tln1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tln1
Entrez Gene ID	494453
Full Name	talin 1
Synonyms	sb:cb854,wu:fb05c02
General protein information	Preferred Names talin 1 Names talin-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155499.3	XP_005155556.1	talin-1 isoform X1
XM_021479168.1	XP_021334843.1	talin-1 isoform X3
NM_001009560.1	NP_001009560.1	talin-1
XM_005155500.4	XP_005155557.1	talin-1 isoform X4
XM_005155500.3	XP_005155557.1	talin-1 isoform X2
XM_021479167.1	XP_021334842.1	talin-1 isoform X1
XM_005155499.4	XP_005155556.1	talin-1 isoform X2

Pathways from BioSystems

Homologies

	TLN1	XP_001084941.2
Gallus gallus (chicken)	TLN1	NP_989854.1
Pan troglodytes (chimpanzee)	TLN1	XP_003312119.1
Canis lupus familiaris (dog)	TLN1	XP_005626844.1
Homo sapiens (human)	TLN1	NP_006280.3
Bos taurus (cattle)	TLN1	NP_001192357.1
Mus musculus (house mouse)	Tln1	NP_035732.2
Rattus norvegicus (Norway rat)	Tln1	NP_001034114.2
Danio rerio (zebrafish)	tln1	NP_001009560.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

The hyaloid vasculature facilitates basement membrane breakdown during choroid fissure closure in the zebrafish eye.
James A, Lee C, Williams AM, Angileri K, Lathrop KL, Gross JM
Developmental biology419(2)262-272(2016 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

suggest that Tln1-mediated Itgbeta1b plays a crucial role in maintaining cardiac sarcomeric Z-disks and endothelial/endocardial cell integrity in zebrafish
Title: Talin1 is required for cardiac Z-disk stabilization and endothelial integrity in zebrafish.

The following tln1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tln1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa147612	XM_021479167.1 Latest version!	Danio rerio talin 1 (tln1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa147613	XM_021479168.1 Latest version!	Danio rerio talin 1 (tln1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa32586	NM_001009560.1 Latest version!	Danio rerio talin 1 (tln1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa15805	XM_005155499.4 Latest version!	Danio rerio talin 1 (tln1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa15806	XM_005155500.4 Latest version!	Danio rerio talin 1 (tln1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
ODa15806	XM_005155500.3	Danio rerio talin 1 (tln1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa15805	XM_005155499.3	Danio rerio talin 1 (tln1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tln1 ( XM_021479167.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334842.1 Danio rerio talin 1 (tln1), transcript variant X1, mRNA.

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CloneID	ODa147612
Clone ID Related Accession (Same CDS sequence)	XM_021479167.1
Accession Version	XM_021479167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	talin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGTCTGT AGGTACAGCA GCGTTCACAC ACTCCAGACG GACAGGCTAT 
AGTCAAATGC TGGACATGTT GATGCAGTTG GCGAAGGCTG TGGCGAATGC AGCAGCGGCT 
CTGGTGTTAA AGGCTAAAAA CGTGGCTCAG AAAAGCGAAG ATTCAGCTCA ACAAAACAAA 
GTGATCGCTG CTGCCACCCA GTGCGCCCTC TCCACATCAC AGCTGGTGGC CTGCACACGA 
GTGGTGGCTC CAACCATCAG CTCACCCGTG TGTCAAGAGC AGCTCATCGA GGCTGGAAAA 
CTGGTGGCGA AGTCTGTGGA GGGCTGTGTG GAGGCGTCAC ACGGGGCGAC CGGTGACGAG 
CATCTGCTCA AACAGGTGGG CGCCGCTGCC ACAGGAGTCA CTCAGGCTCT TAACGAGCTG 
CTGCAGCATA TTAGACAGTA CGCCAGCGGA GGACAGCCAA TCGGACGGCA CGGAGAGGCT 
ACCGACCGCA TCCTGGATGT GACTGATAAC ATCTTCAGCT CCATGGGAGA TGCAGGGGAG 
ATGGTGCGTC AGGCGCGGAT CCTTGCTCAG GCCACCTCAG ACCTGGTCAA CGCGATCAAG 
GCGGACGCAG AGGGAGAATC TGACCTGGAG AACTCTCGCA AACTCCTCTC TGCAGCCAAA 
CTCCTGGCTG ACGCTACTGC CCGTATGGTG GAAGCTGCGA AGGGTGCAGC TGCTAATCCA 
GACAGCGAGG AGCAGCAGCA GAAGTTGCGT GAAGCTGCAG AGGGACTGCG CATGGCCACC 
AATGCTGCAG CACAGAACGC CATTAAAAAG AGACTCGTCA ACAAACTGGA GAATGCAGCG 
AAACAAGCCG CAGCAGCCGC CACTCAGACC ATAGCAGCCG CTCAACATGC AGCTTCATCC 
AACAAGAACC CTGCGGCCCA GCAACAACTC GTACAGAGCT GCAAGGTGGT GGCGGATCAG 
ATCCCACAGC TGGTTCAGGG TCTGAGGAGC AGCCAGGCTC AACCTGACAG CCCCAGCGCT 
CAACTCGCCC TCATCGGAGC TAGCCAGAAC TTCCTGCAGC CCGGAGCCAA AATGGTGACC 
GCAGCCAAAG CTACAGTTCC AACCATCAGT GACCAGGCGT CTGCCATGCA GCTCAGTCAG 
TGCGCTAAAA ACCTGGCCAG TGCTCTGGCT GAGCTCCGCA CGGCCGCCCA GAAGGCTCAG 
GAGGCGTGTG GTCCGTTGGA GATTGATAAC GCTCTGACTA TGGTACGGGG GCTGGAGAGG 
GACATGCAGG AGGCCAAAGC TTCAGCTGCT GAGGGCAAAC TCAGACCCCT GCCTGGAGAA 
ACGCTGGAGA AGTGCTCTCA GGATCTGGGC TCCAGCACTA AAGCCGTGAG CTCTGCCATT 
GCTCAGCTCC TCAGTGAAGC AACTCAAGGC AATGAGAACT ACACAGGTAT GGCTGCTCGT 
GATGTGGCTC AGGCTCTGCG CTCGCTGGCT TCAGCCGCTC GTGGAGTGGC AGCCAACACT 
GAAGATCCTC TAGCCCGTAA CGCAATGCTG GACTGCACCG GCGATGTCAT GGACAAATCC 
GCCAGCCTGA TTGAAGAGAC CAAGAGGGCC ATAGCCAAAC CAGGAGACCC TGACAGTCAG 
CAAAGACTGG CCCAGGTAGC CAAGGCTGTG TCTCAGGCAC TGAACCGCTC TGTAAACTGT 
CTGCCGGGGC AGAGGGATGT GGATAATGCC ATCAGAACTG TCGGGGAAGC TAGCAAGAAA 
CTGCTGTCTG ATAAATTTCC AGCGAGTGGG AAGAATTTCC AAGAGGCTCA GGCCAATCTG 
AACGAGGCTG CAGCTGGACT AAACCAGTCA GCCAATGAGC TGGTGCAGGC CTCGCGTGGC 
ACCACCCAGG ACCTGGCCAA AGCCTCCGGG AAGTTTGGAC AAGACTTTAA CCAATTCCTG 
CAGGCTGGAG TGGACATGGC TGGACAATCC CAGTCTAAAG AAGACCAAAC TCAAGTGGTC 
ACCAACCTGA AGACCATCTC CATGTCCTCC AGCAAACTCC TGCTGGCGGC TAAAGCTCTG 
TCCACAGACC CCAACTCGCC AAATCTGAAG AACCAACTCG CTGCTGCTGC TCGCGCGGTG 
ACAGACAGCA TTAATCAGCT GATCAGCATG TGTACCCAGC AGGCTCCGGG ACAGAAGGAG 
TGTGACAACG CTCTCCGTGA GCTTGAGACG GTTGGAGGAA TGCTGGAGAA CCCAACAGAA 
GCAGTCAATG ATTTGGGCTA CTTTGACTGC ATTGACAGTG TGATGGAGAA CTCAAAGGTT 
TTGGGTGAGG CCATGGCTGG CATTTCCCAT AATGCCAAAA ACAGTAACTT GCCAGAGTTT 
GGAGATTCGG TCAGTTCAGC GTCTAAAGCG CTGTGTGGTC TCACTGAAGC AGCTGCTCAG 
GGGGCGTATC TGGTTGGTGT GTCAGACCCG AACAGCCATG CTGGCCAGAA GGGTCTTGTG 
GACCCCTCAC AATTTGCCAG AGCCAAACAG TCCATCCAGA TGGCCTGTCA GAACCTGGTG 
GATCCAGCCT GCACCCAGTC CCAGGTTCTC TCTGCTGCCA CCATCGTTGC CAAACACACT 
TCGGCCTTGT GCAATGCCTG CCGGCTCGCC TCCTCCAAGA CATCCAACCC AGTTGCTAAG 
AGACAGTTCG TCCAGAGTGC CAAAGAGGTG GCCAACAGCA CAGCCAACCT CGTCAAGTCC 
ATCAAGGCTC TCGACGGGGC CTTTAACCAG GAAAACAGAC AGAAGTGTAA GGAAGCCACT 
GGGCCACTCA TTGAGGCTGT GGATAACCTG ACTGCCTTCG CTTCAAACCC AGAGTTCGCA 
AGTGTACCCG CTCAGATCAG CCCAGAGGGT CTGGCTGCGA TGGAGCCGAT CACAGCAGCC 
GCTAAGGCAA TGTTGGAAAG CTCGACAGGA CTGATCCAGA CGGCTCGCTC GCTCGCAGTC 
AACCCGAAAG ATCCACCCAA GTGGTCTGTG CTTGCTGGAC ACTCCAGAAC AGTGTCCGAC 
TCCATCAAGA AACTCATCAC TAACATGAGG GAAAAGGCTC CGGGTCAGAG GGAGTGTGAT 
GATGCCATTG AGGTTCTTAG TAACTGCATT CGTGAAGTGG ACAAGGCCTC GCTGGCAGCC 
ATCAGTCAGC AGCTCACGCC TAGAGACGAC ATTTCCCATG AGGCTCTGCA CGAGCAGATG 
GCGGCGTCTG TGCATGAGAT CAGTAACCTG ATTGAACCAG TGGCCATCGC AGCCCGTGCT 
GATGCCTCTC AGCTGGGACA CAAGGTGTCT CAGATGGTCA GTTACTTTGA ACCGCTCATC 
ATGGCTGCCA TCGGCACAGC GTCTAAGATC CTGAACAGCC AGCAGCAGAT GAACGTGCTG 
GACCAAACCA AGACTCTGGC TGAGTCTGCG CTACAGATGC TCTATACGGC CAAAGAGACT 
GGAGGAAACC ACAAGGCGGC GCACACACAA GAGGCTCTGG AGGAGTCTGT TCAGATGATG 
AAGGAGGCGG TGGACGATCT GGGTTCGACT CTGGCAGAAG TGGCCAGCGC TGCAGGAGCA 
GTGGGAGGAA TGGTCAACTC CATCACTCAG TCCATCAACA AGATGGAGGA CGGACCTGCC 
TTCGAGCCTG AAGGAACGTT TGTGGATTAT CAGACCACCA TGGTGAAGAC AGCCAAAGCT 
ATCGCTGTCA CCGTTCAGGA GATGGTTACC AAATCCAACA CCAACCCTGA TGAGCTGGGC 
GGTTTGGCCA ATCAGCTGAC CACAGAGTTC GGTGACCTCG CATCAGAGGC CAAATGTGCA 
GCCATGACTG CTGAAAACGA TGAGATCGGT TCACATATCA AGAAGCAGGT GACTGAGTTG 
GGCTACACCT GCACTGGTCT GGTCAGTAAA GCAGGAGCGC TACAGTGCAG TCCAAACGAC 
TCCTACACTA AGAAGGAGCT CATTGACAGT GCCAGGAAAG TCTCTGAGAA GGTCTCTCAT 
GTGCTGGCAG CTCTTCAGGC AGGCAATCGT GGCACTCAGG CCTGCATTAC TGCCGCGAGC 
GCTGTCTCTG GCATCATTGC TGATTTAGAC ACCACCATCA TGTTTGCCAC CGCGGGCACT 
CTCAACCGAG AGAACGCAGA GACATTTGCA GACCACAGAG AGCACATCTT AAAGACGGCT 
AAAGTTCTGG TGGAGGACAC GAAGCTGCTG GTTTCTGGAG CCGGAGCAAG TCAGGAGAAA 
CTGGCTCAGG CTGCACAGTC CTCCGTCAAC ACCATCACCA AACTGGCCGA TGTGGTTAAA 
TTGGGGGCAG CGAGTCTGGG CTCTGAAGAC CCAGAGACAC AGGTGGTTCT GATCAACGCT 
GTGAAGGACG TGGCTAAAGC TCTGGGTGAC CTGATCAGCG CCACCAAAGC AGCGGCCGGC 
AAACCTCACG ATGACCCCGC CATGCTGCAG CTCAAAAACT CTGCTAAGGT GATGGTGACC 
AATGTGACGT CTCTACTTAA GACGGTGAAG GCGGTGGAGG ATGAAGCCAC TAAAGGCACA 
CGAGCACTAG AGGCCACCAT AGAGCACATC AAACAAGAGC TGGCTGTTTT CAGCAGTGCA 
GATCCTCCTC CAAAGACGGC CACTCCAGAG GAGTTCATCC GCATGACCAA AGGCATCACA 
TTGGCCACAG CTAAAGCAGT GGCTGCTGGG AACTCCTGCC GTCAGGAGGA CGTGATCGCC 
ACTGCTAACC TAAGCAGACG AGCTGTCGCA GACATGCTGC GCTCCTGCAA GCAAGCCGCA 
TACCACCCGG AGGTGAAGCG GGAGGTCCAG ACTCGAGCGC TCCGCTTTGG CTCTGAGTGC 
TCCACAAGTT ACCTGGGCCT GCTGGAGCAC GTGTTAGTGC CGATCATTCA GAAACCCTCG 
CATGACCTTA AACAGCAGCT GGCAAACTAC TCCAAACGTG TGGCCAGTTC AGTGACCGAG 
CTCATCCAGG CTGCGGAGGC CATGAAAGGT ACGGAGTGGG TGGATCCCGA AGACCCCACC 
GTCATTGCAG AAAACGAGCT GCTGGGAGCC GCAGCTGCCA TTGAAGCTGC CGCGAAGAAA 
CTAGAGCAGC TCAAACCCCG AGCCAAACCC AAGGAAGCTG ATGAGAGTCT GAACTTTGAG 
GAACAGATTC TAGAAGCGGC CAAATCCATC GCTGCAGCCA CCAGTGCTCT GGTCAAAGCT 
GCGTCTGCCG CTCAGAGAGA GCTGGTGGCT CAAGGGAAGG TTGGGGCAAT TCCAGCAAAC 
GCGGTGGATG ATGGACAGTG GTCTCAGGGT CTCATTTCAG CTGCTCGTAT GGTAGCCGCA 
GCTACTAACA ACCTGTGCGA AGCAGCTAAC TCTGCGGTTC AGGGTCACGC CAGCGAGGAG 
AAACTCATTT CTTCAGCCAA ACAGGTGGCA GCATCCACCG CACAGCTGCT GGTGGCCTGT 
AAGGTCAAGG CTGACCAGGA CTCCCAGACC ATGAAGAGAC TGCAGGCTGC TGGTAATGCT 
GTCAAGAGGG CGTCTGATAA CCTGGTGAAG GCGGCACAGA AGGCTGCGTT TGAGGCTCAA 
GATGATCAGG CTGTGATGGT GAAGAGCAAG ATGGTGGGCG GTATTGCTCA GATCATCGCT 
GCTCAGGAGG AGATGCTCAG GAAGGAGAAA GAGCTGGAAG AAGCCAGAAA GAAACTGGCA 
ATGATCCGAC AGCAGCAGTA CAAGTTCCTG CCCTCAGAGC TGCGAGAGGA CGGACAGGAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334842.1
CDS	203..7888
Translation	MVALSLKIGVGNVVKTMQFEPSTVVYDACRIIRERVPEAQLGQPSDYGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTLEYKKKQRPLKIRMLDGTVKTVMVDDSKIVSDMLMTICARIGITNYDEYSLVRDVGEEKKEETTGTLKRDKTLLRDDKKMEKLKQKLHTDDELNWLDHGRTLREQGVEESEMLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGYQCQIQFGDHNESKHKPGFLDLKEFLPKEYVKNKGEKRIFQAHKNCQNMTEIEAKVNYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKESVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQFNKMGKVETGSVALPAIIRSGAGGPESMKMGSMPQAKQHITSGQMHRGHMPPLTSAQQALTGTINSSMQAVQAVQASLDEFDALPPLGTDSASQAWRKNKMDESKHEIHSQVDAITAGTASMVNLTAGDPAETDYTAVGCAVTTISSNLTEMSKGVKLLAALMEDEGGNGQQLLGAAKNLACAVSDMLKTAQPSSTEPRQNLLQAAGNVGQASGELLQQIGESDADQHFQSVGTAAFTHSRRTGYSQMLDMLMQLAKAVANAAAALVLKAKNVAQKSEDSAQQNKVIAAATQCALSTSQLVACTRVVAPTISSPVCQEQLIEAGKLVAKSVEGCVEASHGATGDEHLLKQVGAAATGVTQALNELLQHIRQYASGGQPIGRHGEATDRILDVTDNIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGESDLENSRKLLSAAKLLADATARMVEAAKGAAANPDSEEQQQKLREAAEGLRMATNAAAQNAIKKRLVNKLENAAKQAAAAATQTIAAAQHAASSNKNPAAQQQLVQSCKVVADQIPQLVQGLRSSQAQPDSPSAQLALIGASQNFLQPGAKMVTAAKATVPTISDQASAMQLSQCAKNLASALAELRTAAQKAQEACGPLEIDNALTMVRGLERDMQEAKASAAEGKLRPLPGETLEKCSQDLGSSTKAVSSAIAQLLSEATQGNENYTGMAARDVAQALRSLASAARGVAANTEDPLARNAMLDCTGDVMDKSASLIEETKRAIAKPGDPDSQQRLAQVAKAVSQALNRSVNCLPGQRDVDNAIRTVGEASKKLLSDKFPASGKNFQEAQANLNEAAAGLNQSANELVQASRGTTQDLAKASGKFGQDFNQFLQAGVDMAGQSQSKEDQTQVVTNLKTISMSSSKLLLAAKALSTDPNSPNLKNQLAAAARAVTDSINQLISMCTQQAPGQKECDNALRELETVGGMLENPTEAVNDLGYFDCIDSVMENSKVLGEAMAGISHNAKNSNLPEFGDSVSSASKALCGLTEAAAQGAYLVGVSDPNSHAGQKGLVDPSQFARAKQSIQMACQNLVDPACTQSQVLSAATIVAKHTSALCNACRLASSKTSNPVAKRQFVQSAKEVANSTANLVKSIKALDGAFNQENRQKCKEATGPLIEAVDNLTAFASNPEFASVPAQISPEGLAAMEPITAAAKAMLESSTGLIQTARSLAVNPKDPPKWSVLAGHSRTVSDSIKKLITNMREKAPGQRECDDAIEVLSNCIREVDKASLAAISQQLTPRDDISHEALHEQMAASVHEISNLIEPVAIAARADASQLGHKVSQMVSYFEPLIMAAIGTASKILNSQQQMNVLDQTKTLAESALQMLYTAKETGGNHKAAHTQEALEESVQMMKEAVDDLGSTLAEVASAAGAVGGMVNSITQSINKMEDGPAFEPEGTFVDYQTTMVKTAKAIAVTVQEMVTKSNTNPDELGGLANQLTTEFGDLASEAKCAAMTAENDEIGSHIKKQVTELGYTCTGLVSKAGALQCSPNDSYTKKELIDSARKVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNRENAETFADHREHILKTAKVLVEDTKLLVSGAGASQEKLAQAAQSSVNTITKLADVVKLGAASLGSEDPETQVVLINAVKDVAKALGDLISATKAAAGKPHDDPAMLQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATIEHIKQELAVFSSADPPPKTATPEEFIRMTKGITLATAKAVAAGNSCRQEDVIATANLSRRAVADMLRSCKQAAYHPEVKREVQTRALRFGSECSTSYLGLLEHVLVPIIQKPSHDLKQQLANYSKRVASSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATNNLCEAANSAVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSQTMKRLQAAGNAVKRASDNLVKAAQKAAFEAQDDQAVMVKSKMVGGIAQIIAAQEEMLRKEKELEEARKKLAMIRQQQYKFLPSELREDGQEQ

Target ORF information:

RefSeq Version	XM_021479167.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio talin 1 (tln1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479167.1

ORF Insert Sequence:

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGTCTGT AGGTACAGCA GCGTTCACAC ACTCCAGACG GACAGGCTAT 
AGTCAAATGC TGGACATGTT GATGCAGTTG GCGAAGGCTG TGGCGAATGC AGCAGCGGCT 
CTGGTGTTAA AGGCTAAAAA CGTGGCTCAG AAAAGCGAAG ATTCAGCTCA ACAAAACAAA 
GTGATCGCTG CTGCCACCCA GTGCGCCCTC TCCACATCAC AGCTGGTGGC CTGCACACGA 
GTGGTGGCTC CAACCATCAG CTCACCCGTG TGTCAAGAGC AGCTCATCGA GGCTGGAAAA 
CTGGTGGCGA AGTCTGTGGA GGGCTGTGTG GAGGCGTCAC ACGGGGCGAC CGGTGACGAG 
CATCTGCTCA AACAGGTGGG CGCCGCTGCC ACAGGAGTCA CTCAGGCTCT TAACGAGCTG 
CTGCAGCATA TTAGACAGTA CGCCAGCGGA GGACAGCCAA TCGGACGGCA CGGAGAGGCT 
ACCGACCGCA TCCTGGATGT GACTGATAAC ATCTTCAGCT CCATGGGAGA TGCAGGGGAG 
ATGGTGCGTC AGGCGCGGAT CCTTGCTCAG GCCACCTCAG ACCTGGTCAA CGCGATCAAG 
GCGGACGCAG AGGGAGAATC TGACCTGGAG AACTCTCGCA AACTCCTCTC TGCAGCCAAA 
CTCCTGGCTG ACGCTACTGC CCGTATGGTG GAAGCTGCGA AGGGTGCAGC TGCTAATCCA 
GACAGCGAGG AGCAGCAGCA GAAGTTGCGT GAAGCTGCAG AGGGACTGCG CATGGCCACC 
AATGCTGCAG CACAGAACGC CATTAAAAAG AGACTCGTCA ACAAACTGGA GAATGCAGCG 
AAACAAGCCG CAGCAGCCGC CACTCAGACC ATAGCAGCCG CTCAACATGC AGCTTCATCC 
AACAAGAACC CTGCGGCCCA GCAACAACTC GTACAGAGCT GCAAGGTGGT GGCGGATCAG 
ATCCCACAGC TGGTTCAGGG TCTGAGGAGC AGCCAGGCTC AACCTGACAG CCCCAGCGCT 
CAACTCGCCC TCATCGGAGC TAGCCAGAAC TTCCTGCAGC CCGGAGCCAA AATGGTGACC 
GCAGCCAAAG CTACAGTTCC AACCATCAGT GACCAGGCGT CTGCCATGCA GCTCAGTCAG 
TGCGCTAAAA ACCTGGCCAG TGCTCTGGCT GAGCTCCGCA CGGCCGCCCA GAAGGCTCAG 
GAGGCGTGTG GTCCGTTGGA GATTGATAAC GCTCTGACTA TGGTACGGGG GCTGGAGAGG 
GACATGCAGG AGGCCAAAGC TTCAGCTGCT GAGGGCAAAC TCAGACCCCT GCCTGGAGAA 
ACGCTGGAGA AGTGCTCTCA GGATCTGGGC TCCAGCACTA AAGCCGTGAG CTCTGCCATT 
GCTCAGCTCC TCAGTGAAGC AACTCAAGGC AATGAGAACT ACACAGGTAT GGCTGCTCGT 
GATGTGGCTC AGGCTCTGCG CTCGCTGGCT TCAGCCGCTC GTGGAGTGGC AGCCAACACT 
GAAGATCCTC TAGCCCGTAA CGCAATGCTG GACTGCACCG GCGATGTCAT GGACAAATCC 
GCCAGCCTGA TTGAAGAGAC CAAGAGGGCC ATAGCCAAAC CAGGAGACCC TGACAGTCAG 
CAAAGACTGG CCCAGGTAGC CAAGGCTGTG TCTCAGGCAC TGAACCGCTC TGTAAACTGT 
CTGCCGGGGC AGAGGGATGT GGATAATGCC ATCAGAACTG TCGGGGAAGC TAGCAAGAAA 
CTGCTGTCTG ATAAATTTCC AGCGAGTGGG AAGAATTTCC AAGAGGCTCA GGCCAATCTG 
AACGAGGCTG CAGCTGGACT AAACCAGTCA GCCAATGAGC TGGTGCAGGC CTCGCGTGGC 
ACCACCCAGG ACCTGGCCAA AGCCTCCGGG AAGTTTGGAC AAGACTTTAA CCAATTCCTG 
CAGGCTGGAG TGGACATGGC TGGACAATCC CAGTCTAAAG AAGACCAAAC TCAAGTGGTC 
ACCAACCTGA AGACCATCTC CATGTCCTCC AGCAAACTCC TGCTGGCGGC TAAAGCTCTG 
TCCACAGACC CCAACTCGCC AAATCTGAAG AACCAACTCG CTGCTGCTGC TCGCGCGGTG 
ACAGACAGCA TTAATCAGCT GATCAGCATG TGTACCCAGC AGGCTCCGGG ACAGAAGGAG 
TGTGACAACG CTCTCCGTGA GCTTGAGACG GTTGGAGGAA TGCTGGAGAA CCCAACAGAA 
GCAGTCAATG ATTTGGGCTA CTTTGACTGC ATTGACAGTG TGATGGAGAA CTCAAAGGTT 
TTGGGTGAGG CCATGGCTGG CATTTCCCAT AATGCCAAAA ACAGTAACTT GCCAGAGTTT 
GGAGATTCGG TCAGTTCAGC GTCTAAAGCG CTGTGTGGTC TCACTGAAGC AGCTGCTCAG 
GGGGCGTATC TGGTTGGTGT GTCAGACCCG AACAGCCATG CTGGCCAGAA GGGTCTTGTG 
GACCCCTCAC AATTTGCCAG AGCCAAACAG TCCATCCAGA TGGCCTGTCA GAACCTGGTG 
GATCCAGCCT GCACCCAGTC CCAGGTTCTC TCTGCTGCCA CCATCGTTGC CAAACACACT 
TCGGCCTTGT GCAATGCCTG CCGGCTCGCC TCCTCCAAGA CATCCAACCC AGTTGCTAAG 
AGACAGTTCG TCCAGAGTGC CAAAGAGGTG GCCAACAGCA CAGCCAACCT CGTCAAGTCC 
ATCAAGGCTC TCGACGGGGC CTTTAACCAG GAAAACAGAC AGAAGTGTAA GGAAGCCACT 
GGGCCACTCA TTGAGGCTGT GGATAACCTG ACTGCCTTCG CTTCAAACCC AGAGTTCGCA 
AGTGTACCCG CTCAGATCAG CCCAGAGGGT CTGGCTGCGA TGGAGCCGAT CACAGCAGCC 
GCTAAGGCAA TGTTGGAAAG CTCGACAGGA CTGATCCAGA CGGCTCGCTC GCTCGCAGTC 
AACCCGAAAG ATCCACCCAA GTGGTCTGTG CTTGCTGGAC ACTCCAGAAC AGTGTCCGAC 
TCCATCAAGA AACTCATCAC TAACATGAGG GAAAAGGCTC CGGGTCAGAG GGAGTGTGAT 
GATGCCATTG AGGTTCTTAG TAACTGCATT CGTGAAGTGG ACAAGGCCTC GCTGGCAGCC 
ATCAGTCAGC AGCTCACGCC TAGAGACGAC ATTTCCCATG AGGCTCTGCA CGAGCAGATG 
GCGGCGTCTG TGCATGAGAT CAGTAACCTG ATTGAACCAG TGGCCATCGC AGCCCGTGCT 
GATGCCTCTC AGCTGGGACA CAAGGTGTCT CAGATGGTCA GTTACTTTGA ACCGCTCATC 
ATGGCTGCCA TCGGCACAGC GTCTAAGATC CTGAACAGCC AGCAGCAGAT GAACGTGCTG 
GACCAAACCA AGACTCTGGC TGAGTCTGCG CTACAGATGC TCTATACGGC CAAAGAGACT 
GGAGGAAACC ACAAGGCGGC GCACACACAA GAGGCTCTGG AGGAGTCTGT TCAGATGATG 
AAGGAGGCGG TGGACGATCT GGGTTCGACT CTGGCAGAAG TGGCCAGCGC TGCAGGAGCA 
GTGGGAGGAA TGGTCAACTC CATCACTCAG TCCATCAACA AGATGGAGGA CGGACCTGCC 
TTCGAGCCTG AAGGAACGTT TGTGGATTAT CAGACCACCA TGGTGAAGAC AGCCAAAGCT 
ATCGCTGTCA CCGTTCAGGA GATGGTTACC AAATCCAACA CCAACCCTGA TGAGCTGGGC 
GGTTTGGCCA ATCAGCTGAC CACAGAGTTC GGTGACCTCG CATCAGAGGC CAAATGTGCA 
GCCATGACTG CTGAAAACGA TGAGATCGGT TCACATATCA AGAAGCAGGT GACTGAGTTG 
GGCTACACCT GCACTGGTCT GGTCAGTAAA GCAGGAGCGC TACAGTGCAG TCCAAACGAC 
TCCTACACTA AGAAGGAGCT CATTGACAGT GCCAGGAAAG TCTCTGAGAA GGTCTCTCAT 
GTGCTGGCAG CTCTTCAGGC AGGCAATCGT GGCACTCAGG CCTGCATTAC TGCCGCGAGC 
GCTGTCTCTG GCATCATTGC TGATTTAGAC ACCACCATCA TGTTTGCCAC CGCGGGCACT 
CTCAACCGAG AGAACGCAGA GACATTTGCA GACCACAGAG AGCACATCTT AAAGACGGCT 
AAAGTTCTGG TGGAGGACAC GAAGCTGCTG GTTTCTGGAG CCGGAGCAAG TCAGGAGAAA 
CTGGCTCAGG CTGCACAGTC CTCCGTCAAC ACCATCACCA AACTGGCCGA TGTGGTTAAA 
TTGGGGGCAG CGAGTCTGGG CTCTGAAGAC CCAGAGACAC AGGTGGTTCT GATCAACGCT 
GTGAAGGACG TGGCTAAAGC TCTGGGTGAC CTGATCAGCG CCACCAAAGC AGCGGCCGGC 
AAACCTCACG ATGACCCCGC CATGCTGCAG CTCAAAAACT CTGCTAAGGT GATGGTGACC 
AATGTGACGT CTCTACTTAA GACGGTGAAG GCGGTGGAGG ATGAAGCCAC TAAAGGCACA 
CGAGCACTAG AGGCCACCAT AGAGCACATC AAACAAGAGC TGGCTGTTTT CAGCAGTGCA 
GATCCTCCTC CAAAGACGGC CACTCCAGAG GAGTTCATCC GCATGACCAA AGGCATCACA 
TTGGCCACAG CTAAAGCAGT GGCTGCTGGG AACTCCTGCC GTCAGGAGGA CGTGATCGCC 
ACTGCTAACC TAAGCAGACG AGCTGTCGCA GACATGCTGC GCTCCTGCAA GCAAGCCGCA 
TACCACCCGG AGGTGAAGCG GGAGGTCCAG ACTCGAGCGC TCCGCTTTGG CTCTGAGTGC 
TCCACAAGTT ACCTGGGCCT GCTGGAGCAC GTGTTAGTGC CGATCATTCA GAAACCCTCG 
CATGACCTTA AACAGCAGCT GGCAAACTAC TCCAAACGTG TGGCCAGTTC AGTGACCGAG 
CTCATCCAGG CTGCGGAGGC CATGAAAGGT ACGGAGTGGG TGGATCCCGA AGACCCCACC 
GTCATTGCAG AAAACGAGCT GCTGGGAGCC GCAGCTGCCA TTGAAGCTGC CGCGAAGAAA 
CTAGAGCAGC TCAAACCCCG AGCCAAACCC AAGGAAGCTG ATGAGAGTCT GAACTTTGAG 
GAACAGATTC TAGAAGCGGC CAAATCCATC GCTGCAGCCA CCAGTGCTCT GGTCAAAGCT 
GCGTCTGCCG CTCAGAGAGA GCTGGTGGCT CAAGGGAAGG TTGGGGCAAT TCCAGCAAAC 
GCGGTGGATG ATGGACAGTG GTCTCAGGGT CTCATTTCAG CTGCTCGTAT GGTAGCCGCA 
GCTACTAACA ACCTGTGCGA AGCAGCTAAC TCTGCGGTTC AGGGTCACGC CAGCGAGGAG 
AAACTCATTT CTTCAGCCAA ACAGGTGGCA GCATCCACCG CACAGCTGCT GGTGGCCTGT 
AAGGTCAAGG CTGACCAGGA CTCCCAGACC ATGAAGAGAC TGCAGGCTGC TGGTAATGCT 
GTCAAGAGGG CGTCTGATAA CCTGGTGAAG GCGGCACAGA AGGCTGCGTT TGAGGCTCAA 
GATGATCAGG CTGTGATGGT GAAGAGCAAG ATGGTGGGCG GTATTGCTCA GATCATCGCT 
GCTCAGGAGG AGATGCTCAG GAAGGAGAAA GAGCTGGAAG AAGCCAGAAA GAAACTGGCA 
ATGATCCGAC AGCAGCAGTA CAAGTTCCTG CCCTCAGAGC TGCGAGAGGA CGGACAGGAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tln1 ( XM_021479168.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334843.1 Danio rerio talin 1 (tln1), transcript variant X3, mRNA.

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CloneID	ODa147613
Clone ID Related Accession (Same CDS sequence)	XM_021479168.1
Accession Version	XM_021479168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	talin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGGACAT GTTGATGCAG TTGGCGAAGG CTGTGGCGAA TGCAGCAGCG 
GCTCTGGTGT TAAAGGCTAA AAACGTGGCT CAGAAAAGCG AAGATTCAGC TCAACAAAAC 
AAAGTGATCG CTGCTGCCAC CCAGTGCGCC CTCTCCACAT CACAGCTGGT GGCCTGCACA 
CGAGTGGTGG CTCCAACCAT CAGCTCACCC GTGTGTCAAG AGCAGCTCAT CGAGGCTGGA 
AAACTGGTGG CGAAGTCTGT GGAGGGCTGT GTGGAGGCGT CACACGGGGC GACCGGTGAC 
GAGCATCTGC TCAAACAGGT GGGCGCCGCT GCCACAGGAG TCACTCAGGC TCTTAACGAG 
CTGCTGCAGC ATATTAGACA GTACGCCAGC GGAGGACAGC CAATCGGACG GCACGGAGAG 
GCTACCGACC GCATCCTGGA TGTGACTGAT AACATCTTCA GCTCCATGGG AGATGCAGGG 
GAGATGGTGC GTCAGGCGCG GATCCTTGCT CAGGCCACCT CAGACCTGGT CAACGCGATC 
AAGGCGGACG CAGAGGGAGA ATCTGACCTG GAGAACTCTC GCAAACTCCT CTCTGCAGCC 
AAACTCCTGG CTGACGCTAC TGCCCGTATG GTGGAAGCTG CGAAGGGTGC AGCTGCTAAT 
CCAGACAGCG AGGAGCAGCA GCAGAAGTTG CGTGAAGCTG CAGAGGGACT GCGCATGGCC 
ACCAATGCTG CAGCACAGAA CGCCATTAAA AAGAGACTCG TCAACAAACT GGAGAATGCA 
GCGAAACAAG CCGCAGCAGC CGCCACTCAG ACCATAGCAG CCGCTCAACA TGCAGCTTCA 
TCCAACAAGA ACCCTGCGGC CCAGCAACAA CTCGTACAGA GCTGCAAGGT GGTGGCGGAT 
CAGATCCCAC AGCTGGTTCA GGGTCTGAGG AGCAGCCAGG CTCAACCTGA CAGCCCCAGC 
GCTCAACTCG CCCTCATCGG AGCTAGCCAG AACTTCCTGC AGCCCGGAGC CAAAATGGTG 
ACCGCAGCCA AAGCTACAGT TCCAACCATC AGTGACCAGG CGTCTGCCAT GCAGCTCAGT 
CAGTGCGCTA AAAACCTGGC CAGTGCTCTG GCTGAGCTCC GCACGGCCGC CCAGAAGGCT 
CAGGAGGCGT GTGGTCCGTT GGAGATTGAT AACGCTCTGA CTATGGTACG GGGGCTGGAG 
AGGGACATGC AGGAGGCCAA AGCTTCAGCT GCTGAGGGCA AACTCAGACC CCTGCCTGGA 
GAAACGCTGG AGAAGTGCTC TCAGGATCTG GGCTCCAGCA CTAAAGCCGT GAGCTCTGCC 
ATTGCTCAGC TCCTCAGTGA AGCAACTCAA GGCAATGAGA ACTACACAGG TATGGCTGCT 
CGTGATGTGG CTCAGGCTCT GCGCTCGCTG GCTTCAGCCG CTCGTGGAGT GGCAGCCAAC 
ACTGAAGATC CTCTAGCCCG TAACGCAATG CTGGACTGCA CCGGCGATGT CATGGACAAA 
TCCGCCAGCC TGATTGAAGA GACCAAGAGG GCCATAGCCA AACCAGGAGA CCCTGACAGT 
CAGCAAAGAC TGGCCCAGGT AGCCAAGGCT GTGTCTCAGG CACTGAACCG CTCTGTAAAC 
TGTCTGCCGG GGCAGAGGGA TGTGGATAAT GCCATCAGAA CTGTCGGGGA AGCTAGCAAG 
AAACTGCTGT CTGATAAATT TCCAGCGAGT GGGAAGAATT TCCAAGAGGC TCAGGCCAAT 
CTGAACGAGG CTGCAGCTGG ACTAAACCAG TCAGCCAATG AGCTGGTGCA GGCCTCGCGT 
GGCACCACCC AGGACCTGGC CAAAGCCTCC GGGAAGTTTG GACAAGACTT TAACCAATTC 
CTGCAGGCTG GAGTGGACAT GGCTGGACAA TCCCAGTCTA AAGAAGACCA AACTCAAGTG 
GTCACCAACC TGAAGACCAT CTCCATGTCC TCCAGCAAAC TCCTGCTGGC GGCTAAAGCT 
CTGTCCACAG ACCCCAACTC GCCAAATCTG AAGAACCAAC TCGCTGCTGC TGCTCGCGCG 
GTGACAGACA GCATTAATCA GCTGATCAGC ATGTGTACCC AGCAGGCTCC GGGACAGAAG 
GAGTGTGACA ACGCTCTCCG TGAGCTTGAG ACGGTTGGAG GAATGCTGGA GAACCCAACA 
GAAGCAGTCA ATGATTTGGG CTACTTTGAC TGCATTGACA GTGTGATGGA GAACTCAAAG 
GTTTTGGGTG AGGCCATGGC TGGCATTTCC CATAATGCCA AAAACAGTAA CTTGCCAGAG 
TTTGGAGATT CGGTCAGTTC AGCGTCTAAA GCGCTGTGTG GTCTCACTGA AGCAGCTGCT 
CAGGGGGCGT ATCTGGTTGG TGTGTCAGAC CCGAACAGCC ATGCTGGCCA GAAGGGTCTT 
GTGGACCCCT CACAATTTGC CAGAGCCAAA CAGTCCATCC AGATGGCCTG TCAGAACCTG 
GTGGATCCAG CCTGCACCCA GTCCCAGGTT CTCTCTGCTG CCACCATCGT TGCCAAACAC 
ACTTCGGCCT TGTGCAATGC CTGCCGGCTC GCCTCCTCCA AGACATCCAA CCCAGTTGCT 
AAGAGACAGT TCGTCCAGAG TGCCAAAGAG GTGGCCAACA GCACAGCCAA CCTCGTCAAG 
TCCATCAAGG CTCTCGACGG GGCCTTTAAC CAGGAAAACA GACAGAAGTG TAAGGAAGCC 
ACTGGGCCAC TCATTGAGGC TGTGGATAAC CTGACTGCCT TCGCTTCAAA CCCAGAGTTC 
GCAAGTGTAC CCGCTCAGAT CAGCCCAGAG GGTCTGGCTG CGATGGAGCC GATCACAGCA 
GCCGCTAAGG CAATGTTGGA AAGCTCGACA GGACTGATCC AGACGGCTCG CTCGCTCGCA 
GTCAACCCGA AAGATCCACC CAAGTGGTCT GTGCTTGCTG GACACTCCAG AACAGTGTCC 
GACTCCATCA AGAAACTCAT CACTAACATG AGGGAAAAGG CTCCGGGTCA GAGGGAGTGT 
GATGATGCCA TTGAGGTTCT TAGTAACTGC ATTCGTGAAG TGGACAAGGC CTCGCTGGCA 
GCCATCAGTC AGCAGCTCAC GCCTAGAGAC GACATTTCCC ATGAGGCTCT GCACGAGCAG 
ATGGCGGCGT CTGTGCATGA GATCAGTAAC CTGATTGAAC CAGTGGCCAT CGCAGCCCGT 
GCTGATGCCT CTCAGCTGGG ACACAAGGTG TCTCAGATGG TCAGTTACTT TGAACCGCTC 
ATCATGGCTG CCATCGGCAC AGCGTCTAAG ATCCTGAACA GCCAGCAGCA GATGAACGTG 
CTGGACCAAA CCAAGACTCT GGCTGAGTCT GCGCTACAGA TGCTCTATAC GGCCAAAGAG 
ACTGGAGGAA ACCACAAGGC GGCGCACACA CAAGAGGCTC TGGAGGAGTC TGTTCAGATG 
ATGAAGGAGG CGGTGGACGA TCTGGGTTCG ACTCTGGCAG AAGTGGCCAG CGCTGCAGGA 
GCAGTGGGAG GAATGGTCAA CTCCATCACT CAGTCCATCA ACAAGATGGA GGACGGACCT 
GCCTTCGAGC CTGAAGGAAC GTTTGTGGAT TATCAGACCA CCATGGTGAA GACAGCCAAA 
GCTATCGCTG TCACCGTTCA GGAGATGGTT ACCAAATCCA ACACCAACCC TGATGAGCTG 
GGCGGTTTGG CCAATCAGCT GACCACAGAG TTCGGTGACC TCGCATCAGA GGCCAAATGT 
GCAGCCATGA CTGCTGAAAA CGATGAGATC GGTTCACATA TCAAGAAGCA GGTGACTGAG 
TTGGGCTACA CCTGCACTGG TCTGGTCAGT AAAGCAGGAG CGCTACAGTG CAGTCCAAAC 
GACTCCTACA CTAAGAAGGA GCTCATTGAC AGTGCCAGGA AAGTCTCTGA GAAGGTCTCT 
CATGTGCTGG CAGCTCTTCA GGCAGGCAAT CGTGGCACTC AGGCCTGCAT TACTGCCGCG 
AGCGCTGTCT CTGGCATCAT TGCTGATTTA GACACCACCA TCATGTTTGC CACCGCGGGC 
ACTCTCAACC GAGAGAACGC AGAGACATTT GCAGACCACA GAGAGCACAT CTTAAAGACG 
GCTAAAGTTC TGGTGGAGGA CACGAAGCTG CTGGTTTCTG GAGCCGGAGC AAGTCAGGAG 
AAACTGGCTC AGGCTGCACA GTCCTCCGTC AACACCATCA CCAAACTGGC CGATGTGGTT 
AAATTGGGGG CAGCGAGTCT GGGCTCTGAA GACCCAGAGA CACAGGTGGT TCTGATCAAC 
GCTGTGAAGG ACGTGGCTAA AGCTCTGGGT GACCTGATCA GCGCCACCAA AGCAGCGGCC 
GGCAAACCTC ACGATGACCC CGCCATGCTG CAGCTCAAAA ACTCTGCTAA GGTGATGGTG 
ACCAATGTGA CGTCTCTACT TAAGACGGTG AAGGCGGTGG AGGATGAAGC CACTAAAGGC 
ACACGAGCAC TAGAGGCCAC CATAGAGCAC ATCAAACAAG AGCTGGCTGT TTTCAGCAGT 
GCAGATCCTC CTCCAAAGAC GGCCACTCCA GAGGAGTTCA TCCGCATGAC CAAAGGCATC 
ACATTGGCCA CAGCTAAAGC AGTGGCTGCT GGGAACTCCT GCCGTCAGGA GGACGTGATC 
GCCACTGCTA ACCTAAGCAG ACGAGCTGTC GCAGACATGC TGCGCTCCTG CAAGCAAGCC 
GCATACCACC CGGAGGTGAA GCGGGAGGTC CAGACTCGAG CGCTCCGCTT TGGCTCTGAG 
TGCTCCACAA GTTACCTGGG CCTGCTGGAG CACGTGTTAG TGCCGATCAT TCAGAAACCC 
TCGCATGACC TTAAACAGCA GCTGGCAAAC TACTCCAAAC GTGTGGCCAG TTCAGTGACC 
GAGCTCATCC AGGCTGCGGA GGCCATGAAA GGTACGGAGT GGGTGGATCC CGAAGACCCC 
ACCGTCATTG CAGAAAACGA GCTGCTGGGA GCCGCAGCTG CCATTGAAGC TGCCGCGAAG 
AAACTAGAGC AGCTCAAACC CCGAGCCAAA CCCAAGGAAG CTGATGAGAG TCTGAACTTT 
GAGGAACAGA TTCTAGAAGC GGCCAAATCC ATCGCTGCAG CCACCAGTGC TCTGGTCAAA 
GCTGCGTCTG CCGCTCAGAG AGAGCTGGTG GCTCAAGGGA AGGTTGGGGC AATTCCAGCA 
AACGCGGTGG ATGATGGACA GTGGTCTCAG GGTCTCATTT CAGCTGCTCG TATGGTAGCC 
GCAGCTACTA ACAACCTGTG CGAAGCAGCT AACTCTGCGG TTCAGGGTCA CGCCAGCGAG 
GAGAAACTCA TTTCTTCAGC CAAACAGGTG GCAGCATCCA CCGCACAGCT GCTGGTGGCC 
TGTAAGGTCA AGGCTGACCA GGACTCCCAG ACCATGAAGA GACTGCAGGC TGCTGGTAAT 
GCTGTCAAGA GGGCGTCTGA TAACCTGGTG AAGGCGGCAC AGAAGGCTGC GTTTGAGGCT 
CAAGATGATC AGGCTGTGAT GGTGAAGAGC AAGATGGTGG GCGGTATTGC TCAGATCATC 
GCTGCTCAGG AGGAGATGCT CAGGAAGGAG AAAGAGCTGG AAGAAGCCAG AAAGAAACTG 
GCAATGATCC GACAGCAGCA GTACAAGTTC CTGCCCTCAG AGCTGCGAGA GGACGGACAG 
GAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334843.1
CDS	203..7831
Translation	MVALSLKIGVGNVVKTMQFEPSTVVYDACRIIRERVPEAQLGQPSDYGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTLEYKKKQRPLKIRMLDGTVKTVMVDDSKIVSDMLMTICARIGITNYDEYSLVRDVGEEKKEETTGTLKRDKTLLRDDKKMEKLKQKLHTDDELNWLDHGRTLREQGVEESEMLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGYQCQIQFGDHNESKHKPGFLDLKEFLPKEYVKNKGEKRIFQAHKNCQNMTEIEAKVNYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKESVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQFNKMGKVETGSVALPAIIRSGAGGPESMKMGSMPQAKQHITSGQMHRGHMPPLTSAQQALTGTINSSMQAVQAVQASLDEFDALPPLGTDSASQAWRKNKMDESKHEIHSQVDAITAGTASMVNLTAGDPAETDYTAVGCAVTTISSNLTEMSKGVKLLAALMEDEGGNGQQLLGAAKNLACAVSDMLKTAQPSSTEPRQNLLQAAGNVGQASGELLQQIGESDADQHFQDMLMQLAKAVANAAAALVLKAKNVAQKSEDSAQQNKVIAAATQCALSTSQLVACTRVVAPTISSPVCQEQLIEAGKLVAKSVEGCVEASHGATGDEHLLKQVGAAATGVTQALNELLQHIRQYASGGQPIGRHGEATDRILDVTDNIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGESDLENSRKLLSAAKLLADATARMVEAAKGAAANPDSEEQQQKLREAAEGLRMATNAAAQNAIKKRLVNKLENAAKQAAAAATQTIAAAQHAASSNKNPAAQQQLVQSCKVVADQIPQLVQGLRSSQAQPDSPSAQLALIGASQNFLQPGAKMVTAAKATVPTISDQASAMQLSQCAKNLASALAELRTAAQKAQEACGPLEIDNALTMVRGLERDMQEAKASAAEGKLRPLPGETLEKCSQDLGSSTKAVSSAIAQLLSEATQGNENYTGMAARDVAQALRSLASAARGVAANTEDPLARNAMLDCTGDVMDKSASLIEETKRAIAKPGDPDSQQRLAQVAKAVSQALNRSVNCLPGQRDVDNAIRTVGEASKKLLSDKFPASGKNFQEAQANLNEAAAGLNQSANELVQASRGTTQDLAKASGKFGQDFNQFLQAGVDMAGQSQSKEDQTQVVTNLKTISMSSSKLLLAAKALSTDPNSPNLKNQLAAAARAVTDSINQLISMCTQQAPGQKECDNALRELETVGGMLENPTEAVNDLGYFDCIDSVMENSKVLGEAMAGISHNAKNSNLPEFGDSVSSASKALCGLTEAAAQGAYLVGVSDPNSHAGQKGLVDPSQFARAKQSIQMACQNLVDPACTQSQVLSAATIVAKHTSALCNACRLASSKTSNPVAKRQFVQSAKEVANSTANLVKSIKALDGAFNQENRQKCKEATGPLIEAVDNLTAFASNPEFASVPAQISPEGLAAMEPITAAAKAMLESSTGLIQTARSLAVNPKDPPKWSVLAGHSRTVSDSIKKLITNMREKAPGQRECDDAIEVLSNCIREVDKASLAAISQQLTPRDDISHEALHEQMAASVHEISNLIEPVAIAARADASQLGHKVSQMVSYFEPLIMAAIGTASKILNSQQQMNVLDQTKTLAESALQMLYTAKETGGNHKAAHTQEALEESVQMMKEAVDDLGSTLAEVASAAGAVGGMVNSITQSINKMEDGPAFEPEGTFVDYQTTMVKTAKAIAVTVQEMVTKSNTNPDELGGLANQLTTEFGDLASEAKCAAMTAENDEIGSHIKKQVTELGYTCTGLVSKAGALQCSPNDSYTKKELIDSARKVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNRENAETFADHREHILKTAKVLVEDTKLLVSGAGASQEKLAQAAQSSVNTITKLADVVKLGAASLGSEDPETQVVLINAVKDVAKALGDLISATKAAAGKPHDDPAMLQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATIEHIKQELAVFSSADPPPKTATPEEFIRMTKGITLATAKAVAAGNSCRQEDVIATANLSRRAVADMLRSCKQAAYHPEVKREVQTRALRFGSECSTSYLGLLEHVLVPIIQKPSHDLKQQLANYSKRVASSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATNNLCEAANSAVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSQTMKRLQAAGNAVKRASDNLVKAAQKAAFEAQDDQAVMVKSKMVGGIAQIIAAQEEMLRKEKELEEARKKLAMIRQQQYKFLPSELREDGQEQ

Target ORF information:

RefSeq Version	XM_021479168.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio talin 1 (tln1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479168.1

ORF Insert Sequence:

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGGACAT GTTGATGCAG TTGGCGAAGG CTGTGGCGAA TGCAGCAGCG 
GCTCTGGTGT TAAAGGCTAA AAACGTGGCT CAGAAAAGCG AAGATTCAGC TCAACAAAAC 
AAAGTGATCG CTGCTGCCAC CCAGTGCGCC CTCTCCACAT CACAGCTGGT GGCCTGCACA 
CGAGTGGTGG CTCCAACCAT CAGCTCACCC GTGTGTCAAG AGCAGCTCAT CGAGGCTGGA 
AAACTGGTGG CGAAGTCTGT GGAGGGCTGT GTGGAGGCGT CACACGGGGC GACCGGTGAC 
GAGCATCTGC TCAAACAGGT GGGCGCCGCT GCCACAGGAG TCACTCAGGC TCTTAACGAG 
CTGCTGCAGC ATATTAGACA GTACGCCAGC GGAGGACAGC CAATCGGACG GCACGGAGAG 
GCTACCGACC GCATCCTGGA TGTGACTGAT AACATCTTCA GCTCCATGGG AGATGCAGGG 
GAGATGGTGC GTCAGGCGCG GATCCTTGCT CAGGCCACCT CAGACCTGGT CAACGCGATC 
AAGGCGGACG CAGAGGGAGA ATCTGACCTG GAGAACTCTC GCAAACTCCT CTCTGCAGCC 
AAACTCCTGG CTGACGCTAC TGCCCGTATG GTGGAAGCTG CGAAGGGTGC AGCTGCTAAT 
CCAGACAGCG AGGAGCAGCA GCAGAAGTTG CGTGAAGCTG CAGAGGGACT GCGCATGGCC 
ACCAATGCTG CAGCACAGAA CGCCATTAAA AAGAGACTCG TCAACAAACT GGAGAATGCA 
GCGAAACAAG CCGCAGCAGC CGCCACTCAG ACCATAGCAG CCGCTCAACA TGCAGCTTCA 
TCCAACAAGA ACCCTGCGGC CCAGCAACAA CTCGTACAGA GCTGCAAGGT GGTGGCGGAT 
CAGATCCCAC AGCTGGTTCA GGGTCTGAGG AGCAGCCAGG CTCAACCTGA CAGCCCCAGC 
GCTCAACTCG CCCTCATCGG AGCTAGCCAG AACTTCCTGC AGCCCGGAGC CAAAATGGTG 
ACCGCAGCCA AAGCTACAGT TCCAACCATC AGTGACCAGG CGTCTGCCAT GCAGCTCAGT 
CAGTGCGCTA AAAACCTGGC CAGTGCTCTG GCTGAGCTCC GCACGGCCGC CCAGAAGGCT 
CAGGAGGCGT GTGGTCCGTT GGAGATTGAT AACGCTCTGA CTATGGTACG GGGGCTGGAG 
AGGGACATGC AGGAGGCCAA AGCTTCAGCT GCTGAGGGCA AACTCAGACC CCTGCCTGGA 
GAAACGCTGG AGAAGTGCTC TCAGGATCTG GGCTCCAGCA CTAAAGCCGT GAGCTCTGCC 
ATTGCTCAGC TCCTCAGTGA AGCAACTCAA GGCAATGAGA ACTACACAGG TATGGCTGCT 
CGTGATGTGG CTCAGGCTCT GCGCTCGCTG GCTTCAGCCG CTCGTGGAGT GGCAGCCAAC 
ACTGAAGATC CTCTAGCCCG TAACGCAATG CTGGACTGCA CCGGCGATGT CATGGACAAA 
TCCGCCAGCC TGATTGAAGA GACCAAGAGG GCCATAGCCA AACCAGGAGA CCCTGACAGT 
CAGCAAAGAC TGGCCCAGGT AGCCAAGGCT GTGTCTCAGG CACTGAACCG CTCTGTAAAC 
TGTCTGCCGG GGCAGAGGGA TGTGGATAAT GCCATCAGAA CTGTCGGGGA AGCTAGCAAG 
AAACTGCTGT CTGATAAATT TCCAGCGAGT GGGAAGAATT TCCAAGAGGC TCAGGCCAAT 
CTGAACGAGG CTGCAGCTGG ACTAAACCAG TCAGCCAATG AGCTGGTGCA GGCCTCGCGT 
GGCACCACCC AGGACCTGGC CAAAGCCTCC GGGAAGTTTG GACAAGACTT TAACCAATTC 
CTGCAGGCTG GAGTGGACAT GGCTGGACAA TCCCAGTCTA AAGAAGACCA AACTCAAGTG 
GTCACCAACC TGAAGACCAT CTCCATGTCC TCCAGCAAAC TCCTGCTGGC GGCTAAAGCT 
CTGTCCACAG ACCCCAACTC GCCAAATCTG AAGAACCAAC TCGCTGCTGC TGCTCGCGCG 
GTGACAGACA GCATTAATCA GCTGATCAGC ATGTGTACCC AGCAGGCTCC GGGACAGAAG 
GAGTGTGACA ACGCTCTCCG TGAGCTTGAG ACGGTTGGAG GAATGCTGGA GAACCCAACA 
GAAGCAGTCA ATGATTTGGG CTACTTTGAC TGCATTGACA GTGTGATGGA GAACTCAAAG 
GTTTTGGGTG AGGCCATGGC TGGCATTTCC CATAATGCCA AAAACAGTAA CTTGCCAGAG 
TTTGGAGATT CGGTCAGTTC AGCGTCTAAA GCGCTGTGTG GTCTCACTGA AGCAGCTGCT 
CAGGGGGCGT ATCTGGTTGG TGTGTCAGAC CCGAACAGCC ATGCTGGCCA GAAGGGTCTT 
GTGGACCCCT CACAATTTGC CAGAGCCAAA CAGTCCATCC AGATGGCCTG TCAGAACCTG 
GTGGATCCAG CCTGCACCCA GTCCCAGGTT CTCTCTGCTG CCACCATCGT TGCCAAACAC 
ACTTCGGCCT TGTGCAATGC CTGCCGGCTC GCCTCCTCCA AGACATCCAA CCCAGTTGCT 
AAGAGACAGT TCGTCCAGAG TGCCAAAGAG GTGGCCAACA GCACAGCCAA CCTCGTCAAG 
TCCATCAAGG CTCTCGACGG GGCCTTTAAC CAGGAAAACA GACAGAAGTG TAAGGAAGCC 
ACTGGGCCAC TCATTGAGGC TGTGGATAAC CTGACTGCCT TCGCTTCAAA CCCAGAGTTC 
GCAAGTGTAC CCGCTCAGAT CAGCCCAGAG GGTCTGGCTG CGATGGAGCC GATCACAGCA 
GCCGCTAAGG CAATGTTGGA AAGCTCGACA GGACTGATCC AGACGGCTCG CTCGCTCGCA 
GTCAACCCGA AAGATCCACC CAAGTGGTCT GTGCTTGCTG GACACTCCAG AACAGTGTCC 
GACTCCATCA AGAAACTCAT CACTAACATG AGGGAAAAGG CTCCGGGTCA GAGGGAGTGT 
GATGATGCCA TTGAGGTTCT TAGTAACTGC ATTCGTGAAG TGGACAAGGC CTCGCTGGCA 
GCCATCAGTC AGCAGCTCAC GCCTAGAGAC GACATTTCCC ATGAGGCTCT GCACGAGCAG 
ATGGCGGCGT CTGTGCATGA GATCAGTAAC CTGATTGAAC CAGTGGCCAT CGCAGCCCGT 
GCTGATGCCT CTCAGCTGGG ACACAAGGTG TCTCAGATGG TCAGTTACTT TGAACCGCTC 
ATCATGGCTG CCATCGGCAC AGCGTCTAAG ATCCTGAACA GCCAGCAGCA GATGAACGTG 
CTGGACCAAA CCAAGACTCT GGCTGAGTCT GCGCTACAGA TGCTCTATAC GGCCAAAGAG 
ACTGGAGGAA ACCACAAGGC GGCGCACACA CAAGAGGCTC TGGAGGAGTC TGTTCAGATG 
ATGAAGGAGG CGGTGGACGA TCTGGGTTCG ACTCTGGCAG AAGTGGCCAG CGCTGCAGGA 
GCAGTGGGAG GAATGGTCAA CTCCATCACT CAGTCCATCA ACAAGATGGA GGACGGACCT 
GCCTTCGAGC CTGAAGGAAC GTTTGTGGAT TATCAGACCA CCATGGTGAA GACAGCCAAA 
GCTATCGCTG TCACCGTTCA GGAGATGGTT ACCAAATCCA ACACCAACCC TGATGAGCTG 
GGCGGTTTGG CCAATCAGCT GACCACAGAG TTCGGTGACC TCGCATCAGA GGCCAAATGT 
GCAGCCATGA CTGCTGAAAA CGATGAGATC GGTTCACATA TCAAGAAGCA GGTGACTGAG 
TTGGGCTACA CCTGCACTGG TCTGGTCAGT AAAGCAGGAG CGCTACAGTG CAGTCCAAAC 
GACTCCTACA CTAAGAAGGA GCTCATTGAC AGTGCCAGGA AAGTCTCTGA GAAGGTCTCT 
CATGTGCTGG CAGCTCTTCA GGCAGGCAAT CGTGGCACTC AGGCCTGCAT TACTGCCGCG 
AGCGCTGTCT CTGGCATCAT TGCTGATTTA GACACCACCA TCATGTTTGC CACCGCGGGC 
ACTCTCAACC GAGAGAACGC AGAGACATTT GCAGACCACA GAGAGCACAT CTTAAAGACG 
GCTAAAGTTC TGGTGGAGGA CACGAAGCTG CTGGTTTCTG GAGCCGGAGC AAGTCAGGAG 
AAACTGGCTC AGGCTGCACA GTCCTCCGTC AACACCATCA CCAAACTGGC CGATGTGGTT 
AAATTGGGGG CAGCGAGTCT GGGCTCTGAA GACCCAGAGA CACAGGTGGT TCTGATCAAC 
GCTGTGAAGG ACGTGGCTAA AGCTCTGGGT GACCTGATCA GCGCCACCAA AGCAGCGGCC 
GGCAAACCTC ACGATGACCC CGCCATGCTG CAGCTCAAAA ACTCTGCTAA GGTGATGGTG 
ACCAATGTGA CGTCTCTACT TAAGACGGTG AAGGCGGTGG AGGATGAAGC CACTAAAGGC 
ACACGAGCAC TAGAGGCCAC CATAGAGCAC ATCAAACAAG AGCTGGCTGT TTTCAGCAGT 
GCAGATCCTC CTCCAAAGAC GGCCACTCCA GAGGAGTTCA TCCGCATGAC CAAAGGCATC 
ACATTGGCCA CAGCTAAAGC AGTGGCTGCT GGGAACTCCT GCCGTCAGGA GGACGTGATC 
GCCACTGCTA ACCTAAGCAG ACGAGCTGTC GCAGACATGC TGCGCTCCTG CAAGCAAGCC 
GCATACCACC CGGAGGTGAA GCGGGAGGTC CAGACTCGAG CGCTCCGCTT TGGCTCTGAG 
TGCTCCACAA GTTACCTGGG CCTGCTGGAG CACGTGTTAG TGCCGATCAT TCAGAAACCC 
TCGCATGACC TTAAACAGCA GCTGGCAAAC TACTCCAAAC GTGTGGCCAG TTCAGTGACC 
GAGCTCATCC AGGCTGCGGA GGCCATGAAA GGTACGGAGT GGGTGGATCC CGAAGACCCC 
ACCGTCATTG CAGAAAACGA GCTGCTGGGA GCCGCAGCTG CCATTGAAGC TGCCGCGAAG 
AAACTAGAGC AGCTCAAACC CCGAGCCAAA CCCAAGGAAG CTGATGAGAG TCTGAACTTT 
GAGGAACAGA TTCTAGAAGC GGCCAAATCC ATCGCTGCAG CCACCAGTGC TCTGGTCAAA 
GCTGCGTCTG CCGCTCAGAG AGAGCTGGTG GCTCAAGGGA AGGTTGGGGC AATTCCAGCA 
AACGCGGTGG ATGATGGACA GTGGTCTCAG GGTCTCATTT CAGCTGCTCG TATGGTAGCC 
GCAGCTACTA ACAACCTGTG CGAAGCAGCT AACTCTGCGG TTCAGGGTCA CGCCAGCGAG 
GAGAAACTCA TTTCTTCAGC CAAACAGGTG GCAGCATCCA CCGCACAGCT GCTGGTGGCC 
TGTAAGGTCA AGGCTGACCA GGACTCCCAG ACCATGAAGA GACTGCAGGC TGCTGGTAAT 
GCTGTCAAGA GGGCGTCTGA TAACCTGGTG AAGGCGGCAC AGAAGGCTGC GTTTGAGGCT 
CAAGATGATC AGGCTGTGAT GGTGAAGAGC AAGATGGTGG GCGGTATTGC TCAGATCATC 
GCTGCTCAGG AGGAGATGCT CAGGAAGGAG AAAGAGCTGG AAGAAGCCAG AAAGAAACTG 
GCAATGATCC GACAGCAGCA GTACAAGTTC CTGCCCTCAG AGCTGCGAGA GGACGGACAG 
GAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tln1 ( NM_001009560.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001009560.1 Danio rerio talin 1 (tln1), mRNA.

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CloneID	ODa32586
Clone ID Related Accession (Same CDS sequence)	NM_001009560.1
Accession Version	NM_001009560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-07
Organism	Danio rerio(zebrafish)
Product	talin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY781336.1. ##Evidence-Data-START## Transcript exon combination :: AY781336.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA3505388 [ECO:0000350] ##Evidence-Data-END##

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTGGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGTTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGCCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGGACTTTGG AGACTATCAG GATGGTTACT ACTCAGTTCA GACCACTGAA 
GGAGAACAGA TTGCACAACT CATTGCTGGA TACATTGACA TCATTCTCAA AAAGAAAAAG 
AGCAAAGATC ACTTCGGCCT GGAGGGAGAT GAAGAGTCAA CCATGTTGGA GGACTCCGTC 
TCTCCGAAAA AGTCAACAGT TCTTCAGCAG CAGTTTAATA AGATGGGGAA GGTGGAGACG 
GGTTCTGTCG CTCTACCGGC CATCATCCGA TCTGGTGCTG GAGGTCCAGA GAGCATGAAG 
ATGGGCTCAA TGCCACAGGC CAAACAGCAC ATCACCAGCG GTCAGATGCA CAGAGGACAT 
ATGCCCCCTC TGACTTCAGC TCAGCAGGCT TTAACTGGCA CCATTAACTC CAGCATGCAG 
GCAGTACAGG CGGTTCAGGC TTCTCTTGAT GAGTTTGATG CCCTGCCTCC ACTGGGGACT 
GATTCGGCAT CTCAAGCCTG GAGGAAGAAC AAAATGGATG AGTCCAAACA CGAGATTCAC 
TCTCAGGTGG ATGCCATCAC TGCGGGAACT GCTTCCATGG TCAATCTGAC TGCAGGTGAC 
CCGGCCGAGA CAGATTACAC TGCTGTGGGC TGTGCCGTCA CCACCATCTC CTCCAACCTG 
ACGGAGATGT CCAAGGGTGT CAAACTGCTG GCGGCGCTGA TGGAGGACGA GGGCGGAAAT 
GGCCAGCAGC TTCTGGGTGC TGCAAAGAAC CTGGCGTGTG CCGTGTCTGA TATGCTCAAG 
ACTGCTCAGC CGTCCAGCAC TGAGCCACGA CAGAACCTCC TGCAAGCAGC GGGTAATGTG 
GGTCAGGCCA GCGGTGAACT CCTGCAGCAG ATCGGGGAGT CTGATGCGGA TCAGCACTTC 
CAGGACATGT TGATGCAGTT GGCGAAGGCT GTGGCGAATG CAGCAGCGGC TCTGGTGTTA 
AAGGCTAAAA ACGTGGCTCA GAAAAGCGAA GATTCAGCTC AACAAAACAA AGTGATCGCT 
GCTGCCACCC AGTGCGCCCT CTCCACATCA CAGCTGGTGG CCTGCACACG AGTGGTGGTG 
GCTCCAACCA TCAGCTCACC CGTGTGTCAA GAGCAGCTCA TCGAGGCTGG AAAACTGGTG 
GCGAAGTCTG TGGAGGGCTG TGTGGAGGCG TCACACGGGG CGACCGGTGA CGAGCATCTG 
CTCAAACAGG TGGGCGCCGC TGCCACAGGA GTCACTCAGG CTCTTAACGA GCTGCTGCAG 
CATATTAGAC AGTACGCCAG CGGAGGACAG CCAATCGGAC GGCACGGAGA GGCTACCGAC 
CGCATCCTGG ATGTGACTGA TAACATCTTC AGCTCCATGG GAGATGCAGG GGAGATGGTG 
CGTCAGGCGC GGATCCTTGC TCAGGCCACC TCAGACCTGG TCAACGCGAT CAAGGCGGAC 
GCAGAGGGAG AATCTGACCT GGAGAACTCT CGCAAACTCC TCTCTGCAGC CAAACTCCTG 
GCTGACGCTA CTGCCCGTAT GGTGGAAGCT GCGAAGGGTG CAGCTGCTAA TCCAGACAGC 
GAGGAGCAGC AGCAGAAGTT GCGTGAAGCT GCAGAGGGAC TGCGCATGGC CACCAATGCT 
GCAGCACAGA ACGCCATTAA AAAGAGACTC GTCAACAAAC TGGAGAATGC AGCGAAACAA 
GCCGCAGCAG CCGCCACTCA GACCATAGCA GCCGCTCAAC ATGCAGCTTC ATCCAACAAG 
AACCCTGCGG CCCAGCAACA ACTCGTACAG AGCTGCAAGG TGGTGGCGGA TCAGATCCCA 
CAGCTGGTTC AGGGTCTGAG GAGCAGCCAG GCTCAACCTG ACAGCCCCAG CGCTCAACTC 
GCCCTCATCG GAGCTAGCCA GAACTTCCTG CAGCCCGGAG CCAAAATGGT GACCGCAGCC 
AAAGCTACAG TTCCAACCAT CAGTGACCAG GCGTCTGCCA TGCAGCTCAG TCAGTGCGCT 
AAAAACCTGG CCAGTGCTCT GGCTGAGCTC CGCACGGCCG CCCAGAAGGC TCAGGAGGCG 
TGTGGTCCGT TGGAGATTGA TAACGCTCTG ACTATGGTAC GGGGGCTGGA GAGGGACATG 
CAGGAGGCCA AAGCTTCAGC TGCTGAGGGC AAACTCAGAC CCCTGCCTGG AGAAACGCTG 
GAGAAGTGCT CTCAGGATCT GGGCTCCAGC ACTAAAGCCG TGAGCTCTGC CATTGCTCAG 
CTCCTCAGTG AAGCAACTCA AGGCAATGAG AACTACACAG GTATGGCTGC TCGTGATGTG 
GCTCAGGCTC TGCGCTCGCT GGCTTCAGCC GCTCGTGGAG TGGCAGCCAA CACTGAAGAT 
CCTCTAGCCC GTAACGCAAT GCTGGACTGC ACCGGCGATG TCATGGACAA ATCCGCCAGC 
CTGATTGAAG AGACCAAGAG GGCCATAGCC AAACCAGGAG ACCCTGACAG TCAGCAAAGA 
CTGGCCCAGG TAGCCAAGGC TGTGTCTCAG GCACTGAACC GCTCTGTAAA CTGTCTGCCG 
GGGCAGAGGG ATGTGGATAA TGCCATCAGA ACTGTCGGGG AAGCTAGCAA GAAACTGCTG 
TCTGATAAAT TTCCAGCGAG TGGGAAGAAT TTCCAAGAGG CTCAGGCCAA TCTGAACGAG 
GCTGCAGCTG GACTAAACCA GTCAGCCAAT GAGCTGGTGC AGGCCTCGCG TGGCACCACC 
CAGGACCTGG CCAAAGCCTC CGGGAAGTTT GGACAAGACT TTAACCAATT CCTGCAGGCT 
GGAGTGGACA TGGCTGGACA ATCCCAGTCT AAAGAAGACC AAACTCAAGT GGTCACCAAC 
CTGAAGACCA TCTCCATGTC CTCCAGCAAA CTCCTGCTGG CGGCTAAAGC TCTGTCCACA 
GACCCCAACT CGCCAAATCT GAAGAACCAA CTCGCTGCTG CTGCTCGCGC GGTGACAGAC 
AGCATTAATC AGCTGATCAG CATGTGTACC CAGCAGGCTC CGGGACAGAA GGAGTGTGAC 
AACGCTCTCC GTGAGCTTGA GACGGTTGGA GGAATGCTGG AGAACCCAAC AGAAGCAGTC 
AATGATTTGG GCTACTTTGA CTGCATTGAC AGTGTGATGG AGAACTCAAA GGTTTTGGGT 
GAGGCCATGG CTGGCATTTC CCATAATGCC AAAAACAGTA ACTTGCCAGA GTTTGGAGAT 
TCGGTCAGTT CAGCGTCTAA AGCGCTGTGT GGTCTCACTG AAGCAGCTGC TCAGGGGGCG 
TATCTGGTTG GTGTGTCAGA CCCGAACAGC CATGCTGGCC AGAAGGGTCT TGTGGACCCC 
TCACAATTTG CCAGAGCCAA ACAGTCCATC CAGATGGCCT GTCAGAACCT GGTGGATCCA 
GCCTGCACCC AGTCCCAGGT TCTCTCTGCT GCCACCATCG TTGCCAAACA CACTTCGGCC 
TTGTGCAATG CCTGCCGGCT CGCCTCCTCC AAGACATCCA ACCCAGTTGC TAAGAGACAG 
TTCGTCCAGA GTGCCAAAGA GGTGGCCAAC AGCACAGCCA ACCTCGTCAA GTCCATCAAG 
GCTCTCGACG GGGCCTTTAA CCAGGAAAAC AGACAGAAGT GTAAGGAAGC CACTGGGCCA 
CTCATTGAGG CTGTGGATAA CCTGACTGCC TTCGCTTCAA ACCCAGAGTT CGCAAGTGTA 
CCCGCTCAGA TCAGCCCAGA GGGTCTGGCT GCGATGGAGC CGATCACAGC AGCCGCTAAG 
GCAATGTTGG AAAGCTCGAC AGGACTGATC CAGACGGCTC GCTCGCTCGC AGTCAACCCG 
AAAGATCCAC CCAAGTGGTC TGTGCTTGCT GGACACTCCA GAACAGTGTC CGACTCCATC 
AAGAAACTCA TCACTAACAT GAGGGAAAAG GCTCCGGGTC AGAGGGAGTG TGATGATGCC 
ATTGAGGTTC TTAGTAACTG CATTCGTGAA GTGGACAAGG CCTCGCTGGC AGCCATCAGT 
CAGCAGCTCA CGCCTAGAGA CGACATTTCC CATGAGGCTC TGCACGAGCA GATGGCGGCG 
TCTGTGCATG AGATCAGTAA CCTGATTGAA CCAGTGGCCA TCGCAGCCCG TGCTGATGCC 
TCTCAGCTGG GACACAAGGT GTCTCAGATG GTCAGTTACT TTGAACCGCT CATCATGGCT 
GCCATCGGCA CAGCGTCTAA GATCCTGAAC AGCCAGCAGC AGATGAACGT GCTGGACCAA 
ACCAAGACTC TGGCTGAGTC TGCGCTACAG ATGCTCTATA CGGCCAAAGA GACTGGAGGA 
AACCACAAGG CGGCGCACAC ACAAGAGGCT CTGGAGGAGT CTGTTCAGAT GATGAAGGAG 
GCGGTGGACG ATCTGGGTTC GACTCTGGCA GAAGTGGCCA GCGCTGCAGG AGCAGTGGGA 
GGAATGGTCA ACTCCATCAC TCAGTCCATC AACAAGATGG AGGACGGACC TGCCTTCGAG 
CCTGAAGGAA CGTTTGTGGA TTATCAGACC ACCATGGTGA AGACAGCCAA AGCTATCGCT 
GTCACCGTTC AGGAGATGGT TACCAAATCC AACACCAACC CTGATGAGCT GGGCGGTTTG 
GCCAATCAGC TGACCACAGA GTTCGGTGAC CTCGCATCAG AGGCCAAATG TGCAGCCATG 
ACTGCTGAAA ACGATGAGAT CGGTTCACAT ATCAAGAAGC AGGTGACTGA GTTGGGCTAC 
ACCTGCACTG GTCTGGTCAG TAAAGCAGGA GCGCTACAGT GCAGTCCAAA CGACTCCTAC 
ACTAAGAAGG AGCTCATTGA CAGTGCCAGG AAAGTCTCTG AGAAGGTCTC TCATGTGCTG 
GCAGCTCTTC AGGCAGGCAA TCGTGGCACT CAGGCCTGCA TTACTGCCGC GAGCGCTGTC 
TCTGGCATCA TTGCTGATTT AGACACCACC ATCATGTTTG CCACCGCGGG CACTCTCAAC 
CGAGAGAACG CAGAGACATT TGCAGACCAC AGAGAGCACA TCTTAAAGAC GGCTAAAGTT 
CTGGTGGAGG ACACGAAGCT GCTGGTTTCT GGAGCCGGAG CAAGTCAGGA GAAACTGGCT 
CAGGCTGCAC AGTCCTCCGT CAACACCATC ACCAAACTGG CCGATGTGGT TAAATTGGGG 
GCAGCGAGTC TGGGCTCTGA AGACCCAGAG ACACAGGTGG TTCTGATCAA CGCTGTGAAG 
GACGTGGCTA AAGCTCTGGG TGACCTGATC AGCGCCACCA AAGCAGCGGC CGGCAAACCT 
CACGATGACC CCGCCATGCT GCAGCTCAAA AACTCTGCTA AGGTGATGGT GACCAATGTG 
ACGTCTCTAC TTAAAACCGT GAAAGCCGTG GAGGATGAAG CCACTAAAGG CACACGAGCA 
CTAGAGGCCA CCATAGAGCA CATCAAACAA GAGCTGGCTG TTTTCAGCAG TGCAGATCCT 
CCTCCAAAGA CGGCCACTCC AGAGGAGTTC ATCCGCATGA CCAAAGGCAT CACATTGGCC 
ACAGCTAAAG CAGTGGCTGC TGGGAACTCC TGCCGTCAGG AGGACGTGAT CGCCACTGCT 
AACCTAAGCA GACGAGCTGT CGCAGACATG CTGCGCTCCT GCAAGCAAGC CGCATACCAC 
CCAGAGGTGA AGCGGGAGGT CCAGACTCGA GCGCTCCGCT TTGGCTCTGA GTGCTCCACA 
AGTTACCTGG GCCTGCTGGA GCACGTGTTA GTGATCATTC AGAAACCCTC GCATGACCTT 
AAACAGCAGC TGGCAAACTA CTCCAAACGT GTGGCCAGTT CAGTGACCGA GCTCATCCAG 
GCTGCGGAGG CCATGAAAGG TACGGAGTGG GTGGATCCCG AAGACCCCAC CGTCATTGCA 
GAAAACGAGC TGCTGGGAGC CGCAGCTGCC ATTGAAGCTG CCGCGAAGAA ACTAGAGCAG 
CTCAAACCCC GAGCCAAACC CAAGGAAGCT GATGAGAGTC TGAACTTTGA GGAACAGATT 
CTAGAAGCGG CCAAATCCAT CGCTGCAGCC ACCAGTGCTC TGGTCAAAGC TGCGTCTGCC 
GCTCAGAGAG AGCTGGTGGC TCAAGGGAAG GTTGGGGCAA TTCCAGCAAA TGCGGTGGAT 
GATGGACAGT GGTCTCAGGG TCTCATTTCA GCTGCTCGTA TGGTAGCTGC AGCTACTAAC 
AACCTGTGCG AAGCAGCTAA CTCTGCGGTT CAGGGTCACG CCAGCGAGGA GAAACTCATT 
TCTTCAGCCA AACAGGTGGC AGCATCCACC GCACAGCTGC TGGTGGCCTG TAAGGTCAAG 
GCTGACCAGG ACTCCCAGAC CATGAAGAGA CTGCAGGCTG CTGGTAATGC TGTCAAGAGG 
GCGTCTGATA ACCTGGTGAA GGCGGCACAG AAGGCTGCGT TTGAGGCTCA AGATGATCAG 
GCTGTGATGG TGAAGAGCAA GATGGTGGGC GGTATTGCTC AGATCATCGC TGCTCAGGAG 
GAGATGCTCA GGAAGGAGAA AGAGCTGGAA GAAGCCAGAA AGAAACTGGC AATGATCCGA 
CAGCAGCAGT ACAAGTTCCT GCCCTCAGAG CTGCGAGAGG ACGGACAGGA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009560.1
CDS	142..7758
Translation	MVALSLKIGVGNVVKTMQFEPSTVVYDACRIIRERVPEAQLGQPSDYGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTLEYKKKQRPLKIRMLDGTVKTVMVDDSKIVSDMLMTICARIGITNYDEYSLVRDVGEEKKEETTGTLKRDKTLLRDDKKMEKLKQKLHTDDELNWLDHGRTLREQGVEESEMLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGYQCQIQFGDHNESKHKPGFLDLKEFLPKEYVKNKGEKRIFQAHKNCQNMTEIEAKVNYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKESVMRVDEKTKEVIQEWSLTNIKRWAASPKDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQFNKMGKVETGSVALPAIIRSGAGGPESMKMGSMPQAKQHITSGQMHRGHMPPLTSAQQALTGTINSSMQAVQAVQASLDEFDALPPLGTDSASQAWRKNKMDESKHEIHSQVDAITAGTASMVNLTAGDPAETDYTAVGCAVTTISSNLTEMSKGVKLLAALMEDEGGNGQQLLGAAKNLACAVSDMLKTAQPSSTEPRQNLLQAAGNVGQASGELLQQIGESDADQHFQDMLMQLAKAVANAAAALVLKAKNVAQKSEDSAQQNKVIAAATQCALSTSQLVACTRVVVAPTISSPVCQEQLIEAGKLVAKSVEGCVEASHGATGDEHLLKQVGAAATGVTQALNELLQHIRQYASGGQPIGRHGEATDRILDVTDNIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGESDLENSRKLLSAAKLLADATARMVEAAKGAAANPDSEEQQQKLREAAEGLRMATNAAAQNAIKKRLVNKLENAAKQAAAAATQTIAAAQHAASSNKNPAAQQQLVQSCKVVADQIPQLVQGLRSSQAQPDSPSAQLALIGASQNFLQPGAKMVTAAKATVPTISDQASAMQLSQCAKNLASALAELRTAAQKAQEACGPLEIDNALTMVRGLERDMQEAKASAAEGKLRPLPGETLEKCSQDLGSSTKAVSSAIAQLLSEATQGNENYTGMAARDVAQALRSLASAARGVAANTEDPLARNAMLDCTGDVMDKSASLIEETKRAIAKPGDPDSQQRLAQVAKAVSQALNRSVNCLPGQRDVDNAIRTVGEASKKLLSDKFPASGKNFQEAQANLNEAAAGLNQSANELVQASRGTTQDLAKASGKFGQDFNQFLQAGVDMAGQSQSKEDQTQVVTNLKTISMSSSKLLLAAKALSTDPNSPNLKNQLAAAARAVTDSINQLISMCTQQAPGQKECDNALRELETVGGMLENPTEAVNDLGYFDCIDSVMENSKVLGEAMAGISHNAKNSNLPEFGDSVSSASKALCGLTEAAAQGAYLVGVSDPNSHAGQKGLVDPSQFARAKQSIQMACQNLVDPACTQSQVLSAATIVAKHTSALCNACRLASSKTSNPVAKRQFVQSAKEVANSTANLVKSIKALDGAFNQENRQKCKEATGPLIEAVDNLTAFASNPEFASVPAQISPEGLAAMEPITAAAKAMLESSTGLIQTARSLAVNPKDPPKWSVLAGHSRTVSDSIKKLITNMREKAPGQRECDDAIEVLSNCIREVDKASLAAISQQLTPRDDISHEALHEQMAASVHEISNLIEPVAIAARADASQLGHKVSQMVSYFEPLIMAAIGTASKILNSQQQMNVLDQTKTLAESALQMLYTAKETGGNHKAAHTQEALEESVQMMKEAVDDLGSTLAEVASAAGAVGGMVNSITQSINKMEDGPAFEPEGTFVDYQTTMVKTAKAIAVTVQEMVTKSNTNPDELGGLANQLTTEFGDLASEAKCAAMTAENDEIGSHIKKQVTELGYTCTGLVSKAGALQCSPNDSYTKKELIDSARKVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNRENAETFADHREHILKTAKVLVEDTKLLVSGAGASQEKLAQAAQSSVNTITKLADVVKLGAASLGSEDPETQVVLINAVKDVAKALGDLISATKAAAGKPHDDPAMLQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATIEHIKQELAVFSSADPPPKTATPEEFIRMTKGITLATAKAVAAGNSCRQEDVIATANLSRRAVADMLRSCKQAAYHPEVKREVQTRALRFGSECSTSYLGLLEHVLVIIQKPSHDLKQQLANYSKRVASSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATNNLCEAANSAVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSQTMKRLQAAGNAVKRASDNLVKAAQKAAFEAQDDQAVMVKSKMVGGIAQIIAAQEEMLRKEKELEEARKKLAMIRQQQYKFLPSELREDGQEQ

Target ORF information:

RefSeq Version	NM_001009560.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio talin 1 (tln1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009560.1

ORF Insert Sequence:

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTGGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGTTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGCCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGGACTTTGG AGACTATCAG GATGGTTACT ACTCAGTTCA GACCACTGAA 
GGAGAACAGA TTGCACAACT CATTGCTGGA TACATTGACA TCATTCTCAA AAAGAAAAAG 
AGCAAAGATC ACTTCGGCCT GGAGGGAGAT GAAGAGTCAA CCATGTTGGA GGACTCCGTC 
TCTCCGAAAA AGTCAACAGT TCTTCAGCAG CAGTTTAATA AGATGGGGAA GGTGGAGACG 
GGTTCTGTCG CTCTACCGGC CATCATCCGA TCTGGTGCTG GAGGTCCAGA GAGCATGAAG 
ATGGGCTCAA TGCCACAGGC CAAACAGCAC ATCACCAGCG GTCAGATGCA CAGAGGACAT 
ATGCCCCCTC TGACTTCAGC TCAGCAGGCT TTAACTGGCA CCATTAACTC CAGCATGCAG 
GCAGTACAGG CGGTTCAGGC TTCTCTTGAT GAGTTTGATG CCCTGCCTCC ACTGGGGACT 
GATTCGGCAT CTCAAGCCTG GAGGAAGAAC AAAATGGATG AGTCCAAACA CGAGATTCAC 
TCTCAGGTGG ATGCCATCAC TGCGGGAACT GCTTCCATGG TCAATCTGAC TGCAGGTGAC 
CCGGCCGAGA CAGATTACAC TGCTGTGGGC TGTGCCGTCA CCACCATCTC CTCCAACCTG 
ACGGAGATGT CCAAGGGTGT CAAACTGCTG GCGGCGCTGA TGGAGGACGA GGGCGGAAAT 
GGCCAGCAGC TTCTGGGTGC TGCAAAGAAC CTGGCGTGTG CCGTGTCTGA TATGCTCAAG 
ACTGCTCAGC CGTCCAGCAC TGAGCCACGA CAGAACCTCC TGCAAGCAGC GGGTAATGTG 
GGTCAGGCCA GCGGTGAACT CCTGCAGCAG ATCGGGGAGT CTGATGCGGA TCAGCACTTC 
CAGGACATGT TGATGCAGTT GGCGAAGGCT GTGGCGAATG CAGCAGCGGC TCTGGTGTTA 
AAGGCTAAAA ACGTGGCTCA GAAAAGCGAA GATTCAGCTC AACAAAACAA AGTGATCGCT 
GCTGCCACCC AGTGCGCCCT CTCCACATCA CAGCTGGTGG CCTGCACACG AGTGGTGGTG 
GCTCCAACCA TCAGCTCACC CGTGTGTCAA GAGCAGCTCA TCGAGGCTGG AAAACTGGTG 
GCGAAGTCTG TGGAGGGCTG TGTGGAGGCG TCACACGGGG CGACCGGTGA CGAGCATCTG 
CTCAAACAGG TGGGCGCCGC TGCCACAGGA GTCACTCAGG CTCTTAACGA GCTGCTGCAG 
CATATTAGAC AGTACGCCAG CGGAGGACAG CCAATCGGAC GGCACGGAGA GGCTACCGAC 
CGCATCCTGG ATGTGACTGA TAACATCTTC AGCTCCATGG GAGATGCAGG GGAGATGGTG 
CGTCAGGCGC GGATCCTTGC TCAGGCCACC TCAGACCTGG TCAACGCGAT CAAGGCGGAC 
GCAGAGGGAG AATCTGACCT GGAGAACTCT CGCAAACTCC TCTCTGCAGC CAAACTCCTG 
GCTGACGCTA CTGCCCGTAT GGTGGAAGCT GCGAAGGGTG CAGCTGCTAA TCCAGACAGC 
GAGGAGCAGC AGCAGAAGTT GCGTGAAGCT GCAGAGGGAC TGCGCATGGC CACCAATGCT 
GCAGCACAGA ACGCCATTAA AAAGAGACTC GTCAACAAAC TGGAGAATGC AGCGAAACAA 
GCCGCAGCAG CCGCCACTCA GACCATAGCA GCCGCTCAAC ATGCAGCTTC ATCCAACAAG 
AACCCTGCGG CCCAGCAACA ACTCGTACAG AGCTGCAAGG TGGTGGCGGA TCAGATCCCA 
CAGCTGGTTC AGGGTCTGAG GAGCAGCCAG GCTCAACCTG ACAGCCCCAG CGCTCAACTC 
GCCCTCATCG GAGCTAGCCA GAACTTCCTG CAGCCCGGAG CCAAAATGGT GACCGCAGCC 
AAAGCTACAG TTCCAACCAT CAGTGACCAG GCGTCTGCCA TGCAGCTCAG TCAGTGCGCT 
AAAAACCTGG CCAGTGCTCT GGCTGAGCTC CGCACGGCCG CCCAGAAGGC TCAGGAGGCG 
TGTGGTCCGT TGGAGATTGA TAACGCTCTG ACTATGGTAC GGGGGCTGGA GAGGGACATG 
CAGGAGGCCA AAGCTTCAGC TGCTGAGGGC AAACTCAGAC CCCTGCCTGG AGAAACGCTG 
GAGAAGTGCT CTCAGGATCT GGGCTCCAGC ACTAAAGCCG TGAGCTCTGC CATTGCTCAG 
CTCCTCAGTG AAGCAACTCA AGGCAATGAG AACTACACAG GTATGGCTGC TCGTGATGTG 
GCTCAGGCTC TGCGCTCGCT GGCTTCAGCC GCTCGTGGAG TGGCAGCCAA CACTGAAGAT 
CCTCTAGCCC GTAACGCAAT GCTGGACTGC ACCGGCGATG TCATGGACAA ATCCGCCAGC 
CTGATTGAAG AGACCAAGAG GGCCATAGCC AAACCAGGAG ACCCTGACAG TCAGCAAAGA 
CTGGCCCAGG TAGCCAAGGC TGTGTCTCAG GCACTGAACC GCTCTGTAAA CTGTCTGCCG 
GGGCAGAGGG ATGTGGATAA TGCCATCAGA ACTGTCGGGG AAGCTAGCAA GAAACTGCTG 
TCTGATAAAT TTCCAGCGAG TGGGAAGAAT TTCCAAGAGG CTCAGGCCAA TCTGAACGAG 
GCTGCAGCTG GACTAAACCA GTCAGCCAAT GAGCTGGTGC AGGCCTCGCG TGGCACCACC 
CAGGACCTGG CCAAAGCCTC CGGGAAGTTT GGACAAGACT TTAACCAATT CCTGCAGGCT 
GGAGTGGACA TGGCTGGACA ATCCCAGTCT AAAGAAGACC AAACTCAAGT GGTCACCAAC 
CTGAAGACCA TCTCCATGTC CTCCAGCAAA CTCCTGCTGG CGGCTAAAGC TCTGTCCACA 
GACCCCAACT CGCCAAATCT GAAGAACCAA CTCGCTGCTG CTGCTCGCGC GGTGACAGAC 
AGCATTAATC AGCTGATCAG CATGTGTACC CAGCAGGCTC CGGGACAGAA GGAGTGTGAC 
AACGCTCTCC GTGAGCTTGA GACGGTTGGA GGAATGCTGG AGAACCCAAC AGAAGCAGTC 
AATGATTTGG GCTACTTTGA CTGCATTGAC AGTGTGATGG AGAACTCAAA GGTTTTGGGT 
GAGGCCATGG CTGGCATTTC CCATAATGCC AAAAACAGTA ACTTGCCAGA GTTTGGAGAT 
TCGGTCAGTT CAGCGTCTAA AGCGCTGTGT GGTCTCACTG AAGCAGCTGC TCAGGGGGCG 
TATCTGGTTG GTGTGTCAGA CCCGAACAGC CATGCTGGCC AGAAGGGTCT TGTGGACCCC 
TCACAATTTG CCAGAGCCAA ACAGTCCATC CAGATGGCCT GTCAGAACCT GGTGGATCCA 
GCCTGCACCC AGTCCCAGGT TCTCTCTGCT GCCACCATCG TTGCCAAACA CACTTCGGCC 
TTGTGCAATG CCTGCCGGCT CGCCTCCTCC AAGACATCCA ACCCAGTTGC TAAGAGACAG 
TTCGTCCAGA GTGCCAAAGA GGTGGCCAAC AGCACAGCCA ACCTCGTCAA GTCCATCAAG 
GCTCTCGACG GGGCCTTTAA CCAGGAAAAC AGACAGAAGT GTAAGGAAGC CACTGGGCCA 
CTCATTGAGG CTGTGGATAA CCTGACTGCC TTCGCTTCAA ACCCAGAGTT CGCAAGTGTA 
CCCGCTCAGA TCAGCCCAGA GGGTCTGGCT GCGATGGAGC CGATCACAGC AGCCGCTAAG 
GCAATGTTGG AAAGCTCGAC AGGACTGATC CAGACGGCTC GCTCGCTCGC AGTCAACCCG 
AAAGATCCAC CCAAGTGGTC TGTGCTTGCT GGACACTCCA GAACAGTGTC CGACTCCATC 
AAGAAACTCA TCACTAACAT GAGGGAAAAG GCTCCGGGTC AGAGGGAGTG TGATGATGCC 
ATTGAGGTTC TTAGTAACTG CATTCGTGAA GTGGACAAGG CCTCGCTGGC AGCCATCAGT 
CAGCAGCTCA CGCCTAGAGA CGACATTTCC CATGAGGCTC TGCACGAGCA GATGGCGGCG 
TCTGTGCATG AGATCAGTAA CCTGATTGAA CCAGTGGCCA TCGCAGCCCG TGCTGATGCC 
TCTCAGCTGG GACACAAGGT GTCTCAGATG GTCAGTTACT TTGAACCGCT CATCATGGCT 
GCCATCGGCA CAGCGTCTAA GATCCTGAAC AGCCAGCAGC AGATGAACGT GCTGGACCAA 
ACCAAGACTC TGGCTGAGTC TGCGCTACAG ATGCTCTATA CGGCCAAAGA GACTGGAGGA 
AACCACAAGG CGGCGCACAC ACAAGAGGCT CTGGAGGAGT CTGTTCAGAT GATGAAGGAG 
GCGGTGGACG ATCTGGGTTC GACTCTGGCA GAAGTGGCCA GCGCTGCAGG AGCAGTGGGA 
GGAATGGTCA ACTCCATCAC TCAGTCCATC AACAAGATGG AGGACGGACC TGCCTTCGAG 
CCTGAAGGAA CGTTTGTGGA TTATCAGACC ACCATGGTGA AGACAGCCAA AGCTATCGCT 
GTCACCGTTC AGGAGATGGT TACCAAATCC AACACCAACC CTGATGAGCT GGGCGGTTTG 
GCCAATCAGC TGACCACAGA GTTCGGTGAC CTCGCATCAG AGGCCAAATG TGCAGCCATG 
ACTGCTGAAA ACGATGAGAT CGGTTCACAT ATCAAGAAGC AGGTGACTGA GTTGGGCTAC 
ACCTGCACTG GTCTGGTCAG TAAAGCAGGA GCGCTACAGT GCAGTCCAAA CGACTCCTAC 
ACTAAGAAGG AGCTCATTGA CAGTGCCAGG AAAGTCTCTG AGAAGGTCTC TCATGTGCTG 
GCAGCTCTTC AGGCAGGCAA TCGTGGCACT CAGGCCTGCA TTACTGCCGC GAGCGCTGTC 
TCTGGCATCA TTGCTGATTT AGACACCACC ATCATGTTTG CCACCGCGGG CACTCTCAAC 
CGAGAGAACG CAGAGACATT TGCAGACCAC AGAGAGCACA TCTTAAAGAC GGCTAAAGTT 
CTGGTGGAGG ACACGAAGCT GCTGGTTTCT GGAGCCGGAG CAAGTCAGGA GAAACTGGCT 
CAGGCTGCAC AGTCCTCCGT CAACACCATC ACCAAACTGG CCGATGTGGT TAAATTGGGG 
GCAGCGAGTC TGGGCTCTGA AGACCCAGAG ACACAGGTGG TTCTGATCAA CGCTGTGAAG 
GACGTGGCTA AAGCTCTGGG TGACCTGATC AGCGCCACCA AAGCAGCGGC CGGCAAACCT 
CACGATGACC CCGCCATGCT GCAGCTCAAA AACTCTGCTA AGGTGATGGT GACCAATGTG 
ACGTCTCTAC TTAAAACCGT GAAAGCCGTG GAGGATGAAG CCACTAAAGG CACACGAGCA 
CTAGAGGCCA CCATAGAGCA CATCAAACAA GAGCTGGCTG TTTTCAGCAG TGCAGATCCT 
CCTCCAAAGA CGGCCACTCC AGAGGAGTTC ATCCGCATGA CCAAAGGCAT CACATTGGCC 
ACAGCTAAAG CAGTGGCTGC TGGGAACTCC TGCCGTCAGG AGGACGTGAT CGCCACTGCT 
AACCTAAGCA GACGAGCTGT CGCAGACATG CTGCGCTCCT GCAAGCAAGC CGCATACCAC 
CCAGAGGTGA AGCGGGAGGT CCAGACTCGA GCGCTCCGCT TTGGCTCTGA GTGCTCCACA 
AGTTACCTGG GCCTGCTGGA GCACGTGTTA GTGATCATTC AGAAACCCTC GCATGACCTT 
AAACAGCAGC TGGCAAACTA CTCCAAACGT GTGGCCAGTT CAGTGACCGA GCTCATCCAG 
GCTGCGGAGG CCATGAAAGG TACGGAGTGG GTGGATCCCG AAGACCCCAC CGTCATTGCA 
GAAAACGAGC TGCTGGGAGC CGCAGCTGCC ATTGAAGCTG CCGCGAAGAA ACTAGAGCAG 
CTCAAACCCC GAGCCAAACC CAAGGAAGCT GATGAGAGTC TGAACTTTGA GGAACAGATT 
CTAGAAGCGG CCAAATCCAT CGCTGCAGCC ACCAGTGCTC TGGTCAAAGC TGCGTCTGCC 
GCTCAGAGAG AGCTGGTGGC TCAAGGGAAG GTTGGGGCAA TTCCAGCAAA TGCGGTGGAT 
GATGGACAGT GGTCTCAGGG TCTCATTTCA GCTGCTCGTA TGGTAGCTGC AGCTACTAAC 
AACCTGTGCG AAGCAGCTAA CTCTGCGGTT CAGGGTCACG CCAGCGAGGA GAAACTCATT 
TCTTCAGCCA AACAGGTGGC AGCATCCACC GCACAGCTGC TGGTGGCCTG TAAGGTCAAG 
GCTGACCAGG ACTCCCAGAC CATGAAGAGA CTGCAGGCTG CTGGTAATGC TGTCAAGAGG 
GCGTCTGATA ACCTGGTGAA GGCGGCACAG AAGGCTGCGT TTGAGGCTCA AGATGATCAG 
GCTGTGATGG TGAAGAGCAA GATGGTGGGC GGTATTGCTC AGATCATCGC TGCTCAGGAG 
GAGATGCTCA GGAAGGAGAA AGAGCTGGAA GAAGCCAGAA AGAAACTGGC AATGATCCGA 
CAGCAGCAGT ACAAGTTCCT GCCCTCAGAG CTGCGAGAGG ACGGACAGGA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tln1 ( XM_005155499.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155556.1 Danio rerio talin 1 (tln1), transcript variant X1, mRNA.

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CloneID	ODa15805
Clone ID Related Accession (Same CDS sequence)	XM_005155499.3 , XM_005155499.4
Accession Version	XM_005155499.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	talin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155499.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGTCTGT AGGTACAGCA GCGTTCACAC ACTCCAGACG GACAGGCTAT 
AGTCAAATGC TGGACATGTT GATGCAGTTG GCGAAGGCTG TGGCGAATGC AGCAGCGGCT 
CTGGTGTTAA AGGCTAAAAA CGTGGCTCAG AAAAGCGAAG ATTCAGCTCA ACAAAACAAA 
GTGATCGCTG CTGCCACCCA GTGCGCCCTC TCCACATCAC AGCTGGTGGC CTGCACACGA 
GTGGTGGCTC CAACCATCAG CTCACCCGTG TGTCAAGAGC AGCTCATCGA GGCTGGAAAA 
CTGGTGGCGA AGTCTGTGGA GGGCTGTGTG GAGGCGTCAC ACGGGGCGAC CGGTGACGAG 
CATCTGCTCA AACAGGTGGG CGCCGCTGCC ACAGGAGTCA CTCAGGCTCT TAACGAGCTG 
CTGCAGCATA TTAGACAGTA CGCCAGCGGA GGACAGCCAA TCGGACGGCA CGGAGAGGCT 
ACCGACCGCA TCCTGGATGT GACTGATAAC ATCTTCAGCT CCATGGGAGA TGCAGGGGAG 
ATGGTGCGTC AGGCGCGGAT CCTTGCTCAG GCCACCTCAG ACCTGGTCAA CGCGATCAAG 
GCGGACGCAG AGGGAGAATC TGACCTGGAG AACTCTCGCA AACTCCTCTC TGCAGCCAAA 
CTCCTGGCTG ACGCTACTGC CCGTATGGTG GAAGCTGCGA AGGGTGCAGC TGCTAATCCA 
GACAGCGAGG AGCAGCAGCA GAAGTTGCGT GAAGCTGCAG AGGGACTGCG CATGGCCACC 
AATGCTGCAG CACAGAACGC CATTAAAAAG AGACTCGTCA ACAAACTGGA GAATGCAGCG 
AAACAAGCCG CAGCAGCCGC CACTCAGACC ATAGCAGCCG CTCAACATGC AGCTTCATCC 
AACAAGAACC CTGCGGCCCA GCAACAACTC GTACAGAGCT GCAAGGTGGT GGCGGATCAG 
ATCCCACAGC TGGTTCAGGG TCTGAGGAGC AGCCAGGCTC AACCTGACAG CCCCAGCGCT 
CAACTCGCCC TCATCGGAGC TAGCCAGAAC TTCCTGCAGC CCGGAGCCAA AATGGTGACC 
GCAGCCAAAG CTACAGTTCC AACCATCAGT GACCAGGCGT CTGCCATGCA GCTCAGTCAG 
TGCGCTAAAA ACCTGGCCAG TGCTCTGGCT GAGCTCCGCA CGGCCGCCCA GAAGGCTCAG 
GAGGCGTGTG GTCCGTTGGA GATTGATAAC GCTCTGACTA TGGTACGGGG GCTGGAGAGG 
GACATGCAGG AGGCCAAAGC TTCAGCTGCT GAGGGCAAAC TCAGACCCCT GCCTGGAGAA 
ACGCTGGAGA AGTGCTCTCA GGATCTGGGC TCCAGCACTA AAGCCGTGAG CTCTGCCATT 
GCTCAGCTCC TCAGTGAAGC AACTCAAGGC AATGAGAACT ACACAGGTAT GGCTGCTCGT 
GATGTGGCTC AGGCTCTGCG CTCGCTGGCT TCAGCCGCTC GTGGAGTGGC AGCCAACACT 
GAAGATCCTC TAGCCCGTAA CGCAATGCTG GACTGCACCG GCGATGTCAT GGACAAATCC 
GCCAGCCTGA TTGAAGAGAC CAAGAGGGCC ATAGCCAAAC CAGGAGACCC TGACAGTCAG 
CAAAGACTGG CCCAGGTAGC CAAGGCTGTG TCTCAGGCAC TGAACCGCTC TGTAAACTGT 
CTGCCGGGGC AGAGGGATGT GGATAATGCC ATCAGAACTG TCGGGGAAGC TAGCAAGAAA 
CTGCTGTCTG ATAAATTTCC AGCGAGTGGG AAGAATTTCC AAGAGGCTCA GGCCAATCTG 
AACGAGGCTG CAGCTGGACT AAACCAGTCA GCCAATGAGC TGGTGCAGGC CTCGCGTGGC 
ACCACCCAGG ACCTGGCCAA AGCCTCCGGG AAGTTTGGAC AAGACTTTAA CCAATTCCTG 
CAGGCTGGAG TGGACATGGC TGGACAATCC CAGTCTAAAG AAGACCAAAC TCAAGTGGTC 
ACCAACCTGA AGACCATCTC CATGTCCTCC AGCAAACTCC TGCTGGCGGC TAAAGCTCTG 
TCCACAGACC CCAACTCGCC AAATCTGAAG AACCAACTCG CTGCTGCTGC TCGCGCGGTG 
ACAGACAGCA TTAATCAGCT GATCAGCATG TGTACCCAGC AGGCTCCGGG ACAGAAGGAG 
TGTGACAACG CTCTCCGTGA GCTTGAGACG GTTGGAGGAA TGCTGGAGAA CCCAACAGAA 
GCAGTCAATG ATTTGGGCTA CTTTGACTGC ATTGACAGTG TGATGGAGAA CTCAAAGGTT 
TTGGGTGAGG CCATGGCTGG CATTTCCCAT AATGCCAAAA ACAGTAACTT GCCAGAGTTT 
GGAGATTCGG TCAGTTCAGC GTCTAAAGCG CTGTGTGGTC TCACTGAAGC AGCTGCTCAG 
GGGGCGTATC TGGTTGGTGT GTCAGACCCG AACAGCCATG CTGGCCAGAA GGGTCTTGTG 
GACCCCTCAC AATTTGCCAG AGCCAAACAG TCCATCCAGA TGGCCTGTCA GAACCTGGTG 
GATCCAGCCT GCACCCAGTC CCAGGTTCTC TCTGCTGCCA CCATCGTTGC CAAACACACT 
TCGGCCTTGT GCAATGCCTG CCGGCTCGCC TCCTCCAAGA CATCCAACCC AGTTGCTAAG 
AGACAGTTCG TCCAGAGTGC CAAAGAGGTG GCCAACAGCA CAGCCAACCT CGTCAAGTCC 
ATCAAGGCTC TCGACGGGGC CTTTAACCAG GAAAACAGAC AGAAGTGTAA GGAAGCCACT 
GGGCCACTCA TTGAGGCTGT GGATAACCTG ACTGCCTTCG CTTCAAACCC AGAGTTCGCA 
AGTGTACCCG CTCAGATCAG CCCAGAGGGT CTGGCTGCGA TGGAGCCGAT CACAGCAGCC 
GCTAAGGCAA TGTTGGAAAG CTCGACAGGA CTGATCCAGA CGGCTCGCTC GCTCGCAGTC 
AACCCGAAAG ATCCACCCAA GTGGTCTGTG CTTGCTGGAC ACTCCAGAAC AGTGTCCGAC 
TCCATCAAGA AACTCATCAC TAACATGAGG GAAAAGGCTC CGGGTCAGAG GGAGTGTGAT 
GATGCCATTG AGGTTCTTAG TAACTGCATT CGTGAAGTGG ACAAGGCCTC GCTGGCAGCC 
ATCAGTCAGC AGCTCACGCC TAGAGACGAC ATTTCCCATG AGGCTCTGCA CGAGCAGATG 
GCGGCGTCTG TGCATGAGAT CAGTAACCTG ATTGAACCAG TGGCCATCGC AGCCCGTGCT 
GATGCCTCTC AGCTGGGACA CAAGGTGTCT CAGATGGTCA GTTACTTTGA ACCGCTCATC 
ATGGCTGCCA TCGGCACAGC GTCTAAGATC CTGAACAGCC AGCAGCAGAT GAACGTGCTG 
GACCAAACCA AGACTCTGGC TGAGTCTGCG CTACAGATGC TCTATACGGC CAAAGAGACT 
GGAGGAAACC ACAAGGCGGC GCACACACAA GAGGCTCTGG AGGAGTCTGT TCAGATGATG 
AAGGAGGCGG TGGACGATCT GGGTTCGACT CTGGCAGAAG TGGCCAGCGC TGCAGGAGCA 
GTGGGAGGAA TGGTCAACTC CATCACTCAG TCCATCAACA AGATGGAGGA CGGACCTGCC 
TTCGAGCCTG AAGGAACGTT TGTGGATTAT CAGACCACCA TGGTGAAGAC AGCCAAAGCT 
ATCGCTGTCA CCGTTCAGGA GATGGTTACC AAATCCAACA CCAACCCTGA TGAGCTGGGC 
GGTTTGGCCA ATCAGCTGAC CACAGAGTTC GGTGACCTCG CATCAGAGGC CAAATGTGCA 
GCCATGACTG CTGAAAACGA TGAGATCGGT TCACATATCA AGAAGCAGGT GACTGAGTTG 
GGCTACACCT GCACTGGTCT GGTCAGTAAA GCAGGAGCGC TACAGTGCAG TCCAAACGAC 
TCCTACACTA AGAAGGAGCT CATTGACAGT GCCAGGAAAG TCTCTGAGAA GGTCTCTCAT 
GTGCTGGCAG CTCTTCAGGC AGGCAATCGT GGCACTCAGG CCTGCATTAC TGCCGCGAGC 
GCTGTCTCTG GCATCATTGC TGATTTAGAC ACCACCATCA TGTTTGCCAC CGCGGGCACT 
CTCAACCGAG AGAACGCAGA GACATTTGCA GACCACAGAG AGCACATCTT AAAGACGGCT 
AAAGTTCTGG TGGAGGACAC GAAGCTGCTG GTTTCTGGAG CCGGAGCAAG TCAGGAGAAA 
CTGGCTCAGG CTGCACAGTC CTCCGTCAAC ACCATCACCA AACTGGCCGA TGTGGTTAAA 
TTGGGGGCAG CGAGTCTGGG CTCTGAAGAC CCAGAGACAC AGGTGGTTCT GATCAACGCT 
GTGAAGGACG TGGCTAAAGC TCTGGGTGAC CTGATCAGCG CCACCAAAGC AGCGGCCGGC 
AAACCTCACG ATGACCCCGC CATGCTGCAG CTCAAAAACT CTGCTAAGGT GATGGTGACC 
AATGTGACGT CTCTACTTAA GACGGTGAAG GCGGTGGAGG ATGAAGCCAC TAAAGGCACA 
CGAGCACTAG AGGCCACCAT AGAGCACATC AAACAAGAGC TGGCTGTTTT CAGCAGTGCA 
GATCCTCCTC CAAAGACGGC CACTCCAGAG GAGTTCATCC GCATGACCAA AGGCATCACA 
TTGGCCACAG CTAAAGCAGT GGCTGCTGGG AACTCCTGCC GTCAGGAGGA CGTGATCGCC 
ACTGCTAACC TAAGCAGACG AGCTGTCGCA GACATGCTGC GCTCCTGCAA GCAAGCCGCA 
TACCACCCGG AGGTGAAGCG GGAGGTCCAG ACTCGAGCGC TCCGCTTTGG CTCTGAGTGC 
TCCACAAGTT ACCTGGGCCT GCTGGAGCAC GTGTTAGTGA TCATTCAGAA ACCCTCGCAT 
GACCTTAAAC AGCAGCTGGC AAACTACTCC AAACGTGTGG CCAGTTCAGT GACCGAGCTC 
ATCCAGGCTG CGGAGGCCAT GAAAGGTACG GAGTGGGTGG ATCCCGAAGA CCCCACCGTC 
ATTGCAGAAA ACGAGCTGCT GGGAGCCGCA GCTGCCATTG AAGCTGCCGC GAAGAAACTA 
GAGCAGCTCA AACCCCGAGC CAAACCCAAG GAAGCTGATG AGAGTCTGAA CTTTGAGGAA 
CAGATTCTAG AAGCGGCCAA ATCCATCGCT GCAGCCACCA GTGCTCTGGT CAAAGCTGCG 
TCTGCCGCTC AGAGAGAGCT GGTGGCTCAA GGGAAGGTTG GGGCAATTCC AGCAAACGCG 
GTGGATGATG GACAGTGGTC TCAGGGTCTC ATTTCAGCTG CTCGTATGGT AGCCGCAGCT 
ACTAACAACC TGTGCGAAGC AGCTAACTCT GCGGTTCAGG GTCACGCCAG CGAGGAGAAA 
CTCATTTCTT CAGCCAAACA GGTGGCAGCA TCCACCGCAC AGCTGCTGGT GGCCTGTAAG 
GTCAAGGCTG ACCAGGACTC CCAGACCATG AAGAGACTGC AGGCTGCTGG TAATGCTGTC 
AAGAGGGCGT CTGATAACCT GGTGAAGGCG GCACAGAAGG CTGCGTTTGA GGCTCAAGAT 
GATCAGGCTG TGATGGTGAA GAGCAAGATG GTGGGCGGTA TTGCTCAGAT CATCGCTGCT 
CAGGAGGAGA TGCTCAGGAA GGAGAAAGAG CTGGAAGAAG CCAGAAAGAA ACTGGCAATG 
ATCCGACAGC AGCAGTACAA GTTCCTGCCC TCAGAGCTGC GAGAGGACGG ACAGGAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155556.1
CDS	196..7878
Translation	MVALSLKIGVGNVVKTMQFEPSTVVYDACRIIRERVPEAQLGQPSDYGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTLEYKKKQRPLKIRMLDGTVKTVMVDDSKIVSDMLMTICARIGITNYDEYSLVRDVGEEKKEETTGTLKRDKTLLRDDKKMEKLKQKLHTDDELNWLDHGRTLREQGVEESEMLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGYQCQIQFGDHNESKHKPGFLDLKEFLPKEYVKNKGEKRIFQAHKNCQNMTEIEAKVNYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKESVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQFNKMGKVETGSVALPAIIRSGAGGPESMKMGSMPQAKQHITSGQMHRGHMPPLTSAQQALTGTINSSMQAVQAVQASLDEFDALPPLGTDSASQAWRKNKMDESKHEIHSQVDAITAGTASMVNLTAGDPAETDYTAVGCAVTTISSNLTEMSKGVKLLAALMEDEGGNGQQLLGAAKNLACAVSDMLKTAQPSSTEPRQNLLQAAGNVGQASGELLQQIGESDADQHFQSVGTAAFTHSRRTGYSQMLDMLMQLAKAVANAAAALVLKAKNVAQKSEDSAQQNKVIAAATQCALSTSQLVACTRVVAPTISSPVCQEQLIEAGKLVAKSVEGCVEASHGATGDEHLLKQVGAAATGVTQALNELLQHIRQYASGGQPIGRHGEATDRILDVTDNIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGESDLENSRKLLSAAKLLADATARMVEAAKGAAANPDSEEQQQKLREAAEGLRMATNAAAQNAIKKRLVNKLENAAKQAAAAATQTIAAAQHAASSNKNPAAQQQLVQSCKVVADQIPQLVQGLRSSQAQPDSPSAQLALIGASQNFLQPGAKMVTAAKATVPTISDQASAMQLSQCAKNLASALAELRTAAQKAQEACGPLEIDNALTMVRGLERDMQEAKASAAEGKLRPLPGETLEKCSQDLGSSTKAVSSAIAQLLSEATQGNENYTGMAARDVAQALRSLASAARGVAANTEDPLARNAMLDCTGDVMDKSASLIEETKRAIAKPGDPDSQQRLAQVAKAVSQALNRSVNCLPGQRDVDNAIRTVGEASKKLLSDKFPASGKNFQEAQANLNEAAAGLNQSANELVQASRGTTQDLAKASGKFGQDFNQFLQAGVDMAGQSQSKEDQTQVVTNLKTISMSSSKLLLAAKALSTDPNSPNLKNQLAAAARAVTDSINQLISMCTQQAPGQKECDNALRELETVGGMLENPTEAVNDLGYFDCIDSVMENSKVLGEAMAGISHNAKNSNLPEFGDSVSSASKALCGLTEAAAQGAYLVGVSDPNSHAGQKGLVDPSQFARAKQSIQMACQNLVDPACTQSQVLSAATIVAKHTSALCNACRLASSKTSNPVAKRQFVQSAKEVANSTANLVKSIKALDGAFNQENRQKCKEATGPLIEAVDNLTAFASNPEFASVPAQISPEGLAAMEPITAAAKAMLESSTGLIQTARSLAVNPKDPPKWSVLAGHSRTVSDSIKKLITNMREKAPGQRECDDAIEVLSNCIREVDKASLAAISQQLTPRDDISHEALHEQMAASVHEISNLIEPVAIAARADASQLGHKVSQMVSYFEPLIMAAIGTASKILNSQQQMNVLDQTKTLAESALQMLYTAKETGGNHKAAHTQEALEESVQMMKEAVDDLGSTLAEVASAAGAVGGMVNSITQSINKMEDGPAFEPEGTFVDYQTTMVKTAKAIAVTVQEMVTKSNTNPDELGGLANQLTTEFGDLASEAKCAAMTAENDEIGSHIKKQVTELGYTCTGLVSKAGALQCSPNDSYTKKELIDSARKVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNRENAETFADHREHILKTAKVLVEDTKLLVSGAGASQEKLAQAAQSSVNTITKLADVVKLGAASLGSEDPETQVVLINAVKDVAKALGDLISATKAAAGKPHDDPAMLQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATIEHIKQELAVFSSADPPPKTATPEEFIRMTKGITLATAKAVAAGNSCRQEDVIATANLSRRAVADMLRSCKQAAYHPEVKREVQTRALRFGSECSTSYLGLLEHVLVIIQKPSHDLKQQLANYSKRVASSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATNNLCEAANSAVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSQTMKRLQAAGNAVKRASDNLVKAAQKAAFEAQDDQAVMVKSKMVGGIAQIIAAQEEMLRKEKELEEARKKLAMIRQQQYKFLPSELREDGQEQ

Target ORF information:

RefSeq Version	XM_005155499.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio talin 1 (tln1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155499.3

ORF Insert Sequence:

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGTCTGT AGGTACAGCA GCGTTCACAC ACTCCAGACG GACAGGCTAT 
AGTCAAATGC TGGACATGTT GATGCAGTTG GCGAAGGCTG TGGCGAATGC AGCAGCGGCT 
CTGGTGTTAA AGGCTAAAAA CGTGGCTCAG AAAAGCGAAG ATTCAGCTCA ACAAAACAAA 
GTGATCGCTG CTGCCACCCA GTGCGCCCTC TCCACATCAC AGCTGGTGGC CTGCACACGA 
GTGGTGGCTC CAACCATCAG CTCACCCGTG TGTCAAGAGC AGCTCATCGA GGCTGGAAAA 
CTGGTGGCGA AGTCTGTGGA GGGCTGTGTG GAGGCGTCAC ACGGGGCGAC CGGTGACGAG 
CATCTGCTCA AACAGGTGGG CGCCGCTGCC ACAGGAGTCA CTCAGGCTCT TAACGAGCTG 
CTGCAGCATA TTAGACAGTA CGCCAGCGGA GGACAGCCAA TCGGACGGCA CGGAGAGGCT 
ACCGACCGCA TCCTGGATGT GACTGATAAC ATCTTCAGCT CCATGGGAGA TGCAGGGGAG 
ATGGTGCGTC AGGCGCGGAT CCTTGCTCAG GCCACCTCAG ACCTGGTCAA CGCGATCAAG 
GCGGACGCAG AGGGAGAATC TGACCTGGAG AACTCTCGCA AACTCCTCTC TGCAGCCAAA 
CTCCTGGCTG ACGCTACTGC CCGTATGGTG GAAGCTGCGA AGGGTGCAGC TGCTAATCCA 
GACAGCGAGG AGCAGCAGCA GAAGTTGCGT GAAGCTGCAG AGGGACTGCG CATGGCCACC 
AATGCTGCAG CACAGAACGC CATTAAAAAG AGACTCGTCA ACAAACTGGA GAATGCAGCG 
AAACAAGCCG CAGCAGCCGC CACTCAGACC ATAGCAGCCG CTCAACATGC AGCTTCATCC 
AACAAGAACC CTGCGGCCCA GCAACAACTC GTACAGAGCT GCAAGGTGGT GGCGGATCAG 
ATCCCACAGC TGGTTCAGGG TCTGAGGAGC AGCCAGGCTC AACCTGACAG CCCCAGCGCT 
CAACTCGCCC TCATCGGAGC TAGCCAGAAC TTCCTGCAGC CCGGAGCCAA AATGGTGACC 
GCAGCCAAAG CTACAGTTCC AACCATCAGT GACCAGGCGT CTGCCATGCA GCTCAGTCAG 
TGCGCTAAAA ACCTGGCCAG TGCTCTGGCT GAGCTCCGCA CGGCCGCCCA GAAGGCTCAG 
GAGGCGTGTG GTCCGTTGGA GATTGATAAC GCTCTGACTA TGGTACGGGG GCTGGAGAGG 
GACATGCAGG AGGCCAAAGC TTCAGCTGCT GAGGGCAAAC TCAGACCCCT GCCTGGAGAA 
ACGCTGGAGA AGTGCTCTCA GGATCTGGGC TCCAGCACTA AAGCCGTGAG CTCTGCCATT 
GCTCAGCTCC TCAGTGAAGC AACTCAAGGC AATGAGAACT ACACAGGTAT GGCTGCTCGT 
GATGTGGCTC AGGCTCTGCG CTCGCTGGCT TCAGCCGCTC GTGGAGTGGC AGCCAACACT 
GAAGATCCTC TAGCCCGTAA CGCAATGCTG GACTGCACCG GCGATGTCAT GGACAAATCC 
GCCAGCCTGA TTGAAGAGAC CAAGAGGGCC ATAGCCAAAC CAGGAGACCC TGACAGTCAG 
CAAAGACTGG CCCAGGTAGC CAAGGCTGTG TCTCAGGCAC TGAACCGCTC TGTAAACTGT 
CTGCCGGGGC AGAGGGATGT GGATAATGCC ATCAGAACTG TCGGGGAAGC TAGCAAGAAA 
CTGCTGTCTG ATAAATTTCC AGCGAGTGGG AAGAATTTCC AAGAGGCTCA GGCCAATCTG 
AACGAGGCTG CAGCTGGACT AAACCAGTCA GCCAATGAGC TGGTGCAGGC CTCGCGTGGC 
ACCACCCAGG ACCTGGCCAA AGCCTCCGGG AAGTTTGGAC AAGACTTTAA CCAATTCCTG 
CAGGCTGGAG TGGACATGGC TGGACAATCC CAGTCTAAAG AAGACCAAAC TCAAGTGGTC 
ACCAACCTGA AGACCATCTC CATGTCCTCC AGCAAACTCC TGCTGGCGGC TAAAGCTCTG 
TCCACAGACC CCAACTCGCC AAATCTGAAG AACCAACTCG CTGCTGCTGC TCGCGCGGTG 
ACAGACAGCA TTAATCAGCT GATCAGCATG TGTACCCAGC AGGCTCCGGG ACAGAAGGAG 
TGTGACAACG CTCTCCGTGA GCTTGAGACG GTTGGAGGAA TGCTGGAGAA CCCAACAGAA 
GCAGTCAATG ATTTGGGCTA CTTTGACTGC ATTGACAGTG TGATGGAGAA CTCAAAGGTT 
TTGGGTGAGG CCATGGCTGG CATTTCCCAT AATGCCAAAA ACAGTAACTT GCCAGAGTTT 
GGAGATTCGG TCAGTTCAGC GTCTAAAGCG CTGTGTGGTC TCACTGAAGC AGCTGCTCAG 
GGGGCGTATC TGGTTGGTGT GTCAGACCCG AACAGCCATG CTGGCCAGAA GGGTCTTGTG 
GACCCCTCAC AATTTGCCAG AGCCAAACAG TCCATCCAGA TGGCCTGTCA GAACCTGGTG 
GATCCAGCCT GCACCCAGTC CCAGGTTCTC TCTGCTGCCA CCATCGTTGC CAAACACACT 
TCGGCCTTGT GCAATGCCTG CCGGCTCGCC TCCTCCAAGA CATCCAACCC AGTTGCTAAG 
AGACAGTTCG TCCAGAGTGC CAAAGAGGTG GCCAACAGCA CAGCCAACCT CGTCAAGTCC 
ATCAAGGCTC TCGACGGGGC CTTTAACCAG GAAAACAGAC AGAAGTGTAA GGAAGCCACT 
GGGCCACTCA TTGAGGCTGT GGATAACCTG ACTGCCTTCG CTTCAAACCC AGAGTTCGCA 
AGTGTACCCG CTCAGATCAG CCCAGAGGGT CTGGCTGCGA TGGAGCCGAT CACAGCAGCC 
GCTAAGGCAA TGTTGGAAAG CTCGACAGGA CTGATCCAGA CGGCTCGCTC GCTCGCAGTC 
AACCCGAAAG ATCCACCCAA GTGGTCTGTG CTTGCTGGAC ACTCCAGAAC AGTGTCCGAC 
TCCATCAAGA AACTCATCAC TAACATGAGG GAAAAGGCTC CGGGTCAGAG GGAGTGTGAT 
GATGCCATTG AGGTTCTTAG TAACTGCATT CGTGAAGTGG ACAAGGCCTC GCTGGCAGCC 
ATCAGTCAGC AGCTCACGCC TAGAGACGAC ATTTCCCATG AGGCTCTGCA CGAGCAGATG 
GCGGCGTCTG TGCATGAGAT CAGTAACCTG ATTGAACCAG TGGCCATCGC AGCCCGTGCT 
GATGCCTCTC AGCTGGGACA CAAGGTGTCT CAGATGGTCA GTTACTTTGA ACCGCTCATC 
ATGGCTGCCA TCGGCACAGC GTCTAAGATC CTGAACAGCC AGCAGCAGAT GAACGTGCTG 
GACCAAACCA AGACTCTGGC TGAGTCTGCG CTACAGATGC TCTATACGGC CAAAGAGACT 
GGAGGAAACC ACAAGGCGGC GCACACACAA GAGGCTCTGG AGGAGTCTGT TCAGATGATG 
AAGGAGGCGG TGGACGATCT GGGTTCGACT CTGGCAGAAG TGGCCAGCGC TGCAGGAGCA 
GTGGGAGGAA TGGTCAACTC CATCACTCAG TCCATCAACA AGATGGAGGA CGGACCTGCC 
TTCGAGCCTG AAGGAACGTT TGTGGATTAT CAGACCACCA TGGTGAAGAC AGCCAAAGCT 
ATCGCTGTCA CCGTTCAGGA GATGGTTACC AAATCCAACA CCAACCCTGA TGAGCTGGGC 
GGTTTGGCCA ATCAGCTGAC CACAGAGTTC GGTGACCTCG CATCAGAGGC CAAATGTGCA 
GCCATGACTG CTGAAAACGA TGAGATCGGT TCACATATCA AGAAGCAGGT GACTGAGTTG 
GGCTACACCT GCACTGGTCT GGTCAGTAAA GCAGGAGCGC TACAGTGCAG TCCAAACGAC 
TCCTACACTA AGAAGGAGCT CATTGACAGT GCCAGGAAAG TCTCTGAGAA GGTCTCTCAT 
GTGCTGGCAG CTCTTCAGGC AGGCAATCGT GGCACTCAGG CCTGCATTAC TGCCGCGAGC 
GCTGTCTCTG GCATCATTGC TGATTTAGAC ACCACCATCA TGTTTGCCAC CGCGGGCACT 
CTCAACCGAG AGAACGCAGA GACATTTGCA GACCACAGAG AGCACATCTT AAAGACGGCT 
AAAGTTCTGG TGGAGGACAC GAAGCTGCTG GTTTCTGGAG CCGGAGCAAG TCAGGAGAAA 
CTGGCTCAGG CTGCACAGTC CTCCGTCAAC ACCATCACCA AACTGGCCGA TGTGGTTAAA 
TTGGGGGCAG CGAGTCTGGG CTCTGAAGAC CCAGAGACAC AGGTGGTTCT GATCAACGCT 
GTGAAGGACG TGGCTAAAGC TCTGGGTGAC CTGATCAGCG CCACCAAAGC AGCGGCCGGC 
AAACCTCACG ATGACCCCGC CATGCTGCAG CTCAAAAACT CTGCTAAGGT GATGGTGACC 
AATGTGACGT CTCTACTTAA GACGGTGAAG GCGGTGGAGG ATGAAGCCAC TAAAGGCACA 
CGAGCACTAG AGGCCACCAT AGAGCACATC AAACAAGAGC TGGCTGTTTT CAGCAGTGCA 
GATCCTCCTC CAAAGACGGC CACTCCAGAG GAGTTCATCC GCATGACCAA AGGCATCACA 
TTGGCCACAG CTAAAGCAGT GGCTGCTGGG AACTCCTGCC GTCAGGAGGA CGTGATCGCC 
ACTGCTAACC TAAGCAGACG AGCTGTCGCA GACATGCTGC GCTCCTGCAA GCAAGCCGCA 
TACCACCCGG AGGTGAAGCG GGAGGTCCAG ACTCGAGCGC TCCGCTTTGG CTCTGAGTGC 
TCCACAAGTT ACCTGGGCCT GCTGGAGCAC GTGTTAGTGA TCATTCAGAA ACCCTCGCAT 
GACCTTAAAC AGCAGCTGGC AAACTACTCC AAACGTGTGG CCAGTTCAGT GACCGAGCTC 
ATCCAGGCTG CGGAGGCCAT GAAAGGTACG GAGTGGGTGG ATCCCGAAGA CCCCACCGTC 
ATTGCAGAAA ACGAGCTGCT GGGAGCCGCA GCTGCCATTG AAGCTGCCGC GAAGAAACTA 
GAGCAGCTCA AACCCCGAGC CAAACCCAAG GAAGCTGATG AGAGTCTGAA CTTTGAGGAA 
CAGATTCTAG AAGCGGCCAA ATCCATCGCT GCAGCCACCA GTGCTCTGGT CAAAGCTGCG 
TCTGCCGCTC AGAGAGAGCT GGTGGCTCAA GGGAAGGTTG GGGCAATTCC AGCAAACGCG 
GTGGATGATG GACAGTGGTC TCAGGGTCTC ATTTCAGCTG CTCGTATGGT AGCCGCAGCT 
ACTAACAACC TGTGCGAAGC AGCTAACTCT GCGGTTCAGG GTCACGCCAG CGAGGAGAAA 
CTCATTTCTT CAGCCAAACA GGTGGCAGCA TCCACCGCAC AGCTGCTGGT GGCCTGTAAG 
GTCAAGGCTG ACCAGGACTC CCAGACCATG AAGAGACTGC AGGCTGCTGG TAATGCTGTC 
AAGAGGGCGT CTGATAACCT GGTGAAGGCG GCACAGAAGG CTGCGTTTGA GGCTCAAGAT 
GATCAGGCTG TGATGGTGAA GAGCAAGATG GTGGGCGGTA TTGCTCAGAT CATCGCTGCT 
CAGGAGGAGA TGCTCAGGAA GGAGAAAGAG CTGGAAGAAG CCAGAAAGAA ACTGGCAATG 
ATCCGACAGC AGCAGTACAA GTTCCTGCCC TCAGAGCTGC GAGAGGACGG ACAGGAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tln1 ( XM_005155499.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155556.1 Danio rerio talin 1 (tln1), transcript variant X2, mRNA.

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CloneID	ODa15805
Clone ID Related Accession (Same CDS sequence)	XM_005155499.3 , XM_005155499.4
Accession Version	XM_005155499.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	talin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155499.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGTCTGT AGGTACAGCA GCGTTCACAC ACTCCAGACG GACAGGCTAT 
AGTCAAATGC TGGACATGTT GATGCAGTTG GCGAAGGCTG TGGCGAATGC AGCAGCGGCT 
CTGGTGTTAA AGGCTAAAAA CGTGGCTCAG AAAAGCGAAG ATTCAGCTCA ACAAAACAAA 
GTGATCGCTG CTGCCACCCA GTGCGCCCTC TCCACATCAC AGCTGGTGGC CTGCACACGA 
GTGGTGGCTC CAACCATCAG CTCACCCGTG TGTCAAGAGC AGCTCATCGA GGCTGGAAAA 
CTGGTGGCGA AGTCTGTGGA GGGCTGTGTG GAGGCGTCAC ACGGGGCGAC CGGTGACGAG 
CATCTGCTCA AACAGGTGGG CGCCGCTGCC ACAGGAGTCA CTCAGGCTCT TAACGAGCTG 
CTGCAGCATA TTAGACAGTA CGCCAGCGGA GGACAGCCAA TCGGACGGCA CGGAGAGGCT 
ACCGACCGCA TCCTGGATGT GACTGATAAC ATCTTCAGCT CCATGGGAGA TGCAGGGGAG 
ATGGTGCGTC AGGCGCGGAT CCTTGCTCAG GCCACCTCAG ACCTGGTCAA CGCGATCAAG 
GCGGACGCAG AGGGAGAATC TGACCTGGAG AACTCTCGCA AACTCCTCTC TGCAGCCAAA 
CTCCTGGCTG ACGCTACTGC CCGTATGGTG GAAGCTGCGA AGGGTGCAGC TGCTAATCCA 
GACAGCGAGG AGCAGCAGCA GAAGTTGCGT GAAGCTGCAG AGGGACTGCG CATGGCCACC 
AATGCTGCAG CACAGAACGC CATTAAAAAG AGACTCGTCA ACAAACTGGA GAATGCAGCG 
AAACAAGCCG CAGCAGCCGC CACTCAGACC ATAGCAGCCG CTCAACATGC AGCTTCATCC 
AACAAGAACC CTGCGGCCCA GCAACAACTC GTACAGAGCT GCAAGGTGGT GGCGGATCAG 
ATCCCACAGC TGGTTCAGGG TCTGAGGAGC AGCCAGGCTC AACCTGACAG CCCCAGCGCT 
CAACTCGCCC TCATCGGAGC TAGCCAGAAC TTCCTGCAGC CCGGAGCCAA AATGGTGACC 
GCAGCCAAAG CTACAGTTCC AACCATCAGT GACCAGGCGT CTGCCATGCA GCTCAGTCAG 
TGCGCTAAAA ACCTGGCCAG TGCTCTGGCT GAGCTCCGCA CGGCCGCCCA GAAGGCTCAG 
GAGGCGTGTG GTCCGTTGGA GATTGATAAC GCTCTGACTA TGGTACGGGG GCTGGAGAGG 
GACATGCAGG AGGCCAAAGC TTCAGCTGCT GAGGGCAAAC TCAGACCCCT GCCTGGAGAA 
ACGCTGGAGA AGTGCTCTCA GGATCTGGGC TCCAGCACTA AAGCCGTGAG CTCTGCCATT 
GCTCAGCTCC TCAGTGAAGC AACTCAAGGC AATGAGAACT ACACAGGTAT GGCTGCTCGT 
GATGTGGCTC AGGCTCTGCG CTCGCTGGCT TCAGCCGCTC GTGGAGTGGC AGCCAACACT 
GAAGATCCTC TAGCCCGTAA CGCAATGCTG GACTGCACCG GCGATGTCAT GGACAAATCC 
GCCAGCCTGA TTGAAGAGAC CAAGAGGGCC ATAGCCAAAC CAGGAGACCC TGACAGTCAG 
CAAAGACTGG CCCAGGTAGC CAAGGCTGTG TCTCAGGCAC TGAACCGCTC TGTAAACTGT 
CTGCCGGGGC AGAGGGATGT GGATAATGCC ATCAGAACTG TCGGGGAAGC TAGCAAGAAA 
CTGCTGTCTG ATAAATTTCC AGCGAGTGGG AAGAATTTCC AAGAGGCTCA GGCCAATCTG 
AACGAGGCTG CAGCTGGACT AAACCAGTCA GCCAATGAGC TGGTGCAGGC CTCGCGTGGC 
ACCACCCAGG ACCTGGCCAA AGCCTCCGGG AAGTTTGGAC AAGACTTTAA CCAATTCCTG 
CAGGCTGGAG TGGACATGGC TGGACAATCC CAGTCTAAAG AAGACCAAAC TCAAGTGGTC 
ACCAACCTGA AGACCATCTC CATGTCCTCC AGCAAACTCC TGCTGGCGGC TAAAGCTCTG 
TCCACAGACC CCAACTCGCC AAATCTGAAG AACCAACTCG CTGCTGCTGC TCGCGCGGTG 
ACAGACAGCA TTAATCAGCT GATCAGCATG TGTACCCAGC AGGCTCCGGG ACAGAAGGAG 
TGTGACAACG CTCTCCGTGA GCTTGAGACG GTTGGAGGAA TGCTGGAGAA CCCAACAGAA 
GCAGTCAATG ATTTGGGCTA CTTTGACTGC ATTGACAGTG TGATGGAGAA CTCAAAGGTT 
TTGGGTGAGG CCATGGCTGG CATTTCCCAT AATGCCAAAA ACAGTAACTT GCCAGAGTTT 
GGAGATTCGG TCAGTTCAGC GTCTAAAGCG CTGTGTGGTC TCACTGAAGC AGCTGCTCAG 
GGGGCGTATC TGGTTGGTGT GTCAGACCCG AACAGCCATG CTGGCCAGAA GGGTCTTGTG 
GACCCCTCAC AATTTGCCAG AGCCAAACAG TCCATCCAGA TGGCCTGTCA GAACCTGGTG 
GATCCAGCCT GCACCCAGTC CCAGGTTCTC TCTGCTGCCA CCATCGTTGC CAAACACACT 
TCGGCCTTGT GCAATGCCTG CCGGCTCGCC TCCTCCAAGA CATCCAACCC AGTTGCTAAG 
AGACAGTTCG TCCAGAGTGC CAAAGAGGTG GCCAACAGCA CAGCCAACCT CGTCAAGTCC 
ATCAAGGCTC TCGACGGGGC CTTTAACCAG GAAAACAGAC AGAAGTGTAA GGAAGCCACT 
GGGCCACTCA TTGAGGCTGT GGATAACCTG ACTGCCTTCG CTTCAAACCC AGAGTTCGCA 
AGTGTACCCG CTCAGATCAG CCCAGAGGGT CTGGCTGCGA TGGAGCCGAT CACAGCAGCC 
GCTAAGGCAA TGTTGGAAAG CTCGACAGGA CTGATCCAGA CGGCTCGCTC GCTCGCAGTC 
AACCCGAAAG ATCCACCCAA GTGGTCTGTG CTTGCTGGAC ACTCCAGAAC AGTGTCCGAC 
TCCATCAAGA AACTCATCAC TAACATGAGG GAAAAGGCTC CGGGTCAGAG GGAGTGTGAT 
GATGCCATTG AGGTTCTTAG TAACTGCATT CGTGAAGTGG ACAAGGCCTC GCTGGCAGCC 
ATCAGTCAGC AGCTCACGCC TAGAGACGAC ATTTCCCATG AGGCTCTGCA CGAGCAGATG 
GCGGCGTCTG TGCATGAGAT CAGTAACCTG ATTGAACCAG TGGCCATCGC AGCCCGTGCT 
GATGCCTCTC AGCTGGGACA CAAGGTGTCT CAGATGGTCA GTTACTTTGA ACCGCTCATC 
ATGGCTGCCA TCGGCACAGC GTCTAAGATC CTGAACAGCC AGCAGCAGAT GAACGTGCTG 
GACCAAACCA AGACTCTGGC TGAGTCTGCG CTACAGATGC TCTATACGGC CAAAGAGACT 
GGAGGAAACC ACAAGGCGGC GCACACACAA GAGGCTCTGG AGGAGTCTGT TCAGATGATG 
AAGGAGGCGG TGGACGATCT GGGTTCGACT CTGGCAGAAG TGGCCAGCGC TGCAGGAGCA 
GTGGGAGGAA TGGTCAACTC CATCACTCAG TCCATCAACA AGATGGAGGA CGGACCTGCC 
TTCGAGCCTG AAGGAACGTT TGTGGATTAT CAGACCACCA TGGTGAAGAC AGCCAAAGCT 
ATCGCTGTCA CCGTTCAGGA GATGGTTACC AAATCCAACA CCAACCCTGA TGAGCTGGGC 
GGTTTGGCCA ATCAGCTGAC CACAGAGTTC GGTGACCTCG CATCAGAGGC CAAATGTGCA 
GCCATGACTG CTGAAAACGA TGAGATCGGT TCACATATCA AGAAGCAGGT GACTGAGTTG 
GGCTACACCT GCACTGGTCT GGTCAGTAAA GCAGGAGCGC TACAGTGCAG TCCAAACGAC 
TCCTACACTA AGAAGGAGCT CATTGACAGT GCCAGGAAAG TCTCTGAGAA GGTCTCTCAT 
GTGCTGGCAG CTCTTCAGGC AGGCAATCGT GGCACTCAGG CCTGCATTAC TGCCGCGAGC 
GCTGTCTCTG GCATCATTGC TGATTTAGAC ACCACCATCA TGTTTGCCAC CGCGGGCACT 
CTCAACCGAG AGAACGCAGA GACATTTGCA GACCACAGAG AGCACATCTT AAAGACGGCT 
AAAGTTCTGG TGGAGGACAC GAAGCTGCTG GTTTCTGGAG CCGGAGCAAG TCAGGAGAAA 
CTGGCTCAGG CTGCACAGTC CTCCGTCAAC ACCATCACCA AACTGGCCGA TGTGGTTAAA 
TTGGGGGCAG CGAGTCTGGG CTCTGAAGAC CCAGAGACAC AGGTGGTTCT GATCAACGCT 
GTGAAGGACG TGGCTAAAGC TCTGGGTGAC CTGATCAGCG CCACCAAAGC AGCGGCCGGC 
AAACCTCACG ATGACCCCGC CATGCTGCAG CTCAAAAACT CTGCTAAGGT GATGGTGACC 
AATGTGACGT CTCTACTTAA GACGGTGAAG GCGGTGGAGG ATGAAGCCAC TAAAGGCACA 
CGAGCACTAG AGGCCACCAT AGAGCACATC AAACAAGAGC TGGCTGTTTT CAGCAGTGCA 
GATCCTCCTC CAAAGACGGC CACTCCAGAG GAGTTCATCC GCATGACCAA AGGCATCACA 
TTGGCCACAG CTAAAGCAGT GGCTGCTGGG AACTCCTGCC GTCAGGAGGA CGTGATCGCC 
ACTGCTAACC TAAGCAGACG AGCTGTCGCA GACATGCTGC GCTCCTGCAA GCAAGCCGCA 
TACCACCCGG AGGTGAAGCG GGAGGTCCAG ACTCGAGCGC TCCGCTTTGG CTCTGAGTGC 
TCCACAAGTT ACCTGGGCCT GCTGGAGCAC GTGTTAGTGA TCATTCAGAA ACCCTCGCAT 
GACCTTAAAC AGCAGCTGGC AAACTACTCC AAACGTGTGG CCAGTTCAGT GACCGAGCTC 
ATCCAGGCTG CGGAGGCCAT GAAAGGTACG GAGTGGGTGG ATCCCGAAGA CCCCACCGTC 
ATTGCAGAAA ACGAGCTGCT GGGAGCCGCA GCTGCCATTG AAGCTGCCGC GAAGAAACTA 
GAGCAGCTCA AACCCCGAGC CAAACCCAAG GAAGCTGATG AGAGTCTGAA CTTTGAGGAA 
CAGATTCTAG AAGCGGCCAA ATCCATCGCT GCAGCCACCA GTGCTCTGGT CAAAGCTGCG 
TCTGCCGCTC AGAGAGAGCT GGTGGCTCAA GGGAAGGTTG GGGCAATTCC AGCAAACGCG 
GTGGATGATG GACAGTGGTC TCAGGGTCTC ATTTCAGCTG CTCGTATGGT AGCCGCAGCT 
ACTAACAACC TGTGCGAAGC AGCTAACTCT GCGGTTCAGG GTCACGCCAG CGAGGAGAAA 
CTCATTTCTT CAGCCAAACA GGTGGCAGCA TCCACCGCAC AGCTGCTGGT GGCCTGTAAG 
GTCAAGGCTG ACCAGGACTC CCAGACCATG AAGAGACTGC AGGCTGCTGG TAATGCTGTC 
AAGAGGGCGT CTGATAACCT GGTGAAGGCG GCACAGAAGG CTGCGTTTGA GGCTCAAGAT 
GATCAGGCTG TGATGGTGAA GAGCAAGATG GTGGGCGGTA TTGCTCAGAT CATCGCTGCT 
CAGGAGGAGA TGCTCAGGAA GGAGAAAGAG CTGGAAGAAG CCAGAAAGAA ACTGGCAATG 
ATCCGACAGC AGCAGTACAA GTTCCTGCCC TCAGAGCTGC GAGAGGACGG ACAGGAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155556.1
CDS	202..7884
Translation	MVALSLKIGVGNVVKTMQFEPSTVVYDACRIIRERVPEAQLGQPSDYGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTLEYKKKQRPLKIRMLDGTVKTVMVDDSKIVSDMLMTICARIGITNYDEYSLVRDVGEEKKEETTGTLKRDKTLLRDDKKMEKLKQKLHTDDELNWLDHGRTLREQGVEESEMLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGYQCQIQFGDHNESKHKPGFLDLKEFLPKEYVKNKGEKRIFQAHKNCQNMTEIEAKVNYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKESVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQFNKMGKVETGSVALPAIIRSGAGGPESMKMGSMPQAKQHITSGQMHRGHMPPLTSAQQALTGTINSSMQAVQAVQASLDEFDALPPLGTDSASQAWRKNKMDESKHEIHSQVDAITAGTASMVNLTAGDPAETDYTAVGCAVTTISSNLTEMSKGVKLLAALMEDEGGNGQQLLGAAKNLACAVSDMLKTAQPSSTEPRQNLLQAAGNVGQASGELLQQIGESDADQHFQSVGTAAFTHSRRTGYSQMLDMLMQLAKAVANAAAALVLKAKNVAQKSEDSAQQNKVIAAATQCALSTSQLVACTRVVAPTISSPVCQEQLIEAGKLVAKSVEGCVEASHGATGDEHLLKQVGAAATGVTQALNELLQHIRQYASGGQPIGRHGEATDRILDVTDNIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGESDLENSRKLLSAAKLLADATARMVEAAKGAAANPDSEEQQQKLREAAEGLRMATNAAAQNAIKKRLVNKLENAAKQAAAAATQTIAAAQHAASSNKNPAAQQQLVQSCKVVADQIPQLVQGLRSSQAQPDSPSAQLALIGASQNFLQPGAKMVTAAKATVPTISDQASAMQLSQCAKNLASALAELRTAAQKAQEACGPLEIDNALTMVRGLERDMQEAKASAAEGKLRPLPGETLEKCSQDLGSSTKAVSSAIAQLLSEATQGNENYTGMAARDVAQALRSLASAARGVAANTEDPLARNAMLDCTGDVMDKSASLIEETKRAIAKPGDPDSQQRLAQVAKAVSQALNRSVNCLPGQRDVDNAIRTVGEASKKLLSDKFPASGKNFQEAQANLNEAAAGLNQSANELVQASRGTTQDLAKASGKFGQDFNQFLQAGVDMAGQSQSKEDQTQVVTNLKTISMSSSKLLLAAKALSTDPNSPNLKNQLAAAARAVTDSINQLISMCTQQAPGQKECDNALRELETVGGMLENPTEAVNDLGYFDCIDSVMENSKVLGEAMAGISHNAKNSNLPEFGDSVSSASKALCGLTEAAAQGAYLVGVSDPNSHAGQKGLVDPSQFARAKQSIQMACQNLVDPACTQSQVLSAATIVAKHTSALCNACRLASSKTSNPVAKRQFVQSAKEVANSTANLVKSIKALDGAFNQENRQKCKEATGPLIEAVDNLTAFASNPEFASVPAQISPEGLAAMEPITAAAKAMLESSTGLIQTARSLAVNPKDPPKWSVLAGHSRTVSDSIKKLITNMREKAPGQRECDDAIEVLSNCIREVDKASLAAISQQLTPRDDISHEALHEQMAASVHEISNLIEPVAIAARADASQLGHKVSQMVSYFEPLIMAAIGTASKILNSQQQMNVLDQTKTLAESALQMLYTAKETGGNHKAAHTQEALEESVQMMKEAVDDLGSTLAEVASAAGAVGGMVNSITQSINKMEDGPAFEPEGTFVDYQTTMVKTAKAIAVTVQEMVTKSNTNPDELGGLANQLTTEFGDLASEAKCAAMTAENDEIGSHIKKQVTELGYTCTGLVSKAGALQCSPNDSYTKKELIDSARKVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNRENAETFADHREHILKTAKVLVEDTKLLVSGAGASQEKLAQAAQSSVNTITKLADVVKLGAASLGSEDPETQVVLINAVKDVAKALGDLISATKAAAGKPHDDPAMLQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATIEHIKQELAVFSSADPPPKTATPEEFIRMTKGITLATAKAVAAGNSCRQEDVIATANLSRRAVADMLRSCKQAAYHPEVKREVQTRALRFGSECSTSYLGLLEHVLVIIQKPSHDLKQQLANYSKRVASSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATNNLCEAANSAVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSQTMKRLQAAGNAVKRASDNLVKAAQKAAFEAQDDQAVMVKSKMVGGIAQIIAAQEEMLRKEKELEEARKKLAMIRQQQYKFLPSELREDGQEQ

Target ORF information:

RefSeq Version	XM_005155499.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio talin 1 (tln1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155499.4

ORF Insert Sequence:

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGTCTGT AGGTACAGCA GCGTTCACAC ACTCCAGACG GACAGGCTAT 
AGTCAAATGC TGGACATGTT GATGCAGTTG GCGAAGGCTG TGGCGAATGC AGCAGCGGCT 
CTGGTGTTAA AGGCTAAAAA CGTGGCTCAG AAAAGCGAAG ATTCAGCTCA ACAAAACAAA 
GTGATCGCTG CTGCCACCCA GTGCGCCCTC TCCACATCAC AGCTGGTGGC CTGCACACGA 
GTGGTGGCTC CAACCATCAG CTCACCCGTG TGTCAAGAGC AGCTCATCGA GGCTGGAAAA 
CTGGTGGCGA AGTCTGTGGA GGGCTGTGTG GAGGCGTCAC ACGGGGCGAC CGGTGACGAG 
CATCTGCTCA AACAGGTGGG CGCCGCTGCC ACAGGAGTCA CTCAGGCTCT TAACGAGCTG 
CTGCAGCATA TTAGACAGTA CGCCAGCGGA GGACAGCCAA TCGGACGGCA CGGAGAGGCT 
ACCGACCGCA TCCTGGATGT GACTGATAAC ATCTTCAGCT CCATGGGAGA TGCAGGGGAG 
ATGGTGCGTC AGGCGCGGAT CCTTGCTCAG GCCACCTCAG ACCTGGTCAA CGCGATCAAG 
GCGGACGCAG AGGGAGAATC TGACCTGGAG AACTCTCGCA AACTCCTCTC TGCAGCCAAA 
CTCCTGGCTG ACGCTACTGC CCGTATGGTG GAAGCTGCGA AGGGTGCAGC TGCTAATCCA 
GACAGCGAGG AGCAGCAGCA GAAGTTGCGT GAAGCTGCAG AGGGACTGCG CATGGCCACC 
AATGCTGCAG CACAGAACGC CATTAAAAAG AGACTCGTCA ACAAACTGGA GAATGCAGCG 
AAACAAGCCG CAGCAGCCGC CACTCAGACC ATAGCAGCCG CTCAACATGC AGCTTCATCC 
AACAAGAACC CTGCGGCCCA GCAACAACTC GTACAGAGCT GCAAGGTGGT GGCGGATCAG 
ATCCCACAGC TGGTTCAGGG TCTGAGGAGC AGCCAGGCTC AACCTGACAG CCCCAGCGCT 
CAACTCGCCC TCATCGGAGC TAGCCAGAAC TTCCTGCAGC CCGGAGCCAA AATGGTGACC 
GCAGCCAAAG CTACAGTTCC AACCATCAGT GACCAGGCGT CTGCCATGCA GCTCAGTCAG 
TGCGCTAAAA ACCTGGCCAG TGCTCTGGCT GAGCTCCGCA CGGCCGCCCA GAAGGCTCAG 
GAGGCGTGTG GTCCGTTGGA GATTGATAAC GCTCTGACTA TGGTACGGGG GCTGGAGAGG 
GACATGCAGG AGGCCAAAGC TTCAGCTGCT GAGGGCAAAC TCAGACCCCT GCCTGGAGAA 
ACGCTGGAGA AGTGCTCTCA GGATCTGGGC TCCAGCACTA AAGCCGTGAG CTCTGCCATT 
GCTCAGCTCC TCAGTGAAGC AACTCAAGGC AATGAGAACT ACACAGGTAT GGCTGCTCGT 
GATGTGGCTC AGGCTCTGCG CTCGCTGGCT TCAGCCGCTC GTGGAGTGGC AGCCAACACT 
GAAGATCCTC TAGCCCGTAA CGCAATGCTG GACTGCACCG GCGATGTCAT GGACAAATCC 
GCCAGCCTGA TTGAAGAGAC CAAGAGGGCC ATAGCCAAAC CAGGAGACCC TGACAGTCAG 
CAAAGACTGG CCCAGGTAGC CAAGGCTGTG TCTCAGGCAC TGAACCGCTC TGTAAACTGT 
CTGCCGGGGC AGAGGGATGT GGATAATGCC ATCAGAACTG TCGGGGAAGC TAGCAAGAAA 
CTGCTGTCTG ATAAATTTCC AGCGAGTGGG AAGAATTTCC AAGAGGCTCA GGCCAATCTG 
AACGAGGCTG CAGCTGGACT AAACCAGTCA GCCAATGAGC TGGTGCAGGC CTCGCGTGGC 
ACCACCCAGG ACCTGGCCAA AGCCTCCGGG AAGTTTGGAC AAGACTTTAA CCAATTCCTG 
CAGGCTGGAG TGGACATGGC TGGACAATCC CAGTCTAAAG AAGACCAAAC TCAAGTGGTC 
ACCAACCTGA AGACCATCTC CATGTCCTCC AGCAAACTCC TGCTGGCGGC TAAAGCTCTG 
TCCACAGACC CCAACTCGCC AAATCTGAAG AACCAACTCG CTGCTGCTGC TCGCGCGGTG 
ACAGACAGCA TTAATCAGCT GATCAGCATG TGTACCCAGC AGGCTCCGGG ACAGAAGGAG 
TGTGACAACG CTCTCCGTGA GCTTGAGACG GTTGGAGGAA TGCTGGAGAA CCCAACAGAA 
GCAGTCAATG ATTTGGGCTA CTTTGACTGC ATTGACAGTG TGATGGAGAA CTCAAAGGTT 
TTGGGTGAGG CCATGGCTGG CATTTCCCAT AATGCCAAAA ACAGTAACTT GCCAGAGTTT 
GGAGATTCGG TCAGTTCAGC GTCTAAAGCG CTGTGTGGTC TCACTGAAGC AGCTGCTCAG 
GGGGCGTATC TGGTTGGTGT GTCAGACCCG AACAGCCATG CTGGCCAGAA GGGTCTTGTG 
GACCCCTCAC AATTTGCCAG AGCCAAACAG TCCATCCAGA TGGCCTGTCA GAACCTGGTG 
GATCCAGCCT GCACCCAGTC CCAGGTTCTC TCTGCTGCCA CCATCGTTGC CAAACACACT 
TCGGCCTTGT GCAATGCCTG CCGGCTCGCC TCCTCCAAGA CATCCAACCC AGTTGCTAAG 
AGACAGTTCG TCCAGAGTGC CAAAGAGGTG GCCAACAGCA CAGCCAACCT CGTCAAGTCC 
ATCAAGGCTC TCGACGGGGC CTTTAACCAG GAAAACAGAC AGAAGTGTAA GGAAGCCACT 
GGGCCACTCA TTGAGGCTGT GGATAACCTG ACTGCCTTCG CTTCAAACCC AGAGTTCGCA 
AGTGTACCCG CTCAGATCAG CCCAGAGGGT CTGGCTGCGA TGGAGCCGAT CACAGCAGCC 
GCTAAGGCAA TGTTGGAAAG CTCGACAGGA CTGATCCAGA CGGCTCGCTC GCTCGCAGTC 
AACCCGAAAG ATCCACCCAA GTGGTCTGTG CTTGCTGGAC ACTCCAGAAC AGTGTCCGAC 
TCCATCAAGA AACTCATCAC TAACATGAGG GAAAAGGCTC CGGGTCAGAG GGAGTGTGAT 
GATGCCATTG AGGTTCTTAG TAACTGCATT CGTGAAGTGG ACAAGGCCTC GCTGGCAGCC 
ATCAGTCAGC AGCTCACGCC TAGAGACGAC ATTTCCCATG AGGCTCTGCA CGAGCAGATG 
GCGGCGTCTG TGCATGAGAT CAGTAACCTG ATTGAACCAG TGGCCATCGC AGCCCGTGCT 
GATGCCTCTC AGCTGGGACA CAAGGTGTCT CAGATGGTCA GTTACTTTGA ACCGCTCATC 
ATGGCTGCCA TCGGCACAGC GTCTAAGATC CTGAACAGCC AGCAGCAGAT GAACGTGCTG 
GACCAAACCA AGACTCTGGC TGAGTCTGCG CTACAGATGC TCTATACGGC CAAAGAGACT 
GGAGGAAACC ACAAGGCGGC GCACACACAA GAGGCTCTGG AGGAGTCTGT TCAGATGATG 
AAGGAGGCGG TGGACGATCT GGGTTCGACT CTGGCAGAAG TGGCCAGCGC TGCAGGAGCA 
GTGGGAGGAA TGGTCAACTC CATCACTCAG TCCATCAACA AGATGGAGGA CGGACCTGCC 
TTCGAGCCTG AAGGAACGTT TGTGGATTAT CAGACCACCA TGGTGAAGAC AGCCAAAGCT 
ATCGCTGTCA CCGTTCAGGA GATGGTTACC AAATCCAACA CCAACCCTGA TGAGCTGGGC 
GGTTTGGCCA ATCAGCTGAC CACAGAGTTC GGTGACCTCG CATCAGAGGC CAAATGTGCA 
GCCATGACTG CTGAAAACGA TGAGATCGGT TCACATATCA AGAAGCAGGT GACTGAGTTG 
GGCTACACCT GCACTGGTCT GGTCAGTAAA GCAGGAGCGC TACAGTGCAG TCCAAACGAC 
TCCTACACTA AGAAGGAGCT CATTGACAGT GCCAGGAAAG TCTCTGAGAA GGTCTCTCAT 
GTGCTGGCAG CTCTTCAGGC AGGCAATCGT GGCACTCAGG CCTGCATTAC TGCCGCGAGC 
GCTGTCTCTG GCATCATTGC TGATTTAGAC ACCACCATCA TGTTTGCCAC CGCGGGCACT 
CTCAACCGAG AGAACGCAGA GACATTTGCA GACCACAGAG AGCACATCTT AAAGACGGCT 
AAAGTTCTGG TGGAGGACAC GAAGCTGCTG GTTTCTGGAG CCGGAGCAAG TCAGGAGAAA 
CTGGCTCAGG CTGCACAGTC CTCCGTCAAC ACCATCACCA AACTGGCCGA TGTGGTTAAA 
TTGGGGGCAG CGAGTCTGGG CTCTGAAGAC CCAGAGACAC AGGTGGTTCT GATCAACGCT 
GTGAAGGACG TGGCTAAAGC TCTGGGTGAC CTGATCAGCG CCACCAAAGC AGCGGCCGGC 
AAACCTCACG ATGACCCCGC CATGCTGCAG CTCAAAAACT CTGCTAAGGT GATGGTGACC 
AATGTGACGT CTCTACTTAA GACGGTGAAG GCGGTGGAGG ATGAAGCCAC TAAAGGCACA 
CGAGCACTAG AGGCCACCAT AGAGCACATC AAACAAGAGC TGGCTGTTTT CAGCAGTGCA 
GATCCTCCTC CAAAGACGGC CACTCCAGAG GAGTTCATCC GCATGACCAA AGGCATCACA 
TTGGCCACAG CTAAAGCAGT GGCTGCTGGG AACTCCTGCC GTCAGGAGGA CGTGATCGCC 
ACTGCTAACC TAAGCAGACG AGCTGTCGCA GACATGCTGC GCTCCTGCAA GCAAGCCGCA 
TACCACCCGG AGGTGAAGCG GGAGGTCCAG ACTCGAGCGC TCCGCTTTGG CTCTGAGTGC 
TCCACAAGTT ACCTGGGCCT GCTGGAGCAC GTGTTAGTGA TCATTCAGAA ACCCTCGCAT 
GACCTTAAAC AGCAGCTGGC AAACTACTCC AAACGTGTGG CCAGTTCAGT GACCGAGCTC 
ATCCAGGCTG CGGAGGCCAT GAAAGGTACG GAGTGGGTGG ATCCCGAAGA CCCCACCGTC 
ATTGCAGAAA ACGAGCTGCT GGGAGCCGCA GCTGCCATTG AAGCTGCCGC GAAGAAACTA 
GAGCAGCTCA AACCCCGAGC CAAACCCAAG GAAGCTGATG AGAGTCTGAA CTTTGAGGAA 
CAGATTCTAG AAGCGGCCAA ATCCATCGCT GCAGCCACCA GTGCTCTGGT CAAAGCTGCG 
TCTGCCGCTC AGAGAGAGCT GGTGGCTCAA GGGAAGGTTG GGGCAATTCC AGCAAACGCG 
GTGGATGATG GACAGTGGTC TCAGGGTCTC ATTTCAGCTG CTCGTATGGT AGCCGCAGCT 
ACTAACAACC TGTGCGAAGC AGCTAACTCT GCGGTTCAGG GTCACGCCAG CGAGGAGAAA 
CTCATTTCTT CAGCCAAACA GGTGGCAGCA TCCACCGCAC AGCTGCTGGT GGCCTGTAAG 
GTCAAGGCTG ACCAGGACTC CCAGACCATG AAGAGACTGC AGGCTGCTGG TAATGCTGTC 
AAGAGGGCGT CTGATAACCT GGTGAAGGCG GCACAGAAGG CTGCGTTTGA GGCTCAAGAT 
GATCAGGCTG TGATGGTGAA GAGCAAGATG GTGGGCGGTA TTGCTCAGAT CATCGCTGCT 
CAGGAGGAGA TGCTCAGGAA GGAGAAAGAG CTGGAAGAAG CCAGAAAGAA ACTGGCAATG 
ATCCGACAGC AGCAGTACAA GTTCCTGCCC TCAGAGCTGC GAGAGGACGG ACAGGAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tln1 ( XM_005155500.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155557.1 Danio rerio talin 1 (tln1), transcript variant X4, mRNA.

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CloneID	ODa15806
Clone ID Related Accession (Same CDS sequence)	XM_005155500.4 , XM_005155500.3
Accession Version	XM_005155500.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	talin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155500.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGGACAT GTTGATGCAG TTGGCGAAGG CTGTGGCGAA TGCAGCAGCG 
GCTCTGGTGT TAAAGGCTAA AAACGTGGCT CAGAAAAGCG AAGATTCAGC TCAACAAAAC 
AAAGTGATCG CTGCTGCCAC CCAGTGCGCC CTCTCCACAT CACAGCTGGT GGCCTGCACA 
CGAGTGGTGG CTCCAACCAT CAGCTCACCC GTGTGTCAAG AGCAGCTCAT CGAGGCTGGA 
AAACTGGTGG CGAAGTCTGT GGAGGGCTGT GTGGAGGCGT CACACGGGGC GACCGGTGAC 
GAGCATCTGC TCAAACAGGT GGGCGCCGCT GCCACAGGAG TCACTCAGGC TCTTAACGAG 
CTGCTGCAGC ATATTAGACA GTACGCCAGC GGAGGACAGC CAATCGGACG GCACGGAGAG 
GCTACCGACC GCATCCTGGA TGTGACTGAT AACATCTTCA GCTCCATGGG AGATGCAGGG 
GAGATGGTGC GTCAGGCGCG GATCCTTGCT CAGGCCACCT CAGACCTGGT CAACGCGATC 
AAGGCGGACG CAGAGGGAGA ATCTGACCTG GAGAACTCTC GCAAACTCCT CTCTGCAGCC 
AAACTCCTGG CTGACGCTAC TGCCCGTATG GTGGAAGCTG CGAAGGGTGC AGCTGCTAAT 
CCAGACAGCG AGGAGCAGCA GCAGAAGTTG CGTGAAGCTG CAGAGGGACT GCGCATGGCC 
ACCAATGCTG CAGCACAGAA CGCCATTAAA AAGAGACTCG TCAACAAACT GGAGAATGCA 
GCGAAACAAG CCGCAGCAGC CGCCACTCAG ACCATAGCAG CCGCTCAACA TGCAGCTTCA 
TCCAACAAGA ACCCTGCGGC CCAGCAACAA CTCGTACAGA GCTGCAAGGT GGTGGCGGAT 
CAGATCCCAC AGCTGGTTCA GGGTCTGAGG AGCAGCCAGG CTCAACCTGA CAGCCCCAGC 
GCTCAACTCG CCCTCATCGG AGCTAGCCAG AACTTCCTGC AGCCCGGAGC CAAAATGGTG 
ACCGCAGCCA AAGCTACAGT TCCAACCATC AGTGACCAGG CGTCTGCCAT GCAGCTCAGT 
CAGTGCGCTA AAAACCTGGC CAGTGCTCTG GCTGAGCTCC GCACGGCCGC CCAGAAGGCT 
CAGGAGGCGT GTGGTCCGTT GGAGATTGAT AACGCTCTGA CTATGGTACG GGGGCTGGAG 
AGGGACATGC AGGAGGCCAA AGCTTCAGCT GCTGAGGGCA AACTCAGACC CCTGCCTGGA 
GAAACGCTGG AGAAGTGCTC TCAGGATCTG GGCTCCAGCA CTAAAGCCGT GAGCTCTGCC 
ATTGCTCAGC TCCTCAGTGA AGCAACTCAA GGCAATGAGA ACTACACAGG TATGGCTGCT 
CGTGATGTGG CTCAGGCTCT GCGCTCGCTG GCTTCAGCCG CTCGTGGAGT GGCAGCCAAC 
ACTGAAGATC CTCTAGCCCG TAACGCAATG CTGGACTGCA CCGGCGATGT CATGGACAAA 
TCCGCCAGCC TGATTGAAGA GACCAAGAGG GCCATAGCCA AACCAGGAGA CCCTGACAGT 
CAGCAAAGAC TGGCCCAGGT AGCCAAGGCT GTGTCTCAGG CACTGAACCG CTCTGTAAAC 
TGTCTGCCGG GGCAGAGGGA TGTGGATAAT GCCATCAGAA CTGTCGGGGA AGCTAGCAAG 
AAACTGCTGT CTGATAAATT TCCAGCGAGT GGGAAGAATT TCCAAGAGGC TCAGGCCAAT 
CTGAACGAGG CTGCAGCTGG ACTAAACCAG TCAGCCAATG AGCTGGTGCA GGCCTCGCGT 
GGCACCACCC AGGACCTGGC CAAAGCCTCC GGGAAGTTTG GACAAGACTT TAACCAATTC 
CTGCAGGCTG GAGTGGACAT GGCTGGACAA TCCCAGTCTA AAGAAGACCA AACTCAAGTG 
GTCACCAACC TGAAGACCAT CTCCATGTCC TCCAGCAAAC TCCTGCTGGC GGCTAAAGCT 
CTGTCCACAG ACCCCAACTC GCCAAATCTG AAGAACCAAC TCGCTGCTGC TGCTCGCGCG 
GTGACAGACA GCATTAATCA GCTGATCAGC ATGTGTACCC AGCAGGCTCC GGGACAGAAG 
GAGTGTGACA ACGCTCTCCG TGAGCTTGAG ACGGTTGGAG GAATGCTGGA GAACCCAACA 
GAAGCAGTCA ATGATTTGGG CTACTTTGAC TGCATTGACA GTGTGATGGA GAACTCAAAG 
GTTTTGGGTG AGGCCATGGC TGGCATTTCC CATAATGCCA AAAACAGTAA CTTGCCAGAG 
TTTGGAGATT CGGTCAGTTC AGCGTCTAAA GCGCTGTGTG GTCTCACTGA AGCAGCTGCT 
CAGGGGGCGT ATCTGGTTGG TGTGTCAGAC CCGAACAGCC ATGCTGGCCA GAAGGGTCTT 
GTGGACCCCT CACAATTTGC CAGAGCCAAA CAGTCCATCC AGATGGCCTG TCAGAACCTG 
GTGGATCCAG CCTGCACCCA GTCCCAGGTT CTCTCTGCTG CCACCATCGT TGCCAAACAC 
ACTTCGGCCT TGTGCAATGC CTGCCGGCTC GCCTCCTCCA AGACATCCAA CCCAGTTGCT 
AAGAGACAGT TCGTCCAGAG TGCCAAAGAG GTGGCCAACA GCACAGCCAA CCTCGTCAAG 
TCCATCAAGG CTCTCGACGG GGCCTTTAAC CAGGAAAACA GACAGAAGTG TAAGGAAGCC 
ACTGGGCCAC TCATTGAGGC TGTGGATAAC CTGACTGCCT TCGCTTCAAA CCCAGAGTTC 
GCAAGTGTAC CCGCTCAGAT CAGCCCAGAG GGTCTGGCTG CGATGGAGCC GATCACAGCA 
GCCGCTAAGG CAATGTTGGA AAGCTCGACA GGACTGATCC AGACGGCTCG CTCGCTCGCA 
GTCAACCCGA AAGATCCACC CAAGTGGTCT GTGCTTGCTG GACACTCCAG AACAGTGTCC 
GACTCCATCA AGAAACTCAT CACTAACATG AGGGAAAAGG CTCCGGGTCA GAGGGAGTGT 
GATGATGCCA TTGAGGTTCT TAGTAACTGC ATTCGTGAAG TGGACAAGGC CTCGCTGGCA 
GCCATCAGTC AGCAGCTCAC GCCTAGAGAC GACATTTCCC ATGAGGCTCT GCACGAGCAG 
ATGGCGGCGT CTGTGCATGA GATCAGTAAC CTGATTGAAC CAGTGGCCAT CGCAGCCCGT 
GCTGATGCCT CTCAGCTGGG ACACAAGGTG TCTCAGATGG TCAGTTACTT TGAACCGCTC 
ATCATGGCTG CCATCGGCAC AGCGTCTAAG ATCCTGAACA GCCAGCAGCA GATGAACGTG 
CTGGACCAAA CCAAGACTCT GGCTGAGTCT GCGCTACAGA TGCTCTATAC GGCCAAAGAG 
ACTGGAGGAA ACCACAAGGC GGCGCACACA CAAGAGGCTC TGGAGGAGTC TGTTCAGATG 
ATGAAGGAGG CGGTGGACGA TCTGGGTTCG ACTCTGGCAG AAGTGGCCAG CGCTGCAGGA 
GCAGTGGGAG GAATGGTCAA CTCCATCACT CAGTCCATCA ACAAGATGGA GGACGGACCT 
GCCTTCGAGC CTGAAGGAAC GTTTGTGGAT TATCAGACCA CCATGGTGAA GACAGCCAAA 
GCTATCGCTG TCACCGTTCA GGAGATGGTT ACCAAATCCA ACACCAACCC TGATGAGCTG 
GGCGGTTTGG CCAATCAGCT GACCACAGAG TTCGGTGACC TCGCATCAGA GGCCAAATGT 
GCAGCCATGA CTGCTGAAAA CGATGAGATC GGTTCACATA TCAAGAAGCA GGTGACTGAG 
TTGGGCTACA CCTGCACTGG TCTGGTCAGT AAAGCAGGAG CGCTACAGTG CAGTCCAAAC 
GACTCCTACA CTAAGAAGGA GCTCATTGAC AGTGCCAGGA AAGTCTCTGA GAAGGTCTCT 
CATGTGCTGG CAGCTCTTCA GGCAGGCAAT CGTGGCACTC AGGCCTGCAT TACTGCCGCG 
AGCGCTGTCT CTGGCATCAT TGCTGATTTA GACACCACCA TCATGTTTGC CACCGCGGGC 
ACTCTCAACC GAGAGAACGC AGAGACATTT GCAGACCACA GAGAGCACAT CTTAAAGACG 
GCTAAAGTTC TGGTGGAGGA CACGAAGCTG CTGGTTTCTG GAGCCGGAGC AAGTCAGGAG 
AAACTGGCTC AGGCTGCACA GTCCTCCGTC AACACCATCA CCAAACTGGC CGATGTGGTT 
AAATTGGGGG CAGCGAGTCT GGGCTCTGAA GACCCAGAGA CACAGGTGGT TCTGATCAAC 
GCTGTGAAGG ACGTGGCTAA AGCTCTGGGT GACCTGATCA GCGCCACCAA AGCAGCGGCC 
GGCAAACCTC ACGATGACCC CGCCATGCTG CAGCTCAAAA ACTCTGCTAA GGTGATGGTG 
ACCAATGTGA CGTCTCTACT TAAGACGGTG AAGGCGGTGG AGGATGAAGC CACTAAAGGC 
ACACGAGCAC TAGAGGCCAC CATAGAGCAC ATCAAACAAG AGCTGGCTGT TTTCAGCAGT 
GCAGATCCTC CTCCAAAGAC GGCCACTCCA GAGGAGTTCA TCCGCATGAC CAAAGGCATC 
ACATTGGCCA CAGCTAAAGC AGTGGCTGCT GGGAACTCCT GCCGTCAGGA GGACGTGATC 
GCCACTGCTA ACCTAAGCAG ACGAGCTGTC GCAGACATGC TGCGCTCCTG CAAGCAAGCC 
GCATACCACC CGGAGGTGAA GCGGGAGGTC CAGACTCGAG CGCTCCGCTT TGGCTCTGAG 
TGCTCCACAA GTTACCTGGG CCTGCTGGAG CACGTGTTAG TGATCATTCA GAAACCCTCG 
CATGACCTTA AACAGCAGCT GGCAAACTAC TCCAAACGTG TGGCCAGTTC AGTGACCGAG 
CTCATCCAGG CTGCGGAGGC CATGAAAGGT ACGGAGTGGG TGGATCCCGA AGACCCCACC 
GTCATTGCAG AAAACGAGCT GCTGGGAGCC GCAGCTGCCA TTGAAGCTGC CGCGAAGAAA 
CTAGAGCAGC TCAAACCCCG AGCCAAACCC AAGGAAGCTG ATGAGAGTCT GAACTTTGAG 
GAACAGATTC TAGAAGCGGC CAAATCCATC GCTGCAGCCA CCAGTGCTCT GGTCAAAGCT 
GCGTCTGCCG CTCAGAGAGA GCTGGTGGCT CAAGGGAAGG TTGGGGCAAT TCCAGCAAAC 
GCGGTGGATG ATGGACAGTG GTCTCAGGGT CTCATTTCAG CTGCTCGTAT GGTAGCCGCA 
GCTACTAACA ACCTGTGCGA AGCAGCTAAC TCTGCGGTTC AGGGTCACGC CAGCGAGGAG 
AAACTCATTT CTTCAGCCAA ACAGGTGGCA GCATCCACCG CACAGCTGCT GGTGGCCTGT 
AAGGTCAAGG CTGACCAGGA CTCCCAGACC ATGAAGAGAC TGCAGGCTGC TGGTAATGCT 
GTCAAGAGGG CGTCTGATAA CCTGGTGAAG GCGGCACAGA AGGCTGCGTT TGAGGCTCAA 
GATGATCAGG CTGTGATGGT GAAGAGCAAG ATGGTGGGCG GTATTGCTCA GATCATCGCT 
GCTCAGGAGG AGATGCTCAG GAAGGAGAAA GAGCTGGAAG AAGCCAGAAA GAAACTGGCA 
ATGATCCGAC AGCAGCAGTA CAAGTTCCTG CCCTCAGAGC TGCGAGAGGA CGGACAGGAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155557.1
CDS	202..7827
Translation	MVALSLKIGVGNVVKTMQFEPSTVVYDACRIIRERVPEAQLGQPSDYGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTLEYKKKQRPLKIRMLDGTVKTVMVDDSKIVSDMLMTICARIGITNYDEYSLVRDVGEEKKEETTGTLKRDKTLLRDDKKMEKLKQKLHTDDELNWLDHGRTLREQGVEESEMLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGYQCQIQFGDHNESKHKPGFLDLKEFLPKEYVKNKGEKRIFQAHKNCQNMTEIEAKVNYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKESVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQFNKMGKVETGSVALPAIIRSGAGGPESMKMGSMPQAKQHITSGQMHRGHMPPLTSAQQALTGTINSSMQAVQAVQASLDEFDALPPLGTDSASQAWRKNKMDESKHEIHSQVDAITAGTASMVNLTAGDPAETDYTAVGCAVTTISSNLTEMSKGVKLLAALMEDEGGNGQQLLGAAKNLACAVSDMLKTAQPSSTEPRQNLLQAAGNVGQASGELLQQIGESDADQHFQDMLMQLAKAVANAAAALVLKAKNVAQKSEDSAQQNKVIAAATQCALSTSQLVACTRVVAPTISSPVCQEQLIEAGKLVAKSVEGCVEASHGATGDEHLLKQVGAAATGVTQALNELLQHIRQYASGGQPIGRHGEATDRILDVTDNIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGESDLENSRKLLSAAKLLADATARMVEAAKGAAANPDSEEQQQKLREAAEGLRMATNAAAQNAIKKRLVNKLENAAKQAAAAATQTIAAAQHAASSNKNPAAQQQLVQSCKVVADQIPQLVQGLRSSQAQPDSPSAQLALIGASQNFLQPGAKMVTAAKATVPTISDQASAMQLSQCAKNLASALAELRTAAQKAQEACGPLEIDNALTMVRGLERDMQEAKASAAEGKLRPLPGETLEKCSQDLGSSTKAVSSAIAQLLSEATQGNENYTGMAARDVAQALRSLASAARGVAANTEDPLARNAMLDCTGDVMDKSASLIEETKRAIAKPGDPDSQQRLAQVAKAVSQALNRSVNCLPGQRDVDNAIRTVGEASKKLLSDKFPASGKNFQEAQANLNEAAAGLNQSANELVQASRGTTQDLAKASGKFGQDFNQFLQAGVDMAGQSQSKEDQTQVVTNLKTISMSSSKLLLAAKALSTDPNSPNLKNQLAAAARAVTDSINQLISMCTQQAPGQKECDNALRELETVGGMLENPTEAVNDLGYFDCIDSVMENSKVLGEAMAGISHNAKNSNLPEFGDSVSSASKALCGLTEAAAQGAYLVGVSDPNSHAGQKGLVDPSQFARAKQSIQMACQNLVDPACTQSQVLSAATIVAKHTSALCNACRLASSKTSNPVAKRQFVQSAKEVANSTANLVKSIKALDGAFNQENRQKCKEATGPLIEAVDNLTAFASNPEFASVPAQISPEGLAAMEPITAAAKAMLESSTGLIQTARSLAVNPKDPPKWSVLAGHSRTVSDSIKKLITNMREKAPGQRECDDAIEVLSNCIREVDKASLAAISQQLTPRDDISHEALHEQMAASVHEISNLIEPVAIAARADASQLGHKVSQMVSYFEPLIMAAIGTASKILNSQQQMNVLDQTKTLAESALQMLYTAKETGGNHKAAHTQEALEESVQMMKEAVDDLGSTLAEVASAAGAVGGMVNSITQSINKMEDGPAFEPEGTFVDYQTTMVKTAKAIAVTVQEMVTKSNTNPDELGGLANQLTTEFGDLASEAKCAAMTAENDEIGSHIKKQVTELGYTCTGLVSKAGALQCSPNDSYTKKELIDSARKVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNRENAETFADHREHILKTAKVLVEDTKLLVSGAGASQEKLAQAAQSSVNTITKLADVVKLGAASLGSEDPETQVVLINAVKDVAKALGDLISATKAAAGKPHDDPAMLQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATIEHIKQELAVFSSADPPPKTATPEEFIRMTKGITLATAKAVAAGNSCRQEDVIATANLSRRAVADMLRSCKQAAYHPEVKREVQTRALRFGSECSTSYLGLLEHVLVIIQKPSHDLKQQLANYSKRVASSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATNNLCEAANSAVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSQTMKRLQAAGNAVKRASDNLVKAAQKAAFEAQDDQAVMVKSKMVGGIAQIIAAQEEMLRKEKELEEARKKLAMIRQQQYKFLPSELREDGQEQ

Target ORF information:

RefSeq Version	XM_005155500.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio talin 1 (tln1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155500.4

ORF Insert Sequence:

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGGACAT GTTGATGCAG TTGGCGAAGG CTGTGGCGAA TGCAGCAGCG 
GCTCTGGTGT TAAAGGCTAA AAACGTGGCT CAGAAAAGCG AAGATTCAGC TCAACAAAAC 
AAAGTGATCG CTGCTGCCAC CCAGTGCGCC CTCTCCACAT CACAGCTGGT GGCCTGCACA 
CGAGTGGTGG CTCCAACCAT CAGCTCACCC GTGTGTCAAG AGCAGCTCAT CGAGGCTGGA 
AAACTGGTGG CGAAGTCTGT GGAGGGCTGT GTGGAGGCGT CACACGGGGC GACCGGTGAC 
GAGCATCTGC TCAAACAGGT GGGCGCCGCT GCCACAGGAG TCACTCAGGC TCTTAACGAG 
CTGCTGCAGC ATATTAGACA GTACGCCAGC GGAGGACAGC CAATCGGACG GCACGGAGAG 
GCTACCGACC GCATCCTGGA TGTGACTGAT AACATCTTCA GCTCCATGGG AGATGCAGGG 
GAGATGGTGC GTCAGGCGCG GATCCTTGCT CAGGCCACCT CAGACCTGGT CAACGCGATC 
AAGGCGGACG CAGAGGGAGA ATCTGACCTG GAGAACTCTC GCAAACTCCT CTCTGCAGCC 
AAACTCCTGG CTGACGCTAC TGCCCGTATG GTGGAAGCTG CGAAGGGTGC AGCTGCTAAT 
CCAGACAGCG AGGAGCAGCA GCAGAAGTTG CGTGAAGCTG CAGAGGGACT GCGCATGGCC 
ACCAATGCTG CAGCACAGAA CGCCATTAAA AAGAGACTCG TCAACAAACT GGAGAATGCA 
GCGAAACAAG CCGCAGCAGC CGCCACTCAG ACCATAGCAG CCGCTCAACA TGCAGCTTCA 
TCCAACAAGA ACCCTGCGGC CCAGCAACAA CTCGTACAGA GCTGCAAGGT GGTGGCGGAT 
CAGATCCCAC AGCTGGTTCA GGGTCTGAGG AGCAGCCAGG CTCAACCTGA CAGCCCCAGC 
GCTCAACTCG CCCTCATCGG AGCTAGCCAG AACTTCCTGC AGCCCGGAGC CAAAATGGTG 
ACCGCAGCCA AAGCTACAGT TCCAACCATC AGTGACCAGG CGTCTGCCAT GCAGCTCAGT 
CAGTGCGCTA AAAACCTGGC CAGTGCTCTG GCTGAGCTCC GCACGGCCGC CCAGAAGGCT 
CAGGAGGCGT GTGGTCCGTT GGAGATTGAT AACGCTCTGA CTATGGTACG GGGGCTGGAG 
AGGGACATGC AGGAGGCCAA AGCTTCAGCT GCTGAGGGCA AACTCAGACC CCTGCCTGGA 
GAAACGCTGG AGAAGTGCTC TCAGGATCTG GGCTCCAGCA CTAAAGCCGT GAGCTCTGCC 
ATTGCTCAGC TCCTCAGTGA AGCAACTCAA GGCAATGAGA ACTACACAGG TATGGCTGCT 
CGTGATGTGG CTCAGGCTCT GCGCTCGCTG GCTTCAGCCG CTCGTGGAGT GGCAGCCAAC 
ACTGAAGATC CTCTAGCCCG TAACGCAATG CTGGACTGCA CCGGCGATGT CATGGACAAA 
TCCGCCAGCC TGATTGAAGA GACCAAGAGG GCCATAGCCA AACCAGGAGA CCCTGACAGT 
CAGCAAAGAC TGGCCCAGGT AGCCAAGGCT GTGTCTCAGG CACTGAACCG CTCTGTAAAC 
TGTCTGCCGG GGCAGAGGGA TGTGGATAAT GCCATCAGAA CTGTCGGGGA AGCTAGCAAG 
AAACTGCTGT CTGATAAATT TCCAGCGAGT GGGAAGAATT TCCAAGAGGC TCAGGCCAAT 
CTGAACGAGG CTGCAGCTGG ACTAAACCAG TCAGCCAATG AGCTGGTGCA GGCCTCGCGT 
GGCACCACCC AGGACCTGGC CAAAGCCTCC GGGAAGTTTG GACAAGACTT TAACCAATTC 
CTGCAGGCTG GAGTGGACAT GGCTGGACAA TCCCAGTCTA AAGAAGACCA AACTCAAGTG 
GTCACCAACC TGAAGACCAT CTCCATGTCC TCCAGCAAAC TCCTGCTGGC GGCTAAAGCT 
CTGTCCACAG ACCCCAACTC GCCAAATCTG AAGAACCAAC TCGCTGCTGC TGCTCGCGCG 
GTGACAGACA GCATTAATCA GCTGATCAGC ATGTGTACCC AGCAGGCTCC GGGACAGAAG 
GAGTGTGACA ACGCTCTCCG TGAGCTTGAG ACGGTTGGAG GAATGCTGGA GAACCCAACA 
GAAGCAGTCA ATGATTTGGG CTACTTTGAC TGCATTGACA GTGTGATGGA GAACTCAAAG 
GTTTTGGGTG AGGCCATGGC TGGCATTTCC CATAATGCCA AAAACAGTAA CTTGCCAGAG 
TTTGGAGATT CGGTCAGTTC AGCGTCTAAA GCGCTGTGTG GTCTCACTGA AGCAGCTGCT 
CAGGGGGCGT ATCTGGTTGG TGTGTCAGAC CCGAACAGCC ATGCTGGCCA GAAGGGTCTT 
GTGGACCCCT CACAATTTGC CAGAGCCAAA CAGTCCATCC AGATGGCCTG TCAGAACCTG 
GTGGATCCAG CCTGCACCCA GTCCCAGGTT CTCTCTGCTG CCACCATCGT TGCCAAACAC 
ACTTCGGCCT TGTGCAATGC CTGCCGGCTC GCCTCCTCCA AGACATCCAA CCCAGTTGCT 
AAGAGACAGT TCGTCCAGAG TGCCAAAGAG GTGGCCAACA GCACAGCCAA CCTCGTCAAG 
TCCATCAAGG CTCTCGACGG GGCCTTTAAC CAGGAAAACA GACAGAAGTG TAAGGAAGCC 
ACTGGGCCAC TCATTGAGGC TGTGGATAAC CTGACTGCCT TCGCTTCAAA CCCAGAGTTC 
GCAAGTGTAC CCGCTCAGAT CAGCCCAGAG GGTCTGGCTG CGATGGAGCC GATCACAGCA 
GCCGCTAAGG CAATGTTGGA AAGCTCGACA GGACTGATCC AGACGGCTCG CTCGCTCGCA 
GTCAACCCGA AAGATCCACC CAAGTGGTCT GTGCTTGCTG GACACTCCAG AACAGTGTCC 
GACTCCATCA AGAAACTCAT CACTAACATG AGGGAAAAGG CTCCGGGTCA GAGGGAGTGT 
GATGATGCCA TTGAGGTTCT TAGTAACTGC ATTCGTGAAG TGGACAAGGC CTCGCTGGCA 
GCCATCAGTC AGCAGCTCAC GCCTAGAGAC GACATTTCCC ATGAGGCTCT GCACGAGCAG 
ATGGCGGCGT CTGTGCATGA GATCAGTAAC CTGATTGAAC CAGTGGCCAT CGCAGCCCGT 
GCTGATGCCT CTCAGCTGGG ACACAAGGTG TCTCAGATGG TCAGTTACTT TGAACCGCTC 
ATCATGGCTG CCATCGGCAC AGCGTCTAAG ATCCTGAACA GCCAGCAGCA GATGAACGTG 
CTGGACCAAA CCAAGACTCT GGCTGAGTCT GCGCTACAGA TGCTCTATAC GGCCAAAGAG 
ACTGGAGGAA ACCACAAGGC GGCGCACACA CAAGAGGCTC TGGAGGAGTC TGTTCAGATG 
ATGAAGGAGG CGGTGGACGA TCTGGGTTCG ACTCTGGCAG AAGTGGCCAG CGCTGCAGGA 
GCAGTGGGAG GAATGGTCAA CTCCATCACT CAGTCCATCA ACAAGATGGA GGACGGACCT 
GCCTTCGAGC CTGAAGGAAC GTTTGTGGAT TATCAGACCA CCATGGTGAA GACAGCCAAA 
GCTATCGCTG TCACCGTTCA GGAGATGGTT ACCAAATCCA ACACCAACCC TGATGAGCTG 
GGCGGTTTGG CCAATCAGCT GACCACAGAG TTCGGTGACC TCGCATCAGA GGCCAAATGT 
GCAGCCATGA CTGCTGAAAA CGATGAGATC GGTTCACATA TCAAGAAGCA GGTGACTGAG 
TTGGGCTACA CCTGCACTGG TCTGGTCAGT AAAGCAGGAG CGCTACAGTG CAGTCCAAAC 
GACTCCTACA CTAAGAAGGA GCTCATTGAC AGTGCCAGGA AAGTCTCTGA GAAGGTCTCT 
CATGTGCTGG CAGCTCTTCA GGCAGGCAAT CGTGGCACTC AGGCCTGCAT TACTGCCGCG 
AGCGCTGTCT CTGGCATCAT TGCTGATTTA GACACCACCA TCATGTTTGC CACCGCGGGC 
ACTCTCAACC GAGAGAACGC AGAGACATTT GCAGACCACA GAGAGCACAT CTTAAAGACG 
GCTAAAGTTC TGGTGGAGGA CACGAAGCTG CTGGTTTCTG GAGCCGGAGC AAGTCAGGAG 
AAACTGGCTC AGGCTGCACA GTCCTCCGTC AACACCATCA CCAAACTGGC CGATGTGGTT 
AAATTGGGGG CAGCGAGTCT GGGCTCTGAA GACCCAGAGA CACAGGTGGT TCTGATCAAC 
GCTGTGAAGG ACGTGGCTAA AGCTCTGGGT GACCTGATCA GCGCCACCAA AGCAGCGGCC 
GGCAAACCTC ACGATGACCC CGCCATGCTG CAGCTCAAAA ACTCTGCTAA GGTGATGGTG 
ACCAATGTGA CGTCTCTACT TAAGACGGTG AAGGCGGTGG AGGATGAAGC CACTAAAGGC 
ACACGAGCAC TAGAGGCCAC CATAGAGCAC ATCAAACAAG AGCTGGCTGT TTTCAGCAGT 
GCAGATCCTC CTCCAAAGAC GGCCACTCCA GAGGAGTTCA TCCGCATGAC CAAAGGCATC 
ACATTGGCCA CAGCTAAAGC AGTGGCTGCT GGGAACTCCT GCCGTCAGGA GGACGTGATC 
GCCACTGCTA ACCTAAGCAG ACGAGCTGTC GCAGACATGC TGCGCTCCTG CAAGCAAGCC 
GCATACCACC CGGAGGTGAA GCGGGAGGTC CAGACTCGAG CGCTCCGCTT TGGCTCTGAG 
TGCTCCACAA GTTACCTGGG CCTGCTGGAG CACGTGTTAG TGATCATTCA GAAACCCTCG 
CATGACCTTA AACAGCAGCT GGCAAACTAC TCCAAACGTG TGGCCAGTTC AGTGACCGAG 
CTCATCCAGG CTGCGGAGGC CATGAAAGGT ACGGAGTGGG TGGATCCCGA AGACCCCACC 
GTCATTGCAG AAAACGAGCT GCTGGGAGCC GCAGCTGCCA TTGAAGCTGC CGCGAAGAAA 
CTAGAGCAGC TCAAACCCCG AGCCAAACCC AAGGAAGCTG ATGAGAGTCT GAACTTTGAG 
GAACAGATTC TAGAAGCGGC CAAATCCATC GCTGCAGCCA CCAGTGCTCT GGTCAAAGCT 
GCGTCTGCCG CTCAGAGAGA GCTGGTGGCT CAAGGGAAGG TTGGGGCAAT TCCAGCAAAC 
GCGGTGGATG ATGGACAGTG GTCTCAGGGT CTCATTTCAG CTGCTCGTAT GGTAGCCGCA 
GCTACTAACA ACCTGTGCGA AGCAGCTAAC TCTGCGGTTC AGGGTCACGC CAGCGAGGAG 
AAACTCATTT CTTCAGCCAA ACAGGTGGCA GCATCCACCG CACAGCTGCT GGTGGCCTGT 
AAGGTCAAGG CTGACCAGGA CTCCCAGACC ATGAAGAGAC TGCAGGCTGC TGGTAATGCT 
GTCAAGAGGG CGTCTGATAA CCTGGTGAAG GCGGCACAGA AGGCTGCGTT TGAGGCTCAA 
GATGATCAGG CTGTGATGGT GAAGAGCAAG ATGGTGGGCG GTATTGCTCA GATCATCGCT 
GCTCAGGAGG AGATGCTCAG GAAGGAGAAA GAGCTGGAAG AAGCCAGAAA GAAACTGGCA 
ATGATCCGAC AGCAGCAGTA CAAGTTCCTG CCCTCAGAGC TGCGAGAGGA CGGACAGGAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tln1 ( XM_005155500.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155557.1 Danio rerio talin 1 (tln1), transcript variant X2, mRNA.

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CloneID	ODa15806
Clone ID Related Accession (Same CDS sequence)	XM_005155500.4 , XM_005155500.3
Accession Version	XM_005155500.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	talin-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155500.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGGACAT GTTGATGCAG TTGGCGAAGG CTGTGGCGAA TGCAGCAGCG 
GCTCTGGTGT TAAAGGCTAA AAACGTGGCT CAGAAAAGCG AAGATTCAGC TCAACAAAAC 
AAAGTGATCG CTGCTGCCAC CCAGTGCGCC CTCTCCACAT CACAGCTGGT GGCCTGCACA 
CGAGTGGTGG CTCCAACCAT CAGCTCACCC GTGTGTCAAG AGCAGCTCAT CGAGGCTGGA 
AAACTGGTGG CGAAGTCTGT GGAGGGCTGT GTGGAGGCGT CACACGGGGC GACCGGTGAC 
GAGCATCTGC TCAAACAGGT GGGCGCCGCT GCCACAGGAG TCACTCAGGC TCTTAACGAG 
CTGCTGCAGC ATATTAGACA GTACGCCAGC GGAGGACAGC CAATCGGACG GCACGGAGAG 
GCTACCGACC GCATCCTGGA TGTGACTGAT AACATCTTCA GCTCCATGGG AGATGCAGGG 
GAGATGGTGC GTCAGGCGCG GATCCTTGCT CAGGCCACCT CAGACCTGGT CAACGCGATC 
AAGGCGGACG CAGAGGGAGA ATCTGACCTG GAGAACTCTC GCAAACTCCT CTCTGCAGCC 
AAACTCCTGG CTGACGCTAC TGCCCGTATG GTGGAAGCTG CGAAGGGTGC AGCTGCTAAT 
CCAGACAGCG AGGAGCAGCA GCAGAAGTTG CGTGAAGCTG CAGAGGGACT GCGCATGGCC 
ACCAATGCTG CAGCACAGAA CGCCATTAAA AAGAGACTCG TCAACAAACT GGAGAATGCA 
GCGAAACAAG CCGCAGCAGC CGCCACTCAG ACCATAGCAG CCGCTCAACA TGCAGCTTCA 
TCCAACAAGA ACCCTGCGGC CCAGCAACAA CTCGTACAGA GCTGCAAGGT GGTGGCGGAT 
CAGATCCCAC AGCTGGTTCA GGGTCTGAGG AGCAGCCAGG CTCAACCTGA CAGCCCCAGC 
GCTCAACTCG CCCTCATCGG AGCTAGCCAG AACTTCCTGC AGCCCGGAGC CAAAATGGTG 
ACCGCAGCCA AAGCTACAGT TCCAACCATC AGTGACCAGG CGTCTGCCAT GCAGCTCAGT 
CAGTGCGCTA AAAACCTGGC CAGTGCTCTG GCTGAGCTCC GCACGGCCGC CCAGAAGGCT 
CAGGAGGCGT GTGGTCCGTT GGAGATTGAT AACGCTCTGA CTATGGTACG GGGGCTGGAG 
AGGGACATGC AGGAGGCCAA AGCTTCAGCT GCTGAGGGCA AACTCAGACC CCTGCCTGGA 
GAAACGCTGG AGAAGTGCTC TCAGGATCTG GGCTCCAGCA CTAAAGCCGT GAGCTCTGCC 
ATTGCTCAGC TCCTCAGTGA AGCAACTCAA GGCAATGAGA ACTACACAGG TATGGCTGCT 
CGTGATGTGG CTCAGGCTCT GCGCTCGCTG GCTTCAGCCG CTCGTGGAGT GGCAGCCAAC 
ACTGAAGATC CTCTAGCCCG TAACGCAATG CTGGACTGCA CCGGCGATGT CATGGACAAA 
TCCGCCAGCC TGATTGAAGA GACCAAGAGG GCCATAGCCA AACCAGGAGA CCCTGACAGT 
CAGCAAAGAC TGGCCCAGGT AGCCAAGGCT GTGTCTCAGG CACTGAACCG CTCTGTAAAC 
TGTCTGCCGG GGCAGAGGGA TGTGGATAAT GCCATCAGAA CTGTCGGGGA AGCTAGCAAG 
AAACTGCTGT CTGATAAATT TCCAGCGAGT GGGAAGAATT TCCAAGAGGC TCAGGCCAAT 
CTGAACGAGG CTGCAGCTGG ACTAAACCAG TCAGCCAATG AGCTGGTGCA GGCCTCGCGT 
GGCACCACCC AGGACCTGGC CAAAGCCTCC GGGAAGTTTG GACAAGACTT TAACCAATTC 
CTGCAGGCTG GAGTGGACAT GGCTGGACAA TCCCAGTCTA AAGAAGACCA AACTCAAGTG 
GTCACCAACC TGAAGACCAT CTCCATGTCC TCCAGCAAAC TCCTGCTGGC GGCTAAAGCT 
CTGTCCACAG ACCCCAACTC GCCAAATCTG AAGAACCAAC TCGCTGCTGC TGCTCGCGCG 
GTGACAGACA GCATTAATCA GCTGATCAGC ATGTGTACCC AGCAGGCTCC GGGACAGAAG 
GAGTGTGACA ACGCTCTCCG TGAGCTTGAG ACGGTTGGAG GAATGCTGGA GAACCCAACA 
GAAGCAGTCA ATGATTTGGG CTACTTTGAC TGCATTGACA GTGTGATGGA GAACTCAAAG 
GTTTTGGGTG AGGCCATGGC TGGCATTTCC CATAATGCCA AAAACAGTAA CTTGCCAGAG 
TTTGGAGATT CGGTCAGTTC AGCGTCTAAA GCGCTGTGTG GTCTCACTGA AGCAGCTGCT 
CAGGGGGCGT ATCTGGTTGG TGTGTCAGAC CCGAACAGCC ATGCTGGCCA GAAGGGTCTT 
GTGGACCCCT CACAATTTGC CAGAGCCAAA CAGTCCATCC AGATGGCCTG TCAGAACCTG 
GTGGATCCAG CCTGCACCCA GTCCCAGGTT CTCTCTGCTG CCACCATCGT TGCCAAACAC 
ACTTCGGCCT TGTGCAATGC CTGCCGGCTC GCCTCCTCCA AGACATCCAA CCCAGTTGCT 
AAGAGACAGT TCGTCCAGAG TGCCAAAGAG GTGGCCAACA GCACAGCCAA CCTCGTCAAG 
TCCATCAAGG CTCTCGACGG GGCCTTTAAC CAGGAAAACA GACAGAAGTG TAAGGAAGCC 
ACTGGGCCAC TCATTGAGGC TGTGGATAAC CTGACTGCCT TCGCTTCAAA CCCAGAGTTC 
GCAAGTGTAC CCGCTCAGAT CAGCCCAGAG GGTCTGGCTG CGATGGAGCC GATCACAGCA 
GCCGCTAAGG CAATGTTGGA AAGCTCGACA GGACTGATCC AGACGGCTCG CTCGCTCGCA 
GTCAACCCGA AAGATCCACC CAAGTGGTCT GTGCTTGCTG GACACTCCAG AACAGTGTCC 
GACTCCATCA AGAAACTCAT CACTAACATG AGGGAAAAGG CTCCGGGTCA GAGGGAGTGT 
GATGATGCCA TTGAGGTTCT TAGTAACTGC ATTCGTGAAG TGGACAAGGC CTCGCTGGCA 
GCCATCAGTC AGCAGCTCAC GCCTAGAGAC GACATTTCCC ATGAGGCTCT GCACGAGCAG 
ATGGCGGCGT CTGTGCATGA GATCAGTAAC CTGATTGAAC CAGTGGCCAT CGCAGCCCGT 
GCTGATGCCT CTCAGCTGGG ACACAAGGTG TCTCAGATGG TCAGTTACTT TGAACCGCTC 
ATCATGGCTG CCATCGGCAC AGCGTCTAAG ATCCTGAACA GCCAGCAGCA GATGAACGTG 
CTGGACCAAA CCAAGACTCT GGCTGAGTCT GCGCTACAGA TGCTCTATAC GGCCAAAGAG 
ACTGGAGGAA ACCACAAGGC GGCGCACACA CAAGAGGCTC TGGAGGAGTC TGTTCAGATG 
ATGAAGGAGG CGGTGGACGA TCTGGGTTCG ACTCTGGCAG AAGTGGCCAG CGCTGCAGGA 
GCAGTGGGAG GAATGGTCAA CTCCATCACT CAGTCCATCA ACAAGATGGA GGACGGACCT 
GCCTTCGAGC CTGAAGGAAC GTTTGTGGAT TATCAGACCA CCATGGTGAA GACAGCCAAA 
GCTATCGCTG TCACCGTTCA GGAGATGGTT ACCAAATCCA ACACCAACCC TGATGAGCTG 
GGCGGTTTGG CCAATCAGCT GACCACAGAG TTCGGTGACC TCGCATCAGA GGCCAAATGT 
GCAGCCATGA CTGCTGAAAA CGATGAGATC GGTTCACATA TCAAGAAGCA GGTGACTGAG 
TTGGGCTACA CCTGCACTGG TCTGGTCAGT AAAGCAGGAG CGCTACAGTG CAGTCCAAAC 
GACTCCTACA CTAAGAAGGA GCTCATTGAC AGTGCCAGGA AAGTCTCTGA GAAGGTCTCT 
CATGTGCTGG CAGCTCTTCA GGCAGGCAAT CGTGGCACTC AGGCCTGCAT TACTGCCGCG 
AGCGCTGTCT CTGGCATCAT TGCTGATTTA GACACCACCA TCATGTTTGC CACCGCGGGC 
ACTCTCAACC GAGAGAACGC AGAGACATTT GCAGACCACA GAGAGCACAT CTTAAAGACG 
GCTAAAGTTC TGGTGGAGGA CACGAAGCTG CTGGTTTCTG GAGCCGGAGC AAGTCAGGAG 
AAACTGGCTC AGGCTGCACA GTCCTCCGTC AACACCATCA CCAAACTGGC CGATGTGGTT 
AAATTGGGGG CAGCGAGTCT GGGCTCTGAA GACCCAGAGA CACAGGTGGT TCTGATCAAC 
GCTGTGAAGG ACGTGGCTAA AGCTCTGGGT GACCTGATCA GCGCCACCAA AGCAGCGGCC 
GGCAAACCTC ACGATGACCC CGCCATGCTG CAGCTCAAAA ACTCTGCTAA GGTGATGGTG 
ACCAATGTGA CGTCTCTACT TAAGACGGTG AAGGCGGTGG AGGATGAAGC CACTAAAGGC 
ACACGAGCAC TAGAGGCCAC CATAGAGCAC ATCAAACAAG AGCTGGCTGT TTTCAGCAGT 
GCAGATCCTC CTCCAAAGAC GGCCACTCCA GAGGAGTTCA TCCGCATGAC CAAAGGCATC 
ACATTGGCCA CAGCTAAAGC AGTGGCTGCT GGGAACTCCT GCCGTCAGGA GGACGTGATC 
GCCACTGCTA ACCTAAGCAG ACGAGCTGTC GCAGACATGC TGCGCTCCTG CAAGCAAGCC 
GCATACCACC CGGAGGTGAA GCGGGAGGTC CAGACTCGAG CGCTCCGCTT TGGCTCTGAG 
TGCTCCACAA GTTACCTGGG CCTGCTGGAG CACGTGTTAG TGATCATTCA GAAACCCTCG 
CATGACCTTA AACAGCAGCT GGCAAACTAC TCCAAACGTG TGGCCAGTTC AGTGACCGAG 
CTCATCCAGG CTGCGGAGGC CATGAAAGGT ACGGAGTGGG TGGATCCCGA AGACCCCACC 
GTCATTGCAG AAAACGAGCT GCTGGGAGCC GCAGCTGCCA TTGAAGCTGC CGCGAAGAAA 
CTAGAGCAGC TCAAACCCCG AGCCAAACCC AAGGAAGCTG ATGAGAGTCT GAACTTTGAG 
GAACAGATTC TAGAAGCGGC CAAATCCATC GCTGCAGCCA CCAGTGCTCT GGTCAAAGCT 
GCGTCTGCCG CTCAGAGAGA GCTGGTGGCT CAAGGGAAGG TTGGGGCAAT TCCAGCAAAC 
GCGGTGGATG ATGGACAGTG GTCTCAGGGT CTCATTTCAG CTGCTCGTAT GGTAGCCGCA 
GCTACTAACA ACCTGTGCGA AGCAGCTAAC TCTGCGGTTC AGGGTCACGC CAGCGAGGAG 
AAACTCATTT CTTCAGCCAA ACAGGTGGCA GCATCCACCG CACAGCTGCT GGTGGCCTGT 
AAGGTCAAGG CTGACCAGGA CTCCCAGACC ATGAAGAGAC TGCAGGCTGC TGGTAATGCT 
GTCAAGAGGG CGTCTGATAA CCTGGTGAAG GCGGCACAGA AGGCTGCGTT TGAGGCTCAA 
GATGATCAGG CTGTGATGGT GAAGAGCAAG ATGGTGGGCG GTATTGCTCA GATCATCGCT 
GCTCAGGAGG AGATGCTCAG GAAGGAGAAA GAGCTGGAAG AAGCCAGAAA GAAACTGGCA 
ATGATCCGAC AGCAGCAGTA CAAGTTCCTG CCCTCAGAGC TGCGAGAGGA CGGACAGGAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155557.1
CDS	196..7821
Translation	MVALSLKIGVGNVVKTMQFEPSTVVYDACRIIRERVPEAQLGQPSDYGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTLEYKKKQRPLKIRMLDGTVKTVMVDDSKIVSDMLMTICARIGITNYDEYSLVRDVGEEKKEETTGTLKRDKTLLRDDKKMEKLKQKLHTDDELNWLDHGRTLREQGVEESEMLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGYQCQIQFGDHNESKHKPGFLDLKEFLPKEYVKNKGEKRIFQAHKNCQNMTEIEAKVNYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKESVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQFNKMGKVETGSVALPAIIRSGAGGPESMKMGSMPQAKQHITSGQMHRGHMPPLTSAQQALTGTINSSMQAVQAVQASLDEFDALPPLGTDSASQAWRKNKMDESKHEIHSQVDAITAGTASMVNLTAGDPAETDYTAVGCAVTTISSNLTEMSKGVKLLAALMEDEGGNGQQLLGAAKNLACAVSDMLKTAQPSSTEPRQNLLQAAGNVGQASGELLQQIGESDADQHFQDMLMQLAKAVANAAAALVLKAKNVAQKSEDSAQQNKVIAAATQCALSTSQLVACTRVVAPTISSPVCQEQLIEAGKLVAKSVEGCVEASHGATGDEHLLKQVGAAATGVTQALNELLQHIRQYASGGQPIGRHGEATDRILDVTDNIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGESDLENSRKLLSAAKLLADATARMVEAAKGAAANPDSEEQQQKLREAAEGLRMATNAAAQNAIKKRLVNKLENAAKQAAAAATQTIAAAQHAASSNKNPAAQQQLVQSCKVVADQIPQLVQGLRSSQAQPDSPSAQLALIGASQNFLQPGAKMVTAAKATVPTISDQASAMQLSQCAKNLASALAELRTAAQKAQEACGPLEIDNALTMVRGLERDMQEAKASAAEGKLRPLPGETLEKCSQDLGSSTKAVSSAIAQLLSEATQGNENYTGMAARDVAQALRSLASAARGVAANTEDPLARNAMLDCTGDVMDKSASLIEETKRAIAKPGDPDSQQRLAQVAKAVSQALNRSVNCLPGQRDVDNAIRTVGEASKKLLSDKFPASGKNFQEAQANLNEAAAGLNQSANELVQASRGTTQDLAKASGKFGQDFNQFLQAGVDMAGQSQSKEDQTQVVTNLKTISMSSSKLLLAAKALSTDPNSPNLKNQLAAAARAVTDSINQLISMCTQQAPGQKECDNALRELETVGGMLENPTEAVNDLGYFDCIDSVMENSKVLGEAMAGISHNAKNSNLPEFGDSVSSASKALCGLTEAAAQGAYLVGVSDPNSHAGQKGLVDPSQFARAKQSIQMACQNLVDPACTQSQVLSAATIVAKHTSALCNACRLASSKTSNPVAKRQFVQSAKEVANSTANLVKSIKALDGAFNQENRQKCKEATGPLIEAVDNLTAFASNPEFASVPAQISPEGLAAMEPITAAAKAMLESSTGLIQTARSLAVNPKDPPKWSVLAGHSRTVSDSIKKLITNMREKAPGQRECDDAIEVLSNCIREVDKASLAAISQQLTPRDDISHEALHEQMAASVHEISNLIEPVAIAARADASQLGHKVSQMVSYFEPLIMAAIGTASKILNSQQQMNVLDQTKTLAESALQMLYTAKETGGNHKAAHTQEALEESVQMMKEAVDDLGSTLAEVASAAGAVGGMVNSITQSINKMEDGPAFEPEGTFVDYQTTMVKTAKAIAVTVQEMVTKSNTNPDELGGLANQLTTEFGDLASEAKCAAMTAENDEIGSHIKKQVTELGYTCTGLVSKAGALQCSPNDSYTKKELIDSARKVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNRENAETFADHREHILKTAKVLVEDTKLLVSGAGASQEKLAQAAQSSVNTITKLADVVKLGAASLGSEDPETQVVLINAVKDVAKALGDLISATKAAAGKPHDDPAMLQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATIEHIKQELAVFSSADPPPKTATPEEFIRMTKGITLATAKAVAAGNSCRQEDVIATANLSRRAVADMLRSCKQAAYHPEVKREVQTRALRFGSECSTSYLGLLEHVLVIIQKPSHDLKQQLANYSKRVASSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATNNLCEAANSAVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSQTMKRLQAAGNAVKRASDNLVKAAQKAAFEAQDDQAVMVKSKMVGGIAQIIAAQEEMLRKEKELEEARKKLAMIRQQQYKFLPSELREDGQEQ

Target ORF information:

RefSeq Version	XM_005155500.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio talin 1 (tln1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155500.3

ORF Insert Sequence:

ATGGTGGCGC TGTCGCTGAA GATCGGGGTG GGGAATGTGG TGAAGACGAT GCAGTTTGAG 
CCCTCCACAG TGGTGTACGA CGCCTGTCGA ATCATCAGGG AGAGAGTTCC AGAAGCACAA 
CTCGGCCAGC CCAGTGACTA TGGCCTGTTC CTGTCAGACG ATGATCCAAA GAAGGGCATT 
TGGCTGGAAG CAGGCAAAGC CCTGGATTAC TACATGCTTC GAAACGGGGA TACTCTGGAG 
TACAAAAAGA AACAGAGACC CCTGAAGATT CGCATGTTGG ATGGAACGGT CAAAACAGTT 
ATGGTGGATG ACTCAAAAAT CGTCAGTGAC ATGCTAATGA CCATCTGTGC CAGAATAGGC 
ATCACAAATT ATGATGAGTA CTCACTGGTG CGTGACGTGG GAGAGGAAAA GAAGGAAGAG 
ACCACAGGAA CACTGAAGAG AGACAAGACC CTCCTGAGAG ATGACAAAAA AATGGAGAAA 
CTCAAACAGA AACTTCACAC AGATGACGAG CTGAATTGGT TGGATCATGG GCGGACGCTG 
CGGGAGCAGG GTGTCGAGGA GTCTGAGATG CTGCTTCTTA GGAGAAAGTT CTTCTACTCA 
GACCAGAATG TGGATTCCAG AGACCCTGTA CAGCTCAACC TGCTGTATGT GCAGGCCCGT 
GATGACATTT TGAACGGCTC TCACCCTGTC TCCTTCGATA AGGCCTGTGA GTTTGCTGGA 
TACCAGTGCC AGATTCAGTT TGGAGACCAC AATGAGTCCA AACACAAACC TGGATTCCTA 
GATTTGAAGG AATTCTTGCC CAAAGAGTAC GTCAAGAACA AGGGCGAGAA GAGGATCTTT 
CAGGCACACA AAAATTGTCA AAACATGACC GAGATCGAGG CCAAAGTCAA CTATGTCAAA 
CTAGCCAGAT CTCTGAAAAC ATACGGAGTG TCCTTCTTCT TAGTCAAGGA AAAAATGAAA 
GGAAAGAATA AGCTCGTGCC GCGTTTGTTG GGCATCACTA AAGAGAGTGT GATGAGAGTG 
GATGAGAAAA CCAAAGAGGT GATTCAGGAG TGGAGTCTGA CCAACATCAA ACGCTGGGCA 
GCTTCTCCTA AGAGCTTCAC TCTGGACTTT GGAGACTATC AGGATGGTTA CTACTCAGTT 
CAGACCACTG AAGGAGAACA GATTGCACAA CTCATTGCTG GATACATTGA CATCATTCTC 
AAAAAGAAAA AGAGCAAAGA TCACTTCGGC CTGGAGGGAG ATGAAGAGTC AACCATGTTG 
GAGGACTCCG TCTCTCCGAA AAAGTCAACA GTTCTTCAGC AGCAGTTTAA TAAGATGGGG 
AAGGTGGAGA CGGGTTCTGT CGCTCTACCG GCCATCATCC GATCTGGTGC TGGAGGTCCA 
GAGAGCATGA AGATGGGCTC AATGCCACAG GCCAAACAGC ACATCACCAG CGGTCAGATG 
CACAGAGGAC ATATGCCCCC TCTGACTTCA GCTCAGCAGG CTTTAACTGG CACCATTAAC 
TCCAGCATGC AGGCAGTACA GGCGGTTCAG GCTTCTCTTG ATGAGTTTGA TGCCCTGCCT 
CCACTGGGGA CTGATTCGGC ATCTCAAGCC TGGAGGAAGA ACAAAATGGA TGAGTCCAAA 
CACGAGATTC ACTCTCAGGT GGATGCCATC ACTGCGGGAA CTGCTTCCAT GGTCAATCTG 
ACTGCAGGTG ACCCGGCCGA GACAGATTAC ACTGCTGTGG GCTGTGCCGT CACCACCATC 
TCCTCCAACC TGACGGAGAT GTCCAAGGGT GTCAAACTGC TGGCGGCGCT GATGGAGGAC 
GAGGGCGGAA ATGGCCAGCA GCTTCTGGGT GCTGCAAAGA ACCTGGCGTG TGCCGTGTCT 
GATATGCTCA AGACTGCTCA GCCGTCCAGC ACTGAGCCAC GACAGAACCT CCTGCAAGCA 
GCGGGTAATG TGGGTCAGGC CAGCGGTGAA CTCCTGCAGC AGATCGGGGA GTCTGATGCG 
GATCAGCACT TCCAGGACAT GTTGATGCAG TTGGCGAAGG CTGTGGCGAA TGCAGCAGCG 
GCTCTGGTGT TAAAGGCTAA AAACGTGGCT CAGAAAAGCG AAGATTCAGC TCAACAAAAC 
AAAGTGATCG CTGCTGCCAC CCAGTGCGCC CTCTCCACAT CACAGCTGGT GGCCTGCACA 
CGAGTGGTGG CTCCAACCAT CAGCTCACCC GTGTGTCAAG AGCAGCTCAT CGAGGCTGGA 
AAACTGGTGG CGAAGTCTGT GGAGGGCTGT GTGGAGGCGT CACACGGGGC GACCGGTGAC 
GAGCATCTGC TCAAACAGGT GGGCGCCGCT GCCACAGGAG TCACTCAGGC TCTTAACGAG 
CTGCTGCAGC ATATTAGACA GTACGCCAGC GGAGGACAGC CAATCGGACG GCACGGAGAG 
GCTACCGACC GCATCCTGGA TGTGACTGAT AACATCTTCA GCTCCATGGG AGATGCAGGG 
GAGATGGTGC GTCAGGCGCG GATCCTTGCT CAGGCCACCT CAGACCTGGT CAACGCGATC 
AAGGCGGACG CAGAGGGAGA ATCTGACCTG GAGAACTCTC GCAAACTCCT CTCTGCAGCC 
AAACTCCTGG CTGACGCTAC TGCCCGTATG GTGGAAGCTG CGAAGGGTGC AGCTGCTAAT 
CCAGACAGCG AGGAGCAGCA GCAGAAGTTG CGTGAAGCTG CAGAGGGACT GCGCATGGCC 
ACCAATGCTG CAGCACAGAA CGCCATTAAA AAGAGACTCG TCAACAAACT GGAGAATGCA 
GCGAAACAAG CCGCAGCAGC CGCCACTCAG ACCATAGCAG CCGCTCAACA TGCAGCTTCA 
TCCAACAAGA ACCCTGCGGC CCAGCAACAA CTCGTACAGA GCTGCAAGGT GGTGGCGGAT 
CAGATCCCAC AGCTGGTTCA GGGTCTGAGG AGCAGCCAGG CTCAACCTGA CAGCCCCAGC 
GCTCAACTCG CCCTCATCGG AGCTAGCCAG AACTTCCTGC AGCCCGGAGC CAAAATGGTG 
ACCGCAGCCA AAGCTACAGT TCCAACCATC AGTGACCAGG CGTCTGCCAT GCAGCTCAGT 
CAGTGCGCTA AAAACCTGGC CAGTGCTCTG GCTGAGCTCC GCACGGCCGC CCAGAAGGCT 
CAGGAGGCGT GTGGTCCGTT GGAGATTGAT AACGCTCTGA CTATGGTACG GGGGCTGGAG 
AGGGACATGC AGGAGGCCAA AGCTTCAGCT GCTGAGGGCA AACTCAGACC CCTGCCTGGA 
GAAACGCTGG AGAAGTGCTC TCAGGATCTG GGCTCCAGCA CTAAAGCCGT GAGCTCTGCC 
ATTGCTCAGC TCCTCAGTGA AGCAACTCAA GGCAATGAGA ACTACACAGG TATGGCTGCT 
CGTGATGTGG CTCAGGCTCT GCGCTCGCTG GCTTCAGCCG CTCGTGGAGT GGCAGCCAAC 
ACTGAAGATC CTCTAGCCCG TAACGCAATG CTGGACTGCA CCGGCGATGT CATGGACAAA 
TCCGCCAGCC TGATTGAAGA GACCAAGAGG GCCATAGCCA AACCAGGAGA CCCTGACAGT 
CAGCAAAGAC TGGCCCAGGT AGCCAAGGCT GTGTCTCAGG CACTGAACCG CTCTGTAAAC 
TGTCTGCCGG GGCAGAGGGA TGTGGATAAT GCCATCAGAA CTGTCGGGGA AGCTAGCAAG 
AAACTGCTGT CTGATAAATT TCCAGCGAGT GGGAAGAATT TCCAAGAGGC TCAGGCCAAT 
CTGAACGAGG CTGCAGCTGG ACTAAACCAG TCAGCCAATG AGCTGGTGCA GGCCTCGCGT 
GGCACCACCC AGGACCTGGC CAAAGCCTCC GGGAAGTTTG GACAAGACTT TAACCAATTC 
CTGCAGGCTG GAGTGGACAT GGCTGGACAA TCCCAGTCTA AAGAAGACCA AACTCAAGTG 
GTCACCAACC TGAAGACCAT CTCCATGTCC TCCAGCAAAC TCCTGCTGGC GGCTAAAGCT 
CTGTCCACAG ACCCCAACTC GCCAAATCTG AAGAACCAAC TCGCTGCTGC TGCTCGCGCG 
GTGACAGACA GCATTAATCA GCTGATCAGC ATGTGTACCC AGCAGGCTCC GGGACAGAAG 
GAGTGTGACA ACGCTCTCCG TGAGCTTGAG ACGGTTGGAG GAATGCTGGA GAACCCAACA 
GAAGCAGTCA ATGATTTGGG CTACTTTGAC TGCATTGACA GTGTGATGGA GAACTCAAAG 
GTTTTGGGTG AGGCCATGGC TGGCATTTCC CATAATGCCA AAAACAGTAA CTTGCCAGAG 
TTTGGAGATT CGGTCAGTTC AGCGTCTAAA GCGCTGTGTG GTCTCACTGA AGCAGCTGCT 
CAGGGGGCGT ATCTGGTTGG TGTGTCAGAC CCGAACAGCC ATGCTGGCCA GAAGGGTCTT 
GTGGACCCCT CACAATTTGC CAGAGCCAAA CAGTCCATCC AGATGGCCTG TCAGAACCTG 
GTGGATCCAG CCTGCACCCA GTCCCAGGTT CTCTCTGCTG CCACCATCGT TGCCAAACAC 
ACTTCGGCCT TGTGCAATGC CTGCCGGCTC GCCTCCTCCA AGACATCCAA CCCAGTTGCT 
AAGAGACAGT TCGTCCAGAG TGCCAAAGAG GTGGCCAACA GCACAGCCAA CCTCGTCAAG 
TCCATCAAGG CTCTCGACGG GGCCTTTAAC CAGGAAAACA GACAGAAGTG TAAGGAAGCC 
ACTGGGCCAC TCATTGAGGC TGTGGATAAC CTGACTGCCT TCGCTTCAAA CCCAGAGTTC 
GCAAGTGTAC CCGCTCAGAT CAGCCCAGAG GGTCTGGCTG CGATGGAGCC GATCACAGCA 
GCCGCTAAGG CAATGTTGGA AAGCTCGACA GGACTGATCC AGACGGCTCG CTCGCTCGCA 
GTCAACCCGA AAGATCCACC CAAGTGGTCT GTGCTTGCTG GACACTCCAG AACAGTGTCC 
GACTCCATCA AGAAACTCAT CACTAACATG AGGGAAAAGG CTCCGGGTCA GAGGGAGTGT 
GATGATGCCA TTGAGGTTCT TAGTAACTGC ATTCGTGAAG TGGACAAGGC CTCGCTGGCA 
GCCATCAGTC AGCAGCTCAC GCCTAGAGAC GACATTTCCC ATGAGGCTCT GCACGAGCAG 
ATGGCGGCGT CTGTGCATGA GATCAGTAAC CTGATTGAAC CAGTGGCCAT CGCAGCCCGT 
GCTGATGCCT CTCAGCTGGG ACACAAGGTG TCTCAGATGG TCAGTTACTT TGAACCGCTC 
ATCATGGCTG CCATCGGCAC AGCGTCTAAG ATCCTGAACA GCCAGCAGCA GATGAACGTG 
CTGGACCAAA CCAAGACTCT GGCTGAGTCT GCGCTACAGA TGCTCTATAC GGCCAAAGAG 
ACTGGAGGAA ACCACAAGGC GGCGCACACA CAAGAGGCTC TGGAGGAGTC TGTTCAGATG 
ATGAAGGAGG CGGTGGACGA TCTGGGTTCG ACTCTGGCAG AAGTGGCCAG CGCTGCAGGA 
GCAGTGGGAG GAATGGTCAA CTCCATCACT CAGTCCATCA ACAAGATGGA GGACGGACCT 
GCCTTCGAGC CTGAAGGAAC GTTTGTGGAT TATCAGACCA CCATGGTGAA GACAGCCAAA 
GCTATCGCTG TCACCGTTCA GGAGATGGTT ACCAAATCCA ACACCAACCC TGATGAGCTG 
GGCGGTTTGG CCAATCAGCT GACCACAGAG TTCGGTGACC TCGCATCAGA GGCCAAATGT 
GCAGCCATGA CTGCTGAAAA CGATGAGATC GGTTCACATA TCAAGAAGCA GGTGACTGAG 
TTGGGCTACA CCTGCACTGG TCTGGTCAGT AAAGCAGGAG CGCTACAGTG CAGTCCAAAC 
GACTCCTACA CTAAGAAGGA GCTCATTGAC AGTGCCAGGA AAGTCTCTGA GAAGGTCTCT 
CATGTGCTGG CAGCTCTTCA GGCAGGCAAT CGTGGCACTC AGGCCTGCAT TACTGCCGCG 
AGCGCTGTCT CTGGCATCAT TGCTGATTTA GACACCACCA TCATGTTTGC CACCGCGGGC 
ACTCTCAACC GAGAGAACGC AGAGACATTT GCAGACCACA GAGAGCACAT CTTAAAGACG 
GCTAAAGTTC TGGTGGAGGA CACGAAGCTG CTGGTTTCTG GAGCCGGAGC AAGTCAGGAG 
AAACTGGCTC AGGCTGCACA GTCCTCCGTC AACACCATCA CCAAACTGGC CGATGTGGTT 
AAATTGGGGG CAGCGAGTCT GGGCTCTGAA GACCCAGAGA CACAGGTGGT TCTGATCAAC 
GCTGTGAAGG ACGTGGCTAA AGCTCTGGGT GACCTGATCA GCGCCACCAA AGCAGCGGCC 
GGCAAACCTC ACGATGACCC CGCCATGCTG CAGCTCAAAA ACTCTGCTAA GGTGATGGTG 
ACCAATGTGA CGTCTCTACT TAAGACGGTG AAGGCGGTGG AGGATGAAGC CACTAAAGGC 
ACACGAGCAC TAGAGGCCAC CATAGAGCAC ATCAAACAAG AGCTGGCTGT TTTCAGCAGT 
GCAGATCCTC CTCCAAAGAC GGCCACTCCA GAGGAGTTCA TCCGCATGAC CAAAGGCATC 
ACATTGGCCA CAGCTAAAGC AGTGGCTGCT GGGAACTCCT GCCGTCAGGA GGACGTGATC 
GCCACTGCTA ACCTAAGCAG ACGAGCTGTC GCAGACATGC TGCGCTCCTG CAAGCAAGCC 
GCATACCACC CGGAGGTGAA GCGGGAGGTC CAGACTCGAG CGCTCCGCTT TGGCTCTGAG 
TGCTCCACAA GTTACCTGGG CCTGCTGGAG CACGTGTTAG TGATCATTCA GAAACCCTCG 
CATGACCTTA AACAGCAGCT GGCAAACTAC TCCAAACGTG TGGCCAGTTC AGTGACCGAG 
CTCATCCAGG CTGCGGAGGC CATGAAAGGT ACGGAGTGGG TGGATCCCGA AGACCCCACC 
GTCATTGCAG AAAACGAGCT GCTGGGAGCC GCAGCTGCCA TTGAAGCTGC CGCGAAGAAA 
CTAGAGCAGC TCAAACCCCG AGCCAAACCC AAGGAAGCTG ATGAGAGTCT GAACTTTGAG 
GAACAGATTC TAGAAGCGGC CAAATCCATC GCTGCAGCCA CCAGTGCTCT GGTCAAAGCT 
GCGTCTGCCG CTCAGAGAGA GCTGGTGGCT CAAGGGAAGG TTGGGGCAAT TCCAGCAAAC 
GCGGTGGATG ATGGACAGTG GTCTCAGGGT CTCATTTCAG CTGCTCGTAT GGTAGCCGCA 
GCTACTAACA ACCTGTGCGA AGCAGCTAAC TCTGCGGTTC AGGGTCACGC CAGCGAGGAG 
AAACTCATTT CTTCAGCCAA ACAGGTGGCA GCATCCACCG CACAGCTGCT GGTGGCCTGT 
AAGGTCAAGG CTGACCAGGA CTCCCAGACC ATGAAGAGAC TGCAGGCTGC TGGTAATGCT 
GTCAAGAGGG CGTCTGATAA CCTGGTGAAG GCGGCACAGA AGGCTGCGTT TGAGGCTCAA 
GATGATCAGG CTGTGATGGT GAAGAGCAAG ATGGTGGGCG GTATTGCTCA GATCATCGCT 
GCTCAGGAGG AGATGCTCAG GAAGGAGAAA GAGCTGGAAG AAGCCAGAAA GAAACTGGCA 
ATGATCCGAC AGCAGCAGTA CAAGTTCCTG CCCTCAGAGC TGCGAGAGGA CGGACAGGAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

The hyaloid vasculature facilitates basement membrane breakdown during choroid fissure closure in the zebrafish eye.
Developmental biology419(2)262-272(2016 11)
James A,Lee C,Williams AM,Angileri K,Lathrop KL,Gross JM

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tln1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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