vps33b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	vps33b
Entrez Gene ID	541534
Full Name	vacuolar protein sorting 33B
Synonyms	zgc:110803
General protein information	Preferred Names vacuolar protein sorting 33B Names vacuolar protein sorting-associated protein 33B vacuolar sorting protein 33b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014348.1	NP_001014370.1	vacuolar protein sorting-associated protein 33B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	VPS33B	XP_510601.2
Mus musculus (house mouse)	Vps33b	NP_835171.2
Caenorhabditis elegans (roundworm)	vps-33.2	NP_495342.2
Canis lupus familiaris (dog)	VPS33B	XP_536197.2
Danio rerio (zebrafish)	vps33b	NP_001014370.1
	VPS33B	XP_001097946.1
Gallus gallus (chicken)	VPS33B	NP_001026519.1
Bos taurus (cattle)	VPS33B	NP_001039749.1
Homo sapiens (human)	VPS33B	NP_061138.3
Rattus norvegicus (Norway rat)	Vps33b	NP_071622.1
Xenopus tropicalis (tropical clawed frog)	vps33b	NP_001096504.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish models of human liver development and disease.
Wilkins BJ, Pack M
Comprehensive Physiology3(3)1213-30(2013 Jul)

Mutations in vacuolar H+ -ATPase subunits lead to biliary developmental defects in zebrafish.
EauClaire SF, Cui S, Ma L, Matous J, Marlow FL, Gupta T, Burgess HA, Abrams EW, Kapp LD, Granato M, Mullins MC, Matthews RP
Developmental biology365(2)434-44(2012 May)

Zebrafish vps33b, an ortholog of the gene responsible for human arthrogryposis-renal dysfunction-cholestasis syndrome, regulates biliary development downstream of the onecut transcription factor hnf6.
Matthews RP, Plumb-Rudewiez N, Lorent K, Gissen P, Johnson CA, Lemaigre F, Pack M
Development (Cambridge, England)132(23)5295-306(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

knockdown of atp6ap2 and vps33b had an additive negative effect on biliary development.
Title: Mutations in vacuolar H+ -ATPase subunits lead to biliary developmental defects in zebrafish.

findings identify vps33b is a novel downstream target gene of the hnf6/vhnf1 pathway that regulates bile duct development in zebrafish
Title: Zebrafish vps33b, an ortholog of the gene responsible for human arthrogryposis-renal dysfunction-cholestasis syndrome, regulates biliary development downstream of the onecut transcription factor hnf6.

The following vps33b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps33b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38441	NM_001014348.1 Latest version!	Danio rerio VPS33B late endosome and lysosome associated (vps33b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps33b ( NM_001014348.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001014370.1 Danio rerio VPS33B late endosome and lysosome associated (vps33b), mRNA.

Price & Availability↑

CloneID	ODa38441
Clone ID Related Accession (Same CDS sequence)	NM_001014348.1
Accession Version	NM_001014348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 33B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091824.1. ##Evidence-Data-START## Transcript exon combination :: BC091824.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCAGA CAGAGAGGAG AGATGCTCCT GAACTGCCCG ACTTCTCATT GCTCAAAAGA 
CTGGCAAGAG ACCAGCTCAT CTTTCTGCTG GAACAGTTGC CAGGGAAGAA GGATTTATTT 
ATTGATGCAG ATTTGATGAG TCCTCTAGAT CGAATTGCAA ATGTCACAAT CCTAAAGCAA 
CATGAAGTCG ACAAACTATA CAAAGTTGAG CTTAAACCAA TCGTCAGCAG TTCAGACCAG 
CTGTGCTTCC TCATACGTCC TAGAATACAA ACTGTGAAGT GGATTTCAGA TTTAGTTAAT 
TCAGACAAGG TAGCTGGACG ATTCCGAAGA TATAAGATCA TATTTACCCC ACAAAAGTTT 
TATGCCTGTG AAACAGTTTT GGAGGAACAG GGTGTCTATG GAGATGTTAC AACCGATGAA 
TGGAATTTCT ACATTCTTCC TCTGGATGAC GATATCCTCA GCTTAGAGCT GCCAGAGTTC 
TTCAGGGATA ACTTTCTGGA GGGAGACCAG CGCTGGGTGA CCACTGGAGG GGGTGCTTTA 
CATCTTTTAC AGTCTGTCTA TGGTTCCTTC TCAAAGGTTT ATGGGATTGG AAGATGTGCC 
AAGATGGTGT ATGAATCCTG GAGGGAACTA ATGGAGGAGG GTGAACAAAG AACAAGACAA 
CCTGAATTTG CCAAAGTATT TCTTATTGAC AGAGATGTGG ATTTTGTTAC TCCTCTCTGC 
TCTCAAGTGG TTTACGAGGG ATTGGTGGAT GACATTTTCA GAATAAAATG TAGCAGTGTG 
GAGTTTGGCC CTGATGTCAC TTCCTCTGAC AAGAGTATCA AAGTGATGCT TAACTCTCAA 
GATAAAGTGT TCAATGAAAT AAGGAATGAA CATTTTTCCA ATGTGTTTGG CTTTTTAAGT 
CAGAAGGCTA AAAATCTTCA GACAGCATAT GATAAACGGC GTGGGATGGA CATTCAGCAG 
ATGAAGGCGT TTGTTGCAGA TGAACTGAAA GGACTCAAAC AGGAACATCG TCTTCTTAGT 
CTTCATATTG GTGCAAGCGA ATCCATAATG AAGAAGAAAA CAAAGCAAGA TTTCCAGGAA 
TTGTTGAAAA CTGAACATTC ATTACTTGAA GGCTTTGAGA TCCGGGAATG TATTGCTTAC 
ATTGAAGAAC ATATCAACAG ACAGGTGTCG ATGATTGATA GCCTTAGACT CCTTTGTCTG 
TTGTCTATCA CAGAAAATGG CCTTCTGTCC AAAGACTATC GATCTCTTAA AGCTCAGTAC 
CTTCAGAGTT ATGGTATAGA GCACCTGCTG ACGTTTGCAA ACCTGAGGCA GCTGGGACTG 
TTGGAAGAAC AGCAAACTGG AGAAACACTG ACAGTCATGG AGAGTAAAGT AGGGAAACTT 
GTCAATGACA AGACTGCAGG GAAGCTGACG GATGCCTTCT CCTCACTGGC TAAGAAGAGC 
AATTTCCGAG CTCTGAGTAA AAGACTGGCT CTGGTGCCAA AGTCTGGTGA GGAATATGAC 
CTTCGCGTTC CACGAGACAT GGCTTATATT TTCAGCGGTG CTTACATTCC CCTGAGCTGC 
AAACTGATTG AACAGGTGCT GGAGAGAGAT GGGTGGACAG GTCTGGAGGA GGTCACACGC 
ATGCTGAATG GACAGGACTT TGCTGTAACA GGTGGCAGCA GTAGTTCAGA AGCCAGAAAC 
AAAAGTAATG GTCAAAGGAT CATTCTAGTG ATGTTCCTTG GTGGCTGCAC TTACTCTGAG 
ATCTCCGCAC TCCGATTCCT TGGCAAGGAA AGAGGTTGCA GATTCATAGT GCTCACAACA 
GCCATCACCA ACAGTGGACG GCTTCTGGAA GCTTTACTGG ATAAACATGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014370.1
CDS	40..1893
Translation	MAQTERRDAPELPDFSLLKRLARDQLIFLLEQLPGKKDLFIDADLMSPLDRIANVTILKQHEVDKLYKVELKPIVSSSDQLCFLIRPRIQTVKWISDLVNSDKVAGRFRRYKIIFTPQKFYACETVLEEQGVYGDVTTDEWNFYILPLDDDILSLELPEFFRDNFLEGDQRWVTTGGGALHLLQSVYGSFSKVYGIGRCAKMVYESWRELMEEGEQRTRQPEFAKVFLIDRDVDFVTPLCSQVVYEGLVDDIFRIKCSSVEFGPDVTSSDKSIKVMLNSQDKVFNEIRNEHFSNVFGFLSQKAKNLQTAYDKRRGMDIQQMKAFVADELKGLKQEHRLLSLHIGASESIMKKKTKQDFQELLKTEHSLLEGFEIRECIAYIEEHINRQVSMIDSLRLLCLLSITENGLLSKDYRSLKAQYLQSYGIEHLLTFANLRQLGLLEEQQTGETLTVMESKVGKLVNDKTAGKLTDAFSSLAKKSNFRALSKRLALVPKSGEEYDLRVPRDMAYIFSGAYIPLSCKLIEQVLERDGWTGLEEVTRMLNGQDFAVTGGSSSSEARNKSNGQRIILVMFLGGCTYSEISALRFLGKERGCRFIVLTTAITNSGRLLEALLDKHV

Target ORF information:

RefSeq Version	NM_001014348.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio VPS33B late endosome and lysosome associated (vps33b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014348.1

ORF Insert Sequence:

ATGGCTCAGA CAGAGAGGAG AGATGCTCCT GAACTGCCCG ACTTCTCATT GCTCAAAAGA 
CTGGCAAGAG ACCAGCTCAT CTTTCTGCTG GAACAGTTGC CAGGGAAGAA GGATTTATTT 
ATTGATGCAG ATTTGATGAG TCCTCTAGAT CGAATTGCAA ATGTCACAAT CCTAAAGCAA 
CATGAAGTCG ACAAACTATA CAAAGTTGAG CTTAAACCAA TCGTCAGCAG TTCAGACCAG 
CTGTGCTTCC TCATACGTCC TAGAATACAA ACTGTGAAGT GGATTTCAGA TTTAGTTAAT 
TCAGACAAGG TAGCTGGACG ATTCCGAAGA TATAAGATCA TATTTACCCC ACAAAAGTTT 
TATGCCTGTG AAACAGTTTT GGAGGAACAG GGTGTCTATG GAGATGTTAC AACCGATGAA 
TGGAATTTCT ACATTCTTCC TCTGGATGAC GATATCCTCA GCTTAGAGCT GCCAGAGTTC 
TTCAGGGATA ACTTTCTGGA GGGAGACCAG CGCTGGGTGA CCACTGGAGG GGGTGCTTTA 
CATCTTTTAC AGTCTGTCTA TGGTTCCTTC TCAAAGGTTT ATGGGATTGG AAGATGTGCC 
AAGATGGTGT ATGAATCCTG GAGGGAACTA ATGGAGGAGG GTGAACAAAG AACAAGACAA 
CCTGAATTTG CCAAAGTATT TCTTATTGAC AGAGATGTGG ATTTTGTTAC TCCTCTCTGC 
TCTCAAGTGG TTTACGAGGG ATTGGTGGAT GACATTTTCA GAATAAAATG TAGCAGTGTG 
GAGTTTGGCC CTGATGTCAC TTCCTCTGAC AAGAGTATCA AAGTGATGCT TAACTCTCAA 
GATAAAGTGT TCAATGAAAT AAGGAATGAA CATTTTTCCA ATGTGTTTGG CTTTTTAAGT 
CAGAAGGCTA AAAATCTTCA GACAGCATAT GATAAACGGC GTGGGATGGA CATTCAGCAG 
ATGAAGGCGT TTGTTGCAGA TGAACTGAAA GGACTCAAAC AGGAACATCG TCTTCTTAGT 
CTTCATATTG GTGCAAGCGA ATCCATAATG AAGAAGAAAA CAAAGCAAGA TTTCCAGGAA 
TTGTTGAAAA CTGAACATTC ATTACTTGAA GGCTTTGAGA TCCGGGAATG TATTGCTTAC 
ATTGAAGAAC ATATCAACAG ACAGGTGTCG ATGATTGATA GCCTTAGACT CCTTTGTCTG 
TTGTCTATCA CAGAAAATGG CCTTCTGTCC AAAGACTATC GATCTCTTAA AGCTCAGTAC 
CTTCAGAGTT ATGGTATAGA GCACCTGCTG ACGTTTGCAA ACCTGAGGCA GCTGGGACTG 
TTGGAAGAAC AGCAAACTGG AGAAACACTG ACAGTCATGG AGAGTAAAGT AGGGAAACTT 
GTCAATGACA AGACTGCAGG GAAGCTGACG GATGCCTTCT CCTCACTGGC TAAGAAGAGC 
AATTTCCGAG CTCTGAGTAA AAGACTGGCT CTGGTGCCAA AGTCTGGTGA GGAATATGAC 
CTTCGCGTTC CACGAGACAT GGCTTATATT TTCAGCGGTG CTTACATTCC CCTGAGCTGC 
AAACTGATTG AACAGGTGCT GGAGAGAGAT GGGTGGACAG GTCTGGAGGA GGTCACACGC 
ATGCTGAATG GACAGGACTT TGCTGTAACA GGTGGCAGCA GTAGTTCAGA AGCCAGAAAC 
AAAAGTAATG GTCAAAGGAT CATTCTAGTG ATGTTCCTTG GTGGCTGCAC TTACTCTGAG 
ATCTCCGCAC TCCGATTCCT TGGCAAGGAA AGAGGTTGCA GATTCATAGT GCTCACAACA 
GCCATCACCA ACAGTGGACG GCTTCTGGAA GCTTTACTGG ATAAACATGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish models of human liver development and disease.
Comprehensive Physiology3(3)1213-30(2013 Jul)
Wilkins BJ,Pack M

Mutations in vacuolar H+ -ATPase subunits lead to biliary developmental defects in zebrafish.
Developmental biology365(2)434-44(2012 May)
EauClaire SF,Cui S,Ma L,Matous J,Marlow FL,Gupta T,Burgess HA,Abrams EW,Kapp LD,Granato M,Mullins MC,Matthews RP

Zebrafish vps33b, an ortholog of the gene responsible for human arthrogryposis-renal dysfunction-cholestasis syndrome, regulates biliary development downstream of the onecut transcription factor hnf6.
Development (Cambridge, England)132(23)5295-306(2005 Dec)
Matthews RP,Plumb-Rudewiez N,Lorent K,Gissen P,Johnson CA,Lemaigre F,Pack M