ca4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ca4a
Entrez Gene ID	555196
Full Name	carbonic anhydrase IV a
Synonyms	zgc:171842
General protein information	Preferred Names carbonic anhydrase IV a Names carbonic anhydrase IV a zCA4a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001114407.1	NP_001107879.1	carbonic anhydrase IV a precursor
NM_001114407.2	NP_001107879.2	carbonic anhydrase IV a precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ca4a	NP_001107879.1
Xenopus tropicalis (tropical clawed frog)	ca4	XP_002933882.2
Pan troglodytes (chimpanzee)	CA4	XP_001140977.1
Danio rerio (zebrafish)	ca4c	XP_003200928.1
Homo sapiens (human)	CA4	NP_000708.1
Bos taurus (cattle)	CA4	NP_776322.1
Gallus gallus (chicken)	CA4	XP_415893.1
Canis lupus familiaris (dog)	CA4	XP_537711.3
Mus musculus (house mouse)	Car4	NP_031633.1
Danio rerio (zebrafish)	ca4b	NP_001159683.1
	CA4	XP_001107970.2
Rattus norvegicus (Norway rat)	Car4	NP_062047.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Identification and expression of a novel carbonic anhydrase isozyme in the pufferfish Takifugu vermicularis.
Sumi KR, Nou IS, Kho KH
Gene588(2)173-9(2016 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Chemokine-Dependent pH Elevation at the Cell Front Sustains Polarity in Directionally Migrating Zebrafish Germ Cells.
Tarbashevich K, Reichman-Fried M, Grimaldi C, Raz E
Current biology : CB25(8)1096-103(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ca4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ca4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa05176	NM_001114407.1 Latest version!	Danio rerio carbonic anhydrase IV a (ca4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa159842	NM_001114407.2 Latest version!	Danio rerio carbonic anhydrase IV a (ca4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ca4a ( NM_001114407.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001107879.1 Danio rerio carbonic anhydrase IV a (ca4a), mRNA.

Price & Availability↑

CloneID	ODa05176
Clone ID Related Accession (Same CDS sequence)	NM_001114407.1
Accession Version	NM_001114407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	carbonic anhydrase IV a precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC155085.1. On Feb 10, 2008 this sequence version replaced XM_677649.2. ##Evidence-Data-START## Transcript exon combination :: BC155085.1 [ECO:0000332] ##Evidence-Data-END##

ATGCGGTGTC TGCTTTCAGC TTATCTTCTG CCTCTGATTC TGCATGCATG CACCGGTGCA 
GACTGGTGCT ACCAGACACA AGTGAGCTGT GATTCCCACT GTAAAGGTCC AGAGAAATGG 
AGAGAAGTCA ATGCAGACTG CGGGAAGGAC AGACAGTCAC CCATAAACAT AGTGACCAAG 
CAAACCAAGC TGGACGAGCG ATTGACACCT TTCAGATTCA CTGGATACCA GACCGTTTTC 
GATGGCACAT TGAAGAACAA TGGACACTCG GTTCAGGTCA GTATTCCTGT CCCAGCCACT 
ATTTCTGGAG GAAATCTGGC AGAACCATAT AAAGCTGTGC AGTTTCACCT GCACTGGGGC 
ATCAGCAGCG GCCCAGGCTC TGAACACACC ATTGATGGAG AGCAGTATCC AATGGAGCTG 
CATATTGTGC ACATGAAGCA GAAATACATC CGGATTGAAG ACGCTCTCAA AGACCCGTCT 
GGAGTGGCTG TGCTGGGATT CTTCTATGAG GTGTCCAGCA CCACAAACAG AAAATATGAC 
CTGTTCGCAC ACGCACTGCG CAGCGTACAG AACACAAATG GCAACACGAC TCTTCGTAAG 
ATCTCTCTGA ATCAGCTCAT CCTGCCTGAA GTGAACATGA CCAATTATTA CCGGTATGAC 
GGGTCACTGA CCACACCGGG ATGCACCGAG GCTGTGGTGT GGACGGTGTT TGAAAAGCCC 
ATTCCCCTGG ACAGTGAACA GCTTCAGGCT TTCTCCTCTC TGAAGTTTCG CGATGGGAAG 
CCGATGGTCG GAACGTTCAG ACCCGTTCAG CCTCGTAACG GGCGTGTGGT TTATCGGTCC 
AGCGGTGGGA CTTTATTGGT CAGTGTTTTC CTTCTGATGG CTTCCATCAG CACAGCATTC 
ACCATAACCA GACTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107879.1
CDS	34..954
Translation	MRCLLSAYLLPLILHACTGADWCYQTQVSCDSHCKGPEKWREVNADCGKDRQSPINIVTKQTKLDERLTPFRFTGYQTVFDGTLKNNGHSVQVSIPVPATISGGNLAEPYKAVQFHLHWGISSGPGSEHTIDGEQYPMELHIVHMKQKYIRIEDALKDPSGVAVLGFFYEVSSTTNRKYDLFAHALRSVQNTNGNTTLRKISLNQLILPEVNMTNYYRYDGSLTTPGCTEAVVWTVFEKPIPLDSEQLQAFSSLKFRDGKPMVGTFRPVQPRNGRVVYRSSGGTLLVSVFLLMASISTAFTITRLN

Target ORF information:

RefSeq Version	NM_001114407.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carbonic anhydrase IV a (ca4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114407.1

ORF Insert Sequence:

ATGCGGTGTC TGCTTTCAGC TTATCTTCTG CCTCTGATTC TGCATGCATG CACCGGTGCA 
GACTGGTGCT ACCAGACACA AGTGAGCTGT GATTCCCACT GTAAAGGTCC AGAGAAATGG 
AGAGAAGTCA ATGCAGACTG CGGGAAGGAC AGACAGTCAC CCATAAACAT AGTGACCAAG 
CAAACCAAGC TGGACGAGCG ATTGACACCT TTCAGATTCA CTGGATACCA GACCGTTTTC 
GATGGCACAT TGAAGAACAA TGGACACTCG GTTCAGGTCA GTATTCCTGT CCCAGCCACT 
ATTTCTGGAG GAAATCTGGC AGAACCATAT AAAGCTGTGC AGTTTCACCT GCACTGGGGC 
ATCAGCAGCG GCCCAGGCTC TGAACACACC ATTGATGGAG AGCAGTATCC AATGGAGCTG 
CATATTGTGC ACATGAAGCA GAAATACATC CGGATTGAAG ACGCTCTCAA AGACCCGTCT 
GGAGTGGCTG TGCTGGGATT CTTCTATGAG GTGTCCAGCA CCACAAACAG AAAATATGAC 
CTGTTCGCAC ACGCACTGCG CAGCGTACAG AACACAAATG GCAACACGAC TCTTCGTAAG 
ATCTCTCTGA ATCAGCTCAT CCTGCCTGAA GTGAACATGA CCAATTATTA CCGGTATGAC 
GGGTCACTGA CCACACCGGG ATGCACCGAG GCTGTGGTGT GGACGGTGTT TGAAAAGCCC 
ATTCCCCTGG ACAGTGAACA GCTTCAGGCT TTCTCCTCTC TGAAGTTTCG CGATGGGAAG 
CCGATGGTCG GAACGTTCAG ACCCGTTCAG CCTCGTAACG GGCGTGTGGT TTATCGGTCC 
AGCGGTGGGA CTTTATTGGT CAGTGTTTTC CTTCTGATGG CTTCCATCAG CACAGCATTC 
ACCATAACCA GACTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ca4a ( NM_001114407.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001107879.2 Danio rerio carbonic anhydrase IV a (ca4a), mRNA.

Price & Availability↑

CloneID	ODa159842
Clone ID Related Accession (Same CDS sequence)	NM_001114407.2
Accession Version	NM_001114407.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	carbonic anhydrase IV a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU694217.4 and FO680663.4. On Apr 20, 2018 this sequence version replaced NM_001114407.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC155085.1, GFIL01002775.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505370, SAMEA3505371 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGTGTC TGCTTTCAGC TTATCTTCTG CCTCTGATTC TGCATGCATG CACCGGTGCA 
GACTGGTGCT ACCAGACACA AGTGAGCTGT GATTCCCACT GTAAAGGTCC AGAGAAATGG 
AGAGAAGTCA ATGCAGACTG CGGGAAGGAC AGACAGTCAC CCATAAACAT AGTGACCAAG 
CAAACCAAGC TGGACGAGCG ATTGACACCT TTCAGATTCA CTGGATACCA GACCGTGTTC 
GATGGCACAT TGAAGAACAA TGGACACTCG GTTCAGGTCA GTATTCCTGT CCCAGCCACT 
ATTTCTGGAG GAAATCTGGC AGAACCATAT AAAGCTGTGC AGTTTCACCT GCACTGGGGC 
ATCAGCAGCG GCCCAGGCTC TGAACACACC ATTGATGGAG AGCAGTATCC AATGGAGCTG 
CACATTGTGC ACATGAAGCA GAAATACATC CGGATTGAAG ACGCTCTCAA AGACCCGTCT 
GGAGTGGCTG TGCTGGGATT TTTCTATGAG GTGTCCAGCA CCACAAACAG AAAATATGAC 
CTGTTCGCAC ACGCACTGCG CAGCGTACAG AACACAAATG GCAACACGAC TCTTCGTAAG 
ATCTCTCTGA ATCAGCTCAT CCTGCCTGAA GTGAACATGA CCAATTATTA CCGGTATGAC 
GGGTCACTGA CCACACCGGG ATGCACCGAG GCTGTGGTGT GGACGGTGTT TGAAAAGCCC 
ATTCCCCTGG ACAGTGAACA GCTTCAGGCT TTCTCCTCTC TGAAGTTTCG CGATGGGAAG 
CCGATGGTCG GAACGTTCAG ACCCGTTCAG CCTCGTAACG GGCGAGTGGT TTATCGGTCC 
AGCGGTGGGA CTTTATTGGT CAGTGTTTTC CTTCTGATGG CTTCCGTCAG CACAGCATTC 
ACCATAACCA GACTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107879.2
CDS	34..954
Translation	MRCLLSAYLLPLILHACTGADWCYQTQVSCDSHCKGPEKWREVNADCGKDRQSPINIVTKQTKLDERLTPFRFTGYQTVFDGTLKNNGHSVQVSIPVPATISGGNLAEPYKAVQFHLHWGISSGPGSEHTIDGEQYPMELHIVHMKQKYIRIEDALKDPSGVAVLGFFYEVSSTTNRKYDLFAHALRSVQNTNGNTTLRKISLNQLILPEVNMTNYYRYDGSLTTPGCTEAVVWTVFEKPIPLDSEQLQAFSSLKFRDGKPMVGTFRPVQPRNGRVVYRSSGGTLLVSVFLLMASVSTAFTITRLN

Target ORF information:

RefSeq Version	NM_001114407.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carbonic anhydrase IV a (ca4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114407.2

ORF Insert Sequence:

ATGCGGTGTC TGCTTTCAGC TTATCTTCTG CCTCTGATTC TGCATGCATG CACCGGTGCA 
GACTGGTGCT ACCAGACACA AGTGAGCTGT GATTCCCACT GTAAAGGTCC AGAGAAATGG 
AGAGAAGTCA ATGCAGACTG CGGGAAGGAC AGACAGTCAC CCATAAACAT AGTGACCAAG 
CAAACCAAGC TGGACGAGCG ATTGACACCT TTCAGATTCA CTGGATACCA GACCGTGTTC 
GATGGCACAT TGAAGAACAA TGGACACTCG GTTCAGGTCA GTATTCCTGT CCCAGCCACT 
ATTTCTGGAG GAAATCTGGC AGAACCATAT AAAGCTGTGC AGTTTCACCT GCACTGGGGC 
ATCAGCAGCG GCCCAGGCTC TGAACACACC ATTGATGGAG AGCAGTATCC AATGGAGCTG 
CACATTGTGC ACATGAAGCA GAAATACATC CGGATTGAAG ACGCTCTCAA AGACCCGTCT 
GGAGTGGCTG TGCTGGGATT TTTCTATGAG GTGTCCAGCA CCACAAACAG AAAATATGAC 
CTGTTCGCAC ACGCACTGCG CAGCGTACAG AACACAAATG GCAACACGAC TCTTCGTAAG 
ATCTCTCTGA ATCAGCTCAT CCTGCCTGAA GTGAACATGA CCAATTATTA CCGGTATGAC 
GGGTCACTGA CCACACCGGG ATGCACCGAG GCTGTGGTGT GGACGGTGTT TGAAAAGCCC 
ATTCCCCTGG ACAGTGAACA GCTTCAGGCT TTCTCCTCTC TGAAGTTTCG CGATGGGAAG 
CCGATGGTCG GAACGTTCAG ACCCGTTCAG CCTCGTAACG GGCGAGTGGT TTATCGGTCC 
AGCGGTGGGA CTTTATTGGT CAGTGTTTTC CTTCTGATGG CTTCCGTCAG CACAGCATTC 
ACCATAACCA GACTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Identification and expression of a novel carbonic anhydrase isozyme in the pufferfish Takifugu vermicularis.
Gene588(2)173-9(2016 Aug)
Sumi KR,Nou IS,Kho KH

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Chemokine-Dependent pH Elevation at the Cell Front Sustains Polarity in Directionally Migrating Zebrafish Germ Cells.
Current biology : CB25(8)1096-103(2015 Apr)
Tarbashevich K,Reichman-Fried M,Grimaldi C,Raz E