ube3b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ube3b
Entrez Gene ID	556864
Full Name	ubiquitin protein ligase E3B
Synonyms	si:dkey-189p24.3
General protein information	Preferred Names ubiquitin protein ligase E3B Names ubiquitin-protein ligase E3B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005165149.4	XP_005165206.1	ubiquitin-protein ligase E3B isoform X1
XM_005165149.3	XP_005165206.1	ubiquitin-protein ligase E3B isoform X1
NM_001114682.1	NP_001108154.1	ubiquitin-protein ligase E3B
XM_005165148.3	XP_005165205.1	ubiquitin-protein ligase E3B isoform X1
XM_005165148.4	XP_005165205.1	ubiquitin-protein ligase E3B isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ube3b	NP_001072875.1
Pan troglodytes (chimpanzee)	UBE3B	XP_001137003.2
Danio rerio (zebrafish)	ube3b	NP_001108154.1
	UBE3B	XP_002798818.1
Homo sapiens (human)	UBE3B	NP_569733.2
Canis lupus familiaris (dog)	UBE3B	XP_543437.2
Bos taurus (cattle)	UBE3B	NP_001193161.1
Drosophila melanogaster (fruit fly)	CG5087	NP_001261610.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005897	XP_315926.2
Caenorhabditis elegans (roundworm)	oxi-1	NP_499392.1
Mus musculus (house mouse)	Ube3b	NP_473434.2
Rattus norvegicus (Norway rat)	Ube3b	NP_001137366.1
Gallus gallus (chicken)	LOC776286	XP_003642246.2

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ube3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10273	NM_001114682.1 Latest version!	Danio rerio ubiquitin protein ligase E3B (ube3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10273	XM_005165149.4 Latest version!	Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10273	XM_005165148.4 Latest version!	Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa10273	XM_005165149.3	Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10273	XM_005165148.3	Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ube3b ( NM_001114682.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001108154.1 Danio rerio ubiquitin protein ligase E3B (ube3b), mRNA.

Price & Availability↑

CloneID	ODa10273
Clone ID Related Accession (Same CDS sequence)	XM_005165149.4 , XM_005165149.3 , NM_001114682.1 , XM_005165148.3 , XM_005165148.4
Accession Version	NM_001114682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	ubiquitin-protein ligase E3B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX510366.5. On Feb 21, 2008 this sequence version replaced XM_679786.2. ##Evidence-Data-START## CDS exon combination :: GDQH01006749.1, GFIL01042576.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA4476738 [ECO:0000348] ##Evidence-Data-END##

ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC 
AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC 
ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT 
AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA 
ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT 
CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA 
TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG 
TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG 
GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT 
CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA 
CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC 
GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA 
GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT 
GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT 
GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC 
TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG 
TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA 
GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA 
ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT 
CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA 
TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT 
GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG 
GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG 
CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC 
TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG 
GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC 
ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG 
CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT 
TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG 
GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG 
GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG 
CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC 
ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT 
GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG 
CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA 
GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC 
AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA 
TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG 
AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT 
TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG 
CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT 
GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT 
CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT 
ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC 
ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG 
GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC 
ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC 
CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC 
TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG 
GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC 
ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT 
CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC 
AGCATGAACA CTGGCTTTGA GCTGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108154.1
CDS	8..3217
Translation	MFTATQTSKTQFLDKARVAREERKGHKEKERAATQIQALVRRFLCRCRLQKEIRKEVDDFIAVSDAGPTKRNALSIFKIARKLLFILSKDDKLRFEKLCRIILNSMDVENEPKVWYVSLALSKDLTILWLKQIKDVLWVSCEFLKKLKPDILQDNKLVTLYLTMLITFTDTSTWKIIRGKGEALKPALNRICENIMGHLNQKGFYSVLQILLTNGLARSRPSLSKGTLTAIFTLSLRPVIAAHFSDNLLRSFLLHVMSVPAFISHLNTLTPDCMITIQTHDLFRKFVLFLSREEQCLDICVCLEGSHTLCLLGNLIQLGYFNVKVLEQEAGHFVKDLTDMLSYCQRYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLVTKQLQCLWGVPVICTLFCDVLSKKLETQDPTPPPAPQPSSLQNNLPVKNLFKRAFQKSASVRNILKPVGGKRVDSAEVQKVCSICVLYQTALTTLTQIRLQILTGLTYLDELLPKLWAFICELGPQGGLRLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKMEELVTISSFLNTFVYKMVWDGILENAKGEKLDLFHSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSLLFQELEKGKKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKETLGLVETSSASPHVTHITIRRSRMLEDGYDQLRRLPVNSIKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKKVFNPALNLFKTTSGNERLYPSPTSSIHENHLQLFEFVGKMLGKAMYEGIVVDVPFASFFLSQVLGHHHSTFYSSIDELPSLDSEFYKNLTSIKRYDGDVSDLGLTLSYDEEVMGQLVCHELIPGGKTMPVTNENKISYIHLMAHFRMHTQIKEQTAAFIRGFRSIINPEWLHMFSTPEVQRLISGDNAEIDLDDLKKHTVYYGGFHSSHRVILWLWDILSSDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKSILRDKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	NM_001114682.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3B (ube3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114682.1

ORF Insert Sequence:

ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC 
AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC 
ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT 
AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA 
ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT 
CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA 
TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG 
TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG 
GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT 
CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA 
CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC 
GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA 
GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT 
GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT 
GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC 
TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG 
TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA 
GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA 
ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT 
CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA 
TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT 
GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG 
GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG 
CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC 
TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG 
GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC 
ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG 
CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT 
TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG 
GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG 
GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG 
CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC 
ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT 
GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG 
CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA 
GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC 
AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA 
TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG 
AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT 
TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG 
CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT 
GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT 
CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT 
ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC 
ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG 
GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC 
ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC 
CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC 
TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG 
GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC 
ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT 
CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC 
AGCATGAACA CTGGCTTTGA GCTGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3b ( XM_005165149.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165206.1 Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10273
Clone ID Related Accession (Same CDS sequence)	XM_005165149.4 , XM_005165149.3 , NM_001114682.1 , XM_005165148.3 , XM_005165148.4
Accession Version	XM_005165149.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165149.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC 
AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC 
ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT 
AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA 
ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT 
CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA 
TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG 
TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG 
GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT 
CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA 
CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC 
GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA 
GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT 
GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT 
GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC 
TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG 
TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA 
GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA 
ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT 
CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA 
TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT 
GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG 
GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG 
CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC 
TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG 
GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC 
ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG 
CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT 
TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG 
GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG 
GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG 
CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC 
ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT 
GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG 
CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA 
GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC 
AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA 
TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG 
AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT 
TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG 
CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT 
GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT 
CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT 
ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC 
ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG 
GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC 
ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC 
CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC 
TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG 
GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC 
ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT 
CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC 
AGCATGAACA CTGGCTTTGA GCTGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165206.1
CDS	275..3484
Translation	MFTATQTSKTQFLDKARVAREERKGHKEKERAATQIQALVRRFLCRCRLQKEIRKEVDDFIAVSDAGPTKRNALSIFKIARKLLFILSKDDKLRFEKLCRIILNSMDVENEPKVWYVSLALSKDLTILWLKQIKDVLWVSCEFLKKLKPDILQDNKLVTLYLTMLITFTDTSTWKIIRGKGEALKPALNRICENIMGHLNQKGFYSVLQILLTNGLARSRPSLSKGTLTAIFTLSLRPVIAAHFSDNLLRSFLLHVMSVPAFISHLNTLTPDCMITIQTHDLFRKFVLFLSREEQCLDICVCLEGSHTLCLLGNLIQLGYFNVKVLEQEAGHFVKDLTDMLSYCQRYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLVTKQLQCLWGVPVICTLFCDVLSKKLETQDPTPPPAPQPSSLQNNLPVKNLFKRAFQKSASVRNILKPVGGKRVDSAEVQKVCSICVLYQTALTTLTQIRLQILTGLTYLDELLPKLWAFICELGPQGGLRLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKMEELVTISSFLNTFVYKMVWDGILENAKGEKLDLFHSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSLLFQELEKGKKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKETLGLVETSSASPHVTHITIRRSRMLEDGYDQLRRLPVNSIKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKKVFNPALNLFKTTSGNERLYPSPTSSIHENHLQLFEFVGKMLGKAMYEGIVVDVPFASFFLSQVLGHHHSTFYSSIDELPSLDSEFYKNLTSIKRYDGDVSDLGLTLSYDEEVMGQLVCHELIPGGKTMPVTNENKISYIHLMAHFRMHTQIKEQTAAFIRGFRSIINPEWLHMFSTPEVQRLISGDNAEIDLDDLKKHTVYYGGFHSSHRVILWLWDILSSDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKSILRDKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_005165149.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165149.4

ORF Insert Sequence:

ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC 
AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC 
ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT 
AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA 
ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT 
CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA 
TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG 
TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG 
GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT 
CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA 
CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC 
GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA 
GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT 
GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT 
GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC 
TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG 
TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA 
GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA 
ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT 
CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA 
TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT 
GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG 
GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG 
CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC 
TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG 
GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC 
ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG 
CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT 
TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG 
GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG 
GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG 
CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC 
ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT 
GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG 
CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA 
GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC 
AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA 
TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG 
AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT 
TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG 
CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT 
GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT 
CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT 
ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC 
ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG 
GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC 
ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC 
CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC 
TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG 
GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC 
ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT 
CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC 
AGCATGAACA CTGGCTTTGA GCTGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3b ( XM_005165149.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165206.1 Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10273
Clone ID Related Accession (Same CDS sequence)	XM_005165149.4 , XM_005165149.3 , NM_001114682.1 , XM_005165148.3 , XM_005165148.4
Accession Version	XM_005165149.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165149.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC 
AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC 
ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT 
AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA 
ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT 
CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA 
TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG 
TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG 
GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT 
CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA 
CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC 
GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA 
GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT 
GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT 
GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC 
TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG 
TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA 
GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA 
ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT 
CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA 
TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT 
GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG 
GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG 
CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC 
TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG 
GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC 
ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG 
CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT 
TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG 
GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG 
GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG 
CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC 
ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT 
GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG 
CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA 
GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC 
AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA 
TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG 
AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT 
TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG 
CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT 
GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT 
CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT 
ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC 
ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG 
GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC 
ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC 
CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC 
TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG 
GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC 
ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT 
CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC 
AGCATGAACA CTGGCTTTGA GCTGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165206.1
CDS	294..3503
Translation	MFTATQTSKTQFLDKARVAREERKGHKEKERAATQIQALVRRFLCRCRLQKEIRKEVDDFIAVSDAGPTKRNALSIFKIARKLLFILSKDDKLRFEKLCRIILNSMDVENEPKVWYVSLALSKDLTILWLKQIKDVLWVSCEFLKKLKPDILQDNKLVTLYLTMLITFTDTSTWKIIRGKGEALKPALNRICENIMGHLNQKGFYSVLQILLTNGLARSRPSLSKGTLTAIFTLSLRPVIAAHFSDNLLRSFLLHVMSVPAFISHLNTLTPDCMITIQTHDLFRKFVLFLSREEQCLDICVCLEGSHTLCLLGNLIQLGYFNVKVLEQEAGHFVKDLTDMLSYCQRYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLVTKQLQCLWGVPVICTLFCDVLSKKLETQDPTPPPAPQPSSLQNNLPVKNLFKRAFQKSASVRNILKPVGGKRVDSAEVQKVCSICVLYQTALTTLTQIRLQILTGLTYLDELLPKLWAFICELGPQGGLRLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKMEELVTISSFLNTFVYKMVWDGILENAKGEKLDLFHSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSLLFQELEKGKKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKETLGLVETSSASPHVTHITIRRSRMLEDGYDQLRRLPVNSIKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKKVFNPALNLFKTTSGNERLYPSPTSSIHENHLQLFEFVGKMLGKAMYEGIVVDVPFASFFLSQVLGHHHSTFYSSIDELPSLDSEFYKNLTSIKRYDGDVSDLGLTLSYDEEVMGQLVCHELIPGGKTMPVTNENKISYIHLMAHFRMHTQIKEQTAAFIRGFRSIINPEWLHMFSTPEVQRLISGDNAEIDLDDLKKHTVYYGGFHSSHRVILWLWDILSSDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKSILRDKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_005165149.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165149.3

ORF Insert Sequence:

ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC 
AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC 
ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT 
AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA 
ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT 
CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA 
TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG 
TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG 
GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT 
CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA 
CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC 
GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA 
GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT 
GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT 
GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC 
TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG 
TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA 
GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA 
ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT 
CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA 
TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT 
GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG 
GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG 
CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC 
TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG 
GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC 
ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG 
CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT 
TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG 
GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG 
GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG 
CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC 
ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT 
GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG 
CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA 
GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC 
AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA 
TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG 
AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT 
TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG 
CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT 
GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT 
CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT 
ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC 
ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG 
GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC 
ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC 
CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC 
TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG 
GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC 
ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT 
CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC 
AGCATGAACA CTGGCTTTGA GCTGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3b ( XM_005165148.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165205.1 Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10273
Clone ID Related Accession (Same CDS sequence)	XM_005165149.4 , XM_005165149.3 , NM_001114682.1 , XM_005165148.3 , XM_005165148.4
Accession Version	XM_005165148.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC 
AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC 
ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT 
AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA 
ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT 
CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA 
TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG 
TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG 
GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT 
CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA 
CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC 
GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA 
GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT 
GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT 
GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC 
TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG 
TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA 
GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA 
ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT 
CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA 
TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT 
GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG 
GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG 
CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC 
TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG 
GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC 
ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG 
CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT 
TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG 
GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG 
GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG 
CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC 
ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT 
GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG 
CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA 
GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC 
AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA 
TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG 
AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT 
TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG 
CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT 
GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT 
CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT 
ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC 
ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG 
GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC 
ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC 
CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC 
TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG 
GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC 
ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT 
CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC 
AGCATGAACA CTGGCTTTGA GCTGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165205.1
CDS	208..3417
Translation	MFTATQTSKTQFLDKARVAREERKGHKEKERAATQIQALVRRFLCRCRLQKEIRKEVDDFIAVSDAGPTKRNALSIFKIARKLLFILSKDDKLRFEKLCRIILNSMDVENEPKVWYVSLALSKDLTILWLKQIKDVLWVSCEFLKKLKPDILQDNKLVTLYLTMLITFTDTSTWKIIRGKGEALKPALNRICENIMGHLNQKGFYSVLQILLTNGLARSRPSLSKGTLTAIFTLSLRPVIAAHFSDNLLRSFLLHVMSVPAFISHLNTLTPDCMITIQTHDLFRKFVLFLSREEQCLDICVCLEGSHTLCLLGNLIQLGYFNVKVLEQEAGHFVKDLTDMLSYCQRYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLVTKQLQCLWGVPVICTLFCDVLSKKLETQDPTPPPAPQPSSLQNNLPVKNLFKRAFQKSASVRNILKPVGGKRVDSAEVQKVCSICVLYQTALTTLTQIRLQILTGLTYLDELLPKLWAFICELGPQGGLRLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKMEELVTISSFLNTFVYKMVWDGILENAKGEKLDLFHSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSLLFQELEKGKKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKETLGLVETSSASPHVTHITIRRSRMLEDGYDQLRRLPVNSIKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKKVFNPALNLFKTTSGNERLYPSPTSSIHENHLQLFEFVGKMLGKAMYEGIVVDVPFASFFLSQVLGHHHSTFYSSIDELPSLDSEFYKNLTSIKRYDGDVSDLGLTLSYDEEVMGQLVCHELIPGGKTMPVTNENKISYIHLMAHFRMHTQIKEQTAAFIRGFRSIINPEWLHMFSTPEVQRLISGDNAEIDLDDLKKHTVYYGGFHSSHRVILWLWDILSSDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKSILRDKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_005165148.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165148.3

ORF Insert Sequence:

ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC 
AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC 
ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT 
AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA 
ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT 
CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA 
TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG 
TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG 
GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT 
CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA 
CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC 
GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA 
GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT 
GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT 
GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC 
TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG 
TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA 
GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA 
ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT 
CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA 
TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT 
GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG 
GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG 
CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC 
TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG 
GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC 
ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG 
CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT 
TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG 
GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG 
GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG 
CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC 
ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT 
GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG 
CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA 
GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC 
AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA 
TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG 
AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT 
TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG 
CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT 
GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT 
CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT 
ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC 
ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG 
GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC 
ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC 
CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC 
TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG 
GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC 
ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT 
CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC 
AGCATGAACA CTGGCTTTGA GCTGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3b ( XM_005165148.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165205.1 Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

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CloneID	ODa10273
Clone ID Related Accession (Same CDS sequence)	XM_005165149.4 , XM_005165149.3 , NM_001114682.1 , XM_005165148.3 , XM_005165148.4
Accession Version	XM_005165148.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165148.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC 
AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC 
ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT 
AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA 
ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT 
CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA 
TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG 
TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG 
GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT 
CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA 
CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC 
GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA 
GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT 
GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT 
GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC 
TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG 
TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA 
GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA 
ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT 
CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA 
TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT 
GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG 
GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG 
CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC 
TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG 
GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC 
ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG 
CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT 
TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG 
GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG 
GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG 
CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC 
ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT 
GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG 
CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA 
GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC 
AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA 
TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG 
AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT 
TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG 
CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT 
GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT 
CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT 
ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC 
ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG 
GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC 
ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC 
CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC 
TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG 
GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC 
ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT 
CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC 
AGCATGAACA CTGGCTTTGA GCTGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165205.1
CDS	189..3398
Translation	MFTATQTSKTQFLDKARVAREERKGHKEKERAATQIQALVRRFLCRCRLQKEIRKEVDDFIAVSDAGPTKRNALSIFKIARKLLFILSKDDKLRFEKLCRIILNSMDVENEPKVWYVSLALSKDLTILWLKQIKDVLWVSCEFLKKLKPDILQDNKLVTLYLTMLITFTDTSTWKIIRGKGEALKPALNRICENIMGHLNQKGFYSVLQILLTNGLARSRPSLSKGTLTAIFTLSLRPVIAAHFSDNLLRSFLLHVMSVPAFISHLNTLTPDCMITIQTHDLFRKFVLFLSREEQCLDICVCLEGSHTLCLLGNLIQLGYFNVKVLEQEAGHFVKDLTDMLSYCQRYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLVTKQLQCLWGVPVICTLFCDVLSKKLETQDPTPPPAPQPSSLQNNLPVKNLFKRAFQKSASVRNILKPVGGKRVDSAEVQKVCSICVLYQTALTTLTQIRLQILTGLTYLDELLPKLWAFICELGPQGGLRLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKMEELVTISSFLNTFVYKMVWDGILENAKGEKLDLFHSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSLLFQELEKGKKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKETLGLVETSSASPHVTHITIRRSRMLEDGYDQLRRLPVNSIKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKKVFNPALNLFKTTSGNERLYPSPTSSIHENHLQLFEFVGKMLGKAMYEGIVVDVPFASFFLSQVLGHHHSTFYSSIDELPSLDSEFYKNLTSIKRYDGDVSDLGLTLSYDEEVMGQLVCHELIPGGKTMPVTNENKISYIHLMAHFRMHTQIKEQTAAFIRGFRSIINPEWLHMFSTPEVQRLISGDNAEIDLDDLKKHTVYYGGFHSSHRVILWLWDILSSDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKSILRDKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_005165148.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165148.4

ORF Insert Sequence:

ATGTTCACCG CCACCCAAAC ATCCAAAACC CAGTTCCTGG ATAAAGCCAG GGTAGCACGG 
GAGGAAAGAA AAGGTCATAA GGAAAAAGAA AGAGCTGCCA CACAAATCCA GGCTCTGGTC 
AGAAGGTTTC TCTGCCGCTG CAGGCTGCAA AAAGAAATCA GGAAAGAGGT CGATGACTTC 
ATTGCAGTGA GCGATGCCGG TCCTACAAAA AGAAATGCAT TATCCATCTT TAAAATTGCT 
AGAAAGTTGC TATTTATATT GAGCAAGGAT GATAAGTTGA GGTTTGAGAA GTTGTGCAGA 
ATCATATTGA ATAGCATGGA TGTTGAGAAT GAACCTAAAG TGTGGTATGT GTCTCTGGCT 
CTTTCGAAAG ATCTCACCAT CCTCTGGCTA AAACAAATCA AAGACGTTCT TTGGGTCTCA 
TGTGAATTTC TGAAAAAACT AAAGCCTGAC ATTCTGCAAG ATAACAAATT GGTGACACTG 
TACTTGACAA TGTTGATTAC CTTCACCGAC ACATCGACAT GGAAAATCAT CCGAGGGAAG 
GGAGAAGCCC TTAAGCCTGC TTTAAACAGG ATTTGTGAGA ACATCATGGG ACATCTAAAT 
CAAAAGGGCT TCTACTCTGT TCTTCAGATT CTACTCACAA ATGGATTGGC AAGATCCAGA 
CCTTCTCTTT CCAAAGGAAC CCTCACGGCC ATTTTTACGC TGTCACTCAG GCCTGTTATC 
GCTGCACATT TCTCTGACAA CCTGCTGAGG TCTTTCCTCC TGCACGTCAT GTCTGTTCCA 
GCGTTCATCT CGCATCTCAA CACACTCACT CCAGATTGCA TGATCACCAT CCAGACCCAT 
GACCTTTTTC GCAAGTTTGT CTTGTTTTTA AGCCGAGAGG AGCAGTGTTT GGATATCTGT 
GTCTGTCTGG AGGGAAGCCA CACTCTGTGC TTGCTGGGAA ACCTCATTCA GCTCGGATAC 
TTTAATGTGA AAGTTCTAGA ACAGGAAGCA GGTCACTTTG TCAAGGATCT TACAGATATG 
TTGTCATACT GTCAGAGATA TGTGTCTCAG AAGAAATCCA ATCTCACACA TTGGCACCCA 
GTGTTGGGCT GGTTCTCCCA GACTGTTGAT TATGGGCTTA ATGAATCTAT GCCATTGGTA 
ACAAAGCAGC TGCAGTGTCT GTGGGGAGTT CCAGTGATCT GCACTCTGTT CTGTGATGTT 
CTTAGCAAAA AGCTTGAAAC TCAAGACCCC ACACCCCCAC CAGCTCCTCA GCCAAGCTCA 
TTGCAGAATA ACCTGCCCGT CAAAAATCTG TTCAAGCGAG CATTCCAGAA ATCTGCTTCT 
GTGCGGAACA TCCTGAAGCC GGTCGGGGGG AAGCGTGTGG ACTCTGCTGA GGTCCAGAAG 
GTGTGTAGTA TCTGTGTGCT GTATCAGACG GCCCTCACCA CACTCACACA AATCCGTCTG 
CAGATTCTCA CAGGACTGAC ATATCTGGAT GAATTGCTGC CTAAGCTGTG GGCATTTATC 
TGTGAGCTCG GCCCTCAGGG GGGACTCCGA CTCTTCCTGG AGTGTCTCAA TAATGACACG 
GAGGAATCCA AACAGCTCCT GGCCATGCTG ATGCTCTTCT GTGACTGCTC CAGACACCTC 
ATCACTATTC TGGATGACAT TGAGGTGTAC GAGGAACAGA TTTCTTTCAA AATGGAAGAG 
CTTGTCACGA TCTCCTCATT CCTTAACACA TTTGTATATA AGATGGTCTG GGATGGTATT 
TTAGAGAACG CCAAAGGGGA GAAGTTGGAC TTATTCCACA GTGTTCACGG CTGGCTGATG 
GTTTTGTACG AGAGGGACTG TCGCAGGAGA TTCACGCCTG AAGATCACTG GCTCCGCAAG 
GACCTGAAGC CCAGCCTACT GTTCCAAGAG CTGGAGAAAG GCAAAAAGAG AGCGCAGCTG 
CTGCTTCAGT ACATTCCTCA TGTCATCCCA CATAAAAATA GGGTGCTGCT GTTCAGGAAC 
ATAGTGACAA AAGAGAAGGA GACTCTAGGA TTGGTGGAAA CAAGCTCCGC CTCTCCTCAT 
GTGACCCATA TAACGATTCG TCGTTCACGC ATGTTGGAGG ACGGTTATGA TCAGCTGCGG 
CGTCTACCTG TCAACTCCAT TAAGGGAGTG ATCCGAGTGA AGTTTGTCAA TGATTTAGGA 
GTGGACGAGG CTGGAATTGA CCAGGATGGT GTATTTAAAG AATTCTTAGA GGAGATCATC 
AAAAAGGTTT TCAACCCAGC ACTCAATCTG TTTAAGACCA CCAGTGGGAA TGAGAGATTA 
TACCCCTCCC CAACCTCCAG CATCCATGAG AACCACCTTC AGCTGTTTGA ATTTGTGGGG 
AAGATGCTGG GAAAGGCCAT GTATGAAGGC ATTGTGGTGG ACGTGCCGTT TGCCTCTTTT 
TTCCTCAGTC AGGTTCTGGG TCACCATCAC AGCACCTTCT ATAGCTCCAT AGATGAGCTG 
CCCTCGCTCG ACTCCGAGTT TTACAAAAAC CTCACCTCCA TTAAGCGTTA TGATGGGGAT 
GTCAGTGACC TTGGTCTAAC TTTATCCTAT GATGAAGAGG TTATGGGGCA GTTAGTCTGT 
CATGAGCTCA TTCCTGGTGG AAAGACTATG CCTGTCACTA ATGAAAACAA GATCAGCTAT 
ATCCACCTGA TGGCCCATTT CCGCATGCAC ACTCAGATCA AGGAGCAGAC GGCTGCATTC 
ATCAGGGGCT TTCGCAGCAT CATCAACCCA GAGTGGCTGC ACATGTTTTC TACCCCGGAG 
GTCCAGAGGC TCATCTCGGG AGACAACGCT GAGATAGATC TGGATGATCT CAAAAAGCAC 
ACTGTGTACT ATGGAGGTTT CCATAGCAGC CACAGAGTCA TTCTTTGGCT GTGGGACATC 
CTCTCTAGTG ACTTCACCCC TGATGAGAGA GCCATGTTCC TGAAGTTTGT CACCAGCTGC 
TCAAGACCTC CTCTTCTCGG TTTTGCCTAC CTCAAACCTC CTTTCTCCAT TCGTTGTGTG 
GAAGTGTCCG ACGATCAGGA CACGGGCGAC ACTTTAGGTA GTGTTCTGCG GGGCTTCTTC 
ACCATACGTA AGAAAGAGCC CGGCGGTCGT CTCCCTACAT CCTCCACTTG TTTCAACCTT 
CTCAAGCTGC CCAACTACAG CAAGAAGAGC ATCCTCAGAG ACAAACTCCG CTACGCCATC 
AGCATGAACA CTGGCTTTGA GCTGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

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ube3b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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