGGCTGTAG GAGGATTGAT GATTGGCTGG ATCGTAACCT GGATGATCTT CTACTATAGA 
GAACGACGAA ACAGAAAAAG AAACCAGATC AATGAAATGA GTGGAACAAA GTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-214P16.3 ( XM_017351817.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207306.1 Danio rerio si:ch211-214p16.3 (si:ch211-214p16.3), mRNA.

Price & Availability↑

CloneID	ODa55725
Clone ID Related Accession (Same CDS sequence)	XM_017351817.1
Accession Version	XM_017351817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phospholipase B1, membrane-associated
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCTG AAATATTGTC CACCTTTAAC CCTCATATAT CAGCTGTTCT ACCTGACCAA 
AGGAGTTTAA TGGAAAAAGC AGAAGACATG CAGTTCCTGG ACCCCACTCA GTGGAAGTTG 
CTCCTTCTAT TCATACCAGT TGATGAGATC TCTGTGTGCC CAGACCAGGA TCTCACTGCA 
GTCATTGATG AAACAGTTAA CAGAGTTGAG CAAACACTGG ACTCTCTTAA TGAGAAGTTG 
AAACACACAT TGGTTCATGT TGTGGTTTGG CGTGGACTGT CAAGTGACCG AGTCTGTCAG 
TTCAAATGTG AGCAAGGATA TGATGAAAAC AAATGTAGAC TGGATATGTT AGTGCTTATG 
GCTTCATTGC AGGAATCTCT CAGTGCTCTC TTAGAAAGGA GAGGCTGGTT CAACGATCAA 
GAGGACTTCA GTGTAATGCT TCAGTCCAGT CCAGTGCTGA CAGATCCAAT GACATTACCC 
TCGCCTGACT CCTCAAATGA CATAAGCTTG GTTGTTTTAC AACTCTGGGC TAATTTGCTC 
CAGCCAATCA CAGACAACAC TGAGATGGAT GGGTGGGGCG GAGTCACCGT TCCATGTCCT 
ACTGAGGAAA AGCCATTTTT GCGAACACAT CAAAATTCAG AGTCCAGATC ATCACAAACA 
GCAGATTCAC CTCTACTTGT CGACCCAGTG ATGGGGAGTG AACTCAACTG TGAGGACATG 
GCTCCATCTC CCAGCATCCC TACCTCAGTT CATGCTTTAC GGCCAGCAGA CATCAAAGTT 
GTGGCAGCGA TAGGTGACTC TTTAACGGCA GCCAATGGGG TAGGTGCTGC TCAAAATAAC 
CTGCTGGGTG TGATCACAGA GTACCGAGGC CTGTCATGGT GCATTGGTGG AGATAAGAAC 
CTGTCATCTA TAACCACTTT ACCAAACATA CTGAAAGAGT TTAACCCTTC AATCAATGGC 
TATTCTGTGG GAAAAGGAGA TGAGGATTCA GCACAAAGCT TTCTGAACCA AGCAGTACCA 
GGAGCCAAAG CTGATGATAT GGTGAAGCAG GCTCATGCTG TTATTAAAAG AATGAAGGAA 
GACTCAAGAA TTGATTTCGA GAACGACTGG AAGATTATCA CACTCTTCAT TGGAGGAAAT 
GACTTATGTG CCCACTGCTC TGATACTCGG TACTATTCTT CTGATAACAT TGTGAAGTAT 
ATCCGCGAGG CTCTGGACAT TTTGCACAAT GAGGTTCCTC GAGCTCTGGT AAACCTCGTG 
GAGCTGCTGG ATATAGTTCC CCTTCGTGCA CTTCATAAGG ACAAAAGCAA TGGTTGCCCT 
ACCTGGCTGG TCAATATTAT GTGCCGTTGT GTTCTGCAAC CTAAAGATGA ATCTCATGAA 
TTCCAGACAT TGCAGGAGTT TAACAAAGCC TATCAGCGTG GGATGTGGGA GCTGGTGGAT 
TCTGGCCGAT ATGACACTCA TGATAATTTC ACTGTGGTCC TGCAGCCATT CTTGAGAAAT 
GTTTTTCTTC CCTTGATGGA GGACGGGAGA CCTGACCGAT CTTATTTCTC TCCGGACTGT 
TTTCATCTCA GTCAGAAAGC TCACACGCTC ATGGCTCGGG CTCTCTGGAA CAACCTGATG 
GAACCTTTAG GTAACAAGAC TGATAAAGAG GATTTCACTG CAGGAATCTC TCTTAAATGC 
CCAGAAAGAT CATCCCCCTA TTTGAGGACA TTTCATAACA GCAATTACAC ATACAACATT 
CCTGAACCTT CTCCTCCACC CCCAACAAAC TGGGGAAGTG ATTTCTCATG TGTGGACACA 
GCGCCCTCTA ACACTTTACC AACATCTGTT CACAGTCTGC GTCCAGGTGA CATCAAAGTA 
GTGGCGGCTC TTGGAGACTC ACTCACTGCC GGATTTGGTG CAAAAGCTAA AAACATTCTC 
CAGCTGCCGG ATGAGGAACG CGGGGTTTCC TGGAGCATTG GTGGAGATGA TACATTGGAG 
ACTGTCACCA CATTACCGAA CATCTTCAAG AAGTTCAACC CAAGTGTTTT CGGCTTCTCC 
AAGGGGAAAA GTAAACGACC AAATGGCTTT AATATGGCAG TGGGCGGTGC CAAAGCACAG 
AATATACCAG CACAAGCAAG GGATCTCATC ACAGCTTTGA AGGAAAGCAA GGACGTGGAC 
TTTGAGAAGG ATTGGAAACT GGTGACATTG TTTATTGGAG GAAATGACCT TTGCCAGTAC 
TGTCATGACC GGGCTGCTCT GTCTTCTTCA AAGTATATCG GTCACATCAG AGACAGTCTG 
GACATGCTCT ACAATGAGGT TCCCAGAGTG CTGGTGAATT TAGTGGAAAT CTTACAGATT 
GAGGATCTGC GAATGATAAA GAGGGACACA CTGGGCTGCA GCCTCCTCCA AAAAAACATG 
TGTCCGTGTT TTCTGAGTCC ACGTGAAAAC TCTCCACAAA TGAATGAAAT GAAGAAGGTC 
AACAGAGATC TGCAGGCGGA GGCTGAAAGG CTGGTGTATG GGGGACGGTA TGATGGAAGA 
CATGACTTTG CGGTGGTGCT ACAGCCCTTT TTTGAGAACA CTGTTGTTCC AATGGACAAG 
GATGGCAAAC CAGACGTGTC TTTCTTTTCT GTGGATTGTT TTCATTTTTC TGAGCGTGGC 
CATGCAGAGA TGGCTATTGC AATTTGGAAC AATATGCTGG AGCCTGTAGG GAGTAAACAG 
AACTACAATA ATTTCACATA TGACCGCAGT AAGATCCGCT GTCCCACAGA GGAGCATCCC 
TTCATCTTCA CACGAATAAA CAGTGGCCCC GAGGTTCCTA TGACAACAGT ACCCACCACA 
GTCCTACCGG GCACCCCTTT GCCTGCTGAT TGTGCCAATG ATGTTGTGCC AGCCTGGGCA 
GCTGCAGTTC TGGCTGTAGG AGGATTGATG ATTGGCTGGA TCGTAACCTG GATGATCTTC 
TACTATAGAG AACGACGAAA CAGAAAAAGA AACCAGATCA ATGAAATGAG TGGAACAAAG 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207306.1
CDS	26..3031
Translation	MLSEILSTFNPHISAVLPDQRSLMEKAEDMQFLDPTQWKLLLLFIPVDEISVCPDQDLTAVIDETVNRVEQTLDSLNEKLKHTLVHVVVWRGLSSDRVCQFKCEQGYDENKCRLDMLVLMASLQESLSALLERRGWFNDQEDFSVMLQSSPVLTDPMTLPSPDSSNDISLVVLQLWANLLQPITDNTEMDGWGGVTVPCPTEEKPFLRTHQNSESRSSQTADSPLLVDPVMGSELNCEDMAPSPSIPTSVHALRPADIKVVAAIGDSLTAANGVGAAQNNLLGVITEYRGLSWCIGGDKNLSSITTLPNILKEFNPSINGYSVGKGDEDSAQSFLNQAVPGAKADDMVKQAHAVIKRMKEDSRIDFENDWKIITLFIGGNDLCAHCSDTRYYSSDNIVKYIREALDILHNEVPRALVNLVELLDIVPLRALHKDKSNGCPTWLVNIMCRCVLQPKDESHEFQTLQEFNKAYQRGMWELVDSGRYDTHDNFTVVLQPFLRNVFLPLMEDGRPDRSYFSPDCFHLSQKAHTLMARALWNNLMEPLGNKTDKEDFTAGISLKCPERSSPYLRTFHNSNYTYNIPEPSPPPPTNWGSDFSCVDTAPSNTLPTSVHSLRPGDIKVVAALGDSLTAGFGAKAKNILQLPDEERGVSWSIGGDDTLETVTTLPNIFKKFNPSVFGFSKGKSKRPNGFNMAVGGAKAQNIPAQARDLITALKESKDVDFEKDWKLVTLFIGGNDLCQYCHDRAALSSSKYIGHIRDSLDMLYNEVPRVLVNLVEILQIEDLRMIKRDTLGCSLLQKNMCPCFLSPRENSPQMNEMKKVNRDLQAEAERLVYGGRYDGRHDFAVVLQPFFENTVVPMDKDGKPDVSFFSVDCFHFSERGHAEMAIAIWNNMLEPVGSKQNYNNFTYDRSKIRCPTEEHPFIFTRINSGPEVPMTTVPTTVLPGTPLPADCANDVVPAWAAAVLAVGGLMIGWIVTWMIFYYRERRNRKRNQINEMSGTKF

Target ORF information:

RefSeq Version	XM_017351817.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-214p16.3 (si:ch211-214p16.3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351817.1

ORF Insert Sequence:

ATGCTGTCTG AAATATTGTC CACCTTTAAC CCTCATATAT CAGCTGTTCT ACCTGACCAA 
AGGAGTTTAA TGGAAAAAGC AGAAGACATG CAGTTCCTGG ACCCCACTCA GTGGAAGTTG 
CTCCTTCTAT TCATACCAGT TGATGAGATC TCTGTGTGCC CAGACCAGGA TCTCACTGCA 
GTCATTGATG AAACAGTTAA CAGAGTTGAG CAAACACTGG ACTCTCTTAA TGAGAAGTTG 
AAACACACAT TGGTTCATGT TGTGGTTTGG CGTGGACTGT CAAGTGACCG AGTCTGTCAG 
TTCAAATGTG AGCAAGGATA TGATGAAAAC AAATGTAGAC TGGATATGTT AGTGCTTATG 
GCTTCATTGC AGGAATCTCT CAGTGCTCTC TTAGAAAGGA GAGGCTGGTT CAACGATCAA 
GAGGACTTCA GTGTAATGCT TCAGTCCAGT CCAGTGCTGA CAGATCCAAT GACATTACCC 
TCGCCTGACT CCTCAAATGA CATAAGCTTG GTTGTTTTAC AACTCTGGGC TAATTTGCTC 
CAGCCAATCA CAGACAACAC TGAGATGGAT GGGTGGGGCG GAGTCACCGT TCCATGTCCT 
ACTGAGGAAA AGCCATTTTT GCGAACACAT CAAAATTCAG AGTCCAGATC ATCACAAACA 
GCAGATTCAC CTCTACTTGT CGACCCAGTG ATGGGGAGTG AACTCAACTG TGAGGACATG 
GCTCCATCTC CCAGCATCCC TACCTCAGTT CATGCTTTAC GGCCAGCAGA CATCAAAGTT 
GTGGCAGCGA TAGGTGACTC TTTAACGGCA GCCAATGGGG TAGGTGCTGC TCAAAATAAC 
CTGCTGGGTG TGATCACAGA GTACCGAGGC CTGTCATGGT GCATTGGTGG AGATAAGAAC 
CTGTCATCTA TAACCACTTT ACCAAACATA CTGAAAGAGT TTAACCCTTC AATCAATGGC 
TATTCTGTGG GAAAAGGAGA TGAGGATTCA GCACAAAGCT TTCTGAACCA AGCAGTACCA 
GGAGCCAAAG CTGATGATAT GGTGAAGCAG GCTCATGCTG TTATTAAAAG AATGAAGGAA 
GACTCAAGAA TTGATTTCGA GAACGACTGG AAGATTATCA CACTCTTCAT TGGAGGAAAT 
GACTTATGTG CCCACTGCTC TGATACTCGG TACTATTCTT CTGATAACAT TGTGAAGTAT 
ATCCGCGAGG CTCTGGACAT TTTGCACAAT GAGGTTCCTC GAGCTCTGGT AAACCTCGTG 
GAGCTGCTGG ATATAGTTCC CCTTCGTGCA CTTCATAAGG ACAAAAGCAA TGGTTGCCCT 
ACCTGGCTGG TCAATATTAT GTGCCGTTGT GTTCTGCAAC CTAAAGATGA ATCTCATGAA 
TTCCAGACAT TGCAGGAGTT TAACAAAGCC TATCAGCGTG GGATGTGGGA GCTGGTGGAT 
TCTGGCCGAT ATGACACTCA TGATAATTTC ACTGTGGTCC TGCAGCCATT CTTGAGAAAT 
GTTTTTCTTC CCTTGATGGA GGACGGGAGA CCTGACCGAT CTTATTTCTC TCCGGACTGT 
TTTCATCTCA GTCAGAAAGC TCACACGCTC ATGGCTCGGG CTCTCTGGAA CAACCTGATG 
GAACCTTTAG GTAACAAGAC TGATAAAGAG GATTTCACTG CAGGAATCTC TCTTAAATGC 
CCAGAAAGAT CATCCCCCTA TTTGAGGACA TTTCATAACA GCAATTACAC ATACAACATT 
CCTGAACCTT CTCCTCCACC CCCAACAAAC TGGGGAAGTG ATTTCTCATG TGTGGACACA 
GCGCCCTCTA ACACTTTACC AACATCTGTT CACAGTCTGC GTCCAGGTGA CATCAAAGTA 
GTGGCGGCTC TTGGAGACTC ACTCACTGCC GGATTTGGTG CAAAAGCTAA AAACATTCTC 
CAGCTGCCGG ATGAGGAACG CGGGGTTTCC TGGAGCATTG GTGGAGATGA TACATTGGAG 
ACTGTCACCA CATTACCGAA CATCTTCAAG AAGTTCAACC CAAGTGTTTT CGGCTTCTCC 
AAGGGGAAAA GTAAACGACC AAATGGCTTT AATATGGCAG TGGGCGGTGC CAAAGCACAG 
AATATACCAG CACAAGCAAG GGATCTCATC ACAGCTTTGA AGGAAAGCAA GGACGTGGAC 
TTTGAGAAGG ATTGGAAACT GGTGACATTG TTTATTGGAG GAAATGACCT TTGCCAGTAC 
TGTCATGACC GGGCTGCTCT GTCTTCTTCA AAGTATATCG GTCACATCAG AGACAGTCTG 
GACATGCTCT ACAATGAGGT TCCCAGAGTG CTGGTGAATT TAGTGGAAAT CTTACAGATT 
GAGGATCTGC GAATGATAAA GAGGGACACA CTGGGCTGCA GCCTCCTCCA AAAAAACATG 
TGTCCGTGTT TTCTGAGTCC ACGTGAAAAC TCTCCACAAA TGAATGAAAT GAAGAAGGTC 
AACAGAGATC TGCAGGCGGA GGCTGAAAGG CTGGTGTATG GGGGACGGTA TGATGGAAGA 
CATGACTTTG CGGTGGTGCT ACAGCCCTTT TTTGAGAACA CTGTTGTTCC AATGGACAAG 
GATGGCAAAC CAGACGTGTC TTTCTTTTCT GTGGATTGTT TTCATTTTTC TGAGCGTGGC 
CATGCAGAGA TGGCTATTGC AATTTGGAAC AATATGCTGG AGCCTGTAGG GAGTAAACAG 
AACTACAATA ATTTCACATA TGACCGCAGT AAGATCCGCT GTCCCACAGA GGAGCATCCC 
TTCATCTTCA CACGAATAAA CAGTGGCCCC GAGGTTCCTA TGACAACAGT ACCCACCACA 
GTCCTACCGG GCACCCCTTT GCCTGCTGAT TGTGCCAATG ATGTTGTGCC AGCCTGGGCA 
GCTGCAGTTC TGGCTGTAGG AGGATTGATG ATTGGCTGGA TCGTAACCTG GATGATCTTC 
TACTATAGAG AACGACGAAA CAGAAAAAGA AACCAGATCA ATGAAATGAG TGGAACAAAG 
TTCTAA