fxn cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fxn
Entrez Gene ID	556389
Full Name	frataxin
Synonyms	zgc:158218
General protein information	Preferred Names frataxin Names frataxin, mitochondrial DKEYP-118A9.1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083016.1	NP_001076485.1	frataxin, mitochondrial

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FXN	NP_001074196.1
Gallus gallus (chicken)	FXN	XP_424827.3
Homo sapiens (human)	FXN	NP_000135.2
Pan troglodytes (chimpanzee)	FXN	XP_001137864.2
Danio rerio (zebrafish)	fxn	NP_001076485.1
Arabidopsis thaliana (thale cress)	FH	NP_192233.2
Xenopus tropicalis (tropical clawed frog)	LOC100497789	XP_004910856.1
	FXN	XP_001093449.1
Mus musculus (house mouse)	Fxn	NP_032070.1
Rattus norvegicus (Norway rat)	Fxn	NP_001178881.1
Canis lupus familiaris (dog)	FXN	NP_001103428.1
Oryza sativa (rice)	Os01g0783600	NP_001044455.2

Gene Ontology
Bibliography

Related articles in PubMed

FusX: A Rapid One-Step Transcription Activator-Like Effector Assembly System for Genome Science.
Ma AC, McNulty MS, Poshusta TL, Campbell JM, Mart?nez-G?lvez G, Argue DP, Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker SC
Human gene therapy27(6)451-63(2016 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Two functional microRNA-126s repress a novel target gene p21-activated kinase 1 to regulate vascular integrity in zebrafish.
Zou J, Li WQ, Li Q, Li XQ, Zhang JT, Liu GQ, Chen J, Qiu XX, Tian FJ, Wang ZZ, Zhu N, Qin YW, Shen B, Liu TX, Jing Q
Circulation research108(2)201-9(2011 Jan)

Consequences of lineage-specific gene loss on functional evolution of surviving paralogs: ALDH1A and retinoic acid signaling in vertebrate genomes.
Ca?estro C, Catchen JM, Rodr?guez-Mar? A, Yokoi H, Postlethwait JH
PLoS genetics5(5)e1000496(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fxn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fxn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36691	NM_001083016.1 Latest version!	Danio rerio frataxin (fxn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fxn ( NM_001083016.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001076485.1 Danio rerio frataxin (fxn), mRNA.

Price & Availability↑

CloneID	ODa36691
Clone ID Related Accession (Same CDS sequence)	NM_001083016.1
Accession Version	NM_001083016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Danio rerio(zebrafish)
Product	frataxin, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC131866.1. On Nov 16, 2007 this sequence version replaced XM_679179.2. ##Evidence-Data-START## Transcript exon combination :: BC131866.1, GW712779.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAGCAGTG GACTTAATTC AATAAGTGGT GGTAGGAGTG TTTCTTCAGC CAATATATGT 
GGAAGACATA CACAGTGCTT TGATAGGATT CTAAACAAAA GAGACCTTCA TTTAAGCGGT 
CCGCTTGGAG AAGAAAAGGC ACATCACCTC AGAGAAATAT CAGAGGCCGA GTATGAGAGA 
CTAGCAGAAG AGACGCTGGA TGCATTAGCA GATTATTTTG AAGATCTCAC AGATGAAAAC 
TTCACTGGAC TGGACTATGA CGTCGTCTTT TCTAATGGAG TGCTGACAGT GAAAGTGGGC 
TCAGACCATG GGACATACGT TATCAACAAG CAGACCCCCA ACCGACAGAT ATGGCTCTCA 
TCGCCGACAA GTGGGCCAAA GCGTTACGAT TGGACGGGCG AGCGATGGGT TTACACACAC 
GATGCAGTGC CATTGCATAG TCTGCTCTCC AAAGAGTTGT CTATCATCTT CAAAACAAAT 
ATAGACCTGT CGCATCTCAT CCATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076485.1
CDS	134..643
Translation	MSSGLNSISGGRSVSSANICGRHTQCFDRILNKRDLHLSGPLGEEKAHHLREISEAEYERLAEETLDALADYFEDLTDENFTGLDYDVVFSNGVLTVKVGSDHGTYVINKQTPNRQIWLSSPTSGPKRYDWTGERWVYTHDAVPLHSLLSKELSIIFKTNIDLSHLIHS

Target ORF information:

RefSeq Version	NM_001083016.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio frataxin (fxn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083016.1

ORF Insert Sequence:

ATGAGCAGTG GACTTAATTC AATAAGTGGT GGTAGGAGTG TTTCTTCAGC CAATATATGT 
GGAAGACATA CACAGTGCTT TGATAGGATT CTAAACAAAA GAGACCTTCA TTTAAGCGGT 
CCGCTTGGAG AAGAAAAGGC ACATCACCTC AGAGAAATAT CAGAGGCCGA GTATGAGAGA 
CTAGCAGAAG AGACGCTGGA TGCATTAGCA GATTATTTTG AAGATCTCAC AGATGAAAAC 
TTCACTGGAC TGGACTATGA CGTCGTCTTT TCTAATGGAG TGCTGACAGT GAAAGTGGGC 
TCAGACCATG GGACATACGT TATCAACAAG CAGACCCCCA ACCGACAGAT ATGGCTCTCA 
TCGCCGACAA GTGGGCCAAA GCGTTACGAT TGGACGGGCG AGCGATGGGT TTACACACAC 
GATGCAGTGC CATTGCATAG TCTGCTCTCC AAAGAGTTGT CTATCATCTT CAAAACAAAT 
ATAGACCTGT CGCATCTCAT CCATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FusX: A Rapid One-Step Transcription Activator-Like Effector Assembly System for Genome Science.
Human gene therapy27(6)451-63(2016 Jun)
Ma AC,McNulty MS,Poshusta TL,Campbell JM,Mart?nez-G?lvez G,Argue DP,Lee HB,Urban MD,Bullard CE,Blackburn PR,Man TK,Clark KJ,Ekker SC

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Two functional microRNA-126s repress a novel target gene p21-activated kinase 1 to regulate vascular integrity in zebrafish.
Circulation research108(2)201-9(2011 Jan)
Zou J,Li WQ,Li Q,Li XQ,Zhang JT,Liu GQ,Chen J,Qiu XX,Tian FJ,Wang ZZ,Zhu N,Qin YW,Shen B,Liu TX,Jing Q

Consequences of lineage-specific gene loss on functional evolution of surviving paralogs: ALDH1A and retinoic acid signaling in vertebrate genomes.
PLoS genetics5(5)e1000496(2009 May)
Ca?estro C,Catchen JM,Rodr?guez-Mar? A,Yokoi H,Postlethwait JH