gpc5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gpc5a
Entrez Gene ID	559454
Full Name	glypican 5a
Synonyms	gpc5
General protein information	Preferred Names glypican 5a Names glypican-5 glypican 5 zmp:0000001300
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001302236.1	NP_001289165.1	glypican-5 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GPC5	XP_542637.4
Mus musculus (house mouse)	Gpc5	NP_780709.1
Rattus norvegicus (Norway rat)	Gpc5	NP_001100755.2
Drosophila melanogaster (fruit fly)	dally	NP_001246685.1
Homo sapiens (human)	GPC5	NP_004457.1
	GPC5	XP_002800848.1
Gallus gallus (chicken)	GPC5	XP_004938641.1
Danio rerio (zebrafish)	LOC559454	XP_001920549.4
Bos taurus (cattle)	GPC5	NP_001095540.1
Pan troglodytes (chimpanzee)	GPC5	XP_509696.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005799	XP_003435729.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Identification and expression analysis of zebrafish glypicans during embryonic development.
Gupta M, Brand M
PloS one8(11)e80824(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gpc5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpc5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa43099	NM_001302236.1 Latest version!	Danio rerio glypican 5a (gpc5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gpc5a ( NM_001302236.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001289165.1 Danio rerio glypican 5a (gpc5a), mRNA.

Price & Availability↑

CloneID	ODa43099
Clone ID Related Accession (Same CDS sequence)	NM_001302236.1
Accession Version	NM_001302236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	glypican-5 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KC791424.1. ##Evidence-Data-START## Transcript exon combination :: KC791424.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAAATGG CGATCCGTGG AATTTTGTTT TATACATTTT TTGCGGTTTT CACAGATGTC 
TGTCAGGGGA ATTTAATGGA CTGCGAAGAA GTGCGTAAAG TTTTTCAGTT GAAACAAATA 
GGTCCTGCAA AATCAATACC TGACACGCCG AGAACAGGTT CAGACCTGCA GGTTTGCTTA 
TCCAGGAATC TAACATGCTG CACCAAAAAG ATGGAGGAGC GTTACCAGGT GGCGGCCCGG 
CGGGATATTC AGAACCTTCT CCAGATCTCT AGCTCCAGCC TAAAGTTCCT CTTCTCACGA 
AATGTGGCCG CTTTCCAAGA AAGCGTGGAG TCTCTGGTGC GTCAGGCGGA GAACCACACC 
CTCTCCATCC TCCATCAGTC CCACCGTGAT CTCTCCAGCC CAGCAGCATC AGCACCAGTC 
GGGGAGTTTT TCACAGAAGT GGCTCTGTTC ATCCTGGGCT CTGAGCTCAG TCTAGAAGAG 
TCCTCTCAGA GCTTCTTCAA CGCTCTTTTC CCCTTAATAT ACGAGCGTCT GATTGACTCC 
CGTCTGTCTC CTCCATACGC CGAATGCCTG CGCTCGTCCG CTCAAGATCT AGCACCGTTC 
GGAGCAGCCC CAATCCGCCT GGCGTCCCAA ATCGCCCATG CTTCTCTACC TGCTCGCGTG 
TTTCTCCAAG CGCTCCATTT GGGCATCGAG ATCATAAATA CGACCGATCA CATCCAGCTG 
AGCCGCGAGT GCAGACGTGG CCTGCTGAGG ATGAGCTACT GCCCGCACTG CCAGGCTCTG 
ACCTCAAGCA AACCCTGCAT GGGATACTGC CTGAACGTGG TGCGCGGGTG TCTGGCTAAC 
CTGGCTGAAG TGGATGCCCA CTGGAGGGAG TTTGTACGCT CGCTGGAGAC GCTGGCAGGA 
CGGATGCACG GAGAGCTGGA TCTGGAGCAG GTGCTGCTGG GGGCGCATGA GCTGGTGAGG 
GACGCTGTGG CGCACGCTCA GAGGAGCACA GCGAGGATAC ATGCGCAGGT GCAACGTGTG 
TGTGGTCAGT CCAGCAGATG GACGGAGCCG GTGAACAGCG TCCAGCACAG CACAGGACGA 
GCATCAGACT CGATACCACT GAGAGGAGCA AACAGAAACA CAGAGGACTC GCTCAGCAGC 
CGCAGAACTG AGTTCCTCCA CAGTCTGCGT TTATATCGCA CTTTTTACGG CGCTCTGGCT 
GATCAGCTGT GCATCAGTGA ACTGGCCTCT GCAGACGGAC GGGCCTGCTG GAACGGAGAC 
GACATCGCCA GAAGCTATAC TCTGCGAGTG GTTGGCAATG GGATTAAAGC GCAGGCTGAA 
AACCCAGAAG TCAAAGTGAA GGAGGCCGAT CCGGTGATCA ATCAAGTCAT TGATAAACTC 
AAGCACATCA ATCAGCTGCT GCAGGGCAGG TCGATCCCAA AACTGGGCTC TCTGGACCAG 
ATCGAGACGG GCAGCGGAGA CGGAAACAGC GGAGACTGTG ATGATGAGGA CGGATGCTGG 
GGATCAGGGA ACGGAGCAGT CCAGAGGAGA GGGACAGTCA CTAAATTGTT TGGCAGCGAA 
TCCAGACGCT CGCTATGGAA AAACAAAAGG CGTCAGCGAG GCGTATCGGC AGGGAGTCGC 
ATTGATCTCC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289165.1
CDS	1..1635
Translation	MEMAIRGILFYTFFAVFTDVCQGNLMDCEEVRKVFQLKQIGPAKSIPDTPRTGSDLQVCLSRNLTCCTKKMEERYQVAARRDIQNLLQISSSSLKFLFSRNVAAFQESVESLVRQAENHTLSILHQSHRDLSSPAASAPVGEFFTEVALFILGSELSLEESSQSFFNALFPLIYERLIDSRLSPPYAECLRSSAQDLAPFGAAPIRLASQIAHASLPARVFLQALHLGIEIINTTDHIQLSRECRRGLLRMSYCPHCQALTSSKPCMGYCLNVVRGCLANLAEVDAHWREFVRSLETLAGRMHGELDLEQVLLGAHELVRDAVAHAQRSTARIHAQVQRVCGQSSRWTEPVNSVQHSTGRASDSIPLRGANRNTEDSLSSRRTEFLHSLRLYRTFYGALADQLCISELASADGRACWNGDDIARSYTLRVVGNGIKAQAENPEVKVKEADPVINQVIDKLKHINQLLQGRSIPKLGSLDQIETGSGDGNSGDCDDEDGCWGSGNGAVQRRGTVTKLFGSESRRSLWKNKRRQRGVSAGSRIDLQ

Target ORF information:

RefSeq Version	NM_001302236.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glypican 5a (gpc5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302236.1

ORF Insert Sequence:

ATGGAAATGG CGATCCGTGG AATTTTGTTT TATACATTTT TTGCGGTTTT CACAGATGTC 
TGTCAGGGGA ATTTAATGGA CTGCGAAGAA GTGCGTAAAG TTTTTCAGTT GAAACAAATA 
GGTCCTGCAA AATCAATACC TGACACGCCG AGAACAGGTT CAGACCTGCA GGTTTGCTTA 
TCCAGGAATC TAACATGCTG CACCAAAAAG ATGGAGGAGC GTTACCAGGT GGCGGCCCGG 
CGGGATATTC AGAACCTTCT CCAGATCTCT AGCTCCAGCC TAAAGTTCCT CTTCTCACGA 
AATGTGGCCG CTTTCCAAGA AAGCGTGGAG TCTCTGGTGC GTCAGGCGGA GAACCACACC 
CTCTCCATCC TCCATCAGTC CCACCGTGAT CTCTCCAGCC CAGCAGCATC AGCACCAGTC 
GGGGAGTTTT TCACAGAAGT GGCTCTGTTC ATCCTGGGCT CTGAGCTCAG TCTAGAAGAG 
TCCTCTCAGA GCTTCTTCAA CGCTCTTTTC CCCTTAATAT ACGAGCGTCT GATTGACTCC 
CGTCTGTCTC CTCCATACGC CGAATGCCTG CGCTCGTCCG CTCAAGATCT AGCACCGTTC 
GGAGCAGCCC CAATCCGCCT GGCGTCCCAA ATCGCCCATG CTTCTCTACC TGCTCGCGTG 
TTTCTCCAAG CGCTCCATTT GGGCATCGAG ATCATAAATA CGACCGATCA CATCCAGCTG 
AGCCGCGAGT GCAGACGTGG CCTGCTGAGG ATGAGCTACT GCCCGCACTG CCAGGCTCTG 
ACCTCAAGCA AACCCTGCAT GGGATACTGC CTGAACGTGG TGCGCGGGTG TCTGGCTAAC 
CTGGCTGAAG TGGATGCCCA CTGGAGGGAG TTTGTACGCT CGCTGGAGAC GCTGGCAGGA 
CGGATGCACG GAGAGCTGGA TCTGGAGCAG GTGCTGCTGG GGGCGCATGA GCTGGTGAGG 
GACGCTGTGG CGCACGCTCA GAGGAGCACA GCGAGGATAC ATGCGCAGGT GCAACGTGTG 
TGTGGTCAGT CCAGCAGATG GACGGAGCCG GTGAACAGCG TCCAGCACAG CACAGGACGA 
GCATCAGACT CGATACCACT GAGAGGAGCA AACAGAAACA CAGAGGACTC GCTCAGCAGC 
CGCAGAACTG AGTTCCTCCA CAGTCTGCGT TTATATCGCA CTTTTTACGG CGCTCTGGCT 
GATCAGCTGT GCATCAGTGA ACTGGCCTCT GCAGACGGAC GGGCCTGCTG GAACGGAGAC 
GACATCGCCA GAAGCTATAC TCTGCGAGTG GTTGGCAATG GGATTAAAGC GCAGGCTGAA 
AACCCAGAAG TCAAAGTGAA GGAGGCCGAT CCGGTGATCA ATCAAGTCAT TGATAAACTC 
AAGCACATCA ATCAGCTGCT GCAGGGCAGG TCGATCCCAA AACTGGGCTC TCTGGACCAG 
ATCGAGACGG GCAGCGGAGA CGGAAACAGC GGAGACTGTG ATGATGAGGA CGGATGCTGG 
GGATCAGGGA ACGGAGCAGT CCAGAGGAGA GGGACAGTCA CTAAATTGTT TGGCAGCGAA 
TCCAGACGCT CGCTATGGAA AAACAAAAGG CGTCAGCGAG GCGTATCGGC AGGGAGTCGC 
ATTGATCTCC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Identification and expression analysis of zebrafish glypicans during embryonic development.
PloS one8(11)e80824(2013)
Gupta M,Brand M