nubp1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nubp1
Entrez Gene ID	503919
Full Name	nucleotide binding protein 1 (MinD homolog, E. coli)
Synonyms	im:7144207,zgc:92138
General protein information	Preferred Names nucleotide binding protein 1 (MinD homolog, E. coli) Names cytosolic Fe-S cluster assembly factor nubp1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009299428.2	XP_009297703.1	cytosolic Fe-S cluster assembly factor nubp1 isoform X1
NM_001017538.2	NP_001017538.1	cytosolic Fe-S cluster assembly factor nubp1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NUBP1	NP_002475.2
Danio rerio (zebrafish)	nubp1	NP_001017538.1
Caenorhabditis elegans (roundworm)	F10G8.6	NP_492653.2
Saccharomyces cerevisiae (baker's yeast)	NBP35	NP_011424.3
Schizosaccharomyces pombe (fission yeast)	SPAC637.08	NP_594626.1
Mus musculus (house mouse)	Nubp1	NP_036085.1
Anopheles gambiae (African malaria mosquito)	NUBP1_ANOGA	XP_320535.3
Kluyveromyces lactis	KLLA0E18987g	XP_454809.1
Oryza sativa (rice)	Os02g0601100	NP_001047347.1
Bos taurus (cattle)	NUBP1	NP_001068763.1
Gallus gallus (chicken)	NUBP1	NP_001185570.1
Rattus norvegicus (Norway rat)	Nubp1	NP_001009619.1
Drosophila melanogaster (fruit fly)	CG17904	NP_609805.1
Eremothecium gossypii	AGOS_AFL060W	NP_985488.2
Arabidopsis thaliana (thale cress)	NBP35	NP_568748.1
Xenopus tropicalis (tropical clawed frog)	nubp1	NP_001015835.1
Pan troglodytes (chimpanzee)	NUBP1	XP_001139497.1
Canis lupus familiaris (dog)	NUBP1	XP_536975.1
Magnaporthe oryzae (rice blast fungus)	MGG_06151	XP_003712030.1
Neurospora crassa	NCU08825	XP_962036.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evolution of JAK-STAT pathway components: mechanisms and role in immune system development.
Liongue C, O'Sullivan LA, Trengove MC, Ward AC
PloS one7(3)e32777(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nubp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nubp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa08309	NM_001017538.2 Latest version!	Danio rerio nucleotide binding protein 1 (MinD homolog, E. coli) (nubp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa41564	XM_009299428.2 Latest version!	Danio rerio nucleotide binding protein 1 (MinD homolog, E. coli) (nubp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nubp1 ( NM_001017538.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017538.1 Danio rerio nucleotide binding protein 1 (MinD homolog, E. coli) (nubp1), mRNA.

Price & Availability↑

CloneID	ODa08309
Clone ID Related Accession (Same CDS sequence)	NM_001017538.2
Accession Version	NM_001017538.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	cytosolic Fe-S cluster assembly factor nubp1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU695195.9 and EH504704.1. On or before Feb 13, 2016 this sequence version replaced XM_005163909.2, NM_001017538.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC064668.1, GDQH01006981.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGATG TTCCTAACGA TGCACCAGAA CACTGTCCGG GAACAAGCAG TGACCAGGCT 
GGTAAATCAT CAGCATGCCA GGGATGCCCA AACCAGAGTA TATGTGCGTC TGGTGCTACC 
AAAGCCCCTG ATCCAGCTAT AGAAGAAATC AAACAGAAAA TGACCTCAGT GAAGCACAAG 
ATTCTAGTAT TGTCTGGGAA AGGTGGTGTT GGGAAGAGCA CGTTCAGCGC ACATTTGTCT 
CATGCATTAG CAAGTGATTC TTCCAAAGAG GTGGCACTGC TGGATGTGGA CATCTGTGGG 
CCATCAATTC CAAAAATAAT GGGCTTAGAA GGGGAGCAGG TTCATCAGAG CGGCTCAGGC 
TGGTCCCCTG TGTATGTTGA AGATAACTTG GCTGTGATGT CCATCGGCTT CCTCCTCAGT 
AGTCCAGATG ATGCTGTTAT TTGGAGAGGC CCCAAAAAGA ACGGCATGAT AAAACAGTTC 
CTCAGGGATG TTGACTGGGG AGAAGTGGAC TATCTGATCG TAGACACACC TCCGGGAACA 
TCAGATGAAC ACTTGTCCAT CGTGCAGTAT CTGAGTGGCG CTGGCATCGA TGGAGCAGTC 
ATCATCACAA CACCACAGGA AGTATCTTTG CAGGATGTGA GAAAAGAGAT AAGATTCTGT 
AAAAAGGTCA ACTTGCCCAT CCTAGGTGTG ATTGAGAACA TGAGTGGATT TGTTTGTCCT 
AAATGCAAGA ACACATCGCA GATATTTCCT CCGACCACAG GAGGAGCACA GAGAATGTGT 
GAAGAACTAA ATCTCCCTCT TTTGGGCAGA ATTCCCCTTG ACCCACGGAT AGGGAAAAGT 
TGTGACGAAG GAAAGTCATT CCTGACTGAA GTTCCAGACT CTCCTGCAGC AGCGGCCTAT 
CAGAGCATAG TTCAGAAAAT CAGAGACTAC TGCGCTTCAC ATTCTGCATC AGATGTTTCT 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017538.1
CDS	30..995
Translation	MADVPNDAPEHCPGTSSDQAGKSSACQGCPNQSICASGATKAPDPAIEEIKQKMTSVKHKILVLSGKGGVGKSTFSAHLSHALASDSSKEVALLDVDICGPSIPKIMGLEGEQVHQSGSGWSPVYVEDNLAVMSIGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGEVDYLIVDTPPGTSDEHLSIVQYLSGAGIDGAVIITTPQEVSLQDVRKEIRFCKKVNLPILGVIENMSGFVCPKCKNTSQIFPPTTGGAQRMCEELNLPLLGRIPLDPRIGKSCDEGKSFLTEVPDSPAAAAYQSIVQKIRDYCASHSASDVSC

Target ORF information:

RefSeq Version	NM_001017538.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleotide binding protein 1 (MinD homolog, E. coli) (nubp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017538.2

ORF Insert Sequence:

ATGGCGGATG TTCCTAACGA TGCACCAGAA CACTGTCCGG GAACAAGCAG TGACCAGGCT 
GGTAAATCAT CAGCATGCCA GGGATGCCCA AACCAGAGTA TATGTGCGTC TGGTGCTACC 
AAAGCCCCTG ATCCAGCTAT AGAAGAAATC AAACAGAAAA TGACCTCAGT GAAGCACAAG 
ATTCTAGTAT TGTCTGGGAA AGGTGGTGTT GGGAAGAGCA CGTTCAGCGC ACATTTGTCT 
CATGCATTAG CAAGTGATTC TTCCAAAGAG GTGGCACTGC TGGATGTGGA CATCTGTGGG 
CCATCAATTC CAAAAATAAT GGGCTTAGAA GGGGAGCAGG TTCATCAGAG CGGCTCAGGC 
TGGTCCCCTG TGTATGTTGA AGATAACTTG GCTGTGATGT CCATCGGCTT CCTCCTCAGT 
AGTCCAGATG ATGCTGTTAT TTGGAGAGGC CCCAAAAAGA ACGGCATGAT AAAACAGTTC 
CTCAGGGATG TTGACTGGGG AGAAGTGGAC TATCTGATCG TAGACACACC TCCGGGAACA 
TCAGATGAAC ACTTGTCCAT CGTGCAGTAT CTGAGTGGCG CTGGCATCGA TGGAGCAGTC 
ATCATCACAA CACCACAGGA AGTATCTTTG CAGGATGTGA GAAAAGAGAT AAGATTCTGT 
AAAAAGGTCA ACTTGCCCAT CCTAGGTGTG ATTGAGAACA TGAGTGGATT TGTTTGTCCT 
AAATGCAAGA ACACATCGCA GATATTTCCT CCGACCACAG GAGGAGCACA GAGAATGTGT 
GAAGAACTAA ATCTCCCTCT TTTGGGCAGA ATTCCCCTTG ACCCACGGAT AGGGAAAAGT 
TGTGACGAAG GAAAGTCATT CCTGACTGAA GTTCCAGACT CTCCTGCAGC AGCGGCCTAT 
CAGAGCATAG TTCAGAAAAT CAGAGACTAC TGCGCTTCAC ATTCTGCATC AGATGTTTCT 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nubp1 ( XM_009299428.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297703.1 Danio rerio nucleotide binding protein 1 (MinD homolog, E. coli) (nubp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41564
Clone ID Related Accession (Same CDS sequence)	XM_009299428.2
Accession Version	XM_009299428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cytosolic Fe-S cluster assembly factor nubp1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTAG AAGGGGAGCA GGTTCATCAG AGCGGCTCAG GCTGGTCCCC TGTGTATGTT 
GAAGATAACT TGGCTGTGAT GTCCATCGGC TTCCTCCTCA GTAGTCCAGA TGATGCTGTT 
ATTTGGAGAG GCCCCAAAAA GAACGGCATG ATAAAACAGT TCCTCAGGGA TGTTGACTGG 
GGAGAAGTGG ACTATCTGAT CGTAGACACA CCTCCGGGAA CATCAGATGA ACACTTGTCC 
ATCGTGCAGT ATCTGAGTGG CGCTGGCATC GATGGAGCAG TCATCATCAC AACACCACAG 
GAAGTATCTT TGCAGGATGT GAGAAAAGAG ATAAGATTCT GTAAAAAGGT CAACTTGCCC 
ATCCTAGGTG TGATTGAGAA CATGAGTGGA TTTGTTTGTC CTAAATGCAA GAACACATCG 
CAGATATTTC CTCCGACCAC AGGAGGAGCA CAGAGAATGT GTGAAGAACT AAATCTCCCT 
CTTTTGGGCA GAATTCCCCT TGACCCACGG ATAGGGAAAA GTTGTGACGA AGGAAAGTCA 
TTCCTGACTG AAGTTCCAGA CTCTCCTGCA GCAGCGGCCT ATCAGAGCAT AGTTCAGAAA 
ATCAGAGACT ACTGCGCTTC ACATTCTGCA TCAGATGTTT CTTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297703.1
CDS	373..1020
Translation	MGLEGEQVHQSGSGWSPVYVEDNLAVMSIGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGEVDYLIVDTPPGTSDEHLSIVQYLSGAGIDGAVIITTPQEVSLQDVRKEIRFCKKVNLPILGVIENMSGFVCPKCKNTSQIFPPTTGGAQRMCEELNLPLLGRIPLDPRIGKSCDEGKSFLTEVPDSPAAAAYQSIVQKIRDYCASHSASDVSC

Target ORF information:

RefSeq Version	XM_009299428.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleotide binding protein 1 (MinD homolog, E. coli) (nubp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299428.2

ORF Insert Sequence:

ATGGGCTTAG AAGGGGAGCA GGTTCATCAG AGCGGCTCAG GCTGGTCCCC TGTGTATGTT 
GAAGATAACT TGGCTGTGAT GTCCATCGGC TTCCTCCTCA GTAGTCCAGA TGATGCTGTT 
ATTTGGAGAG GCCCCAAAAA GAACGGCATG ATAAAACAGT TCCTCAGGGA TGTTGACTGG 
GGAGAAGTGG ACTATCTGAT CGTAGACACA CCTCCGGGAA CATCAGATGA ACACTTGTCC 
ATCGTGCAGT ATCTGAGTGG CGCTGGCATC GATGGAGCAG TCATCATCAC AACACCACAG 
GAAGTATCTT TGCAGGATGT GAGAAAAGAG ATAAGATTCT GTAAAAAGGT CAACTTGCCC 
ATCCTAGGTG TGATTGAGAA CATGAGTGGA TTTGTTTGTC CTAAATGCAA GAACACATCG 
CAGATATTTC CTCCGACCAC AGGAGGAGCA CAGAGAATGT GTGAAGAACT AAATCTCCCT 
CTTTTGGGCA GAATTCCCCT TGACCCACGG ATAGGGAAAA GTTGTGACGA AGGAAAGTCA 
TTCCTGACTG AAGTTCCAGA CTCTCCTGCA GCAGCGGCCT ATCAGAGCAT AGTTCAGAAA 
ATCAGAGACT ACTGCGCTTC ACATTCTGCA TCAGATGTTT CTTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evolution of JAK-STAT pathway components: mechanisms and role in immune system development.
PloS one7(3)e32777(2012)
Liongue C,O'Sullivan LA,Trengove MC,Ward AC