ADCY1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADCY1A
Entrez Gene ID	557026
Full Name	adenylate cyclase 1a
Synonyms	si:dkey-162m2.1
General protein information	Preferred Names adenylate cyclase 1a Names adenylate cyclase type 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001168349.1	NP_001161821.1	adenylate cyclase type 1
XM_017352482.1	XP_017207971.1	adenylate cyclase type 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ADCY1	XP_519081.3
	ADCY1	XP_001086268.1
Mus musculus (house mouse)	Adcy1	NP_033752.1
Gallus gallus (chicken)	ADCY1	XP_418883.4
Xenopus tropicalis (tropical clawed frog)	adcy1	XP_002933461.1
Danio rerio (zebrafish)	adcy1a	NP_001161821.1
Homo sapiens (human)	ADCY1	NP_066939.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000727	XP_559367.4
Canis lupus familiaris (dog)	ADCY1	XP_849081.3
Bos taurus (cattle)	ADCY1	NP_776654.1
Rattus norvegicus (Norway rat)	Adcy1	NP_001100709.1
Drosophila melanogaster (fruit fly)	rut	NP_511156.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Lamprey IGF-Binding Protein-3 Has IGF-Dependent and -Independent Actions.
Zhong Y, Duan C
Frontiers in endocrinology7174(2016)

Adenylate cyclase 1 (ADCY1) mutations cause recessive hearing impairment in humans and defects in hair cell function and hearing in zebrafish.
Santos-Cortez RL, Lee K, Giese AP, Ansar M, Amin-Ud-Din M, Rehn K, Wang X, Aziz A, Chiu I, Hussain Ali R, Smith JD, , Shendure J, Bamshad M, Nickerson DA, Ahmed ZM, Ahmad W, Riazuddin S, Leal SM
Human molecular genetics23(12)3289-98(2014 Jun)

The calmodulin-stimulated adenylate cyclase ADCY8 sets the sensitivity of zebrafish retinal axons to midline repellents and is required for normal midline crossing.
Xu H, Leinwand SG, Dell AL, Fried-Cassorla E, Raper JA
The Journal of neuroscience : the official journal of the Society for Neuroscience30(21)7423-33(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate that ADCY1 has an evolutionarily conserved role in hearing.
Title: Adenylate cyclase 1 (ADCY1) mutations cause recessive hearing impairment in humans and defects in hair cell function and hearing in zebrafish.

The following ADCY1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADCY1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa56563	XM_017352482.1 Latest version!	Danio rerio adenylate cyclase 1a (adcy1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa33824	NM_001168349.1 Latest version!	Danio rerio adenylate cyclase 1a (adcy1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADCY1A ( XM_017352482.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207971.1 Danio rerio adenylate cyclase 1a (adcy1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56563
Clone ID Related Accession (Same CDS sequence)	XM_017352482.1
Accession Version	XM_017352482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	adenylate cyclase type 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGCC CCGTTATGGA GGAAGTTCCG CTTCCCAGAG TCCGCACGCG GCGGAGGAAA 
AGTCGCTGCC TGGCCGGAGC GTGCGAGCGC GACTTTGACT GCAAGGAGCT CGAGACTCTC 
TTCCAGAAGT ACAACCTGAA GCTGGAGCAA ACCGCCACGC TCAAAGCGCT GGCCATCCTC 
ATCCTCGCCG CGTCCGCGCT GGCGCTCGTG GAGCTGCTGT CCGCCGGCTC GCGCTTCACC 
GTGTCCAAGG GCTCGCACCC TGTCCACTGC GTCATCTTCA CCTCACTCTT CATCGTCACT 
AACGTCAAAT ACCTGCAGGT CACGCAGCTG CAGCAGATCG CGCGCCTGGC GCTGCTCTTC 
AGCTTCACGT TCGCGCTGCT CTGTTGCCCG TTTCCCCTTG CTCTGGGTCA CGGGACGCAG 
GGCACGCACG GGCCTGACGC GCCGAGCACA ACACCTGAGC AAGGTGTGTG GCAGCTGATG 
CTCGTCACCT TCGTTGCCTA CTCTCTGCTC CCAGTCAGGA CGCTCCTGGC GGTTTTGTTC 
GGCGTCCTGG TGGCTGCGTC GCATTTCATC GTCACGGCGA CATCGGTAAC TGCGCGAAGA 
CCTCGCGTGT GGACTCCGCT GGTAGCAAAC GCAGTGCTGC TGGTCGGTGT GAATCTGTCG 
GGTGTGTTTG TGCGAGTCCG TACTGAGCGT GCCCAGAGGA AGGCCTTTCT ACAAGCCAGA 
AACTGCATCC AACAGCGCCT GCAACTAGAG GACGAGAACG AGAAACAGGA ACGCCTCCTG 
ATGAGCCTGC TGCCCAGAAA TGTTGCAATG GAGATGAAGG AGGACTTCCT GAAGCCACCA 
GAGAGAATCT TCCACAAGAT CTACATCCAG CGGCACGACA ATGTCAGCAT CCTGTTTGCA 
GACATTGTGG GTTTCACCAG TCTGGCATCT CAGTGCACTG CGCAGGAGCT GGTCAAACTG 
CTCAACGAGC TCTTCGGCAA ATTTGATGAG CTCGCTACAG AAAACCACTG CCGGAGGATC 
AAGATCTTGG GCGACTGTTA TTACTGTGTG TCTGGCCTGA CTCAACCCAA AGCAGACCAC 
GCACACTGCT GTGTAGAGAT GGGTCTGGAC ATGATCGACA CCATCACGTC TGTGGCAGAG 
GCGACTGAGG TGGACTTGAA CATGCGTGTT GGTTTGCACA CAGGCCGTGT GCTGTGTGGT 
GTTCTGGGGC TCCGCAAATG GCAGTATGAC GTCTGGTCCA ATGATGTTAC ACTGGCCAAC 
ATGATGGAGG CAGGAGGACT GCCCGGGAAG GTGCACATCA CAAAGGCGAC GCTCGAATGT 
CTGAATGGAG ATTATGAGGT GGAGCCAGGA TTTGGTCATG AGAGACATGC TTTCCTCAGC 
AAACATGCCA TCGAGACCTT CTTCATCGTG CCCTCACACA GACGCAAGAT TTTTCCTGGG 
CTGATCCTAT CAGACATAAA GCCGGCGAAA AGGATGAAGT TTAAGACCGT CTGTTACCTG 
CTGGTGCAGC TCATGCACTG CAGAAAGATG TTCAAGGCTG AGATCCCTTT CTCCAACGTC 
ATGACCTGTG AGGACGATGA CAAGAGACGA GCGTTACGAA CGGCTTCTGA GAAGCTTCGT 
AACCGGACGT CATTTTCAGC AGGAATGGTG CAGAATTCTC CAGGGACCCG AGTGAACCGT 
TATATCGGCC GGCTGATAGA AGCACGACAG ACGGAGTCAG AGATGGCAGA CCTCAACTAC 
ATCACCCTCA CATACAGGAG CAGAGAAAGA GAGCAGAGAT ACCAGCAAGT GTATGATGAT 
GCCTTCATCA GTGCGGTGGT TATCTCGCTA GTCCTCACTT CGTTTTTTGC AGTGGTCTAT 
TTTCTCATCA TTCCTCAAGG TCTTCTGGTT GTAATTCTGC TGGTTCTCTG TTTATGTTTC 
CACATGGCAA GTGTGATGTA TCTTCACGTT ACGCATGTGC AGTGCTCCCC CGACTGCCTG 
ACCCTACAGA TCAGAACGGT TTTGTGTGGG TTTCTGGTGC TGCTGATGTA CTCTGTGACC 
CAGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207971.1
CDS	1233..3281
Translation	MNGPVMEEVPLPRVRTRRRKSRCLAGACERDFDCKELETLFQKYNLKLEQTATLKALAILILAASALALVELLSAGSRFTVSKGSHPVHCVIFTSLFIVTNVKYLQVTQLQQIARLALLFSFTFALLCCPFPLALGHGTQGTHGPDAPSTTPEQGVWQLMLVTFVAYSLLPVRTLLAVLFGVLVAASHFIVTATSVTARRPRVWTPLVANAVLLVGVNLSGVFVRVRTERAQRKAFLQARNCIQQRLQLEDENEKQERLLMSLLPRNVAMEMKEDFLKPPERIFHKIYIQRHDNVSILFADIVGFTSLASQCTAQELVKLLNELFGKFDELATENHCRRIKILGDCYYCVSGLTQPKADHAHCCVEMGLDMIDTITSVAEATEVDLNMRVGLHTGRVLCGVLGLRKWQYDVWSNDVTLANMMEAGGLPGKVHITKATLECLNGDYEVEPGFGHERHAFLSKHAIETFFIVPSHRRKIFPGLILSDIKPAKRMKFKTVCYLLVQLMHCRKMFKAEIPFSNVMTCEDDDKRRALRTASEKLRNRTSFSAGMVQNSPGTRVNRYIGRLIEARQTESEMADLNYITLTYRSREREQRYQQVYDDAFISAVVISLVLTSFFAVVYFLIIPQGLLVVILLVLCLCFHMASVMYLHVTHVQCSPDCLTLQIRTVLCGFLVLLMYSVTQG

Target ORF information:

RefSeq Version	XM_017352482.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio adenylate cyclase 1a (adcy1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352482.1

ORF Insert Sequence:

ATGAATGGCC CCGTTATGGA GGAAGTTCCG CTTCCCAGAG TCCGCACGCG GCGGAGGAAA 
AGTCGCTGCC TGGCCGGAGC GTGCGAGCGC GACTTTGACT GCAAGGAGCT CGAGACTCTC 
TTCCAGAAGT ACAACCTGAA GCTGGAGCAA ACCGCCACGC TCAAAGCGCT GGCCATCCTC 
ATCCTCGCCG CGTCCGCGCT GGCGCTCGTG GAGCTGCTGT CCGCCGGCTC GCGCTTCACC 
GTGTCCAAGG GCTCGCACCC TGTCCACTGC GTCATCTTCA CCTCACTCTT CATCGTCACT 
AACGTCAAAT ACCTGCAGGT CACGCAGCTG CAGCAGATCG CGCGCCTGGC GCTGCTCTTC 
AGCTTCACGT TCGCGCTGCT CTGTTGCCCG TTTCCCCTTG CTCTGGGTCA CGGGACGCAG 
GGCACGCACG GGCCTGACGC GCCGAGCACA ACACCTGAGC AAGGTGTGTG GCAGCTGATG 
CTCGTCACCT TCGTTGCCTA CTCTCTGCTC CCAGTCAGGA CGCTCCTGGC GGTTTTGTTC 
GGCGTCCTGG TGGCTGCGTC GCATTTCATC GTCACGGCGA CATCGGTAAC TGCGCGAAGA 
CCTCGCGTGT GGACTCCGCT GGTAGCAAAC GCAGTGCTGC TGGTCGGTGT GAATCTGTCG 
GGTGTGTTTG TGCGAGTCCG TACTGAGCGT GCCCAGAGGA AGGCCTTTCT ACAAGCCAGA 
AACTGCATCC AACAGCGCCT GCAACTAGAG GACGAGAACG AGAAACAGGA ACGCCTCCTG 
ATGAGCCTGC TGCCCAGAAA TGTTGCAATG GAGATGAAGG AGGACTTCCT GAAGCCACCA 
GAGAGAATCT TCCACAAGAT CTACATCCAG CGGCACGACA ATGTCAGCAT CCTGTTTGCA 
GACATTGTGG GTTTCACCAG TCTGGCATCT CAGTGCACTG CGCAGGAGCT GGTCAAACTG 
CTCAACGAGC TCTTCGGCAA ATTTGATGAG CTCGCTACAG AAAACCACTG CCGGAGGATC 
AAGATCTTGG GCGACTGTTA TTACTGTGTG TCTGGCCTGA CTCAACCCAA AGCAGACCAC 
GCACACTGCT GTGTAGAGAT GGGTCTGGAC ATGATCGACA CCATCACGTC TGTGGCAGAG 
GCGACTGAGG TGGACTTGAA CATGCGTGTT GGTTTGCACA CAGGCCGTGT GCTGTGTGGT 
GTTCTGGGGC TCCGCAAATG GCAGTATGAC GTCTGGTCCA ATGATGTTAC ACTGGCCAAC 
ATGATGGAGG CAGGAGGACT GCCCGGGAAG GTGCACATCA CAAAGGCGAC GCTCGAATGT 
CTGAATGGAG ATTATGAGGT GGAGCCAGGA TTTGGTCATG AGAGACATGC TTTCCTCAGC 
AAACATGCCA TCGAGACCTT CTTCATCGTG CCCTCACACA GACGCAAGAT TTTTCCTGGG 
CTGATCCTAT CAGACATAAA GCCGGCGAAA AGGATGAAGT TTAAGACCGT CTGTTACCTG 
CTGGTGCAGC TCATGCACTG CAGAAAGATG TTCAAGGCTG AGATCCCTTT CTCCAACGTC 
ATGACCTGTG AGGACGATGA CAAGAGACGA GCGTTACGAA CGGCTTCTGA GAAGCTTCGT 
AACCGGACGT CATTTTCAGC AGGAATGGTG CAGAATTCTC CAGGGACCCG AGTGAACCGT 
TATATCGGCC GGCTGATAGA AGCACGACAG ACGGAGTCAG AGATGGCAGA CCTCAACTAC 
ATCACCCTCA CATACAGGAG CAGAGAAAGA GAGCAGAGAT ACCAGCAAGT GTATGATGAT 
GCCTTCATCA GTGCGGTGGT TATCTCGCTA GTCCTCACTT CGTTTTTTGC AGTGGTCTAT 
TTTCTCATCA TTCCTCAAGG TCTTCTGGTT GTAATTCTGC TGGTTCTCTG TTTATGTTTC 
CACATGGCAA GTGTGATGTA TCTTCACGTT ACGCATGTGC AGTGCTCCCC CGACTGCCTG 
ACCCTACAGA TCAGAACGGT TTTGTGTGGG TTTCTGGTGC TGCTGATGTA CTCTGTGACC 
CAGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADCY1A ( NM_001168349.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001161821.1 Danio rerio adenylate cyclase 1a (adcy1a), mRNA.

Price & Availability↑

CloneID	ODa33824
Clone ID Related Accession (Same CDS sequence)	NM_001168349.1
Accession Version	NM_001168349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	adenylate cyclase type 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU169394.1. ##Evidence-Data-START## Transcript exon combination :: GU169394.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAATGGCC CCGCTATGGA GGAAGTTCCG CTTCCCAGAG TCCGCACGCG GCGGAGGAAA 
AGTCGCTGCC TGGCCGGAGC GTGCGAGCGC GACTTTGACT GCAAGGAGCT CGAGACTCTC 
TTCCAGAAGT ACAACCTGAA GCTGGAGCAA ACCGCCACGC TCAAAGCGCT GGCCATCCTC 
ATCCTCGCCG CGTCCGCGCT GGCGCTCGTG GAGCTGCTGT CCGCCGGCTC GCGCTTCACC 
GTGTCCAAGG GCTCGCACCC TGTCCACTGC GTCATCTTCA CCTCACTCTT CATCGTCACT 
AACGTCAAAT ACCTGCAGGT CACGCAGCTG CAGCAGATCG CGCGCCTGGC GCTGCTCTTC 
AGCTTCACGT TCGCGCTGCT CTGTTGCCCG TTTCCCCTTG CTCTGGGTCA CGGGACGCAG 
GGCACGCACG GGCCTGACGC GCCGAGCACA ACACCTGAGC AAGGTGTGTG GCAGCTGATG 
CTCGTCACCT TCGTTGCCTA CTCTCTGCTC CCAGTCAGGA CGCTCCTGGC GGTTTTGTTC 
GGCGTCCTGG TGGCTGCGTC GCATTTCATC GTCACGGCGA CATCGGTAAC TGCGCGAAGA 
CCTCGCGTGT GGACTCCGCT GGTAGCAAAC GCAGTGCTGC TGGTCGGTGT GAATCTGTCG 
GGTGTGTTTG TGCGAGTCCG TACTGAGCGT GCCCAGAGGA AGGCCTTCCT ACAAGCCAGA 
AACTGCATCC AACAGCGCCT GCAACTAGAG GACGAGAACG AGAAACAGGA ACGCCTCCTG 
ATGAGCCTGC TGCCCAGAAA TGTTGCAATG GAGATGAAGG AGGACTTCCT GAAGCCACCA 
GAGAGAATCT TCCACAAGAT CTACATCCAG CGGCACGACA ATGTCAGCAT CCTGTTTGCA 
GACATTGTGG GTTTCACCAG TCTGGCATCT CAGTGCACTG CGCAGGAGCT GGTCAAACTG 
CTCAACGAGC TCTTCGGCAA ATTTGATGAG CTCGCTACAG AAAACCACTG CCGGAGGATC 
AAGATCTTGG GCGACTGTTA TTACTGTGTG TCTGGCCTGA CTCAACCCAA AGCAGACCAC 
GCACACTGCT GTGTAGAGAT GGGTCTGGAC ATGATCGACA CCATCACGTC TGTGGCAGAG 
GCGACTGAGG TGGACTTGAA CATGCGTGTT GGTTTGCACA CAGGCCGTGT GCTGTGTGGT 
GTTCTGGGGC TCCGCAAATG GCAGTACGAC GTCTGGTCCA ATGATGTTAC ACTGGCCAAC 
ATGATGGAGG CAGGAGGACT GCCCGGGAAG GTGCACATCA CAAAGGCGAC GCTCGAATGT 
CTGAATGGAG ATTATGAGGT GGAGCCAGGA TTTGGTCATG AGAGACATGC TTTCCTCAGC 
AAACATGCCA TCGAGACCTT CTTCATCGTG CCCTCACACA GACGCAAGAT TTTTCCTGGG 
CTGATCCTAT CAGACATAAA GCCGGCGAAA AGGATGGAGT TTAAGACCGT CTGTTACCTG 
CTGGTGCAGC TCATGCACTG CAGAAAGATG TTCAAGGCTG AGATCCCTTT CTCCAACGTC 
ATGACCTGTG AGGACGATGA CAAGAGACGA GCGTTACGAA CGGCTTCTGA GAAGCTTCGT 
AACCGGACGT CATTTTCAGC AGGAATGGTG CAGAATTCTC CAGGGACCCG AGTGAACCGT 
TATATCGGCC GGCTGATAGA AGCACGACAG ACGGAGTCAG AGATGGCAGA CCTCAACTAC 
ATCACCCTCA CATACAGGAG CAGAGAAAGA GAGCAGAGAT ACCTGCAAGT GTATGATGAT 
GCCTTCATCA GTGCGGTGGT TATCTCGCTA GTCCTCACTT CGTTTTTTGC AGTGGTCTAT 
TTTCTCATCA TTCCTCAAGG TCTTCTGGTT GTAATTCTGC TGGTTCTCTG TTTATGTTTC 
CACATGGCAA GTGTGATGTA TCTTCACGTT ACCCATGTAC AGTGCTCCCC CGACTGCCTG 
ACCCTACAGA TCAGAACGGT TTTGTGTGGG TTTCTGGTGC TGCTGATGTA CTCTGTGACC 
CAGGGGTGTG TGGTGACATG CGTGCCGTGG TCACTGGAGG GGAATTCCAG CATCTCCATG 
GTGACAGTTG TCACAGTGAA CGGGACCGGC GTGGGCTACA GGGTGTGCAA ATACACTCCG 
TATGTATTTT TATCATGTGT GATGGCATCT CTATCTGTGG CCCTGTACCT ACGTGTCTCA 
TCACTACCCA AGATCCTCCT GCTTCTGCTT CTTAACATCC TCCACACTGT CGTCATGGAG 
ACCAGCGGCT ATCGGAAGGC CGTAGGGGGT GGATTCTTTC ACACACATGG CTATGACCCG 
GTTCTAGCGA TGCTGTTGTT TTCTGGAGCT CTGGTGCTTC ATTGTAGACA GCTGGATCTC 
AAGCTTAGAC TGGACTACCT CTGGGCCACA CAGGCTGAGG AGGAGAGAGA TGACATGGAG 
AGAGTGAAGC TGGACAATAA GAGGATTCTC TTCAACCTGC TGCCTGCTCA TGTCGCTCAA 
CACTTCCTGC TGTCCAACCC GCGAAACATG GACCTGTATT ATCAGTCGTA CTCTCAAGTG 
GGCGTCCTGT TCGCCTCCAT CCCCAACTTT AATGACTTCT ACATTGAGCT GGATGGAAAC 
AACATGGGTG TGGAGTGCCT CCGGCTGCTC AACGAGATCA TCGCCGACTT TGATGCGCTG 
ATGGATAAGG AATGCTACAG AGACATTGAG AAGATCAAAA CTATTGGAAG TACGTACATG 
GCTGCTGTCG GACTCGTCCC TACTGAAGGA TCTAAGGTAA AGAAGTCCAT TTCAGCGCAC 
CTCTGCACAG TGGCTGATTA TGCCATCGAG ATGTTTGACG TCCTGGATGC CATCAACTAC 
CAGTCCTACA ATGACTTTGT GCTTCGAGTT GGTATTAATG TGGGTCCAGT GGTTGCCGGA 
GTAATCGGGG CTCGCAGGCC TCAATACGAC ATCTGGGGAA ACACAGTCAA CGTCGCCAGC 
AGAATGGACA GCACCGGAGT TCAGGGAAAG ATACAGGTGA CAGAGGACGT CTTCCGCCTA 
CTCTCGGGTT ATTATGACTT CAACTGTCGT GGCCAAGTCA GCGTAAAGGG CAAAGGTCAA 
ATGCTCACCT ACTTTCTGGA GGGCCGAACC CCTCAAGGTG CCCAGCACAG CCGATCCAAG 
AGTCCAGATC AAGACTCGAG TCCCTGCATC CGGACCAAAC TGGGCTCCGC ACCCGCCGTC 
AGCAGCTACG GCGTAAAGAG TCACACCGTG GGACACGTGG GCACCTCAAA CAACAGCATT 
CTGTACCTGC CCTCAGTGTC CGTCGACATG GAGATGGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161821.1
CDS	381..3725
Translation	MNGPAMEEVPLPRVRTRRRKSRCLAGACERDFDCKELETLFQKYNLKLEQTATLKALAILILAASALALVELLSAGSRFTVSKGSHPVHCVIFTSLFIVTNVKYLQVTQLQQIARLALLFSFTFALLCCPFPLALGHGTQGTHGPDAPSTTPEQGVWQLMLVTFVAYSLLPVRTLLAVLFGVLVAASHFIVTATSVTARRPRVWTPLVANAVLLVGVNLSGVFVRVRTERAQRKAFLQARNCIQQRLQLEDENEKQERLLMSLLPRNVAMEMKEDFLKPPERIFHKIYIQRHDNVSILFADIVGFTSLASQCTAQELVKLLNELFGKFDELATENHCRRIKILGDCYYCVSGLTQPKADHAHCCVEMGLDMIDTITSVAEATEVDLNMRVGLHTGRVLCGVLGLRKWQYDVWSNDVTLANMMEAGGLPGKVHITKATLECLNGDYEVEPGFGHERHAFLSKHAIETFFIVPSHRRKIFPGLILSDIKPAKRMEFKTVCYLLVQLMHCRKMFKAEIPFSNVMTCEDDDKRRALRTASEKLRNRTSFSAGMVQNSPGTRVNRYIGRLIEARQTESEMADLNYITLTYRSREREQRYLQVYDDAFISAVVISLVLTSFFAVVYFLIIPQGLLVVILLVLCLCFHMASVMYLHVTHVQCSPDCLTLQIRTVLCGFLVLLMYSVTQGCVVTCVPWSLEGNSSISMVTVVTVNGTGVGYRVCKYTPYVFLSCVMASLSVALYLRVSSLPKILLLLLLNILHTVVMETSGYRKAVGGGFFHTHGYDPVLAMLLFSGALVLHCRQLDLKLRLDYLWATQAEEERDDMERVKLDNKRILFNLLPAHVAQHFLLSNPRNMDLYYQSYSQVGVLFASIPNFNDFYIELDGNNMGVECLRLLNEIIADFDALMDKECYRDIEKIKTIGSTYMAAVGLVPTEGSKVKKSISAHLCTVADYAIEMFDVLDAINYQSYNDFVLRVGINVGPVVAGVIGARRPQYDIWGNTVNVASRMDSTGVQGKIQVTEDVFRLLSGYYDFNCRGQVSVKGKGQMLTYFLEGRTPQGAQHSRSKSPDQDSSPCIRTKLGSAPAVSSYGVKSHTVGHVGTSNNSILYLPSVSVDMEMEV

Target ORF information:

RefSeq Version	NM_001168349.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenylate cyclase 1a (adcy1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168349.1

ORF Insert Sequence:

ATGAATGGCC CCGCTATGGA GGAAGTTCCG CTTCCCAGAG TCCGCACGCG GCGGAGGAAA 
AGTCGCTGCC TGGCCGGAGC GTGCGAGCGC GACTTTGACT GCAAGGAGCT CGAGACTCTC 
TTCCAGAAGT ACAACCTGAA GCTGGAGCAA ACCGCCACGC TCAAAGCGCT GGCCATCCTC 
ATCCTCGCCG CGTCCGCGCT GGCGCTCGTG GAGCTGCTGT CCGCCGGCTC GCGCTTCACC 
GTGTCCAAGG GCTCGCACCC TGTCCACTGC GTCATCTTCA CCTCACTCTT CATCGTCACT 
AACGTCAAAT ACCTGCAGGT CACGCAGCTG CAGCAGATCG CGCGCCTGGC GCTGCTCTTC 
AGCTTCACGT TCGCGCTGCT CTGTTGCCCG TTTCCCCTTG CTCTGGGTCA CGGGACGCAG 
GGCACGCACG GGCCTGACGC GCCGAGCACA ACACCTGAGC AAGGTGTGTG GCAGCTGATG 
CTCGTCACCT TCGTTGCCTA CTCTCTGCTC CCAGTCAGGA CGCTCCTGGC GGTTTTGTTC 
GGCGTCCTGG TGGCTGCGTC GCATTTCATC GTCACGGCGA CATCGGTAAC TGCGCGAAGA 
CCTCGCGTGT GGACTCCGCT GGTAGCAAAC GCAGTGCTGC TGGTCGGTGT GAATCTGTCG 
GGTGTGTTTG TGCGAGTCCG TACTGAGCGT GCCCAGAGGA AGGCCTTCCT ACAAGCCAGA 
AACTGCATCC AACAGCGCCT GCAACTAGAG GACGAGAACG AGAAACAGGA ACGCCTCCTG 
ATGAGCCTGC TGCCCAGAAA TGTTGCAATG GAGATGAAGG AGGACTTCCT GAAGCCACCA 
GAGAGAATCT TCCACAAGAT CTACATCCAG CGGCACGACA ATGTCAGCAT CCTGTTTGCA 
GACATTGTGG GTTTCACCAG TCTGGCATCT CAGTGCACTG CGCAGGAGCT GGTCAAACTG 
CTCAACGAGC TCTTCGGCAA ATTTGATGAG CTCGCTACAG AAAACCACTG CCGGAGGATC 
AAGATCTTGG GCGACTGTTA TTACTGTGTG TCTGGCCTGA CTCAACCCAA AGCAGACCAC 
GCACACTGCT GTGTAGAGAT GGGTCTGGAC ATGATCGACA CCATCACGTC TGTGGCAGAG 
GCGACTGAGG TGGACTTGAA CATGCGTGTT GGTTTGCACA CAGGCCGTGT GCTGTGTGGT 
GTTCTGGGGC TCCGCAAATG GCAGTACGAC GTCTGGTCCA ATGATGTTAC ACTGGCCAAC 
ATGATGGAGG CAGGAGGACT GCCCGGGAAG GTGCACATCA CAAAGGCGAC GCTCGAATGT 
CTGAATGGAG ATTATGAGGT GGAGCCAGGA TTTGGTCATG AGAGACATGC TTTCCTCAGC 
AAACATGCCA TCGAGACCTT CTTCATCGTG CCCTCACACA GACGCAAGAT TTTTCCTGGG 
CTGATCCTAT CAGACATAAA GCCGGCGAAA AGGATGGAGT TTAAGACCGT CTGTTACCTG 
CTGGTGCAGC TCATGCACTG CAGAAAGATG TTCAAGGCTG AGATCCCTTT CTCCAACGTC 
ATGACCTGTG AGGACGATGA CAAGAGACGA GCGTTACGAA CGGCTTCTGA GAAGCTTCGT 
AACCGGACGT CATTTTCAGC AGGAATGGTG CAGAATTCTC CAGGGACCCG AGTGAACCGT 
TATATCGGCC GGCTGATAGA AGCACGACAG ACGGAGTCAG AGATGGCAGA CCTCAACTAC 
ATCACCCTCA CATACAGGAG CAGAGAAAGA GAGCAGAGAT ACCTGCAAGT GTATGATGAT 
GCCTTCATCA GTGCGGTGGT TATCTCGCTA GTCCTCACTT CGTTTTTTGC AGTGGTCTAT 
TTTCTCATCA TTCCTCAAGG TCTTCTGGTT GTAATTCTGC TGGTTCTCTG TTTATGTTTC 
CACATGGCAA GTGTGATGTA TCTTCACGTT ACCCATGTAC AGTGCTCCCC CGACTGCCTG 
ACCCTACAGA TCAGAACGGT TTTGTGTGGG TTTCTGGTGC TGCTGATGTA CTCTGTGACC 
CAGGGGTGTG TGGTGACATG CGTGCCGTGG TCACTGGAGG GGAATTCCAG CATCTCCATG 
GTGACAGTTG TCACAGTGAA CGGGACCGGC GTGGGCTACA GGGTGTGCAA ATACACTCCG 
TATGTATTTT TATCATGTGT GATGGCATCT CTATCTGTGG CCCTGTACCT ACGTGTCTCA 
TCACTACCCA AGATCCTCCT GCTTCTGCTT CTTAACATCC TCCACACTGT CGTCATGGAG 
ACCAGCGGCT ATCGGAAGGC CGTAGGGGGT GGATTCTTTC ACACACATGG CTATGACCCG 
GTTCTAGCGA TGCTGTTGTT TTCTGGAGCT CTGGTGCTTC ATTGTAGACA GCTGGATCTC 
AAGCTTAGAC TGGACTACCT CTGGGCCACA CAGGCTGAGG AGGAGAGAGA TGACATGGAG 
AGAGTGAAGC TGGACAATAA GAGGATTCTC TTCAACCTGC TGCCTGCTCA TGTCGCTCAA 
CACTTCCTGC TGTCCAACCC GCGAAACATG GACCTGTATT ATCAGTCGTA CTCTCAAGTG 
GGCGTCCTGT TCGCCTCCAT CCCCAACTTT AATGACTTCT ACATTGAGCT GGATGGAAAC 
AACATGGGTG TGGAGTGCCT CCGGCTGCTC AACGAGATCA TCGCCGACTT TGATGCGCTG 
ATGGATAAGG AATGCTACAG AGACATTGAG AAGATCAAAA CTATTGGAAG TACGTACATG 
GCTGCTGTCG GACTCGTCCC TACTGAAGGA TCTAAGGTAA AGAAGTCCAT TTCAGCGCAC 
CTCTGCACAG TGGCTGATTA TGCCATCGAG ATGTTTGACG TCCTGGATGC CATCAACTAC 
CAGTCCTACA ATGACTTTGT GCTTCGAGTT GGTATTAATG TGGGTCCAGT GGTTGCCGGA 
GTAATCGGGG CTCGCAGGCC TCAATACGAC ATCTGGGGAA ACACAGTCAA CGTCGCCAGC 
AGAATGGACA GCACCGGAGT TCAGGGAAAG ATACAGGTGA CAGAGGACGT CTTCCGCCTA 
CTCTCGGGTT ATTATGACTT CAACTGTCGT GGCCAAGTCA GCGTAAAGGG CAAAGGTCAA 
ATGCTCACCT ACTTTCTGGA GGGCCGAACC CCTCAAGGTG CCCAGCACAG CCGATCCAAG 
AGTCCAGATC AAGACTCGAG TCCCTGCATC CGGACCAAAC TGGGCTCCGC ACCCGCCGTC 
AGCAGCTACG GCGTAAAGAG TCACACCGTG GGACACGTGG GCACCTCAAA CAACAGCATT 
CTGTACCTGC CCTCAGTGTC CGTCGACATG GAGATGGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Lamprey IGF-Binding Protein-3 Has IGF-Dependent and -Independent Actions.
Frontiers in endocrinology7174(2016)
Zhong Y,Duan C

Adenylate cyclase 1 (ADCY1) mutations cause recessive hearing impairment in humans and defects in hair cell function and hearing in zebrafish.
Human molecular genetics23(12)3289-98(2014 Jun)
Santos-Cortez RL,Lee K,Giese AP,Ansar M,Amin-Ud-Din M,Rehn K,Wang X,Aziz A,Chiu I,Hussain Ali R,Smith JD,,Shendure J,Bamshad M,Nickerson DA,Ahmed ZM,Ahmad W,Riazuddin S,Leal SM

The calmodulin-stimulated adenylate cyclase ADCY8 sets the sensitivity of zebrafish retinal axons to midline repellents and is required for normal midline crossing.
The Journal of neuroscience : the official journal of the Society for Neuroscience30(21)7423-33(2010 May)
Xu H,Leinwand SG,Dell AL,Fried-Cassorla E,Raper JA