nalcn cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nalcn
Entrez Gene ID	548345
Full Name	sodium leak channel, non-selective
Synonyms	zgc:113349
General protein information	Preferred Names sodium leak channel, non-selective Names sodium leak channel non-selective protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005167698.3	XP_005167755.1	sodium leak channel non-selective protein isoform X1
XM_009304740.2	XP_009303015.1	sodium leak channel non-selective protein isoform X2
XM_009304739.2	XP_009303014.1	sodium leak channel non-selective protein isoform X2
NM_001017549.2	NP_001017549.2	sodium leak channel non-selective protein precursor
XM_017357669.1	XP_017213158.1	sodium leak channel non-selective protein isoform X1
XM_005167698.4	XP_005167755.1	sodium leak channel non-selective protein isoform X1
XM_009304740.3	XP_009303015.1	sodium leak channel non-selective protein isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NALCN	XP_001149573.1
Canis lupus familiaris (dog)	NALCN	XP_542654.4
Bos taurus (cattle)	NALCN	XP_002692026.1
Rattus norvegicus (Norway rat)	Nalcn	NP_705894.1
Drosophila melanogaster (fruit fly)	na	NP_001096981.1
Homo sapiens (human)	NALCN	NP_443099.1
Caenorhabditis elegans (roundworm)	nca-2	NP_498054.2
Mus musculus (house mouse)	Nalcn	NP_796367.3
Caenorhabditis elegans (roundworm)	unc-77	NP_741413.2
Gallus gallus (chicken)	NALCN	XP_416967.2
Danio rerio (zebrafish)	nalcn	NP_001017549.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003921	XP_318373.5
Xenopus tropicalis (tropical clawed frog)	nalcn	XP_002937125.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nalcn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nalcn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00404	NM_001017549.2 Latest version!	Danio rerio sodium leak channel, non-selective (nalcn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa15242	XM_005167698.4 Latest version!	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa15242	XM_017357669.1 Latest version!	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41705	XM_009304740.3 Latest version!	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41705	XM_009304739.2 Latest version!	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa15242	XM_005167698.3	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41705	XM_009304740.2	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nalcn ( NM_001017549.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017549.2 Danio rerio sodium leak channel, non-selective (nalcn), mRNA.

Price & Availability↑

CloneID	ODa00404
Clone ID Related Accession (Same CDS sequence)	NM_001017549.2
Accession Version	NM_001017549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	sodium leak channel non-selective protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX571786.6 and BX571700.8. On Aug 11, 2010 this sequence version replaced NM_001017549.1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript and protein alignments, including mouse NP_796367.3, human NP_443099.1, and RNA-Seq data. ##Evidence-Data-START## CDS exon combination :: GFIL01027617.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGCAGTG CTGGAGCAAT TTCAGCTTTA CTTTTCCCGT TGATCCACTC TCTCTATAGC 
ACAGCGAGCC CAAGCATGCT GAAGAGGAAG CAGAGCTCGC GGGTCGAGGC CCAGCCTATG 
ACTGACTTTG GGCCAGACGA AACACTGACA GACAGTGCTG ACATTCTTTG GATCAATAAA 
CCATGGGTTC ACTCTCTCCT GCGGGCCTGT GCCATAATCA GTGTTATTTC GGTTTGTATG 
AATACACCTA AGACCTTTGA GCACTACCCT CCCCTACAGT ATGTCACCTT CGCCCTGGAC 
ACACTGCTGA TGTTCCTGTA CACCGCAGAG ATGATCGCCA AAATGCATAT CAGGGGCATC 
ATCAAGGGAG AAAACTCCTA TGTTAAAGAC CGCTGGTGCA TGTTTGATGG CTTCATGGTG 
GTCTTCCTCT GGGTCTCTCT TGTGCTTCAG GTGTTTGAAA TAGCGGAGCT CGTGGACCAA 
ATGTCACCCT GGGGAATGTT ACGCATACCG AGGGCCCTTA TTATGATCCG AGTCTTCCGA 
ATCTATTTCC GATTTGAATT GCCACGTAGC CGGATAACCA ACATTCTCAA GCGCTCAGGG 
GAGCAGATCT GGAGTGTGAC CATATTTTTG CTATTCTTCC TTCTTCTTTA TGGAATCCTG 
GGGGTTCAGA TGTTTGGCAC ATTCAATCAT CATTGCGTCA CCAATGAAAC AGAAAAAGAG 
AAAGTGACAT GGAACAGCCT AGCAATCCCG GATACACACT GCTCTCCGAA CGGGGAGGGA 
TATCAGTGTC CAGTGGGCTT TAAGTGTGTG GATCTGGAAG ATTATGGACT CAGTAGACAA 
GAACTTGGAT ACAGTGGATT CAATGAGCTT GGCACCAGTA TATTCACTGT ATATGAGGCT 
GCGTCTCAGG AGGGCTGGGT CTTCATCATG TATCGAGCCA TCGACAGCTT CCCACGCTGG 
CGCTCATATT TCTACTTCCT CACGCTCATC TTCTTCCTCG CCTGGCTGGT CAAGAATGTG 
TTCATTGCTG TGATTATCGA AACGTTTGCT GAGATCAGAG TGCAGTTCCA GCAGATGTGG 
GGATCCCGGA GCAGCACCAC GTCAACAGCT ACTACACAGA TGTTTCACGA GGACGCGTCT 
GGAGGATGGC AGCTCGTGGC GGTGGACGTG AATAAACCAC ATGGCCGGGC GCCTGCTTGC 
CTCCAGCAAC TGATGCGATC TTCAGTTTTC CACATGTTCA TCCTCAGTAT GGTGGCTGTT 
GATGTGATTG TAGCTGCGAG CAATTACCAC AGAGGTGACC ATAAACTCAC CAATGATGAG 
TTCTACCTGG CTGAGGTCGC CTTCACTGTG CTGTTTGATT TGGAGGCTCT GCTGAAAATC 
TGGTGTTTGG GCTTCACTGG ATACATCAGT TCATCGCTGC ACAAGTTTGA GTCGCTGCTT 
GTAGTGGGCA CCACCTTGCA CATCTACCCT GACCTTTACC ACTCGCAGTT CACCTACTTT 
CAGGTTTTGC GAGTGGTCCG TCTAATAAAA ATCTCCCCTG CACTAGAGGA CTTTGTGTAC 
AAGATCTTTG GTCCAGGGAA GAAACTGGGC AGTCTAGTGG TGTTTACTGC CAGTCTGCTC 
ATAGTGATGT CTGCCATCAG TCTGCAGATG TTCTGCTTTG TGGAGGATTT GGATCGTTTC 
ACCACATTTC CACGGGCGTT TATGTCCATG TTCCAGATTC TCACGCAGGA GGGCTGGGTG 
GATGTCATGG ACCAAACACT GGTGGCTGTG GGACAGATGT GGGCTCCGGT GGTGGCCATA 
TACTTTATTC TGTATCACCT TTTTGCCACA CTGATCCTTC TGAGTCTTTT TGTTGCTGTT 
ATTTTGGACA ATCTTGAGTT GGATGAAGAC CTGAAAAAGT TAAAACAGCT GAAGCAGAGT 
GAAGCCAATG CTGATACAAA AGAGAAACTT CCTTTGCGAC TGCGAATCTT TGAGAAGTTC 
CCCAATCGAC CGCAGATGGT GAAAATCTCC AAGCTGCCTT CAGACTTCAC TGTGCCCAGA 
ATCAGGGAGA GTTTCATGAA GCAGTTCATC GACCGACAGC AGCAGGACCC TAGCTGTCTG 
TTCCGTCGCC TTCCCTCAGC ATCATCCTCG TCTTGCGACC ATTCCAAACG CTCTGCCATT 
GAGGATAATA AATACATAGA TCAGAAGCTA CGAAAGTCAG TATTTAGCAT CCGGGCTCGC 
AACCTAATGG AAAAGGAAAT CACTGTCAAC AAAATTTTGC GAGCCTGCAC CAGGCAGCGC 
ATGCTGAGTG GCTCATTTGA GGGTCAGCCA ACTAAAGAGA GATCCATTCT CAGTGTCCAG 
CATCACATCC GCCAAGAACG CCGGTCCCTC AGACATGGCT CTACCAGCCA GAGAATCAGC 
AGAGGGAAAT CCTTGGAAAC ACTCACTCAG GACCACTCCA GCACGGTCCG CTACCGTAAT 
GCCCAGCGGG AGGACAGTGA AATCAAGATG ATACAAGAGA AGAAGGAGCA GGCTGAGATG 
AAACGAAAAG TCCAAGAGGA GGAACTTCGG GAAAACCATC CATACTTTGA CAAGCCACTC 
TTCATCGTTG GTAGAGAGCA TCGATTCAGA AACTTTTGCC GAATGATTGT TAGAGCCCGT 
TTTAATGCGT CAAATACGGA TCCCAATACA GGCGCAGTGA ACAGCACAAA ATACCATCAA 
TTATATGATT TGCTGGGCTT GGTAACCTAT CTGGACTGGG TGATGATCGT GGTCACTATC 
TGCTCTTGTA TTTCCATGAT GTTTGAGTCT CCTTTTACTA GAGTCATGCA TGTCCCAACC 
CTGCAGATTG GAGAATATGT GTTCGTCATC TTTATGAGCA TTGAGCTGAA CCTGAAGATT 
ATGGCAGATG GCCTGTTCTT CACTCCCACT GCAGTCATCC GAGACTTTGG CGGCGTCATG 
GACATCTTCA TCTACTTAGT AAGTTTGATC TTTTTATGCT GGCTGCCAAA TAATGTGCCC 
CCTGAGTCTG GTGCCCAGCT CCTCATGATG TTACGTTGCT TCCGGCCACT GAGAATCTTT 
AAACTGGTGC CCCAAATGAG GAAGGTGGTT CGAGAGGTCC TCAAGGGTTT CAAAGAGATT 
TTTCTGGTCT CTATTCTTCT CCTGACACTA ATGCTGGTGT TCGCAACCTT CGGGGTGCAA 
CTCTTTGCTG GAAAACTTGC CAAATGTAAT GATCCTCATA TTTCTAGCAA GGATGACTGT 
CACGGCATCT TTAGAATCAA TGTGAGCATT TCAAAGAACC TGAATTTGAA GCTCAAGGAT 
GGGGAGAAGA AACCAGGATT TTGGGTGCCA AGAGTTTGGG CAAACCCTCG AAACTTCAAT 
TTTGATAATG TGGGCAATGC CATGCTGGCT CTGTTTGAAG TGTTGTCGCT GAAGGGTTGG 
GTGGAGGTGA GAGATGTCAT CATTCACAGA GTAGGACCGA TTCATGGTAT CTACATCCAT 
GTCTTTGTGT TCTTGGGATG TATGATCGGA CTCACCCTTT TTGTTGGCGT TGTCATTGCC 
AACTTTAATG AAAATAAGGG CACTGCTCTG TTGACTGTGG ATCAGAGGAG ATGGGAAGAT 
TTAAAGAGTC GACTCAAGAT TGCTCAGCCC CTCCACCTCC CTCCACGTCC AGAAAATGGT 
GGATTTCGTG CAAAGATGTA TGACATCACC CAACATCCCT TTTTCAAGCG AGGGATTGCT 
GTGCTGGTTT TAGCACAGTC TGTCCTCCTG TCGGTTAAGT GGGATGAGCT TGGCGCAGTT 
ACGTTTCCCT TGGCCACAAT GTCAGTGGTT TTCACTTTCA TTTTTGTTTT GGAGGTGACT 
ATGAAGCTGA TAGCCATGTC ACCAGCTGGA TACTGGCAGA GCAGGAGGAA CAGATATGAC 
CTACTCGTCA CATCCCTCGG GCTCATATGG ATCATTCTGC ACTTCGCTTT ACAGAATGCT 
TACACATACA TGATGGGCAC CTGTGTGATA GTATTCAGAT TTTTCACCAT ATGCGGAAAG 
CATGTGACGT TAAAGATGCT TCTGCTTACT GTGGTCGTCA GTATGTACAA GAGTTTCTTC 
ATTATCGTGG GCATGTTTTT GCTGCTGCTG TGCTACGCTT TCGCCGGCGT CGTTCTTTTT 
GGCACCGTCA AATACGGAGA GAACATTAAC AGACACGCAA ACTTCTCCAC CGCAGGGAAA 
GCCATCACCG TACTTATTCG AATAGTCACA GGTGAAGACT GGAATAAGAT CATGCATGAC 
TGCATGGTGC AGCCGCCGTT CTGCACCCCA GACAAACATC GCTACTGGGA GACGGACTGC 
GGCAACTACG CTGGAGCCCT CATTTACTTC TGCTCCTTCT ATGTCATCAT CGCTTACATC 
ATGCTCAACC TGCTCGTAGC CATCATTGTG GAGAATTTCT CTTTGTTCTA CTCCACCGAA 
GAGGACCAGT TGCTAAGCTA CAACGACCTG CGTCACTTCC AGATCATCTG GAACATGGTG 
GATGACAAGC GGGAGGGAGT GATTCCGACC TCTCGGGTGA AGTTTCTGCT CCGTCTGCTG 
AGAGGTCGTC TGGAAGTGGA TCTGGACAAA GACAAGCTTC TCTTTAAACA CATGTGTTAT 
GAGATGGAGC GTCTGCACAA TGGAGGAGAC GTCACCTTTC ATGATGTACT GAGCATGCTG 
TCGTATCGTT CAGTTGATAT CCGCAAGAGT CTTCAGCTGG AGGAGCTGTT GGCAAGAGAG 
CAGCTTGAAT ACACTATAGA GGAGGAAGTG GCCAAACAAA CCATCCGCAT GTGGTTGAAG 
AAGTGCCTGA AACGCATCAG AGCCAAACAG CAGCAGTCAT GCAGCATCAT CCTCAGTCTG 
AGAGAGAGTC AACAGCAAGA CCTGCGGCGG CTGCTCAACC CCCCCAGCAT CGAGACCACC 
GTCCCCAGCG AAGACACCAA CACACACAAC CAGGACAATC CCACCCAGCC TGAGAACAGC 
GGCCTGCAGA CGTTACTGAG CCCCACTCTG TCAGACCGCA GTGGATACAG ACAGGACTCT 
GCTGACCGAC CCCAGAGGAA GCTGGGACAG TGGAGACTTC CTGCAGGCCG CACAAGCGTG 
AAGTCCATGG TGTGTAAGAT GAACCCGGTG ACTGACGAGG CGTCCTCAGG CTCTGAAGTT 
AAGAAGTGGT GGACGCGTCA ACTGACTGTG GAAAGTGATG AAAGTGGAGA CGACCTCATT 
GATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017549.2
CDS	206..5434
Translation	MCSAGAISALLFPLIHSLYSTASPSMLKRKQSSRVEAQPMTDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIKGENSYVKDRWCMFDGFMVVFLWVSLVLQVFEIAELVDQMSPWGMLRIPRALIMIRVFRIYFRFELPRSRITNILKRSGEQIWSVTIFLLFFLLLYGILGVQMFGTFNHHCVTNETEKEKVTWNSLAIPDTHCSPNGEGYQCPVGFKCVDLEDYGLSRQELGYSGFNELGTSIFTVYEAASQEGWVFIMYRAIDSFPRWRSYFYFLTLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDASGGWQLVAVDVNKPHGRAPACLQQLMRSSVFHMFILSMVAVDVIVAASNYHRGDHKLTNDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFESLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEDLDRFTTFPRAFMSMFQILTQEGWVDVMDQTLVAVGQMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPRIRESFMKQFIDRQQQDPSCLFRRLPSASSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLMEKEITVNKILRACTRQRMLSGSFEGQPTKERSILSVQHHIRQERRSLRHGSTSQRISRGKSLETLTQDHSSTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRMIVRARFNASNTDPNTGAVNSTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFTRVMHVPTLQIGEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWLPNNVPPESGAQLLMMLRCFRPLRIFKLVPQMRKVVREVLKGFKEIFLVSILLLTLMLVFATFGVQLFAGKLAKCNDPHISSKDDCHGIFRINVSISKNLNLKLKDGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPENGGFRAKMYDITQHPFFKRGIAVLVLAQSVLLSVKWDELGAVTFPLATMSVVFTFIFVLEVTMKLIAMSPAGYWQSRRNRYDLLVTSLGLIWIILHFALQNAYTYMMGTCVIVFRFFTICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLIRIVTGEDWNKIMHDCMVQPPFCTPDKHRYWETDCGNYAGALIYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTSRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIILSLRESQQQDLRRLLNPPSIETTVPSEDTNTHNQDNPTQPENSGLQTLLSPTLSDRSGYRQDSADRPQRKLGQWRLPAGRTSVKSMVCKMNPVTDEASSGSEVKKWWTRQLTVESDESGDDLIDI

Target ORF information:

RefSeq Version	NM_001017549.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sodium leak channel, non-selective (nalcn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017549.2

ORF Insert Sequence:

ATGTGCAGTG CTGGAGCAAT TTCAGCTTTA CTTTTCCCGT TGATCCACTC TCTCTATAGC 
ACAGCGAGCC CAAGCATGCT GAAGAGGAAG CAGAGCTCGC GGGTCGAGGC CCAGCCTATG 
ACTGACTTTG GGCCAGACGA AACACTGACA GACAGTGCTG ACATTCTTTG GATCAATAAA 
CCATGGGTTC ACTCTCTCCT GCGGGCCTGT GCCATAATCA GTGTTATTTC GGTTTGTATG 
AATACACCTA AGACCTTTGA GCACTACCCT CCCCTACAGT ATGTCACCTT CGCCCTGGAC 
ACACTGCTGA TGTTCCTGTA CACCGCAGAG ATGATCGCCA AAATGCATAT CAGGGGCATC 
ATCAAGGGAG AAAACTCCTA TGTTAAAGAC CGCTGGTGCA TGTTTGATGG CTTCATGGTG 
GTCTTCCTCT GGGTCTCTCT TGTGCTTCAG GTGTTTGAAA TAGCGGAGCT CGTGGACCAA 
ATGTCACCCT GGGGAATGTT ACGCATACCG AGGGCCCTTA TTATGATCCG AGTCTTCCGA 
ATCTATTTCC GATTTGAATT GCCACGTAGC CGGATAACCA ACATTCTCAA GCGCTCAGGG 
GAGCAGATCT GGAGTGTGAC CATATTTTTG CTATTCTTCC TTCTTCTTTA TGGAATCCTG 
GGGGTTCAGA TGTTTGGCAC ATTCAATCAT CATTGCGTCA CCAATGAAAC AGAAAAAGAG 
AAAGTGACAT GGAACAGCCT AGCAATCCCG GATACACACT GCTCTCCGAA CGGGGAGGGA 
TATCAGTGTC CAGTGGGCTT TAAGTGTGTG GATCTGGAAG ATTATGGACT CAGTAGACAA 
GAACTTGGAT ACAGTGGATT CAATGAGCTT GGCACCAGTA TATTCACTGT ATATGAGGCT 
GCGTCTCAGG AGGGCTGGGT CTTCATCATG TATCGAGCCA TCGACAGCTT CCCACGCTGG 
CGCTCATATT TCTACTTCCT CACGCTCATC TTCTTCCTCG CCTGGCTGGT CAAGAATGTG 
TTCATTGCTG TGATTATCGA AACGTTTGCT GAGATCAGAG TGCAGTTCCA GCAGATGTGG 
GGATCCCGGA GCAGCACCAC GTCAACAGCT ACTACACAGA TGTTTCACGA GGACGCGTCT 
GGAGGATGGC AGCTCGTGGC GGTGGACGTG AATAAACCAC ATGGCCGGGC GCCTGCTTGC 
CTCCAGCAAC TGATGCGATC TTCAGTTTTC CACATGTTCA TCCTCAGTAT GGTGGCTGTT 
GATGTGATTG TAGCTGCGAG CAATTACCAC AGAGGTGACC ATAAACTCAC CAATGATGAG 
TTCTACCTGG CTGAGGTCGC CTTCACTGTG CTGTTTGATT TGGAGGCTCT GCTGAAAATC 
TGGTGTTTGG GCTTCACTGG ATACATCAGT TCATCGCTGC ACAAGTTTGA GTCGCTGCTT 
GTAGTGGGCA CCACCTTGCA CATCTACCCT GACCTTTACC ACTCGCAGTT CACCTACTTT 
CAGGTTTTGC GAGTGGTCCG TCTAATAAAA ATCTCCCCTG CACTAGAGGA CTTTGTGTAC 
AAGATCTTTG GTCCAGGGAA GAAACTGGGC AGTCTAGTGG TGTTTACTGC CAGTCTGCTC 
ATAGTGATGT CTGCCATCAG TCTGCAGATG TTCTGCTTTG TGGAGGATTT GGATCGTTTC 
ACCACATTTC CACGGGCGTT TATGTCCATG TTCCAGATTC TCACGCAGGA GGGCTGGGTG 
GATGTCATGG ACCAAACACT GGTGGCTGTG GGACAGATGT GGGCTCCGGT GGTGGCCATA 
TACTTTATTC TGTATCACCT TTTTGCCACA CTGATCCTTC TGAGTCTTTT TGTTGCTGTT 
ATTTTGGACA ATCTTGAGTT GGATGAAGAC CTGAAAAAGT TAAAACAGCT GAAGCAGAGT 
GAAGCCAATG CTGATACAAA AGAGAAACTT CCTTTGCGAC TGCGAATCTT TGAGAAGTTC 
CCCAATCGAC CGCAGATGGT GAAAATCTCC AAGCTGCCTT CAGACTTCAC TGTGCCCAGA 
ATCAGGGAGA GTTTCATGAA GCAGTTCATC GACCGACAGC AGCAGGACCC TAGCTGTCTG 
TTCCGTCGCC TTCCCTCAGC ATCATCCTCG TCTTGCGACC ATTCCAAACG CTCTGCCATT 
GAGGATAATA AATACATAGA TCAGAAGCTA CGAAAGTCAG TATTTAGCAT CCGGGCTCGC 
AACCTAATGG AAAAGGAAAT CACTGTCAAC AAAATTTTGC GAGCCTGCAC CAGGCAGCGC 
ATGCTGAGTG GCTCATTTGA GGGTCAGCCA ACTAAAGAGA GATCCATTCT CAGTGTCCAG 
CATCACATCC GCCAAGAACG CCGGTCCCTC AGACATGGCT CTACCAGCCA GAGAATCAGC 
AGAGGGAAAT CCTTGGAAAC ACTCACTCAG GACCACTCCA GCACGGTCCG CTACCGTAAT 
GCCCAGCGGG AGGACAGTGA AATCAAGATG ATACAAGAGA AGAAGGAGCA GGCTGAGATG 
AAACGAAAAG TCCAAGAGGA GGAACTTCGG GAAAACCATC CATACTTTGA CAAGCCACTC 
TTCATCGTTG GTAGAGAGCA TCGATTCAGA AACTTTTGCC GAATGATTGT TAGAGCCCGT 
TTTAATGCGT CAAATACGGA TCCCAATACA GGCGCAGTGA ACAGCACAAA ATACCATCAA 
TTATATGATT TGCTGGGCTT GGTAACCTAT CTGGACTGGG TGATGATCGT GGTCACTATC 
TGCTCTTGTA TTTCCATGAT GTTTGAGTCT CCTTTTACTA GAGTCATGCA TGTCCCAACC 
CTGCAGATTG GAGAATATGT GTTCGTCATC TTTATGAGCA TTGAGCTGAA CCTGAAGATT 
ATGGCAGATG GCCTGTTCTT CACTCCCACT GCAGTCATCC GAGACTTTGG CGGCGTCATG 
GACATCTTCA TCTACTTAGT AAGTTTGATC TTTTTATGCT GGCTGCCAAA TAATGTGCCC 
CCTGAGTCTG GTGCCCAGCT CCTCATGATG TTACGTTGCT TCCGGCCACT GAGAATCTTT 
AAACTGGTGC CCCAAATGAG GAAGGTGGTT CGAGAGGTCC TCAAGGGTTT CAAAGAGATT 
TTTCTGGTCT CTATTCTTCT CCTGACACTA ATGCTGGTGT TCGCAACCTT CGGGGTGCAA 
CTCTTTGCTG GAAAACTTGC CAAATGTAAT GATCCTCATA TTTCTAGCAA GGATGACTGT 
CACGGCATCT TTAGAATCAA TGTGAGCATT TCAAAGAACC TGAATTTGAA GCTCAAGGAT 
GGGGAGAAGA AACCAGGATT TTGGGTGCCA AGAGTTTGGG CAAACCCTCG AAACTTCAAT 
TTTGATAATG TGGGCAATGC CATGCTGGCT CTGTTTGAAG TGTTGTCGCT GAAGGGTTGG 
GTGGAGGTGA GAGATGTCAT CATTCACAGA GTAGGACCGA TTCATGGTAT CTACATCCAT 
GTCTTTGTGT TCTTGGGATG TATGATCGGA CTCACCCTTT TTGTTGGCGT TGTCATTGCC 
AACTTTAATG AAAATAAGGG CACTGCTCTG TTGACTGTGG ATCAGAGGAG ATGGGAAGAT 
TTAAAGAGTC GACTCAAGAT TGCTCAGCCC CTCCACCTCC CTCCACGTCC AGAAAATGGT 
GGATTTCGTG CAAAGATGTA TGACATCACC CAACATCCCT TTTTCAAGCG AGGGATTGCT 
GTGCTGGTTT TAGCACAGTC TGTCCTCCTG TCGGTTAAGT GGGATGAGCT TGGCGCAGTT 
ACGTTTCCCT TGGCCACAAT GTCAGTGGTT TTCACTTTCA TTTTTGTTTT GGAGGTGACT 
ATGAAGCTGA TAGCCATGTC ACCAGCTGGA TACTGGCAGA GCAGGAGGAA CAGATATGAC 
CTACTCGTCA CATCCCTCGG GCTCATATGG ATCATTCTGC ACTTCGCTTT ACAGAATGCT 
TACACATACA TGATGGGCAC CTGTGTGATA GTATTCAGAT TTTTCACCAT ATGCGGAAAG 
CATGTGACGT TAAAGATGCT TCTGCTTACT GTGGTCGTCA GTATGTACAA GAGTTTCTTC 
ATTATCGTGG GCATGTTTTT GCTGCTGCTG TGCTACGCTT TCGCCGGCGT CGTTCTTTTT 
GGCACCGTCA AATACGGAGA GAACATTAAC AGACACGCAA ACTTCTCCAC CGCAGGGAAA 
GCCATCACCG TACTTATTCG AATAGTCACA GGTGAAGACT GGAATAAGAT CATGCATGAC 
TGCATGGTGC AGCCGCCGTT CTGCACCCCA GACAAACATC GCTACTGGGA GACGGACTGC 
GGCAACTACG CTGGAGCCCT CATTTACTTC TGCTCCTTCT ATGTCATCAT CGCTTACATC 
ATGCTCAACC TGCTCGTAGC CATCATTGTG GAGAATTTCT CTTTGTTCTA CTCCACCGAA 
GAGGACCAGT TGCTAAGCTA CAACGACCTG CGTCACTTCC AGATCATCTG GAACATGGTG 
GATGACAAGC GGGAGGGAGT GATTCCGACC TCTCGGGTGA AGTTTCTGCT CCGTCTGCTG 
AGAGGTCGTC TGGAAGTGGA TCTGGACAAA GACAAGCTTC TCTTTAAACA CATGTGTTAT 
GAGATGGAGC GTCTGCACAA TGGAGGAGAC GTCACCTTTC ATGATGTACT GAGCATGCTG 
TCGTATCGTT CAGTTGATAT CCGCAAGAGT CTTCAGCTGG AGGAGCTGTT GGCAAGAGAG 
CAGCTTGAAT ACACTATAGA GGAGGAAGTG GCCAAACAAA CCATCCGCAT GTGGTTGAAG 
AAGTGCCTGA AACGCATCAG AGCCAAACAG CAGCAGTCAT GCAGCATCAT CCTCAGTCTG 
AGAGAGAGTC AACAGCAAGA CCTGCGGCGG CTGCTCAACC CCCCCAGCAT CGAGACCACC 
GTCCCCAGCG AAGACACCAA CACACACAAC CAGGACAATC CCACCCAGCC TGAGAACAGC 
GGCCTGCAGA CGTTACTGAG CCCCACTCTG TCAGACCGCA GTGGATACAG ACAGGACTCT 
GCTGACCGAC CCCAGAGGAA GCTGGGACAG TGGAGACTTC CTGCAGGCCG CACAAGCGTG 
AAGTCCATGG TGTGTAAGAT GAACCCGGTG ACTGACGAGG CGTCCTCAGG CTCTGAAGTT 
AAGAAGTGGT GGACGCGTCA ACTGACTGTG GAAAGTGATG AAAGTGGAGA CGACCTCATT 
GATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_005167698.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167755.1 Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa15242
Clone ID Related Accession (Same CDS sequence)	XM_005167698.3 , XM_017357669.1 , XM_005167698.4
Accession Version	XM_005167698.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sodium leak channel non-selective protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAGTG CTGGAGCAAT TTCAGCTTTA CTTTTCCCGT TGATCCACTC TCTCTATAGC 
ACAGCGAGCC CAAGCATGCT GAAGAGGAAG CAGAGCTCGC GGGTCGAGGC CCAGCCTATG 
ACTGACTTTG GGCCAGACGA AACACTGACA GACAGTGCTG ACATTCTTTG GATCAATAAA 
CCATGGGTTC ACTCTCTCCT GCGGGCCTGT GCCATAATCA GTGTTATTTC GGTTTGTATG 
AATACACCTA AGACCTTTGA GCACTACCCT CCCCTACAGT ATGTCACCTT CGCCCTGGAC 
ACACTGCTGA TGTTCCTGTA CACCGCAGAG ATGATCGCCA AAATGCATAT CAGGGGCATC 
ATCAAGGGAG AAAACTCCTA TGTTAAAGAC CGCTGGTGCA TGTTTGATGG CTTCATGGTG 
GTCTTCCTCT GGGTCTCTCT TGTGCTTCAG GTGTTTGAAA TAGCGGAGCT CGTGGACCAA 
ATGTCACCCT GGGGAATGTT ACGCATACCG AGGGCCCTTA TTATGATCCG AGTCTTCCGA 
ATCTATTTCC GATTTGAATT GCCACGTAGC CGGATAACCA ACATTCTCAA GCGCTCAGGG 
GAGCAGATCT GGAGTGTGAC CATATTTTTG CTATTCTTCC TTCTTCTTTA TGGAATCCTG 
GGGGTTCAGA TGTTTGGCAC ATTCAATCAT CATTGCGTCA CCAATGAAAC AGAAAAAGAG 
AAAGTGACAT GGAACAGCCT AGCAATCCCG GATACACACT GCTCTCCGAA CGGGGAGGGA 
TATCAGTGTC CAGTGGGCTT TAAGTGTGTG GATCTGGAAG ATTATGGACT CAGTAGACAA 
GAACTTGGAT ACAGTGGATT CAATGAGCTT GGCACCAGTA TATTCACTGT ATATGAGGCT 
GCGTCTCAGG AGGGCTGGGT CTTCATCATG TATCGAGCCA TCGACAGCTT CCCACGCTGG 
CGCTCATATT TCTACTTCCT CACGCTCATC TTCTTCCTCG CCTGGCTGGT CAAGAATGTG 
TTCATTGCTG TGATTATCGA AACGTTTGCT GAGATCAGAG TGCAGTTCCA GCAGATGTGG 
GGATCCCGGA GCAGCACCAC GTCAACAGCT ACTACACAGA TGTTTCACGA GGACGCGTCT 
GGAGGATGGC AGCTCGTGGC GGTGGACGTG AATAAACCAC ATGGCCGGGC GCCTGCTTGC 
CTCCAGCAAC TGATGCGATC TTCAGTTTTC CACATGTTCA TCCTCAGTAT GGTGGCTGTT 
GATGTGATTG TAGCTGCGAG CAATTACCAC AGAGGTGACC ATAAACTCAC CAATGATGAG 
TTCTACCTGG CTGAGGTCGC CTTCACTGTG CTGTTTGATT TGGAGGCTCT GCTGAAAATC 
TGGTGTTTGG GCTTCACTGG ATACATCAGT TCATCGCTGC ACAAGTTTGA GTCGCTGCTT 
GTAGTGGGCA CCACCTTGCA CATCTACCCT GACCTTTACC ACTCGCAGTT CACCTACTTT 
CAGGTTTTGC GAGTGGTCCG TCTAATAAAA ATCTCCCCTG CACTAGAGGA CTTTGTGTAC 
AAGATCTTTG GTCCAGGGAA GAAACTGGGC AGTCTAGTGG TGTTTACTGC CAGTCTGCTC 
ATAGTGATGT CTGCCATCAG TCTGCAGATG TTCTGCTTTG TGGAGGATTT GGATCGTTTC 
ACCACATTTC CACGGGCGTT TATGTCCATG TTCCAGATTC TCACGCAGGA GGGCTGGGTG 
GATGTCATGG ACCAAACACT GGTGGCTGTG GGACAGATGT GGGCTCCGGT GGTGGCCATA 
TACTTTATTC TGTATCACCT TTTTGCCACA CTGATCCTTC TGAGTCTTTT TGTTGCTGTT 
ATTTTGGACA ATCTTGAGTT GGATGAAGAC CTGAAAAAGT TAAAACAGCT GAAGCAGAGT 
GAAGCCAATG CTGATACAAA AGAGAAACTT CCTTTGCGAC TGCGAATCTT TGAGAAGTTC 
CCCAATCGAC CGCAGATGGT GAAAATCTCC AAGCTGCCTT CAGACTTCAC TGTGCCCAGA 
ATCAGGGAGA GTTTCATGAA GCAGTTCATC GACCGACAGC AGCAGGACCC TAGCTGTCTG 
TTCCGTCGCC TTCCCTCAGC ATCATCCTCG TCTTGCGACC ATTCCAAACG CTCTGCCATT 
GAGGATAATA AATACATAGA TCAGAAGCTA CGAAAGTCAG TATTTAGCAT CCGGGCTCGC 
AACCTAATGG AAAAGGAAAT CACTGTCAAC AAAATTTTGC GAGCCTGCAC CAGGCAGCGC 
ATGCTGAGTG GCTCATTTGA GGGTCAGCCA ACTAAAGAGA GATCCATTCT CAGTGTCCAG 
CATCACATCC GCCAAGAACG CCGGTCCCTC AGACATGGCT CTACCAGCCA GAGAATCAGC 
AGAGGGAAAT CCTTGGAAAC ACTCACTCAG GACCACTCCA GCACGGTCCG CTACCGTAAT 
GCCCAGCGGG AGGACAGTGA AATCAAGATG ATACAAGAGA AGAAGGAGCA GGCTGAGATG 
AAACGAAAAG TCCAAGAGGA GGAACTTCGG GAAAACCATC CATACTTTGA CAAGCCACTC 
TTCATCGTTG GTAGAGAGCA TCGATTCAGA AACTTTTGCC GAATGATTGT TAGAGCCCGT 
TTTAATGCGT CAAATACGGA TCCCAATACA GGCGCAGTGA ACAGCACAAA ATACCATCAA 
TTATATGATT TGCTGGGCTT GGTAACCTAT CTGGACTGGG TGATGATCGT GGTCACTATC 
TGCTCTTGTA TTTCCATGAT GTTTGAGTCT CCTTTTACTA GAGTCATGCA TGTCCCAACC 
CTGCAGATTG GAGAATATGT GTTCGTCATC TTTATGAGCA TTGAGCTGAA CCTGAAGATT 
ATGGCAGATG GCCTGTTCTT CACTCCCACT GCAGTCATCC GAGACTTTGG CGGCGTCATG 
GACATCTTCA TCTACTTAGT AAGTTTGATC TTTTTATGCT GGCTGCCAAA TAATGTGCCC 
CCTGAGTCTG GTGCCCAGCT CCTCATGATG TTACGTTGCT TCCGGCCACT GAGAATCTTT 
AAACTGGTGC CCCAAATGAG GAAGGTGGTT CGAGAGGTCC TCAAGGGTTT CAAAGAGATT 
TTTCTGGTCT CTATTCTTCT CCTGACACTA ATGCTGGTGT TCGCAACCTT CGGGGTGCAA 
CTCTTTGCTG GAAAACTTGC CAAATGTAAT GATCCTCATA TTTCTAGCAA GGATGACTGT 
CACGGCATCT TTAGAATCAA TGTGAGCATT TCAAAGAACC TGAATTTGAA GCTCAAGGAT 
GGGGAGAAGA AACCAGGATT TTGGGTGCCA AGAGTTTGGG CAAACCCTCG AAACTTCAAT 
TTTGATAATG TGGGCAATGC CATGCTGGCT CTGTTTGAAG TGTTGTCGCT GAAGGGTTGG 
GTGGAGGTGA GAGATGTCAT CATTCACAGA GTAGGACCGA TTCATGGTAT CTACATCCAT 
GTCTTTGTGT TCTTGGGATG TATGATCGGA CTCACCCTTT TTGTTGGCGT TGTCATTGCC 
AACTTTAATG AAAATAAGGG CACTGCTCTG TTGACTGTGG ATCAGAGGAG ATGGGAAGAT 
TTAAAGAGTC GACTCAAGAT TGCTCAGCCC CTCCACCTCC CTCCACGTCC AGAAAATGGT 
GGATTTCGTG CAAAGATGTA TGACATCACC CAACATCCCT TTTTCAAGCG AGGGATTGCT 
GTGCTGGTTT TAGCACAGTC TGTCCTCCTG TCGGTTAAGT GGGATGAGCT TGGCGCAGTT 
ACGTTTCCCT TGGCCACAAT GTCAGTGGTT TTCACTTTCA TTTTTGTTTT GGAGGTGACT 
ATGAAGCTGA TAGCCATGTC ACCAGCTGGA TACTGGCAGA GCAGGAGGAA CAGATATGAC 
CTACTCGTCA CATCCCTCGG GCTCATATGG ATCATTCTGC ACTTCGCTTT ACAGAATGCT 
TACACATACA TGATGGGCAC CTGTGTGATA GTATTCAGAT TTTTCACCAT ATGCGGAAAG 
CATGTGACGT TAAAGATGCT TCTGCTTACT GTGGTCGTCA GTATGTACAA GAGTTTCTTC 
ATTATCGTGG GCATGTTTTT GCTGCTGCTG TGCTACGCTT TCGCCGGCGT CGTTCTTTTT 
GGCACCGTCA AATACGGAGA GAACATTAAC AGACACGCAA ACTTCTCCAC CGCAGGGAAA 
GCCATCACCG TACTTATTCG AATAGTCACA GGTGAAGACT GGAATAAGAT CATGCATGAC 
TGCATGGTGC AGCCGCCGTT CTGCACCCCA GACAAACATC GCTACTGGGA GACGGACTGC 
GGCAACTACG CTGGAGCCCT CATTTACTTC TGCTCCTTCT ATGTCATCAT CGCTTACATC 
ATGCTCAACC TGCTCGTAGC CATCATTGTG GAGAATTTCT CTTTGTTCTA CTCCACCGAA 
GAGGACCAGT TGCTAAGCTA CAACGACCTG CGTCACTTCC AGATCATCTG GAACATGGTG 
GATGACAAGC GGGAGGGAGT GATTCCGACC TCTCGGGTGA AGTTTCTGCT CCGTCTGCTG 
AGAGGTCGTC TGGAAGTGGA TCTGGACAAA GACAAGCTTC TCTTTAAACA CATGTGTTAT 
GAGATGGAGC GTCTGCACAA TGGAGGAGAC GTCACCTTTC ATGATGTACT GAGCATGCTG 
TCGTATCGTT CAGTTGATAT CCGCAAGAGT CTTCAGCTGG AGGAGCTGTT GGCAAGAGAG 
CAGCTTGAAT ACACTATAGA GGAGGAAGTG GCCAAACAAA CCATCCGCAT GTGGTTGAAG 
AAGTGCCTGA AACGCATCAG AGCCAAACAG CAGCAGTCAT GCAGCATCAT CCTCAGTCTG 
AGAGAGAGTC AACAGCAAGA CCTGCGGCGG CTGCTCAACC CCCCCAGCAT CGAGACCACC 
GTCCCCAGCG AAGACACCAA CACACACAAC CAGGACAATC CCACCCAGCC TGAGCAGAAC 
AGCGGCCTGC AGACGTTACT GAGCCCCACT CTGTCAGACC GCAGTGGATA CAGACAGGAC 
TCTGCTGACC GACCCCAGAG GAAGCTGGGA CAGTGGAGAC TTCCTGCAGG CCGCACAAGC 
GTGAAGTCCA TGGTGTGTAA GATGAACCCG GTGACTGACG AGGCGTCCTC AGGCTCTGAA 
GTTAAGAAGT GGTGGACGCG TCAACTGACT GTGGAAAGTG ATGAAAGTGG AGACGACCTC 
ATTGATATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167755.1
CDS	235..5466
Translation	MCSAGAISALLFPLIHSLYSTASPSMLKRKQSSRVEAQPMTDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIKGENSYVKDRWCMFDGFMVVFLWVSLVLQVFEIAELVDQMSPWGMLRIPRALIMIRVFRIYFRFELPRSRITNILKRSGEQIWSVTIFLLFFLLLYGILGVQMFGTFNHHCVTNETEKEKVTWNSLAIPDTHCSPNGEGYQCPVGFKCVDLEDYGLSRQELGYSGFNELGTSIFTVYEAASQEGWVFIMYRAIDSFPRWRSYFYFLTLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDASGGWQLVAVDVNKPHGRAPACLQQLMRSSVFHMFILSMVAVDVIVAASNYHRGDHKLTNDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFESLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEDLDRFTTFPRAFMSMFQILTQEGWVDVMDQTLVAVGQMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPRIRESFMKQFIDRQQQDPSCLFRRLPSASSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLMEKEITVNKILRACTRQRMLSGSFEGQPTKERSILSVQHHIRQERRSLRHGSTSQRISRGKSLETLTQDHSSTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRMIVRARFNASNTDPNTGAVNSTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFTRVMHVPTLQIGEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWLPNNVPPESGAQLLMMLRCFRPLRIFKLVPQMRKVVREVLKGFKEIFLVSILLLTLMLVFATFGVQLFAGKLAKCNDPHISSKDDCHGIFRINVSISKNLNLKLKDGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPENGGFRAKMYDITQHPFFKRGIAVLVLAQSVLLSVKWDELGAVTFPLATMSVVFTFIFVLEVTMKLIAMSPAGYWQSRRNRYDLLVTSLGLIWIILHFALQNAYTYMMGTCVIVFRFFTICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLIRIVTGEDWNKIMHDCMVQPPFCTPDKHRYWETDCGNYAGALIYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTSRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIILSLRESQQQDLRRLLNPPSIETTVPSEDTNTHNQDNPTQPEQNSGLQTLLSPTLSDRSGYRQDSADRPQRKLGQWRLPAGRTSVKSMVCKMNPVTDEASSGSEVKKWWTRQLTVESDESGDDLIDI

Target ORF information:

RefSeq Version	XM_005167698.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167698.3

ORF Insert Sequence:

ATGTGCAGTG CTGGAGCAAT TTCAGCTTTA CTTTTCCCGT TGATCCACTC TCTCTATAGC 
ACAGCGAGCC CAAGCATGCT GAAGAGGAAG CAGAGCTCGC GGGTCGAGGC CCAGCCTATG 
ACTGACTTTG GGCCAGACGA AACACTGACA GACAGTGCTG ACATTCTTTG GATCAATAAA 
CCATGGGTTC ACTCTCTCCT GCGGGCCTGT GCCATAATCA GTGTTATTTC GGTTTGTATG 
AATACACCTA AGACCTTTGA GCACTACCCT CCCCTACAGT ATGTCACCTT CGCCCTGGAC 
ACACTGCTGA TGTTCCTGTA CACCGCAGAG ATGATCGCCA AAATGCATAT CAGGGGCATC 
ATCAAGGGAG AAAACTCCTA TGTTAAAGAC CGCTGGTGCA TGTTTGATGG CTTCATGGTG 
GTCTTCCTCT GGGTCTCTCT TGTGCTTCAG GTGTTTGAAA TAGCGGAGCT CGTGGACCAA 
ATGTCACCCT GGGGAATGTT ACGCATACCG AGGGCCCTTA TTATGATCCG AGTCTTCCGA 
ATCTATTTCC GATTTGAATT GCCACGTAGC CGGATAACCA ACATTCTCAA GCGCTCAGGG 
GAGCAGATCT GGAGTGTGAC CATATTTTTG CTATTCTTCC TTCTTCTTTA TGGAATCCTG 
GGGGTTCAGA TGTTTGGCAC ATTCAATCAT CATTGCGTCA CCAATGAAAC AGAAAAAGAG 
AAAGTGACAT GGAACAGCCT AGCAATCCCG GATACACACT GCTCTCCGAA CGGGGAGGGA 
TATCAGTGTC CAGTGGGCTT TAAGTGTGTG GATCTGGAAG ATTATGGACT CAGTAGACAA 
GAACTTGGAT ACAGTGGATT CAATGAGCTT GGCACCAGTA TATTCACTGT ATATGAGGCT 
GCGTCTCAGG AGGGCTGGGT CTTCATCATG TATCGAGCCA TCGACAGCTT CCCACGCTGG 
CGCTCATATT TCTACTTCCT CACGCTCATC TTCTTCCTCG CCTGGCTGGT CAAGAATGTG 
TTCATTGCTG TGATTATCGA AACGTTTGCT GAGATCAGAG TGCAGTTCCA GCAGATGTGG 
GGATCCCGGA GCAGCACCAC GTCAACAGCT ACTACACAGA TGTTTCACGA GGACGCGTCT 
GGAGGATGGC AGCTCGTGGC GGTGGACGTG AATAAACCAC ATGGCCGGGC GCCTGCTTGC 
CTCCAGCAAC TGATGCGATC TTCAGTTTTC CACATGTTCA TCCTCAGTAT GGTGGCTGTT 
GATGTGATTG TAGCTGCGAG CAATTACCAC AGAGGTGACC ATAAACTCAC CAATGATGAG 
TTCTACCTGG CTGAGGTCGC CTTCACTGTG CTGTTTGATT TGGAGGCTCT GCTGAAAATC 
TGGTGTTTGG GCTTCACTGG ATACATCAGT TCATCGCTGC ACAAGTTTGA GTCGCTGCTT 
GTAGTGGGCA CCACCTTGCA CATCTACCCT GACCTTTACC ACTCGCAGTT CACCTACTTT 
CAGGTTTTGC GAGTGGTCCG TCTAATAAAA ATCTCCCCTG CACTAGAGGA CTTTGTGTAC 
AAGATCTTTG GTCCAGGGAA GAAACTGGGC AGTCTAGTGG TGTTTACTGC CAGTCTGCTC 
ATAGTGATGT CTGCCATCAG TCTGCAGATG TTCTGCTTTG TGGAGGATTT GGATCGTTTC 
ACCACATTTC CACGGGCGTT TATGTCCATG TTCCAGATTC TCACGCAGGA GGGCTGGGTG 
GATGTCATGG ACCAAACACT GGTGGCTGTG GGACAGATGT GGGCTCCGGT GGTGGCCATA 
TACTTTATTC TGTATCACCT TTTTGCCACA CTGATCCTTC TGAGTCTTTT TGTTGCTGTT 
ATTTTGGACA ATCTTGAGTT GGATGAAGAC CTGAAAAAGT TAAAACAGCT GAAGCAGAGT 
GAAGCCAATG CTGATACAAA AGAGAAACTT CCTTTGCGAC TGCGAATCTT TGAGAAGTTC 
CCCAATCGAC CGCAGATGGT GAAAATCTCC AAGCTGCCTT CAGACTTCAC TGTGCCCAGA 
ATCAGGGAGA GTTTCATGAA GCAGTTCATC GACCGACAGC AGCAGGACCC TAGCTGTCTG 
TTCCGTCGCC TTCCCTCAGC ATCATCCTCG TCTTGCGACC ATTCCAAACG CTCTGCCATT 
GAGGATAATA AATACATAGA TCAGAAGCTA CGAAAGTCAG TATTTAGCAT CCGGGCTCGC 
AACCTAATGG AAAAGGAAAT CACTGTCAAC AAAATTTTGC GAGCCTGCAC CAGGCAGCGC 
ATGCTGAGTG GCTCATTTGA GGGTCAGCCA ACTAAAGAGA GATCCATTCT CAGTGTCCAG 
CATCACATCC GCCAAGAACG CCGGTCCCTC AGACATGGCT CTACCAGCCA GAGAATCAGC 
AGAGGGAAAT CCTTGGAAAC ACTCACTCAG GACCACTCCA GCACGGTCCG CTACCGTAAT 
GCCCAGCGGG AGGACAGTGA AATCAAGATG ATACAAGAGA AGAAGGAGCA GGCTGAGATG 
AAACGAAAAG TCCAAGAGGA GGAACTTCGG GAAAACCATC CATACTTTGA CAAGCCACTC 
TTCATCGTTG GTAGAGAGCA TCGATTCAGA AACTTTTGCC GAATGATTGT TAGAGCCCGT 
TTTAATGCGT CAAATACGGA TCCCAATACA GGCGCAGTGA ACAGCACAAA ATACCATCAA 
TTATATGATT TGCTGGGCTT GGTAACCTAT CTGGACTGGG TGATGATCGT GGTCACTATC 
TGCTCTTGTA TTTCCATGAT GTTTGAGTCT CCTTTTACTA GAGTCATGCA TGTCCCAACC 
CTGCAGATTG GAGAATATGT GTTCGTCATC TTTATGAGCA TTGAGCTGAA CCTGAAGATT 
ATGGCAGATG GCCTGTTCTT CACTCCCACT GCAGTCATCC GAGACTTTGG CGGCGTCATG 
GACATCTTCA TCTACTTAGT AAGTTTGATC TTTTTATGCT GGCTGCCAAA TAATGTGCCC 
CCTGAGTCTG GTGCCCAGCT CCTCATGATG TTACGTTGCT TCCGGCCACT GAGAATCTTT 
AAACTGGTGC CCCAAATGAG GAAGGTGGTT CGAGAGGTCC TCAAGGGTTT CAAAGAGATT 
TTTCTGGTCT CTATTCTTCT CCTGACACTA ATGCTGGTGT TCGCAACCTT CGGGGTGCAA 
CTCTTTGCTG GAAAACTTGC CAAATGTAAT GATCCTCATA TTTCTAGCAA GGATGACTGT 
CACGGCATCT TTAGAATCAA TGTGAGCATT TCAAAGAACC TGAATTTGAA GCTCAAGGAT 
GGGGAGAAGA AACCAGGATT TTGGGTGCCA AGAGTTTGGG CAAACCCTCG AAACTTCAAT 
TTTGATAATG TGGGCAATGC CATGCTGGCT CTGTTTGAAG TGTTGTCGCT GAAGGGTTGG 
GTGGAGGTGA GAGATGTCAT CATTCACAGA GTAGGACCGA TTCATGGTAT CTACATCCAT 
GTCTTTGTGT TCTTGGGATG TATGATCGGA CTCACCCTTT TTGTTGGCGT TGTCATTGCC 
AACTTTAATG AAAATAAGGG CACTGCTCTG TTGACTGTGG ATCAGAGGAG ATGGGAAGAT 
TTAAAGAGTC GACTCAAGAT TGCTCAGCCC CTCCACCTCC CTCCACGTCC AGAAAATGGT 
GGATTTCGTG CAAAGATGTA TGACATCACC CAACATCCCT TTTTCAAGCG AGGGATTGCT 
GTGCTGGTTT TAGCACAGTC TGTCCTCCTG TCGGTTAAGT GGGATGAGCT TGGCGCAGTT 
ACGTTTCCCT TGGCCACAAT GTCAGTGGTT TTCACTTTCA TTTTTGTTTT GGAGGTGACT 
ATGAAGCTGA TAGCCATGTC ACCAGCTGGA TACTGGCAGA GCAGGAGGAA CAGATATGAC 
CTACTCGTCA CATCCCTCGG GCTCATATGG ATCATTCTGC ACTTCGCTTT ACAGAATGCT 
TACACATACA TGATGGGCAC CTGTGTGATA GTATTCAGAT TTTTCACCAT ATGCGGAAAG 
CATGTGACGT TAAAGATGCT TCTGCTTACT GTGGTCGTCA GTATGTACAA GAGTTTCTTC 
ATTATCGTGG GCATGTTTTT GCTGCTGCTG TGCTACGCTT TCGCCGGCGT CGTTCTTTTT 
GGCACCGTCA AATACGGAGA GAACATTAAC AGACACGCAA ACTTCTCCAC CGCAGGGAAA 
GCCATCACCG TACTTATTCG AATAGTCACA GGTGAAGACT GGAATAAGAT CATGCATGAC 
TGCATGGTGC AGCCGCCGTT CTGCACCCCA GACAAACATC GCTACTGGGA GACGGACTGC 
GGCAACTACG CTGGAGCCCT CATTTACTTC TGCTCCTTCT ATGTCATCAT CGCTTACATC 
ATGCTCAACC TGCTCGTAGC CATCATTGTG GAGAATTTCT CTTTGTTCTA CTCCACCGAA 
GAGGACCAGT TGCTAAGCTA CAACGACCTG CGTCACTTCC AGATCATCTG GAACATGGTG 
GATGACAAGC GGGAGGGAGT GATTCCGACC TCTCGGGTGA AGTTTCTGCT CCGTCTGCTG 
AGAGGTCGTC TGGAAGTGGA TCTGGACAAA GACAAGCTTC TCTTTAAACA CATGTGTTAT 
GAGATGGAGC GTCTGCACAA TGGAGGAGAC GTCACCTTTC ATGATGTACT GAGCATGCTG 
TCGTATCGTT CAGTTGATAT CCGCAAGAGT CTTCAGCTGG AGGAGCTGTT GGCAAGAGAG 
CAGCTTGAAT ACACTATAGA GGAGGAAGTG GCCAAACAAA CCATCCGCAT GTGGTTGAAG 
AAGTGCCTGA AACGCATCAG AGCCAAACAG CAGCAGTCAT GCAGCATCAT CCTCAGTCTG 
AGAGAGAGTC AACAGCAAGA CCTGCGGCGG CTGCTCAACC CCCCCAGCAT CGAGACCACC 
GTCCCCAGCG AAGACACCAA CACACACAAC CAGGACAATC CCACCCAGCC TGAGCAGAAC 
AGCGGCCTGC AGACGTTACT GAGCCCCACT CTGTCAGACC GCAGTGGATA CAGACAGGAC 
TCTGCTGACC GACCCCAGAG GAAGCTGGGA CAGTGGAGAC TTCCTGCAGG CCGCACAAGC 
GTGAAGTCCA TGGTGTGTAA GATGAACCCG GTGACTGACG AGGCGTCCTC AGGCTCTGAA 
GTTAAGAAGT GGTGGACGCG TCAACTGACT GTGGAAAGTG ATGAAAGTGG AGACGACCTC 
ATTGATATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_017357669.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213158.1 Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15242
Clone ID Related Accession (Same CDS sequence)	XM_005167698.3 , XM_017357669.1 , XM_005167698.4
Accession Version	XM_017357669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAGTG CTGGAGCAAT TTCAGCTTTA CTTTTCCCGT TGATCCACTC TCTCTATAGC 
ACAGCGAGCC CAAGCATGCT GAAGAGGAAG CAGAGCTCGC GGGTCGAGGC CCAGCCTATG 
ACTGACTTTG GGCCAGACGA AACACTGACA GACAGTGCTG ACATTCTTTG GATCAATAAA 
CCATGGGTTC ACTCTCTCCT GCGGGCCTGT GCCATAATCA GTGTTATTTC GGTTTGTATG 
AATACACCTA AGACCTTTGA GCACTACCCT CCCCTACAGT ATGTCACCTT CGCCCTGGAC 
ACACTGCTGA TGTTCCTGTA CACCGCAGAG ATGATCGCCA AAATGCATAT CAGGGGCATC 
ATCAAGGGAG AAAACTCCTA TGTTAAAGAC CGCTGGTGCA TGTTTGATGG CTTCATGGTG 
GTCTTCCTCT GGGTCTCTCT TGTGCTTCAG GTGTTTGAAA TAGCGGAGCT CGTGGACCAA 
ATGTCACCCT GGGGAATGTT ACGCATACCG AGGGCCCTTA TTATGATCCG AGTCTTCCGA 
ATCTATTTCC GATTTGAATT GCCACGTAGC CGGATAACCA ACATTCTCAA GCGCTCAGGG 
GAGCAGATCT GGAGTGTGAC CATATTTTTG CTATTCTTCC TTCTTCTTTA TGGAATCCTG 
GGGGTTCAGA TGTTTGGCAC ATTCAATCAT CATTGCGTCA CCAATGAAAC AGAAAAAGAG 
AAAGTGACAT GGAACAGCCT AGCAATCCCG GATACACACT GCTCTCCGAA CGGGGAGGGA 
TATCAGTGTC CAGTGGGCTT TAAGTGTGTG GATCTGGAAG ATTATGGACT CAGTAGACAA 
GAACTTGGAT ACAGTGGATT CAATGAGCTT GGCACCAGTA TATTCACTGT ATATGAGGCT 
GCGTCTCAGG AGGGCTGGGT CTTCATCATG TATCGAGCCA TCGACAGCTT CCCACGCTGG 
CGCTCATATT TCTACTTCCT CACGCTCATC TTCTTCCTCG CCTGGCTGGT CAAGAATGTG 
TTCATTGCTG TGATTATCGA AACGTTTGCT GAGATCAGAG TGCAGTTCCA GCAGATGTGG 
GGATCCCGGA GCAGCACCAC GTCAACAGCT ACTACACAGA TGTTTCACGA GGACGCGTCT 
GGAGGATGGC AGCTCGTGGC GGTGGACGTG AATAAACCAC ATGGCCGGGC GCCTGCTTGC 
CTCCAGCAAC TGATGCGATC TTCAGTTTTC CACATGTTCA TCCTCAGTAT GGTGGCTGTT 
GATGTGATTG TAGCTGCGAG CAATTACCAC AGAGGTGACC ATAAACTCAC CAATGATGAG 
TTCTACCTGG CTGAGGTCGC CTTCACTGTG CTGTTTGATT TGGAGGCTCT GCTGAAAATC 
TGGTGTTTGG GCTTCACTGG ATACATCAGT TCATCGCTGC ACAAGTTTGA GTCGCTGCTT 
GTAGTGGGCA CCACCTTGCA CATCTACCCT GACCTTTACC ACTCGCAGTT CACCTACTTT 
CAGGTTTTGC GAGTGGTCCG TCTAATAAAA ATCTCCCCTG CACTAGAGGA CTTTGTGTAC 
AAGATCTTTG GTCCAGGGAA GAAACTGGGC AGTCTAGTGG TGTTTACTGC CAGTCTGCTC 
ATAGTGATGT CTGCCATCAG TCTGCAGATG TTCTGCTTTG TGGAGGATTT GGATCGTTTC 
ACCACATTTC CACGGGCGTT TATGTCCATG TTCCAGATTC TCACGCAGGA GGGCTGGGTG 
GATGTCATGG ACCAAACACT GGTGGCTGTG GGACAGATGT GGGCTCCGGT GGTGGCCATA 
TACTTTATTC TGTATCACCT TTTTGCCACA CTGATCCTTC TGAGTCTTTT TGTTGCTGTT 
ATTTTGGACA ATCTTGAGTT GGATGAAGAC CTGAAAAAGT TAAAACAGCT GAAGCAGAGT 
GAAGCCAATG CTGATACAAA AGAGAAACTT CCTTTGCGAC TGCGAATCTT TGAGAAGTTC 
CCCAATCGAC CGCAGATGGT GAAAATCTCC AAGCTGCCTT CAGACTTCAC TGTGCCCAGA 
ATCAGGGAGA GTTTCATGAA GCAGTTCATC GACCGACAGC AGCAGGACCC TAGCTGTCTG 
TTCCGTCGCC TTCCCTCAGC ATCATCCTCG TCTTGCGACC ATTCCAAACG CTCTGCCATT 
GAGGATAATA AATACATAGA TCAGAAGCTA CGAAAGTCAG TATTTAGCAT CCGGGCTCGC 
AACCTAATGG AAAAGGAAAT CACTGTCAAC AAAATTTTGC GAGCCTGCAC CAGGCAGCGC 
ATGCTGAGTG GCTCATTTGA GGGTCAGCCA ACTAAAGAGA GATCCATTCT CAGTGTCCAG 
CATCACATCC GCCAAGAACG CCGGTCCCTC AGACATGGCT CTACCAGCCA GAGAATCAGC 
AGAGGGAAAT CCTTGGAAAC ACTCACTCAG GACCACTCCA GCACGGTCCG CTACCGTAAT 
GCCCAGCGGG AGGACAGTGA AATCAAGATG ATACAAGAGA AGAAGGAGCA GGCTGAGATG 
AAACGAAAAG TCCAAGAGGA GGAACTTCGG GAAAACCATC CATACTTTGA CAAGCCACTC 
TTCATCGTTG GTAGAGAGCA TCGATTCAGA AACTTTTGCC GAATGATTGT TAGAGCCCGT 
TTTAATGCGT CAAATACGGA TCCCAATACA GGCGCAGTGA ACAGCACAAA ATACCATCAA 
TTATATGATT TGCTGGGCTT GGTAACCTAT CTGGACTGGG TGATGATCGT GGTCACTATC 
TGCTCTTGTA TTTCCATGAT GTTTGAGTCT CCTTTTACTA GAGTCATGCA TGTCCCAACC 
CTGCAGATTG GAGAATATGT GTTCGTCATC TTTATGAGCA TTGAGCTGAA CCTGAAGATT 
ATGGCAGATG GCCTGTTCTT CACTCCCACT GCAGTCATCC GAGACTTTGG CGGCGTCATG 
GACATCTTCA TCTACTTAGT AAGTTTGATC TTTTTATGCT GGCTGCCAAA TAATGTGCCC 
CCTGAGTCTG GTGCCCAGCT CCTCATGATG TTACGTTGCT TCCGGCCACT GAGAATCTTT 
AAACTGGTGC CCCAAATGAG GAAGGTGGTT CGAGAGGTCC TCAAGGGTTT CAAAGAGATT 
TTTCTGGTCT CTATTCTTCT CCTGACACTA ATGCTGGTGT TCGCAACCTT CGGGGTGCAA 
CTCTTTGCTG GAAAACTTGC CAAATGTAAT GATCCTCATA TTTCTAGCAA GGATGACTGT 
CACGGCATCT TTAGAATCAA TGTGAGCATT TCAAAGAACC TGAATTTGAA GCTCAAGGAT 
GGGGAGAAGA AACCAGGATT TTGGGTGCCA AGAGTTTGGG CAAACCCTCG AAACTTCAAT 
TTTGATAATG TGGGCAATGC CATGCTGGCT CTGTTTGAAG TGTTGTCGCT GAAGGGTTGG 
GTGGAGGTGA GAGATGTCAT CATTCACAGA GTAGGACCGA TTCATGGTAT CTACATCCAT 
GTCTTTGTGT TCTTGGGATG TATGATCGGA CTCACCCTTT TTGTTGGCGT TGTCATTGCC 
AACTTTAATG AAAATAAGGG CACTGCTCTG TTGACTGTGG ATCAGAGGAG ATGGGAAGAT 
TTAAAGAGTC GACTCAAGAT TGCTCAGCCC CTCCACCTCC CTCCACGTCC AGAAAATGGT 
GGATTTCGTG CAAAGATGTA TGACATCACC CAACATCCCT TTTTCAAGCG AGGGATTGCT 
GTGCTGGTTT TAGCACAGTC TGTCCTCCTG TCGGTTAAGT GGGATGAGCT TGGCGCAGTT 
ACGTTTCCCT TGGCCACAAT GTCAGTGGTT TTCACTTTCA TTTTTGTTTT GGAGGTGACT 
ATGAAGCTGA TAGCCATGTC ACCAGCTGGA TACTGGCAGA GCAGGAGGAA CAGATATGAC 
CTACTCGTCA CATCCCTCGG GCTCATATGG ATCATTCTGC ACTTCGCTTT ACAGAATGCT 
TACACATACA TGATGGGCAC CTGTGTGATA GTATTCAGAT TTTTCACCAT ATGCGGAAAG 
CATGTGACGT TAAAGATGCT TCTGCTTACT GTGGTCGTCA GTATGTACAA GAGTTTCTTC 
ATTATCGTGG GCATGTTTTT GCTGCTGCTG TGCTACGCTT TCGCCGGCGT CGTTCTTTTT 
GGCACCGTCA AATACGGAGA GAACATTAAC AGACACGCAA ACTTCTCCAC CGCAGGGAAA 
GCCATCACCG TACTTATTCG AATAGTCACA GGTGAAGACT GGAATAAGAT CATGCATGAC 
TGCATGGTGC AGCCGCCGTT CTGCACCCCA GACAAACATC GCTACTGGGA GACGGACTGC 
GGCAACTACG CTGGAGCCCT CATTTACTTC TGCTCCTTCT ATGTCATCAT CGCTTACATC 
ATGCTCAACC TGCTCGTAGC CATCATTGTG GAGAATTTCT CTTTGTTCTA CTCCACCGAA 
GAGGACCAGT TGCTAAGCTA CAACGACCTG CGTCACTTCC AGATCATCTG GAACATGGTG 
GATGACAAGC GGGAGGGAGT GATTCCGACC TCTCGGGTGA AGTTTCTGCT CCGTCTGCTG 
AGAGGTCGTC TGGAAGTGGA TCTGGACAAA GACAAGCTTC TCTTTAAACA CATGTGTTAT 
GAGATGGAGC GTCTGCACAA TGGAGGAGAC GTCACCTTTC ATGATGTACT GAGCATGCTG 
TCGTATCGTT CAGTTGATAT CCGCAAGAGT CTTCAGCTGG AGGAGCTGTT GGCAAGAGAG 
CAGCTTGAAT ACACTATAGA GGAGGAAGTG GCCAAACAAA CCATCCGCAT GTGGTTGAAG 
AAGTGCCTGA AACGCATCAG AGCCAAACAG CAGCAGTCAT GCAGCATCAT CCTCAGTCTG 
AGAGAGAGTC AACAGCAAGA CCTGCGGCGG CTGCTCAACC CCCCCAGCAT CGAGACCACC 
GTCCCCAGCG AAGACACCAA CACACACAAC CAGGACAATC CCACCCAGCC TGAGCAGAAC 
AGCGGCCTGC AGACGTTACT GAGCCCCACT CTGTCAGACC GCAGTGGATA CAGACAGGAC 
TCTGCTGACC GACCCCAGAG GAAGCTGGGA CAGTGGAGAC TTCCTGCAGG CCGCACAAGC 
GTGAAGTCCA TGGTGTGTAA GATGAACCCG GTGACTGACG AGGCGTCCTC AGGCTCTGAA 
GTTAAGAAGT GGTGGACGCG TCAACTGACT GTGGAAAGTG ATGAAAGTGG AGACGACCTC 
ATTGATATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213158.1
CDS	240..5471
Translation	MCSAGAISALLFPLIHSLYSTASPSMLKRKQSSRVEAQPMTDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIKGENSYVKDRWCMFDGFMVVFLWVSLVLQVFEIAELVDQMSPWGMLRIPRALIMIRVFRIYFRFELPRSRITNILKRSGEQIWSVTIFLLFFLLLYGILGVQMFGTFNHHCVTNETEKEKVTWNSLAIPDTHCSPNGEGYQCPVGFKCVDLEDYGLSRQELGYSGFNELGTSIFTVYEAASQEGWVFIMYRAIDSFPRWRSYFYFLTLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDASGGWQLVAVDVNKPHGRAPACLQQLMRSSVFHMFILSMVAVDVIVAASNYHRGDHKLTNDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFESLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEDLDRFTTFPRAFMSMFQILTQEGWVDVMDQTLVAVGQMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPRIRESFMKQFIDRQQQDPSCLFRRLPSASSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLMEKEITVNKILRACTRQRMLSGSFEGQPTKERSILSVQHHIRQERRSLRHGSTSQRISRGKSLETLTQDHSSTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRMIVRARFNASNTDPNTGAVNSTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFTRVMHVPTLQIGEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWLPNNVPPESGAQLLMMLRCFRPLRIFKLVPQMRKVVREVLKGFKEIFLVSILLLTLMLVFATFGVQLFAGKLAKCNDPHISSKDDCHGIFRINVSISKNLNLKLKDGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPENGGFRAKMYDITQHPFFKRGIAVLVLAQSVLLSVKWDELGAVTFPLATMSVVFTFIFVLEVTMKLIAMSPAGYWQSRRNRYDLLVTSLGLIWIILHFALQNAYTYMMGTCVIVFRFFTICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLIRIVTGEDWNKIMHDCMVQPPFCTPDKHRYWETDCGNYAGALIYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTSRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIILSLRESQQQDLRRLLNPPSIETTVPSEDTNTHNQDNPTQPEQNSGLQTLLSPTLSDRSGYRQDSADRPQRKLGQWRLPAGRTSVKSMVCKMNPVTDEASSGSEVKKWWTRQLTVESDESGDDLIDI

Target ORF information:

RefSeq Version	XM_017357669.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357669.1

ORF Insert Sequence:

ATGTGCAGTG CTGGAGCAAT TTCAGCTTTA CTTTTCCCGT TGATCCACTC TCTCTATAGC 
ACAGCGAGCC CAAGCATGCT GAAGAGGAAG CAGAGCTCGC GGGTCGAGGC CCAGCCTATG 
ACTGACTTTG GGCCAGACGA AACACTGACA GACAGTGCTG ACATTCTTTG GATCAATAAA 
CCATGGGTTC ACTCTCTCCT GCGGGCCTGT GCCATAATCA GTGTTATTTC GGTTTGTATG 
AATACACCTA AGACCTTTGA GCACTACCCT CCCCTACAGT ATGTCACCTT CGCCCTGGAC 
ACACTGCTGA TGTTCCTGTA CACCGCAGAG ATGATCGCCA AAATGCATAT CAGGGGCATC 
ATCAAGGGAG AAAACTCCTA TGTTAAAGAC CGCTGGTGCA TGTTTGATGG CTTCATGGTG 
GTCTTCCTCT GGGTCTCTCT TGTGCTTCAG GTGTTTGAAA TAGCGGAGCT CGTGGACCAA 
ATGTCACCCT GGGGAATGTT ACGCATACCG AGGGCCCTTA TTATGATCCG AGTCTTCCGA 
ATCTATTTCC GATTTGAATT GCCACGTAGC CGGATAACCA ACATTCTCAA GCGCTCAGGG 
GAGCAGATCT GGAGTGTGAC CATATTTTTG CTATTCTTCC TTCTTCTTTA TGGAATCCTG 
GGGGTTCAGA TGTTTGGCAC ATTCAATCAT CATTGCGTCA CCAATGAAAC AGAAAAAGAG 
AAAGTGACAT GGAACAGCCT AGCAATCCCG GATACACACT GCTCTCCGAA CGGGGAGGGA 
TATCAGTGTC CAGTGGGCTT TAAGTGTGTG GATCTGGAAG ATTATGGACT CAGTAGACAA 
GAACTTGGAT ACAGTGGATT CAATGAGCTT GGCACCAGTA TATTCACTGT ATATGAGGCT 
GCGTCTCAGG AGGGCTGGGT CTTCATCATG TATCGAGCCA TCGACAGCTT CCCACGCTGG 
CGCTCATATT TCTACTTCCT CACGCTCATC TTCTTCCTCG CCTGGCTGGT CAAGAATGTG 
TTCATTGCTG TGATTATCGA AACGTTTGCT GAGATCAGAG TGCAGTTCCA GCAGATGTGG 
GGATCCCGGA GCAGCACCAC GTCAACAGCT ACTACACAGA TGTTTCACGA GGACGCGTCT 
GGAGGATGGC AGCTCGTGGC GGTGGACGTG AATAAACCAC ATGGCCGGGC GCCTGCTTGC 
CTCCAGCAAC TGATGCGATC TTCAGTTTTC CACATGTTCA TCCTCAGTAT GGTGGCTGTT 
GATGTGATTG TAGCTGCGAG CAATTACCAC AGAGGTGACC ATAAACTCAC CAATGATGAG 
TTCTACCTGG CTGAGGTCGC CTTCACTGTG CTGTTTGATT TGGAGGCTCT GCTGAAAATC 
TGGTGTTTGG GCTTCACTGG ATACATCAGT TCATCGCTGC ACAAGTTTGA GTCGCTGCTT 
GTAGTGGGCA CCACCTTGCA CATCTACCCT GACCTTTACC ACTCGCAGTT CACCTACTTT 
CAGGTTTTGC GAGTGGTCCG TCTAATAAAA ATCTCCCCTG CACTAGAGGA CTTTGTGTAC 
AAGATCTTTG GTCCAGGGAA GAAACTGGGC AGTCTAGTGG TGTTTACTGC CAGTCTGCTC 
ATAGTGATGT CTGCCATCAG TCTGCAGATG TTCTGCTTTG TGGAGGATTT GGATCGTTTC 
ACCACATTTC CACGGGCGTT TATGTCCATG TTCCAGATTC TCACGCAGGA GGGCTGGGTG 
GATGTCATGG ACCAAACACT GGTGGCTGTG GGACAGATGT GGGCTCCGGT GGTGGCCATA 
TACTTTATTC TGTATCACCT TTTTGCCACA CTGATCCTTC TGAGTCTTTT TGTTGCTGTT 
ATTTTGGACA ATCTTGAGTT GGATGAAGAC CTGAAAAAGT TAAAACAGCT GAAGCAGAGT 
GAAGCCAATG CTGATACAAA AGAGAAACTT CCTTTGCGAC TGCGAATCTT TGAGAAGTTC 
CCCAATCGAC CGCAGATGGT GAAAATCTCC AAGCTGCCTT CAGACTTCAC TGTGCCCAGA 
ATCAGGGAGA GTTTCATGAA GCAGTTCATC GACCGACAGC AGCAGGACCC TAGCTGTCTG 
TTCCGTCGCC TTCCCTCAGC ATCATCCTCG TCTTGCGACC ATTCCAAACG CTCTGCCATT 
GAGGATAATA AATACATAGA TCAGAAGCTA CGAAAGTCAG TATTTAGCAT CCGGGCTCGC 
AACCTAATGG AAAAGGAAAT CACTGTCAAC AAAATTTTGC GAGCCTGCAC CAGGCAGCGC 
ATGCTGAGTG GCTCATTTGA GGGTCAGCCA ACTAAAGAGA GATCCATTCT CAGTGTCCAG 
CATCACATCC GCCAAGAACG CCGGTCCCTC AGACATGGCT CTACCAGCCA GAGAATCAGC 
AGAGGGAAAT CCTTGGAAAC ACTCACTCAG GACCACTCCA GCACGGTCCG CTACCGTAAT 
GCCCAGCGGG AGGACAGTGA AATCAAGATG ATACAAGAGA AGAAGGAGCA GGCTGAGATG 
AAACGAAAAG TCCAAGAGGA GGAACTTCGG GAAAACCATC CATACTTTGA CAAGCCACTC 
TTCATCGTTG GTAGAGAGCA TCGATTCAGA AACTTTTGCC GAATGATTGT TAGAGCCCGT 
TTTAATGCGT CAAATACGGA TCCCAATACA GGCGCAGTGA ACAGCACAAA ATACCATCAA 
TTATATGATT TGCTGGGCTT GGTAACCTAT CTGGACTGGG TGATGATCGT GGTCACTATC 
TGCTCTTGTA TTTCCATGAT GTTTGAGTCT CCTTTTACTA GAGTCATGCA TGTCCCAACC 
CTGCAGATTG GAGAATATGT GTTCGTCATC TTTATGAGCA TTGAGCTGAA CCTGAAGATT 
ATGGCAGATG GCCTGTTCTT CACTCCCACT GCAGTCATCC GAGACTTTGG CGGCGTCATG 
GACATCTTCA TCTACTTAGT AAGTTTGATC TTTTTATGCT GGCTGCCAAA TAATGTGCCC 
CCTGAGTCTG GTGCCCAGCT CCTCATGATG TTACGTTGCT TCCGGCCACT GAGAATCTTT 
AAACTGGTGC CCCAAATGAG GAAGGTGGTT CGAGAGGTCC TCAAGGGTTT CAAAGAGATT 
TTTCTGGTCT CTATTCTTCT CCTGACACTA ATGCTGGTGT TCGCAACCTT CGGGGTGCAA 
CTCTTTGCTG GAAAACTTGC CAAATGTAAT GATCCTCATA TTTCTAGCAA GGATGACTGT 
CACGGCATCT TTAGAATCAA TGTGAGCATT TCAAAGAACC TGAATTTGAA GCTCAAGGAT 
GGGGAGAAGA AACCAGGATT TTGGGTGCCA AGAGTTTGGG CAAACCCTCG AAACTTCAAT 
TTTGATAATG TGGGCAATGC CATGCTGGCT CTGTTTGAAG TGTTGTCGCT GAAGGGTTGG 
GTGGAGGTGA GAGATGTCAT CATTCACAGA GTAGGACCGA TTCATGGTAT CTACATCCAT 
GTCTTTGTGT TCTTGGGATG TATGATCGGA CTCACCCTTT TTGTTGGCGT TGTCATTGCC 
AACTTTAATG AAAATAAGGG CACTGCTCTG TTGACTGTGG ATCAGAGGAG ATGGGAAGAT 
TTAAAGAGTC GACTCAAGAT TGCTCAGCCC CTCCACCTCC CTCCACGTCC AGAAAATGGT 
GGATTTCGTG CAAAGATGTA TGACATCACC CAACATCCCT TTTTCAAGCG AGGGATTGCT 
GTGCTGGTTT TAGCACAGTC TGTCCTCCTG TCGGTTAAGT GGGATGAGCT TGGCGCAGTT 
ACGTTTCCCT TGGCCACAAT GTCAGTGGTT TTCACTTTCA TTTTTGTTTT GGAGGTGACT 
ATGAAGCTGA TAGCCATGTC ACCAGCTGGA TACTGGCAGA GCAGGAGGAA CAGATATGAC 
CTACTCGTCA CATCCCTCGG GCTCATATGG ATCATTCTGC ACTTCGCTTT ACAGAATGCT 
TACACATACA TGATGGGCAC CTGTGTGATA GTATTCAGAT TTTTCACCAT ATGCGGAAAG 
CATGTGACGT TAAAGATGCT TCTGCTTACT GTGGTCGTCA GTATGTACAA GAGTTTCTTC 
ATTATCGTGG GCATGTTTTT GCTGCTGCTG TGCTACGCTT TCGCCGGCGT CGTTCTTTTT 
GGCACCGTCA AATACGGAGA GAACATTAAC AGACACGCAA ACTTCTCCAC CGCAGGGAAA 
GCCATCACCG TACTTATTCG AATAGTCACA GGTGAAGACT GGAATAAGAT CATGCATGAC 
TGCATGGTGC AGCCGCCGTT CTGCACCCCA GACAAACATC GCTACTGGGA GACGGACTGC 
GGCAACTACG CTGGAGCCCT CATTTACTTC TGCTCCTTCT ATGTCATCAT CGCTTACATC 
ATGCTCAACC TGCTCGTAGC CATCATTGTG GAGAATTTCT CTTTGTTCTA CTCCACCGAA 
GAGGACCAGT TGCTAAGCTA CAACGACCTG CGTCACTTCC AGATCATCTG GAACATGGTG 
GATGACAAGC GGGAGGGAGT GATTCCGACC TCTCGGGTGA AGTTTCTGCT CCGTCTGCTG 
AGAGGTCGTC TGGAAGTGGA TCTGGACAAA GACAAGCTTC TCTTTAAACA CATGTGTTAT 
GAGATGGAGC GTCTGCACAA TGGAGGAGAC GTCACCTTTC ATGATGTACT GAGCATGCTG 
TCGTATCGTT CAGTTGATAT CCGCAAGAGT CTTCAGCTGG AGGAGCTGTT GGCAAGAGAG 
CAGCTTGAAT ACACTATAGA GGAGGAAGTG GCCAAACAAA CCATCCGCAT GTGGTTGAAG 
AAGTGCCTGA AACGCATCAG AGCCAAACAG CAGCAGTCAT GCAGCATCAT CCTCAGTCTG 
AGAGAGAGTC AACAGCAAGA CCTGCGGCGG CTGCTCAACC CCCCCAGCAT CGAGACCACC 
GTCCCCAGCG AAGACACCAA CACACACAAC CAGGACAATC CCACCCAGCC TGAGCAGAAC 
AGCGGCCTGC AGACGTTACT GAGCCCCACT CTGTCAGACC GCAGTGGATA CAGACAGGAC 
TCTGCTGACC GACCCCAGAG GAAGCTGGGA CAGTGGAGAC TTCCTGCAGG CCGCACAAGC 
GTGAAGTCCA TGGTGTGTAA GATGAACCCG GTGACTGACG AGGCGTCCTC AGGCTCTGAA 
GTTAAGAAGT GGTGGACGCG TCAACTGACT GTGGAAAGTG ATGAAAGTGG AGACGACCTC 
ATTGATATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_005167698.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167755.1 Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X2, mRNA.

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CloneID	ODa15242
Clone ID Related Accession (Same CDS sequence)	XM_005167698.3 , XM_017357669.1 , XM_005167698.4
Accession Version	XM_005167698.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167698.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAGTG CTGGAGCAAT TTCAGCTTTA CTTTTCCCGT TGATCCACTC TCTCTATAGC 
ACAGCGAGCC CAAGCATGCT GAAGAGGAAG CAGAGCTCGC GGGTCGAGGC CCAGCCTATG 
ACTGACTTTG GGCCAGACGA AACACTGACA GACAGTGCTG ACATTCTTTG GATCAATAAA 
CCATGGGTTC ACTCTCTCCT GCGGGCCTGT GCCATAATCA GTGTTATTTC GGTTTGTATG 
AATACACCTA AGACCTTTGA GCACTACCCT CCCCTACAGT ATGTCACCTT CGCCCTGGAC 
ACACTGCTGA TGTTCCTGTA CACCGCAGAG ATGATCGCCA AAATGCATAT CAGGGGCATC 
ATCAAGGGAG AAAACTCCTA TGTTAAAGAC CGCTGGTGCA TGTTTGATGG CTTCATGGTG 
GTCTTCCTCT GGGTCTCTCT TGTGCTTCAG GTGTTTGAAA TAGCGGAGCT CGTGGACCAA 
ATGTCACCCT GGGGAATGTT ACGCATACCG AGGGCCCTTA TTATGATCCG AGTCTTCCGA 
ATCTATTTCC GATTTGAATT GCCACGTAGC CGGATAACCA ACATTCTCAA GCGCTCAGGG 
GAGCAGATCT GGAGTGTGAC CATATTTTTG CTATTCTTCC TTCTTCTTTA TGGAATCCTG 
GGGGTTCAGA TGTTTGGCAC ATTCAATCAT CATTGCGTCA CCAATGAAAC AGAAAAAGAG 
AAAGTGACAT GGAACAGCCT AGCAATCCCG GATACACACT GCTCTCCGAA CGGGGAGGGA 
TATCAGTGTC CAGTGGGCTT TAAGTGTGTG GATCTGGAAG ATTATGGACT CAGTAGACAA 
GAACTTGGAT ACAGTGGATT CAATGAGCTT GGCACCAGTA TATTCACTGT ATATGAGGCT 
GCGTCTCAGG AGGGCTGGGT CTTCATCATG TATCGAGCCA TCGACAGCTT CCCACGCTGG 
CGCTCATATT TCTACTTCCT CACGCTCATC TTCTTCCTCG CCTGGCTGGT CAAGAATGTG 
TTCATTGCTG TGATTATCGA AACGTTTGCT GAGATCAGAG TGCAGTTCCA GCAGATGTGG 
GGATCCCGGA GCAGCACCAC GTCAACAGCT ACTACACAGA TGTTTCACGA GGACGCGTCT 
GGAGGATGGC AGCTCGTGGC GGTGGACGTG AATAAACCAC ATGGCCGGGC GCCTGCTTGC 
CTCCAGCAAC TGATGCGATC TTCAGTTTTC CACATGTTCA TCCTCAGTAT GGTGGCTGTT 
GATGTGATTG TAGCTGCGAG CAATTACCAC AGAGGTGACC ATAAACTCAC CAATGATGAG 
TTCTACCTGG CTGAGGTCGC CTTCACTGTG CTGTTTGATT TGGAGGCTCT GCTGAAAATC 
TGGTGTTTGG GCTTCACTGG ATACATCAGT TCATCGCTGC ACAAGTTTGA GTCGCTGCTT 
GTAGTGGGCA CCACCTTGCA CATCTACCCT GACCTTTACC ACTCGCAGTT CACCTACTTT 
CAGGTTTTGC GAGTGGTCCG TCTAATAAAA ATCTCCCCTG CACTAGAGGA CTTTGTGTAC 
AAGATCTTTG GTCCAGGGAA GAAACTGGGC AGTCTAGTGG TGTTTACTGC CAGTCTGCTC 
ATAGTGATGT CTGCCATCAG TCTGCAGATG TTCTGCTTTG TGGAGGATTT GGATCGTTTC 
ACCACATTTC CACGGGCGTT TATGTCCATG TTCCAGATTC TCACGCAGGA GGGCTGGGTG 
GATGTCATGG ACCAAACACT GGTGGCTGTG GGACAGATGT GGGCTCCGGT GGTGGCCATA 
TACTTTATTC TGTATCACCT TTTTGCCACA CTGATCCTTC TGAGTCTTTT TGTTGCTGTT 
ATTTTGGACA ATCTTGAGTT GGATGAAGAC CTGAAAAAGT TAAAACAGCT GAAGCAGAGT 
GAAGCCAATG CTGATACAAA AGAGAAACTT CCTTTGCGAC TGCGAATCTT TGAGAAGTTC 
CCCAATCGAC CGCAGATGGT GAAAATCTCC AAGCTGCCTT CAGACTTCAC TGTGCCCAGA 
ATCAGGGAGA GTTTCATGAA GCAGTTCATC GACCGACAGC AGCAGGACCC TAGCTGTCTG 
TTCCGTCGCC TTCCCTCAGC ATCATCCTCG TCTTGCGACC ATTCCAAACG CTCTGCCATT 
GAGGATAATA AATACATAGA TCAGAAGCTA CGAAAGTCAG TATTTAGCAT CCGGGCTCGC 
AACCTAATGG AAAAGGAAAT CACTGTCAAC AAAATTTTGC GAGCCTGCAC CAGGCAGCGC 
ATGCTGAGTG GCTCATTTGA GGGTCAGCCA ACTAAAGAGA GATCCATTCT CAGTGTCCAG 
CATCACATCC GCCAAGAACG CCGGTCCCTC AGACATGGCT CTACCAGCCA GAGAATCAGC 
AGAGGGAAAT CCTTGGAAAC ACTCACTCAG GACCACTCCA GCACGGTCCG CTACCGTAAT 
GCCCAGCGGG AGGACAGTGA AATCAAGATG ATACAAGAGA AGAAGGAGCA GGCTGAGATG 
AAACGAAAAG TCCAAGAGGA GGAACTTCGG GAAAACCATC CATACTTTGA CAAGCCACTC 
TTCATCGTTG GTAGAGAGCA TCGATTCAGA AACTTTTGCC GAATGATTGT TAGAGCCCGT 
TTTAATGCGT CAAATACGGA TCCCAATACA GGCGCAGTGA ACAGCACAAA ATACCATCAA 
TTATATGATT TGCTGGGCTT GGTAACCTAT CTGGACTGGG TGATGATCGT GGTCACTATC 
TGCTCTTGTA TTTCCATGAT GTTTGAGTCT CCTTTTACTA GAGTCATGCA TGTCCCAACC 
CTGCAGATTG GAGAATATGT GTTCGTCATC TTTATGAGCA TTGAGCTGAA CCTGAAGATT 
ATGGCAGATG GCCTGTTCTT CACTCCCACT GCAGTCATCC GAGACTTTGG CGGCGTCATG 
GACATCTTCA TCTACTTAGT AAGTTTGATC TTTTTATGCT GGCTGCCAAA TAATGTGCCC 
CCTGAGTCTG GTGCCCAGCT CCTCATGATG TTACGTTGCT TCCGGCCACT GAGAATCTTT 
AAACTGGTGC CCCAAATGAG GAAGGTGGTT CGAGAGGTCC TCAAGGGTTT CAAAGAGATT 
TTTCTGGTCT CTATTCTTCT CCTGACACTA ATGCTGGTGT TCGCAACCTT CGGGGTGCAA 
CTCTTTGCTG GAAAACTTGC CAAATGTAAT GATCCTCATA TTTCTAGCAA GGATGACTGT 
CACGGCATCT TTAGAATCAA TGTGAGCATT TCAAAGAACC TGAATTTGAA GCTCAAGGAT 
GGGGAGAAGA AACCAGGATT TTGGGTGCCA AGAGTTTGGG CAAACCCTCG AAACTTCAAT 
TTTGATAATG TGGGCAATGC CATGCTGGCT CTGTTTGAAG TGTTGTCGCT GAAGGGTTGG 
GTGGAGGTGA GAGATGTCAT CATTCACAGA GTAGGACCGA TTCATGGTAT CTACATCCAT 
GTCTTTGTGT TCTTGGGATG TATGATCGGA CTCACCCTTT TTGTTGGCGT TGTCATTGCC 
AACTTTAATG AAAATAAGGG CACTGCTCTG TTGACTGTGG ATCAGAGGAG ATGGGAAGAT 
TTAAAGAGTC GACTCAAGAT TGCTCAGCCC CTCCACCTCC CTCCACGTCC AGAAAATGGT 
GGATTTCGTG CAAAGATGTA TGACATCACC CAACATCCCT TTTTCAAGCG AGGGATTGCT 
GTGCTGGTTT TAGCACAGTC TGTCCTCCTG TCGGTTAAGT GGGATGAGCT TGGCGCAGTT 
ACGTTTCCCT TGGCCACAAT GTCAGTGGTT TTCACTTTCA TTTTTGTTTT GGAGGTGACT 
ATGAAGCTGA TAGCCATGTC ACCAGCTGGA TACTGGCAGA GCAGGAGGAA CAGATATGAC 
CTACTCGTCA CATCCCTCGG GCTCATATGG ATCATTCTGC ACTTCGCTTT ACAGAATGCT 
TACACATACA TGATGGGCAC CTGTGTGATA GTATTCAGAT TTTTCACCAT ATGCGGAAAG 
CATGTGACGT TAAAGATGCT TCTGCTTACT GTGGTCGTCA GTATGTACAA GAGTTTCTTC 
ATTATCGTGG GCATGTTTTT GCTGCTGCTG TGCTACGCTT TCGCCGGCGT CGTTCTTTTT 
GGCACCGTCA AATACGGAGA GAACATTAAC AGACACGCAA ACTTCTCCAC CGCAGGGAAA 
GCCATCACCG TACTTATTCG AATAGTCACA GGTGAAGACT GGAATAAGAT CATGCATGAC 
TGCATGGTGC AGCCGCCGTT CTGCACCCCA GACAAACATC GCTACTGGGA GACGGACTGC 
GGCAACTACG CTGGAGCCCT CATTTACTTC TGCTCCTTCT ATGTCATCAT CGCTTACATC 
ATGCTCAACC TGCTCGTAGC CATCATTGTG GAGAATTTCT CTTTGTTCTA CTCCACCGAA 
GAGGACCAGT TGCTAAGCTA CAACGACCTG CGTCACTTCC AGATCATCTG GAACATGGTG 
GATGACAAGC GGGAGGGAGT GATTCCGACC TCTCGGGTGA AGTTTCTGCT CCGTCTGCTG 
AGAGGTCGTC TGGAAGTGGA TCTGGACAAA GACAAGCTTC TCTTTAAACA CATGTGTTAT 
GAGATGGAGC GTCTGCACAA TGGAGGAGAC GTCACCTTTC ATGATGTACT GAGCATGCTG 
TCGTATCGTT CAGTTGATAT CCGCAAGAGT CTTCAGCTGG AGGAGCTGTT GGCAAGAGAG 
CAGCTTGAAT ACACTATAGA GGAGGAAGTG GCCAAACAAA CCATCCGCAT GTGGTTGAAG 
AAGTGCCTGA AACGCATCAG AGCCAAACAG CAGCAGTCAT GCAGCATCAT CCTCAGTCTG 
AGAGAGAGTC AACAGCAAGA CCTGCGGCGG CTGCTCAACC CCCCCAGCAT CGAGACCACC 
GTCCCCAGCG AAGACACCAA CACACACAAC CAGGACAATC CCACCCAGCC TGAGCAGAAC 
AGCGGCCTGC AGACGTTACT GAGCCCCACT CTGTCAGACC GCAGTGGATA CAGACAGGAC 
TCTGCTGACC GACCCCAGAG GAAGCTGGGA CAGTGGAGAC TTCCTGCAGG CCGCACAAGC 
GTGAAGTCCA TGGTGTGTAA GATGAACCCG GTGACTGACG AGGCGTCCTC AGGCTCTGAA 
GTTAAGAAGT GGTGGACGCG TCAACTGACT GTGGAAAGTG ATGAAAGTGG AGACGACCTC 
ATTGATATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167755.1
CDS	234..5465
Translation	MCSAGAISALLFPLIHSLYSTASPSMLKRKQSSRVEAQPMTDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIKGENSYVKDRWCMFDGFMVVFLWVSLVLQVFEIAELVDQMSPWGMLRIPRALIMIRVFRIYFRFELPRSRITNILKRSGEQIWSVTIFLLFFLLLYGILGVQMFGTFNHHCVTNETEKEKVTWNSLAIPDTHCSPNGEGYQCPVGFKCVDLEDYGLSRQELGYSGFNELGTSIFTVYEAASQEGWVFIMYRAIDSFPRWRSYFYFLTLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDASGGWQLVAVDVNKPHGRAPACLQQLMRSSVFHMFILSMVAVDVIVAASNYHRGDHKLTNDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFESLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEDLDRFTTFPRAFMSMFQILTQEGWVDVMDQTLVAVGQMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPRIRESFMKQFIDRQQQDPSCLFRRLPSASSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLMEKEITVNKILRACTRQRMLSGSFEGQPTKERSILSVQHHIRQERRSLRHGSTSQRISRGKSLETLTQDHSSTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRMIVRARFNASNTDPNTGAVNSTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFTRVMHVPTLQIGEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWLPNNVPPESGAQLLMMLRCFRPLRIFKLVPQMRKVVREVLKGFKEIFLVSILLLTLMLVFATFGVQLFAGKLAKCNDPHISSKDDCHGIFRINVSISKNLNLKLKDGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPENGGFRAKMYDITQHPFFKRGIAVLVLAQSVLLSVKWDELGAVTFPLATMSVVFTFIFVLEVTMKLIAMSPAGYWQSRRNRYDLLVTSLGLIWIILHFALQNAYTYMMGTCVIVFRFFTICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLIRIVTGEDWNKIMHDCMVQPPFCTPDKHRYWETDCGNYAGALIYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTSRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIILSLRESQQQDLRRLLNPPSIETTVPSEDTNTHNQDNPTQPEQNSGLQTLLSPTLSDRSGYRQDSADRPQRKLGQWRLPAGRTSVKSMVCKMNPVTDEASSGSEVKKWWTRQLTVESDESGDDLIDI

Target ORF information:

RefSeq Version	XM_005167698.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167698.4

ORF Insert Sequence:

ATGTGCAGTG CTGGAGCAAT TTCAGCTTTA CTTTTCCCGT TGATCCACTC TCTCTATAGC 
ACAGCGAGCC CAAGCATGCT GAAGAGGAAG CAGAGCTCGC GGGTCGAGGC CCAGCCTATG 
ACTGACTTTG GGCCAGACGA AACACTGACA GACAGTGCTG ACATTCTTTG GATCAATAAA 
CCATGGGTTC ACTCTCTCCT GCGGGCCTGT GCCATAATCA GTGTTATTTC GGTTTGTATG 
AATACACCTA AGACCTTTGA GCACTACCCT CCCCTACAGT ATGTCACCTT CGCCCTGGAC 
ACACTGCTGA TGTTCCTGTA CACCGCAGAG ATGATCGCCA AAATGCATAT CAGGGGCATC 
ATCAAGGGAG AAAACTCCTA TGTTAAAGAC CGCTGGTGCA TGTTTGATGG CTTCATGGTG 
GTCTTCCTCT GGGTCTCTCT TGTGCTTCAG GTGTTTGAAA TAGCGGAGCT CGTGGACCAA 
ATGTCACCCT GGGGAATGTT ACGCATACCG AGGGCCCTTA TTATGATCCG AGTCTTCCGA 
ATCTATTTCC GATTTGAATT GCCACGTAGC CGGATAACCA ACATTCTCAA GCGCTCAGGG 
GAGCAGATCT GGAGTGTGAC CATATTTTTG CTATTCTTCC TTCTTCTTTA TGGAATCCTG 
GGGGTTCAGA TGTTTGGCAC ATTCAATCAT CATTGCGTCA CCAATGAAAC AGAAAAAGAG 
AAAGTGACAT GGAACAGCCT AGCAATCCCG GATACACACT GCTCTCCGAA CGGGGAGGGA 
TATCAGTGTC CAGTGGGCTT TAAGTGTGTG GATCTGGAAG ATTATGGACT CAGTAGACAA 
GAACTTGGAT ACAGTGGATT CAATGAGCTT GGCACCAGTA TATTCACTGT ATATGAGGCT 
GCGTCTCAGG AGGGCTGGGT CTTCATCATG TATCGAGCCA TCGACAGCTT CCCACGCTGG 
CGCTCATATT TCTACTTCCT CACGCTCATC TTCTTCCTCG CCTGGCTGGT CAAGAATGTG 
TTCATTGCTG TGATTATCGA AACGTTTGCT GAGATCAGAG TGCAGTTCCA GCAGATGTGG 
GGATCCCGGA GCAGCACCAC GTCAACAGCT ACTACACAGA TGTTTCACGA GGACGCGTCT 
GGAGGATGGC AGCTCGTGGC GGTGGACGTG AATAAACCAC ATGGCCGGGC GCCTGCTTGC 
CTCCAGCAAC TGATGCGATC TTCAGTTTTC CACATGTTCA TCCTCAGTAT GGTGGCTGTT 
GATGTGATTG TAGCTGCGAG CAATTACCAC AGAGGTGACC ATAAACTCAC CAATGATGAG 
TTCTACCTGG CTGAGGTCGC CTTCACTGTG CTGTTTGATT TGGAGGCTCT GCTGAAAATC 
TGGTGTTTGG GCTTCACTGG ATACATCAGT TCATCGCTGC ACAAGTTTGA GTCGCTGCTT 
GTAGTGGGCA CCACCTTGCA CATCTACCCT GACCTTTACC ACTCGCAGTT CACCTACTTT 
CAGGTTTTGC GAGTGGTCCG TCTAATAAAA ATCTCCCCTG CACTAGAGGA CTTTGTGTAC 
AAGATCTTTG GTCCAGGGAA GAAACTGGGC AGTCTAGTGG TGTTTACTGC CAGTCTGCTC 
ATAGTGATGT CTGCCATCAG TCTGCAGATG TTCTGCTTTG TGGAGGATTT GGATCGTTTC 
ACCACATTTC CACGGGCGTT TATGTCCATG TTCCAGATTC TCACGCAGGA GGGCTGGGTG 
GATGTCATGG ACCAAACACT GGTGGCTGTG GGACAGATGT GGGCTCCGGT GGTGGCCATA 
TACTTTATTC TGTATCACCT TTTTGCCACA CTGATCCTTC TGAGTCTTTT TGTTGCTGTT 
ATTTTGGACA ATCTTGAGTT GGATGAAGAC CTGAAAAAGT TAAAACAGCT GAAGCAGAGT 
GAAGCCAATG CTGATACAAA AGAGAAACTT CCTTTGCGAC TGCGAATCTT TGAGAAGTTC 
CCCAATCGAC CGCAGATGGT GAAAATCTCC AAGCTGCCTT CAGACTTCAC TGTGCCCAGA 
ATCAGGGAGA GTTTCATGAA GCAGTTCATC GACCGACAGC AGCAGGACCC TAGCTGTCTG 
TTCCGTCGCC TTCCCTCAGC ATCATCCTCG TCTTGCGACC ATTCCAAACG CTCTGCCATT 
GAGGATAATA AATACATAGA TCAGAAGCTA CGAAAGTCAG TATTTAGCAT CCGGGCTCGC 
AACCTAATGG AAAAGGAAAT CACTGTCAAC AAAATTTTGC GAGCCTGCAC CAGGCAGCGC 
ATGCTGAGTG GCTCATTTGA GGGTCAGCCA ACTAAAGAGA GATCCATTCT CAGTGTCCAG 
CATCACATCC GCCAAGAACG CCGGTCCCTC AGACATGGCT CTACCAGCCA GAGAATCAGC 
AGAGGGAAAT CCTTGGAAAC ACTCACTCAG GACCACTCCA GCACGGTCCG CTACCGTAAT 
GCCCAGCGGG AGGACAGTGA AATCAAGATG ATACAAGAGA AGAAGGAGCA GGCTGAGATG 
AAACGAAAAG TCCAAGAGGA GGAACTTCGG GAAAACCATC CATACTTTGA CAAGCCACTC 
TTCATCGTTG GTAGAGAGCA TCGATTCAGA AACTTTTGCC GAATGATTGT TAGAGCCCGT 
TTTAATGCGT CAAATACGGA TCCCAATACA GGCGCAGTGA ACAGCACAAA ATACCATCAA 
TTATATGATT TGCTGGGCTT GGTAACCTAT CTGGACTGGG TGATGATCGT GGTCACTATC 
TGCTCTTGTA TTTCCATGAT GTTTGAGTCT CCTTTTACTA GAGTCATGCA TGTCCCAACC 
CTGCAGATTG GAGAATATGT GTTCGTCATC TTTATGAGCA TTGAGCTGAA CCTGAAGATT 
ATGGCAGATG GCCTGTTCTT CACTCCCACT GCAGTCATCC GAGACTTTGG CGGCGTCATG 
GACATCTTCA TCTACTTAGT AAGTTTGATC TTTTTATGCT GGCTGCCAAA TAATGTGCCC 
CCTGAGTCTG GTGCCCAGCT CCTCATGATG TTACGTTGCT TCCGGCCACT GAGAATCTTT 
AAACTGGTGC CCCAAATGAG GAAGGTGGTT CGAGAGGTCC TCAAGGGTTT CAAAGAGATT 
TTTCTGGTCT CTATTCTTCT CCTGACACTA ATGCTGGTGT TCGCAACCTT CGGGGTGCAA 
CTCTTTGCTG GAAAACTTGC CAAATGTAAT GATCCTCATA TTTCTAGCAA GGATGACTGT 
CACGGCATCT TTAGAATCAA TGTGAGCATT TCAAAGAACC TGAATTTGAA GCTCAAGGAT 
GGGGAGAAGA AACCAGGATT TTGGGTGCCA AGAGTTTGGG CAAACCCTCG AAACTTCAAT 
TTTGATAATG TGGGCAATGC CATGCTGGCT CTGTTTGAAG TGTTGTCGCT GAAGGGTTGG 
GTGGAGGTGA GAGATGTCAT CATTCACAGA GTAGGACCGA TTCATGGTAT CTACATCCAT 
GTCTTTGTGT TCTTGGGATG TATGATCGGA CTCACCCTTT TTGTTGGCGT TGTCATTGCC 
AACTTTAATG AAAATAAGGG CACTGCTCTG TTGACTGTGG ATCAGAGGAG ATGGGAAGAT 
TTAAAGAGTC GACTCAAGAT TGCTCAGCCC CTCCACCTCC CTCCACGTCC AGAAAATGGT 
GGATTTCGTG CAAAGATGTA TGACATCACC CAACATCCCT TTTTCAAGCG AGGGATTGCT 
GTGCTGGTTT TAGCACAGTC TGTCCTCCTG TCGGTTAAGT GGGATGAGCT TGGCGCAGTT 
ACGTTTCCCT TGGCCACAAT GTCAGTGGTT TTCACTTTCA TTTTTGTTTT GGAGGTGACT 
ATGAAGCTGA TAGCCATGTC ACCAGCTGGA TACTGGCAGA GCAGGAGGAA CAGATATGAC 
CTACTCGTCA CATCCCTCGG GCTCATATGG ATCATTCTGC ACTTCGCTTT ACAGAATGCT 
TACACATACA TGATGGGCAC CTGTGTGATA GTATTCAGAT TTTTCACCAT ATGCGGAAAG 
CATGTGACGT TAAAGATGCT TCTGCTTACT GTGGTCGTCA GTATGTACAA GAGTTTCTTC 
ATTATCGTGG GCATGTTTTT GCTGCTGCTG TGCTACGCTT TCGCCGGCGT CGTTCTTTTT 
GGCACCGTCA AATACGGAGA GAACATTAAC AGACACGCAA ACTTCTCCAC CGCAGGGAAA 
GCCATCACCG TACTTATTCG AATAGTCACA GGTGAAGACT GGAATAAGAT CATGCATGAC 
TGCATGGTGC AGCCGCCGTT CTGCACCCCA GACAAACATC GCTACTGGGA GACGGACTGC 
GGCAACTACG CTGGAGCCCT CATTTACTTC TGCTCCTTCT ATGTCATCAT CGCTTACATC 
ATGCTCAACC TGCTCGTAGC CATCATTGTG GAGAATTTCT CTTTGTTCTA CTCCACCGAA 
GAGGACCAGT TGCTAAGCTA CAACGACCTG CGTCACTTCC AGATCATCTG GAACATGGTG 
GATGACAAGC GGGAGGGAGT GATTCCGACC TCTCGGGTGA AGTTTCTGCT CCGTCTGCTG 
AGAGGTCGTC TGGAAGTGGA TCTGGACAAA GACAAGCTTC TCTTTAAACA CATGTGTTAT 
GAGATGGAGC GTCTGCACAA TGGAGGAGAC GTCACCTTTC ATGATGTACT GAGCATGCTG 
TCGTATCGTT CAGTTGATAT CCGCAAGAGT CTTCAGCTGG AGGAGCTGTT GGCAAGAGAG 
CAGCTTGAAT ACACTATAGA GGAGGAAGTG GCCAAACAAA CCATCCGCAT GTGGTTGAAG 
AAGTGCCTGA AACGCATCAG AGCCAAACAG CAGCAGTCAT GCAGCATCAT CCTCAGTCTG 
AGAGAGAGTC AACAGCAAGA CCTGCGGCGG CTGCTCAACC CCCCCAGCAT CGAGACCACC 
GTCCCCAGCG AAGACACCAA CACACACAAC CAGGACAATC CCACCCAGCC TGAGCAGAAC 
AGCGGCCTGC AGACGTTACT GAGCCCCACT CTGTCAGACC GCAGTGGATA CAGACAGGAC 
TCTGCTGACC GACCCCAGAG GAAGCTGGGA CAGTGGAGAC TTCCTGCAGG CCGCACAAGC 
GTGAAGTCCA TGGTGTGTAA GATGAACCCG GTGACTGACG AGGCGTCCTC AGGCTCTGAA 
GTTAAGAAGT GGTGGACGCG TCAACTGACT GTGGAAAGTG ATGAAAGTGG AGACGACCTC 
ATTGATATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_009304740.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303015.1 Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X4, mRNA.

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CloneID	ODa41705
Clone ID Related Accession (Same CDS sequence)	XM_009304740.2 , XM_009304739.2 , XM_009304740.3
Accession Version	XM_009304740.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sodium leak channel non-selective protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGA TGAATGCACC AATGAGGAGC CTGACCATCC ACTCTCTCTA TAGCACAGCG 
AGCCCAAGCA TGCTGAAGAG GAAGCAGAGC TCGCGGGTCG AGGCCCAGCC TATGACTGAC 
TTTGGGCCAG ACGAAACACT GACAGACAGT GCTGACATTC TTTGGATCAA TAAACCATGG 
GTTCACTCTC TCCTGCGGGC CTGTGCCATA ATCAGTGTTA TTTCGGTTTG TATGAATACA 
CCTAAGACCT TTGAGCACTA CCCTCCCCTA CAGTATGTCA CCTTCGCCCT GGACACACTG 
CTGATGTTCC TGTACACCGC AGAGATGATC GCCAAAATGC ATATCAGGGG CATCATCAAG 
GGAGAAAACT CCTATGTTAA AGACCGCTGG TGCATGTTTG ATGGCTTCAT GGTGGTCTTC 
CTCTGGGTCT CTCTTGTGCT TCAGGTGTTT GAAATAGCGG AGCTCGTGGA CCAAATGTCA 
CCCTGGGGAA TGTTACGCAT ACCGAGGGCC CTTATTATGA TCCGAGTCTT CCGAATCTAT 
TTCCGATTTG AATTGCCACG TAGCCGGATA ACCAACATTC TCAAGCGCTC AGGGGAGCAG 
ATCTGGAGTG TGACCATATT TTTGCTATTC TTCCTTCTTC TTTATGGAAT CCTGGGGGTT 
CAGATGTTTG GCACATTCAA TCATCATTGC GTCACCAATG AAACAGAAAA AGAGAAAGTG 
ACATGGAACA GCCTAGCAAT CCCGGATACA CACTGCTCTC CGAACGGGGA GGGATATCAG 
TGTCCAGTGG GCTTTAAGTG TGTGGATCTG GAAGATTATG GACTCAGTAG ACAAGAACTT 
GGATACAGTG GATTCAATGA GCTTGGCACC AGTATATTCA CTGTATATGA GGCTGCGTCT 
CAGGAGGGCT GGGTCTTCAT CATGTATCGA GCCATCGACA GCTTCCCACG CTGGCGCTCA 
TATTTCTACT TCCTCACGCT CATCTTCTTC CTCGCCTGGC TGGTCAAGAA TGTGTTCATT 
GCTGTGATTA TCGAAACGTT TGCTGAGATC AGAGTGCAGT TCCAGCAGAT GTGGGGATCC 
CGGAGCAGCA CCACGTCAAC AGCTACTACA CAGATGTTTC ACGAGGACGC GTCTGGAGGA 
TGGCAGCTCG TGGCGGTGGA CGTGAATAAA CCACATGGCC GGGCGCCTGC TTGCCTCCAG 
CAACTGATGC GATCTTCAGT TTTCCACATG TTCATCCTCA GTATGGTGGC TGTTGATGTG 
ATTGTAGCTG CGAGCAATTA CCACAGAGGT GACCATAAAC TCACCAATGA TGAGTTCTAC 
CTGGCTGAGG TCGCCTTCAC TGTGCTGTTT GATTTGGAGG CTCTGCTGAA AATCTGGTGT 
TTGGGCTTCA CTGGATACAT CAGTTCATCG CTGCACAAGT TTGAGTCGCT GCTTGTAGTG 
GGCACCACCT TGCACATCTA CCCTGACCTT TACCACTCGC AGTTCACCTA CTTTCAGGTT 
TTGCGAGTGG TCCGTCTAAT AAAAATCTCC CCTGCACTAG AGGACTTTGT GTACAAGATC 
TTTGGTCCAG GGAAGAAACT GGGCAGTCTA GTGGTGTTTA CTGCCAGTCT GCTCATAGTG 
ATGTCTGCCA TCAGTCTGCA GATGTTCTGC TTTGTGGAGG ATTTGGATCG TTTCACCACA 
TTTCCACGGG CGTTTATGTC CATGTTCCAG ATTCTCACGC AGGAGGGCTG GGTGGATGTC 
ATGGACCAAA CACTGGTGGC TGTGGGACAG ATGTGGGCTC CGGTGGTGGC CATATACTTT 
ATTCTGTATC ACCTTTTTGC CACACTGATC CTTCTGAGTC TTTTTGTTGC TGTTATTTTG 
GACAATCTTG AGTTGGATGA AGACCTGAAA AAGTTAAAAC AGCTGAAGCA GAGTGAAGCC 
AATGCTGATA CAAAAGAGAA ACTTCCTTTG CGACTGCGAA TCTTTGAGAA GTTCCCCAAT 
CGACCGCAGA TGGTGAAAAT CTCCAAGCTG CCTTCAGACT TCACTGTGCC CAGAATCAGG 
GAGAGTTTCA TGAAGCAGTT CATCGACCGA CAGCAGCAGG ACCCTAGCTG TCTGTTCCGT 
CGCCTTCCCT CAGCATCATC CTCGTCTTGC GACCATTCCA AACGCTCTGC CATTGAGGAT 
AATAAATACA TAGATCAGAA GCTACGAAAG TCAGTATTTA GCATCCGGGC TCGCAACCTA 
ATGGAAAAGG AAATCACTGT CAACAAAATT TTGCGAGCCT GCACCAGGCA GCGCATGCTG 
AGTGGCTCAT TTGAGGGTCA GCCAACTAAA GAGAGATCCA TTCTCAGTGT CCAGCATCAC 
ATCCGCCAAG AACGCCGGTC CCTCAGACAT GGCTCTACCA GCCAGAGAAT CAGCAGAGGG 
AAATCCTTGG AAACACTCAC TCAGGACCAC TCCAGCACGG TCCGCTACCG TAATGCCCAG 
CGGGAGGACA GTGAAATCAA GATGATACAA GAGAAGAAGG AGCAGGCTGA GATGAAACGA 
AAAGTCCAAG AGGAGGAACT TCGGGAAAAC CATCCATACT TTGACAAGCC ACTCTTCATC 
GTTGGTAGAG AGCATCGATT CAGAAACTTT TGCCGAATGA TTGTTAGAGC CCGTTTTAAT 
GCGTCAAATA CGGATCCCAA TACAGGCGCA GTGAACAGCA CAAAATACCA TCAATTATAT 
GATTTGCTGG GCTTGGTAAC CTATCTGGAC TGGGTGATGA TCGTGGTCAC TATCTGCTCT 
TGTATTTCCA TGATGTTTGA GTCTCCTTTT ACTAGAGTCA TGCATGTCCC AACCCTGCAG 
ATTGGAGAAT ATGTGTTCGT CATCTTTATG AGCATTGAGC TGAACCTGAA GATTATGGCA 
GATGGCCTGT TCTTCACTCC CACTGCAGTC ATCCGAGACT TTGGCGGCGT CATGGACATC 
TTCATCTACT TAGTAAGTTT GATCTTTTTA TGCTGGCTGC CAAATAATGT GCCCCCTGAG 
TCTGGTGCCC AGCTCCTCAT GATGTTACGT TGCTTCCGGC CACTGAGAAT CTTTAAACTG 
GTGCCCCAAA TGAGGAAGGT GGTTCGAGAG GTCCTCAAGG GTTTCAAAGA GATTTTTCTG 
GTCTCTATTC TTCTCCTGAC ACTAATGCTG GTGTTCGCAA CCTTCGGGGT GCAACTCTTT 
GCTGGAAAAC TTGCCAAATG TAATGATCCT CATATTTCTA GCAAGGATGA CTGTCACGGC 
ATCTTTAGAA TCAATGTGAG CATTTCAAAG AACCTGAATT TGAAGCTCAA GGATGGGGAG 
AAGAAACCAG GATTTTGGGT GCCAAGAGTT TGGGCAAACC CTCGAAACTT CAATTTTGAT 
AATGTGGGCA ATGCCATGCT GGCTCTGTTT GAAGTGTTGT CGCTGAAGGG TTGGGTGGAG 
GTGAGAGATG TCATCATTCA CAGAGTAGGA CCGATTCATG GTATCTACAT CCATGTCTTT 
GTGTTCTTGG GATGTATGAT CGGACTCACC CTTTTTGTTG GCGTTGTCAT TGCCAACTTT 
AATGAAAATA AGGGCACTGC TCTGTTGACT GTGGATCAGA GGAGATGGGA AGATTTAAAG 
AGTCGACTCA AGATTGCTCA GCCCCTCCAC CTCCCTCCAC GTCCAGAAAA TGGTGGATTT 
CGTGCAAAGA TGTATGACAT CACCCAACAT CCCTTTTTCA AGCGAGGGAT TGCTGTGCTG 
GTTTTAGCAC AGTCTGTCCT CCTGTCGGTT AAGTGGGATG AGCTTGGCGC AGTTACGTTT 
CCCTTGGCCA CAATGTCAGT GGTTTTCACT TTCATTTTTG TTTTGGAGGT GACTATGAAG 
CTGATAGCCA TGTCACCAGC TGGATACTGG CAGAGCAGGA GGAACAGATA TGACCTACTC 
GTCACATCCC TCGGGCTCAT ATGGATCATT CTGCACTTCG CTTTACAGAA TGCTTACACA 
TACATGATGG GCACCTGTGT GATAGTATTC AGATTTTTCA CCATATGCGG AAAGCATGTG 
ACGTTAAAGA TGCTTCTGCT TACTGTGGTC GTCAGTATGT ACAAGAGTTT CTTCATTATC 
GTGGGCATGT TTTTGCTGCT GCTGTGCTAC GCTTTCGCCG GCGTCGTTCT TTTTGGCACC 
GTCAAATACG GAGAGAACAT TAACAGACAC GCAAACTTCT CCACCGCAGG GAAAGCCATC 
ACCGTACTTA TTCGAATAGT CACAGGTGAA GACTGGAATA AGATCATGCA TGACTGCATG 
GTGCAGCCGC CGTTCTGCAC CCCAGACAAA CATCGCTACT GGGAGACGGA CTGCGGCAAC 
TACGCTGGAG CCCTCATTTA CTTCTGCTCC TTCTATGTCA TCATCGCTTA CATCATGCTC 
AACCTGCTCG TAGCCATCAT TGTGGAGAAT TTCTCTTTGT TCTACTCCAC CGAAGAGGAC 
CAGTTGCTAA GCTACAACGA CCTGCGTCAC TTCCAGATCA TCTGGAACAT GGTGGATGAC 
AAGCGGGAGG GAGTGATTCC GACCTCTCGG GTGAAGTTTC TGCTCCGTCT GCTGAGAGGT 
CGTCTGGAAG TGGATCTGGA CAAAGACAAG CTTCTCTTTA AACACATGTG TTATGAGATG 
GAGCGTCTGC ACAATGGAGG AGACGTCACC TTTCATGATG TACTGAGCAT GCTGTCGTAT 
CGTTCAGTTG ATATCCGCAA GAGTCTTCAG CTGGAGGAGC TGTTGGCAAG AGAGCAGCTT 
GAATACACTA TAGAGGAGGA AGTGGCCAAA CAAACCATCC GCATGTGGTT GAAGAAGTGC 
CTGAAACGCA TCAGAGCCAA ACAGCAGCAG TCATGCAGCA TCATCCTCAG TCTGAGAGAG 
AGTCAACAGC AAGACCTGCG GCGGCTGCTC AACCCCCCCA GCATCGAGAC CACCGTCCCC 
AGCGAAGACA CCAACACACA CAACCAGGAC AATCCCACCC AGCCTGAGCA GAACAGCGGC 
CTGCAGACGT TACTGAGCCC CACTCTGTCA GACCGCAGTG GATACAGACA GGACTCTGCT 
GACCGACCCC AGAGGAAGCT GGGACAGTGG AGACTTCCTG CAGGCCGCAC AAGCGTGAAG 
TCCATGGTGT GTAAGATGAA CCCGGTGACT GACGAGGCGT CCTCAGGCTC TGAAGTTAAG 
AAGTGGTGGA CGCGTCAACT GACTGTGGAA AGTGATGAAA GTGGAGACGA CCTCATTGAT 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303015.1
CDS	179..5404
Translation	MPGMNAPMRSLTIHSLYSTASPSMLKRKQSSRVEAQPMTDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIKGENSYVKDRWCMFDGFMVVFLWVSLVLQVFEIAELVDQMSPWGMLRIPRALIMIRVFRIYFRFELPRSRITNILKRSGEQIWSVTIFLLFFLLLYGILGVQMFGTFNHHCVTNETEKEKVTWNSLAIPDTHCSPNGEGYQCPVGFKCVDLEDYGLSRQELGYSGFNELGTSIFTVYEAASQEGWVFIMYRAIDSFPRWRSYFYFLTLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDASGGWQLVAVDVNKPHGRAPACLQQLMRSSVFHMFILSMVAVDVIVAASNYHRGDHKLTNDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFESLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEDLDRFTTFPRAFMSMFQILTQEGWVDVMDQTLVAVGQMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPRIRESFMKQFIDRQQQDPSCLFRRLPSASSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLMEKEITVNKILRACTRQRMLSGSFEGQPTKERSILSVQHHIRQERRSLRHGSTSQRISRGKSLETLTQDHSSTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRMIVRARFNASNTDPNTGAVNSTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFTRVMHVPTLQIGEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWLPNNVPPESGAQLLMMLRCFRPLRIFKLVPQMRKVVREVLKGFKEIFLVSILLLTLMLVFATFGVQLFAGKLAKCNDPHISSKDDCHGIFRINVSISKNLNLKLKDGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPENGGFRAKMYDITQHPFFKRGIAVLVLAQSVLLSVKWDELGAVTFPLATMSVVFTFIFVLEVTMKLIAMSPAGYWQSRRNRYDLLVTSLGLIWIILHFALQNAYTYMMGTCVIVFRFFTICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLIRIVTGEDWNKIMHDCMVQPPFCTPDKHRYWETDCGNYAGALIYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTSRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIILSLRESQQQDLRRLLNPPSIETTVPSEDTNTHNQDNPTQPEQNSGLQTLLSPTLSDRSGYRQDSADRPQRKLGQWRLPAGRTSVKSMVCKMNPVTDEASSGSEVKKWWTRQLTVESDESGDDLIDI

Target ORF information:

RefSeq Version	XM_009304740.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304740.2

ORF Insert Sequence:

ATGCCGGGGA TGAATGCACC AATGAGGAGC CTGACCATCC ACTCTCTCTA TAGCACAGCG 
AGCCCAAGCA TGCTGAAGAG GAAGCAGAGC TCGCGGGTCG AGGCCCAGCC TATGACTGAC 
TTTGGGCCAG ACGAAACACT GACAGACAGT GCTGACATTC TTTGGATCAA TAAACCATGG 
GTTCACTCTC TCCTGCGGGC CTGTGCCATA ATCAGTGTTA TTTCGGTTTG TATGAATACA 
CCTAAGACCT TTGAGCACTA CCCTCCCCTA CAGTATGTCA CCTTCGCCCT GGACACACTG 
CTGATGTTCC TGTACACCGC AGAGATGATC GCCAAAATGC ATATCAGGGG CATCATCAAG 
GGAGAAAACT CCTATGTTAA AGACCGCTGG TGCATGTTTG ATGGCTTCAT GGTGGTCTTC 
CTCTGGGTCT CTCTTGTGCT TCAGGTGTTT GAAATAGCGG AGCTCGTGGA CCAAATGTCA 
CCCTGGGGAA TGTTACGCAT ACCGAGGGCC CTTATTATGA TCCGAGTCTT CCGAATCTAT 
TTCCGATTTG AATTGCCACG TAGCCGGATA ACCAACATTC TCAAGCGCTC AGGGGAGCAG 
ATCTGGAGTG TGACCATATT TTTGCTATTC TTCCTTCTTC TTTATGGAAT CCTGGGGGTT 
CAGATGTTTG GCACATTCAA TCATCATTGC GTCACCAATG AAACAGAAAA AGAGAAAGTG 
ACATGGAACA GCCTAGCAAT CCCGGATACA CACTGCTCTC CGAACGGGGA GGGATATCAG 
TGTCCAGTGG GCTTTAAGTG TGTGGATCTG GAAGATTATG GACTCAGTAG ACAAGAACTT 
GGATACAGTG GATTCAATGA GCTTGGCACC AGTATATTCA CTGTATATGA GGCTGCGTCT 
CAGGAGGGCT GGGTCTTCAT CATGTATCGA GCCATCGACA GCTTCCCACG CTGGCGCTCA 
TATTTCTACT TCCTCACGCT CATCTTCTTC CTCGCCTGGC TGGTCAAGAA TGTGTTCATT 
GCTGTGATTA TCGAAACGTT TGCTGAGATC AGAGTGCAGT TCCAGCAGAT GTGGGGATCC 
CGGAGCAGCA CCACGTCAAC AGCTACTACA CAGATGTTTC ACGAGGACGC GTCTGGAGGA 
TGGCAGCTCG TGGCGGTGGA CGTGAATAAA CCACATGGCC GGGCGCCTGC TTGCCTCCAG 
CAACTGATGC GATCTTCAGT TTTCCACATG TTCATCCTCA GTATGGTGGC TGTTGATGTG 
ATTGTAGCTG CGAGCAATTA CCACAGAGGT GACCATAAAC TCACCAATGA TGAGTTCTAC 
CTGGCTGAGG TCGCCTTCAC TGTGCTGTTT GATTTGGAGG CTCTGCTGAA AATCTGGTGT 
TTGGGCTTCA CTGGATACAT CAGTTCATCG CTGCACAAGT TTGAGTCGCT GCTTGTAGTG 
GGCACCACCT TGCACATCTA CCCTGACCTT TACCACTCGC AGTTCACCTA CTTTCAGGTT 
TTGCGAGTGG TCCGTCTAAT AAAAATCTCC CCTGCACTAG AGGACTTTGT GTACAAGATC 
TTTGGTCCAG GGAAGAAACT GGGCAGTCTA GTGGTGTTTA CTGCCAGTCT GCTCATAGTG 
ATGTCTGCCA TCAGTCTGCA GATGTTCTGC TTTGTGGAGG ATTTGGATCG TTTCACCACA 
TTTCCACGGG CGTTTATGTC CATGTTCCAG ATTCTCACGC AGGAGGGCTG GGTGGATGTC 
ATGGACCAAA CACTGGTGGC TGTGGGACAG ATGTGGGCTC CGGTGGTGGC CATATACTTT 
ATTCTGTATC ACCTTTTTGC CACACTGATC CTTCTGAGTC TTTTTGTTGC TGTTATTTTG 
GACAATCTTG AGTTGGATGA AGACCTGAAA AAGTTAAAAC AGCTGAAGCA GAGTGAAGCC 
AATGCTGATA CAAAAGAGAA ACTTCCTTTG CGACTGCGAA TCTTTGAGAA GTTCCCCAAT 
CGACCGCAGA TGGTGAAAAT CTCCAAGCTG CCTTCAGACT TCACTGTGCC CAGAATCAGG 
GAGAGTTTCA TGAAGCAGTT CATCGACCGA CAGCAGCAGG ACCCTAGCTG TCTGTTCCGT 
CGCCTTCCCT CAGCATCATC CTCGTCTTGC GACCATTCCA AACGCTCTGC CATTGAGGAT 
AATAAATACA TAGATCAGAA GCTACGAAAG TCAGTATTTA GCATCCGGGC TCGCAACCTA 
ATGGAAAAGG AAATCACTGT CAACAAAATT TTGCGAGCCT GCACCAGGCA GCGCATGCTG 
AGTGGCTCAT TTGAGGGTCA GCCAACTAAA GAGAGATCCA TTCTCAGTGT CCAGCATCAC 
ATCCGCCAAG AACGCCGGTC CCTCAGACAT GGCTCTACCA GCCAGAGAAT CAGCAGAGGG 
AAATCCTTGG AAACACTCAC TCAGGACCAC TCCAGCACGG TCCGCTACCG TAATGCCCAG 
CGGGAGGACA GTGAAATCAA GATGATACAA GAGAAGAAGG AGCAGGCTGA GATGAAACGA 
AAAGTCCAAG AGGAGGAACT TCGGGAAAAC CATCCATACT TTGACAAGCC ACTCTTCATC 
GTTGGTAGAG AGCATCGATT CAGAAACTTT TGCCGAATGA TTGTTAGAGC CCGTTTTAAT 
GCGTCAAATA CGGATCCCAA TACAGGCGCA GTGAACAGCA CAAAATACCA TCAATTATAT 
GATTTGCTGG GCTTGGTAAC CTATCTGGAC TGGGTGATGA TCGTGGTCAC TATCTGCTCT 
TGTATTTCCA TGATGTTTGA GTCTCCTTTT ACTAGAGTCA TGCATGTCCC AACCCTGCAG 
ATTGGAGAAT ATGTGTTCGT CATCTTTATG AGCATTGAGC TGAACCTGAA GATTATGGCA 
GATGGCCTGT TCTTCACTCC CACTGCAGTC ATCCGAGACT TTGGCGGCGT CATGGACATC 
TTCATCTACT TAGTAAGTTT GATCTTTTTA TGCTGGCTGC CAAATAATGT GCCCCCTGAG 
TCTGGTGCCC AGCTCCTCAT GATGTTACGT TGCTTCCGGC CACTGAGAAT CTTTAAACTG 
GTGCCCCAAA TGAGGAAGGT GGTTCGAGAG GTCCTCAAGG GTTTCAAAGA GATTTTTCTG 
GTCTCTATTC TTCTCCTGAC ACTAATGCTG GTGTTCGCAA CCTTCGGGGT GCAACTCTTT 
GCTGGAAAAC TTGCCAAATG TAATGATCCT CATATTTCTA GCAAGGATGA CTGTCACGGC 
ATCTTTAGAA TCAATGTGAG CATTTCAAAG AACCTGAATT TGAAGCTCAA GGATGGGGAG 
AAGAAACCAG GATTTTGGGT GCCAAGAGTT TGGGCAAACC CTCGAAACTT CAATTTTGAT 
AATGTGGGCA ATGCCATGCT GGCTCTGTTT GAAGTGTTGT CGCTGAAGGG TTGGGTGGAG 
GTGAGAGATG TCATCATTCA CAGAGTAGGA CCGATTCATG GTATCTACAT CCATGTCTTT 
GTGTTCTTGG GATGTATGAT CGGACTCACC CTTTTTGTTG GCGTTGTCAT TGCCAACTTT 
AATGAAAATA AGGGCACTGC TCTGTTGACT GTGGATCAGA GGAGATGGGA AGATTTAAAG 
AGTCGACTCA AGATTGCTCA GCCCCTCCAC CTCCCTCCAC GTCCAGAAAA TGGTGGATTT 
CGTGCAAAGA TGTATGACAT CACCCAACAT CCCTTTTTCA AGCGAGGGAT TGCTGTGCTG 
GTTTTAGCAC AGTCTGTCCT CCTGTCGGTT AAGTGGGATG AGCTTGGCGC AGTTACGTTT 
CCCTTGGCCA CAATGTCAGT GGTTTTCACT TTCATTTTTG TTTTGGAGGT GACTATGAAG 
CTGATAGCCA TGTCACCAGC TGGATACTGG CAGAGCAGGA GGAACAGATA TGACCTACTC 
GTCACATCCC TCGGGCTCAT ATGGATCATT CTGCACTTCG CTTTACAGAA TGCTTACACA 
TACATGATGG GCACCTGTGT GATAGTATTC AGATTTTTCA CCATATGCGG AAAGCATGTG 
ACGTTAAAGA TGCTTCTGCT TACTGTGGTC GTCAGTATGT ACAAGAGTTT CTTCATTATC 
GTGGGCATGT TTTTGCTGCT GCTGTGCTAC GCTTTCGCCG GCGTCGTTCT TTTTGGCACC 
GTCAAATACG GAGAGAACAT TAACAGACAC GCAAACTTCT CCACCGCAGG GAAAGCCATC 
ACCGTACTTA TTCGAATAGT CACAGGTGAA GACTGGAATA AGATCATGCA TGACTGCATG 
GTGCAGCCGC CGTTCTGCAC CCCAGACAAA CATCGCTACT GGGAGACGGA CTGCGGCAAC 
TACGCTGGAG CCCTCATTTA CTTCTGCTCC TTCTATGTCA TCATCGCTTA CATCATGCTC 
AACCTGCTCG TAGCCATCAT TGTGGAGAAT TTCTCTTTGT TCTACTCCAC CGAAGAGGAC 
CAGTTGCTAA GCTACAACGA CCTGCGTCAC TTCCAGATCA TCTGGAACAT GGTGGATGAC 
AAGCGGGAGG GAGTGATTCC GACCTCTCGG GTGAAGTTTC TGCTCCGTCT GCTGAGAGGT 
CGTCTGGAAG TGGATCTGGA CAAAGACAAG CTTCTCTTTA AACACATGTG TTATGAGATG 
GAGCGTCTGC ACAATGGAGG AGACGTCACC TTTCATGATG TACTGAGCAT GCTGTCGTAT 
CGTTCAGTTG ATATCCGCAA GAGTCTTCAG CTGGAGGAGC TGTTGGCAAG AGAGCAGCTT 
GAATACACTA TAGAGGAGGA AGTGGCCAAA CAAACCATCC GCATGTGGTT GAAGAAGTGC 
CTGAAACGCA TCAGAGCCAA ACAGCAGCAG TCATGCAGCA TCATCCTCAG TCTGAGAGAG 
AGTCAACAGC AAGACCTGCG GCGGCTGCTC AACCCCCCCA GCATCGAGAC CACCGTCCCC 
AGCGAAGACA CCAACACACA CAACCAGGAC AATCCCACCC AGCCTGAGCA GAACAGCGGC 
CTGCAGACGT TACTGAGCCC CACTCTGTCA GACCGCAGTG GATACAGACA GGACTCTGCT 
GACCGACCCC AGAGGAAGCT GGGACAGTGG AGACTTCCTG CAGGCCGCAC AAGCGTGAAG 
TCCATGGTGT GTAAGATGAA CCCGGTGACT GACGAGGCGT CCTCAGGCTC TGAAGTTAAG 
AAGTGGTGGA CGCGTCAACT GACTGTGGAA AGTGATGAAA GTGGAGACGA CCTCATTGAT 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_009304739.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303014.1 Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X3, mRNA.

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CloneID	ODa41705
Clone ID Related Accession (Same CDS sequence)	XM_009304740.2 , XM_009304739.2 , XM_009304740.3
Accession Version	XM_009304739.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sodium leak channel non-selective protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGA TGAATGCACC AATGAGGAGC CTGACCATCC ACTCTCTCTA TAGCACAGCG 
AGCCCAAGCA TGCTGAAGAG GAAGCAGAGC TCGCGGGTCG AGGCCCAGCC TATGACTGAC 
TTTGGGCCAG ACGAAACACT GACAGACAGT GCTGACATTC TTTGGATCAA TAAACCATGG 
GTTCACTCTC TCCTGCGGGC CTGTGCCATA ATCAGTGTTA TTTCGGTTTG TATGAATACA 
CCTAAGACCT TTGAGCACTA CCCTCCCCTA CAGTATGTCA CCTTCGCCCT GGACACACTG 
CTGATGTTCC TGTACACCGC AGAGATGATC GCCAAAATGC ATATCAGGGG CATCATCAAG 
GGAGAAAACT CCTATGTTAA AGACCGCTGG TGCATGTTTG ATGGCTTCAT GGTGGTCTTC 
CTCTGGGTCT CTCTTGTGCT TCAGGTGTTT GAAATAGCGG AGCTCGTGGA CCAAATGTCA 
CCCTGGGGAA TGTTACGCAT ACCGAGGGCC CTTATTATGA TCCGAGTCTT CCGAATCTAT 
TTCCGATTTG AATTGCCACG TAGCCGGATA ACCAACATTC TCAAGCGCTC AGGGGAGCAG 
ATCTGGAGTG TGACCATATT TTTGCTATTC TTCCTTCTTC TTTATGGAAT CCTGGGGGTT 
CAGATGTTTG GCACATTCAA TCATCATTGC GTCACCAATG AAACAGAAAA AGAGAAAGTG 
ACATGGAACA GCCTAGCAAT CCCGGATACA CACTGCTCTC CGAACGGGGA GGGATATCAG 
TGTCCAGTGG GCTTTAAGTG TGTGGATCTG GAAGATTATG GACTCAGTAG ACAAGAACTT 
GGATACAGTG GATTCAATGA GCTTGGCACC AGTATATTCA CTGTATATGA GGCTGCGTCT 
CAGGAGGGCT GGGTCTTCAT CATGTATCGA GCCATCGACA GCTTCCCACG CTGGCGCTCA 
TATTTCTACT TCCTCACGCT CATCTTCTTC CTCGCCTGGC TGGTCAAGAA TGTGTTCATT 
GCTGTGATTA TCGAAACGTT TGCTGAGATC AGAGTGCAGT TCCAGCAGAT GTGGGGATCC 
CGGAGCAGCA CCACGTCAAC AGCTACTACA CAGATGTTTC ACGAGGACGC GTCTGGAGGA 
TGGCAGCTCG TGGCGGTGGA CGTGAATAAA CCACATGGCC GGGCGCCTGC TTGCCTCCAG 
CAACTGATGC GATCTTCAGT TTTCCACATG TTCATCCTCA GTATGGTGGC TGTTGATGTG 
ATTGTAGCTG CGAGCAATTA CCACAGAGGT GACCATAAAC TCACCAATGA TGAGTTCTAC 
CTGGCTGAGG TCGCCTTCAC TGTGCTGTTT GATTTGGAGG CTCTGCTGAA AATCTGGTGT 
TTGGGCTTCA CTGGATACAT CAGTTCATCG CTGCACAAGT TTGAGTCGCT GCTTGTAGTG 
GGCACCACCT TGCACATCTA CCCTGACCTT TACCACTCGC AGTTCACCTA CTTTCAGGTT 
TTGCGAGTGG TCCGTCTAAT AAAAATCTCC CCTGCACTAG AGGACTTTGT GTACAAGATC 
TTTGGTCCAG GGAAGAAACT GGGCAGTCTA GTGGTGTTTA CTGCCAGTCT GCTCATAGTG 
ATGTCTGCCA TCAGTCTGCA GATGTTCTGC TTTGTGGAGG ATTTGGATCG TTTCACCACA 
TTTCCACGGG CGTTTATGTC CATGTTCCAG ATTCTCACGC AGGAGGGCTG GGTGGATGTC 
ATGGACCAAA CACTGGTGGC TGTGGGACAG ATGTGGGCTC CGGTGGTGGC CATATACTTT 
ATTCTGTATC ACCTTTTTGC CACACTGATC CTTCTGAGTC TTTTTGTTGC TGTTATTTTG 
GACAATCTTG AGTTGGATGA AGACCTGAAA AAGTTAAAAC AGCTGAAGCA GAGTGAAGCC 
AATGCTGATA CAAAAGAGAA ACTTCCTTTG CGACTGCGAA TCTTTGAGAA GTTCCCCAAT 
CGACCGCAGA TGGTGAAAAT CTCCAAGCTG CCTTCAGACT TCACTGTGCC CAGAATCAGG 
GAGAGTTTCA TGAAGCAGTT CATCGACCGA CAGCAGCAGG ACCCTAGCTG TCTGTTCCGT 
CGCCTTCCCT CAGCATCATC CTCGTCTTGC GACCATTCCA AACGCTCTGC CATTGAGGAT 
AATAAATACA TAGATCAGAA GCTACGAAAG TCAGTATTTA GCATCCGGGC TCGCAACCTA 
ATGGAAAAGG AAATCACTGT CAACAAAATT TTGCGAGCCT GCACCAGGCA GCGCATGCTG 
AGTGGCTCAT TTGAGGGTCA GCCAACTAAA GAGAGATCCA TTCTCAGTGT CCAGCATCAC 
ATCCGCCAAG AACGCCGGTC CCTCAGACAT GGCTCTACCA GCCAGAGAAT CAGCAGAGGG 
AAATCCTTGG AAACACTCAC TCAGGACCAC TCCAGCACGG TCCGCTACCG TAATGCCCAG 
CGGGAGGACA GTGAAATCAA GATGATACAA GAGAAGAAGG AGCAGGCTGA GATGAAACGA 
AAAGTCCAAG AGGAGGAACT TCGGGAAAAC CATCCATACT TTGACAAGCC ACTCTTCATC 
GTTGGTAGAG AGCATCGATT CAGAAACTTT TGCCGAATGA TTGTTAGAGC CCGTTTTAAT 
GCGTCAAATA CGGATCCCAA TACAGGCGCA GTGAACAGCA CAAAATACCA TCAATTATAT 
GATTTGCTGG GCTTGGTAAC CTATCTGGAC TGGGTGATGA TCGTGGTCAC TATCTGCTCT 
TGTATTTCCA TGATGTTTGA GTCTCCTTTT ACTAGAGTCA TGCATGTCCC AACCCTGCAG 
ATTGGAGAAT ATGTGTTCGT CATCTTTATG AGCATTGAGC TGAACCTGAA GATTATGGCA 
GATGGCCTGT TCTTCACTCC CACTGCAGTC ATCCGAGACT TTGGCGGCGT CATGGACATC 
TTCATCTACT TAGTAAGTTT GATCTTTTTA TGCTGGCTGC CAAATAATGT GCCCCCTGAG 
TCTGGTGCCC AGCTCCTCAT GATGTTACGT TGCTTCCGGC CACTGAGAAT CTTTAAACTG 
GTGCCCCAAA TGAGGAAGGT GGTTCGAGAG GTCCTCAAGG GTTTCAAAGA GATTTTTCTG 
GTCTCTATTC TTCTCCTGAC ACTAATGCTG GTGTTCGCAA CCTTCGGGGT GCAACTCTTT 
GCTGGAAAAC TTGCCAAATG TAATGATCCT CATATTTCTA GCAAGGATGA CTGTCACGGC 
ATCTTTAGAA TCAATGTGAG CATTTCAAAG AACCTGAATT TGAAGCTCAA GGATGGGGAG 
AAGAAACCAG GATTTTGGGT GCCAAGAGTT TGGGCAAACC CTCGAAACTT CAATTTTGAT 
AATGTGGGCA ATGCCATGCT GGCTCTGTTT GAAGTGTTGT CGCTGAAGGG TTGGGTGGAG 
GTGAGAGATG TCATCATTCA CAGAGTAGGA CCGATTCATG GTATCTACAT CCATGTCTTT 
GTGTTCTTGG GATGTATGAT CGGACTCACC CTTTTTGTTG GCGTTGTCAT TGCCAACTTT 
AATGAAAATA AGGGCACTGC TCTGTTGACT GTGGATCAGA GGAGATGGGA AGATTTAAAG 
AGTCGACTCA AGATTGCTCA GCCCCTCCAC CTCCCTCCAC GTCCAGAAAA TGGTGGATTT 
CGTGCAAAGA TGTATGACAT CACCCAACAT CCCTTTTTCA AGCGAGGGAT TGCTGTGCTG 
GTTTTAGCAC AGTCTGTCCT CCTGTCGGTT AAGTGGGATG AGCTTGGCGC AGTTACGTTT 
CCCTTGGCCA CAATGTCAGT GGTTTTCACT TTCATTTTTG TTTTGGAGGT GACTATGAAG 
CTGATAGCCA TGTCACCAGC TGGATACTGG CAGAGCAGGA GGAACAGATA TGACCTACTC 
GTCACATCCC TCGGGCTCAT ATGGATCATT CTGCACTTCG CTTTACAGAA TGCTTACACA 
TACATGATGG GCACCTGTGT GATAGTATTC AGATTTTTCA CCATATGCGG AAAGCATGTG 
ACGTTAAAGA TGCTTCTGCT TACTGTGGTC GTCAGTATGT ACAAGAGTTT CTTCATTATC 
GTGGGCATGT TTTTGCTGCT GCTGTGCTAC GCTTTCGCCG GCGTCGTTCT TTTTGGCACC 
GTCAAATACG GAGAGAACAT TAACAGACAC GCAAACTTCT CCACCGCAGG GAAAGCCATC 
ACCGTACTTA TTCGAATAGT CACAGGTGAA GACTGGAATA AGATCATGCA TGACTGCATG 
GTGCAGCCGC CGTTCTGCAC CCCAGACAAA CATCGCTACT GGGAGACGGA CTGCGGCAAC 
TACGCTGGAG CCCTCATTTA CTTCTGCTCC TTCTATGTCA TCATCGCTTA CATCATGCTC 
AACCTGCTCG TAGCCATCAT TGTGGAGAAT TTCTCTTTGT TCTACTCCAC CGAAGAGGAC 
CAGTTGCTAA GCTACAACGA CCTGCGTCAC TTCCAGATCA TCTGGAACAT GGTGGATGAC 
AAGCGGGAGG GAGTGATTCC GACCTCTCGG GTGAAGTTTC TGCTCCGTCT GCTGAGAGGT 
CGTCTGGAAG TGGATCTGGA CAAAGACAAG CTTCTCTTTA AACACATGTG TTATGAGATG 
GAGCGTCTGC ACAATGGAGG AGACGTCACC TTTCATGATG TACTGAGCAT GCTGTCGTAT 
CGTTCAGTTG ATATCCGCAA GAGTCTTCAG CTGGAGGAGC TGTTGGCAAG AGAGCAGCTT 
GAATACACTA TAGAGGAGGA AGTGGCCAAA CAAACCATCC GCATGTGGTT GAAGAAGTGC 
CTGAAACGCA TCAGAGCCAA ACAGCAGCAG TCATGCAGCA TCATCCTCAG TCTGAGAGAG 
AGTCAACAGC AAGACCTGCG GCGGCTGCTC AACCCCCCCA GCATCGAGAC CACCGTCCCC 
AGCGAAGACA CCAACACACA CAACCAGGAC AATCCCACCC AGCCTGAGCA GAACAGCGGC 
CTGCAGACGT TACTGAGCCC CACTCTGTCA GACCGCAGTG GATACAGACA GGACTCTGCT 
GACCGACCCC AGAGGAAGCT GGGACAGTGG AGACTTCCTG CAGGCCGCAC AAGCGTGAAG 
TCCATGGTGT GTAAGATGAA CCCGGTGACT GACGAGGCGT CCTCAGGCTC TGAAGTTAAG 
AAGTGGTGGA CGCGTCAACT GACTGTGGAA AGTGATGAAA GTGGAGACGA CCTCATTGAT 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303014.1
CDS	184..5409
Translation	MPGMNAPMRSLTIHSLYSTASPSMLKRKQSSRVEAQPMTDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIKGENSYVKDRWCMFDGFMVVFLWVSLVLQVFEIAELVDQMSPWGMLRIPRALIMIRVFRIYFRFELPRSRITNILKRSGEQIWSVTIFLLFFLLLYGILGVQMFGTFNHHCVTNETEKEKVTWNSLAIPDTHCSPNGEGYQCPVGFKCVDLEDYGLSRQELGYSGFNELGTSIFTVYEAASQEGWVFIMYRAIDSFPRWRSYFYFLTLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDASGGWQLVAVDVNKPHGRAPACLQQLMRSSVFHMFILSMVAVDVIVAASNYHRGDHKLTNDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFESLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEDLDRFTTFPRAFMSMFQILTQEGWVDVMDQTLVAVGQMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPRIRESFMKQFIDRQQQDPSCLFRRLPSASSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLMEKEITVNKILRACTRQRMLSGSFEGQPTKERSILSVQHHIRQERRSLRHGSTSQRISRGKSLETLTQDHSSTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRMIVRARFNASNTDPNTGAVNSTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFTRVMHVPTLQIGEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWLPNNVPPESGAQLLMMLRCFRPLRIFKLVPQMRKVVREVLKGFKEIFLVSILLLTLMLVFATFGVQLFAGKLAKCNDPHISSKDDCHGIFRINVSISKNLNLKLKDGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPENGGFRAKMYDITQHPFFKRGIAVLVLAQSVLLSVKWDELGAVTFPLATMSVVFTFIFVLEVTMKLIAMSPAGYWQSRRNRYDLLVTSLGLIWIILHFALQNAYTYMMGTCVIVFRFFTICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLIRIVTGEDWNKIMHDCMVQPPFCTPDKHRYWETDCGNYAGALIYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTSRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIILSLRESQQQDLRRLLNPPSIETTVPSEDTNTHNQDNPTQPEQNSGLQTLLSPTLSDRSGYRQDSADRPQRKLGQWRLPAGRTSVKSMVCKMNPVTDEASSGSEVKKWWTRQLTVESDESGDDLIDI

Target ORF information:

RefSeq Version	XM_009304739.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304739.2

ORF Insert Sequence:

ATGCCGGGGA TGAATGCACC AATGAGGAGC CTGACCATCC ACTCTCTCTA TAGCACAGCG 
AGCCCAAGCA TGCTGAAGAG GAAGCAGAGC TCGCGGGTCG AGGCCCAGCC TATGACTGAC 
TTTGGGCCAG ACGAAACACT GACAGACAGT GCTGACATTC TTTGGATCAA TAAACCATGG 
GTTCACTCTC TCCTGCGGGC CTGTGCCATA ATCAGTGTTA TTTCGGTTTG TATGAATACA 
CCTAAGACCT TTGAGCACTA CCCTCCCCTA CAGTATGTCA CCTTCGCCCT GGACACACTG 
CTGATGTTCC TGTACACCGC AGAGATGATC GCCAAAATGC ATATCAGGGG CATCATCAAG 
GGAGAAAACT CCTATGTTAA AGACCGCTGG TGCATGTTTG ATGGCTTCAT GGTGGTCTTC 
CTCTGGGTCT CTCTTGTGCT TCAGGTGTTT GAAATAGCGG AGCTCGTGGA CCAAATGTCA 
CCCTGGGGAA TGTTACGCAT ACCGAGGGCC CTTATTATGA TCCGAGTCTT CCGAATCTAT 
TTCCGATTTG AATTGCCACG TAGCCGGATA ACCAACATTC TCAAGCGCTC AGGGGAGCAG 
ATCTGGAGTG TGACCATATT TTTGCTATTC TTCCTTCTTC TTTATGGAAT CCTGGGGGTT 
CAGATGTTTG GCACATTCAA TCATCATTGC GTCACCAATG AAACAGAAAA AGAGAAAGTG 
ACATGGAACA GCCTAGCAAT CCCGGATACA CACTGCTCTC CGAACGGGGA GGGATATCAG 
TGTCCAGTGG GCTTTAAGTG TGTGGATCTG GAAGATTATG GACTCAGTAG ACAAGAACTT 
GGATACAGTG GATTCAATGA GCTTGGCACC AGTATATTCA CTGTATATGA GGCTGCGTCT 
CAGGAGGGCT GGGTCTTCAT CATGTATCGA GCCATCGACA GCTTCCCACG CTGGCGCTCA 
TATTTCTACT TCCTCACGCT CATCTTCTTC CTCGCCTGGC TGGTCAAGAA TGTGTTCATT 
GCTGTGATTA TCGAAACGTT TGCTGAGATC AGAGTGCAGT TCCAGCAGAT GTGGGGATCC 
CGGAGCAGCA CCACGTCAAC AGCTACTACA CAGATGTTTC ACGAGGACGC GTCTGGAGGA 
TGGCAGCTCG TGGCGGTGGA CGTGAATAAA CCACATGGCC GGGCGCCTGC TTGCCTCCAG 
CAACTGATGC GATCTTCAGT TTTCCACATG TTCATCCTCA GTATGGTGGC TGTTGATGTG 
ATTGTAGCTG CGAGCAATTA CCACAGAGGT GACCATAAAC TCACCAATGA TGAGTTCTAC 
CTGGCTGAGG TCGCCTTCAC TGTGCTGTTT GATTTGGAGG CTCTGCTGAA AATCTGGTGT 
TTGGGCTTCA CTGGATACAT CAGTTCATCG CTGCACAAGT TTGAGTCGCT GCTTGTAGTG 
GGCACCACCT TGCACATCTA CCCTGACCTT TACCACTCGC AGTTCACCTA CTTTCAGGTT 
TTGCGAGTGG TCCGTCTAAT AAAAATCTCC CCTGCACTAG AGGACTTTGT GTACAAGATC 
TTTGGTCCAG GGAAGAAACT GGGCAGTCTA GTGGTGTTTA CTGCCAGTCT GCTCATAGTG 
ATGTCTGCCA TCAGTCTGCA GATGTTCTGC TTTGTGGAGG ATTTGGATCG TTTCACCACA 
TTTCCACGGG CGTTTATGTC CATGTTCCAG ATTCTCACGC AGGAGGGCTG GGTGGATGTC 
ATGGACCAAA CACTGGTGGC TGTGGGACAG ATGTGGGCTC CGGTGGTGGC CATATACTTT 
ATTCTGTATC ACCTTTTTGC CACACTGATC CTTCTGAGTC TTTTTGTTGC TGTTATTTTG 
GACAATCTTG AGTTGGATGA AGACCTGAAA AAGTTAAAAC AGCTGAAGCA GAGTGAAGCC 
AATGCTGATA CAAAAGAGAA ACTTCCTTTG CGACTGCGAA TCTTTGAGAA GTTCCCCAAT 
CGACCGCAGA TGGTGAAAAT CTCCAAGCTG CCTTCAGACT TCACTGTGCC CAGAATCAGG 
GAGAGTTTCA TGAAGCAGTT CATCGACCGA CAGCAGCAGG ACCCTAGCTG TCTGTTCCGT 
CGCCTTCCCT CAGCATCATC CTCGTCTTGC GACCATTCCA AACGCTCTGC CATTGAGGAT 
AATAAATACA TAGATCAGAA GCTACGAAAG TCAGTATTTA GCATCCGGGC TCGCAACCTA 
ATGGAAAAGG AAATCACTGT CAACAAAATT TTGCGAGCCT GCACCAGGCA GCGCATGCTG 
AGTGGCTCAT TTGAGGGTCA GCCAACTAAA GAGAGATCCA TTCTCAGTGT CCAGCATCAC 
ATCCGCCAAG AACGCCGGTC CCTCAGACAT GGCTCTACCA GCCAGAGAAT CAGCAGAGGG 
AAATCCTTGG AAACACTCAC TCAGGACCAC TCCAGCACGG TCCGCTACCG TAATGCCCAG 
CGGGAGGACA GTGAAATCAA GATGATACAA GAGAAGAAGG AGCAGGCTGA GATGAAACGA 
AAAGTCCAAG AGGAGGAACT TCGGGAAAAC CATCCATACT TTGACAAGCC ACTCTTCATC 
GTTGGTAGAG AGCATCGATT CAGAAACTTT TGCCGAATGA TTGTTAGAGC CCGTTTTAAT 
GCGTCAAATA CGGATCCCAA TACAGGCGCA GTGAACAGCA CAAAATACCA TCAATTATAT 
GATTTGCTGG GCTTGGTAAC CTATCTGGAC TGGGTGATGA TCGTGGTCAC TATCTGCTCT 
TGTATTTCCA TGATGTTTGA GTCTCCTTTT ACTAGAGTCA TGCATGTCCC AACCCTGCAG 
ATTGGAGAAT ATGTGTTCGT CATCTTTATG AGCATTGAGC TGAACCTGAA GATTATGGCA 
GATGGCCTGT TCTTCACTCC CACTGCAGTC ATCCGAGACT TTGGCGGCGT CATGGACATC 
TTCATCTACT TAGTAAGTTT GATCTTTTTA TGCTGGCTGC CAAATAATGT GCCCCCTGAG 
TCTGGTGCCC AGCTCCTCAT GATGTTACGT TGCTTCCGGC CACTGAGAAT CTTTAAACTG 
GTGCCCCAAA TGAGGAAGGT GGTTCGAGAG GTCCTCAAGG GTTTCAAAGA GATTTTTCTG 
GTCTCTATTC TTCTCCTGAC ACTAATGCTG GTGTTCGCAA CCTTCGGGGT GCAACTCTTT 
GCTGGAAAAC TTGCCAAATG TAATGATCCT CATATTTCTA GCAAGGATGA CTGTCACGGC 
ATCTTTAGAA TCAATGTGAG CATTTCAAAG AACCTGAATT TGAAGCTCAA GGATGGGGAG 
AAGAAACCAG GATTTTGGGT GCCAAGAGTT TGGGCAAACC CTCGAAACTT CAATTTTGAT 
AATGTGGGCA ATGCCATGCT GGCTCTGTTT GAAGTGTTGT CGCTGAAGGG TTGGGTGGAG 
GTGAGAGATG TCATCATTCA CAGAGTAGGA CCGATTCATG GTATCTACAT CCATGTCTTT 
GTGTTCTTGG GATGTATGAT CGGACTCACC CTTTTTGTTG GCGTTGTCAT TGCCAACTTT 
AATGAAAATA AGGGCACTGC TCTGTTGACT GTGGATCAGA GGAGATGGGA AGATTTAAAG 
AGTCGACTCA AGATTGCTCA GCCCCTCCAC CTCCCTCCAC GTCCAGAAAA TGGTGGATTT 
CGTGCAAAGA TGTATGACAT CACCCAACAT CCCTTTTTCA AGCGAGGGAT TGCTGTGCTG 
GTTTTAGCAC AGTCTGTCCT CCTGTCGGTT AAGTGGGATG AGCTTGGCGC AGTTACGTTT 
CCCTTGGCCA CAATGTCAGT GGTTTTCACT TTCATTTTTG TTTTGGAGGT GACTATGAAG 
CTGATAGCCA TGTCACCAGC TGGATACTGG CAGAGCAGGA GGAACAGATA TGACCTACTC 
GTCACATCCC TCGGGCTCAT ATGGATCATT CTGCACTTCG CTTTACAGAA TGCTTACACA 
TACATGATGG GCACCTGTGT GATAGTATTC AGATTTTTCA CCATATGCGG AAAGCATGTG 
ACGTTAAAGA TGCTTCTGCT TACTGTGGTC GTCAGTATGT ACAAGAGTTT CTTCATTATC 
GTGGGCATGT TTTTGCTGCT GCTGTGCTAC GCTTTCGCCG GCGTCGTTCT TTTTGGCACC 
GTCAAATACG GAGAGAACAT TAACAGACAC GCAAACTTCT CCACCGCAGG GAAAGCCATC 
ACCGTACTTA TTCGAATAGT CACAGGTGAA GACTGGAATA AGATCATGCA TGACTGCATG 
GTGCAGCCGC CGTTCTGCAC CCCAGACAAA CATCGCTACT GGGAGACGGA CTGCGGCAAC 
TACGCTGGAG CCCTCATTTA CTTCTGCTCC TTCTATGTCA TCATCGCTTA CATCATGCTC 
AACCTGCTCG TAGCCATCAT TGTGGAGAAT TTCTCTTTGT TCTACTCCAC CGAAGAGGAC 
CAGTTGCTAA GCTACAACGA CCTGCGTCAC TTCCAGATCA TCTGGAACAT GGTGGATGAC 
AAGCGGGAGG GAGTGATTCC GACCTCTCGG GTGAAGTTTC TGCTCCGTCT GCTGAGAGGT 
CGTCTGGAAG TGGATCTGGA CAAAGACAAG CTTCTCTTTA AACACATGTG TTATGAGATG 
GAGCGTCTGC ACAATGGAGG AGACGTCACC TTTCATGATG TACTGAGCAT GCTGTCGTAT 
CGTTCAGTTG ATATCCGCAA GAGTCTTCAG CTGGAGGAGC TGTTGGCAAG AGAGCAGCTT 
GAATACACTA TAGAGGAGGA AGTGGCCAAA CAAACCATCC GCATGTGGTT GAAGAAGTGC 
CTGAAACGCA TCAGAGCCAA ACAGCAGCAG TCATGCAGCA TCATCCTCAG TCTGAGAGAG 
AGTCAACAGC AAGACCTGCG GCGGCTGCTC AACCCCCCCA GCATCGAGAC CACCGTCCCC 
AGCGAAGACA CCAACACACA CAACCAGGAC AATCCCACCC AGCCTGAGCA GAACAGCGGC 
CTGCAGACGT TACTGAGCCC CACTCTGTCA GACCGCAGTG GATACAGACA GGACTCTGCT 
GACCGACCCC AGAGGAAGCT GGGACAGTGG AGACTTCCTG CAGGCCGCAC AAGCGTGAAG 
TCCATGGTGT GTAAGATGAA CCCGGTGACT GACGAGGCGT CCTCAGGCTC TGAAGTTAAG 
AAGTGGTGGA CGCGTCAACT GACTGTGGAA AGTGATGAAA GTGGAGACGA CCTCATTGAT 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_009304740.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303015.1 Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X4, mRNA.

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CloneID	ODa41705
Clone ID Related Accession (Same CDS sequence)	XM_009304740.2 , XM_009304739.2 , XM_009304740.3
Accession Version	XM_009304740.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sodium leak channel non-selective protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGA TGAATGCACC AATGAGGAGC CTGACCATCC ACTCTCTCTA TAGCACAGCG 
AGCCCAAGCA TGCTGAAGAG GAAGCAGAGC TCGCGGGTCG AGGCCCAGCC TATGACTGAC 
TTTGGGCCAG ACGAAACACT GACAGACAGT GCTGACATTC TTTGGATCAA TAAACCATGG 
GTTCACTCTC TCCTGCGGGC CTGTGCCATA ATCAGTGTTA TTTCGGTTTG TATGAATACA 
CCTAAGACCT TTGAGCACTA CCCTCCCCTA CAGTATGTCA CCTTCGCCCT GGACACACTG 
CTGATGTTCC TGTACACCGC AGAGATGATC GCCAAAATGC ATATCAGGGG CATCATCAAG 
GGAGAAAACT CCTATGTTAA AGACCGCTGG TGCATGTTTG ATGGCTTCAT GGTGGTCTTC 
CTCTGGGTCT CTCTTGTGCT TCAGGTGTTT GAAATAGCGG AGCTCGTGGA CCAAATGTCA 
CCCTGGGGAA TGTTACGCAT ACCGAGGGCC CTTATTATGA TCCGAGTCTT CCGAATCTAT 
TTCCGATTTG AATTGCCACG TAGCCGGATA ACCAACATTC TCAAGCGCTC AGGGGAGCAG 
ATCTGGAGTG TGACCATATT TTTGCTATTC TTCCTTCTTC TTTATGGAAT CCTGGGGGTT 
CAGATGTTTG GCACATTCAA TCATCATTGC GTCACCAATG AAACAGAAAA AGAGAAAGTG 
ACATGGAACA GCCTAGCAAT CCCGGATACA CACTGCTCTC CGAACGGGGA GGGATATCAG 
TGTCCAGTGG GCTTTAAGTG TGTGGATCTG GAAGATTATG GACTCAGTAG ACAAGAACTT 
GGATACAGTG GATTCAATGA GCTTGGCACC AGTATATTCA CTGTATATGA GGCTGCGTCT 
CAGGAGGGCT GGGTCTTCAT CATGTATCGA GCCATCGACA GCTTCCCACG CTGGCGCTCA 
TATTTCTACT TCCTCACGCT CATCTTCTTC CTCGCCTGGC TGGTCAAGAA TGTGTTCATT 
GCTGTGATTA TCGAAACGTT TGCTGAGATC AGAGTGCAGT TCCAGCAGAT GTGGGGATCC 
CGGAGCAGCA CCACGTCAAC AGCTACTACA CAGATGTTTC ACGAGGACGC GTCTGGAGGA 
TGGCAGCTCG TGGCGGTGGA CGTGAATAAA CCACATGGCC GGGCGCCTGC TTGCCTCCAG 
CAACTGATGC GATCTTCAGT TTTCCACATG TTCATCCTCA GTATGGTGGC TGTTGATGTG 
ATTGTAGCTG CGAGCAATTA CCACAGAGGT GACCATAAAC TCACCAATGA TGAGTTCTAC 
CTGGCTGAGG TCGCCTTCAC TGTGCTGTTT GATTTGGAGG CTCTGCTGAA AATCTGGTGT 
TTGGGCTTCA CTGGATACAT CAGTTCATCG CTGCACAAGT TTGAGTCGCT GCTTGTAGTG 
GGCACCACCT TGCACATCTA CCCTGACCTT TACCACTCGC AGTTCACCTA CTTTCAGGTT 
TTGCGAGTGG TCCGTCTAAT AAAAATCTCC CCTGCACTAG AGGACTTTGT GTACAAGATC 
TTTGGTCCAG GGAAGAAACT GGGCAGTCTA GTGGTGTTTA CTGCCAGTCT GCTCATAGTG 
ATGTCTGCCA TCAGTCTGCA GATGTTCTGC TTTGTGGAGG ATTTGGATCG TTTCACCACA 
TTTCCACGGG CGTTTATGTC CATGTTCCAG ATTCTCACGC AGGAGGGCTG GGTGGATGTC 
ATGGACCAAA CACTGGTGGC TGTGGGACAG ATGTGGGCTC CGGTGGTGGC CATATACTTT 
ATTCTGTATC ACCTTTTTGC CACACTGATC CTTCTGAGTC TTTTTGTTGC TGTTATTTTG 
GACAATCTTG AGTTGGATGA AGACCTGAAA AAGTTAAAAC AGCTGAAGCA GAGTGAAGCC 
AATGCTGATA CAAAAGAGAA ACTTCCTTTG CGACTGCGAA TCTTTGAGAA GTTCCCCAAT 
CGACCGCAGA TGGTGAAAAT CTCCAAGCTG CCTTCAGACT TCACTGTGCC CAGAATCAGG 
GAGAGTTTCA TGAAGCAGTT CATCGACCGA CAGCAGCAGG ACCCTAGCTG TCTGTTCCGT 
CGCCTTCCCT CAGCATCATC CTCGTCTTGC GACCATTCCA AACGCTCTGC CATTGAGGAT 
AATAAATACA TAGATCAGAA GCTACGAAAG TCAGTATTTA GCATCCGGGC TCGCAACCTA 
ATGGAAAAGG AAATCACTGT CAACAAAATT TTGCGAGCCT GCACCAGGCA GCGCATGCTG 
AGTGGCTCAT TTGAGGGTCA GCCAACTAAA GAGAGATCCA TTCTCAGTGT CCAGCATCAC 
ATCCGCCAAG AACGCCGGTC CCTCAGACAT GGCTCTACCA GCCAGAGAAT CAGCAGAGGG 
AAATCCTTGG AAACACTCAC TCAGGACCAC TCCAGCACGG TCCGCTACCG TAATGCCCAG 
CGGGAGGACA GTGAAATCAA GATGATACAA GAGAAGAAGG AGCAGGCTGA GATGAAACGA 
AAAGTCCAAG AGGAGGAACT TCGGGAAAAC CATCCATACT TTGACAAGCC ACTCTTCATC 
GTTGGTAGAG AGCATCGATT CAGAAACTTT TGCCGAATGA TTGTTAGAGC CCGTTTTAAT 
GCGTCAAATA CGGATCCCAA TACAGGCGCA GTGAACAGCA CAAAATACCA TCAATTATAT 
GATTTGCTGG GCTTGGTAAC CTATCTGGAC TGGGTGATGA TCGTGGTCAC TATCTGCTCT 
TGTATTTCCA TGATGTTTGA GTCTCCTTTT ACTAGAGTCA TGCATGTCCC AACCCTGCAG 
ATTGGAGAAT ATGTGTTCGT CATCTTTATG AGCATTGAGC TGAACCTGAA GATTATGGCA 
GATGGCCTGT TCTTCACTCC CACTGCAGTC ATCCGAGACT TTGGCGGCGT CATGGACATC 
TTCATCTACT TAGTAAGTTT GATCTTTTTA TGCTGGCTGC CAAATAATGT GCCCCCTGAG 
TCTGGTGCCC AGCTCCTCAT GATGTTACGT TGCTTCCGGC CACTGAGAAT CTTTAAACTG 
GTGCCCCAAA TGAGGAAGGT GGTTCGAGAG GTCCTCAAGG GTTTCAAAGA GATTTTTCTG 
GTCTCTATTC TTCTCCTGAC ACTAATGCTG GTGTTCGCAA CCTTCGGGGT GCAACTCTTT 
GCTGGAAAAC TTGCCAAATG TAATGATCCT CATATTTCTA GCAAGGATGA CTGTCACGGC 
ATCTTTAGAA TCAATGTGAG CATTTCAAAG AACCTGAATT TGAAGCTCAA GGATGGGGAG 
AAGAAACCAG GATTTTGGGT GCCAAGAGTT TGGGCAAACC CTCGAAACTT CAATTTTGAT 
AATGTGGGCA ATGCCATGCT GGCTCTGTTT GAAGTGTTGT CGCTGAAGGG TTGGGTGGAG 
GTGAGAGATG TCATCATTCA CAGAGTAGGA CCGATTCATG GTATCTACAT CCATGTCTTT 
GTGTTCTTGG GATGTATGAT CGGACTCACC CTTTTTGTTG GCGTTGTCAT TGCCAACTTT 
AATGAAAATA AGGGCACTGC TCTGTTGACT GTGGATCAGA GGAGATGGGA AGATTTAAAG 
AGTCGACTCA AGATTGCTCA GCCCCTCCAC CTCCCTCCAC GTCCAGAAAA TGGTGGATTT 
CGTGCAAAGA TGTATGACAT CACCCAACAT CCCTTTTTCA AGCGAGGGAT TGCTGTGCTG 
GTTTTAGCAC AGTCTGTCCT CCTGTCGGTT AAGTGGGATG AGCTTGGCGC AGTTACGTTT 
CCCTTGGCCA CAATGTCAGT GGTTTTCACT TTCATTTTTG TTTTGGAGGT GACTATGAAG 
CTGATAGCCA TGTCACCAGC TGGATACTGG CAGAGCAGGA GGAACAGATA TGACCTACTC 
GTCACATCCC TCGGGCTCAT ATGGATCATT CTGCACTTCG CTTTACAGAA TGCTTACACA 
TACATGATGG GCACCTGTGT GATAGTATTC AGATTTTTCA CCATATGCGG AAAGCATGTG 
ACGTTAAAGA TGCTTCTGCT TACTGTGGTC GTCAGTATGT ACAAGAGTTT CTTCATTATC 
GTGGGCATGT TTTTGCTGCT GCTGTGCTAC GCTTTCGCCG GCGTCGTTCT TTTTGGCACC 
GTCAAATACG GAGAGAACAT TAACAGACAC GCAAACTTCT CCACCGCAGG GAAAGCCATC 
ACCGTACTTA TTCGAATAGT CACAGGTGAA GACTGGAATA AGATCATGCA TGACTGCATG 
GTGCAGCCGC CGTTCTGCAC CCCAGACAAA CATCGCTACT GGGAGACGGA CTGCGGCAAC 
TACGCTGGAG CCCTCATTTA CTTCTGCTCC TTCTATGTCA TCATCGCTTA CATCATGCTC 
AACCTGCTCG TAGCCATCAT TGTGGAGAAT TTCTCTTTGT TCTACTCCAC CGAAGAGGAC 
CAGTTGCTAA GCTACAACGA CCTGCGTCAC TTCCAGATCA TCTGGAACAT GGTGGATGAC 
AAGCGGGAGG GAGTGATTCC GACCTCTCGG GTGAAGTTTC TGCTCCGTCT GCTGAGAGGT 
CGTCTGGAAG TGGATCTGGA CAAAGACAAG CTTCTCTTTA AACACATGTG TTATGAGATG 
GAGCGTCTGC ACAATGGAGG AGACGTCACC TTTCATGATG TACTGAGCAT GCTGTCGTAT 
CGTTCAGTTG ATATCCGCAA GAGTCTTCAG CTGGAGGAGC TGTTGGCAAG AGAGCAGCTT 
GAATACACTA TAGAGGAGGA AGTGGCCAAA CAAACCATCC GCATGTGGTT GAAGAAGTGC 
CTGAAACGCA TCAGAGCCAA ACAGCAGCAG TCATGCAGCA TCATCCTCAG TCTGAGAGAG 
AGTCAACAGC AAGACCTGCG GCGGCTGCTC AACCCCCCCA GCATCGAGAC CACCGTCCCC 
AGCGAAGACA CCAACACACA CAACCAGGAC AATCCCACCC AGCCTGAGCA GAACAGCGGC 
CTGCAGACGT TACTGAGCCC CACTCTGTCA GACCGCAGTG GATACAGACA GGACTCTGCT 
GACCGACCCC AGAGGAAGCT GGGACAGTGG AGACTTCCTG CAGGCCGCAC AAGCGTGAAG 
TCCATGGTGT GTAAGATGAA CCCGGTGACT GACGAGGCGT CCTCAGGCTC TGAAGTTAAG 
AAGTGGTGGA CGCGTCAACT GACTGTGGAA AGTGATGAAA GTGGAGACGA CCTCATTGAT 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303015.1
CDS	178..5403
Translation	MPGMNAPMRSLTIHSLYSTASPSMLKRKQSSRVEAQPMTDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIKGENSYVKDRWCMFDGFMVVFLWVSLVLQVFEIAELVDQMSPWGMLRIPRALIMIRVFRIYFRFELPRSRITNILKRSGEQIWSVTIFLLFFLLLYGILGVQMFGTFNHHCVTNETEKEKVTWNSLAIPDTHCSPNGEGYQCPVGFKCVDLEDYGLSRQELGYSGFNELGTSIFTVYEAASQEGWVFIMYRAIDSFPRWRSYFYFLTLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDASGGWQLVAVDVNKPHGRAPACLQQLMRSSVFHMFILSMVAVDVIVAASNYHRGDHKLTNDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFESLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEDLDRFTTFPRAFMSMFQILTQEGWVDVMDQTLVAVGQMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPRIRESFMKQFIDRQQQDPSCLFRRLPSASSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLMEKEITVNKILRACTRQRMLSGSFEGQPTKERSILSVQHHIRQERRSLRHGSTSQRISRGKSLETLTQDHSSTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRMIVRARFNASNTDPNTGAVNSTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFTRVMHVPTLQIGEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWLPNNVPPESGAQLLMMLRCFRPLRIFKLVPQMRKVVREVLKGFKEIFLVSILLLTLMLVFATFGVQLFAGKLAKCNDPHISSKDDCHGIFRINVSISKNLNLKLKDGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPENGGFRAKMYDITQHPFFKRGIAVLVLAQSVLLSVKWDELGAVTFPLATMSVVFTFIFVLEVTMKLIAMSPAGYWQSRRNRYDLLVTSLGLIWIILHFALQNAYTYMMGTCVIVFRFFTICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLIRIVTGEDWNKIMHDCMVQPPFCTPDKHRYWETDCGNYAGALIYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTSRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIILSLRESQQQDLRRLLNPPSIETTVPSEDTNTHNQDNPTQPEQNSGLQTLLSPTLSDRSGYRQDSADRPQRKLGQWRLPAGRTSVKSMVCKMNPVTDEASSGSEVKKWWTRQLTVESDESGDDLIDI

Target ORF information:

RefSeq Version	XM_009304740.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sodium leak channel, non-selective (nalcn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304740.3

ORF Insert Sequence:

ATGCCGGGGA TGAATGCACC AATGAGGAGC CTGACCATCC ACTCTCTCTA TAGCACAGCG 
AGCCCAAGCA TGCTGAAGAG GAAGCAGAGC TCGCGGGTCG AGGCCCAGCC TATGACTGAC 
TTTGGGCCAG ACGAAACACT GACAGACAGT GCTGACATTC TTTGGATCAA TAAACCATGG 
GTTCACTCTC TCCTGCGGGC CTGTGCCATA ATCAGTGTTA TTTCGGTTTG TATGAATACA 
CCTAAGACCT TTGAGCACTA CCCTCCCCTA CAGTATGTCA CCTTCGCCCT GGACACACTG 
CTGATGTTCC TGTACACCGC AGAGATGATC GCCAAAATGC ATATCAGGGG CATCATCAAG 
GGAGAAAACT CCTATGTTAA AGACCGCTGG TGCATGTTTG ATGGCTTCAT GGTGGTCTTC 
CTCTGGGTCT CTCTTGTGCT TCAGGTGTTT GAAATAGCGG AGCTCGTGGA CCAAATGTCA 
CCCTGGGGAA TGTTACGCAT ACCGAGGGCC CTTATTATGA TCCGAGTCTT CCGAATCTAT 
TTCCGATTTG AATTGCCACG TAGCCGGATA ACCAACATTC TCAAGCGCTC AGGGGAGCAG 
ATCTGGAGTG TGACCATATT TTTGCTATTC TTCCTTCTTC TTTATGGAAT CCTGGGGGTT 
CAGATGTTTG GCACATTCAA TCATCATTGC GTCACCAATG AAACAGAAAA AGAGAAAGTG 
ACATGGAACA GCCTAGCAAT CCCGGATACA CACTGCTCTC CGAACGGGGA GGGATATCAG 
TGTCCAGTGG GCTTTAAGTG TGTGGATCTG GAAGATTATG GACTCAGTAG ACAAGAACTT 
GGATACAGTG GATTCAATGA GCTTGGCACC AGTATATTCA CTGTATATGA GGCTGCGTCT 
CAGGAGGGCT GGGTCTTCAT CATGTATCGA GCCATCGACA GCTTCCCACG CTGGCGCTCA 
TATTTCTACT TCCTCACGCT CATCTTCTTC CTCGCCTGGC TGGTCAAGAA TGTGTTCATT 
GCTGTGATTA TCGAAACGTT TGCTGAGATC AGAGTGCAGT TCCAGCAGAT GTGGGGATCC 
CGGAGCAGCA CCACGTCAAC AGCTACTACA CAGATGTTTC ACGAGGACGC GTCTGGAGGA 
TGGCAGCTCG TGGCGGTGGA CGTGAATAAA CCACATGGCC GGGCGCCTGC TTGCCTCCAG 
CAACTGATGC GATCTTCAGT TTTCCACATG TTCATCCTCA GTATGGTGGC TGTTGATGTG 
ATTGTAGCTG CGAGCAATTA CCACAGAGGT GACCATAAAC TCACCAATGA TGAGTTCTAC 
CTGGCTGAGG TCGCCTTCAC TGTGCTGTTT GATTTGGAGG CTCTGCTGAA AATCTGGTGT 
TTGGGCTTCA CTGGATACAT CAGTTCATCG CTGCACAAGT TTGAGTCGCT GCTTGTAGTG 
GGCACCACCT TGCACATCTA CCCTGACCTT TACCACTCGC AGTTCACCTA CTTTCAGGTT 
TTGCGAGTGG TCCGTCTAAT AAAAATCTCC CCTGCACTAG AGGACTTTGT GTACAAGATC 
TTTGGTCCAG GGAAGAAACT GGGCAGTCTA GTGGTGTTTA CTGCCAGTCT GCTCATAGTG 
ATGTCTGCCA TCAGTCTGCA GATGTTCTGC TTTGTGGAGG ATTTGGATCG TTTCACCACA 
TTTCCACGGG CGTTTATGTC CATGTTCCAG ATTCTCACGC AGGAGGGCTG GGTGGATGTC 
ATGGACCAAA CACTGGTGGC TGTGGGACAG ATGTGGGCTC CGGTGGTGGC CATATACTTT 
ATTCTGTATC ACCTTTTTGC CACACTGATC CTTCTGAGTC TTTTTGTTGC TGTTATTTTG 
GACAATCTTG AGTTGGATGA AGACCTGAAA AAGTTAAAAC AGCTGAAGCA GAGTGAAGCC 
AATGCTGATA CAAAAGAGAA ACTTCCTTTG CGACTGCGAA TCTTTGAGAA GTTCCCCAAT 
CGACCGCAGA TGGTGAAAAT CTCCAAGCTG CCTTCAGACT TCACTGTGCC CAGAATCAGG 
GAGAGTTTCA TGAAGCAGTT CATCGACCGA CAGCAGCAGG ACCCTAGCTG TCTGTTCCGT 
CGCCTTCCCT CAGCATCATC CTCGTCTTGC GACCATTCCA AACGCTCTGC CATTGAGGAT 
AATAAATACA TAGATCAGAA GCTACGAAAG TCAGTATTTA GCATCCGGGC TCGCAACCTA 
ATGGAAAAGG AAATCACTGT CAACAAAATT TTGCGAGCCT GCACCAGGCA GCGCATGCTG 
AGTGGCTCAT TTGAGGGTCA GCCAACTAAA GAGAGATCCA TTCTCAGTGT CCAGCATCAC 
ATCCGCCAAG AACGCCGGTC CCTCAGACAT GGCTCTACCA GCCAGAGAAT CAGCAGAGGG 
AAATCCTTGG AAACACTCAC TCAGGACCAC TCCAGCACGG TCCGCTACCG TAATGCCCAG 
CGGGAGGACA GTGAAATCAA GATGATACAA GAGAAGAAGG AGCAGGCTGA GATGAAACGA 
AAAGTCCAAG AGGAGGAACT TCGGGAAAAC CATCCATACT TTGACAAGCC ACTCTTCATC 
GTTGGTAGAG AGCATCGATT CAGAAACTTT TGCCGAATGA TTGTTAGAGC CCGTTTTAAT 
GCGTCAAATA CGGATCCCAA TACAGGCGCA GTGAACAGCA CAAAATACCA TCAATTATAT 
GATTTGCTGG GCTTGGTAAC CTATCTGGAC TGGGTGATGA TCGTGGTCAC TATCTGCTCT 
TGTATTTCCA TGATGTTTGA GTCTCCTTTT ACTAGAGTCA TGCATGTCCC AACCCTGCAG 
ATTGGAGAAT ATGTGTTCGT CATCTTTATG AGCATTGAGC TGAACCTGAA GATTATGGCA 
GATGGCCTGT TCTTCACTCC CACTGCAGTC ATCCGAGACT TTGGCGGCGT CATGGACATC 
TTCATCTACT TAGTAAGTTT GATCTTTTTA TGCTGGCTGC CAAATAATGT GCCCCCTGAG 
TCTGGTGCCC AGCTCCTCAT GATGTTACGT TGCTTCCGGC CACTGAGAAT CTTTAAACTG 
GTGCCCCAAA TGAGGAAGGT GGTTCGAGAG GTCCTCAAGG GTTTCAAAGA GATTTTTCTG 
GTCTCTATTC TTCTCCTGAC ACTAATGCTG GTGTTCGCAA CCTTCGGGGT GCAACTCTTT 
GCTGGAAAAC TTGCCAAATG TAATGATCCT CATATTTCTA GCAAGGATGA CTGTCACGGC 
ATCTTTAGAA TCAATGTGAG CATTTCAAAG AACCTGAATT TGAAGCTCAA GGATGGGGAG 
AAGAAACCAG GATTTTGGGT GCCAAGAGTT TGGGCAAACC CTCGAAACTT CAATTTTGAT 
AATGTGGGCA ATGCCATGCT GGCTCTGTTT GAAGTGTTGT CGCTGAAGGG TTGGGTGGAG 
GTGAGAGATG TCATCATTCA CAGAGTAGGA CCGATTCATG GTATCTACAT CCATGTCTTT 
GTGTTCTTGG GATGTATGAT CGGACTCACC CTTTTTGTTG GCGTTGTCAT TGCCAACTTT 
AATGAAAATA AGGGCACTGC TCTGTTGACT GTGGATCAGA GGAGATGGGA AGATTTAAAG 
AGTCGACTCA AGATTGCTCA GCCCCTCCAC CTCCCTCCAC GTCCAGAAAA TGGTGGATTT 
CGTGCAAAGA TGTATGACAT CACCCAACAT CCCTTTTTCA AGCGAGGGAT TGCTGTGCTG 
GTTTTAGCAC AGTCTGTCCT CCTGTCGGTT AAGTGGGATG AGCTTGGCGC AGTTACGTTT 
CCCTTGGCCA CAATGTCAGT GGTTTTCACT TTCATTTTTG TTTTGGAGGT GACTATGAAG 
CTGATAGCCA TGTCACCAGC TGGATACTGG CAGAGCAGGA GGAACAGATA TGACCTACTC 
GTCACATCCC TCGGGCTCAT ATGGATCATT CTGCACTTCG CTTTACAGAA TGCTTACACA 
TACATGATGG GCACCTGTGT GATAGTATTC AGATTTTTCA CCATATGCGG AAAGCATGTG 
ACGTTAAAGA TGCTTCTGCT TACTGTGGTC GTCAGTATGT ACAAGAGTTT CTTCATTATC 
GTGGGCATGT TTTTGCTGCT GCTGTGCTAC GCTTTCGCCG GCGTCGTTCT TTTTGGCACC 
GTCAAATACG GAGAGAACAT TAACAGACAC GCAAACTTCT CCACCGCAGG GAAAGCCATC 
ACCGTACTTA TTCGAATAGT CACAGGTGAA GACTGGAATA AGATCATGCA TGACTGCATG 
GTGCAGCCGC CGTTCTGCAC CCCAGACAAA CATCGCTACT GGGAGACGGA CTGCGGCAAC 
TACGCTGGAG CCCTCATTTA CTTCTGCTCC TTCTATGTCA TCATCGCTTA CATCATGCTC 
AACCTGCTCG TAGCCATCAT TGTGGAGAAT TTCTCTTTGT TCTACTCCAC CGAAGAGGAC 
CAGTTGCTAA GCTACAACGA CCTGCGTCAC TTCCAGATCA TCTGGAACAT GGTGGATGAC 
AAGCGGGAGG GAGTGATTCC GACCTCTCGG GTGAAGTTTC TGCTCCGTCT GCTGAGAGGT 
CGTCTGGAAG TGGATCTGGA CAAAGACAAG CTTCTCTTTA AACACATGTG TTATGAGATG 
GAGCGTCTGC ACAATGGAGG AGACGTCACC TTTCATGATG TACTGAGCAT GCTGTCGTAT 
CGTTCAGTTG ATATCCGCAA GAGTCTTCAG CTGGAGGAGC TGTTGGCAAG AGAGCAGCTT 
GAATACACTA TAGAGGAGGA AGTGGCCAAA CAAACCATCC GCATGTGGTT GAAGAAGTGC 
CTGAAACGCA TCAGAGCCAA ACAGCAGCAG TCATGCAGCA TCATCCTCAG TCTGAGAGAG 
AGTCAACAGC AAGACCTGCG GCGGCTGCTC AACCCCCCCA GCATCGAGAC CACCGTCCCC 
AGCGAAGACA CCAACACACA CAACCAGGAC AATCCCACCC AGCCTGAGCA GAACAGCGGC 
CTGCAGACGT TACTGAGCCC CACTCTGTCA GACCGCAGTG GATACAGACA GGACTCTGCT 
GACCGACCCC AGAGGAAGCT GGGACAGTGG AGACTTCCTG CAGGCCGCAC AAGCGTGAAG 
TCCATGGTGT GTAAGATGAA CCCGGTGACT GACGAGGCGT CCTCAGGCTC TGAAGTTAAG 
AAGTGGTGGA CGCGTCAACT GACTGTGGAA AGTGATGAAA GTGGAGACGA CCTCATTGAT 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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nalcn cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

FAX

オンラインフォーム