gem cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gem
Entrez Gene ID	560566
Full Name	GTP binding protein overexpressed in skeletal muscle
Synonyms	zgc:153003
General protein information	Preferred Names GTP binding protein overexpressed in skeletal muscle Names GTP-binding protein GEM GTP binding protein gene overexpressed in skeletal muscle
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045849.1	NP_001039314.1	GTP-binding protein GEM

Pathways from BioSystems

Homologies

	GEM	XP_001087974.1
Danio rerio (zebrafish)	gem	NP_001039314.1
Rattus norvegicus (Norway rat)	Gem	NP_001100107.1
Xenopus tropicalis (tropical clawed frog)	gem	NP_001090822.1
Homo sapiens (human)	GEM	NP_859053.1
Gallus gallus (chicken)	GEM	NP_998744.1
Pan troglodytes (chimpanzee)	GEM	XP_001142383.1
Canis lupus familiaris (dog)	GEM	XP_854414.2
Bos taurus (cattle)	GEM	NP_001263234.1
Mus musculus (house mouse)	Gem	NP_034406.2

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

Ancient origins of RGK protein function: modulation of voltage-gated calcium channels preceded the protostome and deuterostome split.
Puhl HL 3rd, Lu VB, Won YJ, Sasson Y, Hirsch JA, Ono F, Ikeda SR
PloS one9(7)e100694(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gem gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gem cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa30556	NM_001045849.1 Latest version!	Danio rerio GTP binding protein overexpressed in skeletal muscle (gem), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gem ( NM_001045849.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001039314.1 Danio rerio GTP binding protein overexpressed in skeletal muscle (gem), mRNA.

Price & Availability↑

CloneID	ODa30556
Clone ID Related Accession (Same CDS sequence)	NM_001045849.1
Accession Version	NM_001045849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	GTP-binding protein GEM
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC122113.1. On Sep 6, 2006 this sequence version replaced XM_683964.1. ##Evidence-Data-START## Transcript exon combination :: BC122113.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACCCTGC TGGCGAGCGT GCGACGCCAC AGTATCCGCG TACAGCATCA CCTGCACCGG 
TGGAGCATCT GCGGAACGGA CGGAGGGCAG CTGCTCAACG ATGCTTTCCC GCGCTCCGAC 
ATCAGTATGT CCCGGTCCAG TTCCTGCTCC TCCGCGTCCT CAGATTCCGC TCTCTCCACA 
GAGTCTGCGA CGCCGGCATC AGTTGGACCC TTTACTGTAG TTCTGCTCGG GGACAACGGC 
GTCGGGAAGT CCGCTCTCGC TAGTATATTC GCCGGAGCAT CGGACAGCAT GGGCAGCGAG 
TGCGAGTTAT ATGGAGGTGA AATATTTGAG CAGACAATAA CAGTAGATGG CGAGAGGGCC 
TCCGTCACCC TACTTGACAC ATGGGATTCA CAGGATGAAG GCAGCTGGAC ACAGCAGCGA 
TGTTTGCAGA CAGGAGATGC CTTCATTATA GTTTATGCCA TAACAGATCG TTCAAGCTTC 
CTTCGTGCAT CGGACCTTCG TGTTCAGCTG CGGCGTGAGC GTGAGGTCGA CCGTACTCCC 
ATCATCCTGG TGGGCAATAA ATGTGACCTG GTCCGCTGTC GAGAAGTGTC CATTAGTGAG 
GGTCGTTCGT CAGCGGCTGT CTTTGACTGT AAGTTCATCG AGACGTCCGC AGCAATGCAA 
CACAACGTCT GGCCGCTGTT TGAGGGCATC ATCCGCCAGC TCCGTCTGCG AAGAGACAGC 
ATGGAGACCC TCAGCAGCCA TTCATCTCTC CAAAAACGCA GGGAAAGTCT CCCAAAGAAA 
GCCAAGAGAT TCATTAACAG AATGGTTGCT AAGAAGAACA AGCAGGCGGC TTTTAAACTC 
AAGTCTAAAT CATGTCATGA CCTGATGAGT CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039314.1
CDS	260..1135
Translation	MTLLASVRRHSIRVQHHLHRWSICGTDGGQLLNDAFPRSDISMSRSSSCSSASSDSALSTESATPASVGPFTVVLLGDNGVGKSALASIFAGASDSMGSECELYGGEIFEQTITVDGERASVTLLDTWDSQDEGSWTQQRCLQTGDAFIIVYAITDRSSFLRASDLRVQLRREREVDRTPIILVGNKCDLVRCREVSISEGRSSAAVFDCKFIETSAAMQHNVWPLFEGIIRQLRLRRDSMETLSSHSSLQKRRESLPKKAKRFINRMVAKKNKQAAFKLKSKSCHDLMSL

Target ORF information:

RefSeq Version	NM_001045849.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GTP binding protein overexpressed in skeletal muscle (gem), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045849.1

ORF Insert Sequence:

ATGACCCTGC TGGCGAGCGT GCGACGCCAC AGTATCCGCG TACAGCATCA CCTGCACCGG 
TGGAGCATCT GCGGAACGGA CGGAGGGCAG CTGCTCAACG ATGCTTTCCC GCGCTCCGAC 
ATCAGTATGT CCCGGTCCAG TTCCTGCTCC TCCGCGTCCT CAGATTCCGC TCTCTCCACA 
GAGTCTGCGA CGCCGGCATC AGTTGGACCC TTTACTGTAG TTCTGCTCGG GGACAACGGC 
GTCGGGAAGT CCGCTCTCGC TAGTATATTC GCCGGAGCAT CGGACAGCAT GGGCAGCGAG 
TGCGAGTTAT ATGGAGGTGA AATATTTGAG CAGACAATAA CAGTAGATGG CGAGAGGGCC 
TCCGTCACCC TACTTGACAC ATGGGATTCA CAGGATGAAG GCAGCTGGAC ACAGCAGCGA 
TGTTTGCAGA CAGGAGATGC CTTCATTATA GTTTATGCCA TAACAGATCG TTCAAGCTTC 
CTTCGTGCAT CGGACCTTCG TGTTCAGCTG CGGCGTGAGC GTGAGGTCGA CCGTACTCCC 
ATCATCCTGG TGGGCAATAA ATGTGACCTG GTCCGCTGTC GAGAAGTGTC CATTAGTGAG 
GGTCGTTCGT CAGCGGCTGT CTTTGACTGT AAGTTCATCG AGACGTCCGC AGCAATGCAA 
CACAACGTCT GGCCGCTGTT TGAGGGCATC ATCCGCCAGC TCCGTCTGCG AAGAGACAGC 
ATGGAGACCC TCAGCAGCCA TTCATCTCTC CAAAAACGCA GGGAAAGTCT CCCAAAGAAA 
GCCAAGAGAT TCATTAACAG AATGGTTGCT AAGAAGAACA AGCAGGCGGC TTTTAAACTC 
AAGTCTAAAT CATGTCATGA CCTGATGAGT CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

Ancient origins of RGK protein function: modulation of voltage-gated calcium channels preceded the protostome and deuterostome split.
PloS one9(7)e100694(2014)
Puhl HL 3rd,Lu VB,Won YJ,Sasson Y,Hirsch JA,Ono F,Ikeda SR